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You searched for: Subject term enterohemorrhagic Escherichia coli Remove constraint Subject term: enterohemorrhagic Escherichia coli Subject term foods Remove constraint Subject term: foods
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1. Glycomacropeptide Reduces Intestinal Epithelial Cell Barrier Dysfunction and Adhesion of Entero-Hemorrhagic and Entero-Pathogenic Escherichia coli in Vitro

Author:
Shane Feeney; Joseph Thomas Ryan; Michelle Kilcoyne; Lokesh Joshi; Rita Hickey
Source:
Foods 2017 v.6 no.11 pp. -
ISSN:
2304-8158
Subject:
adhesion; cheesemaking; chymosin; electrical resistance; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; foods; gastroenteritis; glycoconjugates; glycopeptides; glycosylation; human cell lines; ingredients; intestinal mucosa; kappa-casein; milk; pathogens; prebiotics; whey
Abstract:
... In recent years, the potential of glycosylated food components to positively influence health has received considerable attention. Milk is a rich source of biologically active glycoconjugates which are associated with antimicrobial, immunomodulatory, anti-adhesion, anti-inflammatory and prebiotic properties. Glycomacropeptide (GMP) is the C-terminal portion of kappa-casein that is released from wh ...
DOI:
10.3390/foods6110093
PubMed:
29077065
PubMed Central:
PMC5704137

2. A scientific and interdisciplinary approach for high pressure processing as a future toolbox for safe and high quality products: A review

Author:
R. Sevenich; C. Rauh; D. Knorr
Source:
Innovative food science & emerging technologies 2016 v.38 pp. 65-75
ISSN:
1466-8564
Subject:
bacterial spores; cold; enterohemorrhagic Escherichia coli; food matrix; foods; high pressure treatment; industry; ingredients; lipids; microorganisms; oils; pasteurization; solutes; synergism; temperature; water activity
Abstract:
... High pressure (HP) processing, typically at 600MPa in combination with mild temperatures, makes it possible to produce safe and high quality foods while inactivating pathogenic microorganisms such as Enterohaemorrhagic Escherichia coli (EHEC). In the last decade much research was conducted to understand the inactivation of spores at high pressures and elevated temperatures. Bacterial spore inactiv ...
DOI:
10.1016/j.ifset.2016.09.013

3. Enterohemorrhagic Escherichia coli (EHEC) in water from karst springs: detection with real-time PCR and isolation of strains

Author:
Andreas Baumgartner; Isabel Niederhauser; David Diston; Dominik Moor
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit 2016 v.11 no.4 pp. 353-357
ISSN:
1661-5751
Subject:
Shiga-like toxins; enterohemorrhagic Escherichia coli; food safety; foods; genes; intimin; karsts; quantitative polymerase chain reaction; springs (water); virulence; virulent strains; Switzerland
Abstract:
... Within 2 months, two water sources in a karst area in Switzerland were sampled 9 times each, and analyzed by real-time PCR for 6 EHEC O-types, Shiga-like-toxin (stx1 and stx2) and intimin (eae) genes. With the exception of O111, 5 O-types were recorded regularly and at high frequencies (O26: 33.3 %; O157: 33.3 %; O104: 66.6 %; O103: 72.2 %; O145: 94.4 %). Genes for Shiga-like-toxins and intimin we ...
DOI:
10.1007/s00003-016-1053-1

4. Enterohemorrhagic Escherichia coli Colony Check Assay for the Identification of Serogroups O26, O45, O103, O111, O121, O145, and O157 Colonies Isolated on Plating Media

Author:
Burton Blais; Mylene Deschenes; George Huszczynski; Martine Gauthier
Source:
Journal of food protection 2014 v.77 no.7 pp. 1212-1218
ISSN:
0362-028X
Subject:
antibodies; antigens; culture media; enterohemorrhagic Escherichia coli; foods; goats; lipopolysaccharides; polyesters; screening; serotypes
Abstract:
... A simple immunoenzymatic enterohemorrhagic Escherichia coli (EHEC) colony check (ECC) assay was developed for the presumptive identification of priority EHEC colonies isolated on plating media from enrichment broth cultures of foods. With this approach, lipopolysaccharide extracted from a colony is spotted on the grid of a polymyxin-coated polyester cloth strip, and bound E. coli serogroup O26, O4 ...
DOI:
10.4315/0362-028X.JFP-13-555

5. A Simple PCR-Based Macroarray System for Detection of Multiple Gene Markers in the Identification of Priority Enterohemorrhagic Escherichia coli

Author:
Burton W. Blais; Amalia Martinez-Perez
Source:
Journal of food protection 2011 v.74 no.3 pp. 365-372
ISSN:
0362-028X
Subject:
bacteria; digoxigenin; enrichment culture; enterohemorrhagic Escherichia coli; epitopes; food inspection; foodborne illness; foods; genes; hybridization; oligonucleotide probes; polyesters; polymerase chain reaction; virulence
Abstract:
... Enterohemorrhagic Escherichia coli (EHEC) strains bearing the O antigenic determinants O157, O26, O111, O103, and O145 have a high rate of association with foodborne illness worldwide. To expand Canadian food inspection capability, a cloth-based hybridization array system (CHAS) was developed for the identification and characterization of priority EHEC. This method targets key virulence genes (eae ...
DOI:
10.4315/0362-028X.JFP-10-450

6. Antimicrobial effects of weak acids on the survival of Escherichia coli O157:H7 under anaerobic conditions

Author:
Lu, Huiying J.; Breidt, Frederick Jr.; Perez-Diaz, Ilenys M.; Osborne, Jason A.
Source:
Journal of food protection 2011 v.74 no.6 pp. 893-898
Subject:
acidity; foods; food packaging; malic acid; fruit products; lactic acid; mechanism of action; acid tolerance; bacterial contamination; benzoic acid; dissolved oxygen; anaerobic conditions; sorbic acid; food preservatives; antibacterial properties; vegetable products; viability; acetic acid; sulfite liquor; food pathogens; pH; Escherichia coli O157; fumaric acid
Abstract:
... Outbreaks of disease due to vegetative bacterial pathogens associated with acid foods (such as apple cider) have raised concerns about acidified vegetables and related products that have a similar pH (3.2 to 4.0). Escherichia coli O157:H7 and related strains of enterohemorrhagic E. coli (EHEC) have been identified as the most acid resistant vegetative pathogens in these products. Previous research ...
Handle:
10113/49548
DOI:
10.4315/0362-028X.JFP-10-404

7. Low-Density Macroarray Targeting Non-Locus of Enterocyte Effacement Effectors (nle Genes) and Major Virulence Factors of Shiga Toxin-Producing Escherichia coli (STEC): a New Approach for Molecular Risk Assessment of STEC Isolates

Author:
Bugarel, Marie; Beutin, Lothar; Fach, Patrick
Source:
Applied and environmental microbiology 2010 v.76 no.1 pp. 203-211
ISSN:
0099-2240
Subject:
foods; genomic islands; humans; public health; serotypes; niches; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; risk assessment; children; genes; genotyping; host strains; virulence
Abstract:
... Rapid and specific detection of Shiga toxin-producing Escherichia coli (STEC) strains with a high level of virulence for humans has become a priority for public health authorities. This study reports on the development of a low-density macroarray for simultaneously testing the genes stx₁, stx₂, eae, and ehxA and six different nle genes issued from genomic islands OI-122 (ent, nleB, and nleE) and O ...
DOI:
10.1128/AEM.01921-09
PubMed:
19880649
PubMed Central:
PMC2798666

8. PCR Detection of Enterohemorrhagic Escherichia coli O145 in Food by Targeting Genes in the E. coli O145 O-Antigen Gene Cluster and the Shiga Toxin 1 and Shiga Toxin 2 Genes

Author:
Fratamico, Pina M.; DebRoy, Chitrita; Miyamoto, Takahisa; Liu, Yanhong
Source:
Foodborne pathogens & disease 2009 v.6 no.5 pp. 605-611
ISSN:
1535-3141
Subject:
Shiga-like toxin 1; foods; bacterial contamination; polymerase chain reaction; rapid methods; hemolytic uremic syndrome; genes; microbial detection; Shiga-like toxin 2; food pathogens; Escherichia coli O157; molecular systematics; nucleotide sequences; virulence
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) strains belonging to serogroup O145 are an important cause of hemorrhagic colitis and hemolytic uremic syndrome worldwide. Cattle and other animals are potential reservoirs for this pathogen. To develop PCR assays for detection and identification of E. coli O145, the wzx (O-antigen flippase) and wzy (O-antigen polymerase) genes in the O145 O-antigen ge ...
Handle:
10113/31931
DOI:
10.1089/fpd.2008.0254
PubMed:
19435408

9. Evaluation of Major Types of Shiga Toxin 2e-Producing Escherichia coli Bacteria Present in Food, Pigs, and the Environment as Potential Pathogens for Humans

Author:
Beutin, Lothar; Krüger, Ulrike; Krause, Gladys; Miko, Angelika; Martin, Annett; Strauch, Eckhard
Source:
Applied and environmental microbiology 2008 v.74 no.15 pp. 4806-4816
ISSN:
0099-2240
Subject:
Shiga toxin; bacteriophages; diarrhea; enterohemorrhagic Escherichia coli; enterotoxins; enzyme-linked immunosorbent assay; foods; genes; heat stability; hemolytic uremic syndrome; humans; intimin; mitomycin; nucleotide sequences; pathogens; polymerase chain reaction; sequence analysis; serotypes; slaughter; swine; virulence
Abstract:
... Shiga toxin 2e (Stx2e)-producing strains from food (n = 36), slaughtered pigs (n = 25), the environment (n = 21), diseased pigs (n = 19), and humans (n = 9) were investigated for production of Stx2e by enzyme-linked immunosorbent assay, for virulence markers by PCR, and for their serotypes to evaluate their role as potential human pathogens. Stx2e production was low in 64% of all 110 strains. Stx2 ...
DOI:
10.1128/AEM.00623-08
PubMed:
18515483
PubMed Central:
PMC2519352