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... Plant chemicals in three cruciferous crop species, Brassica napus L., B. juncea (L.) Czerniak, and Sinapis alba L., that stimulate oviposition in the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) were investigated in laboratory bioassays. Aerial portions of 4- to 6-week-old plants were extracted and fractionated using ion-exchange liquid chromatography. The oviposition stim ...
Brassica napus; Brassica oleracea var. botrytis; stomatal movement; zinc; dose response; nutrient deficiencies; leaves; waxes; biosynthesis; proline; carbonate dehydratase; enzyme activity; leaf water potential; guard cells; potassium; chemical constituents of plants; thickness
Abstract:
... Zn deficiency induced increases in epicuticular wax deposits, lamina thickness, degree of succulence, water saturation deficit, diffusive resistance, and proline accumulation and decreases in carbonic anhydrase activity, water potential, stomatal aperture, and transpiration in the leaves of cauliflower (Brassica oleracea L. var botrytis cv Pusa) plants. Restoration of Zn supply to the deficient pl ...
... We have examined the effects of stereochemically pure analogs of abscisic acid (ABA) on three responses in Brassica napus microspore embryos. The analogs used include modifications to natural (S-) (+)-ABA (=N-ABA) at the C-1 and C-1' positions. At the C-1 position, the carboxylic acid function was replaced with an alcohol, aldehyde, or methyl ester functional group, and at the chiral C-1' position ...
soil fungi; volatile compounds; cultivars; dose response; plant diseases and disorders; cultural control; Brassica nigra; chemical constituents of plants; Brassica rapa subsp. oleifera; plant extracts; green manures; Brassica juncea; Brassica carinata; disease control; plant pathogenic fungi; Brassica napus; genetic variation; Fusarium sambucinum; soil fumigation; Sinapis alba subsp. alba
Abstract:
... Brassica species were tested for production of volatile fungicidal compounds from macerated green leaf tissue. Tissue (10, 20, and 40 g) of one cultivar each of six Brassica species was assayed for inhibition of Fusarium sambucinum. Only cultivars of B. nigra and B. juncea suppressed radial growth (>50% inhibition of control). Plant introduction accessions (PIs) of B. juncea, B. carinata, B. nigra ...
... Hairy roots cultures derived from leaf explants of Brassica napus L. produced and secreted peroxidases. The enzyme activity in the medium increased with growth but it remained nearly constant in the tissue. The changes in extracellular peroxidase activity seemed to be correlated with the increase in a basic peroxidase of pI: 9.6. Four isoenzymes with pI in the range 8.5-9.6 and a neutral peroxidas ...
... Internode segments from aseptic shoot cultures are the most prolific explants for the regeneration of Brassica shoots in vitro. These explants also have the advantage of not being subject to the genotypic variations in regeneration response observed in hypocotyl and cotyledon explants. Despite reports of 80-100% shoot regeneration from stem explants, observed frequencies are typically 50-60%. Thre ...
... A study on the physiological and yield effects of waterlogging and the alleviation of waterlogging damage by the application of nitrogen fertilizers and mixtalol in winter rape was conducted in experimental tanks especially designed for controlling soil moisture content. The results showed that waterlogging at the seedling and stem elongation stages causes a significant decrease in nitrogen conten ...
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
... Brassica tissues are potentially useful in the control of Aphanomyces root rot of peas (Pisum sativum), but identity of the responsible compounds and specific impacts of those compounds on the pathogen's infection potential remain uncertain. Brassica napus seed meals and water extracts from these meals were used to determine the effect of glucosinolate hydrolysis products on Aphanomyces euteiches ...
... Biochemical changes in leaves of 3-week-old oil seed rape (Brassica napus L. cv. Iris) plants were determined shortly after treatment with different doses of glyphosate. Secondary effects of N-(phosphonomethyl)glycine (glyphosate) on activity of the enzyme phenylalamine ammonia-lyase was not related to dose of glyphosate by any known model within the first 48 h after spraying. Accumulation of shik ...
... Isolated microspores of Brassica napus were cultured on high concentrations of mannitol or polyethylene glycol (PEG 4000), with only a very limited amount of sucrose (0.08-0.1%) provided as carbohydrate source in the medium. While microspores cultured on high mannitol yielded no embryos and no embryogenic cell divisions were observed, microspores on high PEG developed into embryos within 2 weeks, ...
... A simple method to induce somatic embryogenesis from seeds of rapid-cycling Brassica napus is described. Seedlings cultured on Murashige and Skoog (MS) basal medium produced somatic embryos directly on hypocotyls and cotyledons after 2 to 3 subcultures onto the same medium. A low pH of the medium (3.5-5) was more conducive to somatic embryogenesis than a higher pH (6 and 7). Embryogenic potential ...
... A cell suspension culture assay to determine the phytotoxicity of the fungal toxins phomalide, a host-selective toxin produced by the fungus Phoma lingam (Tode ex Fr.) Desm., perfect stage Leptosphaeria maculans (Desm.) Ces. et de Not., and destruxin B, the major host-selective toxin produced by the fungus Alternaria brassicae (Berk.) Sacc., was carried out with three Brassica spp. It was establis ...
... The availability of doubled haploids could greatly contribute to improving seed quality in condiment mustard (Brassica juncea). We have developed an efficient and reliable protocol of microspore culture, modified from that of Baillie et al. (1992), based on a modification of the sucrose concentration of culture media. A comparison of microspore culture media differing in their sucrose content show ...
... We investigated the effects of plant density on plant size, leaf total soluble protein content, and constitutive and wound-induced levels of proteinaceous trypsin inhibitors in pot-grown Brassica napus seedlings in two greenhouse studies. We manipulated plant density by varying the number of intraspecific neighbors surrounding a target plant in the center of each pot. In general, constitutive and ...
... The effect of colchicine on embryogenesis induction and chromosome doubling during microspore culture was evaluated in two F1 hybrids of winter oilseed rape (Brassica napus L.). Colchicine treatment (50 and 500 mg/L) of isolated microspores during the first 15 h in culture stimulated embryogenesis and produced large amounts of healthy-looking embryos. These normal embryos germinated well at 24 deg ...
... Five different varieties of Brassica napus (Cyclone, Dunkled, Oscar, Rainbow and KS75) were tested for their regeneration response. Cyclone showed a very high frequency of regeneration (92%). The use of silver nitrate was a pre-requisite for efficient shoot regeneration. Hypocotyls were selected as the starting material for transformation experiments on the basis of high transient GUS expression. ...
... Efficient and simple protocols were developed for conversion of embryos derived from microspores in rape. The frequency of embryo conversion was higher than 80% on improved media without pretreatments using ABA, GA₃ or desiccation, which had been required before embryos were transferred to solid media for conversion. While on basal media such as 1/2MS, the conversion frequency was no more than 32% ...
... A laser beam directed towards weeds can be an efficient weed control method as an alternative to herbicides. Lasers may deliver high-density energy to selected plant material, raising the temperature of the water in the plant cells and thereby stop or delay the growth. A commercial use of lasers for weed control, however, require a systematic investigation of the relationship between energy densit ...
... The effects of nitrogen fertiliser on herbicide dose-response of weeds were investigated by measuring weed biomass after growth at a range of nitrogen levels and treatment with a range of herbicide doses. Increasing weed biomass at no-herbicide treatment (W₀) and the response rate of the dose-response curve (B), with increasing nitrogen were successfully described by the linear model and the expon ...
... Dimethyl disulfide (DMDS) was identified as a major volatile constituent of Brassica napus roots heavily infested by Delia radicum, the cabbage root fly. Attractiveness of this widespread compound was tested in the field in a naturally complex odorous environment. By using an original setup especially designed for ground dwelling beetles, different concentrations of the pure molecule as well as at ...
... Sclerotinia sclerotiorum (Lib.) de Bary is a phytopathogenic fungus capable of causing significant yield losses in numerous crops, including canola, in which the fungus causes sclerotinia stem rot. Immunological detection methods to rapidly determine the presence of S. sclerotiorum on plants may provide growers with a viable diagnostic tool to aid with fungicide use decisions. This paper discusses ...
... Canola is one of the most important oilseed crops, and its seed yield and quality are significantly affected by environmental stresses such as drought. The phytohormone abscisic acid (ABA) is induced by drought and triggers stomatal closure to reduce transpiration, which accounts for >90% of water loss in plants. The ABA-mediated stomatal response is a dosage-dependent process that can be achieved ...
... The risk that insect-resistant transgenic plants may pose for solitary bees was assessed by determining longevity of adult Osmia bicornis (O. rufa) chronically exposed to transgenic oilseed rape expressing oryzacystatin-1 (OC-1) or to the purified insecticidal proteins recombinant rOC-1, Kunitz soybean trypsin inhibitor (SBTI), Galanthus nivalis agglutinin (GNA), or Bacillus thuringiensis toxin Cr ...
... A full-length cDNA encoding a putative G protein β subunit (Gβ), designated as BnGB1, was isolated from Brassica napus. BnGB1 was predicted to encode a precursor protein of 378-amino acid residues. The expression of BnGB1 in different B. napus tissues and developmental stages was analyzed using real-time polymerase chain reaction. The results showed that BnGB1 expressed was high at the seventh day ...
Brassica napus; flowers; plant secretions and exudates; signal transduction; jasmonic acid; gibberellic acid; volatile compounds; dose response
Abstract:
... Nectar is a rich source of sugars that serves the attraction of pollinators (floral nectar) or predatory arthropods (extrafloral nectar). We just begin to understand the similarities and differences that underlie the secretory control of these two important types of plant secretions. Jasmonates are phytohormones, which are well documented to be involved in plant developmental processes and plant d ...
... Tropospheric ozone is an important air pollutant with known detrimental effects for several crops. Ozone effects on seed yield, oil percentage, oil yield and 1000 seed weight were examined for spring oilseed rape (Brassica napus cv. Ability). For broccoli (Brassica oleracea L. cv. Italica cv. Monaco) the effects on fresh marketable weight and total dry weight were studied. Current ozone levels wer ...
... Plant seed oil represents a major renewable source of reduced carbon, but little is known about the biochemical regulation of its synthesis. The goal of this research was to identify potential feedback regulation of fatty acid biosynthesis in Brassica napus embryo-derived cell cultures and to characterize both the feedback signals and enzymatic targets of the inhibition. Fatty acids delivered via ...
... Pollen beetle, Meligethes aeneus (Fabricius) (Coleoptera: Nitidulidae), and cabbage seed weevil, Ceutorhynchus assimilis (Paykull) (Coleoptera: Curculionidae), are important pests in the production of European winter oilseed rape, Brassica napus L. (Brassicaceae), which is grown on several million hectares in Europe. Insecticide treatments are common to control both pests once they exceed economic ...
... Phytoextraction is an eco-friendly and cost-effective technique for removal of toxins, especially heavy metals and metalloids from contaminated soils by the roots of high biomass producing plant species with subsequent transport to aerial parts. Lower metal bioavailability often limits the phytoextraction. Organic chelators can help to improve this biological technique by increasing metal solubili ...
Brassica napus; canola; cole crops; cultivars; data analysis; dose response; field experimentation; grain yield; irrigation; plant growth; potassium; proline; sugar content; water content; water requirement
Abstract:
... One of the major issues with brassica oil seed production is the water requirement of the brassica crop. To address the problem, field experiments were conducted to evaluate the effect of potassium (K) and water deficiency levels on canola (Brassica napus L.). Analysis of the data revealed that application of K, irrigation and interactions between irrigation and cultivar (I x C), irrigation and po ...
Brassica napus; Lolium rigidum; at-risk population; atrazine; canola; clethodim; dose response; field experimentation; genes; mutation; plant density; propyzamide; seed yield; split application; weed control; South Australia
Abstract:
... Two field experiments were conducted during 2013 and 2014 at Roseworthy, South Australia, to identify effective herbicide options for the control of clethodim-resistant Lolium rigidum in triazine-tolerant canola. Dose response experiments in pots confirmed resistance in both field populations (F4-2013 and S2-2014) of L. rigidum to clethodim and butroxydim. At the recommended field rate of clethodi ...
... Alopecurus aequalis, a predominant weed species in wheat and oilseed rape fields, can no longer be controlled by mesosulfuron-methyl application after continuous use over several years. Based on dose–response studies, the putative resistant populations, JTJY-1 and JHHZ-1, were found to be resistant to mesosulfuron-methyl, with resistance index values of 5.5 and 14, respectively. Sensitivity assays ...
... Shortawn foxtail (Alopecurus aequalis) is a troublesome grass weed infesting winter wheat and oilseed rape productions in China. Fenoxaprop-p-ethyl and mesosulfuron-methyl failed to control shortawn foxtail of AHSX-1 population collected from a wheat field in Shou County, Anhui province. Molecular analyses revealed that Asp2078Gly mutation of ACCase and Trp574Leu mutation of AHAS were present in p ...
... Japanese foxtail is a predominant tetraploid grass weed in wheat and oilseed rape fields in eastern China. In China, pyroxsulam is mainly used to manage annual grass weeds, especially those resistant to acetyl coenzyme A carboxylase (ACCase)-inhibiting herbicides. Using dose–response studies, a pyroxsulam-resistant population, ACTC-1, was identified with a resistance index value of 58. Additionall ...
... Fiber hemp plants (Cannabis sativa L.) display competitive and allelopathic activity against weeds. Fiber hemp essential oil (EO) was previously assessed for its antimicrobial properties. In this work we aimed to assess the allelopathic efficacy of this EO. The essential oil derived from the inflorescences of fiber hemp cv. ‘Bialobrzeskie’ contained mainly mono- and sesquiterpene hydrocarbons, as ...
... Salicylic acid (SA) mediates tolerance mechanisms in plants against a wide spectrum of biotic and abiotic stresses. Therefore, the present study was carried out to determine how SA regulates the plant protection mechanisms in two cultivars of oilseed rape (Brassica napus L.) under chromium (Cr) stress. Exogenously applied SA enhanced plant growth, increased dry biomasses, and strengthened the reac ...
... We investigated the specific features of copper and zinc excess action on the roots of canola (Brassica napus L.) plants. Copper rapidly accumulated in canola root cells and reached saturation during several hours of treatment, whereas the root zinc content increased relatively slowly. Excessive copper and zinc entry inside the cell resulted in significant cell damage, as evidenced by alterations ...
... The duration of the soil activity of an acetolactate synthase-(ALS) inhibiting herbicide which is currently under approval for sugar beet cultivation was determined in a field trial series in Germany in 2013 and 2014. The herbicide containing foramsulfuron (FSN; 50 g L⁻¹) and thiencarbazone-methyl (TCM; 30 g L⁻¹) was applied in different dosages (25 + 15, 37.5 + 22.5 and 50 + 30 g FSN+TCM ha⁻¹) to ...
... Polyploidy, the possession of multiple sets of chromosomes, has been a predominant factor in the evolution and success of the angiosperms. Although artificially formed allopolyploids show a high rate of genome rearrangement, the genomes of cultivars and germplasm used for crop breeding were assumed stable and genome structural variation under the artificial selection process of commercial breeding ...
... This work aimed to assess the phytotoxic potential of 12 essential oils (EOs) collected from plants growing in natural or cultivated stands in a temperate climate, i.e., Achillea millefolium, Acorus calamus, Carum carvi, Chamomilla recutita, Foeniculum vulgare, Lavandula angustifolia, Melissa officinalis, Mentha × piperita, Salvia officinalis, Solidago canadensis, Tanacetum vulgare and Thymus vulg ...
Anabaena; Brassica napus; arsenic; beta-glucosidase; bioassays; cement; copper; dose response; ecotoxicology; enzyme activity; iron; iron oxides; luminescence; median effective concentration; mining; pH; plant growth; planting; polluted soils; pollution; pulp and paper sludge; rolling; seedlings; seeds; sulfates; toxicity; zinc
Abstract:
... Abandoned waste piles from ancient mining activities are potential hot spots for the pollution of the surrounding areas. A pot experiment was carried out to check the potential toxicity of the dumping material present in one of these scenarios, and several amendments were tested to attenuate the spread of the contamination events. The waste material had an acid pH and a large total concentration o ...
Arabidopsis thaliana; Brassica napus; abscisic acid; cysteine; dose response; genome; glutathione; mass spectrometry; oxidation; protein kinases; protein phosphorylation; reducing agents; stomatal movement; sucrose; sulfonic acids; thiols; transcription (genetics); tyrosine
Abstract:
... Sucrose nonfermenting 1‐related protein kinase 2.6 (SnRK2.6), also known as Open Stomata 1 (OST1) in Arabidopsis thaliana, plays a pivotal role in abscisic acid (ABA)‐mediated stomatal closure. Four SnRK2.6 paralogs were identified in the Brassica napus genome in our previous work. Here we studied one of the paralogs, BnSnRK2.6‐2C, which was transcriptionally induced by ABA in guard cells. Recombi ...
... To investigate the physiological and biochemical mechanisms of nitric oxide (NO)-induced paraquat (PQ) tolerance in plants, we pretreated a set of 10-day-old Brassica napus seedlings with 500 μM sodium nitroprusside (SNP – a NO donor) for 24 h. Then, three doses of PQ (62.5, 125 and 250 μM) were applied separately, as well as to SNP-pretreated seedlings, and the seedlings were allowed to grow for ...
... Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant growth regulator through the anti-gibberellin activity. And notably, the reduction of Brassica napus L. growth can be attributed to the inhibition of gibberellin biosynthesis at the stage of conversion ...