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1. Agrobacterium mediated transformation of chickpea (Cicer arietinum L.) embryo axes

Author:
Krishnamurthy, K.V.; Suhasini, K.; Sagare, A.P.; Meixner, M.; De Kathen, A.; Pickardt, T.; Schieder, O.
Source:
Plant cell reports 2000 v.19 no.3 pp. 235-240
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; glufosinate; micropropagation; neomycin; Cicer arietinum; cell biology; shoots; in vitro culture; dose response; Agrobacterium radiobacter; clones; gene expression; cultivars; phosphotransferases (phosphomutases); enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; embryo (plant); genetic variation; plant morphology; beta-glucuronidase
Abstract:
... Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 ...
DOI:
10.1007/s002990050005

2. Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants

Author:
Vergauwe, A.; Cammaert, R.; Vandenberghe, D.; Genetello, C.; Inze, D.; Montagu, M. van.; Eeckhout, E. van den.
Source:
Plant cell reports 1996 v.15 no.12 pp. 929-933
ISSN:
0721-7714
Subject:
benzyladenine; micropropagation; neomycin; 2,4-D; callus; shoots; dose response; Agrobacterium radiobacter; explants; naphthaleneacetic acid; leaves; polymerase chain reaction; Artemisia annua; rooting; phosphotransferases (phosphomutases); enzyme activity; culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; nucleotide sequences; beta-glucuronidase
Abstract:
... A transformation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphthaleneacetic acid and 0.5 mg/L N6-benzyladenine after transformation with the C58C1 Rif(R) (pGV2260)(pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Ka ...
DOI:
10.1007/BF00231590
PubMed:
24178277

3. Agrobacterium tumefaciens-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants

Author:
Ho, C.K.; Chang, S.H.; Tsay, J.Y.; Tsai, C.J.; Chiang, V.L.; Chen, Z.Z.
Source:
Plant cell reports 1998 v.17 no.9 pp. 675-680
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; neomycin; 2,4-D; roots; callus; organogenesis; shoots; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; naphthaleneacetic acid; phosphotransferases (phosphomutases); enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; Eucalyptus camaldulensis; transgenic plants; indole butyric acid; plant morphology; beta-glucuronidase
Abstract:
... An efficient system for Agrobacterium-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants was developed. Transformation was accomplished by cocultivation of hypocotyl segments with Agrobacterium tumefaciens containing a binary Ti-plasmid vector harboring chimeric neomycin phosphotransferase and beta-glucuronidase (GUS) genes. A modified Gamborg's B5 medium used ...
DOI:
10.1007/s002990050464

4. Agrobacterium tumefaciens-mediated transformation of Solanum gilo Raddi as influenced by explant type

Author:
Blay, E.; Oakes, J.V.
Source:
Plant cell reports 1996 v.15 no.8 pp. 582-585
ISSN:
0721-7714
Subject:
genetic transformation; dose response; kanamycin; hypocotyls; Solanum aethiopicum; beta-glucuronidase; transgenic plants; Agrobacterium radiobacter; explants; cotyledons; gene expression; histochemistry; culture media; roots; genetic markers
Abstract:
... An efficient system for Agrobacterium tumefaciens-mediated transformation of Solanum gilo was established. The marker genes for kanamycin resistance and beta-glucuronidase expression were introduced. A comparison between cotyledon and hypocotyl explants showed that while regeneration was better from hypocotyl explants, cotyledon explants gave better transformation efficiency (46% vs. 32%). Four le ...
DOI:
10.1007/BF00232457
PubMed:
24178522

5. Agrobacterium tumefaciens-mediated transformation of highly regenerable cell suspension cultures in Dianthus acicularis

Author:
Shiba, T.; Mii, M.
Source:
Journal of horticultural science & biotechnology 2005 v.80 no.4 pp. 393-398
ISSN:
1462-0316
Subject:
hygromycin B; micropropagation; callus; dose response; Agrobacterium radiobacter; explants; gene expression; in vitro regeneration; leaves; ornamental plants; beta-glucuronidase; cut flowers; enzyme activity; Dianthus; culture media; genetic transformation; gene transfer; transgenic plants; cell suspension culture; acetosyringone

6. Agrobacterium-mediated genetic transformation of California poppy, Eschscholzia californica Cham., via somatic embryogenesis

Author:
Park, S.U.; Facchini, P.J.
Source:
Plant cell reports 2000 v.19 no.10 pp. 1006-1012
ISSN:
0721-7714
Subject:
isoquinoline alkaloids; developmental stages; genes; micropropagation; neomycin; dose response; Agrobacterium radiobacter; gene expression; naphthaleneacetic acid; phosphotransferases (phosphomutases); Eschscholzia californica; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; biosynthesis; transgenic plants; cotyledons; somatic embryogenesis; beta-glucuronidase
Abstract:
... An efficient Agrobacterium-mediated protocol for the stable genetic transformation of Eschscholzia californica Cham. (California poppy) via somatic embryogenesis is reported. Excised cotyledons were co-cultivated with A. tumefaciens strain GV3101 carrying the pBI121 binary vector. Except for the co-cultivation medium, all formulations included 50 mg l-1 paromomycin as the selective agent and 200 m ...
DOI:
10.1007/s002990000213

7. Agrobacterium-mediated genetic transformation of a Dendrobium orchid

Author:
Men, S.; Ming, X.; Liu, R.; Wei, C.; Li, Y.
Source:
Plant cell, tissue, and organ culture 2003 v.75 no.1 pp. 63-71
ISSN:
0167-6857
Subject:
hygromycin B; benzyladenine; micropropagation; genetic markers; marker-assisted selection; Dendrobium; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; ornamental plants; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; transgenic plants; rapid methods; corms; reporter genes; genetic vectors; beta-glucuronidase; acetosyringone
Abstract:
... A protocol was developed to obtain stable transgenic orchids (Dendrobium nobile) via Agrobacterium-mediated transformation of protocorm-like bodies (PLBs). Agrobacterium tumefaciens strains AGL1 and EHA105 were used, with each containing a binary vector pCAMBIA1301 with the hpt gene as a selectable marker for hygromycin resistance and an intron-containing β-glucuronidase gene (gus-int) as a report ...

8. Agrobacterium-mediated genetic transformation of a phalaenopsis orchid

Author:
Belarmino, M.M.; Mii, M.
Source:
Plant cell reports 2000 v.19 no.5 pp. 435-442
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; hybrids; in vitro culture; abscisic acid; dose response; plant anatomy; transgenic plants; Agrobacterium radiobacter; developmental stages; gene expression; histochemistry; benzyladenine; naphthaleneacetic acid; Phalaenopsis; enzyme activity; culture media; beta-glucuronidase
DOI:
10.1007/s002990050752

9. Agrobacterium-mediated transformation of a doubled haploid line of cabbage

Author:
Tsukazaki, H.; Kuginuki, Y.; Aida, R.; Suzuki, T.
Source:
Plant cell reports 2002 v.21 no.3 pp. 257-262
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; dose response; hypocotyls; haploidy; transgenic plants; Agrobacterium radiobacter; explants; gene expression; acetosyringone; genes; micropropagation; Brassica oleracea var. capitata; inheritance (genetics); culture media; beta-glucuronidase; shoots
Abstract:
... Doubled haploid lines, which have high levels of genetic uniformity, are suitable for physiological or genetic studies. We established a transformation system using a doubled haploid line of cabbage. Hypocotyl explants that had been precultured for 3 days on Murashige and Skoog medium containing 50 micromolar acetosyringone were inoculated and cocultured with Agrobacterium tumefaciens strain LBA44 ...
DOI:
10.1007/s00299-002-0506-4

10. Agrobacterium-mediated transformation of apple (Malus X domestica Borkh.): an assessment of factors affecting regeneration of transgenic plants

Author:
Bondt, A. de; Eggermont, K.; penninckx, I.; Goderis, I.; Browkaert, W.F.
Source:
Plant cell reports 1996 v.15 no.7 pp. 549-554
ISSN:
0721-7714
Subject:
gels; glufosinate; genetic markers; shoots; dose response; Agrobacterium radiobacter; explants; gene expression; Malus domestica; leaves; cultivars; phosphinothricin acetyltransferase; enzyme activity; culture media; genetic transformation; kanamycin; transgenic plants; zeatin; genetic variation; beta-glucuronidase
Abstract:
... We have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. Jonagold) explants with Agrobacterium tumefaciens (De Bondt et al. 1994, Plant Cell Reports 13: 587-593). Now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultiva ...
DOI:
10.1007/BF00232992
PubMed:
24178471

11. Agrobacterium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Author:
Zhang, F.L.; Takahata, Y.; Watanabe, M.; Xu, J.B.
Source:
Plant cell reports 2000 v.19 no.6 pp. 569-575
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; cultivars; Brassica rapa subsp. pekinensis; kanamycin; dose response; transgenic plants; Agrobacterium radiobacter; explants; cotyledons; gene expression; benzyladenine; reporter genes; naphthaleneacetic acid; genetic variation; enzyme activity; culture media; beta-glucuronidase
Abstract:
... A procedure for producing transgenic Chinese cabbage plants by inoculating cotyledonary explants with Agrobacterium tumefaciens strain EHA101 carrying a binary vector pIG121Hm, which contains kanamycin-resistance and hygromycin-resistance genes and the GUS reporter gene, is described. Infection was most effective (highest infection frequency) when explants were infected with Agrobacterium for 15 m ...
DOI:
10.1007/s002990050775

12. Agrobacterium-mediated transformed transgenic triploid bermudagrass (Cynodon dactylon x C. transvaalensis) plants are highly resistant to the glufosinate herbicide Liberty

Author:
Hu, F.; Zhang, L.; Wang, X.; Ding, J.; Wu, D.
Source:
Plant cell, tissue, and organ culture 2005 v.83 no.1 pp. 13-19
ISSN:
0167-6857
Subject:
Cynodon dactylon; glufosinate; genes; benzyladenine; micropropagation; 2,4-D; Cynodon transvaalensis; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; casein hydrolysates; naphthaleneacetic acid; turf grasses; herbicide resistance; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; abscisic acid; beta-glucuronidase; acetosyringone
Abstract:
... Glufosinate resistance gene isolated from Streptomyces hygromicinroscopicus (bar) that confers the resistance of herbicide Liberty, a broad-spectrum grass and broadleaf contact herbicide widely used for weed control, was introduced into triploid bermudagrass by Agrobacterium-mediated transformation. Embryogenic calluses derived from stolonous nodal segment were co-cultured with the disarmed strain ...

13. Agrobacterium-mediated transient transformation of annatto (Bixa orellana) hypocotyls with the gus reporter gene

Author:
Zaldivar-Cruz, J.M.; Ballina-Gomez, H.; Guerrero-Rodriguez, C.; Aviles-Berzunza, E.; Godoy-Hernandez, G.C.
Source:
Plant cell, tissue, and organ culture 2003 v.73 no.3 pp. 281-284
ISSN:
0167-6857
Subject:
food coloring; benzyladenine; dye plants; in vitro culture; dose response; hypocotyls; Agrobacterium radiobacter; gene expression; cultivars; genetic improvement; enzyme activity; histochemistry; culture media; genetic transformation; gene transfer; Bixa orellana; transgenic plants; reporter genes; genetic variation; beta-glucuronidase
Abstract:
... Hypocotyls from annatto seedlings, were inoculated with Agrobacterium tumefaciens harboring a binary vector, pBI.121 or pCAMBIA2301, containing the β-glucuronidase (gus) gene. Histochemical GUS assay of infected hypocotyls from two annatto varieties showed transient gus gene expression between 3 and 12 days after inoculation. ...

14. Agrobacterium -mediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (AAB)

Author:
T. R. Ganapathi; N. S. Higgs; P. J. Balint-Kurti; C. J. Arntzen; G. D. May; J. M. Van Eck
Source:
Plant cell reports 2001 v.20 no.2 pp. 157-162
ISSN:
0721-7714
Subject:
developmental stages; genes; benzyladenine; 2,4-D; dose response; Agrobacterium radiobacter; gibberellic acid; gene expression; cultivars; beta-glucuronidase; histology; enzyme activity; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; Musa acuminata; genetic variation; plant morphology; cell suspension culture; India
Abstract:
... A protocol was developed for establishing embryogenic suspension cultures from in vitro-grown, thin shoot-tip sections of the banana cultivar Rasthali. The best medium for callus induction was an MS-based medium supplemented with 2 mg/l 2,4-D and 0.2 mg/l zeatin. The callus was transferred to liquid medium to establish embryogenic cell suspensions. These cultures were subsequently used for Agrobac ...
DOI:
10.1007/s002990000287

15. Andalusol, a Diterpenoid with anti-inflammatory Activity from Siderits foetens Clemen

Author:
A. Navarro; B. de las Heras
Source:
Zeitschrift für Naturforschung 2014 v.52 no.11-12 pp. 844-849
ISSN:
1865-7125
Subject:
Sideritis; acetates; acetone; anti-inflammatory activity; beta-glucuronidase; calcium ionophores; carrageenan; diterpenoids; dose response; histamine; lactate dehydrogenase; mast cells; myeloperoxidase; neutrophils; rats; topical application; toxicity
Abstract:
... The anti-inflammatory activity of andalusol (ent-13(16),14-labdadiene-6α,8α,18-triol), a diterpenoid obtained from the acetone extract of Sideritis foetens Clemen, has been investigated. This compound was able to inhibit acute inflammatory processes induced by carrageenan or 12-O -tetradecanoylphorbol acetate (TPA) after oral or topical administration. Quantitation of the neutrophil specific marke ...
DOI:
10.1515/znc-1997-11-1219

16. Application of metabonomic strategy to discover an unreported active ingredient in LiuWeiDiHuang pills suppressing beta-glucuronidase

Author:
Baogang Xie; Zhirong Zhang; Tao Gong; Ningning Zhang; Huiyun Wang; Huiqing Zou
Source:
Analytical and bioanalytical chemistry 2015 v.407 no.2 pp. 609-614
ISSN:
1618-2642
Subject:
Oriental traditional medicine; active ingredients; beta-glucuronidase; bioactive properties; daidzein; discriminant analysis; dose response; drugs; genistein; high performance liquid chromatography; least squares; liver; mass spectrometry; metabolomics; nuclear magnetic resonance spectroscopy; rats; screening; urine
Abstract:
... Identification of the bioactive ingredient from traditional Chinese medicine (TCM) remains a challenging task by traditional approach that focuses on chemical isolation coupled with biological activity screening. Here, we present a metabonomics-based approach for bioactive ingredient discovery in LiuWeiDiHuang pills (LWPs). First, a non-targeted high-performance liquid chromatography ultraviolet ( ...
DOI:
10.1007/s00216-014-8253-2

17. Aryl hydrocarbon receptor (AhR)-mediated reporter gene expression systems in transgenic tobacco plants

Author:
Kodama, Susumu; Okada, Kumiko; Inui, Hideyuki; Ohkawa, Hideo
Source:
Planta 2007 v.227 no.1 pp. 37-45
ISSN:
0032-0935
Subject:
beta-glucuronidase; bioassays; dioxins; dose response; gene expression; herpes simplex; indigo; leaves; ligands; mice; reporter genes; soaking; tobacco; transgenic plants; viruses
Abstract:
... In mammals, the aryl hydrocarbon receptor (AhR) mediates expression of certain genes, including CYP1A1, in response to exposure to dioxins and related compounds. We have constructed a mouse AhR-mediated gene expression systems for a β-glucuronidase (GUS) reporter gene consisting of an AhR, an AhR nuclear translocator (Arnt), and a xenobiotic response element (XRE)-driven promoter in transgenic tob ...
DOI:
10.1007/s00425-007-0592-1
PubMed:
17879099

18. Calluses initiated from thin mature embryo fragments are suitable targets for wheat transformation as assessed by long-term GUS expression studies

Author:
Delporte, F.; Li, S.; Jacquemin, J.M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 139-149
ISSN:
0167-6857
Subject:
genetic transformation; dose response; biolistics; transgenic plants; embryo (plant); Agrobacterium radiobacter; explants; wheat; gene expression; Triticum aestivum; 2,4-D; micropropagation; enzyme activity; in vitro regeneration; culture media; beta-glucuronidase; callus
Abstract:
... Microprojectile- or Agrobacterium-mediated DNA delivery into calluses initiated from immature embryos has proven to be effective in transforming wheat. Yet, obtaining a large number of high quality immature embryos throughout the year is a laborious and delicate process. To circumvent these limitations, we propose an alternative technique applying the particle bombardment technology to calluses de ...

19. Comparison of different methods for plant regeneration and transformation of the legume Galega orientalis Lam. (goat's rue)

Author:
Collen, A.M.C.; Jarl, C.I.
Source:
Plant cell reports 1999 v.19 no.1 pp. 13-19
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; genes; micropropagation; 2,4-D; Agrobacterium; Galega orientalis; callus; shoots; dose response; meristems; explants; gene expression; naphthaleneacetic acid; enzyme activity; histochemistry; culture media; indole acetic acid; electroporation; genetic transformation; gene transfer; beta-glucuronidase
Abstract:
... For the first time, regeneration and transformation have been achieved from the legume Galega orientalis Lam.(goat's rue). Two different regeneration protocols are described, one based on direct shoot induction from meristems and the other involving callus induction and shoot induction from callus with the plant growth regulator thidiazuron (TDZ). Different media and explants were evaluated. Three ...
DOI:
10.1007/s002990050703

20. DNA methylation and Dc8-GUS transgene expression in carrot (Daucus carota L.)

Author:
Zhou, Y.; Magill, J.M.; Magill, C.W.; Newton, R.J.
Source:
Plant cell reports 1996 v.15 no.11 pp. 815-818
ISSN:
0721-7714
Subject:
Daucus carota; DNA methylation; transgenic plants; gene expression; genes; gene transfer; chimerism; beta-glucuronidase; enzyme activity; cytidine; abscisic acid; dose response; quantitative analysis
Abstract:
... DNA methylation has been associated with gene activity in differentiating and developing plant tissues. The objective of this study was to determine the involvement of methylation in the expression of a gene transferred into carrot (Daucus carota L.) tissues by particle bombardment. Expression of the Dc8-GUS gene construct in response to treatments with 5-azacytidine (5-azaC) and to in vitro methy ...
DOI:
10.1007/BF00233146
PubMed:
24178214

21. Delayed recovery of beta-glucuronidase activity driven by an Arabidopsis heat shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia

Author:
Moriwaki, M.; Yamakawa, T.; Washino, T.; Kodama, T.; Igarashi, Y.
Source:
Plant cell reports 1999 v.19 no.1 pp. 96-100
ISSN:
0721-7714
Subject:
heat shock proteins; genetic transformation; Arabidopsis thaliana; gene transfer; promoter regions; in vitro culture; dose response; transgenic plants; plant growth substances; stress response; Nicotiana plumbaginifolia; gene expression; genes; micropropagation; temperature; enzyme activity; culture media; beta-glucuronidase
Abstract:
... The expression of the Arabidopsis heat shock protein (HSP) 18.2 promoter-beta-D-glucuronidase (GUS) chimera gene was investigated in transgenic Nicotiana plumbaginifolia plants during the recovery phase at normal temperatures (20-22 degrees C) after a heat shock (HS) treatment. GUS activity increased during the recovery phase after HS at 42 degrees C for 2 h, and maximal GUS activity was observed ...
DOI:
10.1007/s002990050717

22. Developmental phases and STM expression during Arabidopsis shoot organogenesis

Author:
Zhao, Q.H.; Fisher, R.; Auer, C.
Source:
Plant growth regulation 2002 v.37 no.3 pp. 223-231
ISSN:
0167-6903
Subject:
adenosine; developmental stages; genes; benzyladenine; shoot meristems; roots; promoter regions; callus; organogenesis; dose response; explants; gene expression; isopentenyladenine; naphthaleneacetic acid; Arabidopsis thaliana; enzyme activity; culture media; tissue culture; zeatin; cotyledons; plant morphology; beta-glucuronidase
Abstract:
... Shoot organogenesis in Arabidopsis thaliana was studied with regard to the timing of key developmental phases and expression of the SHOOTMERISTEMLESS (STM) gene. Shoot regeneration in the highly organogenic ecotype C24 was affected by explant type and age. The percentage of C24 cotyledon explants producing shoots decreased from 90% to 26% when donor seedlings were more than 6 d old, but 96% of roo ...

23. Differential immune responses of the green neon shrimp (Neocaridina denticulate) to dipropyl phthalate

Author:
Hung-Hung Sung; Yi-Hsiu Lin; Chun-Yi Hsiao
Source:
Fish & shellfish immunology 2011 v.31 no.3 pp. 511-515
ISSN:
1050-4648
Subject:
Aeromonas veronii; acid phosphatase; bacterial infections; beta-glucuronidase; dose response; hemocyanin; immune response; messenger RNA; monophenol monooxygenase; mortality; phthalates; shrimp; sublethal effects; superoxide dismutase
Abstract:
... This study set out to understand the sublethal effect of xenobiotic phthalate esters (PAEs) on the relationship between the susceptibility of shrimp to bacterial infection and the immune response of the shrimp. Neocaridina denticulate were exposed to different concentrations of the PAE dipropyl phthalate (DPrP), and mortality and six immune parameters were measured on days 1, 3, 5, and 10 after ex ...
DOI:
10.1016/j.fsi.2011.06.021

24. Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli

Author:
Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 291-294
ISSN:
0721-7714
Subject:
Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
Abstract:
... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
DOI:
10.1007/BF01088283

25. Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli

Author:
Montoro, P.; Teinseree, N.; Rattana, W.; Kongsawadworakul, P.; Michaux-Ferriere, N.
Source:
Plant cell reports 2000 v.19 no.9 pp. 851-855
ISSN:
0721-7714
Subject:
Hevea brasiliensis; genetic transformation; gene transfer; in vitro culture; dose response; transgenic plants; Agrobacterium radiobacter; gene expression; benzyladenine; micropropagation; abscisic acid; culture media; beta-glucuronidase; callus
Abstract:
... The influence of CaCl(2) was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl(2). Five A. tumefaciens strains C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the strain EHA105pC2301 was selected to conduct ...
DOI:
10.1007/s002990000208

26. Effects of antibiotics on the elimination of Agrobacterium tumefaciens from loblolly pine (Pinus taeda) zygotic embryo explants and on transgenic plant regeneration

Author:
Tang, W.; Luo, H.; Newton, R.J.
Source:
Plant cell, tissue, and organ culture 2004 v.79 no.1 pp. 71-81
ISSN:
0167-6857
Subject:
benzyladenine; micropropagation; Pinus taeda; 2,4-D; bacterial contamination; dose response; Agrobacterium radiobacter; explants; zygote; forest trees; culture media; genetic transformation; gene transfer; transgenic plants; embryo (plant); indole butyric acid; reporter genes; beta-glucuronidase; carbenicillin
Abstract:
... Three antibiotics were evaluated for their effects on the elimination of Agrobacterium tumefaciens during the genetic transformation of loblolly pine (Pinus taeda L.) using mature zygotic embryos as targets. Agrobacterium tumefaciens strains, EHA105, GV3101, and LBA 4404, all harbouring the plasmid pCAMBIA1301, which carries the selectable marker gene, hygromycin phosphotransferase (hpt) controlle ...

27. Effects of antioxidants on the plant regeneration and GUS expressive frequency of peanut (Arachis hypogaea) explants by Agrobacterium tumefaciens

Author:
Zheng, Q..; Ju, B.; Liang, L.; Xiao, X.
Source:
Plant cell, tissue, and organ culture 2005 v.81 no.1 pp. 83-90
ISSN:
0167-6857
Subject:
peanuts; glutathione; dose response; malondialdehyde; hydrogen peroxide; Agrobacterium radiobacter; explants; Arachis hypogaea; in vitro regeneration; superoxide dismutase; selenites; enzyme activity; culture media; ascorbic acid; genetic transformation; gene transfer; catalase; transgenic plants; beta-glucuronidase

28. Effects of carrot and tomato juice consumption on faecal markers relevant to colon carcinogenesis in humans

Author:
Schnabele, K.; Briviba, K.; Bub, A.; Roser, S.; Pool-Zobel, B.L.; Rechkemmer, G.
Source:
British journal of nutrition 2008 v.99 no.3 pp. 606-613
ISSN:
0007-1145
Subject:
disease prevention; alpha-carotene; short chain fatty acids; carcinogenesis; dose response; biomarkers; lycopene; feces; beta-carotene; tomato juice; carrot juice; beta-glucosidase; humans; cytotoxicity; adenocarcinoma; colorectal neoplasms; cell proliferation; food intake; dosage; cultured cells; pH; bile acids; beta-glucuronidase
DOI:
10.1017/S0007114507819143
PubMed:
18254985

29. Efficient organogenesis of an Australian passionfruit hybrid (Passiflora edulis x Passiflora edulis var. flavicarpa) suitable for gene delivery

Author:
Hall, R.M.; Drew, R.A.; Higgins, C.M.; Dietzgen, R.G.
Source:
Australian journal of botany 2000 v.48 no.5 pp. 673-680
ISSN:
0067-1924
Subject:
benzyladenine; genes; micropropagation; shoots; organogenesis; hybrids; dose response; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; coconut milk; enzyme activity; histochemistry; culture media; gene transfer; transgenic plants; cotyledons; beta-glucuronidase; Passiflora edulis; Queensland
DOI:
10.1071/BT99067

30. Factors affecting Agrobacterium tumefaciens-mediated transformation of peppermint

Author:
Niu, X.; Li, X.; Veronese, P.; Bressan, R.A.; Weller, S.C.; Hasegawa, P.M.
Source:
Plant cell reports 2000 v.19 no.3 pp. 304-310
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; dose response; transgenic plants; Agrobacterium radiobacter; enzyme activity; developmental stages; explants; gene expression; histochemistry; genes; leaves; Mentha piperita; culture media; beta-glucuronidase; genetic markers
Abstract:
... Substantial improvement in peppermint (Mentha x piperita L. Black Mitcham) genetic transformation has been achieved so that the frequency of transgenic plants regenerated (percent of leaf explants that produced transformed plants) was 20-fold greater than with the original protocol. Essential modifications were made to conditions for Agrobacterium tumefaciens co-cultivation that enhanced infection ...
DOI:
10.1007/s002990050017

31. Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus

Author:
Gutierrez-E, M.A.; Luth, D.; Moore, G.A.
Source:
Plant cell reports 1997 v.16 no.11 pp. 745-753
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; genes; shoots; dose response; Citrus tristeza virus; Agrobacterium radiobacter; explants; gene expression; coat proteins; Citrus aurantium; Citrus aurantiifolia; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; kanamycin; transgenic plants; internodes; beta-glucuronidase
Abstract:
... Factors influencing transformation frequencies using the Agrobacterium-mediated protocol developed for Citrus seedling internodal stem segments in this laboratory were evaluated, with particular emphasis on decreasing the numbers of "escape" shoots produced. Although the use of a wild-type "shooty" Agrobacterium strain allowed relatively high frequencies of beta-glucuronidase positive (GUS+) shoot ...
DOI:
10.1007/s002990050313

32. Factors influencing Agrobacterium mediated genetic transformation of kenaf

Author:
Herath, S.P.; Suzuki, T.; Hattori, K.
Source:
Plant cell, tissue, and organ culture 2005 v.82 no.2 pp. 201-206
ISSN:
0167-6857
Subject:
genetic transformation; gene transfer; cultivars; dose response; kenaf; transgenic plants; Agrobacterium radiobacter; genetic variation; transfer DNA; explants; gene expression; benzyladenine; micropropagation; Hibiscus cannabinus; enzyme activity; culture media; beta-glucuronidase
Abstract:
... As a first step in the development of a successful Agrobacterium tumefaciens mediated transformation method for kenaf, factors influencing the successful T-DNA integration and expression (as measured by the GUS expression) were investigated. Transformation was carried out using two kenaf cultivars and Agrobacterium strain EHA 105 carrying different vectors, plasmid pIG 121-Hm or pEC:gus. Pre-cultu ...

33. Genetic transformation of Begonia tuberhybrida by Ri rol genes

Author:
Kiyokawa, S.; Kikuchi, Y.; Kamada, H.; Harada, H.
Source:
Plant cell reports 1996 v.15 no.8 pp. 606-609
ISSN:
0721-7714
Subject:
corolla; Rhizobium rhizogenes; plasmids; genes; benzyladenine; shoots; dose response; phenotype; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; leaves; Begonia tuberhybrida; enzyme activity; culture media; Begonia cucullata; genetic transformation; gene transfer; dwarfing; kanamycin; transgenic plants; Begonia hiemalis; beta-glucuronidase
Abstract:
... We have developed an Agrobacterium-mediated transformation system for commercial Begonia species. The leaf explants of Begonia semperflorens, Begonia x hiemalis and B. tuberhybrida were inoculated with Agrobacterium tumefaciens LBA4404 harboring a binary vector pBI121 which contains rolA,B and C genes of an agropine type Ri plasmid (pRiA4b). Kanamycin resistant shoots of B. tuberhybrida were obtai ...
DOI:
10.1007/BF00232462
PubMed:
24178527

34. Genetic transformation of Cymbidium orchid by particle bombardment

Author:
Yang, J.; Lee, H.J.; Shin, D.H.; Oh, S.K.; Seon, J.H.; Paek, K.Y.; Han, K.H.
Source:
Plant cell reports 1999 v.18 no.12 pp. 978-984
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; in vitro culture; dose response; cell growth; transgenic plants; gene expression; genes; Cymbidium; benzyladenine; naphthaleneacetic acid; enzyme activity; culture media; beta-glucuronidase; shoots
Abstract:
... A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH20 ...
DOI:
10.1007/s002990050694

35. Genetic transformation of European chestnut

Author:
Seabra, R.C.; Pais, M.S.
Source:
Plant cell reports 1998 v.17 no.3 pp. 177-182
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; in vitro culture; kanamycin; hypocotyls; dose response; transgenic plants; Agrobacterium radiobacter; developmental stages; gene expression; histochemistry; genes; benzyladenine; micropropagation; Castanea sativa; enzyme activity; plant morphology; culture media; beta-glucuronidase
Abstract:
... Stable incorporation of the nptII gene into Castanea sativa Mill. has been achieved by Agrobacterium- mediated transformation. The transformation assays were performed by infecting wounded hypocotyls with a strain of Agrobacterium tumefaciens, LBA 4404 harbouring the plasmid p35SGUSINT. Although two schemes of selection were tested, many escapes were obtained. The best strategy to avoid this probl ...
DOI:
10.1007/s002990050374

36. Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration

Author:
Pena, L.; Cervera, M.; Juarez, J.; Navarro, A.; Pina, J.A.; Navarro, L.
Source:
Plant cell reports 1997 v.16 no.11 pp. 731-737
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; geneticin; kanamycin; dose response; transgenic plants; Citrus aurantiifolia; Agrobacterium radiobacter; explants; gene expression; histochemistry; micropropagation; enzyme activity; culture media; beta-glucuronidase; light
Abstract:
... We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck x Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We now report on the optimization of the cocultivation, regeneration and selection ...
DOI:
10.1007/s002990050311

37. Genetic transformation of the C3-C4 intermediate plant, Flaveria pubescens (Asteraceae)

Author:
Chu, C.C.; Qu, N.; Bassuner, B.; Bauwe, H.
Source:
Plant cell reports 1997 v.16 no.10 pp. 715-718
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; promoter regions; dose response; Agrobacterium radiobacter; benzyladenine; reporter genes; naphthaleneacetic acid; Flaveria pringlei; photosynthesis; culture media; beta-glucuronidase
Abstract:
... The dicot genus Flaveria (Asteraceae), besides species with C3 or C4 photosynthesis, contains taxa with a broad range of different states of transition between the two major photosynthetic types. We have developed a reproducible and efficient Agrobacterium-mediated method for the stable genetic transformation of the C3-C4 intermediate species F. pubescens. Fusion constructs of the reporter gene be ...
DOI:
10.1007/s002990050308

38. Herbicide safener-inducible gene expression in Arabidopsis thaliana

Author:
Veylder, L. de.; Montagu, M. van.; Inze, D.
Source:
Plant & cell physiology 1997 v.38 no.5 pp. 568-577
ISSN:
0032-0781
Subject:
herbicide safeners; developmental stages; branched chain amino acids; acetolactate synthase; genes; benzene; roots; dose response; gene expression; Arabidopsis thaliana; apical meristems; amino acid sequences; enzyme inhibitors; herbicide resistance; enzyme activity; histochemistry; transgenic plants; chlorsulfuron; beta-glucuronidase
Abstract:
... The potential use of a new chemical-inducible gene expression system in Arabidopsis thaliana has been examined. The system is based on the maize In2-2 promoter which is activated by benzenesulfonamide herbicide safeners. Plants transformed with the beta-glucuronidase (gus) reporter gene under the control of the In2-2 promoter were grown in the presence of different safeners and the induced GUS act ...
DOI:
10.1093/oxfordjournals.pcp.a029206
PubMed:
9210333

39. High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus)

Author:
Khan, M.R.; Rashid, H.; Ansar, M.; Chaudry, Z.
Source:
Plant cell, tissue, and organ culture 2003 v.75 no.3 pp. 223-231
ISSN:
0167-6857
Subject:
oil crops; benzyladenine; genes; Brassica napus; 2,4-D; shoots; dose response; hypocotyls; Agrobacterium radiobacter; gibberellic acid; explants; varieties; naphthaleneacetic acid; in vitro regeneration; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; silver nitrate; beta-glucuronidase
Abstract:
... Five different varieties of Brassica napus (Cyclone, Dunkled, Oscar, Rainbow and KS75) were tested for their regeneration response. Cyclone showed a very high frequency of regeneration (92%). The use of silver nitrate was a pre-requisite for efficient shoot regeneration. Hypocotyls were selected as the starting material for transformation experiments on the basis of high transient GUS expression. ...

40. High-efficiency transformation of Lycium barbarum mediated by Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis

Author:
Hu, Z.; Yang, J.; Guo, G.Q.; Zheng, G.C.
Source:
Plant cell reports 2002 v.21 no.3 pp. 233-237
ISSN:
0721-7714
Subject:
medicinal plants; micropropagation; neomycin; callus; genetic markers; dose response; Agrobacterium radiobacter; explants; leaves; Lycium; phosphotransferases (phosphomutases); culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; embryo (plant); somatic embryogenesis; reporter genes; beta-glucuronidase
Abstract:
... We have developed a reliable and high-frequency system of transformation and regeneration via somatic embryogenesis (SE) of Lycium barbarum. Leaf segments were co-cultivated with Agrobacterium tumefaciens EHA101 (pIG121Hm) carrying the neomycin phosphotransferase II gene as a selectable marker and an intron-beta-glucuronidase (GUS) gene as a reporter marker. On the medium for callus-induction, whi ...
DOI:
10.1007/s00299-002-0462-z

41. Highly efficient transformation and regeneration of aspen plants through shoot-bud formation in root culture

Author:
Tzfira, T.; Ben-Meir, H.; Vainstein, A.; Altman, A.
Source:
Plant cell reports 1996 v.15 no.8 pp. 555-571
ISSN:
0721-7714
Subject:
Rhizobium rhizogenes; plasmids; Populus tremula; neomycin; callus; shoots; genetic markers; dose response; stems; explants; clones; gene expression; adventitious roots; buds; genetic code; cell culture; phosphotransferases (phosphomutases); culture media; genetic transformation; kanamycin; transgenic plants; plant morphology; beta-glucuronidase
Abstract:
... The natural capacity of aspen (Populus tremula L.) roots for direct shoot-bud regeneration was harnessed to establish a highly efficient transformation and regeneration procedure that does not require a pre-selection stage on antibiotics. Aspen stem segments were transformed using wildtype Agrobacterium rhizogenes (LBA9402) with the binary p35SGUSINT plasmid carrying the genes coding for beta-gluc ...
DOI:
10.1007/BF00232452

42. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

43. Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus

Author:
Cho, H.J.; Widholm, J.M.
Source:
Plant cell, tissue, and organ culture 2002 v.69 no.3 pp. 259-269
ISSN:
0167-6857
Subject:
developmental stages; Rhizobium rhizogenes; genes; benzyladenine; 2,4-D; shoots; in vitro culture; dose response; gene expression; in vitro regeneration; kinetin; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; transgenic plants; Astragalus sinicus; plant morphology; genetic vectors; beta-glucuronidase
Abstract:
... An improved protocol for shoot regeneration from hairy roots transformed by Agrobacterium rhizogenes of the legume species Astragalus sinicus (Chinese milk vetch) has been developed. The A. rhizogenes strain DC-AR2 harboring the binary vector pBI121 which carries the uidA gene encoding beta-glucuronidase activity and the kanamycin resistance gene nptII, was used to transform cut ends of plantlet h ...

44. Interactive Effects of Cytokinins, Light, and Sucrose on the Phenotypes and the Syntheses of Anthocyanins and Lignins in Cytokinin Overproducing Transgenic Arabidopsis

Author:
Jianchun Guo; Xinwen Hu; Ruijun Duan
Source:
Journal of plant growth regulation 2005 v.24 no.2 pp. 93-101
ISSN:
0721-7595
Subject:
Arabidopsis thaliana; seedlings; in vitro culture; anthocyanins; lignin; biosynthesis; beta-glucuronidase; enzyme activity; culture media; cytokinins; sucrose; dose response; light intensity; phenotypic correlation; genes; gene expression; transcription (genetics); transgenic plants; wild relatives; genetic variation
Abstract:
... Elevated cytokinins levels in vivo resulted in both enlarged and retarded growth phenotypes. Cytokinin levels were similar in both phenotypic groups. The ratio of the two phenotypes could be altered by changing the sucrose concentration in MS medium or the light intensity. Increased cytokinins in ipt:: GUS activated Arabidopsis induced the syntheses of anthocyanins and lignins, while light speeded ...
DOI:
10.1007/s00344-005-0005-2

45. Intestinal Health Benefits of the Water-Soluble Carbohydrate Concentrate of Wild Grape (Vitis thunbergii) in Hamsters

Author:
Huang Ya-Ling; Chu Hui-Fang; Dai Fan-Jhen; Yu Tzu-Yi; Chau Chi-Fai
Source:
Journal of agricultural and food chemistry 2012 v.60 no.19 pp. 4854-4858
ISSN:
1520-5118
Subject:
Vitis; ammonia; beta-glucosidase; beta-glucuronidase; diet; dose response; fructose; gastrointestinal transit; glucose; grapes; hamsters; hemicellulose; intestinal mucosa; short chain fatty acids; water content
Abstract:
... The dose–response relationship of the water-soluble carbohydrate concentrate (WSCC) from wild grape (Vitis thunbergii Sieb. & Zucc.) on intestinal health was investigated in this study. WSCC contained carbohydrates up to 71.9 g/100 g, including arabinose-rich pectic polysaccharide, hemicelluloses, glucose, and fructose. The consumption of WSCC (0.5 and 1.5 g/100 g of diet) effectively (P < 0.05) s ...
DOI:
10.1021/jf300942p
PubMed:
22546171

46. Lactobacillus- and bifidobacterium-mediated antigenotoxicity in the colon of rats

Author:
Pool-Zobel, B.L.; Neudecker, C.; Domizlaff, I.; Ji, S.; Schillinger, U.; Rumney, C.; Moretti, M.; Vilarini, I.; Scassellati-Sforzolini, R.; Rowland, I.
Source:
Nutrition and cancer 1996 v.26 no.3 pp. 365-380
ISSN:
0163-5581
Subject:
genotoxicity; heat treatment; extracts; Lactobacillus acidophilus; Lactobacillus gasseri; Bifidobacterium longum; acetone; Bifidobacterium breve; glutathione; dose response; Weissella confusa; cell walls; glutathione transferase; DNA; cytoplasm; Streptococcus thermophilus; beta-glucosidase; colon; strain differences; inactivation; cytochrome P-450; N-methyl-N-nitrosoguanidine; duration; sodium chloride; rats; enzyme activity; acetic acid; liver; freeze drying; lactic acid bacteria; oxidoreductases; cultured cells; animal models; 1,2-dimethylhydrazine; beta-glucuronidase
Abstract:
... Lactic acid bacteria (LAB) are proposed to have several beneficial effects, including the inactivation of carcinogens. We have studied the potential of Lactobacillus acidophilus (from a commercially available yogurt), Lactobacillus gasseri (P79), Lactobacillus confusus (DSM 20196), Streptococcus thermophilus (NCIM 50083), Bifidobacterium breve and Bifidobacterium longum (from human infant stool) t ...
DOI:
10.1080/01635589609514492
PubMed:
8910918

47. Optimization of biolistic bombardment parameters for Dendrobium Sonia 17 calluses using GFP and GUS as the reporter system

Author:
Tee, C.S.; Maziah, M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.1 pp. 77-89
ISSN:
0167-6857
Subject:
ornamental plants; genetic transformation; plant breeding; green fluorescent protein; in vitro culture; biolistics; Dendrobium; hygromycin B; dose response; transgenic plants; gene expression; reporter genes; micropropagation; enzyme activity; in vitro regeneration; culture media; beta-glucuronidase; callus
Abstract:
... Genetic transformation system of Dendrobium Sonia 17 was optimized using green fluorescent protein (GFP) and beta-glucuronidase (GUS) gene as the reporter systems. The 35S-sgfp-TYG-nos (p35S) and pSMDFR, carrying sgfp and gusA gene, respectively, were co-bombarded into the calluses. Parameters optimized were acceleration pressure, target distance, gold particle size, pre-bombardment cultured time, ...

48. Organ-specific expression of beta-glucuronidase activity driven by the Arabidopsis heat-shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia

Author:
Moriwaki, M.; Yamakawa, T.; Washino, T.; Kodama, T.; Igarashi, Y.
Source:
Plant cell reports 1999 v.19 no.1 pp. 92-95
ISSN:
0721-7714
Subject:
genes; micropropagation; Nicotiana plumbaginifolia; promoter regions; in vitro culture; dose response; gene expression; leaves; Arabidopsis thaliana; temperature; enzyme activity; culture media; heat shock proteins; genetic transformation; gene transfer; transgenic plants; plant growth substances; stress response; flowers; beta-glucuronidase
Abstract:
... The expression of the Arabidopsis heat-shock protein (HSP) 18.2 promoter beta-D-glucuronidase (GUS) chimera gene was studied in various organs of the transgenic Nicotiana plumbaginifolia during the recovery phase at normal temperatures (20-22 degrees C) following heat-shock (HS) treatment. The optimum HS temperature for GUS activity in the anthers, petals and capsules was 42 degrees C, but in imma ...
DOI:
10.1007/s002990050716

49. Particle bombardment, a method for gene transfer in marigold

Author:
Vanegas, P.E.; Valdez-Morales, M.; Valverde, M.E.; Cruz-Hernandez, A.; Paredes-Lopez, O.
Source:
Plant cell, tissue, and organ culture 2006 v.84 no.3 pp. 359-363
ISSN:
0167-6857
Subject:
particles; plasmids; genes; micropropagation; callus; organogenesis; in vitro culture; dose response; gene expression; in vitro regeneration; leaves; ornamental plants; culture media; genetic transformation; biolistics; kanamycin; transgenic plants; genetic stability; Tagetes erecta; beta-glucuronidase
Abstract:
... Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue w ...
DOI:
10.1007/s11240-005-9030-x

50. Production of transgenic gentian plants by particle bombardment of suspension-culture cells

Author:
Hosokawa, K.; Matsuki, R.; Oikawa, Y.; Yamamura, S.
Source:
Plant cell reports 2000 v.19 no.5 pp. 454-458
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; dose response; beta-glucuronidase; transgenic plants; enzyme activity; culture media; gene expression; phosphotransferases (phosphomutases); genes; naphthaleneacetic acid; Gentiana; histochemistry; cell suspension culture
DOI:
10.1007/s002990050755