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You searched for: Subject term dose response Remove constraint Subject term: dose response Subject term beta-glucuronidase Remove constraint Subject term: beta-glucuronidase
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1. Application of metabonomic strategy to discover an unreported active ingredient in LiuWeiDiHuang pills suppressing beta-glucuronidase

Author:
Baogang Xie; Zhirong Zhang; Tao Gong; Ningning Zhang; Huiyun Wang; Huiqing Zou
Source:
Analytical and bioanalytical chemistry 2015 v.407 no.2 pp. 609-614
ISSN:
1618-2642
Subject:
Oriental traditional medicine; active ingredients; beta-glucuronidase; bioactive properties; daidzein; discriminant analysis; dose response; drugs; genistein; high performance liquid chromatography; least squares; liver; mass spectrometry; metabolomics; nuclear magnetic resonance spectroscopy; rats; screening; urine
Abstract:
... Identification of the bioactive ingredient from traditional Chinese medicine (TCM) remains a challenging task by traditional approach that focuses on chemical isolation coupled with biological activity screening. Here, we present a metabonomics-based approach for bioactive ingredient discovery in LiuWeiDiHuang pills (LWPs). First, a non-targeted high-performance liquid chromatography ultraviolet ( ...
DOI:
10.1007/s00216-014-8253-2

2. Andalusol, a Diterpenoid with anti-inflammatory Activity from Siderits foetens Clemen

Author:
A. Navarro; B. de las Heras
Source:
Zeitschrift für Naturforschung 2014 v.52 no.11-12 pp. 844-849
ISSN:
1865-7125
Subject:
Sideritis; acetates; acetone; anti-inflammatory activity; beta-glucuronidase; calcium ionophores; carrageenan; diterpenoids; dose response; histamine; lactate dehydrogenase; mast cells; myeloperoxidase; neutrophils; rats; topical application; toxicity
Abstract:
... The anti-inflammatory activity of andalusol (ent-13(16),14-labdadiene-6α,8α,18-triol), a diterpenoid obtained from the acetone extract of Sideritis foetens Clemen, has been investigated. This compound was able to inhibit acute inflammatory processes induced by carrageenan or 12-O -tetradecanoylphorbol acetate (TPA) after oral or topical administration. Quantitation of the neutrophil specific marke ...
DOI:
10.1515/znc-1997-11-1219

3. Validation study on urinary biomarkers of exposure for aflatoxin B₁, ochratoxin A, fumonisin B₁, deoxynivalenol and zearalenone in piglets

Author:
S. Gambacorta; H. Solfrizzo; A. Visconti; S. Powers; A.M. Cossalter; P. Pinton; I.P. Oswald
Source:
World mycotoxin journal 2013 v.6 no.3 pp. 299-308
ISSN:
1875-0796
Subject:
aflatoxin B1; aflatoxin M1; beta-glucuronidase; biomarkers; cages; correlation; deoxynivalenol; dose response; fumonisin B1; ochratoxin A; piglets; toxigenic strains; urine; zearalenone
Abstract:
... The multi-biomarker approach was used to validate urinary biomarkers in piglets administered boluses contaminated with mixtures of deoxynivalenol (DON), aflatoxin B₁ (AFB₁), fumonisin B₁ (FB₁), zearalenone (ZEA) and ochratoxin A (OTA) at different concentrations. Boluses contaminated with mycotoxins were prepared by slurrying and freezedrying feed material fortified with culture extracts of select ...
DOI:
10.3920/WMJ2013.1549

4. Intestinal Health Benefits of the Water-Soluble Carbohydrate Concentrate of Wild Grape (Vitis thunbergii) in Hamsters

Author:
Huang Ya-Ling; Chu Hui-Fang; Dai Fan-Jhen; Yu Tzu-Yi; Chau Chi-Fai
Source:
Journal of agricultural and food chemistry 2012 v.60 no.19 pp. 4854-4858
ISSN:
1520-5118
Subject:
Vitis; ammonia; beta-glucosidase; beta-glucuronidase; diet; dose response; fructose; gastrointestinal transit; glucose; grapes; hamsters; hemicellulose; intestinal mucosa; short chain fatty acids; water content
Abstract:
... The dose–response relationship of the water-soluble carbohydrate concentrate (WSCC) from wild grape (Vitis thunbergii Sieb. & Zucc.) on intestinal health was investigated in this study. WSCC contained carbohydrates up to 71.9 g/100 g, including arabinose-rich pectic polysaccharide, hemicelluloses, glucose, and fructose. The consumption of WSCC (0.5 and 1.5 g/100 g of diet) effectively (P < 0.05) s ...
DOI:
10.1021/jf300942p
PubMed:
22546171

5. Differential immune responses of the green neon shrimp (Neocaridina denticulate) to dipropyl phthalate

Author:
Hung-Hung Sung; Yi-Hsiu Lin; Chun-Yi Hsiao
Source:
Fish & shellfish immunology 2011 v.31 no.3 pp. 511-515
ISSN:
1050-4648
Subject:
Aeromonas veronii; acid phosphatase; bacterial infections; beta-glucuronidase; dose response; hemocyanin; immune response; messenger RNA; monophenol monooxygenase; mortality; phthalates; shrimp; sublethal effects; superoxide dismutase
Abstract:
... This study set out to understand the sublethal effect of xenobiotic phthalate esters (PAEs) on the relationship between the susceptibility of shrimp to bacterial infection and the immune response of the shrimp. Neocaridina denticulate were exposed to different concentrations of the PAE dipropyl phthalate (DPrP), and mortality and six immune parameters were measured on days 1, 3, 5, and 10 after ex ...
DOI:
10.1016/j.fsi.2011.06.021

6. Effects of carrot and tomato juice consumption on faecal markers relevant to colon carcinogenesis in humans

Author:
Schnabele, K.; Briviba, K.; Bub, A.; Roser, S.; Pool-Zobel, B.L.; Rechkemmer, G.
Source:
British journal of nutrition 2008 v.99 no.3 pp. 606-613
ISSN:
0007-1145
Subject:
disease prevention; alpha-carotene; short chain fatty acids; carcinogenesis; dose response; biomarkers; lycopene; feces; beta-carotene; tomato juice; carrot juice; beta-glucosidase; humans; cytotoxicity; adenocarcinoma; colorectal neoplasms; cell proliferation; food intake; dosage; cultured cells; pH; bile acids; beta-glucuronidase
DOI:
10.1017/S0007114507819143
PubMed:
18254985

7. Aryl hydrocarbon receptor (AhR)-mediated reporter gene expression systems in transgenic tobacco plants

Author:
Kodama, Susumu; Okada, Kumiko; Inui, Hideyuki; Ohkawa, Hideo
Source:
Planta 2007 v.227 no.1 pp. 37-45
ISSN:
0032-0935
Subject:
beta-glucuronidase; bioassays; dioxins; dose response; gene expression; herpes simplex; indigo; leaves; ligands; mice; reporter genes; soaking; tobacco; transgenic plants; viruses
Abstract:
... In mammals, the aryl hydrocarbon receptor (AhR) mediates expression of certain genes, including CYP1A1, in response to exposure to dioxins and related compounds. We have constructed a mouse AhR-mediated gene expression systems for a β-glucuronidase (GUS) reporter gene consisting of an AhR, an AhR nuclear translocator (Arnt), and a xenobiotic response element (XRE)-driven promoter in transgenic tob ...
DOI:
10.1007/s00425-007-0592-1
PubMed:
17879099

8. Epinephrine enhances lysosomal enzyme delivery across the blood-brain barrier by up-regulation of the mannose 6-phosphate receptor

Author:
Urayama, Akihiko; Grubb, Jeffrey H.; Banks, William A.; Sly, William S.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.31 pp. 12873-12878
ISSN:
0027-8424
Subject:
adults; beta-glucuronidase; blood-brain barrier; brain; dose response; epinephrine; glycosaminoglycans; mannose; mice; mucopolysaccharidosis; neonates; parenchyma (animal tissue); therapeutics
Abstract:
... Delivering therapeutic levels of lysosomal enzymes across the blood-brain barrier (BBB) has been a pivotal issue in treating CNS storage diseases, including the mucopolysaccharidoses. An inherited deficiency of β-glucuronidase (GUS) causes mucopolysaccharidosis type VII that is characterized by increased systemic and CNS storage of glycosaminoglycans. We previously showed that the neonate uses the ...
DOI:
10.1073/pnas.0705611104
PubMed:
17646643
PubMed Central:
PMC1937559

9. Particle bombardment, a method for gene transfer in marigold

Author:
Vanegas, P.E.; Valdez-Morales, M.; Valverde, M.E.; Cruz-Hernandez, A.; Paredes-Lopez, O.
Source:
Plant cell, tissue, and organ culture 2006 v.84 no.3 pp. 359-363
ISSN:
0167-6857
Subject:
particles; plasmids; genes; micropropagation; callus; organogenesis; in vitro culture; dose response; gene expression; in vitro regeneration; leaves; ornamental plants; culture media; genetic transformation; biolistics; kanamycin; transgenic plants; genetic stability; Tagetes erecta; beta-glucuronidase
Abstract:
... Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue w ...
DOI:
10.1007/s11240-005-9030-x

10. Agrobacterium tumefaciens-mediated transformation of highly regenerable cell suspension cultures in Dianthus acicularis

Author:
Shiba, T.; Mii, M.
Source:
Journal of horticultural science & biotechnology 2005 v.80 no.4 pp. 393-398
ISSN:
1462-0316
Subject:
hygromycin B; micropropagation; callus; dose response; Agrobacterium radiobacter; explants; gene expression; in vitro regeneration; leaves; ornamental plants; beta-glucuronidase; cut flowers; enzyme activity; Dianthus; culture media; genetic transformation; gene transfer; transgenic plants; cell suspension culture; acetosyringone

11. Calluses initiated from thin mature embryo fragments are suitable targets for wheat transformation as assessed by long-term GUS expression studies

Author:
Delporte, F.; Li, S.; Jacquemin, J.M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 139-149
ISSN:
0167-6857
Subject:
genetic transformation; dose response; biolistics; transgenic plants; embryo (plant); Agrobacterium radiobacter; explants; wheat; gene expression; Triticum aestivum; 2,4-D; micropropagation; enzyme activity; in vitro regeneration; culture media; beta-glucuronidase; callus
Abstract:
... Microprojectile- or Agrobacterium-mediated DNA delivery into calluses initiated from immature embryos has proven to be effective in transforming wheat. Yet, obtaining a large number of high quality immature embryos throughout the year is a laborious and delicate process. To circumvent these limitations, we propose an alternative technique applying the particle bombardment technology to calluses de ...

12. Optimization of biolistic bombardment parameters for Dendrobium Sonia 17 calluses using GFP and GUS as the reporter system

Author:
Tee, C.S.; Maziah, M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.1 pp. 77-89
ISSN:
0167-6857
Subject:
ornamental plants; genetic transformation; plant breeding; green fluorescent protein; in vitro culture; biolistics; Dendrobium; hygromycin B; dose response; transgenic plants; gene expression; reporter genes; micropropagation; enzyme activity; in vitro regeneration; culture media; beta-glucuronidase; callus
Abstract:
... Genetic transformation system of Dendrobium Sonia 17 was optimized using green fluorescent protein (GFP) and beta-glucuronidase (GUS) gene as the reporter systems. The 35S-sgfp-TYG-nos (p35S) and pSMDFR, carrying sgfp and gusA gene, respectively, were co-bombarded into the calluses. Parameters optimized were acceleration pressure, target distance, gold particle size, pre-bombardment cultured time, ...

13. Regeneration from leaf-base explants of Lolium perenne L. and Lolium multiflorum L

Author:
Newell, C.A.; Gray, J.C.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 233-237
ISSN:
0167-6857
Subject:
lawns and turf; genes; benzyladenine; micropropagation; 2,4-D; callus; dose response; explants; gene expression; embryogenesis; in vitro regeneration; leaves; Lolium perenne; enzyme activity; culture media; Lolium multiflorum; genetic transformation; forage crops; biolistics; transgenic plants; beta-glucuronidase
Abstract:
... The regenerability of Lolium perenne and Lolium multiflorum was investigated using leaf-base sections excised from plantlets grown in vitro. Young leaf bases were cut into three sections each 2 mm in length, the lowest 0-2 mm section adjacent to, but not including, the apical meristem. Callus development followed by embryogenesis occurred only in the lowest 2 mm leaf-base sections of L. perenne cv ...

14. Agrobacterium-mediated transformed transgenic triploid bermudagrass (Cynodon dactylon x C. transvaalensis) plants are highly resistant to the glufosinate herbicide Liberty

Author:
Hu, F.; Zhang, L.; Wang, X.; Ding, J.; Wu, D.
Source:
Plant cell, tissue, and organ culture 2005 v.83 no.1 pp. 13-19
ISSN:
0167-6857
Subject:
Cynodon dactylon; glufosinate; genes; benzyladenine; micropropagation; 2,4-D; Cynodon transvaalensis; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; casein hydrolysates; naphthaleneacetic acid; turf grasses; herbicide resistance; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; abscisic acid; beta-glucuronidase; acetosyringone
Abstract:
... Glufosinate resistance gene isolated from Streptomyces hygromicinroscopicus (bar) that confers the resistance of herbicide Liberty, a broad-spectrum grass and broadleaf contact herbicide widely used for weed control, was introduced into triploid bermudagrass by Agrobacterium-mediated transformation. Embryogenic calluses derived from stolonous nodal segment were co-cultured with the disarmed strain ...

15. Effects of antibiotics on the elimination of Agrobacterium tumefaciens from loblolly pine (Pinus taeda) zygotic embryo explants and on transgenic plant regeneration

Author:
Tang, W.; Luo, H.; Newton, R.J.
Source:
Plant cell, tissue, and organ culture 2004 v.79 no.1 pp. 71-81
ISSN:
0167-6857
Subject:
benzyladenine; micropropagation; Pinus taeda; 2,4-D; bacterial contamination; dose response; Agrobacterium radiobacter; explants; zygote; forest trees; culture media; genetic transformation; gene transfer; transgenic plants; embryo (plant); indole butyric acid; reporter genes; beta-glucuronidase; carbenicillin
Abstract:
... Three antibiotics were evaluated for their effects on the elimination of Agrobacterium tumefaciens during the genetic transformation of loblolly pine (Pinus taeda L.) using mature zygotic embryos as targets. Agrobacterium tumefaciens strains, EHA105, GV3101, and LBA 4404, all harbouring the plasmid pCAMBIA1301, which carries the selectable marker gene, hygromycin phosphotransferase (hpt) controlle ...

16. Effects of antioxidants on the plant regeneration and GUS expressive frequency of peanut (Arachis hypogaea) explants by Agrobacterium tumefaciens

Author:
Zheng, Q..; Ju, B.; Liang, L.; Xiao, X.
Source:
Plant cell, tissue, and organ culture 2005 v.81 no.1 pp. 83-90
ISSN:
0167-6857
Subject:
peanuts; glutathione; dose response; malondialdehyde; hydrogen peroxide; Agrobacterium radiobacter; explants; Arachis hypogaea; in vitro regeneration; superoxide dismutase; selenites; enzyme activity; culture media; ascorbic acid; genetic transformation; gene transfer; catalase; transgenic plants; beta-glucuronidase

17. Factors influencing Agrobacterium mediated genetic transformation of kenaf

Author:
Herath, S.P.; Suzuki, T.; Hattori, K.
Source:
Plant cell, tissue, and organ culture 2005 v.82 no.2 pp. 201-206
ISSN:
0167-6857
Subject:
genetic transformation; gene transfer; cultivars; dose response; kenaf; transgenic plants; Agrobacterium radiobacter; genetic variation; transfer DNA; explants; gene expression; benzyladenine; micropropagation; Hibiscus cannabinus; enzyme activity; culture media; beta-glucuronidase
Abstract:
... As a first step in the development of a successful Agrobacterium tumefaciens mediated transformation method for kenaf, factors influencing the successful T-DNA integration and expression (as measured by the GUS expression) were investigated. Transformation was carried out using two kenaf cultivars and Agrobacterium strain EHA 105 carrying different vectors, plasmid pIG 121-Hm or pEC:gus. Pre-cultu ...

18. Interactive Effects of Cytokinins, Light, and Sucrose on the Phenotypes and the Syntheses of Anthocyanins and Lignins in Cytokinin Overproducing Transgenic Arabidopsis

Author:
Jianchun Guo; Xinwen Hu; Ruijun Duan
Source:
Journal of plant growth regulation 2005 v.24 no.2 pp. 93-101
ISSN:
0721-7595
Subject:
Arabidopsis thaliana; seedlings; in vitro culture; anthocyanins; lignin; biosynthesis; beta-glucuronidase; enzyme activity; culture media; cytokinins; sucrose; dose response; light intensity; phenotypic correlation; genes; gene expression; transcription (genetics); transgenic plants; wild relatives; genetic variation
Abstract:
... Elevated cytokinins levels in vivo resulted in both enlarged and retarded growth phenotypes. Cytokinin levels were similar in both phenotypic groups. The ratio of the two phenotypes could be altered by changing the sucrose concentration in MS medium or the light intensity. Increased cytokinins in ipt:: GUS activated Arabidopsis induced the syntheses of anthocyanins and lignins, while light speeded ...
DOI:
10.1007/s00344-005-0005-2

19. Agrobacterium-mediated genetic transformation of a Dendrobium orchid

Author:
Men, S.; Ming, X.; Liu, R.; Wei, C.; Li, Y.
Source:
Plant cell, tissue, and organ culture 2003 v.75 no.1 pp. 63-71
ISSN:
0167-6857
Subject:
hygromycin B; benzyladenine; micropropagation; genetic markers; marker-assisted selection; Dendrobium; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; ornamental plants; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; transgenic plants; rapid methods; corms; reporter genes; genetic vectors; beta-glucuronidase; acetosyringone
Abstract:
... A protocol was developed to obtain stable transgenic orchids (Dendrobium nobile) via Agrobacterium-mediated transformation of protocorm-like bodies (PLBs). Agrobacterium tumefaciens strains AGL1 and EHA105 were used, with each containing a binary vector pCAMBIA1301 with the hpt gene as a selectable marker for hygromycin resistance and an intron-containing β-glucuronidase gene (gus-int) as a report ...

20. Agrobacterium-mediated transient transformation of annatto (Bixa orellana) hypocotyls with the gus reporter gene

Author:
Zaldivar-Cruz, J.M.; Ballina-Gomez, H.; Guerrero-Rodriguez, C.; Aviles-Berzunza, E.; Godoy-Hernandez, G.C.
Source:
Plant cell, tissue, and organ culture 2003 v.73 no.3 pp. 281-284
ISSN:
0167-6857
Subject:
food coloring; benzyladenine; dye plants; in vitro culture; dose response; hypocotyls; Agrobacterium radiobacter; gene expression; cultivars; genetic improvement; enzyme activity; histochemistry; culture media; genetic transformation; gene transfer; Bixa orellana; transgenic plants; reporter genes; genetic variation; beta-glucuronidase
Abstract:
... Hypocotyls from annatto seedlings, were inoculated with Agrobacterium tumefaciens harboring a binary vector, pBI.121 or pCAMBIA2301, containing the β-glucuronidase (gus) gene. Histochemical GUS assay of infected hypocotyls from two annatto varieties showed transient gus gene expression between 3 and 12 days after inoculation. ...

21. Cytokinin oxidase gene expression in maize is localized to the vasculature, and is induced by cytokinins, abscisic acid, and abiotic stress

Author:
Brugiere, N.; Jiao, S.; Hantke, S.; Zinselmeier, C.; Roessler, J.A.; Niu, X.O.; Jones, R.J.; Habben, J.E.
Source:
Plant physiology 2003 v.132 no.3 pp. 1228-1240
ISSN:
0032-0889
Subject:
alleles; seedlings; roots; plant proteins; coleoptiles; dose response; open reading frames; tissue distribution; water stress; gene expression; heat stress; isopentenyladenine; Zea mays; plant development; messenger RNA; corn; vascular bundles; grain crops; transgenic plants; oxidoreductases; cytokinins; reporter genes; abscisic acid; beta-glucuronidase
DOI:
10.1104/pp.102.017707
PubMed:
12857805
PubMed Central:
PMC167063

22. Extracellular ATP inhibits root gravitropism at concentrations that inhibit polar auxin transport

Author:
Tang, W.; Brady, S.R.; Sun, Y.; Muday, G.K.; Roux, S.J.
Source:
Plant physiology 2003 v.131 no.1 pp. 147-154
ISSN:
0032-0889
Subject:
root tips; seedling growth; 2,4-D; dose response; naphthaleneacetic acid; plasma membrane; Arabidopsis thaliana; Zea mays; corn; adenosine triphosphate; translocation (plant physiology); culture media; indole acetic acid; food crops; gravitropism; transgenic plants; physiological transport; reporter genes; plant morphology; beta-glucuronidase
Abstract:
... Raising the level of extracellular ATP to mM concentrations similar to those found inside cells can block gravitropism of Arabidopsis roots. When plants are grown in Murashige and Skoog medium supplied with 1 mM ATP, their roots grow horizontally instead of growing straight down. Medium with 2 mM ATP induces root curling, and 3 mM ATP stimulates lateral root growth. When plants are transferred to ...
PubMed:
12529523
PubMed Central:
PMC166795

23. High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus)

Author:
Khan, M.R.; Rashid, H.; Ansar, M.; Chaudry, Z.
Source:
Plant cell, tissue, and organ culture 2003 v.75 no.3 pp. 223-231
ISSN:
0167-6857
Subject:
oil crops; benzyladenine; genes; Brassica napus; 2,4-D; shoots; dose response; hypocotyls; Agrobacterium radiobacter; gibberellic acid; explants; varieties; naphthaleneacetic acid; in vitro regeneration; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; silver nitrate; beta-glucuronidase
Abstract:
... Five different varieties of Brassica napus (Cyclone, Dunkled, Oscar, Rainbow and KS75) were tested for their regeneration response. Cyclone showed a very high frequency of regeneration (92%). The use of silver nitrate was a pre-requisite for efficient shoot regeneration. Hypocotyls were selected as the starting material for transformation experiments on the basis of high transient GUS expression. ...

24. Sucrose level influences micropropagation and gene delivery into leaves from in vitro propagated highbush blueberry shoots

Author:
Cao, X.; Fordham, I.; Douglass, L.; Hammerschlag, F.
Source:
Plant cell, tissue, and organ culture 2003 v.75 no.3 pp. 255-259
ISSN:
0167-6857
Subject:
in vitro regeneration; genetic transformation; gene transfer; cultivars; dose response; sucrose; Agrobacterium radiobacter; transgenic plants; explants; gene expression; leaves; genes; genetic variation; blueberries; culture media; beta-glucuronidase; Vaccinium corymbosum; shoots
Abstract:
... In an effort to increase in vitro blueberry (Vaccinium corymbosum L.) shoot production without negatively impacting subsequent genetic engineering experiments, studies were conducted to examine the effects of sucrose concentration in the propagation medium on shoot proliferation and on the transfer of an intron-containing β-glucuronidase (GUS) gene into leaf explants from the propagated shoots. Nu ...

25. High-efficiency transformation of Lycium barbarum mediated by Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis

Author:
Hu, Z.; Yang, J.; Guo, G.Q.; Zheng, G.C.
Source:
Plant cell reports 2002 v.21 no.3 pp. 233-237
ISSN:
0721-7714
Subject:
medicinal plants; micropropagation; neomycin; callus; genetic markers; dose response; Agrobacterium radiobacter; explants; leaves; Lycium; phosphotransferases (phosphomutases); culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; embryo (plant); somatic embryogenesis; reporter genes; beta-glucuronidase
Abstract:
... We have developed a reliable and high-frequency system of transformation and regeneration via somatic embryogenesis (SE) of Lycium barbarum. Leaf segments were co-cultivated with Agrobacterium tumefaciens EHA101 (pIG121Hm) carrying the neomycin phosphotransferase II gene as a selectable marker and an intron-beta-glucuronidase (GUS) gene as a reporter marker. On the medium for callus-induction, whi ...
DOI:
10.1007/s00299-002-0462-z

26. Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus

Author:
Cho, H.J.; Widholm, J.M.
Source:
Plant cell, tissue, and organ culture 2002 v.69 no.3 pp. 259-269
ISSN:
0167-6857
Subject:
developmental stages; Rhizobium rhizogenes; genes; benzyladenine; 2,4-D; shoots; in vitro culture; dose response; gene expression; in vitro regeneration; kinetin; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; transgenic plants; Astragalus sinicus; plant morphology; genetic vectors; beta-glucuronidase
Abstract:
... An improved protocol for shoot regeneration from hairy roots transformed by Agrobacterium rhizogenes of the legume species Astragalus sinicus (Chinese milk vetch) has been developed. The A. rhizogenes strain DC-AR2 harboring the binary vector pBI121 which carries the uidA gene encoding beta-glucuronidase activity and the kanamycin resistance gene nptII, was used to transform cut ends of plantlet h ...

27. The integration of a major hepatitis B virus gene into cell-cycle synchronized carrot cell suspension cultures and its expression in regenerated carrot plants

Author:
Imani, J.; Berting, A.; Nitsche, S.; Schaefer, S.; Gerlich, W.H.; Neumann, K.H.
Source:
Plant cell, tissue, and organ culture 2002 v.71 no.2 pp. 157-164
ISSN:
0167-6857
Subject:
Hepatitis B virus; genes; 2,4-D; roots; callus; dose response; Agrobacterium radiobacter; Daucus carota; gene expression; naphthaleneacetic acid; carrots; in vitro regeneration; beta-glucuronidase; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; somatic embryogenesis; cultured cells; viral proteins; cell cycle; cell suspension culture
Abstract:
... The percentage of cells successfully transformed by Agrobacteria is usually very low (not more than 10% and usually much less; Thomas et al., 1989). In this report we show that in carrot (Daucus carota L., ssp. sativus) cell suspensions transformation efficiency was strongly improved by using cell cycle synchronized cells. Fluorodesoxyuridine (FDU) was added for 24 h to inhibit thymidine synthesis ...

28. Agrobacterium-mediated transformation of a doubled haploid line of cabbage

Author:
Tsukazaki, H.; Kuginuki, Y.; Aida, R.; Suzuki, T.
Source:
Plant cell reports 2002 v.21 no.3 pp. 257-262
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; dose response; hypocotyls; haploidy; transgenic plants; Agrobacterium radiobacter; explants; gene expression; acetosyringone; genes; micropropagation; Brassica oleracea var. capitata; inheritance (genetics); culture media; beta-glucuronidase; shoots
Abstract:
... Doubled haploid lines, which have high levels of genetic uniformity, are suitable for physiological or genetic studies. We established a transformation system using a doubled haploid line of cabbage. Hypocotyl explants that had been precultured for 3 days on Murashige and Skoog medium containing 50 micromolar acetosyringone were inoculated and cocultured with Agrobacterium tumefaciens strain LBA44 ...
DOI:
10.1007/s00299-002-0506-4

29. Developmental phases and STM expression during Arabidopsis shoot organogenesis

Author:
Zhao, Q.H.; Fisher, R.; Auer, C.
Source:
Plant growth regulation 2002 v.37 no.3 pp. 223-231
ISSN:
0167-6903
Subject:
adenosine; developmental stages; genes; benzyladenine; shoot meristems; roots; promoter regions; callus; organogenesis; dose response; explants; gene expression; isopentenyladenine; naphthaleneacetic acid; Arabidopsis thaliana; enzyme activity; culture media; tissue culture; zeatin; cotyledons; plant morphology; beta-glucuronidase
Abstract:
... Shoot organogenesis in Arabidopsis thaliana was studied with regard to the timing of key developmental phases and expression of the SHOOTMERISTEMLESS (STM) gene. Shoot regeneration in the highly organogenic ecotype C24 was affected by explant type and age. The percentage of C24 cotyledon explants producing shoots decreased from 90% to 26% when donor seedlings were more than 6 d old, but 96% of roo ...

30. Stable transformation of Pinus radiata embryogenic tissue by Agrobacterium tumefaciens

Author:
Cerda, F.; Aquea, F.; Gebauer, M.; Medina, C.; Arce-Johnson, P.
Source:
Plant cell, tissue, and organ culture 2002 v.70 no.3 pp. 251-257
ISSN:
0167-6857
Subject:
Pinus radiata; developmental stages; genes; benzyladenine; micropropagation; 2,4-D; in vitro culture; dose response; Agrobacterium radiobacter; zygote; in vitro regeneration; somatic embryos; forest trees; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; abscisic acid; plant morphology; beta-glucuronidase
Abstract:
... Embryogenic cultures from immature zygotic embryos of Pinus radiata seeds were established on semisolid proliferation medium with 2,4-D and BAP. Growing embryogenic masses containing embryonal cells and suspensor cells were subcultured on this media every 2 weeks. After 10 weeks, embryogenic masses (1.5 cm diameter) were transferred to a maturation medium containing ABA. Fully developed somatic em ...

31. An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.)

Author:
D. Das; M. Reddy; K. Upadhyaya; S. Sopory
Source:
Plant cell reports 2002 v.20 no.11 pp. 999-1005
ISSN:
0721-7714
Subject:
grapes; developmental stages; light intensity; benzyladenine; micropropagation; cell differentiation; 2,4-D; callus; dose response; Agrobacterium radiobacter; gibberellic acid; explants; genotype; Vitis vinifera; leaves; cultivars; histology; temperature; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; embryo (plant); indole butyric acid; somatic embryogenesis; reporter genes; genetic variation; plant morphology; beta-glucuronidase; India
Abstract:
... By manipulating hormone levels, light intensities and temperature, we have developed an efficient leaf-disc method for the regeneration of plants via embryogenesis and for transformation in four genotypes of Vitis viniferaL. In MS basal medium supplemented with 1 mg l–¹6-benzylaminopurine (BAP) and 0.1 mg l–¹2,4-dichlorophenoxyacetic acid, leaf discs cultured for 2 weeks under dark conditions prod ...
DOI:
10.1007/s00299-002-0441-4

32. Agrobacterium mediated transformation of chickpea (Cicer arietinum L.) embryo axes

Author:
Krishnamurthy, K.V.; Suhasini, K.; Sagare, A.P.; Meixner, M.; De Kathen, A.; Pickardt, T.; Schieder, O.
Source:
Plant cell reports 2000 v.19 no.3 pp. 235-240
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; glufosinate; micropropagation; neomycin; Cicer arietinum; cell biology; shoots; in vitro culture; dose response; Agrobacterium radiobacter; clones; gene expression; cultivars; phosphotransferases (phosphomutases); enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; embryo (plant); genetic variation; plant morphology; beta-glucuronidase
Abstract:
... Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 ...
DOI:
10.1007/s002990050005

33. Agrobacterium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Author:
Zhang, F.L.; Takahata, Y.; Watanabe, M.; Xu, J.B.
Source:
Plant cell reports 2000 v.19 no.6 pp. 569-575
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; cultivars; Brassica rapa subsp. pekinensis; kanamycin; dose response; transgenic plants; Agrobacterium radiobacter; explants; cotyledons; gene expression; benzyladenine; reporter genes; naphthaleneacetic acid; genetic variation; enzyme activity; culture media; beta-glucuronidase
Abstract:
... A procedure for producing transgenic Chinese cabbage plants by inoculating cotyledonary explants with Agrobacterium tumefaciens strain EHA101 carrying a binary vector pIG121Hm, which contains kanamycin-resistance and hygromycin-resistance genes and the GUS reporter gene, is described. Infection was most effective (highest infection frequency) when explants were infected with Agrobacterium for 15 m ...
DOI:
10.1007/s002990050775

34. Agrobacterium -mediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (AAB)

Author:
T. R. Ganapathi; N. S. Higgs; P. J. Balint-Kurti; C. J. Arntzen; G. D. May; J. M. Van Eck
Source:
Plant cell reports 2001 v.20 no.2 pp. 157-162
ISSN:
0721-7714
Subject:
developmental stages; genes; benzyladenine; 2,4-D; dose response; Agrobacterium radiobacter; gibberellic acid; gene expression; cultivars; beta-glucuronidase; histology; enzyme activity; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; Musa acuminata; genetic variation; plant morphology; cell suspension culture; India
Abstract:
... A protocol was developed for establishing embryogenic suspension cultures from in vitro-grown, thin shoot-tip sections of the banana cultivar Rasthali. The best medium for callus induction was an MS-based medium supplemented with 2 mg/l 2,4-D and 0.2 mg/l zeatin. The callus was transferred to liquid medium to establish embryogenic cell suspensions. These cultures were subsequently used for Agrobac ...
DOI:
10.1007/s002990000287

35. Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli

Author:
Montoro, P.; Teinseree, N.; Rattana, W.; Kongsawadworakul, P.; Michaux-Ferriere, N.
Source:
Plant cell reports 2000 v.19 no.9 pp. 851-855
ISSN:
0721-7714
Subject:
Hevea brasiliensis; genetic transformation; gene transfer; in vitro culture; dose response; transgenic plants; Agrobacterium radiobacter; gene expression; benzyladenine; micropropagation; abscisic acid; culture media; beta-glucuronidase; callus
Abstract:
... The influence of CaCl(2) was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl(2). Five A. tumefaciens strains C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the strain EHA105pC2301 was selected to conduct ...
DOI:
10.1007/s002990000208

36. Efficient organogenesis of an Australian passionfruit hybrid (Passiflora edulis x Passiflora edulis var. flavicarpa) suitable for gene delivery

Author:
Hall, R.M.; Drew, R.A.; Higgins, C.M.; Dietzgen, R.G.
Source:
Australian journal of botany 2000 v.48 no.5 pp. 673-680
ISSN:
0067-1924
Subject:
benzyladenine; genes; micropropagation; shoots; organogenesis; hybrids; dose response; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; coconut milk; enzyme activity; histochemistry; culture media; gene transfer; transgenic plants; cotyledons; beta-glucuronidase; Passiflora edulis; Queensland
DOI:
10.1071/BT99067

37. High-frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures

Author:
Rajasekaran, K.; Hudspeth, R.L.; Cary, J.W.; Anderson, D.M.; Cleveland, T.E.
Source:
Plant cell reports 2000 v.19 no.6 pp. 539-545
ISSN:
0721-7714
Subject:
Gossypium hirsutum; cell suspension culture; genetic transformation; gene transfer; transgenic plants; gene expression; beta-glucuronidase; enzyme activity; embryogenesis; culture media; dose response; developmental stages; plant anatomy; histochemistry; naphthaleneacetic acid
Abstract:
... Stable transformation of cotton (Gossypium hirsutum L.) at a high frequency has been obtained by particle bombardment of embryogenic cell suspension cultures. Transient and stable expression of the beta-glucuronidase (GUS) gene was monitored in cell suspension cultures. Transient expression, measured 48 h after bombardment, was abundant, and stable expression was observed in over 4% of the transie ...
Handle:
10113/23551
DOI:
10.1007/s002990050770

38. Production of transgenic gentian plants by particle bombardment of suspension-culture cells

Author:
Hosokawa, K.; Matsuki, R.; Oikawa, Y.; Yamamura, S.
Source:
Plant cell reports 2000 v.19 no.5 pp. 454-458
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; dose response; beta-glucuronidase; transgenic plants; enzyme activity; culture media; gene expression; phosphotransferases (phosphomutases); genes; naphthaleneacetic acid; Gentiana; histochemistry; cell suspension culture
DOI:
10.1007/s002990050755

39. Transformation of floral organs with GFP in Medicago truncatula

Author:
Kamate, K.; Rodriguez-Llorente, I.D.; Scholte, M.; Durand, P.; Ratet, P.; Kondorosi, E.; Kondorosi, A.; Trinh, T.H.
Source:
Plant cell reports 2000 v.19 no.7 pp. 647-653
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; in vitro culture; dose response; transgenic plants; enzyme activity; developmental stages; indole butyric acid; flowers; gene expression; plant morphology; benzyladenine; histochemistry; reporter genes; Medicago truncatula; 2,4-D; culture media; beta-glucuronidase
DOI:
10.1007/s002999900168

40. Agrobacterium-mediated genetic transformation of California poppy, Eschscholzia californica Cham., via somatic embryogenesis

Author:
Park, S.U.; Facchini, P.J.
Source:
Plant cell reports 2000 v.19 no.10 pp. 1006-1012
ISSN:
0721-7714
Subject:
isoquinoline alkaloids; developmental stages; genes; micropropagation; neomycin; dose response; Agrobacterium radiobacter; gene expression; naphthaleneacetic acid; phosphotransferases (phosphomutases); Eschscholzia californica; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; biosynthesis; transgenic plants; cotyledons; somatic embryogenesis; beta-glucuronidase
Abstract:
... An efficient Agrobacterium-mediated protocol for the stable genetic transformation of Eschscholzia californica Cham. (California poppy) via somatic embryogenesis is reported. Excised cotyledons were co-cultivated with A. tumefaciens strain GV3101 carrying the pBI121 binary vector. Except for the co-cultivation medium, all formulations included 50 mg l-1 paromomycin as the selective agent and 200 m ...
DOI:
10.1007/s002990000213

41. Agrobacterium-mediated genetic transformation of a phalaenopsis orchid

Author:
Belarmino, M.M.; Mii, M.
Source:
Plant cell reports 2000 v.19 no.5 pp. 435-442
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; hybrids; in vitro culture; abscisic acid; dose response; plant anatomy; transgenic plants; Agrobacterium radiobacter; developmental stages; gene expression; histochemistry; benzyladenine; naphthaleneacetic acid; Phalaenopsis; enzyme activity; culture media; beta-glucuronidase
DOI:
10.1007/s002990050752

42. Delayed recovery of beta-glucuronidase activity driven by an Arabidopsis heat shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia

Author:
Moriwaki, M.; Yamakawa, T.; Washino, T.; Kodama, T.; Igarashi, Y.
Source:
Plant cell reports 1999 v.19 no.1 pp. 96-100
ISSN:
0721-7714
Subject:
heat shock proteins; genetic transformation; Arabidopsis thaliana; gene transfer; promoter regions; in vitro culture; dose response; transgenic plants; plant growth substances; stress response; Nicotiana plumbaginifolia; gene expression; genes; micropropagation; temperature; enzyme activity; culture media; beta-glucuronidase
Abstract:
... The expression of the Arabidopsis heat shock protein (HSP) 18.2 promoter-beta-D-glucuronidase (GUS) chimera gene was investigated in transgenic Nicotiana plumbaginifolia plants during the recovery phase at normal temperatures (20-22 degrees C) after a heat shock (HS) treatment. GUS activity increased during the recovery phase after HS at 42 degrees C for 2 h, and maximal GUS activity was observed ...
DOI:
10.1007/s002990050717

43. Factors affecting Agrobacterium tumefaciens-mediated transformation of peppermint

Author:
Niu, X.; Li, X.; Veronese, P.; Bressan, R.A.; Weller, S.C.; Hasegawa, P.M.
Source:
Plant cell reports 2000 v.19 no.3 pp. 304-310
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; dose response; transgenic plants; Agrobacterium radiobacter; enzyme activity; developmental stages; explants; gene expression; histochemistry; genes; leaves; Mentha piperita; culture media; beta-glucuronidase; genetic markers
Abstract:
... Substantial improvement in peppermint (Mentha x piperita L. Black Mitcham) genetic transformation has been achieved so that the frequency of transgenic plants regenerated (percent of leaf explants that produced transformed plants) was 20-fold greater than with the original protocol. Essential modifications were made to conditions for Agrobacterium tumefaciens co-cultivation that enhanced infection ...
DOI:
10.1007/s002990050017

44. Genetic transformation of Cymbidium orchid by particle bombardment

Author:
Yang, J.; Lee, H.J.; Shin, D.H.; Oh, S.K.; Seon, J.H.; Paek, K.Y.; Han, K.H.
Source:
Plant cell reports 1999 v.18 no.12 pp. 978-984
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; in vitro culture; dose response; cell growth; transgenic plants; gene expression; genes; Cymbidium; benzyladenine; naphthaleneacetic acid; enzyme activity; culture media; beta-glucuronidase; shoots
Abstract:
... A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH20 ...
DOI:
10.1007/s002990050694

45. Agrobacterium tumefaciens-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants

Author:
Ho, C.K.; Chang, S.H.; Tsay, J.Y.; Tsai, C.J.; Chiang, V.L.; Chen, Z.Z.
Source:
Plant cell reports 1998 v.17 no.9 pp. 675-680
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; neomycin; 2,4-D; roots; callus; organogenesis; shoots; in vitro culture; dose response; Agrobacterium radiobacter; gene expression; naphthaleneacetic acid; phosphotransferases (phosphomutases); enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; Eucalyptus camaldulensis; transgenic plants; indole butyric acid; plant morphology; beta-glucuronidase
Abstract:
... An efficient system for Agrobacterium-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants was developed. Transformation was accomplished by cocultivation of hypocotyl segments with Agrobacterium tumefaciens containing a binary Ti-plasmid vector harboring chimeric neomycin phosphotransferase and beta-glucuronidase (GUS) genes. A modified Gamborg's B5 medium used ...
DOI:
10.1007/s002990050464

46. Comparison of different methods for plant regeneration and transformation of the legume Galega orientalis Lam. (goat's rue)

Author:
Collen, A.M.C.; Jarl, C.I.
Source:
Plant cell reports 1999 v.19 no.1 pp. 13-19
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; genes; micropropagation; 2,4-D; Agrobacterium; Galega orientalis; callus; shoots; dose response; meristems; explants; gene expression; naphthaleneacetic acid; enzyme activity; histochemistry; culture media; indole acetic acid; electroporation; genetic transformation; gene transfer; beta-glucuronidase
Abstract:
... For the first time, regeneration and transformation have been achieved from the legume Galega orientalis Lam.(goat's rue). Two different regeneration protocols are described, one based on direct shoot induction from meristems and the other involving callus induction and shoot induction from callus with the plant growth regulator thidiazuron (TDZ). Different media and explants were evaluated. Three ...
DOI:
10.1007/s002990050703

47. Organ-specific expression of beta-glucuronidase activity driven by the Arabidopsis heat-shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia

Author:
Moriwaki, M.; Yamakawa, T.; Washino, T.; Kodama, T.; Igarashi, Y.
Source:
Plant cell reports 1999 v.19 no.1 pp. 92-95
ISSN:
0721-7714
Subject:
genes; micropropagation; Nicotiana plumbaginifolia; promoter regions; in vitro culture; dose response; gene expression; leaves; Arabidopsis thaliana; temperature; enzyme activity; culture media; heat shock proteins; genetic transformation; gene transfer; transgenic plants; plant growth substances; stress response; flowers; beta-glucuronidase
Abstract:
... The expression of the Arabidopsis heat-shock protein (HSP) 18.2 promoter beta-D-glucuronidase (GUS) chimera gene was studied in various organs of the transgenic Nicotiana plumbaginifolia during the recovery phase at normal temperatures (20-22 degrees C) following heat-shock (HS) treatment. The optimum HS temperature for GUS activity in the anthers, petals and capsules was 42 degrees C, but in imma ...
DOI:
10.1007/s002990050716

48. Regeneration of transgenic plants of Mexican lime from Agrobacterium rhizogenes-transformed tissues

Author:
Perez-Molphe-Balch, E.; Ochoa-Alejo, N.
Source:
Plant cell reports 1998 v.17 no.8 pp. 591-596
ISSN:
0721-7714
Subject:
developmental stages; Rhizobium rhizogenes; genes; benzyladenine; micropropagation; roots; shoots; dose response; explants; gene expression; naphthaleneacetic acid; cefotaxime; Citrus aurantiifolia; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; kanamycin; transgenic plants; plant morphology; beta-glucuronidase
Abstract:
... Transgenic Mexican lime [Citrus aurantifolia (Christm.) Swing] plants were regenerated from tissues transformed by Agrobacterium rhizogenes strain A4, containing the wild-type plasmid pRiA4 and the binary vector pESC4 with nos-npt II and cab-gus genes. Transgenic shoots were generated by two different approaches. The first approach used internodal stem segments cocultured with A. rhizogenes. These ...
DOI:
10.1007/s002990050448

49. Transformation of arctic bramble (Rubus arcticus L.) by Agrobacterium tumefaciens

Author:
Kokko, H.I.; Karenlampi, S.O.
Source:
Plant cell reports 1998 v.17 no.10 pp. 822-826
ISSN:
0721-7714
Subject:
plasmids; benzyladenine; micropropagation; in vitro culture; dose response; stems; Agrobacterium radiobacter; gene expression; isopentenyladenine; naphthaleneacetic acid; kinetin; enzyme activity; culture media; indole acetic acid; genetic transformation; gene transfer; transgenic plants; indole butyric acid; Rubus arcticus; genetic vectors; beta-glucuronidase
Abstract:
... Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA 101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which beta-glucuronidase (GUS) is expressed only in plants. Regenerants were produced on Murashige and Skoog mediu ...
DOI:
10.1007/s002990050491

50. Transgenic almond (Prunus dulcis Mill.) plants obtained by Agrobacterium-mediated transformation of leaf explants

Author:
Miguel, C.M.; Oliveira, M.M.
Source:
Plant cell reports 1999 v.18 no.5 pp. 387-393
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; micropropagation; neomycin; genetic markers; dose response; Agrobacterium radiobacter; explants; gene expression; leaves; phosphotransferases (phosphomutases); enzyme activity; culture media; Prunus dulcis; indole acetic acid; genetic transformation; gene transfer; transgenic plants; indole butyric acid; plant morphology; beta-glucuronidase
Abstract:
... Almond (Prunus dulcis Mill.) leaves were transformed with the marker genes gusA (beta-glucuronidase) and nptII (neomycin phosphotransferase II) via Agrobacterium-mediated transformation. Bacterial strains and pre-culture of explants affected efficiency of gene transfer evaluated by transient expression assays. Following transformation, shoots were induced from primary explants on medium without ka ...
DOI:
10.1007/s002990050591