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- Author:
- Lijun Zhao; Feixia Duan; Meng Gong; Xue Tian; Yan Guo; Lirong Jia; Sha Deng
- Source:
- Journal of agricultural and food chemistry 2021 v.69 no.11 pp. 3496-3510
- ISSN:
- 1520-5118
- Subject:
- Bacillus cereus; biofilm; diketopiperazines; enzyme activity; exopolysaccharides; extracellular matrix; food chemistry; food pathogens; gene expression; growth retardation; monoterpenoids; phenotype; spore germination
- Abstract:
- ... Bacillus cereus is a Gram-positive endospore-forming foodborne pathogen that causes lethal food poisoning and significant economic losses, usually through biofilm- and endospore-induced recurrent cross- and postprocessing contamination. Due to the lack of critical inhibitory targets and control strategies, B. cereus biofilm contamination is a problem that urgently needs a solution. In this study, ...
- DOI:
- 10.1021/acs.jafc.0c07826
- https://doi.org/10.1021/acs.jafc.0c07826
- Author:
- Yan Yong-Ming; Ai Jun; Shi Yan-Ni; Zuo Zhi-Li; Hou Bo; Luo Jie; Cheng Yong-Xian
- Source:
- Organic letters 2014 v.16 no.2 pp. 532-535
- ISSN:
- 1523-7052
- Subject:
- Aspongopus; enantiomers; extracellular matrix; insects; organic compounds; phosphorylation; rats; spectroscopy; transforming growth factor beta 1
- Abstract:
- ... (±)-Aspongamide A (1), an unusual trimer of N-acetyldopamine (NADA) bearing a novel tetrahydrobenzo[a]dibenzo[b,e][1,4]dioxine structure, and a pair of NADA dimeric enantiomers (2) were isolated from Aspongopus chinensis. The structures of compounds 1 and 2 were assigned using spectroscopic methods. Compound 1 was found to be an inhibitor of Smad3 phosphorylation in transforming growth factor-β1 ( ...
- DOI:
- 10.1021/ol403409v
- https://doi.org/10.1021/ol403409v
- Author:
- Bin Huang; Huangqin Chen
- Source:
- RSC advances 2015 v.5 no.30 pp. 23758-23766
- ISSN:
- 2046-2069
- Subject:
- Western blotting; acetic acid; animal models; colorimetry; dose response; enzyme-linked immunosorbent assay; epigallocatechin gallate; extracellular matrix; fluorometry; immunohistochemistry; interleukin-1beta; luciferase; macrophages; metalloproteinases; quantitative polymerase chain reaction; transcription (genetics)
- Abstract:
- ... The overexpression of matrix metalloproteinases (MMPs) results in excessive extracellular matrix degradation and oral ulcer healing delay. This study investigated the inhibitory effect of (−)-epigallocatechin-3-gallate (EGCG) on MMPs, the possible molecular mechanism and its implications for oral ulcers. MMP-1 and MMP-9 inhibition was detected using fluorometric and colorimetric assays after incub ...
- DOI:
- 10.1039/c5ra01263e
- https://doi.org/10.1039/c5ra01263e
- Author:
- Ilekuttige Priyan Shanura Fernando; Soo-Jin Heo; Mawalle Kankanamge Hasitha Madhawa Dias; Dissanayaka Mudiyanselage Dinesh Madusanka; Eui-Jeong Han; Min-Ju Kim; Kalu Kapuge Asanka Sanjeewa; Kyounghoon Lee; Ginnae Ahn
- Source:
- Marine drugs 2021 v.19 no.8 pp. -
- ISSN:
- 1660-3397
- Subject:
- Sargassum horneri; apoptosis; collagenase; elastase; extracellular matrix; fibroblasts; humans; ingredients; mitochondria; mitogen-activated protein kinase; necrosis; neoplasms; reactive oxygen species
- Abstract:
- ... Ultraviolet (UV) B exposure is a prominent cause of skin aging and a contemporary subject of interest. The effects are progressing through the generation of reactive oxygen species (ROS) that alter cell signaling pathways related to inflammatory responses. The present study evaluates the protective effects of (7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one (HTT) isolated from th ...
- DOI:
- 10.3390/md19080435
- https://doi.org/10.3390/md19080435
- Author:
- Victoria Keros; Ulrik Kvist
- Source:
- Cryobiology 2013 v.67 no.3 pp. 407
- ISSN:
- 0011-2240
- Subject:
- Leydig cells; Sertoli cells; adolescence; adults; biopsy; cooling; cryopreservation; cryoprotectants; extracellular matrix; freezing; histology; hospitals; humans; ice; intracytoplasmic sperm injection; liquids; medicine; men; neoplasms; nitrogen; patients; puberty; sowing; spermatogenesis; spermatozoa; storage time; straw; temperature; transmission electron microscopy; vitrification; Sweden
- Abstract:
- ... Cryopreservation of ejaculated or testicular spermatozoa is recommended for fertility preservation in adolescence and adult men. However cryobanking of the properly preserved human testicular tissue in men of reproductive age is an essential source for further clinical application and additional examination for diagnostic purposes. During the last three years 107 infertile patients were referred f ...
- DOI:
- 10.1016/j.cryobiol.2013.09.038
- https://doi.org/10.1016/j.cryobiol.2013.09.038
- Author:
- Madeline Sheeley; Dorothy Teegarden; Katie Wong
- Source:
- Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
- ISSN:
- 2475-2991
- Subject:
- breast neoplasms; breasts; cell viability; colorimetry; death; diagnostic techniques; energy metabolism; epithelial cells; ethanol; extracellular matrix; gene expression; genes; glutamine; human cell lines; messenger RNA; metabolites; metastasis; neoplasm cells; nutrient content; oxaloacetic acid; pyruvate carboxylase; quantitative polymerase chain reaction; triacylglycerols; tricarboxylic acid cycle; vitamin D
- Abstract:
- ... Metastasis is the main cause of most breast cancer related deaths. Throughout the process of metastasis, cancer cells strive to survive in conditions where they are detached from the extracellular matrix (ECM). Vitamin D may play a role in preventing breast cancer metastasis by modulating metabolism in breast cancer cells detached from ECM. Here we hypothesize that the active metabolite of vitamin ...
- DOI:
- 10.1093/cdn/nzz030.FS13-05-19
- https://doi.org/10.1093/cdn/nzz030.FS13-05-19
7. 111 Cryopreservation of stem cells inside alginate 3D constructs after high-voltage encapsulation
- Author:
- O. Gryshkov; D. Pogozhykh; N. Hofmann; T. Mueller; B. Glasmacher
- Source:
- Cryobiology 2013 v.67 no.3 pp. 429
- ISSN:
- 0011-2240
- Subject:
- Callithrix jacchus; air flow; alginates; analysis of variance; biochemical pathways; cooling; cryopreservation; cryoprotectants; electric potential difference; electrospraying; encapsulation; extracellular matrix; gels; heat transfer; mass transfer; medicine; mesenchymal stromal cells; microbeads; monkeys; sodium citrate; stem cells; storage temperature; storage time; surface area; thawing; toxicity; toxicity testing; viability; viscosity; water content
- Abstract:
- ... Application of stem cells in regenerative medicine require improving cryopreservation protocols for long term storage, especially for tissue-imitating 3D constructs containing cells. Encapsulation of stem cells into alginate-based 3D constructs that repeat an extracellular matrix may provide a mild environment during cryopreservation as well as the possibility for large-scale expansion. Furthermor ...
- DOI:
- 10.1016/j.cryobiol.2013.09.117
- https://doi.org/10.1016/j.cryobiol.2013.09.117
- Author:
- M. Hamdi; B. Rodríguez-Alonso; A. Almansa-Ordonez; A. Gutierrez-Adán; P. Lonergan; D. Rizos
- Source:
- Reproduction, fertility, and development 2018 v.30 no.1 pp. 197-198
- ISSN:
- 1031-3613
- Subject:
- air; analysis of variance; carbon dioxide; cattle; coculture; cytokinesis; direct contact; epithelial cells; essential genes; extracellular matrix; fetal bovine serum; gene expression; indirect contact; inflammation; oviducts; oxidative stress; polyesters; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; sodium; somatomedins; transcriptome; transcriptomics
- Abstract:
- ... We observed that in vitro transcriptomic response of bovine oviduct epithelial cells (BOEC) to the early embryo could be the result of a contact-dependent signalling effect or interactions with embryo secretions. In order to determine this, BOEC were co-cultured directly with embryos or indirectly with embryo-conditioned media (CM); BOEC from the isthmus of oviducts at early luteal phase were cult ...
- DOI:
- 10.1071/RDv30n1Ab115
- https://doi.org/10.1071/RDv30n1Ab115
- Author:
- Boris Rubinsky
- Source:
- Cryobiology 2015 v.71 no.1 pp. 167-168
- ISSN:
- 0011-2240
- Subject:
- cell membranes; research; fish; heart; extracellular matrix; freezing; transportation; glucose; mechanical damage; solidification; ice; antifreeze proteins; frogs; glycerol; temperature; Lithobates sylvaticus; liver; cryopreservation; cold tolerance; thawing; Arctic region; Antarctic region
- Abstract:
- ... Star Wars V: The Empire Strikes Back – One of the most dramatic scenes in the movie is when the Dark Lord successfully entombs Han Solo in carbonite in a state of suspended animation for transportation to Tatooine to receive a bounty from Jabba. Recently records show that the film has made so far $819,808,300. I doubt that the entire scientific community, together, through the last century, has re ...
- DOI:
- 10.1016/j.cryobiol.2015.05.018
- https://doi.org/10.1016/j.cryobiol.2015.05.018
- Author:
- E. Derisoud; L. Jouneau; C. Archilla; N. Daniel; Y. Jaszczyszyn; C. Gourtay; A. Margat; M. Dahirel; N. Peynot; L. Briot; F. De Geoffroy; V. Duranthon; P. Chavatte-Palmer
- Source:
- Reproduction, fertility, and development 2021 v.33 no.2 pp. 179
- ISSN:
- 1031-3613
- Subject:
- DNA; RNA; embryonic mortality; extracellular matrix; foals; focal adhesions; gene expression; gene expression regulation; gene ontology; genes; immune response; mares; models; myosin; nucleosomes; plasma membrane; proteasome endopeptidase complex; protein metabolism; ribosomes; semen; sphingolipids; stallions; trophoblast
- Abstract:
- ... An increased incidence in early embryo loss has been observed in aged mares. Moreover, the first foal born to a mare is lighter than her subsequent foals, with reported impaired placental function at term. Because trophoblast function may be affected from the embryo stage, the aim of this project was to determine the effect of parity in aged mares on gene expression in Day-8.5 embryos. Middle-aged ...
- DOI:
- 10.1071/RDv33n2Ab142
- https://doi.org/10.1071/RDv33n2Ab142
- Author:
- Haidong Wang; Zhaohui Li; Yachong Huo; Tao Tian; Dalong Yang; Lei Ma; Sidong Yang; Wenyuan Ding
- Source:
- Life sciences 2021 v.284 pp. 119874
- ISSN:
- 0024-3205
- Subject:
- X-radiation; antagonists; apoptosis; blood serum; eosin; estrogen receptors; extracellular matrix; fluorescent antibody technique; immunohistochemistry; intervertebral disks; magnetism; metalloproteinases; models; ovariectomy; rats; signal transduction
- Abstract:
- ... To investigate the effect of 17β-Estradiol (E2) on intervertebral disc degeneration (IVDD) and the related mechanism.Immunohistochemistry was used to detect the expression of estrogen receptor β (ERβ) within intervertebral discs of humans and rats. After that, rat IVDD model was established by needle puncture and bilateral ovariectomy. Then, the serum E2 level was detected by enzyme linked immunos ...
- DOI:
- 10.1016/j.lfs.2021.119874
- https://doi.org/10.1016/j.lfs.2021.119874
- Author:
- L. R. Padoveze; M. Rubessa; C. E. Ambrosio; M. B. Wheeler
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 217-218
- ISSN:
- 1031-3613
- Subject:
- alizarin; bone formation; calcium; extracellular matrix; gene expression regulation; genes; in vitro studies; linear models; magnesium; magnesium sulfate; mesenchymal stromal cells; mineralization; staining; stem cells; swine; tissue engineering
- Abstract:
- ... Tissue engineering offers a viable alternative to bone grafts in repairing large bone defects. Magnesium-based materials are biocompatible in vivo, and it is possible to determine the degradation period according to the necessities (Farraro et al. 2014 J. Biomech. 47, 1979-1986). Magnesium (Mg) is part of many physiological processes, and it promotes the osteogenesis of mesenchymal stem cells (Día ...
- DOI:
- 10.1071/RDv31n1Ab186
- https://doi.org/10.1071/RDv31n1Ab186
- Author:
- Boda Chen; Dingchao Zhu; Chenglong Xie; Yifeng Shi; Libin Ni; Huawei Zhang; Sunlong Li; Jiajie Lu; Jian Xiao; Weiyi Xia; Chongan Huang; Xiangyang Wang
- Source:
- Food & function 2021 v.12 no.18 pp. 8399-8410
- ISSN:
- 2042-650X
- Subject:
- Glycyrrhiza glabra; basic-leucine zipper transcription factors; bioactive compounds; cartilage; chondrocytes; collagen; extracellular matrix; histology; inducible nitric oxide synthase; inflammation; interleukin-6; matrix metalloproteinase 13; mice; necrosis; neoplasms; nitric oxide; osteoarthritis; prostaglandin synthase; prostaglandins; sclerosis; synovitis; therapeutics; transcription factor NF-kappa B
- Abstract:
- ... Osteoarthritis (OA) is presently the most prevalent form of chronic degenerative joint disease, which is characterized by erosion of articular cartilage, subchondral bone sclerosis and synovitis. Accumulating evidence has revealed that 18β-glycyrrhetinic acid (18β-GA), a major bioactive component derived from Glycyrrhiza glabra, exerts anti-inflammatory effects on several diseases. However, the an ...
- DOI:
- 10.1039/d1fo01379c
- https://doi.org/10.1039/d1fo01379c
- Author:
- Jennifer C. Wilson; Andrew Elohim Laloo; Sanjesh Singh; Vito Ferro
- Source:
- Biochemical and biophysical research communications 2014 v.443 pp. 185-188
- ISSN:
- 0006-291X
- Subject:
- angiogenesis; anti-inflammatory agents; extracellular matrix; glycosides; heparan sulfate; hydrolysis; inflammation; metastasis; mutagenicity; nuclear magnetic resonance spectroscopy; prediction; proteoglycans; sequence analysis; spectral analysis
- Abstract:
- ... Heparanase is an endo-β-glucuronidase that cleaves heparan sulfate side chains of proteoglycans in basement membranes and the extracellular matrix (ECM). Heparanase is implicated in several diverse pathological processes associated with ECM degradation such as metastasis, inflammation and angiogenesis and is thus an important target for anti-cancer and anti-inflammatory drug discovery. Heparanase ...
- DOI:
- 10.1016/j.bbrc.2013.11.079
- https://doi.org/10.1016/j.bbrc.2013.11.079
- Author:
- V.J. Woeckel; C. Bruedigam; M. Koedam; H. Chiba; B.C.J. van der Eerden; J.P.T.M. van Leeuwen
- Source:
- Gene 2013 v.512 pp. 438-443
- ISSN:
- 0378-1119
- Subject:
- agonists; alkaline phosphatase; extracellular matrix; gene expression; genes; humans; mineralization; osteoblasts; transcription factors; vitamin D
- Abstract:
- ... Both vitamin D receptor (VDR) and peroxisome proliferator-activated receptor γ (PPAR-γ) are ligand-activated nuclear transcription factors that are instrumental for bone health. While 1α,25-dihydroxyvitamin D3 (1,25D3), the ligand for VDR, is essential for the development and maintenance of healthy bone, PPAR-γ agonists cause detrimental skeletal effects. Recent studies have revealed evidence for ...
- DOI:
- 10.1016/j.gene.2012.07.051
- PubMed:
- 22967709
- https://doi.org/10.1016/j.gene.2012.07.051
- Author:
- Fei Jiang; Yong Yang; Lian Xue; Bingyan Li; Zengli Zhang
- Source:
- Nutrients 2017 v.9 no.9 pp. -
- ISSN:
- 2072-6643
- Subject:
- beta catenin; blood serum; cadherins; calcitriol receptors; cell movement; collagen; epidemiological studies; epithelial cells; epithelium; extracellular matrix; fibronectins; fibrosis; genes; humans; lungs; phenotype; prognosis; respiratory tract diseases; tissue repair; transcription factors; transforming growth factor beta; vimentin; vitamin D
- Abstract:
- ... Pulmonary fibrosis is a progressive fibrotic lung disease of persisting lung injury and ineffective wound repair, with poor prognosis. Epithelial–mesenchymal transition (EMT) of alveolar epithelia cells is an early event in the development of pulmonary fibrosis, and transforming growth factor β (TGF-β) is an acknowledged inducer of EMT. Epidemiological studies demonstrated that serum levels of 25- ...
- DOI:
- 10.3390/nu9090980
- PubMed:
- 28878195
- PubMed Central:
- PMC5622740
- https://doi.org/10.3390/nu9090980
- Author:
- Yingjuan Lv; Xiaohui Han; Qingbin Yao; Kun Zhang; Lina Zheng; Wei Hong; Xiaoli Xing
- Source:
- Biochemical and biophysical research communications 2019 v.516 no.1 pp. 75-81
- ISSN:
- 0006-291X
- Subject:
- Primates; apoptosis; biochemical mechanisms; calcitriol receptors; cholecalciferol; extracellular matrix; glaucoma; humans; hydrogen peroxide; inflammation; models; oxidative stress; therapeutics; transforming growth factor beta
- Abstract:
- ... Evidence indicates that 1α, 25-dihydroxy vitamin D3 (1, 25-(OH)2D3) markedly reduces intraocular pressure (IOP) in nonhuman primates, while the biochemical mechanisms are unclear. To investigate the influence of oxidative stress on human trabecular meshwork cells (HTMCs) and the effect and regulatory mechanism of 1, 25-(OH)2D3 in HTMCs under oxidative stress, we established an oxidative stress mod ...
- DOI:
- 10.1016/j.bbrc.2019.06.027
- https://doi.org/10.1016/j.bbrc.2019.06.027
- Author:
- Paulomi Ghosh; Arun Prabhu Rameshbabu; Nantu Dogra; Santanu Dhara
- Source:
- RSC advances 2014 v.4 no.37 pp. 19516-19524
- ISSN:
- 2046-2069
- Subject:
- adhesion; adsorption; alkaline phosphatase; animal models; bone formation; bone marrow; calcium; cell proliferation; chemical bonding; chitosan; crosslinking; extracellular matrix; femur; glucosamine; histology; humans; hydrophobicity; imines; in vivo studies; mesenchymal stromal cells; neutralization; osteocalcin; pH; polymers; rabbits; secretion; sodium hydroxide; tensile strength; tissue engineering
- Abstract:
- ... Chitosan fibers were fabricated via pH induced neutralization and precipitation in a 5 w/v% NaOH bath. Intermolecular covalent crosslinking of these fibers were performed through imine linkages between the glucosamine units of polymers and the dialdehyde groups of 2,5-dimethoxy-2,5-dihydrofuran at 60 °C, pH 2.2. The covalently crosslinked fibers demonstrated improved tensile strength, stiffness, h ...
- DOI:
- 10.1039/c4ra01971g
- https://doi.org/10.1039/c4ra01971g
- Author:
- Yuanyuan Song; Ziqi Wang; Yijing Long; Yang Mao; Feng Jiang; Yuanyuan Lu
- Source:
- Research in microbiology 2022 pp. 103955
- ISSN:
- 0923-2508
- Subject:
- Candida albicans; anthraquinones; biofilm; extracellular matrix; glycosides; hyphae; microbiology; research; transcriptomics; virulence
- Abstract:
- ... Candida albicans can form biofilm on biotic and abiotic surfaces of medical implants to cause superficial and systemic infections under specific condition. The formation of hyphae and matrix of C. albicans are considered as probable virulence factors. We assessed the inhibitory activities of 26 anthraquinones against C. albicans biofilm formation, which were substituted by different functional gro ...
- DOI:
- 10.1016/j.resmic.2022.103955
- https://doi.org/10.1016/j.resmic.2022.103955
- Author:
- Chunxue Li; Yating Zhan; Rongrong Zhang; Qiqi Tao; Zhichao Lang; Jianjian Zheng
- Source:
- Journal of Ginseng Research 2022 pp. -
- ISSN:
- 1226-8453
- Subject:
- DNA methyltransferase; Panax; carbon tetrachloride; collagen; demethylation; drugs; extracellular matrix; liver; liver cirrhosis; methylation; research; therapeutics
- Abstract:
- ... 20(S)-protopanaxadiol (PPD), one of the main components of ginseng, has anti-inflammatory, anti-estrogenic, and anti-tumor activities. It is known that activated hepatic stellate cells (HSCs) are the primary producers of extracellular matrix (ECM) in the liver, and the Wnt/β-catenin pathway participates in the activation of HSCs. We aimed to explore whether PPD inhibits liver fibrosis is associate ...
- DOI:
- 10.1016/j.jgr.2022.05.005
- https://doi.org/10.1016/j.jgr.2022.05.005
- Author:
- Jian Song; Zhen-Yu Cui; Li-Hua Lian; Xin Han; Li-Shuang Hou; Ge Wang; Lu Gao; Yue Zhu; Yu-Chen Jiang; Jia-Yi Dou; Zhong-He Hu; Yu-Qing Zhao; Ji-Xing Nan; Yan-Ling Wu
- Source:
- Journal of agricultural and food chemistry 2020 v.68 no.31 pp. 8195-8204
- ISSN:
- 1520-5118
- Subject:
- Panax; agonists; caspase-1; extracellular matrix; fibrosis; food chemistry; functional foods; histopathology; inflammation; interleukin-6; ion channels; liver cirrhosis; metabolites; protective effect; secretion
- Abstract:
- ... Ginseng has been used as a functional food and tonic for enhancing immune power. Here, the potential protective effect of 20S-protopanaxatriol (M4), the metabolite of protopanaxatriol, against hepatic fibrosis is investigated, which could provide nutritional interventions for disease treatment. M4 could inhibit extracellular matrix (ECM) deposition and reduce the levels of proinflammatory cytokine ...
- DOI:
- 10.1021/acs.jafc.0c01978
- https://doi.org/10.1021/acs.jafc.0c01978
- Author:
- L. S. Machado; N. C. G. Pieri; R. C. Botigelli; R. V. G. Castro; A. F. de Souza; M. A. de Lima; A. Bridi; A. F. C. Andrade; F. V. Meirelles; K. K. Freude; F. F. Bressan
- Source:
- Reproduction, fertility, and development 2020 v.32 no.2 pp. 236-237
- ISSN:
- 1031-3613
- Subject:
- Parkinson disease; alkaline phosphatase; animal models; antibiotics; autism; beta-mercaptoethanol; blood serum; cytoplasm; epidermal growth factor; extracellular matrix; fibroblast growth factor 2; fibroblasts; flow cytometry; fluorescent antibody technique; genes; genetic disorders; glutamine; human diseases; humans; induced pluripotent stem cells; medicine; neurons; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; schizophrenia; swine; translational medical research; tubulin; vimentin
- Abstract:
- ... The invitro cellular induction into pluripotency [induced pluripotent stem cell (iPSC) generation] has much evolved since its first report and may play a central role in veterinary regenerative medicine. In particular, the generation of invitro neural precursor cells (NPCs) enabled the creation of models for human genetic diseases, such as autism, schizophrenia, and Parkinson disease. The swine mo ...
- DOI:
- 10.1071/RDv32n2Ab217
- https://doi.org/10.1071/RDv32n2Ab217
- Author:
- Mike Taylor; Kelvin G.M. Brockbank; Yoed Rabin
- Source:
- Cryobiology 2015 v.71 no.1 pp. 170-171
- ISSN:
- 0011-2240
- Subject:
- additives; blood cells; blood vessels; cartilage; cell suspension culture; cooling; cryopreservation; cryoprotectants; crystallization; crystals; dimethyl sulfoxide; extracellular matrix; freezing; heart valves; heat treatment; ice; ice nucleation; islets of Langerhans; kidneys; liquids; mechanical stress; risk; solidification; solutes; stem cells; temperature; toxicity; viscosity; vitrification
- Abstract:
- ... Cryopreservation involving freezing in the presence of protective agents such as dimethyl sulfoxide is a well-established method for storing cells and small tissues. While successful techniques for cryopreservation have been developed over the past seven decades, they are generally related to small specimens, in the scale-range of cell suspensions and clusters to small-organized tissues (μm to mm ...
- DOI:
- 10.1016/j.cryobiol.2015.05.028
- https://doi.org/10.1016/j.cryobiol.2015.05.028
- Author:
- S. Johnson; D. Milner; H. Lopez-Lake; M. Wheeler
- Source:
- Reproduction, fertility, and development 2016 v.28 no.2 pp. 254
- ISSN:
- 1031-3613
- Subject:
- adults; cartilage; cell nucleus; cell proliferation; centrifugation; chondrocytes; chondrogenesis; chondroitin sulfate; collagen; computer software; dexamethasone; extracellular matrix; fetal bovine serum; image analysis; keratin; pellets; proline; stem cells; sulfates; swine; t-test; tissue engineering
- Abstract:
- ... Pellet cultures are commonly used to study chondrogenic differentiation in vitro. Our laboratory has demonstrated pellets made with chondrocytes grow in size during culture and produce cartilage matrix, but pellets made with adipose-derived mesenchymal stem cells (ASC) grow only slightly, producing little cartilage matrix. The objective of this study was to determine if differences in chondrocyte ...
- DOI:
- 10.1071/RDv28n2Ab244
- https://doi.org/10.1071/RDv28n2Ab244
- Author:
- I-Hua Chen; Fang-Rong Chang; Yang-Chang Wu; Po-Hsiung Kung; Chin-Chung Wu
- Source:
- Biochimie 2015 v.110 pp. 81-92
- ISSN:
- 0300-9084
- Subject:
- CD29 antigen; Western blotting; actin; adhesion; antineoplastic agents; breast neoplasms; cell adhesion; cell viability; confocal microscopy; extracellular matrix; immigration; metastasis; neoplasm cells; non-specific protein-tyrosine kinase; phosphorylation; protein disulfide-isomerase; surface proteins; thiols
- Abstract:
- ... Triple negative breast cancer (TNBC) exhibits an aggressive clinical course by high metastatic potential. It is known that integrin-mediated cell adhesion and migration are important for cancer metastasis. In the present study, a synthetic compound, 3, 4-methyenedioxy-β-nitrostyrene (MNS), significantly inhibited adhesion of TNBC cell lines to different extracellular matrix (ECM) components. The a ...
- DOI:
- 10.1016/j.biochi.2015.01.006
- https://doi.org/10.1016/j.biochi.2015.01.006
- Author:
- Jingjing Luo; Haitao Zhang; Jiang zhu; Xiaokang Cui; Junjiu Gao; Xin Wang; Jie Xiong
- Source:
- Colloids and surfaces 2018 v.163 pp. 369-378
- ISSN:
- 0927-7765
- Subject:
- Fourier transform infrared spectroscopy; X-ray diffraction; binding properties; bone formation; bones; colloids; extracellular matrix; fibroins; gene expression; genes; glutamic acid; messenger RNA; mineralization; nanofibers; scanning electron microscopy; silk; stem cells; tissue engineering
- Abstract:
- ... In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated wit ...
- DOI:
- 10.1016/j.colsurfb.2017.12.043
- https://doi.org/10.1016/j.colsurfb.2017.12.043
- Author:
- K. Jiang; D. Chaimov; S.N. Patel; J.-P. Liang; S.C. Wiggins; M.M. Samojlik; A. Rubiano; C.S. Simmons; C.L. Stabler
- Source:
- Biomaterials 2019 v.198 pp. 37-48
- ISSN:
- 0142-9612
- Subject:
- bladder; cell viability; cost effectiveness; diabetes; encapsulation; endothelial cells; extracellular matrix; humans; hydrogels; islets of Langerhans; lungs; organ-on-a-chip; physiology; rats; screening; swine; viscoelasticity
- Abstract:
- ... Organ-on-a-chip platforms serve as cost-efficient testbeds for screening pharmaceutical agents, mimicking natural physiology, and studying disease. In the field of diabetes, the development of an islet-on-a-chip platform would have broad implications in understanding disease pathology and discovering potential therapies. Islet microphysiological systems are limited, however, by their poor cell sur ...
- DOI:
- 10.1016/j.biomaterials.2018.08.057
- PubMed:
- 30224090
- PubMed Central:
- PMC6397100
- https://doi.org/10.1016/j.biomaterials.2018.08.057
- Author:
- Narges Naseri; Jean-Michel Poirier; Lenart Girandon; Mirjam Fröhlich; Kristiina Oksman; Aji P. Mathew
- Source:
- RSC advances 2016 v.6 no.8 pp. 5999-6007
- ISSN:
- 2046-2069
- Subject:
- cartilage; cellulose nanofibers; chitosan; chondrocytes; chondrogenesis; computed tomography; crosslinking; extracellular matrix; freeze drying; gelatin; mechanical properties; nanocomposites; phosphates; porosity; roughness; scanning electron microscopy; tissue engineering
- Abstract:
- ... Fully bio-based 3-dimensional porous scaffolds based on freeze-dried cellulose nanofibers (70–90 wt%) stabilized using a genipin crosslinked matrix of gelatin and chitosan were prepared. Morphology studies using scanning electron microscopy showed that the scaffolds have interconnected pores with average pore diameters of 75–200 μm and nanoscaled pore wall roughness, both favorable for cell intera ...
- DOI:
- 10.1039/c5ra27246g
- https://doi.org/10.1039/c5ra27246g
- Author:
- Daniel S. Puperi; Liezl R. Balaoing; Ronan W. O'Connell; Jennifer L. West; K. Jane Grande-Allen
- Source:
- Biomaterials 2015 v.67 pp. 354-364
- ISSN:
- 0142-9612
- Subject:
- cell growth; coculture; direct contact; disease course; endothelial cells; extracellular matrix; heart valve diseases; hydrocolloids; ligands; models; phenotype
- Abstract:
- ... Physiologically relevant in vitro models are needed to study disease progression and to develop and screen potential therapeutic interventions for disease. Heart valve disease, in particular, has no early intervention or non-invasive treatment because there is a lack of understanding the cellular mechanisms which lead to disease. Here, we establish a novel, customizable synthetic hydrogel platform ...
- DOI:
- 10.1016/j.biomaterials.2015.07.039
- PubMed:
- 26241755
- PubMed Central:
- PMC4566924
- https://doi.org/10.1016/j.biomaterials.2015.07.039
- Author:
- Jeunghwan Choi; Michael Etheridge; Dushyant Mehra; John Bischof
- Source:
- Cryobiology 2013 v.66 no.3 pp. 350
- ISSN:
- 0011-2240
- Subject:
- atrial fibrillation; catheters; cryosurgery; databases; differential scanning calorimetry; energy; extracellular matrix; gels; glass transition; glass transition temperature; heat transfer; ice; latent heat; models; myocardium; nerve tissue; prediction; risk reduction; specific heat; swine; thermal conductivity; thermistors; thrombosis; water
- Abstract:
- ... Cryoablation for atrial fibrillation is a clinically approved technique used to control irregular heart rhythms due to abnormal electrical signals conducted from the pulmonary veins to the atria. The ablation catheter rapidly cools the cardiac tissue to freezing temperatures creating a discrete lesion which terminates these abnormal electrical pulses. Benefits of this approach include decreased ri ...
- DOI:
- 10.1016/j.cryobiol.2013.02.036
- https://doi.org/10.1016/j.cryobiol.2013.02.036
- Author:
- Yogendra Pratap Singh; Ashutosh Bandyopadhyay; Biman B. Mandal
- Source:
- ACS applied materials & interfaces 2019 v.11 no.37 pp. 33684-33696
- ISSN:
- 1944-8252
- Subject:
- Bombyx mori; Philosamia; biocompatibility; bioprinting; cartilage; chondrocytes; collagen; compression strength; crosslinking; encapsulation; extracellular matrix; extrusion; fibroins; gelatin; gene expression; gene expression regulation; glycosaminoglycans; humans; hypertrophy; polymers; quality of life; rheology; silk; tissue engineering; toxic substances
- Abstract:
- ... Cartilage tissue is deprived of intrinsic self-regeneration capability; hence, its damage often progresses to a chronic condition which reduces the quality of life. Toward the fabrication of functional tissue substitutes, three-dimensional (3D) bioprinting has progressed vastly over the last few decades. However, this progress is challenged by the difficulty in developing suitable bioink materials ...
- DOI:
- 10.1021/acsami.9b11644
- https://doi.org/10.1021/acsami.9b11644
- Author:
- Qing-Qing Qiu; Paul Ducheyne; Portonovo S. Ayyaswamy
- Source:
- In vitro cellular & developmental biology 2001 v.37 no.3 pp. 157-165
- ISSN:
- 1071-2690
- Subject:
- apatite; biodegradability; bone marrow; cell culture; drug delivery systems; extracellular matrix; glass; in vitro studies; microgravity; mineralization; osteoblasts; polymers; stromal cells; tissue engineering
- Abstract:
- ... Three-dimensional (3D) osteoblast cell cultures were obtained in rotating-wall vessels (RWV), simulating microgravity. Three types of bioactive microcarriers, specifically modified bioactive glass particles, bioceramic hollow microspheres, and biodegradable bioactive glass-polymer composite microspheres, were developed and used with osteblasts. The surfaces of composite microspheres fully transfor ...
- DOI:
- 10.1290/1071-2690(2001)037<0157:BTEWBM>2.0.CO;2
- https://doi.org/10.1290/1071-2690(2001)037<0157:BTEWBM>2.0.CO;2
- Author:
- Raffaella Parente; Valentina Possetti; Maria Lucia Schiavone; Elisabetta Campodoni; Ciro Menale; Mattia Loppini; Andrea Doni; Barbara Bottazzi; Alberto Mantovani; Monica Sandri; Anna Tampieri; Cristina Sobacchi; Antonio Inforzato
- Source:
- Pathogens 2021 v.10 no.7 pp. -
- ISSN:
- 2076-0817
- Subject:
- Gram-positive bacteria; Staphylococcus aureus; animal experimentation; biomimetics; cell lines; coculture; collagen; extracellular matrix; gene expression; homeostasis; hydroxyapatite; immune response; infectious diseases; mice; models; opportunistic pathogens; osteoblasts; osteomyelitis; protein synthesis; texture; transcription (genetics)
- Abstract:
- ... Osteomyelitis (OM) is an infectious disease of the bone primarily caused by the opportunistic pathogen Staphylococcus aureus (SA). This Gram-positive bacterium has evolved a number of strategies to evade the immune response and subvert bone homeostasis, yet the underlying mechanisms remain poorly understood. OM has been modeled in vitro to challenge pathogenetic hypotheses in controlled conditions ...
- DOI:
- 10.3390/pathogens10070837
- https://doi.org/10.3390/pathogens10070837
- Author:
- Julien Nicolas; Sofia Magli; Linda Rabbachin; Susanna Sampaolesi; Francesco Nicotra; Laura Russo
- Source:
- Biomacromolecules 2020 v.21 no.6 pp. 1968-1994
- ISSN:
- 1526-4602
- Subject:
- biochemical compounds; chemistry; drugs; extracellular matrix; medicine; stem cells
- Abstract:
- ... Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tunin ...
- DOI:
- 10.1021/acs.biomac.0c00045
- https://doi.org/10.1021/acs.biomac.0c00045
- Author:
- Justyna Borowiec; Joerg Hampl; Sukhdeep Singh; Sebastian Haefner; Karin Friedel; Patrick Mai; Dana Brauer; Florian Ruther; Liliana Liverani; Aldo R. Boccaccini; Andreas Schober
- Source:
- ACS applied materials & interfaces 2018 v.10 no.26 pp. 22857-22865
- ISSN:
- 1944-8252
- Subject:
- adhesion; antibodies; cell culture; extracellular matrix; geometry; materials science; microstructure; proteins; staining
- Abstract:
- ... Micrometer-scale biochemical or topographical patterning is commonly used to guide the cell attachment and growth, but the ability to combine these patterns into an integrated surface with defined chemical and geometrical characteristics still remains a technical challenge. Here, we present a technical solution for simultaneous construction of 3D morphologies, in the form of channels, on porous me ...
- DOI:
- 10.1021/acsami.8b06585
- https://doi.org/10.1021/acsami.8b06585
- Author:
- Juan Chen; Tonghui Zhang; Weikang Hua; Peiyun Li; Xuefen Wang
- Source:
- Colloids and surfaces 2020 v.585 pp. 124048
- ISSN:
- 0927-7757
- Subject:
- apatite; bioactive properties; biomedical materials; biomimetics; biomineralization; bone formation; bones; cell structures; cellulose; citric acid; crosslinking; extracellular matrix; freeze drying; hydrophilicity; moieties; nanofibers; polylactic acid; strength (mechanics); tissue engineering; water uptake
- Abstract:
- ... One of the serious challenges in bone tissue engineering is the construction of biomimetic scaffolds, which can bridge the gap between mechanical strength and porous structure requirements. Although electrospinning technology can be used to create nanofibrous networks with a structure of artificial extracellular matrix (ECM), the restricted shapes and pore sizes block its application. Herein, we c ...
- DOI:
- 10.1016/j.colsurfa.2019.124048
- https://doi.org/10.1016/j.colsurfa.2019.124048
- Author:
- Chen Guobao; Dong Chanjuan; Yang Li; Lv Yonggang
- Source:
- ACS Applied Materials & Interfaces 2015 v.7 no.29 pp. 15790-15802
- ISSN:
- 1944-8252
- Subject:
- adhesion; angiogenesis; bone formation; bones; cell adhesion; cell differentiation; cell viability; coatings; collagen; extracellular matrix; hydroxyapatite; mechanical properties; micro-computed tomography; microstructure; osteoblasts; porosity; rats; stem cells; tissue engineering
- Abstract:
- ... A growing body of evidence has shown that extracellular matrix (ECM) stiffness can modulate stem cell adhesion, proliferation, migration, differentiation, and signaling. Stem cells can feel and respond sensitively to the mechanical microenvironment of the ECM. However, most studies have focused on classical two-dimensional (2D) or quasi-three-dimensional environments, which cannot represent the re ...
- DOI:
- 10.1021/acsami.5b02662
- https://doi.org/10.1021/acsami.5b02662
- Author:
- Jung Hyuntae; Park Ji Sun; Yeom Junseok; Selvapalam Narayanan; Park Kyeng Min; Oh Kyunghoon; Yang Jeong-A; Park Keun Hong; Hahn Sei Kwang; Kim Kimoon
- Source:
- Biomacromolecules 2014 v.15 no.3 pp. 707-714
- ISSN:
- 1526-4602
- Subject:
- cartilage; chondrogenesis; crosslinking; dexamethasone; extracellular matrix; glycosaminoglycans; humans; hydrogels; immunohistochemistry; mechanical properties; quantitative polymerase chain reaction; stem cells; tissue engineering; transforming growth factor beta 3
- Abstract:
- ... Despite a wide investigation of hydrogels as an artificial extracellular matrix, there are few scaffold systems for the facile spatiotemporal control of mesenchymal stem cells (MSCs). Here, we report 3D tissue engineered supramolecular hydrogels prepared with highly water-soluble monofunctionalized cucurbit[6]uril–hyaluronic acid (CB[6]-HA), diaminohexane conjugated HA (DAH-HA), and drug conjugate ...
- DOI:
- 10.1021/bm401123m
- https://doi.org/10.1021/bm401123m
- Author:
- Nathalie Picollet-D’hahan; Monika E. Dolega; Lavinia Liguori; Christophe Marquette; Séverine Le Gac; Xavier Gidrol; Donald K. Martin
- Source:
- Trends in biotechnology 2016 pp. -
- ISSN:
- 0167-7799
- Subject:
- engineering; animal use replacement; humans; organ-on-a-chip; drugs; models; cell harvesting; exocrine glands; screening; extracellular matrix
- Abstract:
- ... We discuss the current challenges and future prospects of flow-based organoid models and 3D self-assembling scaffolds. The existing paradigm of 3D culture suffers from a lack of control over organoid size and shape; can be an obstacle for cell harvesting and extended cellular and molecular analysis; and does not provide access to the function of exocrine glands. Moreover, existing organ-on-chip mo ...
- DOI:
- 10.1016/j.tibtech.2016.06.012
- https://doi.org/10.1016/j.tibtech.2016.06.012
- Author:
- Changxu Deng; Jin Yang; Hongtao He; Zhenjiang Ma; Wenhao Wang; Yuxin Zhang; Tao Li; Chuanglong He; Jinwu Wang
- Source:
- Biomaterials science 2021 v.9 no.14 pp. 4891-4903
- ISSN:
- 2047-4849
- Subject:
- anhydrides; biocompatible materials; bioprinting; cartilage; chemical bonding; chondrocytes; extracellular matrix; fibroins; gelatin; hypertrophy; models; parathyroid hormone; phenotype; rabbits
- Abstract:
- ... Repair of osteochondral defects is still a challenge, especially the regeneration of hyaline cartilage. Parathyroid hormone (PTH) can inhibit the hypertrophy of chondrocytes to maintain the phenotype of hyaline cartilage. Here, we aimed to construct a bio-printed biphasic scaffold with a mechanical gradient based on dual modification of silk fibroin (SF) for the integrated repair of osteochondral ...
- DOI:
- 10.1039/d1bm00535a
- https://doi.org/10.1039/d1bm00535a
- Author:
- Xifang Chen; Zhilian Yue; Pia C. Winberg; Yan-Ru Lou; Stephen Beirne; Gordon G. Wallace
- Source:
- Biomaterials science 2021 v.9 no.7 pp. 2424-2438
- ISSN:
- 2047-4849
- Subject:
- biocompatibility; biocompatible materials; bioprinting; cell proliferation; cell viability; collagen; elastin; extracellular matrix; extrusion; fibroblasts; fibronectins; gelatin; glycosaminoglycans; humans; macroalgae; strength (mechanics); three-dimensional printing; tissue repair
- Abstract:
- ... 3D bioprinting has been increasingly employed in skin tissue engineering for manufacturing living constructs with three-dimensional spatial precision and controlled architecture. There is however, a bottleneck in the tunability of bioinks to address specific biocompatibility challenges, functional traits and printability. Here we report on a traditional gelatin methacryloyl (GelMA) based bioink, t ...
- DOI:
- 10.1039/d0bm01784a
- https://doi.org/10.1039/d0bm01784a
- Author:
- Byoung Soo Kim; Yang Woo Kwon; Jeong-Sik Kong; Gyu Tae Park; Ge Gao; Wonil Han; Moon-Bum Kim; Hyungseok Lee; Jae Ho Kim; Dong-Woo Cho
- Source:
- Biomaterials 2018 v.168 pp. 38-53
- ISSN:
- 0142-9612
- Subject:
- angiogenesis; biocompatible materials; biofabrication; bioprinting; blood flow; cytokines; extracellular matrix; growth factors; manufacturing; mechanical properties; models; secretion; shrinkage; skin (animal); stem cells; tissue culture; tissue engineering
- Abstract:
- ... 3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rel ...
- DOI:
- 10.1016/j.biomaterials.2018.03.040
- https://doi.org/10.1016/j.biomaterials.2018.03.040
- Author:
- Jaewook KimThese authors contributed equally to this work.; In Kyong Shim; Dong Gyu Hwang; Yu Na Lee; Myungji Kim; Hyeonji Kim; Seok-Won Kim; Song Lee; Song Cheol Kim; Dong-Woo Cho; Jinah Jang
- Source:
- Journal of materials chemistry B 2019 v.7 no.10 pp. 1773-1781
- ISSN:
- 2050-7518
- Subject:
- bioprinting; chemistry; coculture; diabetes; endothelial cells; extracellular matrix; humans; hypoglycemia; insulin; insulin secretion; necrosis; pancreas; patients; stem cells; therapeutics; tissues
- Abstract:
- ... Type 1 diabetes mellitus (T1DM) is a form of diabetes that inhibits or halts insulin production in the pancreas. Although various therapeutic options are applied in clinical settings, not all patients are treatable with such methods due to the instability of the T1DM or the unawareness of hypoglycemia. Islet transplantation using a tissue engineering-based approach may mark a clinical significance ...
- DOI:
- 10.1039/c8tb02787k
- https://doi.org/10.1039/c8tb02787k
44. 3D cell-printing of biocompatible and functional meniscus constructs using meniscus‐derived bioink
- Author:
- Suhun Chae; Sung-Sahn Lee; Yeong-Jin Choi; Da Hee Hong; Ge Gao; Joon Ho Wang; Dong-Woo Cho
- Source:
- Biomaterials 2021 v.267 pp. 120466
- ISSN:
- 0142-9612
- Subject:
- anisotropy; biocompatibility; biocompatible materials; encapsulation; extracellular matrix; orthopedics; polyurethanes
- Abstract:
- ... Meniscus injuries are prevalent in orthopedic diagnosis. The reconstruction of the structural inhomogeneity and anisotropy of the meniscus is a major challenge in clinical practice. Meniscal tissue engineering has emerged as a potential alternative for the treatment of various meniscal diseases and injuries. In this study, we developed three-dimensional (3D) cell-printed meniscus constructs using ...
- DOI:
- 10.1016/j.biomaterials.2020.120466
- https://doi.org/10.1016/j.biomaterials.2020.120466
- Author:
- Joseph Candiello; Taraka Sai Pavan Grandhi; Saik Kia Goh; Vimal Vaidya; Maya Lemmon-Kishi; Kiarash Rahmani Eliato; Robert Ros; Prashant N. Kumta; Kaushal Rege; Ipsita Banerjee
- Source:
- Biomaterials 2018 v.177 pp. 27-39
- ISSN:
- 0142-9612
- Subject:
- c-peptide; embryonic stem cells; endothelial cells; extracellular matrix; genes; glucose; humans; hydrogels; islets of Langerhans; protein synthesis; reproduction
- Abstract:
- ... Organoids, which exhibit spontaneous organ specific organization, function, and multi-cellular complexity, are in essence the in vitro reproduction of specific in vivo organ systems. Recent work has demonstrated human pluripotent stem cells (hPSCs) as a viable regenerative cell source for tissue-specific organoid engineering. This is especially relevant for engineering islet organoids, due to the ...
- DOI:
- 10.1016/j.biomaterials.2018.05.031
- https://doi.org/10.1016/j.biomaterials.2018.05.031
- Author:
- Minseong Kim; GeunHyung Kim
- Source:
- Journal of colloid and interface science 2015 v.457 pp. 180-187
- ISSN:
- 0021-9797
- Subject:
- biobased products; biochemical pathways; cell adhesion; cellulose; cellulosic fibers; electric field; extracellular matrix; fibroblasts; geometry; humans; nanofibers; tissue engineering; topology
- Abstract:
- ... Micro/nanofibrous structures have been applied widely in various tissue-engineering applications because the topological structures are similar to the extracellular matrix (ECM), which encourages a high degree of cell adhesion and growth. However, it has been difficult to produce a three-dimensional (3D) fibrous structure using controllable macro-pores. Recently, cellulose has been considered a hi ...
- DOI:
- 10.1016/j.jcis.2015.07.007
- https://doi.org/10.1016/j.jcis.2015.07.007
- Author:
- Zhiyi Liu; Lucia Speroni; Kyle P. Quinn; Carlo Alonzo; Dimitra Pouli; Yang Zhang; Emily Stuntz; Carlos Sonnenschein; Ana M. Soto; Irene Georgakoudi
- Source:
- Biomaterials 2018 v.179 pp. 96-108
- ISSN:
- 0142-9612
- Subject:
- breasts; collagen; epithelium; estradiol; extracellular matrix; hormonal regulation; image analysis; models; morphogenesis; prolactin; tissue culture; variance
- Abstract:
- ... Hormones play an important role in normal and diseased breast tissue development. However, they can also disrupt cell-matrix interactions and their role in extracellular matrix reorganization during epithelial morphogenesis remains poorly understood, partly due to a lack of sensitive approaches for matrix characterization. Here, we assess the hormonal regulation of matrix reorganization in a three ...
- DOI:
- 10.1016/j.biomaterials.2018.06.036
- PubMed:
- 29980078
- PubMed Central:
- PMC6176712
- https://doi.org/10.1016/j.biomaterials.2018.06.036
- Author:
- Kailei Xu; Zi Wang; John A. Copland; Ratna Chakrabarti; Stephen J. Florczyk
- Source:
- Biomaterials 2020 v.254 pp. 120126
- ISSN:
- 0142-9612
- Subject:
- biocompatible materials; cadherins; chitosan; chondroitin sulfate; drug resistance; epithelium; extracellular matrix; metastasis; porosity; prognosis; prostatic neoplasms; sulfates; vimentin
- Abstract:
- ... Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens. Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa. Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications. Three-dimensional porous chitosan-CS (C-CS) scaf ...
- DOI:
- 10.1016/j.biomaterials.2020.120126
- https://doi.org/10.1016/j.biomaterials.2020.120126
- Author:
- Ning Xiang; John S.K. Yuen; Andrew J. Stout; Natalie R. Rubio; Ying Chen; David L. Kaplan
- Source:
- Biomaterials 2022 v.285 pp. 121543
- ISSN:
- 0142-9612
- Subject:
- biocompatible materials; cattle; crosslinking; cultured meat; extracellular matrix; glutenins; mice; myoblasts; plant source protein; refrigeration; toxicity; wheat
- Abstract:
- ... Scaffolds suitable for use in food products are crucial components for the production of cultured meat. Here, wheat glutenin, an inexpensive and abundant plant-based protein, was used to develop 3D porous scaffolds for cultured meat applications. A physical cross-linking method based on water annealing was developed for the fabrication of porous glutenin sponges and fibrous aligned scaffolds. The ...
- DOI:
- 10.1016/j.biomaterials.2022.121543
- https://doi.org/10.1016/j.biomaterials.2022.121543
- Author:
- Jinah Jang; Hun-Jun Park; Seok-Won Kim; Heejin Kim; Ju Young Park; Soo Jin Na; Hyeon Ji Kim; Moon Nyeo Park; Seung Hyun Choi; Sun Hwa Park; Sung Won Kim; Sang-Mo Kwon; Pum-Joon Kim; Dong-Woo Cho
- Source:
- Biomaterials 2017 v.112 pp. 264-274
- ISSN:
- 0142-9612
- Subject:
- extracellular matrix; fibrosis; heart diseases; hypertrophy; infarction; myocardium; stem cells; therapeutics; tissue repair
- Abstract:
- ... Stem cell therapy is a promising therapeutic method for the treatment of ischemic heart diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In t ...
- DOI:
- 10.1016/j.biomaterials.2016.10.026
- https://doi.org/10.1016/j.biomaterials.2016.10.026
- Author:
- Girdhari Rijal; Weimin Li
- Source:
- Biomaterials 2016 v.81 pp. 135-156
- ISSN:
- 0142-9612
- Subject:
- adipocytes; blood vessels; breast neoplasms; cell growth; drugs; extracellular matrix; metastasis; neoplasm cells; physical properties; stromal cells
- Abstract:
- ... Breast tumors grow in a tissue microenvironment containing extracellular matrix (ECM), adipocytes, stromal cells, fluids, and blood vessels. This natural yet complex physiopathological territory is dynamically remodeled in favor of tumor growth and metastasis. The environment-mimicking 3D cultures have shown compelling advantages in the studies of tumor cell biology, and are of intensive research ...
- DOI:
- 10.1016/j.biomaterials.2015.12.016
- https://doi.org/10.1016/j.biomaterials.2015.12.016
- Author:
- Marco Campisi; Yoojin Shin; Tatsuya Osaki; Cynthia Hajal; Valeria Chiono; Roger D. Kamm
- Source:
- Biomaterials 2018 v.180 pp. 117-129
- ISSN:
- 0142-9612
- Subject:
- astrocytes; blood-brain barrier; brain; clinical trials; confocal microscopy; drugs; endothelial cells; extracellular matrix; fibrin; gels; humans; immunocytochemistry; models; neurodegenerative diseases; pathogens; permeability; rats; tight junctions; transporters
- Abstract:
- ... The blood-brain barrier (BBB) regulates molecular trafficking, protects against pathogens, and prevents efficient drug delivery to the brain. Models to date failed to reproduce the human anatomical complexity of brain barriers, contributing to misleading results in clinical trials. To overcome these limitations, a novel 3-dimensional BBB microvascular network model was developed via vasculogenesis ...
- DOI:
- 10.1016/j.biomaterials.2018.07.014
- PubMed:
- 30032046
- PubMed Central:
- PMC6201194
- https://doi.org/10.1016/j.biomaterials.2018.07.014
- Author:
- Sofia P. Rebelo; Catarina Pinto; Tatiana R. Martins; Nathalie Harrer; Marta F. Estrada; Pablo Loza-Alvarez; José Cabeçadas; Paula M. Alves; Emilio J. Gualda; Wolfgang Sommergruber; Catarina Brito
- Source:
- Biomaterials 2018 v.163 pp. 185-197
- ISSN:
- 0142-9612
- Subject:
- alginates; cell lines; cell movement; chemokine CCL22; chemokine CCL24; chemokine CXCL1; coculture; collagen; disease course; drug therapy; drugs; extracellular matrix; fibroblasts; fibronectins; immunosuppression; immunotherapy; interleukin-10; interleukin-4; lung neoplasms; macrophages; metalloproteinases; microencapsulation; monocytes; neoplasm cells; phenotype; prediction
- Abstract:
- ... The tumour microenvironment (TME) shapes disease progression and influences therapeutic response. Most aggressive solid tumours have high levels of myeloid cell infiltration, namely tumour associated macrophages (TAM). Recapitulation of the interaction between the different cellular players of the TME, along with the extracellular matrix (ECM), is critical for understanding the mechanisms underlyi ...
- DOI:
- 10.1016/j.biomaterials.2018.02.030
- https://doi.org/10.1016/j.biomaterials.2018.02.030
- Author:
- Zhen Yang; Tianyuan Zhao; Cangjian Gao; Fuyang Cao; Hao Li; Zhiyao Liao; Liwei Fu; Pinxue Li; Wei Chen; Zhiqiang Sun; Shuangpeng Jiang; Zhuang Tian; Guangzhao Tian; Kangkang Zha; Tingting Pan; Xu Li; Xiang Sui; Zhiguo Yuan; Shuyun Liu; Quanyi Guo
- Source:
- ACS applied materials & interfaces 2021 v.13 no.20 pp. 23369-23383
- ISSN:
- 1944-8252
- Subject:
- biodegradability; bioprinting; cartilage; cell adhesion; chondrogenesis; extracellular matrix; gelatin; oligonucleotides; polymers; rabbits; strength (mechanics); therapeutics
- Abstract:
- ... Articular cartilage (AC) lesions are fairly common but remain an obstacle for clinicians and researchers due to their poor self-healing capacity. Recently, a promising therapy based on the recruitment of autologous mesenchymal stem cells (MSCs) has been developed for the regeneration of full-thickness cartilage defects in the knee joint. In this study, a 3D-bioprinted difunctional scaffold was dev ...
- DOI:
- 10.1021/acsami.1c01844
- https://doi.org/10.1021/acsami.1c01844
- Author:
- Yanzhi Liu; Liuqi Peng; Lingli Li; Cuishan Huang; Keda Shi; Xiangbo Meng; Pinpin Wang; Mingming Wu; Ling Li; Huijuan Cao; Kefeng Wu; Qingqiang Zeng; Haobo Pan; William Weijia Lu; Ling Qin; Changshun Ruan; Xinluan Wang
- Source:
- Biomaterials 2021 v.279 pp. 121216
- ISSN:
- 0142-9612
- Subject:
- animal models; biocompatible materials; biomimetics; cartilage; chondrogenesis; collagen; diclofenac; extracellular matrix; gait; hyaluronic acid; inflammation; mesenchymal stromal cells; osteoarthritis
- Abstract:
- ... Osteochondral defect repair in osteoarthritis (OA) remains an unsolved clinical problem due to the lack of enough seed cells in the defect and chronic inflammation in the joint. To address this clinical need, we designed a bone marrow-derived mesenchymal stem cell (BMSC)-laden 3D-bioprinted multilayer scaffold with methacrylated hyaluronic acid (MeHA)/polycaprolactone incorporating kartogenin and ...
- DOI:
- 10.1016/j.biomaterials.2021.121216
- https://doi.org/10.1016/j.biomaterials.2021.121216
- Author:
- Ashley L. Farris; Dennis Lambrechts; Yuxiao Zhou; Nicholas Y. Zhang; Naboneeta Sarkar; Megan C. Moorer; Alexandra N. Rindone; Ethan L. Nyberg; Alexander Perdomo-Pantoja; S.J. Burris; Kendall Free; Timothy F. Witham; Ryan C. Riddle; Warren L. Grayson
- Source:
- Biomaterials 2022 v.280 pp. 121318
- ISSN:
- 0142-9612
- Subject:
- antioxidants; biocompatible materials; biodegradability; bone formation; bones; extracellular matrix; humans; hyperoxia; hypoxia; mice; osteopontin; oxygen; polyvinyl alcohol; therapeutics; tissue repair; toxicity
- Abstract:
- ... Low oxygen (O₂) diffusion into large tissue engineered scaffolds hinders the therapeutic efficacy of transplanted cells. To overcome this, we previously studied hollow, hyperbarically-loaded microtanks (μtanks) to serve as O₂ reservoirs. To adapt these for bone regeneration, we fabricated biodegradable μtanks from polyvinyl alcohol and poly (lactic-co-glycolic acid) and embedded them to form 3D-pr ...
- DOI:
- 10.1016/j.biomaterials.2021.121318
- https://doi.org/10.1016/j.biomaterials.2021.121318
- Author:
- Raphaël F. Canadas; João B. Costa; Zhengwei Mao; Changyou Gao; Utkan Demirci; Rui L. Reis; Alexandra P. Marques; Joaquim M. Oliveira
- Source:
- Biomaterials 2021 v.277 pp. 121112
- ISSN:
- 0142-9612
- Subject:
- anisotropy; biocompatible materials; biomarkers; biomechanics; coculture; cryogels; drugs; extracellular matrix; ice; isotropy; liver cirrhosis; medicine; osteopontin
- Abstract:
- ... Natural extracellular matrix governs cells providing biomechanical and biofunctional outstanding properties, despite being porous and mostly made of soft materials. Among organs, specific tissues present specialized macro-architectures. For instance, hepatic lobules present radial organization, while vascular sinusoids are branched from vertical veins, providing specific biofunctional features. Th ...
- DOI:
- 10.1016/j.biomaterials.2021.121112
- https://doi.org/10.1016/j.biomaterials.2021.121112
- Author:
- García-Vilas Javier A.; Quesada Ana R.; Medina Miguel Ángel
- Source:
- Journal of agricultural and food chemistry 2013 v.61 no.17 pp. 4063-4071
- ISSN:
- 1520-5118
- Subject:
- adhesion; angiogenesis; biosynthesis; cell proliferation; endothelial cells; extracellular matrix; gelatinase A; gene expression regulation; hyaluronic acid; inhibitory concentration 50; minimum inhibitory concentration
- Abstract:
- ... 4-Methylumbelliferone (4-MU) is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects. The objective of the present study was to determine the potential of 4-MU as an antiangiogenic compound. To fulfill this aim, cultured endothelial cells were used to perform an array of in vitro assays, as well as two different in vivo angiogenesis assays. This study demonstrates t ...
- DOI:
- 10.1021/jf303062h
- PubMed:
- 23581646
- https://doi.org/10.1021/jf303062h
- Author:
- Karoly Jakab; Francoise Marga; Cyrille Norotte; Gabor Forgacs
- Source:
- Cryobiology 2015 v.71 no.1 pp. 165-166
- ISSN:
- 0011-2240
- Subject:
- bioprinting; bioreactors; extracellular matrix; longevity; morphogenesis; patients; tissue banks; tissue engineering; tissues
- Abstract:
- ... With aging population, increase in longevity and decrease in the number of qualified donors, the need to find alternative solutions to current organ replacement methods is rapidly becoming a critical issue. Tissue engineering has appeared as a potentially viable solution. Classical tissue engineering is based on culturing cells in scaffolds, artificial extracellular matrix mimics, with the hope th ...
- DOI:
- 10.1016/j.cryobiol.2015.05.010
- https://doi.org/10.1016/j.cryobiol.2015.05.010
- Author:
- Jolanta Opiela; Daniel Lipiński; Joanna Romanek; Wojciech Juzwa; Michał Bochenek; Piotr Wilczek
- Source:
- Annals of animal science 2016 v.16 no.2 pp. 369-385
- ISSN:
- 2300-8733
- Subject:
- adipocytes; adipogenesis; bone formation; extracellular matrix; flow cytometry; fluorescent antibody technique; gelatinase A; osteocalcin; polymerase chain reaction; stem cells; swine; transcription (genetics); transcription factors
- Abstract:
- ... Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) and its inhibitor TIMP-2, MEF2a and TAZ transcription factors are involved in porcine MSC differentiation towards adipocytes and osteocytes. Flow cytometry and immunofluorescence were used to investigate ...
- DOI:
- 10.1515/aoas-2015-0065
- https://doi.org/10.1515/aoas-2015-0065
- Author:
- M. Ghiringhelli; N. Verdile; T. A. L. Brevini; F. Gandolfi
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 151-152
- ISSN:
- 1031-3613
- Subject:
- DNA; analysis of variance; animal models; bioengineering; collagen; deoxycholic acid; dimethyl sulfoxide; extracellular matrix; female fertility; fluorescent antibody technique; goats; human population; medicine; opacity; patients; quantitative polymerase chain reaction; slaughter; uterus; veterinary medicine
- Abstract:
- ... Female infertility or absence of a functional uterus has a prevalence of 3 to 5% in the general human population. Nowadays most patients diagnosed with absolute uterine factor infertility remain untreatable. Moreover, the world population is aging rapidly, and as people live longer, uterus frailty increases the risk of developing age-related diseases. Whole-organ decellularization and recellulariz ...
- DOI:
- 10.1071/RDv31n1Ab52
- https://doi.org/10.1071/RDv31n1Ab52
- Author:
- Reggiani V. Gonçalves; Rômulo D. Novaes; Mariáurea M. Sarandy; Eduardo M. Damasceno; Sérgio L.P. da Matta; Neire M. de Gouveia; Mariella B. Freitas; Foued S. Espindola
- Source:
- Life sciences 2016 v.152 pp. 67-75
- ISSN:
- 0024-3205
- Subject:
- animal disease models; antioxidants; collagen; diabetes; ethics; extracellular matrix; intraperitoneal injection; laboratory animals; lanolin; ointments; rats; silver; sodium chloride; streptozotocin; sulfadiazine; superoxide dismutase; testosterone; tissue repair; tissues
- Abstract:
- ... Wound healing involves a complex interaction between the cells, extracellular matrix and oxidative response.Analyze the effects of 5α-Dihydrotestosterone (5α-DTH) ointment in cutaneous wound healing by secondary intention in diabetic Wistar rats.Rats (302.23±26.23g, n=48) were maintained in cages with food and water ad libitum in accordance with the Guiding Principles in the Use of Animal Ethics C ...
- DOI:
- 10.1016/j.lfs.2016.03.019
- https://doi.org/10.1016/j.lfs.2016.03.019
- Author:
- Zeeshan Syedain; Jay Reimer; Jillian Schmidt; Matthew Lahti; James Berry; Richard Bianco; Robert T. Tranquillo
- Source:
- Biomaterials 2015 v.73 pp. 175-184
- ISSN:
- 0142-9612
- Subject:
- DNA; animal models; calcification; collagen; compliance; elastin; extracellular matrix; hemodynamics; histology; in vitro studies; mechanical properties; patients; prostheses; sheep; surgery; tissue engineering
- Abstract:
- ... Diseased aortic valves often require replacement, with over 30% of the current aortic valve surgeries performed in patients who will outlive a bioprosthetic valve. While many promising tissue-engineered valves have been created in the lab using the cell-seeded polymeric scaffold paradigm, none have been successfully tested long-term in the aortic position of a pre-clinical model. The high pressure ...
- DOI:
- 10.1016/j.biomaterials.2015.09.016
- PubMed:
- 26409002
- PubMed Central:
- PMC5520964
- https://doi.org/10.1016/j.biomaterials.2015.09.016
- Author:
- H. Pérez Campos; M. Saldias; G. Sanchez; P. Martucci; M. Acosta; R. Faccio; L. Suescun; M. Romero; A. Mombru
- Source:
- Cryobiology 2012 v.65 no.3 pp. 340-341
- ISSN:
- 0011-2240
- Subject:
- Raman spectroscopy; X-radiation; X-ray diffraction; amnion; aseptic conditions; banking; biopolymers; chemical residues; collagen; cryopreservation; extracellular matrix; freezing; humans; placenta; pregnancy; public health; sodium chloride; temperature; tissues; Uruguay
- Abstract:
- ... INTRODUCTION: Preservation techniques are of fundamental support for conservation of tissue banking (TB) biological materials. However, controversial views remain on the effects at the molecular level that preservation procedures could produce on the functional structures of tissues. Both programmed cryopreservation and glycerolized tissue preservation are common TB methods in technical preservati ...
- DOI:
- 10.1016/j.cryobiol.2012.07.005
- https://doi.org/10.1016/j.cryobiol.2012.07.005
- Author:
- Tara D. Callaway; Anu Singh-Cundy
- Source:
- Plants 2019 v.8 no.7 pp. -
- ISSN:
- 2223-7747
- Subject:
- Nicotiana; Petunia axillaris; Petunia integrifolia; Solanum; environmental factors; extracellular matrix; genes; histidine; pistil; pollen tubes; pollinators; proteins; seed set; sympatric speciation
- Abstract:
- ... Transmitting tissue-specific proteins (TTS proteins) are abundant in the extracellular matrix of Nicotiana pistils, and vital for optimal pollen tube growth and seed set. We have identified orthologs from several species in the Solanaceae, including Petunia axillaris axillaris and Petunia integrifolia. We refer to TTS proteins and their orthologs as histidine domain-arabinogalactan proteins (HD-AG ...
- DOI:
- 10.3390/plants8070211
- PubMed:
- 31288469
- PubMed Central:
- PMC6681252
- https://doi.org/10.3390/plants8070211
- Author:
- Ha-Rim Kim; Seon-Young Kim
- Source:
- Nutrients 2019 v.11 no.2 pp. -
- ISSN:
- 2072-6643
- Subject:
- NAD(P)H oxidase (H2O2-forming); Perilla frutescens; cell proliferation; collagen; diabetic nephropathy; extracellular matrix; fibronectins; fibrosis; glucose; hyperglycemia; hypertrophy; mice; reactive oxygen species
- Abstract:
- ... Perilla frutescens (L.) Britt. var. japonica (Hassk.) Hara (PF), is a medical herb of the Lamiaceae family. We have previously reported that the PF sprout extract (PFSE) is effective in treating hyperglycemia. However, the role of PFSE on glomerular mesangial cells (MCs) proliferation and the extracellular matrix (ECM) accumulation in a diabetic condition are still unclear. Therefore, in this stud ...
- DOI:
- 10.3390/nu11020356
- PubMed:
- 30744045
- PubMed Central:
- PMC6413074
- https://doi.org/10.3390/nu11020356
- Author:
- Jonathan J. Campbell; Laur-Alexandru Botos; Timothy J. Sargeant; Natalia Davidenko; Ruth E. Cameron; Christine J. Watson
- Source:
- Integrative biology 2014 v.6 no.6 pp. 618-626
- ISSN:
- 1757-9708
- Subject:
- adipose tissue; autophagy; breast neoplasms; breasts; cell lines; coculture; collagen; cytokines; drugs; epithelial cells; epithelium; extracellular matrix; high-throughput screening methods; hyaluronic acid; macrophages; mammary glands; models; monocytes; phenotype; progeny; prolactin; signal transduction; therapeutics; tissue repair; weaning
- Abstract:
- ... Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring. These processes are mediated in part through Jak/Stat signalling pathways, which can be deregulated in breast cancer. Synthetic in vitro analogues of the breast cou ...
- DOI:
- 10.1039/c3ib40257f
- https://doi.org/10.1039/c3ib40257f
- Author:
- Hernández, Orville; Almeida, Agostinho J.; Gonzalez, Angel; Garcia, Ana Maria; Tamayo, Diana; Cano, Luz Elena; Restrepo, Angela; McEwen, Juan G.
- Source:
- Infection and immunity 2010 v.78 no.12 pp. 5280-5286
- ISSN:
- 0019-9567
- Subject:
- Paracoccidioides brasiliensis; animal models; antisense RNA; epithelial cells; extracellular matrix; gene expression; host-pathogen relationships; humans; hydrolases; immune response; pathogens; phylogeny; proteins; sequence analysis; viability; virulence; yeasts
- Abstract:
- ... One of the most crucial events during infection with the dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in the establishment of disease. In this study, we have evaluated the relevance of a 32-kDa protein, a putative adhesion member of the haloacid dehalogenase (HAD) superfamily of hydrolases, in the virulence of this fungus. Protein sequenc ...
- DOI:
- 10.1128/IAI.00692-10
- PubMed:
- 20876288
- PubMed Central:
- PMC2981316
- https://doi.org/10.1128/IAI.00692-10
- Author:
- Stella Totti; Mark C. Allenby; Susana Brito Dos Santos; Athanasios Mantalaris; Eirini G. Velliou
- Source:
- RSC advances 2018 v.8 no.37 pp. 20928-20940
- ISSN:
- 2046-2069
- Subject:
- adenocarcinoma; apoptosis; biomarkers; drugs; extracellular matrix; fibronectins; hypoxia-inducible factor 1; image analysis; in vivo studies; neoplasm cells; niches; pancreatic neoplasms; polyurethanes; prognosis; screening; survival rate; tissue engineering
- Abstract:
- ... Pancreatic ductal adenocarcinoma is an aggressive disease with an extremely low survival rate. This is due to the (i) poor prognosis and (ii) high resistance of the disease to current treatment options. The latter is partly due to the very complex and dense tissue/tumour microenvironment of pancreatic cancer, which contributes to the disease's progression and the inhibition of apoptotic pathways. ...
- DOI:
- 10.1039/c8ra02633e
- https://doi.org/10.1039/c8ra02633e
- Author:
- Yeong-Jin Choi; Young-Joon Jun; Dong Yeon Kim; Hee-Gyeong Yi; Su-Hun Chae; Junsu Kang; Juyong Lee; Ge Gao; Jeong-Sik Kong; Jinah Jang; Wan Kyun Chung; Jong-Won Rhie; Dong-Woo Cho
- Source:
- Biomaterials 2019 v.206 pp. 160-169
- ISSN:
- 0142-9612
- Subject:
- animal models; biomimetics; bioprinting; cell viability; extracellular matrix; hypoxia; innervation; muscles
- Abstract:
- ... Volumetric muscle loss (VML) is an irrecoverable injury associated with muscle loss greater than 20%. Although hydrogel-based 3D engineered muscles and the decellularized extracellular matrix (dECM) have been considered for VML treatment, they have shown limited efficacy. We established a novel VML treatment with dECM bioink using 3D cell printing technology. Volumetric muscle constructs composed ...
- DOI:
- 10.1016/j.biomaterials.2019.03.036
- https://doi.org/10.1016/j.biomaterials.2019.03.036
- Author:
- David T. Dudley; Xiao-Yan Li; Casey Y. Hu; Celina G. Kleer; Amanda L. Willis; Stephen J. Weiss
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.41 pp. 14882-14887
- ISSN:
- 0027-8424
- Subject:
- antigens; antineoplastic activity; breast neoplasms; carcinoma; cell growth; collagen; humans; integrins; monoclonal antibodies; neoplasm cells; patients; tissues
- Abstract:
- ... Efforts to develop unbiased screens for identifying novel function-blocking monoclonal antibodies (mAbs) in human carcinomatous states have been hampered by the limited ability to design in vitro models that recapitulate tumor cell behavior in vivo. Given that only invasive carcinoma cells gain permanent access to type I collagen-rich interstitial tissues, an experimental platform was established ...
- DOI:
- 10.1073/pnas.1410996111
- PubMed:
- 25267635
- PubMed Central:
- PMC4205646
- https://doi.org/10.1073/pnas.1410996111
72. A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate®
- Author:
- Kristin M. Bircsak; Richard DeBiasio; Mark Miedel; Alaa Alsebahi; Ryan Reddinger; Anthony Saleh; Tongying Shun; Lawrence A. Vernetti; Albert Gough
- Source:
- Toxicology 2021 v.450 pp. 152667
- ISSN:
- 0300-483X
- Subject:
- albumins; automation; cell viability; coculture; dosage; dose response; endothelial cells; extracellular matrix; hepatocytes; hepatotoxicity; high-throughput screening methods; laboratories; liquids; liver; macrophages; models; organ-on-a-chip; prioritization; protocols; screening; secretion; staining; toxicology; urea; viability assays
- Abstract:
- ... We report the development, automation and validation of a 3D, microfluidic liver-on-a-chip for high throughput hepatotoxicity screening, the OrganoPlate LiverTox™. The model is comprised of aggregates of induced pluripotent stem cell (iPSC)-derived hepatocytes (iHep) seeded in an extracellular matrix in the organ channel and co-cultured with endothelial cells and THP-1 monoblasts differentiated to ...
- DOI:
- 10.1016/j.tox.2020.152667
- https://doi.org/10.1016/j.tox.2020.152667
73. A 3D tension bioreactor platform to study the interplay between ECM stiffness and tumor phenotype
- Author:
- Luke Cassereau; Yekaterina A. Miroshnikova; Guanqing Ou; Johnathon Lakins; Valerie M. Weaver
- Source:
- Journal of biotechnology 2015 v.193 pp. 66-69
- ISSN:
- 0168-1656
- Subject:
- mechanics; topography; phenotype; cardiovascular diseases; neoplasms; porosity; bioreactors; epithelial cells; hydrocolloids; collagen; neoplasm cells; mammary glands; hyaluronic acid; extracellular matrix
- Abstract:
- ... Extracellular matrix (ECM) structure, composition, and stiffness have profound effects on tissue development and pathologies such as cardiovascular disease and cancer. Accordingly, a variety of synthetic hydrogel systems have been designed to study the impact of ECM composition, density, mechanics, and topography on cell and tissue phenotype. However, these synthetic systems fail to accurately rec ...
- DOI:
- 10.1016/j.jbiotec.2014.11.008
- PubMed:
- 25435379
- PubMed Central:
- PMC4454500
- https://doi.org/10.1016/j.jbiotec.2014.11.008
74. A 3D tissue model-on-a-chip for studying the effects of human senescent fibroblasts on blood vessels
- Author:
- Joris Pauty; Shizuka Nakano; Ryo Usuba; Tadaaki Nakajima; Yoshikazu Johmura; Satotaka Omori; Naoya Sakamoto; Akihiko Kikuchi; Makoto Nakanishi; Yukiko T. Matsunaga
- Source:
- Biomaterials science 2021 v.9 no.1 pp. 199-211
- ISSN:
- 2047-4849
- Subject:
- angiogenesis; biocompatible materials; blood; cell senescence; collagen; extracellular matrix; fibroblasts; gels; humans; longevity; microvessels; models; phenotype
- Abstract:
- ... All human tissues experience aging that eventually causes organ dysfunction and disease. Cellular senescence was discovered in fibroblasts cultured in vitro. In adults, it is a primary defense mechanism against cancer, but also a major contributor to lifespan limits and disorders associated with aging. To assess how human blood vessels change in an aged environment, we developed an elementary tiss ...
- DOI:
- 10.1039/d0bm01297a
- https://doi.org/10.1039/d0bm01297a
75. A Biomimetic Macroporous Hybrid Scaffold with Sustained Drug Delivery for Enhanced Bone Regeneration
- Author:
- Seunghun S. Lee; Matthias Santschi; Stephen J. Ferguson
- Source:
- Biomacromolecules 2021 v.22 no.6 pp. 2460-2471
- ISSN:
- 1526-4602
- Subject:
- alizarin; apatite; biocompatibility; biomimetics; body fluids; bone formation; bone morphogenetic proteins; cell proliferation; cryogels; drugs; extracellular matrix; gelatin; heparin; hydrophilicity; hydrophobicity; porous media; quantitative polymerase chain reaction; strength (mechanics)
- Abstract:
- ... Bone regeneration is a highly complex physiological process regulated by several factors. In particular, bone-mimicking extracellular matrix and available osteogenic growth factors such as bone morphogenetic protein (BMP) have been regarded as key contributors for bone regeneration. In this study, we developed a biomimetic hybrid scaffold (CEGH) with sustained release of BMP-2 that would result in ...
- DOI:
- 10.1021/acs.biomac.1c00241
- https://doi.org/10.1021/acs.biomac.1c00241
- Author:
- Gimenez-Bastida, Juan A.; Martinez-Florensa, Mario; Espin, Juan-Carlos; Tomas-Barberan, Francisco A.; Garcia-Conesa, Maria-Teresa
- Source:
- Journal of agricultural and food chemistry 2009 v.57 no.19 pp. 9305-9315
- ISSN:
- 0021-8561
- Subject:
- anti-inflammatory activity; metalloproteinases; plant extracts; cell adhesion; extracellular matrix; flavanones; tissue repair; fibrosis; gene expression; gene expression regulation; colon; transcription factors; human cell lines; messenger RNA; tumor necrosis factor-alpha; in vitro studies; monocytes; inflammation; oranges; plasminogen activator; fibroblasts
- Abstract:
- ... The consumption of flavonoid-rich Citrus extracts has been associated with multiple beneficial effects including anti-inflammatory properties, but the potential effects on the inflammatory responses in the gut have not been thoroughly investigated. We used microarrays to search for molecular changes induced in human colon fibroblasts in response to the exposure to a flavanone-rich bitter orange ex ...
- DOI:
- 10.1021/jf901983g
- PubMed:
- 19728713
- https://doi.org/10.1021/jf901983g
- Author:
- Jinyuan Yan; Anthony G. Garza; Michael D. Bradley; Roy D. Welch
- Source:
- Journal of bacteriology 2012 v.194 no.7 pp. 1689-1696
- ISSN:
- 0021-9193
- Subject:
- Myxococcus xanthus; bacteria; cell movement; extracellular matrix; genes; loci; molecular chaperones; phenotype; pleiotropy; protein degradation; sporulation
- Abstract:
- ... The Clp/Hsp100 proteins are chaperones that play a role in protein degradation and reactivation. In bacteria, they exhibit a high degree of pleiotropy, affecting both individual and multicellular phenotypes. In this article, we present the first characterization of a Clp/Hsp100 homolog in Myxococcus xanthus (MXAN_4832 gene locus). Deletion of MXAN_4832 causes defects in both swarming and aggregati ...
- DOI:
- 10.1128/JB.06492-11
- PubMed:
- 22287524
- PubMed Central:
- PMC3302451
- https://doi.org/10.1128/JB.06492-11
- Author:
- Moshe Shemesh; Yunrong Chai
- Source:
- Journal of bacteriology 2013 v.195 no.12 pp. 2747-2754
- ISSN:
- 0021-9193
- Subject:
- Bacillus cereus; Bacillus subtilis; biofilm; extracellular matrix; glycerol; histidine kinase; manganese; operon; phosphorylation; spore-forming bacteria; sporulation; transcription (genetics); transcription factors
- Abstract:
- ... The spore-forming bacterium Bacillus subtilis forms matrix-enclosed biofilms in response to environmental cues that to date remain poorly defined. Biofilm formation depends on the synthesis of an extracellular matrix, which is indirectly regulated by the transcriptional regulator Spo0A. The activity of Spo0A depends on its phosphorylation state. The level of phosphorylated Spo0A (Spo0A∼P) is contr ...
- DOI:
- 10.1128/JB.00028-13
- PubMed:
- 23564171
- PubMed Central:
- PMC3697245
- https://doi.org/10.1128/JB.00028-13
- Author:
- Beste Kinikoglu
- Source:
- ATLA. Alternatives to laboratory animals v.45 no.6 pp. 309-316
- ISSN:
- 0261-1929
- Subject:
- artificial skin; basement membrane; collagen; cosmetics; extracellular matrix; fibroblasts; immunohistochemistry; laboratories; models; polymers
- Abstract:
- ... Tissue engineered full-thickness human skin substitutes have various applications in the clinic and in the laboratory, such as in the treatment of burns or deep skin defects, and as reconstructed human skin models in the safety testing of drugs and cosmetics and in the fundamental study of skin biology and pathology. So far, different approaches have been proposed for the generation of reconstruct ...
- DOI:
- 10.1177/026119291704500607
- https://doi.org/10.1177/026119291704500607
- Author:
- Hooker, Tanya S.; Lam, Patricia; Zheng, Huanquan; Kunst, Ljerka
- Source:
- plant cell 2007 v.19 no.3 pp. 904-913
- ISSN:
- 1040-4651
- Subject:
- Arabidopsis thaliana; biosynthesis; cutin; epicuticular wax; extracellular matrix; genes; messenger RNA; plant organs; polyesters; proteins; ribonucleases; yeasts
- Abstract:
- ... The cuticle is an extracellular matrix composed of cutin polyester and waxes that covers aerial organs of land plants and protects them from environmental stresses. The Arabidopsis thaliana cer7 mutant exhibits reduced cuticular wax accumulation and contains considerably lower transcript levels of ECERIFERUM3/WAX2/YORE-YORE (CER3/WAX2/YRE), a key wax biosynthetic gene. We show here that CER7 prote ...
- DOI:
- 10.1105/tpc.106.049304
- PubMed:
- 17351114
- PubMed Central:
- PMC1867365
- https://doi.org/10.1105/tpc.106.049304
- Author:
- Wenzhen Zhu; Jabed Iqbal; Dong-An Wang
- Source:
- Journal of materials chemistry B 2019 v.7 no.10 pp. 1741-1752
- ISSN:
- 2050-7518
- Subject:
- animals; bioadhesives; biocompatibility; cartilage; chondroitin sulfate; dietary supplements; extracellular matrix; hydrogels; pharmaceutical industry; tissue repair; water content
- Abstract:
- ... Great progress has been achieved on the study of hydrogels, which were presented for the first time in 1960 by Otto Wichterle and Drahoslav Lím. The two crucial properties of hydrogels, namely high water content and biocompatibility, have made hydrogels ideal compositions in the development of bioadhesives in recent years. Chondroitin sulfate (CS), a sulfated glycosaminoglycan (GAG), is distribute ...
- DOI:
- 10.1039/c8tb01990h
- https://doi.org/10.1039/c8tb01990h
- Author:
- Mizuho Kunii; Mami Yasuno; Yuki Shindo; Takefumi Kawata
- Source:
- Development genes and evolution 2014 v.224 no.1 pp. 25-35
- ISSN:
- 0949-944X
- Subject:
- Dictyostelium discoideum; cellulose; cellulose 1,4-beta-cellobiosidase; culture media; endo-1,4-beta-glucanase; extracellular matrix; fruiting bodies; gel electrophoresis; genes; mutants; spores
- Abstract:
- ... Dictyostelium discoideum is a facultative multicellular amoebozoan with cellulose in the stalk and spore coat of its fruiting body as well as in the extracellular matrix of the migrating slug. The organism also harbors a number of cellulase genes. One of them, cbhA, was identified as a candidate cellobiohydrolase gene based on the strong homology of its predicted protein product to fungal cellobio ...
- DOI:
- 10.1007/s00427-013-0460-x
- PubMed:
- 24240571
- https://doi.org/10.1007/s00427-013-0460-x
- Author:
- Kenneth C. Koehler; Daniel L. Alge; Kristi S. Anseth; Christopher N. Bowman
- Source:
- Biomaterials 2013 v.34 no.16 pp. 4150-4158
- ISSN:
- 0142-9612
- Subject:
- alkaline phosphatase; bone formation; dexamethasone; ethylene glycol; extracellular matrix; humans; hydrocolloids; mineralization; models; molecular weight; stem cells
- Abstract:
- ... We report a new approach to controlled drug release based upon exploiting the dynamic equilibrium that exists between Diels–Alder reactants and products, demonstrating the release of a furan containing dexamethasone peptide (dex-KGPQG-furan) from a maleimide containing hydrogel. Using a reaction-diffusion model, the release kinetics were tuned to achieve sustained concentrations conducive to osteo ...
- DOI:
- 10.1016/j.biomaterials.2013.02.020
- PubMed:
- 23465826
- PubMed Central:
- PMC3604741
- https://doi.org/10.1016/j.biomaterials.2013.02.020
- Author:
- Brandon W. Peterson; Henny C. van der Mei; Jelmer Sjollema; Henk J. Busscher; Prashant K. Sharma
- Source:
- mBio 2013 v.4 no.5 pp. e00497-13
- ISSN:
- 2150-7511
- Subject:
- DNA; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; Streptococcus mutans; bacteria; biofilm; chemical composition; deformation; extracellular matrix; models; polysaccharides; proteins; stress relaxation; viscoelasticity
- Abstract:
- ... Bacteria in the biofilm mode of growth are protected against chemical and mechanical stresses. Biofilms are composed, for the most part, of extracellular polymeric substances (EPSs). The extracellular matrix is composed of different chemical constituents, such as proteins, polysaccharides, and extracellular DNA (eDNA). Here we aimed to identify the roles of different matrix constituents in the vis ...
- DOI:
- 10.1128/mBio.00497-13
- PubMed:
- 24129256
- PubMed Central:
- PMC3812712
- https://doi.org/10.1128/mBio.00497-13
- Author:
- Rui Wang; Xuexue Wang; Yijing Zhan; Zheng Xu; Zongqi Xu; Xiaohai Feng; Sha Li; Hong Xu
- Source:
- ACS applied materials & interfaces 2019 v.11 no.41 pp. 37502-37512
- ISSN:
- 1944-8252
- Subject:
- UV filters; bioadhesives; extracellular matrix; hydrogels; hydrogen bonding; microstructure; polyglutamic acid; sunscreens; tannins
- Abstract:
- ... Novel sunscreen products based on bioadhesive/gel systems that can prevent the skin penetration behaviors of UV filters have attracted increasing attention in recent years. However, integration is very difficult to achieve and control on the wet surface of the skin under sweaty/dynamic physiological conditions, resulting in functional failure. Herein, we demonstrated the fabrication of a novel dua ...
- DOI:
- 10.1021/acsami.9b14538
- https://doi.org/10.1021/acsami.9b14538
- Author:
- Stiess Michael; Wegehingel Sabine; Nguyen Chuong; Nickel Walter; Bradke Frank; Cambridge Sidney B.
- Source:
- Journal of Proteome Research 2015 v.14 no.8 pp. 3229-3238
- ISSN:
- 1535-3907
- Subject:
- blood serum; cerebellum; neurons; protein secretion; proteins; proteome; proteomics; tissues
- Abstract:
- ... Recent evidence suggests that the extracellular protein milieu is much more complex than previously assumed as various secretome analyses from different cell types described the release of hundreds to thousands of proteins. The extracellular function of many of these proteins has yet to be determined particularly in the context of three-dimensional tissues with abundant cell–cell contacts. Toward ...
- DOI:
- 10.1021/acs.jproteome.5b00199
- https://doi.org/10.1021/acs.jproteome.5b00199
- Author:
- Fenghua Wang; Xiangyu Sha; Xiaolu Song; Mengqi Bai; Xiaohua Tian; Lei Liu
- Source:
- Langmuir 2021 v.37 no.50 pp. 14638-14645
- ISSN:
- 1520-5827
- Subject:
- adhesion; biocompatible materials; biomimetics; chemical bonding; enzymes; extracellular matrix; medicine; peptides; sugars
- Abstract:
- ... As mimics of the extracellular matrix, surfaces with the capability of capturing and releasing specific cells in a smart and controllable way play an important role in bacterial isolation. In this work, we fabricated a dual-responsive smart biointerface via peptide self-assembly and reversible covalent chemistry biomimetic adhesion behavior for bacterial isolation. Compared with that of the bioint ...
- DOI:
- 10.1021/acs.langmuir.1c02357
- https://doi.org/10.1021/acs.langmuir.1c02357
- Author:
- Chang Yuan; Liu Zheng; Zhang Yun; Galior Kornelia; Yang Jeffery; Salaita Khalid
- Source:
- Journal of the American Chemical Society 2016 v.138 no.9 pp. 2901-2904
- ISSN:
- 1520-5126
- Subject:
- extracellular matrix; fluorescence; fluorescent dyes; image analysis; integrins; ligands; mechanotransduction; receptors; temporal variation
- Abstract:
- ... Mechanical forces between cells and their extracellular matrix (ECM) are mediated by dozens of different receptors. These biophysical interactions play fundamental roles in processes ranging from cellular development to tumor progression. However, mapping the spatial and temporal dynamics of tension among various receptor–ligand pairs remains a significant challenge. To address this issue, we have ...
- DOI:
- 10.1021/jacs.5b11602
- PubMed:
- 26871302
- PubMed Central:
- PMC5589465
- https://doi.org/10.1021/jacs.5b11602
- Author:
- Madeline Y. Wong; Ngoc Duc Doan; Andrew S. DiChiara; Louis J. Papa; Jaime H. Cheah; Christian K. Soule; Nicki Watson; John D. Hulleman; Matthew D. Shoulders
- Source:
- Biochemistry 2018 v.57 no.19 pp. 2814-2827
- ISSN:
- 1520-4995
- Subject:
- biosynthesis; bone formation; cell lines; collagen; endoplasmic reticulum; extracellular matrix; fibrosis; genetic disorders; humans; luminescence; models; neoplasms; secretion; therapeutics; type I secretion system
- Abstract:
- ... Collagen overproduction is a feature of fibrosis and cancer, while insufficient deposition of functional collagen molecules and/or the secretion of malformed collagen is common in genetic disorders like osteogenesis imperfecta. Collagen secretion is an appealing therapeutic target in these and other diseases, as secretion directly connects intracellular biosynthesis to collagen deposition and biol ...
- DOI:
- 10.1021/acs.biochem.8b00378
- PubMed:
- 29676157
- PubMed Central:
- PMC6231715
- https://doi.org/10.1021/acs.biochem.8b00378
- Author:
- Anne-Kathrin Pöppel; Mareike Kahl; Andre Baumann; Jochen Wiesner; Anke Gökçen; Annika Beckert; Klaus T. Preissner; Andreas Vilcinskas; Zdeněk Franta
- Source:
- Insect biochemistry and molecular biology 2016 v.70 pp. 138-147
- ISSN:
- 0965-1748
- Subject:
- Drosophila melanogaster; Escherichia coli; Lucilia cuprina; Lucilia sericata; casein; chymotrypsin; coagulation; collagen; debridement; disinfection; drugs; excretion; extracellular matrix; fibronectins; humans; insect larvae; laminin; pH; temperature; tissue repair
- Abstract:
- ... Lucilia sericata larvae are used in maggot debridement therapy, a traditional wound healing approach that has recently been approved for the treatment of chronic wounds. Maggot excretion products (MEP) contain many different proteases that promote disinfection, debridement and the acceleration of wound healing, e.g. by activating the host contact phase/intrinsic pathway of coagulation. In order to ...
- DOI:
- 10.1016/j.ibmb.2015.11.012
- https://doi.org/10.1016/j.ibmb.2015.11.012
- Author:
- Luis P. B. Guerzoni; Yoshinari Tsukamoto; David B. Gehlen; Dirk Rommel; Tamás Haraszti; Mitsuru Akashi; Laura De Laporte
- Source:
- Biomacromolecules 2019 v.20 no.10 pp. 3746-3754
- ISSN:
- 1526-4602
- Subject:
- cardiomyocytes; cell transplantation; cell viability; coatings; coculture; embryonic stem cells; encapsulation; extracellular matrix; fibroblasts; induced pluripotent stem cells; infarction; microfluidic technology; microgels; peptides; polyethylene glycol; tissue repair
- Abstract:
- ... Human induced pluripotent stem cells (hiPSCs) are used as an alternative for human embryonic stem cells. Cardiomyocytes derived from hiPSCs are employed in cardiac tissue regeneration constructs due to the heart’s low regeneration capacity after infarction. A coculture of hiPSC-CM and primary dermal fibroblasts is encapsulated in injectable poly(ethylene glycol)-based microgels via microfluidics t ...
- DOI:
- 10.1021/acs.biomac.9b00786
- https://doi.org/10.1021/acs.biomac.9b00786
- Author:
- Karin Legerstee; Adriaan B. Houtsmuller
- Source:
- Biology 2021 v.10 no.11 pp. -
- ISSN:
- 2079-7737
- Subject:
- actin; cell movement; cytoskeleton; extracellular matrix; fluorescence; integrins; physiological transport; plasma membrane; talin
- Abstract:
- ... The cytoskeleton provides structure to cells and supports intracellular transport. Actin fibres are crucial to both functions. Focal Adhesions (FAs) are large macromolecular multiprotein assemblies at the ends of specialised actin fibres linking these to the extracellular matrix. FAs translate forces on actin fibres into forces contributing to cell migration. This review will discuss recent insigh ...
- DOI:
- 10.3390/biology10111189
- https://doi.org/10.3390/biology10111189
- Author:
- Hirofumi HIRAYASU; Yumiko YOSHIKAWA; Satoshi TSUZUKI; Tohru FUSHIKI
- Source:
- Bioscience, biotechnology, and biochemistry 2008 v.72 no.9 pp. 2294-2302
- ISSN:
- 1347-6947
- Subject:
- adhesion; apoptosis; biotechnology; cell lines; collagen; cytotoxicity; deformation; epithelial cells; extracellular matrix; fibronectins; laminin; rats; serine proteinases; small intestine
- Abstract:
- ... Granzyme A (GzmA) is a serine protease (trypsin-like specificity) produced in cytotoxic lymphocytes. This enzyme is believed to enter virus-infected cells and growing tumors and induce apoptosis, but the roles of GzmA expressed in lymphocytes scattered through the epithelial layer of the normal small intestine are unknown. In the present study, recombinant rat GzmA (rGzmA) was found to cause morph ...
- DOI:
- 10.1271/bbb.80140
- https://doi.org/10.1271/bbb.80140
- Author:
- Bernier, Steve P.; Nguyen, David T.; Sokol, Pamela A.
- Source:
- Infection and immunity 2008 v.76 no.1 pp. 38-47
- ISSN:
- 0019-9567
- Subject:
- Burkholderia cepacia complex; agar; alfalfa; biofilm; biomass; extracellular matrix; genes; histopathology; models; mutagenesis; mutants; open reading frames; phenotype; seedlings; transcription factors; transmission electron microscopy; transposons; virulence
- Abstract:
- ... Burkholderia cenocepacia strain K56-2 typically has rough colony morphology on agar medium; however, shiny colony variants (shv) can appear spontaneously. These shv all had a minimum of 50% reduction in biomass formation and were generally avirulent in an alfalfa seedling infection model. Three shv--K56-2 S15, K56-2 S76, and K56-2 S86--were analyzed for virulence in a chronic agar bead model of re ...
- DOI:
- 10.1128/IAI.00874-07
- PubMed:
- 17967860
- PubMed Central:
- PMC2223663
- https://doi.org/10.1128/IAI.00874-07
- Author:
- Rémi Cousin; Hugo Groult; Chanez Manseur; Romain Ferru-Clément; Mario Gani; Rachel Havret; Claire Toucheteau; Grégoire Prunier; Béatrice Colin; Franck Morel; Jean-Marie Piot; Isabelle Lanneluc; Kévin Baranger; Thierry Maugard; Ingrid Fruitier-Arnaudin
- Source:
- Marine drugs 2021 v.19 no.10 pp. -
- ISSN:
- 1660-3397
- Subject:
- breast neoplasms; cell movement; cell viability; cytotoxicity; enzyme activity; extracellular matrix; heparan sulfate; heparin; humans; metabolism; migratory behavior; oligosaccharides; pathogenesis
- Abstract:
- ... Sugar-based molecules such as heparins or natural heparan sulfate polysaccharides have been developed and widely studied for controlling heparanase (HPSE) enzymatic activity, a key player in extracellular matrix remodelling during cancer pathogenesis. However, non-enzymatic functions of HPSE have also been described in tumour mechanisms. Given their versatile properties, we hypothesized that sugar ...
- DOI:
- 10.3390/md19100546
- https://doi.org/10.3390/md19100546
- Author:
- Xiaoli Huang; Na Guan; Qiu Li
- Source:
- Marine drugs 2021 v.19 no.9 pp. -
- ISSN:
- 1660-3397
- Subject:
- angiogenesis; biocompatible materials; extracellular matrix; inflammation; polysaccharides; polyvinyl alcohol; tissue repair
- Abstract:
- ... Reconstructing the typical analogue of extracellular matrix (ECM) in engineered biomaterials is essential for promoting tissue repair. Here, we report an ECM-mimetic scaffold that successfully accelerated wound healing through enhancing vascularization and regulating inflammation. We prepared an electrospun fiber comprising a brown alga-derived polysaccharide (BAP) and polyvinyl alcohol (PVA). The ...
- DOI:
- 10.3390/md19090496
- https://doi.org/10.3390/md19090496
- Author:
- Mariaceleste Aragona; Tito Panciera; Andrea Manfrin; Stefano Giulitti; Federica Michielin; Nicola Elvassore; Sirio Dupont; Stefano Piccolo
- Source:
- Cell 2013 v.154 pp. 1047-1059
- ISSN:
- 0092-8674
- Subject:
- extracellular matrix; mechanical stress; proteins; tissues
- Abstract:
- ... Key cellular decisions, such as proliferation or growth arrest, typically occur at spatially defined locations within tissues. Loss of this spatial control is a hallmark of many diseases, including cancer. Yet, how these patterns are established is incompletely understood. Here, we report that physical and architectural features of a multicellular sheet inform cells about their proliferative capac ...
- DOI:
- 10.1016/j.cell.2013.07.042
- https://doi.org/10.1016/j.cell.2013.07.042
- Author:
- Yuree Lee; Maria C. Rubio; Julien Alassimone; Niko Geldner
- Source:
- Cell 2013 v.153 pp. 402-412
- ISSN:
- 0092-8674
- Subject:
- Arabidopsis; NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); cell wall components; cell walls; cells; endodermis; extracellular matrix; lignin; models; peroxidases; polymerization; proteins; reactive oxygen species
- Abstract:
- ... The precise localization of extracellular matrix and cell wall components is of critical importance for multicellular organisms. Lignin is a major cell wall modification that often forms intricate subcellular patterns that are central to cellular function. Yet the mechanisms of lignin polymerization and the subcellular precision of its formation remain enigmatic. Here, we show that the Casparian s ...
- DOI:
- 10.1016/j.cell.2013.02.045
- https://doi.org/10.1016/j.cell.2013.02.045
- Author:
- Roberta Besio; Maria Camilla Baratto; Roberta Gioia; Enrico Monzani; Stefania Nicolis; Lucia Cucca; Antonella Profumo; Luigi Casella; Riccardo Basosi; Ruggero Tenni; Antonio Rossi; Antonella Forlino
- Source:
- BBA - Proteins and Proteomics 2013 v.1834 pp. 197-204
- ISSN:
- 1570-9639
- Subject:
- Escherichia coli; active sites; carcinogenesis; catalytic activity; denaturation; extracellular matrix; fluorescence emission spectroscopy; genes; humans; hydrolysis; imino acids; manganese; mutation; proline dipeptidase; protein folding; protein metabolism; proteolysis
- Abstract:
- ... Human prolidase, the enzyme responsible for the hydrolysis of the Xaa-Pro/Hyp peptide bonds, is a key player in the recycling of imino acids during the final stage of protein catabolism and extracellular matrix remodeling. Its metal active site composition corresponding to the maximal catalytic activity is still unknown, although prolidase function is of increasing interest due to the link with ca ...
- DOI:
- 10.1016/j.bbapap.2012.09.008
- https://doi.org/10.1016/j.bbapap.2012.09.008
- Author:
- Blair, Kris M.; Turner, Linda; Winkelman, Jared T.; Berg, Howard C.; Kearns, Daniel B.
- Source:
- Science 2008 v.320 no.5883 pp. 1636-1638
- ISSN:
- 0036-8075
- Subject:
- Bacillus subtilis; bacteria; biofilm; biosynthesis; drug resistance; extracellular matrix; flagellum; operon
- Abstract:
- ... Biofilms are multicellular aggregates of sessile bacteria encased by an extracellular matrix and are important medically as a source of drug-resistant microbes. In Bacillus subtilis, we found that an operon required for biofilm matrix biosynthesis also encoded an inhibitor of motility, EpsE. EpsE arrested flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-genera ...
- DOI:
- 10.1126/science.1157877
- https://doi.org/10.1126/science.1157877