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You searched for: bovin Remove constraint bovin Subject term developmental stages Remove constraint Subject term: developmental stages
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1. 1 Whole-genome bisulfite sequencing of bovine gametes and invivo-produced pre-implantation embryos

Author:
J. E. Duan; Z. Jiang; F. Alqahtani; I. Mandoiu; H. Dong; X. Zheng; S. L. Marjani; J. Chen; X. C. Tian
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 126
ISSN:
1031-3613
Subject:
DNA demethylation; DNA libraries; DNA methylation; Holstein; X chromosome; bisulfites; bulls; cows; developmental stages; embryo (animal); embryogenesis; epigenetics; gene expression; genome; genomics; humans; in vitro fertilization; intergenic DNA; mitochondrial DNA; models; oocytes; sequence analysis; spermatozoa
Abstract:
... Dynamic changes in DNA methylation are crucial in the epigenetic regulation of mammalian embryogenesis. Global DNA methylation studies in the bovine, however, remain mostly at the immunostaining level. We adopted the single-cell whole-genome bisulfite sequencing method to characterise stage-specific genome-wide DNA methylation in bovine sperm, individual oocytes derived invivo and invitro, and inv ...
DOI:
10.1071/RDv31n1Ab1

2. 161 effect of oviductal fluid on bovine oocyte zona pellucida hardening and sperm binding, and on early embryo development

Author:
P. Hugon; J. Lamy; E. Corbin; P. Mermillod; M. Saint-Dizier
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 210
ISSN:
1031-3613
Subject:
additives; analysis of variance; blastocyst; corpus luteum; cows; cytoplasm; developmental stages; digestion; embryo culture; embryogenesis; ethanol; fluorescence microscopy; in vitro fertilization; oocytes; oviducts; ovulation; proteinases; slaughterhouses; spermatozoa; zona pellucida
Abstract:
... This study was designed to evaluate the effects of oviductal fluid at different periovulatory times on oocyte maturation, modification of the zona pellucida (ZP), fertilization and embryo development. Bovine oviducts were collected at a slaughterhouse and classified as preovulatory (pre-ov: 1 pre-ov follicle and a regressing corpus luteum) or post-ovulatory (post-ov: a corpus haemorrhagicum or rec ...
DOI:
10.1071/RDv28n2Ab160

3. 2 Role of histone H3 lysine 9 trimethylation during bovine pre-implantation embryonic development

Author:
M. Navarro; C. Bluguermann; M. Von Meyeren; V. Bariani; C. Osycka; A. Mutto
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 126
ISSN:
1031-3613
Subject:
DNA; analysis of variance; blastocyst; cattle; cytoplasm; data collection; developmental stages; embryogenesis; gene editing; gene silencing; genes; germinal vesicle; heterochromatin; histones; in vitro fertilization; luciferase; lysine; messenger RNA; metaphase; methylation; methyltransferases; quantitative polymerase chain reaction; somatic cells; zygote
Abstract:
... Histones play an important role in DNA’s compaction and organisation into the cellular nucleus. Depending on which histone modification occurs, chromatin can take a conformation of heterochromatin or euchromatin, which are associated with gene repression or expression, respectively. Histone H3 lysine 9 (H3K9) trimethylation (H3K9me3) is associated with gene silencing. At least 3 methyltransferases ...
DOI:
10.1071/RDv31n1Ab2

4. 21 The contrasting role of histone methyltransferases during nuclear reprogramming: SUV39H knockdown improves bovine somatic cell nuclear transfer, while the absence of EHMT2 hampers it

Author:
R. Sampaio; D. Ambrizi; R. Nociti; J. Pinzon; J. Sangalli; T. De Bem; K. Takahashi; J. Cruz; L. Smith; P. Ross; F. Meirelles
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 136
ISSN:
1031-3613
Subject:
biological control; blastocyst; catalytic activity; cattle; developmental stages; embryogenesis; epigenetics; fibroblasts; genes; histones; in vitro fertilization; methylation; methyltransferases; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; small interfering RNA; somatic cells
Abstract:
... The persistence of somatic epigenetic memory is indicated as the main barrier for efficient nuclear reprogramming. The augmented levels of histone methylation on somatic nuclei have been shown as the major regulator of this aberrant remodelling. Although they occupy the same site, H3K9me2 and H3K9me3 are catalysed by different histone methyltransferases (HMTs), EHMT2 and SUV39H, respectively. Howe ...
DOI:
10.1071/RDv32n2Ab21

5. 31 Effect of vitrification on global gene expression dynamics of bovine elongating embryos

Author:
Z. Jiang; E. Gutierrez; H. Ming; B. Foster; L. Gatenby; C. Mak; C. Pinto; K. Bondioli
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 141
ISSN:
1031-3613
Subject:
DNA repair; adherens junctions; cooling; cows; cryopreservation; cryoprotectants; developmental stages; dimethyl sulfoxide; embryo (animal); embryogenesis; energy; epithelium; ethylene glycol; gene expression; gene expression regulation; gene ontology; genes; germ cells; liquid nitrogen; messenger RNA; mitochondria; osmotic stress; oxidative phosphorylation; rapamycin; sequence analysis; straw; transcriptome; ubiquitination; vitrification
Abstract:
... The ability to cryopreserve gametes and embryos has been a valuable tool for reproductive management in all mammalian species, especially livestock. Embryo vitrification involves exposure to high concentrations of cryoprotectants and osmotic stress during cooling and warming. These factors have to affect gene expression. The elongating embryo is a stage of embryo development that can be recovered ...
DOI:
10.1071/RDv32n2Ab31

6. 49 Expression and actions of the dickkopf-1 receptors KREMEN1 and KREMEN2 in the bovine pre-implantation embryo

Author:
T. Fernandes Amaral; Y. Xiao; E. Estrada-Cortes; P. Hansen
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 149-150
ISSN:
1031-3613
Subject:
analysis of variance; blastocyst; cattle; developmental stages; embryogenesis; endometrium; essential genes; fluorescence; insemination; oligonucleotides; oocytes; pregnancy; quantitative polymerase chain reaction; receptors; reverse transcriptase polymerase chain reaction; zygote
Abstract:
... Dickkopf-1 (DKK1) is a secreted inhibitor of canonical WNT signaling expressed in the endometrium that can increase the competence of bovine blastocysts to establish pregnancy after transfer into recipients. The mechanism by which DKK1 regulates embryo function is not known. KREMEN1 and KREMEN2 are paralogs that function as DKK1 transmembrane receptors. Binding of DKK1 to KREMEN leads to internali ...
DOI:
10.1071/RDv32n2Ab49

7. 56 Mammalian pre-implantation embryos at the single cell level: The bovine as a model for early human embryonic development

Author:
N. Ortega; V. Ahola; A. Plaza-Reyes; J. Schell; P. Kumar; A. Jouneau; V. Duranthon; F. Lanner
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 153
ISSN:
1031-3613
Subject:
animal models; antagonism; blastocyst; blastomeres; cattle; developmental stages; early development; embryogenesis; embryonic stem cells; endoderm; ethics; gene editing; gene expression regulation; genes; humans; mice; sequence analysis; signal transduction; transcriptomics
Abstract:
... Single-cell transcriptomics and gene editing on human pre-implantation embryos have proved that mechanisms previously identified and well characterised in mouse pre-implantation development may not hold true in the human embryo. However, ethical and legal concerns limit the availability of surplus human embryos for research, resulting in the need for novel animal models. Bovine embryos share morph ...
DOI:
10.1071/RDv32n2Ab56

8. 57 Proteomic analysis reveals metabolic dysregulation in invitro-cultured bovine embryos

Author:
S. K. Rajput; H. Fernandes; R. Kile; R. C. West; D. M. Logsdon; B. Goheen; Y. Yuan; W. B. Schoolcraft; R. L. Krisher
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 135
ISSN:
1031-3613
Subject:
Western blotting; acetyl coenzyme A; beta oxidation; biomarkers; blastocyst; cattle; developmental stages; energy; gene expression regulation; glucose; glycolysis; hexokinase; in vitro culture; lactic acid; oxidative stress; pentose phosphate cycle; protein content; proteomics; pyruvic acid; superovulation; t-test; tricarboxylic acid cycle
Abstract:
... Invitro culture (IVC) systems fail to completely recapitulate the invivo environment, resulting in metabolic stress during pre-implantation development and reduced blastocyst quality. We hypothesised that IVC-induced metabolic dysregulation in bovine embryos is mediated by changes in expression and/or activity of protein biomarkers associated with key metabolic pathways. Our objectives were to det ...
DOI:
10.1071/RDv33n2Ab57

9. 59 Selected Reaction Monitoring-Based Absolute Quantification of Developmentally Relevant Proteins in Early Bovine Embryos Reveals Differences Between In Vitro and In Vivo Embryo Culture and Between Different Maternal Metabolic Stages

Author:
G. J. Arnold; K. Gegenfurtner; T. Frohlich; D. R. Deutsch; P. Salvetti; N. Forde; P. Lonergan; U. Besenfelder; E. Wolf
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 168-169
ISSN:
1031-3613
Subject:
European Union; blastocyst; cattle; developmental stages; embryo culture; embryogenesis; genome; germinal vesicle; liquids; mass spectrometry; monitoring; oogenesis; oviducts; peptides; principal component analysis; protein content; proteins; proteome; transcription (genetics); translation (genetics); zygote
Abstract:
... Early embryogenesis is a highly complex developmental process, accompanied by a plethora of changes at the morphological and molecular level. Particularly at the level of proteins, these changes are still poorly characterised and understood. During the first cleavages, the embryo depends mainly on maternal transcripts and proteins that were accumulated and stored during oogenesis until embryonic g ...
DOI:
10.1071/RDv30n1Ab58

10. 95 role of polyamines in bovine pre-implantation development

Author:
J. Herrick; A. Greene; W. Schoolcraft; R. Krisher
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 177
ISSN:
1031-3613
Subject:
EDTA (chelating agent); adverse effects; albumins; analysis of variance; animal ovaries; blastocyst; caffeine; cattle; centrifugation; citrates; culture media; cysteamine; cysteine; developmental stages; embryogenesis; essential amino acids; follicle-stimulating hormone; fructose; glucose; hatching; heparin; humans; myo-inositol; polyamines; putrescine; pyruvic acid; slaughterhouses; spermatozoa; zygote
Abstract:
... Polyamines are involved in trophectoderm attachment and outgrowth, but little is known about their role in earlier stages of development. The objective of this study was to evaluate the effects of an inhibitor of polyamine synthesis (difluoromethylornithine, DFMO) on development (blastocyst formation and hatching) and cell allocation to the trophectoderm (TE, CDX2-positive) and inner cell mass (IC ...
DOI:
10.1071/RDv28n2Ab94

11. Administration of recombinant bovine somatotropin (rbST) at the time of breeding in superovulated fertile and subfertile ewes

Author:
O. Mejía; M. Palma-Irizarry; J. Rosas; V. Madrid-Marina; M.J. Valencia; L. Zarco
Source:
Small ruminant research 2012 v.102 no.1 pp. 51-56
ISSN:
0921-4488
Subject:
blastocyst; breeding; developmental stages; embryogenesis; ewes; ovulation; progesterone; somatotropin
Abstract:
... Two experiments were conducted to evaluate the effects of the administration of 125mg rbST at the time of breeding on fertile and subfertile superovulated ewes – in terms of the ovulation rate, embryo yield and embryonic development. In addition, the effects of the treatment on the plasma progesterone concentrations and interferon-tau (IFNT) expression in the embryos were evaluated in the fertile ...
DOI:
10.1016/j.smallrumres.2011.09.016

12. Analysis of the expression of putatively imprinted genes in bovine peri-implantation embryos

Author:
Tveden-Nyborg, P.Y.; Alexopoulos, N.I.; Cooney, M.A.; French, A.J.; Tecirlioglu, R.T.; Holland, M.K.; Thomsen, P.D.; D'Cruz, N.T.
Source:
Theriogenology 2008 v.70 no.7 pp. 1119-1128
ISSN:
0093-691X
Subject:
cattle; food animals; embryo (animal); embryo implantation; genomic imprinting; gene expression; gene expression regulation; DNA methylation; developmental stages; morbidity; genetic disorders; embryonic mortality; temporal variation; parthenogenesis; in vitro fertilization; transcription (genetics)
Abstract:
... The application of assisted reproductive technologies (ART) has been shown to induce changes in the methylation of the embryonic genome, leading to aberrant gene expression, including that of imprinted genes. Aberrant methylation and gene expression has been linked to the large offspring syndrome (LOS) in bovine embryos resulting in increased embryonic morbidity and mortality. In the bovine, limit ...
DOI:
10.1016/j.theriogenology.2008.06.033
PubMed:
18675451

13. Biopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates

Author:
Regivaldo Vieira de Sousa; Célia Regina da Silva Cardoso; Guilberth Butzke; Margot Alves Nunes Dode; Rodolfo Rumpf; Maurício Machaim Franco
Source:
Theriogenology 2017 v.90 pp. 25-31
ISSN:
0093-691X
Subject:
DNA; animal breeding; assisted reproductive technologies; biopsy; cattle; developmental stages; genomics; marker-assisted selection; polymerase chain reaction; pregnancy rate; viability
Abstract:
... Assisted reproductive techniques have significantly contributed to animal breeding programs. Similarly, genomics has provided important information and tools to improve the accuracy of selection. However, the greatest benefits of those tools can only be expected when they are combined, allowing animals to be selected accurately early in life. Therefore, obtaining DNA samples from embryos without c ...
DOI:
10.1016/j.theriogenology.2016.11.003

14. Cellular Composition and Viability of Cloned Bovine Embryos Using Exogene-Transfected Somatic Cells

Author:
Lee, S-L.; Kumar, BM; Kim, J-G.; Ock, S-A.; Jeon, B-G.; Balasubramanian, S.; Choe, S-Y.; Rho, G-J.
Source:
Reproduction in domestic animals 2007 v.42 no.1 pp. 44-52
ISSN:
0936-6768
Subject:
apoptosis; blastocyst; cattle; developmental stages; diploidy; fluorescence microscopy; gene expression; genes; in vitro fertilization; polymerase chain reaction; somatic cells; viability
Abstract:
... The present study compared the efficiency of transgenic (TG) cloned embryo production by somatic cell nuclear transfer (SCNT) with fetal-derived fibroblast cells (FFCs) which were transfected with pEGFP-N1 to in vitro-fertilized (IVF), parthenogenetic and SCNT counterparts by evaluating the rates of cleavage and blastocyst formation, apoptosis rate at different developmental stages, cell number, p ...
DOI:
10.1111/j.1439-0531.2006.00723.x
PubMed:
17214773

15. Characterization and comparison of whey N-glycoproteomes from human and bovine colostrum and mature milk

Author:
Xueyan Cao; Mei Yang; Ning Yang; Xiaona Liang; Dongbing Tao; Biao Liu; Junrui Wu; Xiqing Yue
Source:
Food chemistry 2019 v.276 pp. 266-273
ISSN:
0308-8146
Subject:
cow colostrum; developmental stages; gene ontology; glycoproteins; human colostrum; humans; infant formulas; lactation; lectins; liquid chromatography; milk; nutrients; tandem mass spectrometry; whey
Abstract:
... Milk glycoproteins are crucial nutrients with a variety of functions. However, whey N-glycoproteomes in human and bovine milks have not been characterized during lactation. Herein, using lectin enrichment and liquid chromatography tandem mass spectrometry, 68, 58, 100, and 98 N-glycoproteins were identified in human colostrum and mature milk as well as bovine colostrum and mature milk whey. Gene O ...
DOI:
10.1016/j.foodchem.2018.09.174

16. Compositional and functional dynamics of the bovine rumen methanogenic community across different developmental stages

Author:
Nir Friedman; Elie Jami; Itzhak Mizrahi
Source:
Environmental microbiology 2017 v.19 no.8 pp. 3365-3373
ISSN:
1462-2912
Subject:
Methanosarcinales; carbon; cattle; developmental stages; ecosystems; food webs; methane; methane production; methanogens; microbiome; rumen; rumen fermentation; taxonomy
Abstract:
... Methanogenic archaea in the bovine rumen are responsible for the reduction of carbon molecules to methane, using various electron donors and driving the electron flow across the microbial food webs. Thus, methanogens play a key role in sustaining rumen metabolism and function. Research of rumen methanogenic archaea typically focuses on their composition and function in mature animals, while studie ...
DOI:
10.1111/1462-2920.13846
PubMed:
28654196
PubMed Central:
PMC6488025

17. Correlation between the cell number and diameter in bovine embryos produced in vitro

Author:
Mori, M.; Otoi, T.; Suzuki, T.
Source:
Reproduction in domestic animals 2002 v.37 no.3 pp. 181-184
ISSN:
0936-6768
Subject:
diameter; cattle; developmental stages; age; cells; blastocyst; embryogenesis; embryo culture
DOI:
10.1046/j.1439-0531.2002.00354.x
PubMed:
12071894

18. Cryopreservation of in vitro produced bovine embryos: effects of lipid segregation and post-thaw laser assisted hatching

Author:
J.H. Pryor; C.R. Looney; S. Romo; D.C. Kraemer; C.R. Long
Source:
Theriogenology 2011 v.75 no.1 pp. 24-33
ISSN:
0093-691X
Subject:
cattle; centrifugation; cryopreservation; cytochalasin B; developmental stages; ethylene glycol; freezing; hatching; in vitro fertilization; morula; zona pellucida
Abstract:
... The objective was to determine if lipid segregation, with or without post-thaw laser assisted hatching (LAH) of in vitro produced (IVP) bovine embryos, would enhance in vitro survivability and development 24 h post-thaw. On Day 6 of culture (Day 0 = IVF), in vitro produced bovine embryos were divided into three developmental stages: 32-cell (n = 78), compact morula (CM n = 223), and blastocyst (n ...
DOI:
10.1016/j.theriogenology.2010.07.006
PubMed:
20833420

19. Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence [Erratum: 2010 May, v. 73, no. 8, p. 1164]

Author:
Ferreira, E.M.; Vireque, A.A.; Adona, P.R.; Meirelles, F.V.; Ferriani, R.A.; Navarro, P.A.A.S.
Source:
Theriogenology 2009 v.71 no.5 pp. 836-848
ISSN:
0093-691X
Subject:
transcription factors; messenger RNA; animal proteins; cattle; developmental stages; chromosome segregation; ribosomes; mitochondria; microtubules; cytochemistry; sarcoplasmic reticulum; ultrastructure; oocytes; Golgi apparatus
Abstract:
... Oocyte maturation is a long process during which oocytes acquire their intrinsic ability to support the subsequent stages of development in a stepwise manner, ultimately reaching activation of the embryonic genome. This process involves complex and distinct, although linked, events of nuclear and cytoplasmic maturation. Nuclear maturation mainly involves chromosomal segregation, whereas cytoplasmi ...
DOI:
10.1016/j.theriogenology.2008.10.023

20. Development of bovine embryos in single in vitro production (sIVP) systems

Author:
Hagemann, L.J.; Weilert, L.L.; Beaumont, S.E.; Tervit, H.R.
Source:
Gamete research 1998 v.51 no.2 pp. 143-147
ISSN:
1040-452X
Subject:
oocytes; cattle; developmental stages; embryo (animal); fertilization (reproduction); embryogenesis; embryo culture; culture media
DOI:
10.1002/(SICI)1098-2795(199810)51:2<143::AID-MRD3>3.0.CO;2-Q
PubMed:
9740321