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You searched for: Subject term equipment Remove constraint Subject term: equipment Subject term genetically modified organisms Remove constraint Subject term: genetically modified organisms Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. 070 Production of donor-derived offspring by allogenic transplantation of cryopreserved spermatogonia in medaka

Author:
Shinsuke Seki; Seungki Lee; Yoshiko Iwasaki; Masaru Yagisawa; Tadashi Hiratsuka; Kazunari Kusano; Misako Miwa; Sumie Endo; Mariko Ishida; Takao Sasado; Kiyoshi Naruse; Goro Yoshizaki
Source:
Cryobiology 2013 v.67 no.3 pp. 417
ISSN:
0011-2240
Subject:
accidents; allografting; breeding stock; color; cryopreservation; cryoprotectants; equipment; females; ficoll; fish; fish eggs; fluorescence; genetically modified organisms; glycerol; inbred lines; infectious diseases; larvae; males; mutation; oocytes; progeny; propylene glycol; rearing; risk; spermatogonia; spermatozoa; sucrose; survival rate; testes; toxicity; triploidy; vitrification
Abstract:
... Although numerous inbred lines and endangered wild populations of medaka exist, the only method currently available for preserving the resources is to rear live individuals, as protocols for cryopreservation of fish eggs or embryos have not yet been successfully established due to their large size. However, rearing live individuals is always associated with the risk of mutation due to mobile eleme ...
DOI:
10.1016/j.cryobiol.2013.09.076

2. Agrobacterium rhizogenes transformed soybeans with AtPAP18 gene show enhanced phosphorus uptake and biomass production

Author:
Mehdi Younessi-Hamzekhanlu; Ali Izadi-Darbandi; Mohammad Ali Malboobi; Mohsen Ebrahimi; Moslem Abdipour; Francesca Sparvoli; Dario Paolo
Source:
Biotechnology, biotechnological equipment 2018 v.32 no.4 pp. 865-873
ISSN:
1314-3530
Subject:
Agrobacterium; Arabidopsis thaliana; Rhizobium rhizogenes; biomass production; biotechnology; crop yield; enzymes; equipment; fertilizers; fluorescence; genetically modified organisms; inorganic phosphorus; phosphates; plant development; reporter genes; reverse transcriptase polymerase chain reaction; soil; soybeans
Abstract:
... Low-phosphorus stress is a challenging factor in limiting plant development. Soybean is cultivated in soils often low in phosphorus. However, on average 65% of total P is in the form of organic phosphates, which are unavailable to plants unless hydrolyzed to release inorganic phosphate. One approach for enhancing crop P acquisition from organic P sources is boosting the activity of acid phosphatas ...
DOI:
10.1080/13102818.2018.1473053

3. Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum L. cv. Hezuo 908) with improved efficiency

Author:
Sheng Sun; Xiu-Ping Kang; Xiao-Juan Xing; Xiao-Yong Xu; Jiao Cheng; Shao-Wen Zheng; Guo-Ming Xing
Source:
Biotechnology, biotechnological equipment 2015 v.29 no.5 pp. 861-868
ISSN:
1314-3530
Subject:
Northern blotting; Solanum lycopersicum var. lycopersicum; absorbance; benzyladenine; biotechnology; coculture; cotyledons; equipment; genetic transformation; genetically modified organisms; hypocotyls; indole acetic acid; liquids; quantitative polymerase chain reaction; rooting; tomatoes
Abstract:
... We developed an efficient and rapid Agrobacterium-mediated tomato transformation protocol by using cotyledon and hypocotyl as explants. The transgenic nature of the regenerants was confirmed through β-glucuronidase activity staining, polymerase chain reaction (PCR), quantitative real-time PCR (qPCR) analysis and northern blot analysis. In the used protocol, the optimized experimental conditions of ...
DOI:
10.1080/13102818.2015.1056753

4. Anthocyanin, a novel and user-friendly reporter for convenient, non-destructive, low cost, directly visual selection of transgenic hairy roots in the study of rhizobia-legume symbiosis

Author:
Yinglun Fan; Xiuyuan Wang; Haiyun Li; Shuang Liu; Liangshen Jin; Yanyan Lyu; Mengdi Shi; Sirui Liu; Xinyue Yang; Shanhua Lyu
Source:
Plant methods 2020 v.16 no.1 pp. 94
ISSN:
1746-4811
Subject:
Agrobacterium; Arabidopsis; Glycine max; Lotus; Medicago truncatula; Sinorhizobium fredii; anthocyanins; color; crops; equipment; eyes; genetically modified organisms; models; mutagenesis; nitrogen fixation; nodulation; reliability; reporter genes; roots; soybeans; symbiosis; transcription factors
Abstract:
... BACKGROUND: Agrobacterium rhizogenes-mediated hairy root transformation provides a powerful tool for investigating the functions of plant genes involved in rhizobia-legume symbiosis. However, in the traditional identification methods of transgenic hairy roots based on reporter genes, an expensive chemical substrate or equipment is required. RESULTS: Here, we report a novel, low cost, and robust re ...
DOI:
10.1186/s13007-020-00638-w

5. Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli

Author:
Kai P. Yuet; Chaitan Khosla
Source:
Metabolic Engineering Communications 2020 v.10 pp. e00106
ISSN:
2214-0301
Subject:
Escherichia coli; biosynthesis; drugs; engineering; equipment; genetically modified organisms; polyketide synthases; polyketides
Abstract:
... Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical strategy for understanding them. Here, we concisely summarize successful examples in exploiting E. coli for assembly-line polyketide biosynthesis as wel ...
DOI:
10.1016/j.mec.2019.e00106

6. Collaborative trial to assess the performance of digital PCR in the field of GMO analysis using an artificial sample material

Author:
René Köppel; Verena Peterseil; Emilie Dagand; Ekkehard Schütz; Nina Kolberg; Mojca Milavec; Dominik Moor
Source:
European food research & technology 2017 v.243 no.6 pp. 1091-1096
ISSN:
1438-2377
Subject:
DNA; chemical elements; equipment; genetically modified organisms; lectins; polymerase chain reaction; transgenes
Abstract:
... Digital PCR promises absolute quantification of amplifiable nucleic acids molecules. In consequence, results should be comparable, irrespective of the analysing laboratory and the equipment used. To assess this claim, we used a multitarget DNA molecule (amplicon) encoding for eight transgene soy traits and the lectin soy reference gene. This multitarget amplicon was analysed by seven European labo ...
DOI:
10.1007/s00217-016-2824-8

7. Efficient agrobacterium-mediated transformation of Shiraia bambusicola and activation of a specific transcription factor for hypocrellin production

Author:
Tong Li; Cheng-Lin Hou; Xiao-Ye Shen
Source:
Biotechnology, biotechnological equipment 2019 v.33 no.1 pp. 1365-1371
ISSN:
1314-3530
Subject:
Agrobacterium radiobacter; bamboos; biosynthesis; biotechnology; equipment; fungi; genes; genetic transformation; genetically modified organisms; mycelium; quantitative polymerase chain reaction; transcription factors
Abstract:
... Shiraia bambusicola, as a well-known medicinal and edible macro-fungus, is generally located on the branches of bamboo and plays an important role in herbal treatment. Furthermore, the natural products from its fruiting bodies, hypocrellins, are attracting increasing amounts of attention due to their notable bioactivities. In this report, we focused on constructing a fast and universal transgenic ...
DOI:
10.1080/13102818.2019.1667874

8. Efficient production of D-HPG with an immobilized transgenic strain E. coli LY13-05

Author:
Xiaobing Hu; Biaosheng Lin
Source:
Biotechnology, biotechnological equipment 2015 v.29 no.5 pp. 1003-1010
ISSN:
1314-3530
Subject:
Escherichia coli; application timing; biotechnology; equipment; fermentation; genetically modified organisms; lactose; storage time; thermal stability
Abstract:
... The aim of the present study was to construct a recombinant strain of Escherichia coli LY13-05 that simultaneously expresses D-hydantoinase (Dhase) and D-decarbamoylase (Dcase) and exhibits a high-efficiency substrate conversion with a specific yield rate, close to those reported in the literature for other bacteria. The cultivation conditions, fermentation properties and immobilized recombinant s ...
DOI:
10.1080/13102818.2015.1044909

9. Evaluation of quantitative PCR methods for genetically modified maize (MON863, NK603, TC1507 and T25)

Author:
Takabatake, Reona; Futo, Satoshi; Minegishi, Yasutaka; Watai, Masatoshi; Sawada, Chihiro; Nakamura, Kosuke; Akiyama, Hiroshi; Teshima, Reiko; Furui, Satoshi; Hino, Akihiro; Kitta, Kazumi
Source:
Food science and technology research 2010 v.16 no.5 pp. 421-430
ISSN:
1344-6606
Subject:
corn; equipment; genetically modified organisms; quantitative analysis; quantitative polymerase chain reaction
Abstract:
... Novel real-time PCR-based quantitative methods were developed for three GM maize events; MON863, NK603 and TC1507. The quantitative methods were designed to amplify an event-specific segment for MON863 and NK603, and a construct-specific segment for TC1507. We also developed an event-specific quantitative method for T25. The conversion factor (Cf), which is required for calculating the GMO amount, ...
DOI:
10.3136/fstr.16.421

10. Evaluation of the role of Medicago truncatula Zn finger CCHC type protein after heterologous expression in Arabidopsis thaliana

Author:
Mariana Radkova; Miglena Revalska; Miroslava Zhiponova; Anelia Iantcheva
Source:
Biotechnology & Biotechnological Equipment 2021 v.35 no.1 pp. 1686-1695
ISSN:
1314-3530
Subject:
Arabidopsis thaliana; Medicago truncatula; apical meristems; biotechnology; cold; cold stress; cold treatment; computer simulation; equipment; gain-of-function mutation; genes; genetic markers; genetically modified organisms; heterologous gene expression; plant reproduction; seed development; seed size; shoot meristems; signal transduction; siliques; transcription (genetics); zinc finger motif
Abstract:
... Zinc finger proteins bind nucleic acids or act in transcriptional or translational regulation. The present study aimed to explore the effect of heterologous expression of the Medicago truncatula gene (Mt-Zn-CCHC), which encodes a Zinc finger CCHC type protein, in Arabidopsis thaliana. The Mt-Zn-CCHC gene, which affects seed size in M. truncatula, was used for construction of transgenic A. thaliana ...
DOI:
10.1080/13102818.2021.2006786

11. Feasibility of conventional and Roundup Ready® soybeans discrimination by different near infrared reflectance technologies

Author:
Lidia Esteve Agelet; Aoife A. Gowen; Charles R. Hurburgh Jr.; Colm P. O’Donell
Source:
Food chemistry 2012 v.134 no.2 pp. 1165-1172
ISSN:
0308-8146
Subject:
consumer demand; equipment; genetically modified organisms; neural networks; principal component analysis; reflectance; seeds; soybeans
Abstract:
... Identification and proper labelling of genetically modified organisms is required and increasingly demanded by legislation and consumers worldwide. In this study, the feasibility of three near infrared reflectance technologies (a chemical imaging unit, a commercial diode array instrument, and a light tube non-commercial instrument) were compared for discriminating Roundup Ready® and not geneticall ...
DOI:
10.1016/j.foodchem.2012.02.144
PubMed:
23107744

12. Loop-Mediated Isothermal Amplification for the Event-Specific Detection of Wheat B73-6-1

Author:
Yang Cheng; Minghui Zhang; Kun Hu; Fangda Sun; Ran Tao; Xuejun Gao; Fengxia Luan
Source:
Food analytical methods 2014 v.7 no.2 pp. 500-505
ISSN:
1936-9751
Subject:
DNA; cost effectiveness; detection limit; equipment; gel electrophoresis; genetically modified organisms; polymerase chain reaction; wheat
Abstract:
... To develop effective alternatives for detecting genetically modified organisms, we constructed one loop-mediated isothermal amplification (LAMP) event-specific detection method for wheat B73-6-1 in this study. This event-specific LAMP assay can be performed within 38 min without any PCR equipment and the LAMP products can be directly observed by the naked eye instead of conventional gel electropho ...
DOI:
10.1007/s12161-013-9718-1

13. Multiplex pyrosequencing quantitative detection combined with universal primer-multiplex-PCR for genetically modified organisms

Author:
Ying Shang; Yuancong Xu; Kunlun Huang; Yunbo Luo; Wentao Xu
Source:
Food chemistry 2020 v.320 pp. 126634
ISSN:
0308-8146
Subject:
DNA; computer software; detection limit; equipment; fluorescence; food labeling; genetically modified organisms; issues and policy; nucleotide sequences; sequence analysis
Abstract:
... A multiplex pyrosequencing quantitative detection technique combined with universal primer-multiplex-PCR (UP-M-PCR) was established. In this study, a pyrosequencing results analysis software was first self-compiled, which realized the DNA sequences degeneration, and converted the pyrosequencing results and base composition of the target sequences into mathematic relations. Five calculation models ...
DOI:
10.1016/j.foodchem.2020.126634

14. Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis

Author:
Ryan G. Bing; Christopher T. Straub; Daniel B. Sulis; Jack P. Wang; Michael W.W. Adams; Robert M. Kelly
Source:
Bioresource technology 2022 v.348 pp. 126780
ISSN:
0960-8524
Subject:
Caldicellulosiruptor bescii; Populus; acetone; biorefining; carbohydrate content; economic analysis; equipment; feedstocks; fermentation; genetically modified organisms; hydrogen; lignocellulose; metabolic engineering; models; phytomass; solubilization; soybean hulls; soybeans; thermophilic bacteria
Abstract:
... A variety of chemical and biological processes have been proposed for conversion of sustainable low-cost feedstocks into industrial products. Here, a biorefinery concept is formulated, modeled, and analyzed in which a naturally (hemi)cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii, is metabolically engineered to convert the carbohydrate content of lignocellulosic bio ...
DOI:
10.1016/j.biortech.2022.126780

15. Production of knockout mouse lines with Cas9

Author:
Marina Gertsenstein; Lauryl M.J. Nutter
Source:
Methods 2021 v.191 pp. 32-43
ISSN:
1046-2023
Subject:
electroporation; equipment; genetically modified organisms; germ cells; knockout mutants; mice; phenotype; ribonucleoproteins
Abstract:
... Knockout mice are used extensively to explore the phenotypic effects of mammalian gene dysfunction. With the application of RNA-guided Cas9 nuclease technology for the production of knockout mouse lines, the time, as well as the resources needed, to progress from identification of a gene of interest to production of a knockout line is significantly reduced. Here we present our standard methodology ...
DOI:
10.1016/j.ymeth.2021.01.005

16. Quantitative estimation of sampling uncertainties for mycotoxins in cereal shipments

Author:
F.S. Bourgeois; G.J. Lyman
Source:
Food additives & contaminants 2012 v.29 no.7 pp. 1141-1156
ISSN:
1944-0057
Subject:
Monte Carlo method; analytical methods; equipment; genetically modified organisms; heavy metals; herbicide residues; ochratoxin A; pesticides; seeds; silos; soybeans; statistical models; uncertainty; variance; wheat
Abstract:
... Many countries receive shipments of bulk cereals from primary producers. There is a volume of work that is on-going that seeks to arrive at appropriate standards for the quality of the shipments and the means to assess the shipments as they are out-loaded. Of concern are mycotoxin and heavy metal levels, pesticide and herbicide residue levels, and contamination by genetically modified organisms (G ...
DOI:
10.1080/19440049.2012.675594
PubMed:
22651820

17. Rapid analysis of poplar lignin monomer composition by a streamlined thioacidolysis procedure and near-infrared reflectance-based prediction modeling

Author:
Robinson, Andrew R.; Mansfield, Shawn D.
Source:
Plant journal 2009 v.58 no.4 pp. 706-714
ISSN:
0960-7412
Subject:
Populus alba; cell walls; equipment; genetic engineering; genetically modified organisms; germplasm screening; hybrids; least squares; lignin; models; plant breeding; prediction; rapid methods; thioacidolysis; trees; wood
Abstract:
... Determination of the physico-chemical attributes of plant cell walls, such as lignin content and composition, is of paramount importance in germplasm screening and for evaluating the results of plant breeding and genetic engineering. There are escalating needs for analyses to be robust, reproducible, accurate, and efficient. We have recently modified an established protocol for discrimination of l ...
DOI:
10.1111/j.1365-313X.2009.03808.x
PubMed:
19175772

18. Recent Developments in Molecular Detection of Food-Borne Disease Bacteria and GMOs

Author:
Robert E. Levin
Source:
Food biotechnology 2015 v.29 no.1 pp. 69-116
ISSN:
1532-4249
Subject:
DNA; Salmonella enterica; bacteria; equipment; food biotechnology; food industry; food matrix; foodborne illness; genetically modified organisms; ground beef; pathogens; polymerase chain reaction; raw foods; temperature; toxigenic strains
Abstract:
... The polymerase chain reaction (PCR) constitutes a powerful method for rapidly detecting low numbers of pathogenic and toxigenic bacteria in complex food matrices. Recent developments have resulted in the ability to detect as low as 1 CFU of Salmonella enterica in a 25g sample of ground beef within 4.5 hrs without enrichment. Isothermal DNA amplification such as loop mediated DNA amplification (LAM ...
DOI:
10.1080/08905436.2014.996896

19. Regulatory experience and challenges for the release of GM insects

Author:
Camilla Beech
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit = 2014 v.9 no.Supplement 1 pp. 71-76
ISSN:
1661-5751
Subject:
biological control; biological control agents; equipment; genetically modified organisms; human health; humans; insect control; insect pests; issues and policy; laws and regulations; risk assessment process
Abstract:
... Genetically modified (GM) insects are a potentially valuable new tool for the biological control of insect pests of humans, animals and plants. Considerable progress has been made recently in transfer of GM insects from the laboratory to release and evaluation in the environment. As with other new genetic technologies, regulatory agencies have often found it challenging to determine the regulatory ...
DOI:
10.1007/s00003-014-0886-8

20. Review of research advances in the screwworm eradication program over the past 25 years

Author:
Maxwell J. Scott; Carolina Concha; John B. Welch; Pamela L. Phillips; Steven R. Skoda
Source:
Entomologia experimentalis et applicata 2017 v.164 no.3 pp. 226-236
ISSN:
0013-8703
Subject:
Cochliomyia hominivorax; USDA; cryopreservation; equipment; females; genetic analysis; genetic variation; genetically modified organisms; habitats; ingredients; lethal genes; livestock; male sterility; mass rearing; monitoring; pests; provenance; research support; sex determination analysis; sterile insect technique; North America
Abstract:
... New World screwworms, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae: Chrysomyinae), are devastating pests of warm‐blooded animals that cause significant economic damage to livestock. The successful campaign to eradicate screwworms from continental North America, led by the US Department of Agriculture and using the sterile insect technique, continues to receive research support that h ...
DOI:
10.1111/eea.12607

21. Row-Spacing Interactions on Spring Canola Performance in the Northern Great Plains

Author:
Burton L. Johnson; Bryan K. Hanson
Source:
Agronomy journal 2003 v.95 no.3 pp. 703-708
ISSN:
0002-1962
Subject:
Brassica napus; canola; cultivars; equipment; flowering; flowers; genetically modified organisms; geography; hybrids; lipid content; lodging; open pollination; row spacing; seed oils; seed yield; sowing; Great Plains region; North Dakota
Abstract:
... Introduction of hybrid and transgenic spring canola (Brassica napus L.) cultivars and production in a new geography prompt performance evaluation of these cultivar types. The objective was to determine interactions for commonly used research and commercial production row spacings with canola performance using contemporary open-pollinated, hybrid, and transgenic cultivars. Two North Dakota rainfed ...
DOI:
10.2134/agronj2003.7030

22. Single and dual drug selection for transgenes following bombardment of Caenorhabditis species

Author:
Jennifer I. Semple; Ben Lehner
Source:
Methods 2014 v.68 pp. 409-416
ISSN:
1046-2023
Subject:
Caenorhabditis elegans; drug resistance; equipment; genetic background; genetic complementation; genetically modified organisms; mutation; puromycin; transgenes
Abstract:
... The use of drugs and drug resistance genes is a powerful method to select for the presence of a transgene. Unlike methods that require the complementation of a genetic mutation, this system can be used on any genetic background. Drug selection does not require extensive manipulation or costly equipment, yet it is very rapid and can achieve extremely high efficiency, selecting a small number of tra ...
DOI:
10.1016/j.ymeth.2014.05.004

23. Testing the Robustness of Validated Methods for Quantitative Detection of GMOs Across qPCR Instruments

Author:
E. Luque-Perez; M. Mazzara; T. P. Weber; N. Foti; E. Grazioli; B. Munaro; G. Pinski; G. Bellocchi; G. Van den Eede; C. Savini
Source:
Food analytical methods 2013 v.6 no.2 pp. 343-360
ISSN:
1936-9751
Subject:
analysis of variance; analytical methods; equipment; genetically modified organisms; polymerase chain reaction; quantitative analysis
Abstract:
... The number of real-time polymerase chain reaction (qPCR) instruments available has greatly increased over recent years. However, little information is available on the performance of validated quantitative methods when tested in different instruments. A study has been designed to evaluate the robustness of three validated methods for genetically modified organisms (GMO) quantification across six r ...
DOI:
10.1007/s12161-012-9445-z