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- Author:
- Vergauwe, A.; Cammaert, R.; Vandenberghe, D.; Genetello, C.; Inze, D.; Montagu, M. van.; Eeckhout, E. van den.
- Source:
- Plant cell reports 1996 v.15 no.12 pp. 929-933
- ISSN:
- 0721-7714
- Subject:
- benzyladenine; micropropagation; neomycin; 2,4-D; callus; shoots; dose response; Agrobacterium radiobacter; explants; naphthaleneacetic acid; leaves; polymerase chain reaction; Artemisia annua; rooting; phosphotransferases (phosphomutases); enzyme activity; culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; nucleotide sequences; beta-glucuronidase
- Abstract:
- ... A transformation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphthaleneacetic acid and 0.5 mg/L N6-benzyladenine after transformation with the C58C1 Rif(R) (pGV2260)(pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Ka ...
- DOI:
- 10.1007/BF00231590
- PubMed:
- 24178277
- https://doi.org/10.1007/BF00231590
- Author:
- Blay, E.; Oakes, J.V.
- Source:
- Plant cell reports 1996 v.15 no.8 pp. 582-585
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; dose response; kanamycin; hypocotyls; Solanum aethiopicum; beta-glucuronidase; transgenic plants; Agrobacterium radiobacter; explants; cotyledons; gene expression; histochemistry; culture media; roots; genetic markers
- Abstract:
- ... An efficient system for Agrobacterium tumefaciens-mediated transformation of Solanum gilo was established. The marker genes for kanamycin resistance and beta-glucuronidase expression were introduced. A comparison between cotyledon and hypocotyl explants showed that while regeneration was better from hypocotyl explants, cotyledon explants gave better transformation efficiency (46% vs. 32%). Four le ...
- DOI:
- 10.1007/BF00232457
- PubMed:
- 24178522
- https://doi.org/10.1007/BF00232457
- Author:
- Bondt, A. de; Eggermont, K.; penninckx, I.; Goderis, I.; Browkaert, W.F.
- Source:
- Plant cell reports 1996 v.15 no.7 pp. 549-554
- ISSN:
- 0721-7714
- Subject:
- gels; glufosinate; genetic markers; shoots; dose response; Agrobacterium radiobacter; explants; gene expression; Malus domestica; leaves; cultivars; phosphinothricin acetyltransferase; enzyme activity; culture media; genetic transformation; kanamycin; transgenic plants; zeatin; genetic variation; beta-glucuronidase
- Abstract:
- ... We have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. Jonagold) explants with Agrobacterium tumefaciens (De Bondt et al. 1994, Plant Cell Reports 13: 587-593). Now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultiva ...
- DOI:
- 10.1007/BF00232992
- PubMed:
- 24178471
- https://doi.org/10.1007/BF00232992
- Author:
- Zhou, Y.; Magill, J.M.; Magill, C.W.; Newton, R.J.
- Source:
- Plant cell reports 1996 v.15 no.11 pp. 815-818
- ISSN:
- 0721-7714
- Subject:
- Daucus carota; DNA methylation; transgenic plants; gene expression; genes; gene transfer; chimerism; beta-glucuronidase; enzyme activity; cytidine; abscisic acid; dose response; quantitative analysis
- Abstract:
- ... DNA methylation has been associated with gene activity in differentiating and developing plant tissues. The objective of this study was to determine the involvement of methylation in the expression of a gene transferred into carrot (Daucus carota L.) tissues by particle bombardment. Expression of the Dc8-GUS gene construct in response to treatments with 5-azacytidine (5-azaC) and to in vitro methy ...
- DOI:
- 10.1007/BF00233146
- PubMed:
- 24178214
- https://doi.org/10.1007/BF00233146
- Author:
- Kiyokawa, S.; Kikuchi, Y.; Kamada, H.; Harada, H.
- Source:
- Plant cell reports 1996 v.15 no.8 pp. 606-609
- ISSN:
- 0721-7714
- Subject:
- corolla; Rhizobium rhizogenes; plasmids; genes; benzyladenine; shoots; dose response; phenotype; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; leaves; Begonia tuberhybrida; enzyme activity; culture media; Begonia cucullata; genetic transformation; gene transfer; dwarfing; kanamycin; transgenic plants; Begonia hiemalis; beta-glucuronidase
- Abstract:
- ... We have developed an Agrobacterium-mediated transformation system for commercial Begonia species. The leaf explants of Begonia semperflorens, Begonia x hiemalis and B. tuberhybrida were inoculated with Agrobacterium tumefaciens LBA4404 harboring a binary vector pBI121 which contains rolA,B and C genes of an agropine type Ri plasmid (pRiA4b). Kanamycin resistant shoots of B. tuberhybrida were obtai ...
- DOI:
- 10.1007/BF00232462
- PubMed:
- 24178527
- https://doi.org/10.1007/BF00232462
- Author:
- Tzfira, T.; Ben-Meir, H.; Vainstein, A.; Altman, A.
- Source:
- Plant cell reports 1996 v.15 no.8 pp. 555-571
- ISSN:
- 0721-7714
- Subject:
- Rhizobium rhizogenes; plasmids; Populus tremula; neomycin; callus; shoots; genetic markers; dose response; stems; explants; clones; gene expression; adventitious roots; buds; genetic code; cell culture; phosphotransferases (phosphomutases); culture media; genetic transformation; kanamycin; transgenic plants; plant morphology; beta-glucuronidase
- Abstract:
- ... The natural capacity of aspen (Populus tremula L.) roots for direct shoot-bud regeneration was harnessed to establish a highly efficient transformation and regeneration procedure that does not require a pre-selection stage on antibiotics. Aspen stem segments were transformed using wildtype Agrobacterium rhizogenes (LBA9402) with the binary p35SGUSINT plasmid carrying the genes coding for beta-gluc ...
- DOI:
- 10.1007/BF00232452
- https://doi.org/10.1007/BF00232452
- Author:
- Pool-Zobel, B.L.; Neudecker, C.; Domizlaff, I.; Ji, S.; Schillinger, U.; Rumney, C.; Moretti, M.; Vilarini, I.; Scassellati-Sforzolini, R.; Rowland, I.
- Source:
- Nutrition and cancer 1996 v.26 no.3 pp. 365-380
- ISSN:
- 0163-5581
- Subject:
- genotoxicity; heat treatment; extracts; Lactobacillus acidophilus; Lactobacillus gasseri; Bifidobacterium longum; acetone; Bifidobacterium breve; glutathione; dose response; Weissella confusa; cell walls; glutathione transferase; DNA; cytoplasm; Streptococcus thermophilus; beta-glucosidase; colon; strain differences; inactivation; cytochrome P-450; N-methyl-N-nitrosoguanidine; duration; sodium chloride; rats; enzyme activity; acetic acid; liver; freeze drying; lactic acid bacteria; oxidoreductases; cultured cells; animal models; 1,2-dimethylhydrazine; beta-glucuronidase
- Abstract:
- ... Lactic acid bacteria (LAB) are proposed to have several beneficial effects, including the inactivation of carcinogens. We have studied the potential of Lactobacillus acidophilus (from a commercially available yogurt), Lactobacillus gasseri (P79), Lactobacillus confusus (DSM 20196), Streptococcus thermophilus (NCIM 50083), Bifidobacterium breve and Bifidobacterium longum (from human infant stool) t ...
- DOI:
- 10.1080/01635589609514492
- PubMed:
- 8910918
- https://doi.org/10.1080/01635589609514492
- Author:
- El-Mansouri, I.; Mercado, J.A.; Valpuesta, V.; Lopez-Aranda, J.M.; Pliego-Alfaro, F.; Quesada, M.A.
- Source:
- Plant cell reports 1996 v.15 no.8 pp. 642-646
- ISSN:
- 0721-7714
- Subject:
- developmental stages; plasmids; benzyladenine; shoots; dose response; Agrobacterium radiobacter; explants; gene expression; leaves; rooting; enzyme activity; culture media; genetic transformation; Fragaria vesca; gene transfer; kanamycin; transgenic plants; indole butyric acid; rapid methods; plant morphology; beta-glucuronidase
- Abstract:
- ... An efficient and reliable method for shoot regeneration from leaf disks of Fragaria vesca L. has been developed. This protocol has been successfully employed to obtain transformed plants using Agrobacterium tumefaciens as gene vector. Murashige and Skoog basal medium supplemented with benzyladenine 4 mg/l) and indole-3-butyric acid (0.25 mg/l) induced the maximum percentage of shoot regeneration ( ...
- DOI:
- 10.1007/BF00232469
- PubMed:
- 24178534
- https://doi.org/10.1007/BF00232469
9. Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens
- Author:
- Rashid, H.; Toriyama, K.; Hinata, K.
- Source:
- Plant cell reports 1996 v.15 no.11 pp. 799-803
- ISSN:
- 0721-7714
- Subject:
- hygromycin B; flowering; genes; benzyladenine; Moricandia arvensis; callus; shoots; dose response; Agrobacterium radiobacter; explants; naphthaleneacetic acid; leaves; enzyme activity; culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; internodes; cell suspension culture; genetic vectors; beta-glucuronidase
- Abstract:
- ... A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and beta-glucuronidase (GUS). Max ...
- DOI:
- 10.1007/BF00233143
- PubMed:
- 24178211
- https://doi.org/10.1007/BF00233143
- Author:
- Kikkert, J.R.; Hebert-Soule, D.; Wallace, P.G.; Striem, M.J.; Beisch, B.I.
- Source:
- Plant cell reports 1996 v.15 no.5 pp. 311-316
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; germination; dose response; kanamycin; beta-glucuronidase; transgenic plants; embryo (plant); Vitis; gene expression; phosphotransferases (phosphomutases); neomycin; enzyme activity; culture media; cell suspension culture
- Abstract:
- ... Transgenic plantlets of 'Chancellor' grapevine (Vitis L. complex interspecific hybrid) were produced via biolistic transformation. Embryogenic cell suspensions were bombarded with 1 micrometer tungsten particles coated with pBI426 which encodes a fusion peptide between beta-glucuronidase (GUS) and neomycin phosphotransferase II (NPIII). The fusion peptide is under the control of a double 35S Cauli ...
- DOI:
- 10.1007/BF00232362
- PubMed:
- 24178348
- https://doi.org/10.1007/BF00232362
- Author:
- Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
- Source:
- Plant cell reports 1997 v.16 no.5 pp. 291-294
- ISSN:
- 0721-7714
- Subject:
- Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
- Abstract:
- ... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
- DOI:
- 10.1007/BF01088283
- https://doi.org/10.1007/BF01088283
- Author:
- Gutierrez-E, M.A.; Luth, D.; Moore, G.A.
- Source:
- Plant cell reports 1997 v.16 no.11 pp. 745-753
- ISSN:
- 0721-7714
- Subject:
- developmental stages; benzyladenine; genes; shoots; dose response; Citrus tristeza virus; Agrobacterium radiobacter; explants; gene expression; coat proteins; Citrus aurantium; Citrus aurantiifolia; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; kanamycin; transgenic plants; internodes; beta-glucuronidase
- Abstract:
- ... Factors influencing transformation frequencies using the Agrobacterium-mediated protocol developed for Citrus seedling internodal stem segments in this laboratory were evaluated, with particular emphasis on decreasing the numbers of "escape" shoots produced. Although the use of a wild-type "shooty" Agrobacterium strain allowed relatively high frequencies of beta-glucuronidase positive (GUS+) shoot ...
- DOI:
- 10.1007/s002990050313
- https://doi.org/10.1007/s002990050313
- Author:
- Pena, L.; Cervera, M.; Juarez, J.; Navarro, A.; Pina, J.A.; Navarro, L.
- Source:
- Plant cell reports 1997 v.16 no.11 pp. 731-737
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; geneticin; kanamycin; dose response; transgenic plants; Citrus aurantiifolia; Agrobacterium radiobacter; explants; gene expression; histochemistry; micropropagation; enzyme activity; culture media; beta-glucuronidase; light
- Abstract:
- ... We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck x Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We now report on the optimization of the cocultivation, regeneration and selection ...
- DOI:
- 10.1007/s002990050311
- https://doi.org/10.1007/s002990050311
- Author:
- Chu, C.C.; Qu, N.; Bassuner, B.; Bauwe, H.
- Source:
- Plant cell reports 1997 v.16 no.10 pp. 715-718
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; promoter regions; dose response; Agrobacterium radiobacter; benzyladenine; reporter genes; naphthaleneacetic acid; Flaveria pringlei; photosynthesis; culture media; beta-glucuronidase
- Abstract:
- ... The dicot genus Flaveria (Asteraceae), besides species with C3 or C4 photosynthesis, contains taxa with a broad range of different states of transition between the two major photosynthetic types. We have developed a reproducible and efficient Agrobacterium-mediated method for the stable genetic transformation of the C3-C4 intermediate species F. pubescens. Fusion constructs of the reporter gene be ...
- DOI:
- 10.1007/s002990050308
- https://doi.org/10.1007/s002990050308
- Author:
- Veylder, L. de.; Montagu, M. van.; Inze, D.
- Source:
- Plant & cell physiology 1997 v.38 no.5 pp. 568-577
- ISSN:
- 0032-0781
- Subject:
- herbicide safeners; developmental stages; branched chain amino acids; acetolactate synthase; genes; benzene; roots; dose response; gene expression; Arabidopsis thaliana; apical meristems; amino acid sequences; enzyme inhibitors; herbicide resistance; enzyme activity; histochemistry; transgenic plants; chlorsulfuron; beta-glucuronidase
- Abstract:
- ... The potential use of a new chemical-inducible gene expression system in Arabidopsis thaliana has been examined. The system is based on the maize In2-2 promoter which is activated by benzenesulfonamide herbicide safeners. Plants transformed with the beta-glucuronidase (gus) reporter gene under the control of the In2-2 promoter were grown in the presence of different safeners and the induced GUS act ...
- DOI:
- 10.1093/oxfordjournals.pcp.a029206
- PubMed:
- 9210333
- https://doi.org/10.1093/oxfordjournals.pcp.a029206
- Author:
- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 419-428
- ISSN:
- 0032-0889
- Subject:
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- Abstract:
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
- Author:
- Mullins, K.V.; Llewellyn, D.J.; Hartney, V.J.; Strauss, S.; Dennis, E.S.
- Source:
- Plant cell reports 1997 v.16 no.11 pp. 787-791
- ISSN:
- 0721-7714
- Subject:
- benzyladenine; dose response; Agrobacterium radiobacter; explants; Eucalyptus marginata; gene expression; clones; naphthaleneacetic acid; Eucalyptus grandis; leaves; culture media; indole acetic acid; Eucalyptus microtheca; genetic transformation; gene transfer; kanamycin; Eucalyptus camaldulensis; transgenic plants; reporter genes; genetic variation; beta-glucuronidase
- Abstract:
- ... Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some clones of E. microtheca, E. ochrophloia, E. grandis and E. marginata, but at lower ...
- DOI:
- 10.1007/s002990050321
- https://doi.org/10.1007/s002990050321
- Author:
- Krens, F.A.; Keizer, L.C.P.; Capel, I.E.M.
- Source:
- Plant cell reports 1997 v.17 no.1 pp. 39-43
- ISSN:
- 0721-7714
- Subject:
- benzyladenine; genetic engineering; micropropagation; 2,4-D; Carum carvi; callus; shoots; dose response; hypocotyls; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; metabolism; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; cotyledons; beta-glucuronidase
- Abstract:
- ... Regeneration in caraway was obtained via two different routes. Hypocotyls showed delayed shoot formation after a callus phase and at relatively low frequencies. In contrast, high-frequency, direct regeneration occurred when cotyledonary node explants were used. Transient expression of beta-glucuronidase was monitored after inoculation of both explant types with Agrobacterium tumefaciens AGL0(pMOG4 ...
- DOI:
- 10.1007/s002990050348
- https://doi.org/10.1007/s002990050348
- Author:
- Ho, C.K.; Chang, S.H.; Tsay, J.Y.; Tsai, C.J.; Chiang, V.L.; Chen, Z.Z.
- Source:
- Plant cell reports 1998 v.17 no.9 pp. 675-680
- ISSN:
- 0721-7714
- Subject:
- developmental stages; benzyladenine; neomycin; 2,4-D; roots; callus; organogenesis; shoots; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; naphthaleneacetic acid; phosphotransferases (phosphomutases); enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; Eucalyptus camaldulensis; transgenic plants; indole butyric acid; plant morphology; beta-glucuronidase
- Abstract:
- ... An efficient system for Agrobacterium-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants was developed. Transformation was accomplished by cocultivation of hypocotyl segments with Agrobacterium tumefaciens containing a binary Ti-plasmid vector harboring chimeric neomycin phosphotransferase and beta-glucuronidase (GUS) genes. A modified Gamborg's B5 medium used ...
- DOI:
- 10.1007/s002990050464
- https://doi.org/10.1007/s002990050464
- Author:
- Seabra, R.C.; Pais, M.S.
- Source:
- Plant cell reports 1998 v.17 no.3 pp. 177-182
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; in vitro culture; kanamycin; hypocotyls; dose response; transgenic plants; Agrobacterium radiobacter; developmental stages; gene expression; histochemistry; genes; benzyladenine; micropropagation; Castanea sativa; enzyme activity; plant morphology; culture media; beta-glucuronidase
- Abstract:
- ... Stable incorporation of the nptII gene into Castanea sativa Mill. has been achieved by Agrobacterium- mediated transformation. The transformation assays were performed by infecting wounded hypocotyls with a strain of Agrobacterium tumefaciens, LBA 4404 harbouring the plasmid p35SGUSINT. Although two schemes of selection were tested, many escapes were obtained. The best strategy to avoid this probl ...
- DOI:
- 10.1007/s002990050374
- https://doi.org/10.1007/s002990050374
21. Regeneration of transgenic plants of Mexican lime from Agrobacterium rhizogenes-transformed tissues
- Author:
- Perez-Molphe-Balch, E.; Ochoa-Alejo, N.
- Source:
- Plant cell reports 1998 v.17 no.8 pp. 591-596
- ISSN:
- 0721-7714
- Subject:
- developmental stages; Rhizobium rhizogenes; genes; benzyladenine; micropropagation; roots; shoots; dose response; explants; gene expression; naphthaleneacetic acid; cefotaxime; Citrus aurantiifolia; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; kanamycin; transgenic plants; plant morphology; beta-glucuronidase
- Abstract:
- ... Transgenic Mexican lime [Citrus aurantifolia (Christm.) Swing] plants were regenerated from tissues transformed by Agrobacterium rhizogenes strain A4, containing the wild-type plasmid pRiA4 and the binary vector pESC4 with nos-npt II and cab-gus genes. Transgenic shoots were generated by two different approaches. The first approach used internodal stem segments cocultured with A. rhizogenes. These ...
- DOI:
- 10.1007/s002990050448
- https://doi.org/10.1007/s002990050448
- Author:
- Kokko, H.I.; Karenlampi, S.O.
- Source:
- Plant cell reports 1998 v.17 no.10 pp. 822-826
- ISSN:
- 0721-7714
- Subject:
- plasmids; benzyladenine; micropropagation; in vitro culture; dose response; stems; Agrobacterium radiobacter; gene expression; isopentenyladenine; naphthaleneacetic acid; kinetin; enzyme activity; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; indole butyric acid; Rubus arcticus; genetic vectors; beta-glucuronidase
- Abstract:
- ... Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA 101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which beta-glucuronidase (GUS) is expressed only in plants. Regenerants were produced on Murashige and Skoog mediu ...
- DOI:
- 10.1007/s002990050491
- https://doi.org/10.1007/s002990050491
- Author:
- Collen, A.M.C.; Jarl, C.I.
- Source:
- Plant cell reports 1999 v.19 no.1 pp. 13-19
- ISSN:
- 0721-7714
- Subject:
- developmental stages; benzyladenine; genes; micropropagation; 2,4-D; Agrobacterium; Galega orientalis; callus; shoots; dose response; meristems; explants; gene expression; naphthaleneacetic acid; enzyme activity; histochemistry; culture media; indole acetic acid; electroporation; genetic transformation; gene transfer; beta-glucuronidase
- Abstract:
- ... For the first time, regeneration and transformation have been achieved from the legume Galega orientalis Lam.(goat's rue). Two different regeneration protocols are described, one based on direct shoot induction from meristems and the other involving callus induction and shoot induction from callus with the plant growth regulator thidiazuron (TDZ). Different media and explants were evaluated. Three ...
- DOI:
- 10.1007/s002990050703
- https://doi.org/10.1007/s002990050703
- Author:
- Moriwaki, M.; Yamakawa, T.; Washino, T.; Kodama, T.; Igarashi, Y.
- Source:
- Plant cell reports 1999 v.19 no.1 pp. 96-100
- ISSN:
- 0721-7714
- Subject:
- heat shock proteins; genetic transformation; Arabidopsis thaliana; gene transfer; promoter regions; in vitro culture; dose response; transgenic plants; plant growth substances; stress response; Nicotiana plumbaginifolia; gene expression; genes; micropropagation; temperature; enzyme activity; culture media; beta-glucuronidase
- Abstract:
- ... The expression of the Arabidopsis heat shock protein (HSP) 18.2 promoter-beta-D-glucuronidase (GUS) chimera gene was investigated in transgenic Nicotiana plumbaginifolia plants during the recovery phase at normal temperatures (20-22 degrees C) after a heat shock (HS) treatment. GUS activity increased during the recovery phase after HS at 42 degrees C for 2 h, and maximal GUS activity was observed ...
- DOI:
- 10.1007/s002990050717
- https://doi.org/10.1007/s002990050717
- Author:
- Yang, J.; Lee, H.J.; Shin, D.H.; Oh, S.K.; Seon, J.H.; Paek, K.Y.; Han, K.H.
- Source:
- Plant cell reports 1999 v.18 no.12 pp. 978-984
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; in vitro culture; dose response; cell growth; transgenic plants; gene expression; genes; Cymbidium; benzyladenine; naphthaleneacetic acid; enzyme activity; culture media; beta-glucuronidase; shoots
- Abstract:
- ... A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH20 ...
- DOI:
- 10.1007/s002990050694
- https://doi.org/10.1007/s002990050694
- Author:
- Moriwaki, M.; Yamakawa, T.; Washino, T.; Kodama, T.; Igarashi, Y.
- Source:
- Plant cell reports 1999 v.19 no.1 pp. 92-95
- ISSN:
- 0721-7714
- Subject:
- genes; micropropagation; Nicotiana plumbaginifolia; promoter regions; in vitro culture; dose response; gene expression; leaves; Arabidopsis thaliana; temperature; enzyme activity; culture media; heat shock proteins; genetic transformation; gene transfer; transgenic plants; plant growth substances; stress response; flowers; beta-glucuronidase
- Abstract:
- ... The expression of the Arabidopsis heat-shock protein (HSP) 18.2 promoter beta-D-glucuronidase (GUS) chimera gene was studied in various organs of the transgenic Nicotiana plumbaginifolia during the recovery phase at normal temperatures (20-22 degrees C) following heat-shock (HS) treatment. The optimum HS temperature for GUS activity in the anthers, petals and capsules was 42 degrees C, but in imma ...
- DOI:
- 10.1007/s002990050716
- https://doi.org/10.1007/s002990050716
- Author:
- Miguel, C.M.; Oliveira, M.M.
- Source:
- Plant cell reports 1999 v.18 no.5 pp. 387-393
- ISSN:
- 0721-7714
- Subject:
- developmental stages; benzyladenine; micropropagation; neomycin; genetic markers; dose response; Agrobacterium radiobacter; explants; gene expression; leaves; phosphotransferases (phosphomutases); enzyme activity; culture media; Prunus dulcis; indole acetic acid; genetic transformation; gene transfer; transgenic plants; indole butyric acid; plant morphology; beta-glucuronidase
- Abstract:
- ... Almond (Prunus dulcis Mill.) leaves were transformed with the marker genes gusA (beta-glucuronidase) and nptII (neomycin phosphotransferase II) via Agrobacterium-mediated transformation. Bacterial strains and pre-culture of explants affected efficiency of gene transfer evaluated by transient expression assays. Following transformation, shoots were induced from primary explants on medium without ka ...
- DOI:
- 10.1007/s002990050591
- https://doi.org/10.1007/s002990050591
- Author:
- Krishnamurthy, K.V.; Suhasini, K.; Sagare, A.P.; Meixner, M.; De Kathen, A.; Pickardt, T.; Schieder, O.
- Source:
- Plant cell reports 2000 v.19 no.3 pp. 235-240
- ISSN:
- 0721-7714
- Subject:
- developmental stages; benzyladenine; glufosinate; micropropagation; neomycin; Cicer arietinum; cell biology; shoots; in vitro culture; dose response; Agrobacterium radiobacter; clones; gene expression; cultivars; phosphotransferases (phosphomutases); enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; embryo (plant); genetic variation; plant morphology; beta-glucuronidase
- Abstract:
- ... Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 ...
- DOI:
- 10.1007/s002990050005
- https://doi.org/10.1007/s002990050005
- Author:
- Park, S.U.; Facchini, P.J.
- Source:
- Plant cell reports 2000 v.19 no.10 pp. 1006-1012
- ISSN:
- 0721-7714
- Subject:
- isoquinoline alkaloids; developmental stages; genes; micropropagation; neomycin; dose response; Agrobacterium radiobacter; gene expression; naphthaleneacetic acid; phosphotransferases (phosphomutases); Eschscholzia californica; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; biosynthesis; transgenic plants; cotyledons; somatic embryogenesis; beta-glucuronidase
- Abstract:
- ... An efficient Agrobacterium-mediated protocol for the stable genetic transformation of Eschscholzia californica Cham. (California poppy) via somatic embryogenesis is reported. Excised cotyledons were co-cultivated with A. tumefaciens strain GV3101 carrying the pBI121 binary vector. Except for the co-cultivation medium, all formulations included 50 mg l-1 paromomycin as the selective agent and 200 m ...
- DOI:
- 10.1007/s002990000213
- https://doi.org/10.1007/s002990000213
- Author:
- Belarmino, M.M.; Mii, M.
- Source:
- Plant cell reports 2000 v.19 no.5 pp. 435-442
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; hybrids; in vitro culture; abscisic acid; dose response; plant anatomy; transgenic plants; Agrobacterium radiobacter; developmental stages; gene expression; histochemistry; benzyladenine; naphthaleneacetic acid; Phalaenopsis; enzyme activity; culture media; beta-glucuronidase
- DOI:
- 10.1007/s002990050752
- https://doi.org/10.1007/s002990050752
- Author:
- Zhang, F.L.; Takahata, Y.; Watanabe, M.; Xu, J.B.
- Source:
- Plant cell reports 2000 v.19 no.6 pp. 569-575
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; cultivars; Brassica rapa subsp. pekinensis; kanamycin; dose response; transgenic plants; Agrobacterium radiobacter; explants; cotyledons; gene expression; benzyladenine; reporter genes; naphthaleneacetic acid; genetic variation; enzyme activity; culture media; beta-glucuronidase
- Abstract:
- ... A procedure for producing transgenic Chinese cabbage plants by inoculating cotyledonary explants with Agrobacterium tumefaciens strain EHA101 carrying a binary vector pIG121Hm, which contains kanamycin-resistance and hygromycin-resistance genes and the GUS reporter gene, is described. Infection was most effective (highest infection frequency) when explants were infected with Agrobacterium for 15 m ...
- DOI:
- 10.1007/s002990050775
- https://doi.org/10.1007/s002990050775
- Author:
- Montoro, P.; Teinseree, N.; Rattana, W.; Kongsawadworakul, P.; Michaux-Ferriere, N.
- Source:
- Plant cell reports 2000 v.19 no.9 pp. 851-855
- ISSN:
- 0721-7714
- Subject:
- Hevea brasiliensis; genetic transformation; gene transfer; in vitro culture; dose response; transgenic plants; Agrobacterium radiobacter; gene expression; benzyladenine; micropropagation; abscisic acid; culture media; beta-glucuronidase; callus
- Abstract:
- ... The influence of CaCl(2) was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl(2). Five A. tumefaciens strains C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the strain EHA105pC2301 was selected to conduct ...
- DOI:
- 10.1007/s002990000208
- https://doi.org/10.1007/s002990000208
- Author:
- Hall, R.M.; Drew, R.A.; Higgins, C.M.; Dietzgen, R.G.
- Source:
- Australian journal of botany 2000 v.48 no.5 pp. 673-680
- ISSN:
- 0067-1924
- Subject:
- benzyladenine; genes; micropropagation; shoots; organogenesis; hybrids; dose response; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; coconut milk; enzyme activity; histochemistry; culture media; gene transfer; transgenic plants; cotyledons; beta-glucuronidase; Passiflora edulis; Queensland
- DOI:
- 10.1071/BT99067
- https://doi.org/10.1071/BT99067
- Author:
- Niu, X.; Li, X.; Veronese, P.; Bressan, R.A.; Weller, S.C.; Hasegawa, P.M.
- Source:
- Plant cell reports 2000 v.19 no.3 pp. 304-310
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; dose response; transgenic plants; Agrobacterium radiobacter; enzyme activity; developmental stages; explants; gene expression; histochemistry; genes; leaves; Mentha piperita; culture media; beta-glucuronidase; genetic markers
- Abstract:
- ... Substantial improvement in peppermint (Mentha x piperita L. Black Mitcham) genetic transformation has been achieved so that the frequency of transgenic plants regenerated (percent of leaf explants that produced transformed plants) was 20-fold greater than with the original protocol. Essential modifications were made to conditions for Agrobacterium tumefaciens co-cultivation that enhanced infection ...
- DOI:
- 10.1007/s002990050017
- https://doi.org/10.1007/s002990050017
- Author:
- Hosokawa, K.; Matsuki, R.; Oikawa, Y.; Yamamura, S.
- Source:
- Plant cell reports 2000 v.19 no.5 pp. 454-458
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; dose response; beta-glucuronidase; transgenic plants; enzyme activity; culture media; gene expression; phosphotransferases (phosphomutases); genes; naphthaleneacetic acid; Gentiana; histochemistry; cell suspension culture
- DOI:
- 10.1007/s002990050755
- https://doi.org/10.1007/s002990050755
- Author:
- Kamate, K.; Rodriguez-Llorente, I.D.; Scholte, M.; Durand, P.; Ratet, P.; Kondorosi, E.; Kondorosi, A.; Trinh, T.H.
- Source:
- Plant cell reports 2000 v.19 no.7 pp. 647-653
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; in vitro culture; dose response; transgenic plants; enzyme activity; developmental stages; indole butyric acid; flowers; gene expression; plant morphology; benzyladenine; histochemistry; reporter genes; Medicago truncatula; 2,4-D; culture media; beta-glucuronidase
- DOI:
- 10.1007/s002999900168
- https://doi.org/10.1007/s002999900168
- Author:
- T. R. Ganapathi; N. S. Higgs; P. J. Balint-Kurti; C. J. Arntzen; G. D. May; J. M. Van Eck
- Source:
- Plant cell reports 2001 v.20 no.2 pp. 157-162
- ISSN:
- 0721-7714
- Subject:
- developmental stages; genes; benzyladenine; 2,4-D; dose response; Agrobacterium radiobacter; gibberellic acid; gene expression; cultivars; beta-glucuronidase; histology; enzyme activity; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; Musa acuminata; genetic variation; plant morphology; cell suspension culture; India
- Abstract:
- ... A protocol was developed for establishing embryogenic suspension cultures from in vitro-grown, thin shoot-tip sections of the banana cultivar Rasthali. The best medium for callus induction was an MS-based medium supplemented with 2 mg/l 2,4-D and 0.2 mg/l zeatin. The callus was transferred to liquid medium to establish embryogenic cell suspensions. These cultures were subsequently used for Agrobac ...
- DOI:
- 10.1007/s002990000287
- https://doi.org/10.1007/s002990000287
- Author:
- Tsukazaki, H.; Kuginuki, Y.; Aida, R.; Suzuki, T.
- Source:
- Plant cell reports 2002 v.21 no.3 pp. 257-262
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; dose response; hypocotyls; haploidy; transgenic plants; Agrobacterium radiobacter; explants; gene expression; acetosyringone; genes; micropropagation; Brassica oleracea var. capitata; inheritance (genetics); culture media; beta-glucuronidase; shoots
- Abstract:
- ... Doubled haploid lines, which have high levels of genetic uniformity, are suitable for physiological or genetic studies. We established a transformation system using a doubled haploid line of cabbage. Hypocotyl explants that had been precultured for 3 days on Murashige and Skoog medium containing 50 micromolar acetosyringone were inoculated and cocultured with Agrobacterium tumefaciens strain LBA44 ...
- DOI:
- 10.1007/s00299-002-0506-4
- https://doi.org/10.1007/s00299-002-0506-4
- Author:
- Zhao, Q.H.; Fisher, R.; Auer, C.
- Source:
- Plant growth regulation 2002 v.37 no.3 pp. 223-231
- ISSN:
- 0167-6903
- Subject:
- adenosine; developmental stages; genes; benzyladenine; shoot meristems; roots; promoter regions; callus; organogenesis; dose response; explants; gene expression; isopentenyladenine; naphthaleneacetic acid; Arabidopsis thaliana; enzyme activity; culture media; tissue culture; zeatin; cotyledons; plant morphology; beta-glucuronidase
- Abstract:
- ... Shoot organogenesis in Arabidopsis thaliana was studied with regard to the timing of key developmental phases and expression of the SHOOTMERISTEMLESS (STM) gene. Shoot regeneration in the highly organogenic ecotype C24 was affected by explant type and age. The percentage of C24 cotyledon explants producing shoots decreased from 90% to 26% when donor seedlings were more than 6 d old, but 96% of roo ...
- Author:
- Hu, Z.; Yang, J.; Guo, G.Q.; Zheng, G.C.
- Source:
- Plant cell reports 2002 v.21 no.3 pp. 233-237
- ISSN:
- 0721-7714
- Subject:
- medicinal plants; micropropagation; neomycin; callus; genetic markers; dose response; Agrobacterium radiobacter; explants; leaves; Lycium; phosphotransferases (phosphomutases); culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; embryo (plant); somatic embryogenesis; reporter genes; beta-glucuronidase
- Abstract:
- ... We have developed a reliable and high-frequency system of transformation and regeneration via somatic embryogenesis (SE) of Lycium barbarum. Leaf segments were co-cultivated with Agrobacterium tumefaciens EHA101 (pIG121Hm) carrying the neomycin phosphotransferase II gene as a selectable marker and an intron-beta-glucuronidase (GUS) gene as a reporter marker. On the medium for callus-induction, whi ...
- DOI:
- 10.1007/s00299-002-0462-z
- https://doi.org/10.1007/s00299-002-0462-z
- Author:
- Cho, H.J.; Widholm, J.M.
- Source:
- Plant cell, tissue, and organ culture 2002 v.69 no.3 pp. 259-269
- ISSN:
- 0167-6857
- Subject:
- developmental stages; Rhizobium rhizogenes; genes; benzyladenine; 2,4-D; shoots; in vitro culture; dose response; gene expression; in vitro regeneration; kinetin; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; transgenic plants; Astragalus sinicus; plant morphology; genetic vectors; beta-glucuronidase
- Abstract:
- ... An improved protocol for shoot regeneration from hairy roots transformed by Agrobacterium rhizogenes of the legume species Astragalus sinicus (Chinese milk vetch) has been developed. The A. rhizogenes strain DC-AR2 harboring the binary vector pBI121 which carries the uidA gene encoding beta-glucuronidase activity and the kanamycin resistance gene nptII, was used to transform cut ends of plantlet h ...
- Author:
- Cerda, F.; Aquea, F.; Gebauer, M.; Medina, C.; Arce-Johnson, P.
- Source:
- Plant cell, tissue, and organ culture 2002 v.70 no.3 pp. 251-257
- ISSN:
- 0167-6857
- Subject:
- Pinus radiata; developmental stages; genes; benzyladenine; micropropagation; 2,4-D; in vitro culture; dose response; Agrobacterium radiobacter; zygote; in vitro regeneration; somatic embryos; forest trees; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; abscisic acid; plant morphology; beta-glucuronidase
- Abstract:
- ... Embryogenic cultures from immature zygotic embryos of Pinus radiata seeds were established on semisolid proliferation medium with 2,4-D and BAP. Growing embryogenic masses containing embryonal cells and suspensor cells were subcultured on this media every 2 weeks. After 10 weeks, embryogenic masses (1.5 cm diameter) were transferred to a maturation medium containing ABA. Fully developed somatic em ...
- Author:
- D. Das; M. Reddy; K. Upadhyaya; S. Sopory
- Source:
- Plant cell reports 2002 v.20 no.11 pp. 999-1005
- ISSN:
- 0721-7714
- Subject:
- grapes; developmental stages; light intensity; benzyladenine; micropropagation; cell differentiation; 2,4-D; callus; dose response; Agrobacterium radiobacter; gibberellic acid; explants; genotype; Vitis vinifera; leaves; cultivars; histology; temperature; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; embryo (plant); indole butyric acid; somatic embryogenesis; reporter genes; genetic variation; plant morphology; beta-glucuronidase; India
- Abstract:
- ... By manipulating hormone levels, light intensities and temperature, we have developed an efficient leaf-disc method for the regeneration of plants via embryogenesis and for transformation in four genotypes of Vitis viniferaL. In MS basal medium supplemented with 1 mg l–¹6-benzylaminopurine (BAP) and 0.1 mg l–¹2,4-dichlorophenoxyacetic acid, leaf discs cultured for 2 weeks under dark conditions prod ...
- DOI:
- 10.1007/s00299-002-0441-4
- https://doi.org/10.1007/s00299-002-0441-4
- Author:
- Imani, J.; Berting, A.; Nitsche, S.; Schaefer, S.; Gerlich, W.H.; Neumann, K.H.
- Source:
- Plant cell, tissue, and organ culture 2002 v.71 no.2 pp. 157-164
- ISSN:
- 0167-6857
- Subject:
- Hepatitis B virus; genes; 2,4-D; roots; callus; dose response; Agrobacterium radiobacter; Daucus carota; gene expression; naphthaleneacetic acid; carrots; in vitro regeneration; beta-glucuronidase; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; somatic embryogenesis; cultured cells; viral proteins; cell cycle; cell suspension culture
- Abstract:
- ... The percentage of cells successfully transformed by Agrobacteria is usually very low (not more than 10% and usually much less; Thomas et al., 1989). In this report we show that in carrot (Daucus carota L., ssp. sativus) cell suspensions transformation efficiency was strongly improved by using cell cycle synchronized cells. Fluorodesoxyuridine (FDU) was added for 24 h to inhibit thymidine synthesis ...
- Author:
- Men, S.; Ming, X.; Liu, R.; Wei, C.; Li, Y.
- Source:
- Plant cell, tissue, and organ culture 2003 v.75 no.1 pp. 63-71
- ISSN:
- 0167-6857
- Subject:
- hygromycin B; benzyladenine; micropropagation; genetic markers; marker-assisted selection; Dendrobium; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; ornamental plants; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; transgenic plants; rapid methods; corms; reporter genes; genetic vectors; beta-glucuronidase; acetosyringone
- Abstract:
- ... A protocol was developed to obtain stable transgenic orchids (Dendrobium nobile) via Agrobacterium-mediated transformation of protocorm-like bodies (PLBs). Agrobacterium tumefaciens strains AGL1 and EHA105 were used, with each containing a binary vector pCAMBIA1301 with the hpt gene as a selectable marker for hygromycin resistance and an intron-containing β-glucuronidase gene (gus-int) as a report ...
- Author:
- Zaldivar-Cruz, J.M.; Ballina-Gomez, H.; Guerrero-Rodriguez, C.; Aviles-Berzunza, E.; Godoy-Hernandez, G.C.
- Source:
- Plant cell, tissue, and organ culture 2003 v.73 no.3 pp. 281-284
- ISSN:
- 0167-6857
- Subject:
- food coloring; benzyladenine; dye plants; in vitro culture; dose response; hypocotyls; Agrobacterium radiobacter; gene expression; cultivars; genetic improvement; enzyme activity; histochemistry; culture media; genetic transformation; gene transfer; Bixa orellana; transgenic plants; reporter genes; genetic variation; beta-glucuronidase
- Abstract:
- ... Hypocotyls from annatto seedlings, were inoculated with Agrobacterium tumefaciens harboring a binary vector, pBI.121 or pCAMBIA2301, containing the β-glucuronidase (gus) gene. Histochemical GUS assay of infected hypocotyls from two annatto varieties showed transient gus gene expression between 3 and 12 days after inoculation. ...
47. High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus)
- Author:
- Khan, M.R.; Rashid, H.; Ansar, M.; Chaudry, Z.
- Source:
- Plant cell, tissue, and organ culture 2003 v.75 no.3 pp. 223-231
- ISSN:
- 0167-6857
- Subject:
- oil crops; benzyladenine; genes; Brassica napus; 2,4-D; shoots; dose response; hypocotyls; Agrobacterium radiobacter; gibberellic acid; explants; varieties; naphthaleneacetic acid; in vitro regeneration; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; silver nitrate; beta-glucuronidase
- Abstract:
- ... Five different varieties of Brassica napus (Cyclone, Dunkled, Oscar, Rainbow and KS75) were tested for their regeneration response. Cyclone showed a very high frequency of regeneration (92%). The use of silver nitrate was a pre-requisite for efficient shoot regeneration. Hypocotyls were selected as the starting material for transformation experiments on the basis of high transient GUS expression. ...
- Author:
- Cao, X.; Fordham, I.; Douglass, L.; Hammerschlag, F.
- Source:
- Plant cell, tissue, and organ culture 2003 v.75 no.3 pp. 255-259
- ISSN:
- 0167-6857
- Subject:
- in vitro regeneration; genetic transformation; gene transfer; cultivars; dose response; sucrose; Agrobacterium radiobacter; transgenic plants; explants; gene expression; leaves; genes; genetic variation; blueberries; culture media; beta-glucuronidase; Vaccinium corymbosum; shoots
- Abstract:
- ... In an effort to increase in vitro blueberry (Vaccinium corymbosum L.) shoot production without negatively impacting subsequent genetic engineering experiments, studies were conducted to examine the effects of sucrose concentration in the propagation medium on shoot proliferation and on the transfer of an intron-containing β-glucuronidase (GUS) gene into leaf explants from the propagated shoots. Nu ...
- Author:
- Tang, W.; Luo, H.; Newton, R.J.
- Source:
- Plant cell, tissue, and organ culture 2004 v.79 no.1 pp. 71-81
- ISSN:
- 0167-6857
- Subject:
- benzyladenine; micropropagation; Pinus taeda; 2,4-D; bacterial contamination; dose response; Agrobacterium radiobacter; explants; zygote; forest trees; culture media; genetic transformation; gene transfer; transgenic plants; embryo (plant); indole butyric acid; reporter genes; beta-glucuronidase; carbenicillin
- Abstract:
- ... Three antibiotics were evaluated for their effects on the elimination of Agrobacterium tumefaciens during the genetic transformation of loblolly pine (Pinus taeda L.) using mature zygotic embryos as targets. Agrobacterium tumefaciens strains, EHA105, GV3101, and LBA 4404, all harbouring the plasmid pCAMBIA1301, which carries the selectable marker gene, hygromycin phosphotransferase (hpt) controlle ...
- Author:
- Shiba, T.; Mii, M.
- Source:
- Journal of horticultural science & biotechnology 2005 v.80 no.4 pp. 393-398
- ISSN:
- 1462-0316
- Subject:
- hygromycin B; micropropagation; callus; dose response; Agrobacterium radiobacter; explants; gene expression; in vitro regeneration; leaves; ornamental plants; beta-glucuronidase; cut flowers; enzyme activity; Dianthus; culture media; genetic transformation; gene transfer; transgenic plants; cell suspension culture; acetosyringone