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- Lequarre, A.S.; Grisart, B.; Moreau, B.; Schuurbiers, N.; Massip, A.; Dessy, F.
- Gamete research 1997 v.48 no.2 pp. 216-226
- messenger RNA; metabolism; NADP-glucose-6-phosphate dehydrogenase; hexokinase; glucose; cattle; developmental stages; morula; reverse transcriptase polymerase chain reaction; blastocyst; gene expression; genes; actin; oocytes
- ... Glucose metabolism of the bovine embryo is low during the first cleavages and increases sharply after the major resumption of the genome (8-16 cells). The mRNA level for genes involved in glucose metabolism was tested by RT-PCR on individual oocytes and embryos at different stages of development. These genes were: glucose transport GLUT-1, hexokinase (HK), glucose-6-phosphate-dehydrogenase (G6PDH) ...
- Shuang Liang; Ming-Hui Zhao; Jing Guo; Jeong-woo Choi; Nam-Hyung Kim; Xiang-Shun Cui
- Theriogenology 2016 v.85 no.4 pp. 754-761.e1
- actin; cattle; centrioles; chromosomes; cytokinesis; developmental stages; double-stranded RNA; early development; embryogenesis; fluorescence; fluorescent antibody technique; gene expression; meiosis; messenger RNA; metaphase; oocytes; staining
- ... PLK4, a polo-like kinase (PLK) family member that accumulates in the cytoplasm, has been identified as a crucial regulator of centriole formation. PLK4 also controls several essential cellular functions, including cytokinesis and gene expression. In this study, we investigated the expression and function of PLK4 during bovine oocyte meiotic maturation and subsequent embryo development. The PLK4 mR ...