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... Although the quantification of key microorganisms in fermentation microbiomes is important for monitoring and regulating fermented food production, conventionally used methods are generally time-consuming, involve complicated operations, and have a high level of equipment dependence. We developed a CRISPR-Cas12a-based quantitative Chinese Baijiu brewing microorganism assay (CQAOB) for Lactobacillu ...
... We explored a low-cost wireless smart portable sensor based on a disposable laser-induced porous graphene (LIPG) flexible substrate electrode decorated by gold nanoshells (AuNSs) for a simple and rapid electrochemical detection of sulfonamides (SAs). The LIPG was prepared by the one-step computer-controlled laser direct-writing patterning techniques on polyimide (PI) substrate, and the AuNSs were ...
... A selective and sensitive novel reverse-phase method was developed and validated successfully using waters acquity ultra-performance liquid chromatography H-class system coupled with waters Micromass Quattro Premier XE (UHPLC–MS/MS) for the identification and quantification of bis (2-chloroethyl) amine genotoxic impurity in aripiprazole drug substance. The developed method employs a positive-ion e ...
absorption; agrochemicals; aqueous solutions; density functional theory; detection limit; electron transfer; energy; food contamination; green chemistry; human health; irradiation; ligands; luminescence; paraquat; rapid methods; toxicity
Abstract:
... The toxic agrochemical contamination of food and the environment is one of the most pressing concerns for human health and environmental sustainability. In this work, a water-stable 3D lanthanide-based MOF, [Tb₂(H₂btec)(btec)(H₂O)]·4H₂O (Tb-MOF) has been demonstrated as a turn-off luminescent sensor for the detection of poisonous paraquat. The ligand 1,2,4,5-benzenetetracarboxylic acid (H₄btec) wa ...
... A dual-response ratiometric fluorescence imprinted sensor based on visible/near-infrared emission was established for ultrasensitive, selective and visual detection 4-nitrophenol (4-NP). The molecularly imprinted polymer was incorporated in the ratiometric sensing system consisting of visible emission carbon dots@zeolitic imidazolate framework-8 (CDs@ZIF-8) and near-infrared emission cadmium tellu ...
... A microfluidic biosensor was developed for rapid detection of Salmonella using a finger-actuated micropump, a finger-actuated micromixer, gold@platinum nanocatalysts (Au@PtNCs) and a smartphone App. First, immune magnetic nanobeads (MNBs), bacterial sample and immune Au@PtNCs were successively finger-actuated pumped into microfluidic chip. Then, they were fully mixed using finger-actuated micromix ...
... A fluorescence and surface-enhanced Raman scattering (FL-SERS) dual-mode aptasensor was established for sensitive and rapid detection of Ochratoxin A (OTA). This aptasensor was assembled by the hybridization of OTA aptamer (OTA Apt) modified gold nanostars (Apt-AuNSs) and Cy3 modified complementary DNA functionalized gold nanospheres (cDNA-AuNPs). The aptasensor displays a low FL signal due to the ...
... Salmonella, as a common foodborne pathogen in dairy products, poses a great threat to human health. We studied a new detection method based on quantum dots (QD). A fluorescent biosensor with multiple fluorescent signal amplification based on a streptavidin (SA) biotin system and the polyamino linear polymer poly-l-lysine (PLL) were established to detect Salmonella in milk. First, Salmonella was ca ...
... There is a critical need for the rapid detection of p-chlorophenol produced by pesticide abuse and industrial wastewater discharge, which has been an urgent problem in the realm of environmental protection. Here, a green and environmentally friendly method was developed to prepare stable and low toxicity quantum dots. First, blue-green fluorescent FeS₂ quantum dots (B-FeS₂ QDs) were prepared with ...
... Foodborne diseases caused by Yersinia enterocolitica serotype O:8 represent global public health problems. We need rapid Y. enterocolitica O:8 detection methods to ensure food safety. In this study, we developed a colloidal gold-based immunochromatographic strip test (ICST) for the rapid detection of Y. enterocolitica O:8. A sandwich detection format was applied to Y. enterocolitica O:8 detection, ...
acetamiprid; aptasensors; cabbage; color; colorimetry; cucumbers; detection limit; gold; mobile telephones; nanogold; oligonucleotides; quantitative analysis; rapid methods; river water
Abstract:
... Based on the colorimetric analysis of gold nanoparticles and a smartphone readable strategy, a stable, sensitive, and visual method was established for rapid detection of acetamiprid residues in agricultural products. By optimizing the key parameters, the detection process only took 40 minutes with good specificity. The acetamiprid aptamer can help AuNPs to resist salt-induced aggregation. Convers ...
... Cell-free nucleic acids (cfNAs) such as short non-coding microRNA (miRNA) and circulating tumor DNA (ctDNA) that reside in bodily fluids have emerged as potential cancer biomarkers. Methods for the rapid, highly specific, and sensitive monitoring of cfNAs in biofluids have, therefore, become increasingly attractive as clinical diagnosis tools. As a next generation technology, we provide a practica ...
... A novel closed-tube loop-mediated isothermal amplification (LAMP) was developed to detect goat milk adulterated with cow milk. The reaction results of the LAMP assay developed, identified using both visual observation and agarose gel electrophoresis, were completely consistent, indicating that visual observation was suitable and efficient to judge the final LAMP results. The specificity of LAMP as ...
aptasensors; chitosan; colorimetry; detection limit; kanamycin; milk; nanocomposites; oligonucleotides; oligosaccharides; rapid methods; tap water
Abstract:
... Herein, the split aptamers, chitosan oligosaccharide, and AuNPs were combined as nanocomposites that present different formations to develop a label-free colorimetric aptasensor for rapid detection of small molecules. Kanamycin was chosen as a model target. Computational studies were performed to assist in the design of orientated immobilization of the split aptamers onto the AuNPs surface. Chitos ...
... The control of pesticide residues in food is of increasing importance nowadays due to the over-use and misapplication of herbicides in agricultural production. However, the current colorimetric method for rapid detection of glyphosate still faces many challenges like the low sensitivity and stability. Herein, a simple and ultrasensitive liquid colorimetric chemosensor for glyphosate detection was ...
... The detection of cancer markers still has shortages of low sensitivity, time-consuming operation, the use of unstable and expensive antibodies. In this work, a novel Love-mode surface acoustic wave (LSAW) aptasensor with dummy fingers based on the monolayer molybdenum disulfide/gold nanoparticles (monolayer MoS₂/Au NPs) was developed for the highly sensitive and rapid determination of alpha-fetopr ...
antioxidants; black tea; detection limit; electric current; food chemistry; gallic acid; humans; rapid methods; xenon
Abstract:
... Gallic acid (GA) is found in a wide range of natural plants and is relevant to the health of human beings. Here, a photoelectrochemical sensing platform based on g-C₃N₄@CNT heterojunction has been prepared for the highly sensitive and selective detection of GA. Under the light of xenon lamp, the photocurrent of g-C₃N₄@CNT is 7 times higher than that of g-C₃N₄. And the sensor generates 4 times more ...
... Rapid screening and early warning of pathogenic bacteria in food is the key to prevent and control the spread of foodborne diseases. Biotin–streptavidin system and membrane filtration technology were combined to develop a novel nuclear magnetic resonance (NMR) immunosensor based on biomacromolecule O-carboxymethyl chitosan (O-CMCS) target gadolinium (Gd) probe for rapid detection of Salmonella ana ...
... The overuse of antibiotics has aroused widespread concern in recent decades. Their residues in food and environment may pose potential risks to human health. Therefore, highly sensitive and rapid detection methods of antibiotics are urgently needed. Inspired by allosteric transcription factors (aTFs), we proposed a novel strategy for small molecules detection based on antibody controlled isotherma ...
... Aflatoxin M₁ (AFM₁) is a common toxin in dairy products that causes acute and chronic human health disorders. Thus, the development of a rapid and accurate AFM₁ detection method is of vital importance for food safety monitoring. This work was to develop a novel electrochemical aptasensor for sensitive and specific determination of AFM₁. The dendritic-like nanostructure was formed on the gold elect ...
... Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe₃O₄@graphene modified electrode. Because of the excellent electrical conductivity and mechanical stability of graphene and the large specific surface area of Fe₃O₄, the Fe₃O₄@graphene nanocomposite exhibits an excellen ...
... Listeria monocytogenes is an important foodborne pathogen worldwide, with 20–30% fatality rate in vulnerable persons. The hypervirulent L. monocytogenes clonal complex (CC) 87 strains have emerging both in food production environments and clinic cases. The objective of this study was to develop a multiplex PCR to simultaneously detect L. monocytogenes CC87 and CC88 strains based on pan-genome anal ...
... The detection of nitrite is of great significance because it is closely related to food safety. In this work, a rapid colorimetric method was developed for nitrite detection based on the reaction of propylene glycol alginate (PGA) gel interface. In the reaction of nitrite and 4-Aminoacetophenone, diazo compound formed, which could be further transformed to purplish red compound by reacting with N- ...
... (SVA), an emerging picornavirus, has been associated with vesicular disease and neonatal mortality in swine, posing a great threat to the global swine industry. Accurate diagnosis of SVA is crucial for the effective prevention and control disease. In the present study, a simple, rapid and accurate diagnostic assay was developed combining recombinase polymerase amplification and a lateral flow dips ...
Salmonella; aerosols; biosensors; cross reaction; detection limit; dried skim milk; drug resistance; drug therapy; liquids; milk; rapid methods; salmonellosis; virulence
Abstract:
... Accurate, sensitive, and rapid detection of Salmonella and determination of whether it carries drug resistance genes plays an important role in guiding the clinical medication of salmonellosis and laying a foundation for studying the mechanism of drug resistance transmission of Salmonella. Here, a novel nontransferable, ultrasensitive dual detection platform (Cas12a-Ddp) was developed. The round c ...
... Occupational health problems, such as asthma, in specific work environments arise from the presence of airborne fungi. Rapid detection of pathogenic airborne fungi is therefore important to reduce or avoid any adverse effects on staff health. Herein, we established a new integrated rapid Lyticase-Motor-Chemical reagent nucleic acid releasing (LMC) method for the release of fungal DNA. Aspergillus ...
... A label-free electrochemical immunosensor has advantages of real-time and rapid detection, but it is weak in detection of small molecular toxins such as aflatoxin B₁ (AFB₁). The greatest obstacle to achieving this is that small molecules bound to a common immunosensing interface cannot interfere with electron transfer effectively and the detection signal is so weak. Therefore, a sensitive electroc ...
... A fluorescent aptasensor based on sensitized terbium(III) luminescence was constructed to detect melamine in milk. Tb³⁺ as the fluorescence probe can be sensitized by a guanine-rich single-stranded DNA sequence, so the complementary sequence of the polythymidine aptamer (cDNA) was modified with six consecutive guanine bases (G6). In the absence of melamine, melamine aptamer combined with cDNA to f ...
carbon nanotubes; carboxymethylcellulose; chemical bonding; computers; dendrimers; detection limit; electrochemistry; feeds; food composition; glassy carbon electrode; immunoassays; immunosensors; mobile telephones; monoclonal antibodies; nanocomposites; pathogenicity; public health; rapid methods; zearalenone
Abstract:
... Zearalenone (ZEN) has emerged as a global public health concern due to its significant pathogenicity and world-wide distribution in animal feed ingredients and agricultural food. In the study, we report on the design and development of a simple portable immunosensor based on polyamindoamine dendrimer (PD)-multi-walled carbon nanotube (MWCNT) nanocomposite containing carboxymethyl cellulose (CMC) f ...
... The “Horsemeat Scandal” makes people pay more attention to the meat authenticity. However, expensive equipment, complicated operations, and professional personnel of current methods limit their field testing. In this study, CRISPR/Cas12a combined with recombinase polymerase amplification was used to establish a sensitive and rapid detection method for pig-derived component. The detection limit can ...
aptasensors; biomarkers; blood serum; chemical species; detection limit; electric field; electrochemistry; electroosmosis; hybridization; immunosensors; oligonucleotides; rapid methods
Abstract:
... Traditional immunosensors are often limited by low sensitivity and long detection times, for they usually depend on passive diffusion-dominated transport of target analytes for the binding reaction with a bio-recognition element such as enzymes, antibodies, and aptamers. Numerous studies rely on electric field manipulation by using alternating current (AC) electrokinetics to enhance the hybridizat ...
... As common toxigenic fungi genera, Aspergillus, Penicillium, and Fusarium, have attracted the attention of governments and people all over the world due to their human carcinogenicity, teratogenicity, and hepatotoxicity. Accordingly, effective and quantitative detection method for toxigenic fungi before mycotoxins produced should be established to ensure food safety. In this paper, two duplex-dropl ...
... Listeria monocytogenes is an infectious foodborne pathogen that greatly threatens the public health worldwide. A simple and sensitive detection of L. monocytogenes is extremely important in food safety industry. In this study, we developed an all-in-one platform, which consists of nucleic acid extraction, amplification and visual detection in a single tube. Following a simple bacterial lysis, the ...
... Oxadixyl is a systemic phenylamide fungicide, used to prevent and control disease caused by phytophthora and downy mildew in a variety of cash crops. In this study, a hapten of oxadixyl was designed and used to prepare a highly specific and sensitive anti-oxadixyl monoclonal antibody (mAb) with a 50% inhibition concentration of 3.68 ng/mL. An immunochromatographic assay (ICA) was developed for scr ...
... A turn-on fluorescent sensing platform based on an ultralow concentration of Al-metal organic frameworks for the detection of aflatoxin B₁ has been developed for the first time. This fluorescence turn-on sensor exhibits the largest fluorescence enhancement (or quenching) constant value of 179404 M⁻¹ among all luminescence-based chemical sensors reported till date. Moreover, the sensor afforded a r ...
... In this study, a novel hapten was designed and successfully synthesized to prepared the artificial immunogen against xylazine (XYL), then the monoclonal antibody 1H2-2C6 was obtained and applied to a lateral flow immunoassay (LFI) strip for rapid detection of XYL in milk. The visual detection limit (cutoff value) of the LFI strip was 5 ng/mL, the linear regression formula was Y = −0.521lg(X) + 0.3 ...
... In this study, a special poly solid-phase extraction (in-tube SPE) column consisting of poly (POSS-octavinyl-co-N-methylacetamide-co-divinylbenzene) [poly (POSS-OS-co-DVB-co-NMA)] was prepared based on the chemical structure of the preservatives, and was used as medium for extraction analysis in combination with UPLC. The composition of polymer SPE was optimized and characterized; good scanning el ...
... Rapid, mass diagnosis of the coronavirus disease 2019 (COVID-19) is critical to stop the ongoing infection spread. The two standard screening methods to confirm the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are polymerase chain reaction (PCR), through the RNA of the virus, and serology by detecting antibodies produced as a response to the viral infection. However, given the dete ...
... Cholera is a severe diarrheal disease caused by Vibrio cholerae, a natural inhabitant of brackish water. Effective control of cholera outbreaks depends on prompt detection of the pathogen from clinical specimens and tracking its source in the environment. Although the epidemiology of cholera is well studied, rapid detection of V. cholerae remains a challenge, and data on its abundance in environme ...
... Increased use of pesticides in agriculture requires new advanced techniques to monitor both environmental levels and human exposure of pesticides to avoid potential adverse health outcomes in sensitive populations. Atrazine is widely used to control broadleaf weeds, and here we developed a new sensor capable of detecting diaminochlorotriazine (DACT), the major metabolite and biomarker of atrazine ...
... Multiplex electrochemical biosensors have been used for eliminating the matrix effect in complex bodily fluids or enabling the detection of two or more bioanalytes, overall resulting in more sensitive assays and accurate diagnostics. Many electrochemical biosensors lack reliable and low-cost multiplexing to meet the requirements of point-of-care detection due to either limited functional biosensor ...
... Guanine is the most susceptible to oxidation among all the DNA bases, and 8-oxo-7,8-dihydroguanine (OG) is one of main oxidation products that can occur in any part of chromosomal DNA. OG in the telomere sequence is associated with telomere shortening, cell aging, and dysfunction, and it may induce cancers. The accurate detection of OG in telomeres is important to early clinical diagnosis and mole ...
... Vibrio fluvialis is an emerging foodborne pathogen that causes severe infections. Serotyping based on surface polysaccharide antigens is important for the clinical detection and epidemiological surveillance of pathogens such as V. fluvialis. For example, variation of the O-antigen, which is highly polymorphic and is responsible for the majority of antigenic variability on the bacterial cell surfac ...
... A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. An anti-profenofos polyclonal antibody (pAb) was success ...
... In this paper, a simple and effective strategy was designed for colorimetric sensing of benzoyl peroxide (BPO) based on the morphological transition of silver nanoprisms (AgNPRs). The decomposition of O–O bond of BPO generated strong oxidant benzoyl oxide free radical, which could be first oxidized the silver atoms at the corner areas and the {110} facet of AgNPRs. With the increase of the BPO con ...
... Foodborne diseases caused by Listeria monocytogenes (L. monocytogenes) occur frequently. The rapid, ultrasensitive, and visual methods are urgently needed for the detection of viable L. monocytogenes. We developed a novel lateral flow strip for visual and quantitative detection of viable L. monocytogenes cells and biofilms based on propidium monoazide-loop-mediated isothermal amplification (PMA-LA ...
... The development of a sensitive and rapid screening method for Ralstonia solanacearum is critical for the control of tobacco wilt. In the present study, tissue homogenates of three tobacco varieties (Honda, Yunnan 87 and K326) with different resistance to R. solanacearum, were individually used as additives to the bacteria culture medium. The changes in R. solanacearum secretome were investigated a ...
... We present the first combination of a microfluidic polymerase chain reaction (PCR) with a gold nanoslit-based surface plasmon resonance (SPR) sensor for detecting the DNA sequence of latent membrane protein 1 (LMP1). The PCR microchannel was produced through a laser scribing technique, and the SPR nanoslit chip was manufactured via hot-embossing nanoimprinting lithography. Afterward, the LMP1 DNA ...
... Micro-nano heterogeneous oxides have received extensive attention due to their distinctive physicochemical properties. However, it is a challenge to prepare the hierarchical multicomponent metal oxide nanomaterials with abundant heterogeneous interfaces in a controllable way. In this work, the effective construction of the heterogeneous structure of the material is achieved by regulating the ratio ...
... Colorimetric and chemoresistive gas sensing methods have aroused great interest in H₂S monitoring due to their unique merits of naked-eye readout, and highly sensitive and rapid detection. However, combining these two methods for gas detection, especially utilizing one material as their common sensing material is a grand challenge because they are inconsistent in sensing mechanism. Taking advantag ...