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1. Quantitative detection of viable Escherichia coli O157:H7 using a photoreactive DNA-binding dye propidium monoazide in irrigation water

Author:
Huang, Min,   et al.   ; Xie, Guoyang; Hu, Liehai; Liu, Rui; Liang, Taobo; Aguilar, Zoraida P.; Xu, Hengyi;     Show all 7 Authors
Source:
Biochemical engineering journal 2019
ISSN:
1369-703X
Subject:
DNA; Escherichia coli O157; bacteria; cell membranes; cell viability; confocal laser scanning microscopy; detection limit; dyes; genes; irrigation water; pathogens; permeability; propidium; quantitative polymerase chain reaction
Abstract:
... Escherichia coli O157:H7 is a kind of waterborne pathogen and recognized as a common pathogenic microorganism in irrigation water. In this study, a quantitative PCR (qPCR) assay with propidium monoazide (PMA) treatment called PMA-qPCR assay was developed to detect the presence of E. coli O157:H7 in natural irrigation water that was more specific, more sensitive, and was unaffected by dead cells co ...
DOI:
10.1016/j.bej.2019.107354

2. A liquid chromatography with tandem mass spectrometry method to simultaneously determinate chlorpyrifos, imidacloprid and imidacloprid metabolites in wheat

Author:
Huang, Min,   et al.   ; Qin, Xinxian; Luo, Xiaoshuang; Yu, Weiwei; Yang, Guoqiang; Zhang, Kankan; Hu, Deyu;     Show all 7 Authors
Source:
Journal of separation science 2019 v.42 no.6 pp. 1210-1221
ISSN:
1615-9306
Subject:
chlorpyrifos; crops; detection limit; imidacloprid; liquid chromatography; metabolites; olefin; soil; standard deviation; tandem mass spectrometry; urea; wheat; wheat straw
Abstract:
... A liquid chromatography with tandem mass spectrometry method was developed and validated to simultaneously determine chlorpyrifos, imidacloprid, and imidacloprid metabolites in soil, wheat grain, and wheat straw matrices. Satisfactory linearity (R² ≥ 0.9965) of the method was obtained for all analytes. The ranges of limits of detection and limits of quantification for seven analytes in three matri ...
DOI:
10.1002/jssc.201801163

3. The Development and Validation of a Liquid Chromatography–Tandem Mass Spectrometry Procedure for the Determination of Dioctyldiethylenetriamine Acetate Residues in Soil, Green and Cured Tobacco Leaves Using a Modified QuEChERS Approach

Author:
Huang, Min,   et al.   ; Gong, Jin; Yang, Guoqiang; Yu, Weiwei; Li, Fuqin; Jin, Mingjiao; Hu, Deyu; Zhang, Kankan;     Show all 8 Authors
Source:
Chromatographia 2018 v.81 no.7 pp. 1035-1041
ISSN:
0009-5893
Subject:
acetates; detection limit; electrospray ionization mass spectrometry; leaves; liquid chromatography; methanol; monitoring; particle size; soil; statistical analysis; tandem mass spectrometry; tobacco
Abstract:
... A liquid chromatography with electrospray ionization tandem mass spectrometry method was developed and validated for the determination of dioctyldiethylenetriamine acetate in soil and tobacco. The separation was performed on a Restek Ultra AQ C₁₈ column (2.1 × 100 mm i.d., particle size 3 µm) at 40 °C with a gradient elution. Methanol and trifluoroacetic acid (0.1%) were used as mobile phase, and ...
DOI:
10.1007/s10337-018-3535-z

4. Simultaneous determination and risk assessment of metalaxyl and azoxystrobin in potato by liquid chromatography with tandem mass spectrometry

Author:
Huang, Min,   et al.   ; Yu, Weiwei; Luo, Xiaoshuang; Qin, Xinxian; Li, Jian; Zeng, Song; Zhang, Kankan; Hu, Deyu;     Show all 8 Authors
Source:
Environmental monitoring and assessment 2018 v.190 no.6 pp. 335
ISSN:
0167-6369
Subject:
acetonitrile; azoxystrobin; detection limit; field experimentation; leaves; liquid chromatography; maximum residue limits; metalaxyl; potatoes; risk; silane; soil; solid phase extraction; sorbents; statistical analysis; tandem mass spectrometry; China
Abstract:
... A liquid chromatography with tandem mass spectrometry method was developed and validated to simultaneously determine metalaxyl and azoxystrobin in soil, potato, and potato foliage samples. The samples were extracted by 20 mL of acetonitrile and purified with dispersive solid-phase extraction using octadecyl silane as sorbent. The method showed good linearity (determination coefficients ≥ 0.9926) f ...
DOI:
10.1007/s10661-018-6717-0

5. Nanoliter-Scale Oil-Air-Droplet Chip-Based Single Cell Proteomic Analysis

Author:
Huang, Min,   et al.   ; Li, Zi-Yi; Wang, Xiu-Kun; Zhu, Ying; Li, Jin-Song; Wong, Catherine C. L.; Fang, Qun;     Show all 7 Authors
Source:
Analytical chemistry 2018 v.90 no.8 pp. 5430-5438
ISSN:
1520-6882
Subject:
detection limit; droplets; human cell lines; hydrophobicity; mice; oocytes; proteins; proteomics
Abstract:
... Single cell proteomic analysis provides crucial information on cellular heterogeneity in biological systems. Herein, we describe a nanoliter-scale oil-air-droplet (OAD) chip for achieving multistep complex sample pretreatment and injection for single cell proteomic analysis in the shotgun mode. By using miniaturized stationary droplet microreaction and manipulation techniques, our system allows al ...
DOI:
10.1021/acs.analchem.8b00661

6. Risk assessment and monitoring of dinotefuran and its metabolites for Chinese consumption of apples

Author:
Huang, Min,   et al.   ; Yu, Weiwei; Chen, Jiaojiao; Wu, Sizhuo; Zheng, Kunming; Zeng, Song; Zhang, Kankan; Hu, Deyu;     Show all 8 Authors
Source:
Environmental monitoring and assessment 2017 v.189 no.10 pp. 521
ISSN:
0167-6369
Subject:
Malus domestica; apples; detection limit; dinotefuran; granules; guanidines; guidelines; liquid chromatography; maximum residue limits; metabolites; monitoring; risk; risk assessment; statistical analysis; tandem mass spectrometry; urea; China; Japan
Abstract:
... Residues of dinotefuran and its metabolites, 1-methyl-3-(tetrahydro-3-furylmethyl)urea (UF) and 1-methyl-3-(tetrahydro-3-furylmethyl)guanidine (DN), in apple were investigated using a “QuEChERS” (quick, easy, cheap, effective, rugged, safe) pretreatment and liquid chromatography–tandem mass spectrometry. Limits of detection (LODs) and quantification (LOQs) of dinotefuran, UF, and DN in apples were ...
DOI:
10.1007/s10661-017-6239-1

7. Rapid quantification of ionophores in feeds by liquid chromatography– tandem mass spectrometry

Author:
Huang, Min,   et al.   ; Rumbeiha, Wilson K.; Braselton, W. Emmett; Johnson, Margaret;     Show all 4 Authors
ISSN:
1943-4936
Subject:
detection limit; horses; lasalocid; liquid chromatography; livestock production; mass spectrometry; medicated feeds; monensin; narasin; nigericin; particle size; salinomycin; veterinary medicine
Abstract:
... Ionophores are widely used in veterinary medicine as coccidiostats and for improving nutrient utilization in livestock production. Because of widespread use, ionophores sometimes cause poisoning in livestock. Quantifying concentration of these compounds in feeds for diagnostic purposes is needed. A method with a single step of solvent extraction was developed for rapid simultaneous quantification ...
DOI:
10.1177/1040638711416965

8. Determination of erythrocyte methotrexate polyglutamates by liquid chromatography/tandem mass spectrometry after low-dose methotrexate therapy in Chinese patients with rheumatoid arthritis

Author:
Huang, Min,   et al.   ; Mo, Xiaolan; Wen, Yuguan; Ren, Bin; Chen, Jie; Xu, Chenshu; Wang, Xueding; Chen, Xiao;     Show all 8 Authors
Source:
Journal of chromatography 2012 v.907 pp. 41-48
ISSN:
1570-0232
Subject:
ascorbic acid; detection limit; erythrocytes; ionization; liquid chromatography; methotrexate; patients; rheumatoid arthritis; tandem mass spectrometry; therapeutics
Abstract:
... Methotrexate (MTX) is currently one of the most widely used drugs for treatment of rheumatoid arthritis (RA) through polyglutamation of methotrexate polyglutamates (MTXPGs), a process attaching sequential γ-linked glutamic residues to MTX. A new and sensitive LC/MS/MS method was developed and validated for determination of whole-blood MTX and total MTX (MTX+MTXPGs), and then concentration of MTXPG ...
DOI:
10.1016/j.jchromb.2012.08.032

9. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood

Author:
Huang, Min Joon,   et al.   ; Zhang, Guo-Jun; Luo, Zhan Hong Henry; Ang, Jun’An Jason; Kang, Tae Goo; Ji, Hongmiao;     Show all 6 Authors
Source:
Biosensors & bioelectronics 2011 v.28 no.1 pp. 459-463
ISSN:
0956-5663
Subject:
adults; antibodies; biomarkers; biosensors; blood; chest; creatine kinase; death; detection limit; electrocardiography; enzyme-linked immunosorbent assay; filtration; hospitals; myocardial infarction; nanowires; pain; patients; silicon; troponin T
Abstract:
... Cardiovascular diseases are the major cause of death among adults worldwide. Electrocardiogram (ECG) is a first test when a patient suffering from chest pain sees a doctor, however, it is lack of the required sensitivity. Standard assays to detect cardiac biomarkers, like enzyme-linked immunosorbent assay (ELISA) are sensitive, but suffer from important sample and reagent consumption in large-scal ...
DOI:
10.1016/j.bios.2011.07.007

10. Seeing protein monolayers with naked eye through plasmonic Fano resonances

Author:
Huang, Min,   et al.   ; Yanik, Ahmet A.; Cetin, Arif E.; Artar, Alp; Mousavi, S. Hossein; Khanikaev, Alexander; Connor, John H.; Shvets, Gennady; Altug, Hatice;     Show all 9 Authors
Source:
Proceedings of the National Academy of Sciences of the United States of America 2011 v.108 no.29 pp. 11784-11789
ISSN:
0027-8424
Subject:
biochemical compounds; biosensors; cameras; detection limit; diagnostic techniques; evaporation; fluorescent labeling; instrumentation; light intensity; spectrometers
Abstract:
... We introduce an ultrasensitive label-free detection technique based on asymmetric Fano resonances in plasmonic nanoholes with far reaching implications for point-of-care diagnostics. By exploiting extraordinary light transmission phenomena through high-quality factor (Qsolution ~ 200) subradiant dark modes, we experimentally demonstrate record high figures of merits (FOMs as high as 162) for intri ...
DOI:
10.1073/pnas.1101910108
PubMed:
21715661
PubMed Central:
PMC3141965