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You searched for: Subject term extracellular matrix Remove constraint Subject term: extracellular matrix Subject term mixing Remove constraint Subject term: mixing Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A fully degradable and photocrosslinked polysaccharide-polyphosphate hydrogel for tissue engineering

Author:
Ying Hao; Jinlin He; Xun Ma; Lin Feng; Mo Zhu; Yuanxin Zhai; Yang Liu; Peihong Ni; Guosheng Cheng
Source:
Carbohydrate polymers 2019 pp. -
ISSN:
0144-8617
Subject:
alkynes; biodegradability; composite polymers; extracellular matrix; humans; hyaluronic acid; hydrogels; mesenchymal stromal cells; mixing; peptides; phosphates; tissue engineering; viscoelasticity
Abstract:
... Extracellular matrix degradability meditates cell behaviors and gains increasing importance in the development of implantation materials for tissue engineering. Here, we developed a fully biodegradable hydrogel combining the unique features of synthetic polyphosphate polymer and natural polysaccharide polymer. Polyphosphate copolymer poly(butynyl phospholane)-random-poly(ethylethylene phosphate) ( ...
DOI:
10.1016/j.carbpol.2019.115257

2. Alginate-based hydrogels with improved adhesive properties for cell encapsulation

Author:
Bapi Sarker; Julia Rompf; Raquel Silva; Nadine Lang; Rainer Detsch; Joachim Kaschta; Ben Fabry; Aldo R. Boccaccini
Source:
International journal of biological macromolecules 2015 v.78 pp. 72-78
ISSN:
0141-8130
Subject:
biocompatible materials; cell adhesion; crosslinking; extracellular matrix; gelatin; hydrocolloids; mechanical properties; microencapsulation; mixing; physicochemical properties
Abstract:
... Hydrogel-based biomaterials are ideal scaffolding matrices for microencapsulation, but they need to be modified to resemble the mechanical, structural and chemical properties of the native extracellular matrix. Here, we compare the mechanical properties and the degradation behavior of unmodified and modified alginate hydrogels in which cell adhesive functionality is conferred either by blending or ...
DOI:
10.1016/j.ijbiomac.2015.03.061

3. An injectable hydrogel formed by in situ cross-linking of glycol chitosan and multi-benzaldehyde functionalized PEG analogues for cartilage tissue engineering

Author:
Luping Cao; Bin Cao; Chengjiao Lu; Guowei Wang; Lin Yu; Jiandong Ding
Source:
Journal of materials chemistry B 2015 v.3 no.7 pp. 1268-1280
ISSN:
2050-7518
Subject:
aldehydes; cartilage; chitosan; chondrocytes; crosslinking; encapsulation; extracellular matrix; gelation; gene expression; hydrogels; in vitro culture; mechanical properties; mice; mixing; phenotype; polyethylene glycol; schiff bases; staining; tissue engineering; water uptake
Abstract:
... In this study, a multi-benzaldehyde functionalized poly(ethylene glycol) analogue, poly(ethylene oxide-co-glycidol)-CHO (poly(EO-co-Gly)-CHO), was designed and synthesized for the first time, and was applied as a cross-linker to develop an injectable hydrogel system. Simply mixing two aqueous precursor solutions of glycol chitosan (GC) and poly(EO-co-Gly)-CHO led to the formation of chemically cro ...
DOI:
10.1039/c4tb01705f

4. Assaying human neutrophil elastase activity by capillary zone electrophoresis combined with laser-induced fluorescence

Author:
Syntia Fayad; Reine Nehmé; Pierre Lafite; Philippe Morin
Source:
Journal of chromatography 2015 v.1419 pp. 116-124
ISSN:
0021-9673
Subject:
capillary zone electrophoresis; chromatography; elastase; enzyme activity; enzyme kinetics; extracellular matrix; fluorescence; hydrolysis; laminar flow; micelles; mixing; neutrophils; screening
Abstract:
... Skin aging is a progressive process determining the ultimate skin appearance. Human neutrophil elastase (HNE) has been shown to play an important role in the degradation of the extracellular matrix. In order to assay HNE kinetics, a novel online capillary zone electrophoresis (CZE) assay has been developed in this study for the determination of the maximum velocity (Vmax) and of the Michaelis–Ment ...
DOI:
10.1016/j.chroma.2015.09.084

5. Bilayered nanofibrous 3D hierarchy as skin rudiment by emulsion electrospinning for burn wound management

Author:
Pallabi Pal; Prabhash Dadhich; Pavan Kumar Srivas; Bodhisatwa Das; Dhrubajyoti Maulik; Santanu Dhara
Source:
Biomaterials science 2017 v.5 no.9 pp. 1786-1799
ISSN:
2047-4849
Subject:
Cirrhinus cirrhosus; animal models; burns (injuries); cell adhesion; coatings; collagen; cost effectiveness; emulsions; extracellular matrix; fibroblasts; keratinocytes; mixing; nanofibers; porosity; protein synthesis; scales (integument)
Abstract:
... Mimicking skin extracellular matrix hierarchy, the present work aims to develop a bilayer skin graft comprising a porous cotton-wool-like 3D layer with membranous structure of PCL–chitosan nanofibers. Emulsion electrospinning with differential stirring periods of PCL–chitosan emulsion results in development of a bilayer 3D structure with varied morphology. The electrospun membrane has fiber diamet ...
DOI:
10.1039/c7bm00174f

6. Cation−π Interaction Induced Folding of AAB-Type Collagen Heterotrimers

Author:
Chiang Chu-Harn; Horng Jia-Cherng
Source:
Journal of physical chemistry 2016 v.120 no.7 pp. 1205-1211
ISSN:
1520-5207
Subject:
circular dichroism spectroscopy; collagen; differential scanning calorimetry; extracellular matrix; mixing; nuclear magnetic resonance spectroscopy; peptides; physical chemistry
Abstract:
... Collagen is the most predominant component of the extracellular matrix. Natural collagens consist of all identical (AAA, homotrimer), two different (AAB, heterotrimer), or three different (ABC, heterotrimer) peptide chains. Many natural collagens are either AAB- or ABC-type heterotrimers, making heterotrimeric helices better mimics for studying collagen structures in nature. We prepared collagen-m ...
DOI:
10.1021/acs.jpcb.5b11189

7. Cell-loaded injectable gelatin/alginate/LAPONITE® nanocomposite hydrogel promotes bone healing in a critical-size rat calvarial defect model

Author:
Bin Liu; Junqin Li; Xing Lei; Sheng Miao; Shuaishuai Zhang; Pengzhen Cheng; Yue Song; Hao Wu; Yi Gao; Long Bi; Guoxian Pei
Source:
RSC advances 2020 v.10 no.43 pp. 25652-25661
ISSN:
2046-2069
Subject:
adverse effects; alginates; biomimetics; bone marrow; cell proliferation; encapsulation; extracellular matrix; gelatin; hydrogels; mesenchymal stromal cells; mixing; models; nanocomposites; orthopedics; rats; therapeutics; tissue engineering
Abstract:
... Injectable hydrogels have long been gaining attention in the bone tissue engineering field owing to their ability to mix homogeneously with cells and therapeutic agents, minimally invasive administration, and seamless defect filling. Despite the advantages, the use of injectable hydrogels as cell delivery carriers is currently limited by the challenge of mimicking the natural microenvironment of t ...
DOI:
10.1039/d0ra03040f

8. Characterization of biodegradable poly(lactic acid) porous scaffolds prepared using selective enzymatic degradation for tissue engineering

Author:
Ziqi Guo; Cheng Yang; Zuping Zhou; Shan Chen; Fan Li
Source:
RSC advances 2017 v.7 no.54 pp. 34063-34070
ISSN:
2046-2069
Subject:
biocompatibility; biodegradability; biomimetics; body fluids; cell culture; cell proliferation; cell viability; embryo (animal); enzymes; extracellular matrix; fibroblasts; manufacturing; mice; microstructure; mixing; polylactic acid; porosity; scanning electron microscopy; tissue engineering
Abstract:
... In this study, poly(lactic acid) (PLA) scaffolds were prepared by selective enzymatic degradation using poly(3-hydrobutyrate-co-4-hydrobutyrate) [P(3HB-co-4HB)] depolymerase. The porous morphology and properties of the scaffolds were investigated to clarify whether the microstructure of the biomimetic extracellular matrix is suitable for cell proliferation and differentiation. When the P(3HB-co-4H ...
DOI:
10.1039/c7ra03574h

9. Construction of Injectable Double-Network Hydrogels for Cell Delivery

Author:
Yan Yan; Li Mengnan; Yang Di; Wang Qian; Liang Fuxin; Qu Xiaozhong; Qiu Dong; Yang Zhenzhong
Source:
Biomacromolecules 2017 v.18 no.7 pp. 2128-2138
ISSN:
1526-4602
Subject:
calcium alginate; cartilage; cell proliferation; chemical bonding; chitosan; crosslinking; encapsulation; ethylene oxide; extracellular matrix; hydrogels; mice; mixing; polyethylene glycol
Abstract:
... Herein we present a unique method of using dynamic cross-links, which are dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and dibenzaldhyde capped poly(ethylene oxide) formed the first network, while calcium alginate formed the second one, and in the resultant ...
DOI:
10.1021/acs.biomac.7b00452

10. Degradation of endothelial network in disordered tumor-containing cell sheet

Author:
Menglu Li; Masahiro Kino-oka
Source:
Journal of bioscience and bioengineering 2017 v.123 no.6 pp. 748-753
ISSN:
1389-1723
Subject:
angiogenesis; cell movement; coculture; extracellular matrix; green fluorescent protein; human umbilical vein endothelial cells; humans; mixing; myoblasts; neoplasm cells; skeletal muscle
Abstract:
... Tumor angiogenesis is an important event in tumor malignancy; and the vasculature formed in tumor region is typically dysfunctional. Multiple factors are associated with tumor vessel abnormalities, but the precise mechanism has not been fully understood. In the present study, a tumor-containing cell sheet was prepared by mixing a small population of human embryonal rhabdomyosarcoma (RMS) cells (RD ...
DOI:
10.1016/j.jbiosc.2017.01.017

11. Different Interaction Modes of Biomolecules with Citrate-Capped Gold Nanoparticles

Author:
Zhang Shiyun; Moustafa Yasmine; Huo Qun
Source:
ACS Applied Materials & Interfaces 2014 v.6 no.23 pp. 21184-21192
ISSN:
1944-8252
Subject:
biomarkers; blood; bovine serum albumin; chemical elements; citrates; electrostatic interactions; extracellular matrix; humans; hyaluronic acid; immunoglobulin G; immunoglobulin M; ligands; light scattering; mixing; nanogold; nanoparticles; rabbits; rheumatoid arthritis; transmission electron microscopy; ultraviolet-visible spectroscopy; zeta potential
Abstract:
... In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV–vis absorption spectroscopy, and transmission electron microscopy. The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan ...
DOI:
10.1021/am506112u

12. Electrospinning of poly (ε-caprolactone-co-lactide)/Pluronic blended scaffolds for skin tissue engineering

Author:
Ning-hua Liu; Jian-feng Pan; Yue-E Miao; Tian-xi Liu; Feng Xu; Hui Sun
Source:
Journal of materials science 2014 v.49 no.20 pp. 7253-7262
ISSN:
0022-2461
Subject:
cell adhesion; contact angle; extracellular matrix; hydrophilicity; mixing; stem cells; tensile strength; tissue engineering
Abstract:
... For skin tissue engineering, an ideal scaffold should mimic the natural extracellular matrix of the native skin. In this study, we reported a novel elastic sub-micron fiber scaffold blending poly (ε-caprolactone-co-lactide) (PLCL) and Pluronic at different ratios by electrospinning. PLCL and Pluronic were co-electrospun with the ratio of 100/0, 99/1, 95/5, 90/10, 85/15, and 75/25. These scaffolds ...
DOI:
10.1007/s10853-014-8432-8

13. Extracellular Matrix-Coated Composite Scaffolds Promote Mesenchymal Stem Cell Persistence and Osteogenesis

Author:
Harvestine Jenna N.; Vollmer Nina L.; Ho Steve S.; Zikry Christopher A.; Lee Mark A.; Leach J. Kent
Source:
Biomacromolecules 2016 v.17 no.11 pp. 3524-3531
ISSN:
1526-4602
Subject:
adhesion; apoptosis; biochemical pathways; bone formation; extracellular matrix; glass; histology; humans; mixing; stem cells; tissue repair
Abstract:
... Composite scaffolds of bioactive glass and poly(lactide-co-glycolide) provide advantages over homogeneous scaffolds, yet their therapeutic potential can be improved by strategies that promote adhesion and present instructive cues to associated cells. Mesenchymal stem cell (MSC)-secreted extracellular matrix (ECM) enhances survival and function of associated cells. To synergize the benefits of an i ...
DOI:
10.1021/acs.biomac.6b01005

14. Fabrication and characterization of electrospun nanofibers composed of decellularized meniscus extracellular matrix and polycaprolactone for meniscus tissue engineering

Author:
Shuang GaoShuang Gao and Weimin Guo contributed equally to this work.; Weimin Guo; Mingxue Chen; Zhiguo Yuan; Mingjie Wang; Yu Zhang; Shuyun Liu; Tingfei Xi; Quanyi GuoCorrespondence to Institute of Orthopaedics, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.
Source:
Journal of materials chemistry B 2017 v.5 no.12 pp. 2273-2285
ISSN:
2050-7518
Subject:
Fourier transform infrared spectroscopy; centrifugation; collagen; cytotoxicity; extracellular matrix; hardness; hemolysis; humans; hydrophilicity; in vitro studies; mechanical properties; mixing; modulus of elasticity; nanofibers; reverse transcriptase polymerase chain reaction; tissue engineering
Abstract:
... Many kinds of scaffolds have been produced in meniscus tissue engineering, but few have matched the mechanical properties of native meniscus, making it impossible for them to sustain large stress at initial implantation. In this study, we used a differential centrifugation method to obtain decellularized meniscus extracellular matrix (DMECM) and combined the DMECM with polycaprolactone (PCL) via e ...
DOI:
10.1039/c6tb03299k

15. Fabrication of a bioengineered corneal endothelial cell sheet using chitosan/polycaprolactone blend membranes

Author:
Tai-Horng Young; I-Jong Wang; Fung-Rong Hu; Tsung-Jen Wang
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.116 pp. 403-410
ISSN:
0927-7765
Subject:
Western blotting; adenosinetriphosphatase; cattle; chitosan; collagen; colloids; cornea; endothelial cells; endothelium; extracellular matrix; immunohistochemistry; mixing; phenotype; polymers; potassium; sodium; staining; transmittance
Abstract:
... Our previous study has demonstrated cultivation of bovine corneal endothelial cells (BCECs) on the chitosan–polycaprolactone (PCL) blends. BCECs could grow well and express physiological phenotypes after PCL was introduced into chitosan by means of blending. The feasibility of using chitosan–PCL-blended membranes as scaffold and carrier for corneal endothelial cell (CEC) transplantation is worthy ...
DOI:
10.1016/j.colsurfb.2014.01.024

16. Fragmentation of Injectable Bioadhesive Hydrogels Affords Chemotherapeutic Macromolecules

Author:
Yamada Yuji; Schneider Joel P.
Source:
Biomacromolecules 2016 v.17 no.8 pp. 2634-2641
ISSN:
1526-4602
Subject:
aldehydes; bovine serum albumin; cell death; cytotoxins; doxorubicin; drug therapy; extracellular matrix; hydrogels; lung neoplasms; mixing; neoplasm cells
Abstract:
... Implantation of drug delivery depots into or proximal to targeted tissue is an effective method to deliver anticancer drugs in a sustained localized manner. Herein, syringe-injectable polydextran aldehyde (PDA)-based bioadhesive gels are prepared that can locally deliver cytotoxins upon their hydrolytic fragmentation. Adhesive gels are formed by mixing doxorubicin (DOX)-functionalized PDA (DOX-PDA ...
DOI:
10.1021/acs.biomac.6b00701

17. Hybrid Randomly Electrospun Poly(lactic-co-glycolic acid):Poly(ethylene oxide) (PLGA:PEO) Fibrous Scaffolds Enhancing Myoblast Differentiation and Alignment

Author:
Evrova Olivera; Hosseini Vahid; Milleret Vincent; Palazzolo Gemma; Zenobi-Wong Marcy; Sulser Tullio; Buschmann Johanna; Eberli Daniel
Source:
ACS applied materials 2016 v.8 no.46 pp. 31574-31586
ISSN:
1944-8252
Subject:
adhesion; ethylene oxide; extracellular matrix; fiber quality; hydrophilicity; mechanical properties; mixing; modulus of elasticity; myoblasts; myotubes; polyethylene glycol; spinning
Abstract:
... Cellular responses are regulated by their microenvironments, and engineered synthetic scaffolds can offer control over different microenvironment properties. This important relationship can be used as a tool to manipulate cell fate and cell responses for different biomedical applications. We show for the first time in this study how blending of poly(ethylene oxide) (PEO) to poly(lactic-co-glycolic ...
DOI:
10.1021/acsami.6b11291

18. Impact of shear force on the biofilm structure and performance of a membrane biofilm reactor for tertiary hydrogen-driven denitrification of municipal wastewater

Author:
Celmer, D.; Oleszkiewicz, J.A.; Cicek, N.
Source:
Water research 2008 v.42 no.12 pp. 3057-3065
ISSN:
0043-1354
Subject:
wastewater treatment; shear stress; biofilm; bioreactors; hydrogen; denitrification; equipment performance; mixing; nitrogen; carbohydrates; proteins; nitrates; biomass; chemical oxygen demand; carbon; artificial membranes; extracellular matrix
DOI:
10.1016/j.watres.2008.02.031
PubMed:
18396310

19. Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

Author:
Yuji Yamada; Kentaro Hozumi; Akihiro Aso; Atsushi Hotta; Kazunori Toma; Fumihiko Katagiri; Yamato Kikkawa; Motoyoshi Nomizu
Source:
Biomaterials 2012 v.33 no.16 pp. 4118-4125
ISSN:
0142-9612
Subject:
agarose; bioactive properties; biocompatible materials; cell culture; chitosan; endothelial cells; extracellular matrix; fibroblasts; gels; laminin; mixing; neurons; peptides; salivary glands; tissue engineering; tissue scaffolds
Abstract:
... Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing l ...
DOI:
10.1016/j.biomaterials.2012.02.044

20. Macroalga Padina pavonica water extracts obtained by pressurized liquid extraction and microwave-assisted extraction inhibit hyaluronidase activity as shown by capillary electrophoresis

Author:
Syntia Fayad; Reine Nehmé; Mona Tannoury; Eric Lesellier; Chantal Pichon; Philippe Morin
Source:
Journal of chromatography 2017 v.1497 pp. 19-27
ISSN:
0021-9673
Subject:
Padina pavonica; ammonium acetate; bioactive properties; buffers; capillary electrophoresis; electroporation; extracellular matrix; hyaluronic acid; hyaluronoglucosaminidase; inhibitory concentration 50; laminar flow; macroalgae; median effective concentration; microwave treatment; mixing; monitoring; pH; supercritical fluid extraction; wavelengths
Abstract:
... Hyaluronidase degrades hyaluronic acid, the principal component of the extracellular matrix. Inhibition of this enzyme is thus expected to hinder skin aging. Brown alga Padina pavonica activity toward hyaluronidase was evaluated using capillary electrophoresis (CE)-based enzymatic assays. This green technique allows evaluation of the biological activity of the natural material in an economic manne ...
DOI:
10.1016/j.chroma.2017.03.033

21. Oxime Cross-Linked Alginate Hydrogels with Tunable Stress Relaxation

Author:
Héctor Sánchez-Morán; Armin Ahmadi; Bernhard Vogler; Kyung-Ho Roh
Source:
Biomacromolecules 2019 v.20 no.12 pp. 4419-4429
ISSN:
1526-4602
Subject:
B-lymphocytes; biocompatibility; biocompatible materials; calcium; catalysts; cations; crosslinking; cultured cells; encapsulation; environmental factors; extracellular matrix; gelation; geometry; hydrogels; mechanical properties; microbeads; mixing; mixing ratio; moieties; oxidation; pH; polymers; sodium alginate; stress relaxation; temperature; viscoelasticity
Abstract:
... Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM). Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation. We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone. By mixing th ...
DOI:
10.1021/acs.biomac.9b01100

22. Porous Alginate Hydrogel Functionalized with Virus as Three-Dimensional Scaffolds for Bone Differentiation

Author:
Luckanagul Jittima; Lee L. Andrew; Nguyen Quyen L.; Sitasuwan Pongkwan; Yang Xiaoming; Shazly Tarek; Wang Qian
Source:
Biomacromolecules 2012 v.13 no.12 pp. 3949-3958
ISSN:
1526-4602
Subject:
Tobacco mosaic virus; alginates; biocompatible materials; capsid; cell differentiation; extracellular matrix; hydrogels; medicine; mixing; mutants; porous media; stem cells; tissues; virion; viruses
Abstract:
... In regenerative medicine, a synthetic extracellular matrix is crucial for supporting stem cells during its differentiation process to integrate into surrounding tissues. Hydrogels are used extensively in biomaterials as synthetic matrices to support the cells. However, to mimic the biological niche of a functional tissue, various chemical functionalities are necessary. We present here, a method of ...
DOI:
10.1021/bm301180c

23. Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Author:
Goktas Melis; Cinar Goksu; Orujalipoor Ilghar; Ide Semra; Tekinay Ayse B.; Guler Mustafa O.
Source:
Biomacromolecules 2015 v.16 no.4 pp. 1247-1258
ISSN:
1526-4602
Subject:
bioactive properties; crosslinking; epitopes; extracellular matrix; hydrogels; ligands; mechanical properties; medicine; mixing; nanofibers; polyethylene glycol; porosity; tissue engineering
Abstract:
... Natural extracellular matrix (ECM) consists of complex signals interacting with each other to organize cellular behavior and responses. This sophisticated microenvironment can be mimicked by advanced materials presenting essential biochemical and physical properties in a synergistic manner. In this work, we developed a facile fabrication method for a novel nanofibrous self-assembled peptide amphip ...
DOI:
10.1021/acs.biomac.5b00041

24. Self-healing hyaluronic acid hydrogels based on dynamic Schiff base linkages as biomaterials

Author:
Shangzhi Li; Minjie Pei; Tingting Wan; Hongjun Yang; Shaojin Gu; Yongzhen Tao; Xin Liu; Yingshan Zhou; Weilin Xu; Pu Xiao
Source:
Carbohydrate polymers 2020 v.250 pp. 116922
ISSN:
0144-8617
Subject:
biocompatibility; biocompatible materials; biodegradability; extracellular matrix; gelation; hyaluronic acid; hydrogels; imines; mechanical properties; mixing; pH; schiff bases
Abstract:
... Natural hydrogels are widely investigated for biomedical applications because of their structures similar to extracellular matrix of native tissues, possessing excellent biocompatibility and biodegradability. However, they are often susceptible to mechanical disruption. In this study, self-healing hyaluronic acid (HA) hydrogels are fabricated through a facile dynamic covalent Schiff base reaction. ...
DOI:
10.1016/j.carbpol.2020.116922

25. Supported Lipid Bilayers at Skeletonized Surfaces for the Study of Transmembrane Proteins

Author:
Fabre Roxane M.; Okeyo George O.; Talham Daniel R.
Source:
Langmuir 2012 v.28 no.5 pp. 2835-2841
ISSN:
1520-5827
Subject:
coatings; denaturation; extracellular matrix; fibronectins; integrins; lipid bilayers; lipids; mixing; models; solvents; transmembrane proteins; viability; zirconium
Abstract:
... Skeletonized zirconium phosphonate surfaces are used to support planar lipid bilayers and are shown to be viable substrates for studying transmembrane proteins. The skeletonized surfaces provide space between the bilayer and the solid support to enable protein insertion and avoid denaturation. The skeletonized zirconium octadecylphosphonate surfaces were prepared using Langmuir–Blodgett techniques ...
DOI:
10.1021/la204485n

26. The phenotypic response of bovine corneal endothelial cells on chitosan/polycaprolactone blends

Author:
Tsung-Jen Wang; I-Jong Wang; Shi Chen; Yi-Hsin Chen; Tai-Horng Young
Source:
Colloids and surfaces 2012 v.90 no. pp. 236-243
ISSN:
0927-7765
Subject:
adhesion; bioactive properties; biocompatible materials; biodegradability; cadherins; cattle; chitosan; collagen; colloids; cornea; endothelial cells; endothelium; extracellular matrix; gene expression; mixing; opacity; phenotype; tight junctions; tissue engineering
Abstract:
... Although various behaviors of corneal endothelial cells (CECs) have been investigated, the interaction of CECs with different biodegradable biomaterials has not been systematically well explored. Thus, two common biodegradable biomaterials with dissimilar characteristics, chitosan and polycaprolactone (PCL), were examined in bovine CEC (BCEC) culture systems to elucidate their possible impact on c ...
DOI:
10.1016/j.colsurfb.2011.10.043