Subject term: extracellular matrix - PubAg Search Results

1. (+)-Terpinen-4-ol Inhibits Bacillus cereus Biofilm Formation by Upregulating the Interspecies Quorum Sensing Signals Diketopiperazines and Diffusing Signaling Factors

Author:: Lijun Zhao; Feixia Duan; Meng Gong; Xue Tian; Yan Guo; Lirong Jia; Sha Deng
Source:: Journal of agricultural and food chemistry 2021 v.69 no.11 pp. 3496-3510
ISSN:: 1520-5118
Subject:: Bacillus cereus; biofilm; diketopiperazines; enzyme activity; exopolysaccharides; extracellular matrix; food chemistry; food pathogens; gene expression; growth retardation; monoterpenoids; phenotype; spore germination
Abstract:: ... Bacillus cereus is a Gram-positive endospore-forming foodborne pathogen that causes lethal food poisoning and significant economic losses, usually through biofilm- and endospore-induced recurrent cross- and postprocessing contamination. Due to the lack of critical inhibitory targets and control strategies, B. cereus biofilm contamination is a problem that urgently needs a solution. In this study, ...
DOI:: 10.1021/acs.jafc.0c07826
: https://doi.org/10.1021/acs.jafc.0c07826

2. (±)-Aspongamide A, an N-Acetyldopamine Trimer Isolated from the Insect Aspongopus chinensis, Is an Inhibitor of p-Smad3

Author:: Yan Yong-Ming; Ai Jun; Shi Yan-Ni; Zuo Zhi-Li; Hou Bo; Luo Jie; Cheng Yong-Xian
Source:: Organic letters 2014 v.16 no.2 pp. 532-535
ISSN:: 1523-7052
Subject:: Aspongopus; enantiomers; extracellular matrix; insects; organic compounds; phosphorylation; rats; spectroscopy; transforming growth factor beta 1
Abstract:: ... (±)-Aspongamide A (1), an unusual trimer of N-acetyldopamine (NADA) bearing a novel tetrahydrobenzo[a]dibenzo[b,e][1,4]dioxine structure, and a pair of NADA dimeric enantiomers (2) were isolated from Aspongopus chinensis. The structures of compounds 1 and 2 were assigned using spectroscopic methods. Compound 1 was found to be an inhibitor of Smad3 phosphorylation in transforming growth factor-β1 ( ...
DOI:: 10.1021/ol403409v
: https://doi.org/10.1021/ol403409v

3. (−)-Epigallocatechin-3-gallate inhibits matrix metalloproteinases in oral ulcers

Author:: Bin Huang; Huangqin Chen
Source:: RSC advances 2015 v.5 no.30 pp. 23758-23766
ISSN:: 2046-2069
Subject:: Western blotting; acetic acid; animal models; colorimetry; dose response; enzyme-linked immunosorbent assay; epigallocatechin gallate; extracellular matrix; fluorometry; immunohistochemistry; interleukin-1beta; luciferase; macrophages; metalloproteinases; quantitative polymerase chain reaction; transcription (genetics)
Abstract:: ... The overexpression of matrix metalloproteinases (MMPs) results in excessive extracellular matrix degradation and oral ulcer healing delay. This study investigated the inhibitory effect of (−)-epigallocatechin-3-gallate (EGCG) on MMPs, the possible molecular mechanism and its implications for oral ulcers. MMP-1 and MMP-9 inhibition was detected using fluorometric and colorimetric assays after incub ...
DOI:: 10.1039/c5ra01263e
: https://doi.org/10.1039/c5ra01263e

4. (−)-Loliolide Isolated from Sargassum horneri Abate UVB-Induced Oxidative Damage in Human Dermal Fibroblasts and Subside ECM Degradation

Author:: Ilekuttige Priyan Shanura Fernando; Soo-Jin Heo; Mawalle Kankanamge Hasitha Madhawa Dias; Dissanayaka Mudiyanselage Dinesh Madusanka; Eui-Jeong Han; Min-Ju Kim; Kalu Kapuge Asanka Sanjeewa; Kyounghoon Lee; Ginnae Ahn
Source:: Marine drugs 2021 v.19 no.8 pp. -
ISSN:: 1660-3397
Subject:: Sargassum horneri; apoptosis; collagenase; elastase; extracellular matrix; fibroblasts; humans; ingredients; mitochondria; mitogen-activated protein kinase; necrosis; neoplasms; reactive oxygen species
Abstract:: ... Ultraviolet (UV) B exposure is a prominent cause of skin aging and a contemporary subject of interest. The effects are progressing through the generation of reactive oxygen species (ROS) that alter cell signaling pathways related to inflammatory responses. The present study evaluates the protective effects of (7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one (HTT) isolated from th ...
DOI:: 10.3390/md19080435
: https://doi.org/10.3390/md19080435

5. 032 Cryobanking of testicular tissue in men of reproductive age

Author:: Victoria Keros; Ulrik Kvist
Source:: Cryobiology 2013 v.67 no.3 pp. 407
ISSN:: 0011-2240
Subject:: Leydig cells; Sertoli cells; adolescence; adults; biopsy; cooling; cryopreservation; cryoprotectants; extracellular matrix; freezing; histology; hospitals; humans; ice; intracytoplasmic sperm injection; liquids; medicine; men; neoplasms; nitrogen; patients; puberty; sowing; spermatogenesis; spermatozoa; storage time; straw; temperature; transmission electron microscopy; vitrification; Sweden
Abstract:: ... Cryopreservation of ejaculated or testicular spermatozoa is recommended for fertility preservation in adolescence and adult men. However cryobanking of the properly preserved human testicular tissue in men of reproductive age is an essential source for further clinical application and additional examination for diagnostic purposes. During the last three years 107 infertile patients were referred f ...
DOI:: 10.1016/j.cryobiol.2013.09.038
: https://doi.org/10.1016/j.cryobiol.2013.09.038

6. 1, 25-Dihydroxyvitamin D3 Altered Metabolism of MCF10A-ras Cells Decreases Viability in Extracellular Matrix Detached Conditions (FS13-05-19)

Author:: Madeline Sheeley; Dorothy Teegarden; Katie Wong
Source:: Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:: 2475-2991
Subject:: breast neoplasms; breasts; cell viability; colorimetry; death; diagnostic techniques; energy metabolism; epithelial cells; ethanol; extracellular matrix; gene expression; genes; glutamine; human cell lines; messenger RNA; metabolites; metastasis; neoplasm cells; nutrient content; oxaloacetic acid; pyruvate carboxylase; quantitative polymerase chain reaction; triacylglycerols; tricarboxylic acid cycle; vitamin D
Abstract:: ... Metastasis is the main cause of most breast cancer related deaths. Throughout the process of metastasis, cancer cells strive to survive in conditions where they are detached from the extracellular matrix (ECM). Vitamin D may play a role in preventing breast cancer metastasis by modulating metabolism in breast cancer cells detached from ECM. Here we hypothesize that the active metabolite of vitamin ...
DOI:: 10.1093/cdn/nzz030.FS13-05-19
: https://doi.org/10.1093/cdn/nzz030.FS13-05-19

7. 111 Cryopreservation of stem cells inside alginate 3D constructs after high-voltage encapsulation

Author:: O. Gryshkov; D. Pogozhykh; N. Hofmann; T. Mueller; B. Glasmacher
Source:: Cryobiology 2013 v.67 no.3 pp. 429
ISSN:: 0011-2240
Subject:: Callithrix jacchus; air flow; alginates; analysis of variance; biochemical pathways; cooling; cryopreservation; cryoprotectants; electric potential difference; electrospraying; encapsulation; extracellular matrix; gels; heat transfer; mass transfer; medicine; mesenchymal stromal cells; microbeads; monkeys; sodium citrate; stem cells; storage temperature; storage time; surface area; thawing; toxicity; toxicity testing; viability; viscosity; water content
Abstract:: ... Application of stem cells in regenerative medicine require improving cryopreservation protocols for long term storage, especially for tissue-imitating 3D constructs containing cells. Encapsulation of stem cells into alginate-based 3D constructs that repeat an extracellular matrix may provide a mild environment during cryopreservation as well as the possibility for large-scale expansion. Furthermor ...
DOI:: 10.1016/j.cryobiol.2013.09.117
: https://doi.org/10.1016/j.cryobiol.2013.09.117

8. 116 In Vitro Transcriptomic Response of Bovine Oviduct Epithelial Cells to Direct or Indirect Embryo Contact

Author:: M. Hamdi; B. Rodríguez-Alonso; A. Almansa-Ordonez; A. Gutierrez-Adán; P. Lonergan; D. Rizos
Source:: Reproduction, fertility, and development 2018 v.30 no.1 pp. 197-198
ISSN:: 1031-3613
Subject:: air; analysis of variance; carbon dioxide; cattle; coculture; cytokinesis; direct contact; epithelial cells; essential genes; extracellular matrix; fetal bovine serum; gene expression; indirect contact; inflammation; oviducts; oxidative stress; polyesters; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; sodium; somatomedins; transcriptome; transcriptomics
Abstract:: ... We observed that in vitro transcriptomic response of bovine oviduct epithelial cells (BOEC) to the early embryo could be the result of a contact-dependent signalling effect or interactions with embryo secretions. In order to determine this, BOEC were co-cultured directly with embryos or indirectly with embryo-conditioned media (CM); BOEC from the isthmus of oviducts at early luteal phase were cult ...
DOI:: 10.1071/RDv30n1Ab115
: https://doi.org/10.1071/RDv30n1Ab115

9. 12. From ice in the veins, through unfrozen fish and frozen frogs to isochoric preservation, ad Astra

Author:: Boris Rubinsky
Source:: Cryobiology 2015 v.71 no.1 pp. 167-168
ISSN:: 0011-2240
Subject:: cell membranes; research; fish; heart; extracellular matrix; freezing; transportation; glucose; mechanical damage; solidification; ice; antifreeze proteins; frogs; glycerol; temperature; Lithobates sylvaticus; liver; cryopreservation; cold tolerance; thawing; Arctic region; Antarctic region
Abstract:: ... Star Wars V: The Empire Strikes Back – One of the most dramatic scenes in the movie is when the Dark Lord successfully entombs Han Solo in carbonite in a state of suspended animation for transportation to Tatooine to receive a bounty from Jabba. Recently records show that the film has made so far $819,808,300. I doubt that the entire scientific community, together, through the last century, has re ...
DOI:: 10.1016/j.cryobiol.2015.05.018
: https://doi.org/10.1016/j.cryobiol.2015.05.018

10. 142 Nulliparity alters gene expression in inner cell mass and trophoblast of equine blastocysts in old mares

Author:: E. Derisoud; L. Jouneau; C. Archilla; N. Daniel; Y. Jaszczyszyn; C. Gourtay; A. Margat; M. Dahirel; N. Peynot; L. Briot; F. De Geoffroy; V. Duranthon; P. Chavatte-Palmer
Source:: Reproduction, fertility, and development 2021 v.33 no.2 pp. 179
ISSN:: 1031-3613
Subject:: DNA; RNA; embryonic mortality; extracellular matrix; foals; focal adhesions; gene expression; gene expression regulation; gene ontology; genes; immune response; mares; models; myosin; nucleosomes; plasma membrane; proteasome endopeptidase complex; protein metabolism; ribosomes; semen; sphingolipids; stallions; trophoblast
Abstract:: ... An increased incidence in early embryo loss has been observed in aged mares. Moreover, the first foal born to a mare is lighter than her subsequent foals, with reported impaired placental function at term. Because trophoblast function may be affected from the embryo stage, the aim of this project was to determine the effect of parity in aged mares on gene expression in Day-8.5 embryos. Middle-aged ...
DOI:: 10.1071/RDv33n2Ab142
: https://doi.org/10.1071/RDv33n2Ab142

11. 17β-Estradiol alleviates intervertebral disc degeneration by inhibiting NF-κB signal pathway

Author:: Haidong Wang; Zhaohui Li; Yachong Huo; Tao Tian; Dalong Yang; Lei Ma; Sidong Yang; Wenyuan Ding
Source:: Life sciences 2021 v.284 pp. 119874
ISSN:: 0024-3205
Subject:: X-radiation; antagonists; apoptosis; blood serum; eosin; estrogen receptors; extracellular matrix; fluorescent antibody technique; immunohistochemistry; intervertebral disks; magnetism; metalloproteinases; models; ovariectomy; rats; signal transduction
Abstract:: ... To investigate the effect of 17β-Estradiol (E2) on intervertebral disc degeneration (IVDD) and the related mechanism.Immunohistochemistry was used to detect the expression of estrogen receptor β (ERβ) within intervertebral discs of humans and rats. After that, rat IVDD model was established by needle puncture and bilateral ovariectomy. Then, the serum E2 level was detected by enzyme linked immunos ...
DOI:: 10.1016/j.lfs.2021.119874
: https://doi.org/10.1016/j.lfs.2021.119874

12. 186 The effects of different concentrations of MgSO4 in osteogenic differentiation

Author:: L. R. Padoveze; M. Rubessa; C. E. Ambrosio; M. B. Wheeler
Source:: Reproduction, fertility, and development 2019 v.31 no.1 pp. 217-218
ISSN:: 1031-3613
Subject:: alizarin; bone formation; calcium; extracellular matrix; gene expression regulation; genes; in vitro studies; linear models; magnesium; magnesium sulfate; mesenchymal stromal cells; mineralization; staining; stem cells; swine; tissue engineering
Abstract:: ... Tissue engineering offers a viable alternative to bone grafts in repairing large bone defects. Magnesium-based materials are biocompatible in vivo, and it is possible to determine the degradation period according to the necessities (Farraro et al. 2014 J. Biomech. 47, 1979-1986). Magnesium (Mg) is part of many physiological processes, and it promotes the osteogenesis of mesenchymal stem cells (Día ...
DOI:: 10.1071/RDv31n1Ab186
: https://doi.org/10.1071/RDv31n1Ab186

13. 18β-Glycyrrhetinic acid inhibits IL-1β-induced inflammatory response in mouse chondrocytes and prevents osteoarthritic progression by activating Nrf2

Author:: Boda Chen; Dingchao Zhu; Chenglong Xie; Yifeng Shi; Libin Ni; Huawei Zhang; Sunlong Li; Jiajie Lu; Jian Xiao; Weiyi Xia; Chongan Huang; Xiangyang Wang
Source:: Food & function 2021 v.12 no.18 pp. 8399-8410
ISSN:: 2042-650X
Subject:: Glycyrrhiza glabra; basic-leucine zipper transcription factors; bioactive compounds; cartilage; chondrocytes; collagen; extracellular matrix; histology; inducible nitric oxide synthase; inflammation; interleukin-6; matrix metalloproteinase 13; mice; necrosis; neoplasms; nitric oxide; osteoarthritis; prostaglandin synthase; prostaglandins; sclerosis; synovitis; therapeutics; transcription factor NF-kappa B
Abstract:: ... Osteoarthritis (OA) is presently the most prevalent form of chronic degenerative joint disease, which is characterized by erosion of articular cartilage, subchondral bone sclerosis and synovitis. Accumulating evidence has revealed that 18β-glycyrrhetinic acid (18β-GA), a major bioactive component derived from Glycyrrhiza glabra, exerts anti-inflammatory effects on several diseases. However, the an ...
DOI:: 10.1039/d1fo01379c
: https://doi.org/10.1039/d1fo01379c

14. 1H NMR spectroscopic studies establish that heparanase is a retaining glycosidase

Author:: Jennifer C. Wilson; Andrew Elohim Laloo; Sanjesh Singh; Vito Ferro
Source:: Biochemical and biophysical research communications 2014 v.443 pp. 185-188
ISSN:: 0006-291X
Subject:: angiogenesis; anti-inflammatory agents; extracellular matrix; glycosides; heparan sulfate; hydrolysis; inflammation; metastasis; mutagenicity; nuclear magnetic resonance spectroscopy; prediction; proteoglycans; sequence analysis; spectral analysis
Abstract:: ... Heparanase is an endo-β-glucuronidase that cleaves heparan sulfate side chains of proteoglycans in basement membranes and the extracellular matrix (ECM). Heparanase is implicated in several diverse pathological processes associated with ECM degradation such as metastasis, inflammation and angiogenesis and is thus an important target for anti-cancer and anti-inflammatory drug discovery. Heparanase ...
DOI:: 10.1016/j.bbrc.2013.11.079
: https://doi.org/10.1016/j.bbrc.2013.11.079

15. 1α,25-Dihydroxyvitamin D3 and rosiglitazone synergistically enhance osteoblast-mediated mineralization

Author:: V.J. Woeckel; C. Bruedigam; M. Koedam; H. Chiba; B.C.J. van der Eerden; J.P.T.M. van Leeuwen
Source:: Gene 2013 v.512 pp. 438-443
ISSN:: 0378-1119
Subject:: agonists; alkaline phosphatase; extracellular matrix; gene expression; genes; humans; mineralization; osteoblasts; transcription factors; vitamin D
Abstract:: ... Both vitamin D receptor (VDR) and peroxisome proliferator-activated receptor γ (PPAR-γ) are ligand-activated nuclear transcription factors that are instrumental for bone health. While 1α,25-dihydroxyvitamin D3 (1,25D3), the ligand for VDR, is essential for the development and maintenance of healthy bone, PPAR-γ agonists cause detrimental skeletal effects. Recent studies have revealed evidence for ...
DOI:: 10.1016/j.gene.2012.07.051
PubMed:: 22967709
: https://doi.org/10.1016/j.gene.2012.07.051

16. 1α,25-dihydroxyvitamin D3 Attenuates TGF-β-Induced Pro-Fibrotic Effects in Human Lung Epithelial Cells through Inhibition of Epithelial–Mesenchymal Transition

Author:: Fei Jiang; Yong Yang; Lian Xue; Bingyan Li; Zengli Zhang
Source:: Nutrients 2017 v.9 no.9 pp. -
ISSN:: 2072-6643
Subject:: beta catenin; blood serum; cadherins; calcitriol receptors; cell movement; collagen; epidemiological studies; epithelial cells; epithelium; extracellular matrix; fibronectins; fibrosis; genes; humans; lungs; phenotype; prognosis; respiratory tract diseases; tissue repair; transcription factors; transforming growth factor beta; vimentin; vitamin D
Abstract:: ... Pulmonary fibrosis is a progressive fibrotic lung disease of persisting lung injury and ineffective wound repair, with poor prognosis. Epithelial–mesenchymal transition (EMT) of alveolar epithelia cells is an early event in the development of pulmonary fibrosis, and transforming growth factor β (TGF-β) is an acknowledged inducer of EMT. Epidemiological studies demonstrated that serum levels of 25- ...
DOI:: 10.3390/nu9090980
PubMed:: 28878195
PubMed Central:: PMC5622740
: https://doi.org/10.3390/nu9090980

17. 1α,25-dihydroxyvitamin D3 attenuates oxidative stress-induced damage in human trabecular meshwork cells by inhibiting TGFβ-SMAD3-VDR pathway

Author:: Yingjuan Lv; Xiaohui Han; Qingbin Yao; Kun Zhang; Lina Zheng; Wei Hong; Xiaoli Xing
Source:: Biochemical and biophysical research communications 2019 v.516 no.1 pp. 75-81
ISSN:: 0006-291X
Subject:: Primates; apoptosis; biochemical mechanisms; calcitriol receptors; cholecalciferol; extracellular matrix; glaucoma; humans; hydrogen peroxide; inflammation; models; oxidative stress; therapeutics; transforming growth factor beta
Abstract:: ... Evidence indicates that 1α, 25-dihydroxy vitamin D3 (1, 25-(OH)2D3) markedly reduces intraocular pressure (IOP) in nonhuman primates, while the biochemical mechanisms are unclear. To investigate the influence of oxidative stress on human trabecular meshwork cells (HTMCs) and the effect and regulatory mechanism of 1, 25-(OH)2D3 in HTMCs under oxidative stress, we established an oxidative stress mod ...
DOI:: 10.1016/j.bbrc.2019.06.027
: https://doi.org/10.1016/j.bbrc.2019.06.027

18. 2,5-Dimethoxy 2,5-dihydrofuran crosslinked chitosan fibers enhance bone regeneration in rabbit femur defects

Author:: Paulomi Ghosh; Arun Prabhu Rameshbabu; Nantu Dogra; Santanu Dhara
Source:: RSC advances 2014 v.4 no.37 pp. 19516-19524
ISSN:: 2046-2069
Subject:: adhesion; adsorption; alkaline phosphatase; animal models; bone formation; bone marrow; calcium; cell proliferation; chemical bonding; chitosan; crosslinking; extracellular matrix; femur; glucosamine; histology; humans; hydrophobicity; imines; in vivo studies; mesenchymal stromal cells; neutralization; osteocalcin; pH; polymers; rabbits; secretion; sodium hydroxide; tensile strength; tissue engineering
Abstract:: ... Chitosan fibers were fabricated via pH induced neutralization and precipitation in a 5 w/v% NaOH bath. Intermolecular covalent crosslinking of these fibers were performed through imine linkages between the glucosamine units of polymers and the dialdehyde groups of 2,5-dimethoxy-2,5-dihydrofuran at 60 °C, pH 2.2. The covalently crosslinked fibers demonstrated improved tensile strength, stiffness, h ...
DOI:: 10.1039/c4ra01971g
: https://doi.org/10.1039/c4ra01971g

19. 2-Alkyl-anthraquinones inhibit Candida albicans biofilm via inhibiting the formation of matrix and hyphae

Author:: Yuanyuan Song; Ziqi Wang; Yijing Long; Yang Mao; Feng Jiang; Yuanyuan Lu
Source:: Research in microbiology 2022 pp. 103955
ISSN:: 0923-2508
Subject:: Candida albicans; anthraquinones; biofilm; extracellular matrix; glycosides; hyphae; microbiology; research; transcriptomics; virulence
Abstract:: ... Candida albicans can form biofilm on biotic and abiotic surfaces of medical implants to cause superficial and systemic infections under specific condition. The formation of hyphae and matrix of C. albicans are considered as probable virulence factors. We assessed the inhibitory activities of 26 anthraquinones against C. albicans biofilm formation, which were substituted by different functional gro ...
DOI:: 10.1016/j.resmic.2022.103955
: https://doi.org/10.1016/j.resmic.2022.103955

20. 20(S)- Protopanaxadiol suppresses hepatic stellate cell activation via WIF1 demethylation-mediated inactivation of the Wnt/β-catenin pathway

Author:: Chunxue Li; Yating Zhan; Rongrong Zhang; Qiqi Tao; Zhichao Lang; Jianjian Zheng
Source:: Journal of Ginseng Research 2022 pp. -
ISSN:: 1226-8453
Subject:: DNA methyltransferase; Panax; carbon tetrachloride; collagen; demethylation; drugs; extracellular matrix; liver; liver cirrhosis; methylation; research; therapeutics
Abstract:: ... 20(S)-protopanaxadiol (PPD), one of the main components of ginseng, has anti-inflammatory, anti-estrogenic, and anti-tumor activities. It is known that activated hepatic stellate cells (HSCs) are the primary producers of extracellular matrix (ECM) in the liver, and the Wnt/β-catenin pathway participates in the activation of HSCs. We aimed to explore whether PPD inhibits liver fibrosis is associate ...
DOI:: 10.1016/j.jgr.2022.05.005
: https://doi.org/10.1016/j.jgr.2022.05.005

21. 20S-Protopanaxatriol Ameliorates Hepatic Fibrosis, Potentially Involving FXR-Mediated Inflammatory Signaling Cascades

Author:: Jian Song; Zhen-Yu Cui; Li-Hua Lian; Xin Han; Li-Shuang Hou; Ge Wang; Lu Gao; Yue Zhu; Yu-Chen Jiang; Jia-Yi Dou; Zhong-He Hu; Yu-Qing Zhao; Ji-Xing Nan; Yan-Ling Wu
Source:: Journal of agricultural and food chemistry 2020 v.68 no.31 pp. 8195-8204
ISSN:: 1520-5118
Subject:: Panax; agonists; caspase-1; extracellular matrix; fibrosis; food chemistry; functional foods; histopathology; inflammation; interleukin-6; ion channels; liver cirrhosis; metabolites; protective effect; secretion
Abstract:: ... Ginseng has been used as a functional food and tonic for enhancing immune power. Here, the potential protective effect of 20S-protopanaxatriol (M4), the metabolite of protopanaxatriol, against hepatic fibrosis is investigated, which could provide nutritional interventions for disease treatment. M4 could inhibit extracellular matrix (ECM) deposition and reduce the levels of proinflammatory cytokine ...
DOI:: 10.1021/acs.jafc.0c01978
: https://doi.org/10.1021/acs.jafc.0c01978

22. 217 Induction and differentiation of porcine induced pluripotent stem cells into neuronal precursor cell-like cells

Author:: L. S. Machado; N. C. G. Pieri; R. C. Botigelli; R. V. G. Castro; A. F. de Souza; M. A. de Lima; A. Bridi; A. F. C. Andrade; F. V. Meirelles; K. K. Freude; F. F. Bressan
Source:: Reproduction, fertility, and development 2020 v.32 no.2 pp. 236-237
ISSN:: 1031-3613
Subject:: Parkinson disease; alkaline phosphatase; animal models; antibiotics; autism; beta-mercaptoethanol; blood serum; cytoplasm; epidermal growth factor; extracellular matrix; fibroblast growth factor 2; fibroblasts; flow cytometry; fluorescent antibody technique; genes; genetic disorders; glutamine; human diseases; humans; induced pluripotent stem cells; medicine; neurons; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; schizophrenia; swine; translational medical research; tubulin; vimentin
Abstract:: ... The invitro cellular induction into pluripotency [induced pluripotent stem cell (iPSC) generation] has much evolved since its first report and may play a central role in veterinary regenerative medicine. In particular, the generation of invitro neural precursor cells (NPCs) enabled the creation of models for human genetic diseases, such as autism, schizophrenia, and Parkinson disease. The swine mo ...
DOI:: 10.1071/RDv32n2Ab217
: https://doi.org/10.1071/RDv32n2Ab217

23. 22. Avoiding the problems of ice in tissues via vitrification

Author:: Mike Taylor; Kelvin G.M. Brockbank; Yoed Rabin
Source:: Cryobiology 2015 v.71 no.1 pp. 170-171
ISSN:: 0011-2240
Subject:: additives; blood cells; blood vessels; cartilage; cell suspension culture; cooling; cryopreservation; cryoprotectants; crystallization; crystals; dimethyl sulfoxide; extracellular matrix; freezing; heart valves; heat treatment; ice; ice nucleation; islets of Langerhans; kidneys; liquids; mechanical stress; risk; solidification; solutes; stem cells; temperature; toxicity; viscosity; vitrification
Abstract:: ... Cryopreservation involving freezing in the presence of protective agents such as dimethyl sulfoxide is a well-established method for storing cells and small tissues. While successful techniques for cryopreservation have been developed over the past seven decades, they are generally related to small specimens, in the scale-range of cell suspensions and clusters to small-organized tissues (μm to mm ...
DOI:: 10.1016/j.cryobiol.2015.05.028
: https://doi.org/10.1016/j.cryobiol.2015.05.028

24. 244 chondrogenic pellet cultures for cartilage tissue engineering grow by deposition of matrix and not by cellular proliferation

Author:: S. Johnson; D. Milner; H. Lopez-Lake; M. Wheeler
Source:: Reproduction, fertility, and development 2016 v.28 no.2 pp. 254
ISSN:: 1031-3613
Subject:: adults; cartilage; cell nucleus; cell proliferation; centrifugation; chondrocytes; chondrogenesis; chondroitin sulfate; collagen; computer software; dexamethasone; extracellular matrix; fetal bovine serum; image analysis; keratin; pellets; proline; stem cells; sulfates; swine; t-test; tissue engineering
Abstract:: ... Pellet cultures are commonly used to study chondrogenic differentiation in vitro. Our laboratory has demonstrated pellets made with chondrocytes grow in size during culture and produce cartilage matrix, but pellets made with adipose-derived mesenchymal stem cells (ASC) grow only slightly, producing little cartilage matrix. The objective of this study was to determine if differences in chondrocyte ...
DOI:: 10.1071/RDv28n2Ab244
: https://doi.org/10.1071/RDv28n2Ab244

25. 3,4-Methylenedioxy-β-nitrostyrene inhibits adhesion and migration of human triple-negative breast cancer cells by suppressing β1 integrin function and surface protein disulfide isomerase

Author:: I-Hua Chen; Fang-Rong Chang; Yang-Chang Wu; Po-Hsiung Kung; Chin-Chung Wu
Source:: Biochimie 2015 v.110 pp. 81-92
ISSN:: 0300-9084
Subject:: CD29 antigen; Western blotting; actin; adhesion; antineoplastic agents; breast neoplasms; cell adhesion; cell viability; confocal microscopy; extracellular matrix; immigration; metastasis; neoplasm cells; non-specific protein-tyrosine kinase; phosphorylation; protein disulfide-isomerase; surface proteins; thiols
Abstract:: ... Triple negative breast cancer (TNBC) exhibits an aggressive clinical course by high metastatic potential. It is known that integrin-mediated cell adhesion and migration are important for cancer metastasis. In the present study, a synthetic compound, 3, 4-methyenedioxy-β-nitrostyrene (MNS), significantly inhibited adhesion of TNBC cell lines to different extracellular matrix (ECM) components. The a ...
DOI:: 10.1016/j.biochi.2015.01.006
: https://doi.org/10.1016/j.biochi.2015.01.006

26. 3-D mineralized silk fibroin/polycaprolactone composite scaffold modified with polyglutamate conjugated with BMP-2 peptide for bone tissue engineering

Author:: Jingjing Luo; Haitao Zhang; Jiang zhu; Xiaokang Cui; Junjiu Gao; Xin Wang; Jie Xiong
Source:: Colloids and surfaces 2018 v.163 pp. 369-378
ISSN:: 0927-7765
Subject:: Fourier transform infrared spectroscopy; X-ray diffraction; binding properties; bone formation; bones; colloids; extracellular matrix; fibroins; gene expression; genes; glutamic acid; messenger RNA; mineralization; nanofibers; scanning electron microscopy; silk; stem cells; tissue engineering
Abstract:: ... In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated wit ...
DOI:: 10.1016/j.colsurfb.2017.12.043
: https://doi.org/10.1016/j.colsurfb.2017.12.043

27. 3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture

Author:: K. Jiang; D. Chaimov; S.N. Patel; J.-P. Liang; S.C. Wiggins; M.M. Samojlik; A. Rubiano; C.S. Simmons; C.L. Stabler
Source:: Biomaterials 2019 v.198 pp. 37-48
ISSN:: 0142-9612
Subject:: bladder; cell viability; cost effectiveness; diabetes; encapsulation; endothelial cells; extracellular matrix; humans; hydrogels; islets of Langerhans; lungs; organ-on-a-chip; physiology; rats; screening; swine; viscoelasticity
Abstract:: ... Organ-on-a-chip platforms serve as cost-efficient testbeds for screening pharmaceutical agents, mimicking natural physiology, and studying disease. In the field of diabetes, the development of an islet-on-a-chip platform would have broad implications in understanding disease pathology and discovering potential therapies. Islet microphysiological systems are limited, however, by their poor cell sur ...
DOI:: 10.1016/j.biomaterials.2018.08.057
PubMed:: 30224090
PubMed Central:: PMC6397100
: https://doi.org/10.1016/j.biomaterials.2018.08.057

28. 3-Dimensional porous nanocomposite scaffolds based on cellulose nanofibers for cartilage tissue engineering: tailoring of porosity and mechanical performance

Author:: Narges Naseri; Jean-Michel Poirier; Lenart Girandon; Mirjam Fröhlich; Kristiina Oksman; Aji P. Mathew
Source:: RSC advances 2016 v.6 no.8 pp. 5999-6007
ISSN:: 2046-2069
Subject:: cartilage; cellulose nanofibers; chitosan; chondrocytes; chondrogenesis; computed tomography; crosslinking; extracellular matrix; freeze drying; gelatin; mechanical properties; nanocomposites; phosphates; porosity; roughness; scanning electron microscopy; tissue engineering
Abstract:: ... Fully bio-based 3-dimensional porous scaffolds based on freeze-dried cellulose nanofibers (70–90 wt%) stabilized using a genipin crosslinked matrix of gelatin and chitosan were prepared. Morphology studies using scanning electron microscopy showed that the scaffolds have interconnected pores with average pore diameters of 75–200 μm and nanoscaled pore wall roughness, both favorable for cell intera ...
DOI:: 10.1039/c5ra27246g
: https://doi.org/10.1039/c5ra27246g

29. 3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model

Author:: Daniel S. Puperi; Liezl R. Balaoing; Ronan W. O'Connell; Jennifer L. West; K. Jane Grande-Allen
Source:: Biomaterials 2015 v.67 pp. 354-364
ISSN:: 0142-9612
Subject:: cell growth; coculture; direct contact; disease course; endothelial cells; extracellular matrix; heart valve diseases; hydrocolloids; ligands; models; phenotype
Abstract:: ... Physiologically relevant in vitro models are needed to study disease progression and to develop and screen potential therapeutic interventions for disease. Heart valve disease, in particular, has no early intervention or non-invasive treatment because there is a lack of understanding the cellular mechanisms which lead to disease. Here, we establish a novel, customizable synthetic hydrogel platform ...
DOI:: 10.1016/j.biomaterials.2015.07.039
PubMed:: 26241755
PubMed Central:: PMC4566924
: https://doi.org/10.1016/j.biomaterials.2015.07.039

30. 30. Thermal properties of porcine myocardial tissue at subzero temperatures: Implication for thermal therapy studies

Author:: Jeunghwan Choi; Michael Etheridge; Dushyant Mehra; John Bischof
Source:: Cryobiology 2013 v.66 no.3 pp. 350
ISSN:: 0011-2240
Subject:: atrial fibrillation; catheters; cryosurgery; databases; differential scanning calorimetry; energy; extracellular matrix; gels; glass transition; glass transition temperature; heat transfer; ice; latent heat; models; myocardium; nerve tissue; prediction; risk reduction; specific heat; swine; thermal conductivity; thermistors; thrombosis; water
Abstract:: ... Cryoablation for atrial fibrillation is a clinically approved technique used to control irregular heart rhythms due to abnormal electrical signals conducted from the pulmonary veins to the atria. The ablation catheter rapidly cools the cardiac tissue to freezing temperatures creating a discrete lesion which terminates these abnormal electrical pulses. Benefits of this approach include decreased ri ...
DOI:: 10.1016/j.cryobiol.2013.02.036
: https://doi.org/10.1016/j.cryobiol.2013.02.036

31. 3D Bioprinting Using Cross-Linker-Free Silk–Gelatin Bioink for Cartilage Tissue Engineering

Author:: Yogendra Pratap Singh; Ashutosh Bandyopadhyay; Biman B. Mandal
Source:: ACS applied materials & interfaces 2019 v.11 no.37 pp. 33684-33696
ISSN:: 1944-8252
Subject:: Bombyx mori; Philosamia; biocompatibility; bioprinting; cartilage; chondrocytes; collagen; compression strength; crosslinking; encapsulation; extracellular matrix; extrusion; fibroins; gelatin; gene expression; gene expression regulation; glycosaminoglycans; humans; hypertrophy; polymers; quality of life; rheology; silk; tissue engineering; toxic substances
Abstract:: ... Cartilage tissue is deprived of intrinsic self-regeneration capability; hence, its damage often progresses to a chronic condition which reduces the quality of life. Toward the fabrication of functional tissue substitutes, three-dimensional (3D) bioprinting has progressed vastly over the last few decades. However, this progress is challenged by the difficulty in developing suitable bioink materials ...
DOI:: 10.1021/acsami.9b11644
: https://doi.org/10.1021/acsami.9b11644

32. 3D Bone tissue engineered with bioactive microspheres in simulated microgravity

Author:: Qing-Qing Qiu; Paul Ducheyne; Portonovo S. Ayyaswamy
Source:: In vitro cellular & developmental biology 2001 v.37 no.3 pp. 157-165
ISSN:: 1071-2690
Subject:: apatite; biodegradability; bone marrow; cell culture; drug delivery systems; extracellular matrix; glass; in vitro studies; microgravity; mineralization; osteoblasts; polymers; stromal cells; tissue engineering
Abstract:: ... Three-dimensional (3D) osteoblast cell cultures were obtained in rotating-wall vessels (RWV), simulating microgravity. Three types of bioactive microcarriers, specifically modified bioactive glass particles, bioceramic hollow microspheres, and biodegradable bioactive glass-polymer composite microspheres, were developed and used with osteblasts. The surfaces of composite microspheres fully transfor ...
DOI:: 10.1290/1071-2690(2001)037<0157:BTEWBM>2.0.CO;2
: https://doi.org/10.1290/1071-2690(2001)037<0157:BTEWBM>2.0.CO;2

33. 3D Cocultures of Osteoblasts and Staphylococcus aureus on Biomimetic Bone Scaffolds as a Tool to Investigate the Host–Pathogen Interface in Osteomyelitis

Author:: Raffaella Parente; Valentina Possetti; Maria Lucia Schiavone; Elisabetta Campodoni; Ciro Menale; Mattia Loppini; Andrea Doni; Barbara Bottazzi; Alberto Mantovani; Monica Sandri; Anna Tampieri; Cristina Sobacchi; Antonio Inforzato
Source:: Pathogens 2021 v.10 no.7 pp. -
ISSN:: 2076-0817
Subject:: Gram-positive bacteria; Staphylococcus aureus; animal experimentation; biomimetics; cell lines; coculture; collagen; extracellular matrix; gene expression; homeostasis; hydroxyapatite; immune response; infectious diseases; mice; models; opportunistic pathogens; osteoblasts; osteomyelitis; protein synthesis; texture; transcription (genetics)
Abstract:: ... Osteomyelitis (OM) is an infectious disease of the bone primarily caused by the opportunistic pathogen Staphylococcus aureus (SA). This Gram-positive bacterium has evolved a number of strategies to evade the immune response and subvert bone homeostasis, yet the underlying mechanisms remain poorly understood. OM has been modeled in vitro to challenge pathogenetic hypotheses in controlled conditions ...
DOI:: 10.3390/pathogens10070837
: https://doi.org/10.3390/pathogens10070837

34. 3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

Author:: Julien Nicolas; Sofia Magli; Linda Rabbachin; Susanna Sampaolesi; Francesco Nicotra; Laura Russo
Source:: Biomacromolecules 2020 v.21 no.6 pp. 1968-1994
ISSN:: 1526-4602
Subject:: biochemical compounds; chemistry; drugs; extracellular matrix; medicine; stem cells
Abstract:: ... Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tunin ...
DOI:: 10.1021/acs.biomac.0c00045
: https://doi.org/10.1021/acs.biomac.0c00045

35. 3D Microcontact Printing for Combined Chemical and Topographical Patterning on Porous Cell Culture Membrane

Author:: Justyna Borowiec; Joerg Hampl; Sukhdeep Singh; Sebastian Haefner; Karin Friedel; Patrick Mai; Dana Brauer; Florian Ruther; Liliana Liverani; Aldo R. Boccaccini; Andreas Schober
Source:: ACS applied materials & interfaces 2018 v.10 no.26 pp. 22857-22865
ISSN:: 1944-8252
Subject:: adhesion; antibodies; cell culture; extracellular matrix; geometry; materials science; microstructure; proteins; staining
Abstract:: ... Micrometer-scale biochemical or topographical patterning is commonly used to guide the cell attachment and growth, but the ability to combine these patterns into an integrated surface with defined chemical and geometrical characteristics still remains a technical challenge. Here, we present a technical solution for simultaneous construction of 3D morphologies, in the form of channels, on porous me ...
DOI:: 10.1021/acsami.8b06585
: https://doi.org/10.1021/acsami.8b06585

36. 3D Porous poly(lactic acid)/regenerated cellulose composite scaffolds based on electrospun nanofibers for biomineralization

Author:: Juan Chen; Tonghui Zhang; Weikang Hua; Peiyun Li; Xuefen Wang
Source:: Colloids and surfaces 2020 v.585 pp. 124048
ISSN:: 0927-7757
Subject:: apatite; bioactive properties; biomedical materials; biomimetics; biomineralization; bone formation; bones; cell structures; cellulose; citric acid; crosslinking; extracellular matrix; freeze drying; hydrophilicity; moieties; nanofibers; polylactic acid; strength (mechanics); tissue engineering; water uptake
Abstract:: ... One of the serious challenges in bone tissue engineering is the construction of biomimetic scaffolds, which can bridge the gap between mechanical strength and porous structure requirements. Although electrospinning technology can be used to create nanofibrous networks with a structure of artificial extracellular matrix (ECM), the restricted shapes and pore sizes block its application. Herein, we c ...
DOI:: 10.1016/j.colsurfa.2019.124048
: https://doi.org/10.1016/j.colsurfa.2019.124048

37. 3D Scaffolds with Different Stiffness but the Same Microstructure for Bone Tissue Engineering

Author:: Chen Guobao; Dong Chanjuan; Yang Li; Lv Yonggang
Source:: ACS Applied Materials & Interfaces 2015 v.7 no.29 pp. 15790-15802
ISSN:: 1944-8252
Subject:: adhesion; angiogenesis; bone formation; bones; cell adhesion; cell differentiation; cell viability; coatings; collagen; extracellular matrix; hydroxyapatite; mechanical properties; micro-computed tomography; microstructure; osteoblasts; porosity; rats; stem cells; tissue engineering
Abstract:: ... A growing body of evidence has shown that extracellular matrix (ECM) stiffness can modulate stem cell adhesion, proliferation, migration, differentiation, and signaling. Stem cells can feel and respond sensitively to the mechanical microenvironment of the ECM. However, most studies have focused on classical two-dimensional (2D) or quasi-three-dimensional environments, which cannot represent the re ...
DOI:: 10.1021/acsami.5b02662
: https://doi.org/10.1021/acsami.5b02662

38. 3D Tissue Engineered Supramolecular Hydrogels for Controlled Chondrogenesis of Human Mesenchymal Stem Cells

Author:: Jung Hyuntae; Park Ji Sun; Yeom Junseok; Selvapalam Narayanan; Park Kyeng Min; Oh Kyunghoon; Yang Jeong-A; Park Keun Hong; Hahn Sei Kwang; Kim Kimoon
Source:: Biomacromolecules 2014 v.15 no.3 pp. 707-714
ISSN:: 1526-4602
Subject:: cartilage; chondrogenesis; crosslinking; dexamethasone; extracellular matrix; glycosaminoglycans; humans; hydrogels; immunohistochemistry; mechanical properties; quantitative polymerase chain reaction; stem cells; tissue engineering; transforming growth factor beta 3
Abstract:: ... Despite a wide investigation of hydrogels as an artificial extracellular matrix, there are few scaffold systems for the facile spatiotemporal control of mesenchymal stem cells (MSCs). Here, we report 3D tissue engineered supramolecular hydrogels prepared with highly water-soluble monofunctionalized cucurbit[6]uril–hyaluronic acid (CB[6]-HA), diaminohexane conjugated HA (DAH-HA), and drug conjugate ...
DOI:: 10.1021/bm401123m
: https://doi.org/10.1021/bm401123m

39. 3D Toolbox to Enhance Physiological Relevance of Human Tissue Models

Author:: Nathalie Picollet-D’hahan; Monika E. Dolega; Lavinia Liguori; Christophe Marquette; Séverine Le Gac; Xavier Gidrol; Donald K. Martin
Source:: Trends in biotechnology 2016 pp. -
ISSN:: 0167-7799
Subject:: engineering; animal use replacement; humans; organ-on-a-chip; drugs; models; cell harvesting; exocrine glands; screening; extracellular matrix
Abstract:: ... We discuss the current challenges and future prospects of flow-based organoid models and 3D self-assembling scaffolds. The existing paradigm of 3D culture suffers from a lack of control over organoid size and shape; can be an obstacle for cell harvesting and extended cellular and molecular analysis; and does not provide access to the function of exocrine glands. Moreover, existing organ-on-chip mo ...
DOI:: 10.1016/j.tibtech.2016.06.012
: https://doi.org/10.1016/j.tibtech.2016.06.012

40. 3D bio-printed biphasic scaffolds with dual modification of silk fibroin for the integrated repair of osteochondral defects

Author:: Changxu Deng; Jin Yang; Hongtao He; Zhenjiang Ma; Wenhao Wang; Yuxin Zhang; Tao Li; Chuanglong He; Jinwu Wang
Source:: Biomaterials science 2021 v.9 no.14 pp. 4891-4903
ISSN:: 2047-4849
Subject:: anhydrides; biocompatible materials; bioprinting; cartilage; chemical bonding; chondrocytes; extracellular matrix; fibroins; gelatin; hypertrophy; models; parathyroid hormone; phenotype; rabbits
Abstract:: ... Repair of osteochondral defects is still a challenge, especially the regeneration of hyaline cartilage. Parathyroid hormone (PTH) can inhibit the hypertrophy of chondrocytes to maintain the phenotype of hyaline cartilage. Here, we aimed to construct a bio-printed biphasic scaffold with a mechanical gradient based on dual modification of silk fibroin (SF) for the integrated repair of osteochondral ...
DOI:: 10.1039/d1bm00535a
: https://doi.org/10.1039/d1bm00535a

41. 3D bioprinting dermal-like structures using species-specific ulvan

Author:: Xifang Chen; Zhilian Yue; Pia C. Winberg; Yan-Ru Lou; Stephen Beirne; Gordon G. Wallace
Source:: Biomaterials science 2021 v.9 no.7 pp. 2424-2438
ISSN:: 2047-4849
Subject:: biocompatibility; biocompatible materials; bioprinting; cell proliferation; cell viability; collagen; elastin; extracellular matrix; extrusion; fibroblasts; fibronectins; gelatin; glycosaminoglycans; humans; macroalgae; strength (mechanics); three-dimensional printing; tissue repair
Abstract:: ... 3D bioprinting has been increasingly employed in skin tissue engineering for manufacturing living constructs with three-dimensional spatial precision and controlled architecture. There is however, a bottleneck in the tunability of bioinks to address specific biocompatibility challenges, functional traits and printability. Here we report on a traditional gelatin methacryloyl (GelMA) based bioink, t ...
DOI:: 10.1039/d0bm01784a
: https://doi.org/10.1039/d0bm01784a

42. 3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering

Author:: Byoung Soo Kim; Yang Woo Kwon; Jeong-Sik Kong; Gyu Tae Park; Ge Gao; Wonil Han; Moon-Bum Kim; Hyungseok Lee; Jae Ho Kim; Dong-Woo Cho
Source:: Biomaterials 2018 v.168 pp. 38-53
ISSN:: 0142-9612
Subject:: angiogenesis; biocompatible materials; biofabrication; bioprinting; blood flow; cytokines; extracellular matrix; growth factors; manufacturing; mechanical properties; models; secretion; shrinkage; skin (animal); stem cells; tissue culture; tissue engineering
Abstract:: ... 3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rel ...
DOI:: 10.1016/j.biomaterials.2018.03.040
: https://doi.org/10.1016/j.biomaterials.2018.03.040

43. 3D cell printing of islet-laden pancreatic tissue-derived extracellular matrix bioink constructs for enhancing pancreatic functions

Author:: Jaewook KimThese authors contributed equally to this work.; In Kyong Shim; Dong Gyu Hwang; Yu Na Lee; Myungji Kim; Hyeonji Kim; Seok-Won Kim; Song Lee; Song Cheol Kim; Dong-Woo Cho; Jinah Jang
Source:: Journal of materials chemistry B 2019 v.7 no.10 pp. 1773-1781
ISSN:: 2050-7518
Subject:: bioprinting; chemistry; coculture; diabetes; endothelial cells; extracellular matrix; humans; hypoglycemia; insulin; insulin secretion; necrosis; pancreas; patients; stem cells; therapeutics; tissues
Abstract:: ... Type 1 diabetes mellitus (T1DM) is a form of diabetes that inhibits or halts insulin production in the pancreas. Although various therapeutic options are applied in clinical settings, not all patients are treatable with such methods due to the instability of the T1DM or the unawareness of hypoglycemia. Islet transplantation using a tissue engineering-based approach may mark a clinical significance ...
DOI:: 10.1039/c8tb02787k
: https://doi.org/10.1039/c8tb02787k

44. 3D cell-printing of biocompatible and functional meniscus constructs using meniscus‐derived bioink

Author:: Suhun Chae; Sung-Sahn Lee; Yeong-Jin Choi; Da Hee Hong; Ge Gao; Joon Ho Wang; Dong-Woo Cho
Source:: Biomaterials 2021 v.267 pp. 120466
ISSN:: 0142-9612
Subject:: anisotropy; biocompatibility; biocompatible materials; encapsulation; extracellular matrix; orthopedics; polyurethanes
Abstract:: ... Meniscus injuries are prevalent in orthopedic diagnosis. The reconstruction of the structural inhomogeneity and anisotropy of the meniscus is a major challenge in clinical practice. Meniscal tissue engineering has emerged as a potential alternative for the treatment of various meniscal diseases and injuries. In this study, we developed three-dimensional (3D) cell-printed meniscus constructs using ...
DOI:: 10.1016/j.biomaterials.2020.120466
: https://doi.org/10.1016/j.biomaterials.2020.120466

45. 3D heterogeneous islet organoid generation from human embryonic stem cells using a novel engineered hydrogel platform

Author:: Joseph Candiello; Taraka Sai Pavan Grandhi; Saik Kia Goh; Vimal Vaidya; Maya Lemmon-Kishi; Kiarash Rahmani Eliato; Robert Ros; Prashant N. Kumta; Kaushal Rege; Ipsita Banerjee
Source:: Biomaterials 2018 v.177 pp. 27-39
ISSN:: 0142-9612
Subject:: c-peptide; embryonic stem cells; endothelial cells; extracellular matrix; genes; glucose; humans; hydrogels; islets of Langerhans; protein synthesis; reproduction
Abstract:: ... Organoids, which exhibit spontaneous organ specific organization, function, and multi-cellular complexity, are in essence the in vitro reproduction of specific in vivo organ systems. Recent work has demonstrated human pluripotent stem cells (hPSCs) as a viable regenerative cell source for tissue-specific organoid engineering. This is especially relevant for engineering islet organoids, due to the ...
DOI:: 10.1016/j.biomaterials.2018.05.031
: https://doi.org/10.1016/j.biomaterials.2018.05.031

46. 3D multi-layered fibrous cellulose structure using an electrohydrodynamic process for tissue engineering

Author:: Minseong Kim; GeunHyung Kim
Source:: Journal of colloid and interface science 2015 v.457 pp. 180-187
ISSN:: 0021-9797
Subject:: biobased products; biochemical pathways; cell adhesion; cellulose; cellulosic fibers; electric field; extracellular matrix; fibroblasts; geometry; humans; nanofibers; tissue engineering; topology
Abstract:: ... Micro/nanofibrous structures have been applied widely in various tissue-engineering applications because the topological structures are similar to the extracellular matrix (ECM), which encourages a high degree of cell adhesion and growth. However, it has been difficult to produce a three-dimensional (3D) fibrous structure using controllable macro-pores. Recently, cellulose has been considered a hi ...
DOI:: 10.1016/j.jcis.2015.07.007
: https://doi.org/10.1016/j.jcis.2015.07.007

47. 3D organizational mapping of collagen fibers elucidates matrix remodeling in a hormone-sensitive 3D breast tissue model

Author:: Zhiyi Liu; Lucia Speroni; Kyle P. Quinn; Carlo Alonzo; Dimitra Pouli; Yang Zhang; Emily Stuntz; Carlos Sonnenschein; Ana M. Soto; Irene Georgakoudi
Source:: Biomaterials 2018 v.179 pp. 96-108
ISSN:: 0142-9612
Subject:: breasts; collagen; epithelium; estradiol; extracellular matrix; hormonal regulation; image analysis; models; morphogenesis; prolactin; tissue culture; variance
Abstract:: ... Hormones play an important role in normal and diseased breast tissue development. However, they can also disrupt cell-matrix interactions and their role in extracellular matrix reorganization during epithelial morphogenesis remains poorly understood, partly due to a lack of sensitive approaches for matrix characterization. Here, we assess the hormonal regulation of matrix reorganization in a three ...
DOI:: 10.1016/j.biomaterials.2018.06.036
PubMed:: 29980078
PubMed Central:: PMC6176712
: https://doi.org/10.1016/j.biomaterials.2018.06.036

48. 3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells

Author:: Kailei Xu; Zi Wang; John A. Copland; Ratna Chakrabarti; Stephen J. Florczyk
Source:: Biomaterials 2020 v.254 pp. 120126
ISSN:: 0142-9612
Subject:: biocompatible materials; cadherins; chitosan; chondroitin sulfate; drug resistance; epithelium; extracellular matrix; metastasis; porosity; prognosis; prostatic neoplasms; sulfates; vimentin
Abstract:: ... Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens. Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa. Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications. Three-dimensional porous chitosan-CS (C-CS) scaf ...
DOI:: 10.1016/j.biomaterials.2020.120126
: https://doi.org/10.1016/j.biomaterials.2020.120126

49. 3D porous scaffolds from wheat glutenin for cultured meat applications

Author:: Ning Xiang; John S.K. Yuen; Andrew J. Stout; Natalie R. Rubio; Ying Chen; David L. Kaplan
Source:: Biomaterials 2022 v.285 pp. 121543
ISSN:: 0142-9612
Subject:: biocompatible materials; cattle; crosslinking; cultured meat; extracellular matrix; glutenins; mice; myoblasts; plant source protein; refrigeration; toxicity; wheat
Abstract:: ... Scaffolds suitable for use in food products are crucial components for the production of cultured meat. Here, wheat glutenin, an inexpensive and abundant plant-based protein, was used to develop 3D porous scaffolds for cultured meat applications. A physical cross-linking method based on water annealing was developed for the fabrication of porous glutenin sponges and fibrous aligned scaffolds. The ...
DOI:: 10.1016/j.biomaterials.2022.121543
: https://doi.org/10.1016/j.biomaterials.2022.121543

50. 3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac repair

Author:: Jinah Jang; Hun-Jun Park; Seok-Won Kim; Heejin Kim; Ju Young Park; Soo Jin Na; Hyeon Ji Kim; Moon Nyeo Park; Seung Hyun Choi; Sun Hwa Park; Sung Won Kim; Sang-Mo Kwon; Pum-Joon Kim; Dong-Woo Cho
Source:: Biomaterials 2017 v.112 pp. 264-274
ISSN:: 0142-9612
Subject:: extracellular matrix; fibrosis; heart diseases; hypertrophy; infarction; myocardium; stem cells; therapeutics; tissue repair
Abstract:: ... Stem cell therapy is a promising therapeutic method for the treatment of ischemic heart diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In t ...
DOI:: 10.1016/j.biomaterials.2016.10.026
: https://doi.org/10.1016/j.biomaterials.2016.10.026

51. 3D scaffolds in breast cancer research

Author:: Girdhari Rijal; Weimin Li
Source:: Biomaterials 2016 v.81 pp. 135-156
ISSN:: 0142-9612
Subject:: adipocytes; blood vessels; breast neoplasms; cell growth; drugs; extracellular matrix; metastasis; neoplasm cells; physical properties; stromal cells
Abstract:: ... Breast tumors grow in a tissue microenvironment containing extracellular matrix (ECM), adipocytes, stromal cells, fluids, and blood vessels. This natural yet complex physiopathological territory is dynamically remodeled in favor of tumor growth and metastasis. The environment-mimicking 3D cultures have shown compelling advantages in the studies of tumor cell biology, and are of intensive research ...
DOI:: 10.1016/j.biomaterials.2015.12.016
: https://doi.org/10.1016/j.biomaterials.2015.12.016

52. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes

Author:: Marco Campisi; Yoojin Shin; Tatsuya Osaki; Cynthia Hajal; Valeria Chiono; Roger D. Kamm
Source:: Biomaterials 2018 v.180 pp. 117-129
ISSN:: 0142-9612
Subject:: astrocytes; blood-brain barrier; brain; clinical trials; confocal microscopy; drugs; endothelial cells; extracellular matrix; fibrin; gels; humans; immunocytochemistry; models; neurodegenerative diseases; pathogens; permeability; rats; tight junctions; transporters
Abstract:: ... The blood-brain barrier (BBB) regulates molecular trafficking, protects against pathogens, and prevents efficient drug delivery to the brain. Models to date failed to reproduce the human anatomical complexity of brain barriers, contributing to misleading results in clinical trials. To overcome these limitations, a novel 3-dimensional BBB microvascular network model was developed via vasculogenesis ...
DOI:: 10.1016/j.biomaterials.2018.07.014
PubMed:: 30032046
PubMed Central:: PMC6201194
: https://doi.org/10.1016/j.biomaterials.2018.07.014

53. 3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment

Author:: Sofia P. Rebelo; Catarina Pinto; Tatiana R. Martins; Nathalie Harrer; Marta F. Estrada; Pablo Loza-Alvarez; José Cabeçadas; Paula M. Alves; Emilio J. Gualda; Wolfgang Sommergruber; Catarina Brito
Source:: Biomaterials 2018 v.163 pp. 185-197
ISSN:: 0142-9612
Subject:: alginates; cell lines; cell movement; chemokine CCL22; chemokine CCL24; chemokine CXCL1; coculture; collagen; disease course; drug therapy; drugs; extracellular matrix; fibroblasts; fibronectins; immunosuppression; immunotherapy; interleukin-10; interleukin-4; lung neoplasms; macrophages; metalloproteinases; microencapsulation; monocytes; neoplasm cells; phenotype; prediction
Abstract:: ... The tumour microenvironment (TME) shapes disease progression and influences therapeutic response. Most aggressive solid tumours have high levels of myeloid cell infiltration, namely tumour associated macrophages (TAM). Recapitulation of the interaction between the different cellular players of the TME, along with the extracellular matrix (ECM), is critical for understanding the mechanisms underlyi ...
DOI:: 10.1016/j.biomaterials.2018.02.030
: https://doi.org/10.1016/j.biomaterials.2018.02.030

54. 3D-Bioprinted Difunctional Scaffold for In Situ Cartilage Regeneration Based on Aptamer-Directed Cell Recruitment and Growth Factor-Enhanced Cell Chondrogenesis

Author:: Zhen Yang; Tianyuan Zhao; Cangjian Gao; Fuyang Cao; Hao Li; Zhiyao Liao; Liwei Fu; Pinxue Li; Wei Chen; Zhiqiang Sun; Shuangpeng Jiang; Zhuang Tian; Guangzhao Tian; Kangkang Zha; Tingting Pan; Xu Li; Xiang Sui; Zhiguo Yuan; Shuyun Liu; Quanyi Guo
Source:: ACS applied materials & interfaces 2021 v.13 no.20 pp. 23369-23383
ISSN:: 1944-8252
Subject:: biodegradability; bioprinting; cartilage; cell adhesion; chondrogenesis; extracellular matrix; gelatin; oligonucleotides; polymers; rabbits; strength (mechanics); therapeutics
Abstract:: ... Articular cartilage (AC) lesions are fairly common but remain an obstacle for clinicians and researchers due to their poor self-healing capacity. Recently, a promising therapy based on the recruitment of autologous mesenchymal stem cells (MSCs) has been developed for the regeneration of full-thickness cartilage defects in the knee joint. In this study, a 3D-bioprinted difunctional scaffold was dev ...
DOI:: 10.1021/acsami.1c01844
: https://doi.org/10.1021/acsami.1c01844

55. 3D-bioprinted BMSC-laden biomimetic multiphasic scaffolds for efficient repair of osteochondral defects in an osteoarthritic rat model

Author:: Yanzhi Liu; Liuqi Peng; Lingli Li; Cuishan Huang; Keda Shi; Xiangbo Meng; Pinpin Wang; Mingming Wu; Ling Li; Huijuan Cao; Kefeng Wu; Qingqiang Zeng; Haobo Pan; William Weijia Lu; Ling Qin; Changshun Ruan; Xinluan Wang
Source:: Biomaterials 2021 v.279 pp. 121216
ISSN:: 0142-9612
Subject:: animal models; biocompatible materials; biomimetics; cartilage; chondrogenesis; collagen; diclofenac; extracellular matrix; gait; hyaluronic acid; inflammation; mesenchymal stromal cells; osteoarthritis
Abstract:: ... Osteochondral defect repair in osteoarthritis (OA) remains an unsolved clinical problem due to the lack of enough seed cells in the defect and chronic inflammation in the joint. To address this clinical need, we designed a bone marrow-derived mesenchymal stem cell (BMSC)-laden 3D-bioprinted multilayer scaffold with methacrylated hyaluronic acid (MeHA)/polycaprolactone incorporating kartogenin and ...
DOI:: 10.1016/j.biomaterials.2021.121216
: https://doi.org/10.1016/j.biomaterials.2021.121216

56. 3D-printed oxygen-releasing scaffolds improve bone regeneration in mice

Author:: Ashley L. Farris; Dennis Lambrechts; Yuxiao Zhou; Nicholas Y. Zhang; Naboneeta Sarkar; Megan C. Moorer; Alexandra N. Rindone; Ethan L. Nyberg; Alexander Perdomo-Pantoja; S.J. Burris; Kendall Free; Timothy F. Witham; Ryan C. Riddle; Warren L. Grayson
Source:: Biomaterials 2022 v.280 pp. 121318
ISSN:: 0142-9612
Subject:: antioxidants; biocompatible materials; biodegradability; bone formation; bones; extracellular matrix; humans; hyperoxia; hypoxia; mice; osteopontin; oxygen; polyvinyl alcohol; therapeutics; tissue repair; toxicity
Abstract:: ... Low oxygen (O₂) diffusion into large tissue engineered scaffolds hinders the therapeutic efficacy of transplanted cells. To overcome this, we previously studied hollow, hyperbarically-loaded microtanks (μtanks) to serve as O₂ reservoirs. To adapt these for bone regeneration, we fabricated biodegradable μtanks from polyvinyl alcohol and poly (lactic-co-glycolic acid) and embedded them to form 3D-pr ...
DOI:: 10.1016/j.biomaterials.2021.121318
: https://doi.org/10.1016/j.biomaterials.2021.121318

57. 3DICE coding matrix multidirectional macro-architecture modulates cell organization, shape, and co-cultures endothelization network

Author:: Raphaël F. Canadas; João B. Costa; Zhengwei Mao; Changyou Gao; Utkan Demirci; Rui L. Reis; Alexandra P. Marques; Joaquim M. Oliveira
Source:: Biomaterials 2021 v.277 pp. 121112
ISSN:: 0142-9612
Subject:: anisotropy; biocompatible materials; biomarkers; biomechanics; coculture; cryogels; drugs; extracellular matrix; ice; isotropy; liver cirrhosis; medicine; osteopontin
Abstract:: ... Natural extracellular matrix governs cells providing biomechanical and biofunctional outstanding properties, despite being porous and mostly made of soft materials. Among organs, specific tissues present specialized macro-architectures. For instance, hepatic lobules present radial organization, while vascular sinusoids are branched from vertical veins, providing specific biofunctional features. Th ...
DOI:: 10.1016/j.biomaterials.2021.121112
: https://doi.org/10.1016/j.biomaterials.2021.121112

58. 4-Methylumbelliferone Inhibits Angiogenesis in Vitro and in Vivo

Author:: García-Vilas Javier A.; Quesada Ana R.; Medina Miguel Ángel
Source:: Journal of agricultural and food chemistry 2013 v.61 no.17 pp. 4063-4071
ISSN:: 1520-5118
Subject:: adhesion; angiogenesis; biosynthesis; cell proliferation; endothelial cells; extracellular matrix; gelatinase A; gene expression regulation; hyaluronic acid; inhibitory concentration 50; minimum inhibitory concentration
Abstract:: ... 4-Methylumbelliferone (4-MU) is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects. The objective of the present study was to determine the potential of 4-MU as an antiangiogenic compound. To fulfill this aim, cultured endothelial cells were used to perform an array of in vitro assays, as well as two different in vivo angiogenesis assays. This study demonstrates t ...
DOI:: 10.1021/jf303062h
PubMed:: 23581646
: https://doi.org/10.1021/jf303062h

59. 4. The promises of tissue engineering for organ building and banking

Author:: Karoly Jakab; Francoise Marga; Cyrille Norotte; Gabor Forgacs
Source:: Cryobiology 2015 v.71 no.1 pp. 165-166
ISSN:: 0011-2240
Subject:: bioprinting; bioreactors; extracellular matrix; longevity; morphogenesis; patients; tissue banks; tissue engineering; tissues
Abstract:: ... With aging population, increase in longevity and decrease in the number of qualified donors, the need to find alternative solutions to current organ replacement methods is rapidly becoming a critical issue. Tissue engineering has appeared as a potentially viable solution. Classical tissue engineering is based on culturing cells in scaffolds, artificial extracellular matrix mimics, with the hope th ...
DOI:: 10.1016/j.cryobiol.2015.05.010
: https://doi.org/10.1016/j.cryobiol.2015.05.010

60. 5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

Author:: Jolanta Opiela; Daniel Lipiński; Joanna Romanek; Wojciech Juzwa; Michał Bochenek; Piotr Wilczek
Source:: Annals of animal science 2016 v.16 no.2 pp. 369-385
ISSN:: 2300-8733
Subject:: adipocytes; adipogenesis; bone formation; extracellular matrix; flow cytometry; fluorescent antibody technique; gelatinase A; osteocalcin; polymerase chain reaction; stem cells; swine; transcription (genetics); transcription factors
Abstract:: ... Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) and its inhibitor TIMP-2, MEF2a and TAZ transcription factors are involved in porcine MSC differentiation towards adipocytes and osteocytes. Flow cytometry and immunofluorescence were used to investigate ...
DOI:: 10.1515/aoas-2015-0065
: https://doi.org/10.1515/aoas-2015-0065

61. 52 Decellularization of goat uterus as a promising 3-dimensional homing matrix of biological scaffold: A pilot study

Author:: M. Ghiringhelli; N. Verdile; T. A. L. Brevini; F. Gandolfi
Source:: Reproduction, fertility, and development 2019 v.31 no.1 pp. 151-152
ISSN:: 1031-3613
Subject:: DNA; analysis of variance; animal models; bioengineering; collagen; deoxycholic acid; dimethyl sulfoxide; extracellular matrix; female fertility; fluorescent antibody technique; goats; human population; medicine; opacity; patients; quantitative polymerase chain reaction; slaughter; uterus; veterinary medicine
Abstract:: ... Female infertility or absence of a functional uterus has a prevalence of 3 to 5% in the general human population. Nowadays most patients diagnosed with absolute uterine factor infertility remain untreatable. Moreover, the world population is aging rapidly, and as people live longer, uterus frailty increases the risk of developing age-related diseases. Whole-organ decellularization and recellulariz ...
DOI:: 10.1071/RDv31n1Ab52
: https://doi.org/10.1071/RDv31n1Ab52

62. 5α-Dihydrotestosterone enhances wound healing in diabetic rats

Author:: Reggiani V. Gonçalves; Rômulo D. Novaes; Mariáurea M. Sarandy; Eduardo M. Damasceno; Sérgio L.P. da Matta; Neire M. de Gouveia; Mariella B. Freitas; Foued S. Espindola
Source:: Life sciences 2016 v.152 pp. 67-75
ISSN:: 0024-3205
Subject:: animal disease models; antioxidants; collagen; diabetes; ethics; extracellular matrix; intraperitoneal injection; laboratory animals; lanolin; ointments; rats; silver; sodium chloride; streptozotocin; sulfadiazine; superoxide dismutase; testosterone; tissue repair; tissues
Abstract:: ... Wound healing involves a complex interaction between the cells, extracellular matrix and oxidative response.Analyze the effects of 5α-Dihydrotestosterone (5α-DTH) ointment in cutaneous wound healing by secondary intention in diabetic Wistar rats.Rats (302.23±26.23g, n=48) were maintained in cages with food and water ad libitum in accordance with the Guiding Principles in the Use of Animal Ethics C ...
DOI:: 10.1016/j.lfs.2016.03.019
: https://doi.org/10.1016/j.lfs.2016.03.019

63. 6-Month aortic valve implantation of an off-the-shelf tissue-engineered valve in sheep

Author:: Zeeshan Syedain; Jay Reimer; Jillian Schmidt; Matthew Lahti; James Berry; Richard Bianco; Robert T. Tranquillo
Source:: Biomaterials 2015 v.73 pp. 175-184
ISSN:: 0142-9612
Subject:: DNA; animal models; calcification; collagen; compliance; elastin; extracellular matrix; hemodynamics; histology; in vitro studies; mechanical properties; patients; prostheses; sheep; surgery; tissue engineering
Abstract:: ... Diseased aortic valves often require replacement, with over 30% of the current aortic valve surgeries performed in patients who will outlive a bioprosthetic valve. While many promising tissue-engineered valves have been created in the lab using the cell-seeded polymeric scaffold paradigm, none have been successfully tested long-term in the aortic position of a pre-clinical model. The high pressure ...
DOI:: 10.1016/j.biomaterials.2015.09.016
PubMed:: 26409002
PubMed Central:: PMC5520964
: https://doi.org/10.1016/j.biomaterials.2015.09.016

64. 7. An approach to molecular characteristic of collagen mesh extracellular matrix in processed tissue banking, by diffractive techniques

Author:: H. Pérez Campos; M. Saldias; G. Sanchez; P. Martucci; M. Acosta; R. Faccio; L. Suescun; M. Romero; A. Mombru
Source:: Cryobiology 2012 v.65 no.3 pp. 340-341
ISSN:: 0011-2240
Subject:: Raman spectroscopy; X-radiation; X-ray diffraction; amnion; aseptic conditions; banking; biopolymers; chemical residues; collagen; cryopreservation; extracellular matrix; freezing; humans; placenta; pregnancy; public health; sodium chloride; temperature; tissues; Uruguay
Abstract:: ... INTRODUCTION: Preservation techniques are of fundamental support for conservation of tissue banking (TB) biological materials. However, controversial views remain on the effects at the molecular level that preservation procedures could produce on the functional structures of tissues. Both programmed cryopreservation and glycerolized tissue preservation are common TB methods in technical preservati ...
DOI:: 10.1016/j.cryobiol.2012.07.005
: https://doi.org/10.1016/j.cryobiol.2012.07.005

65. HD-AGPs as Speciation Genes: Positive Selection on a Proline-Rich Domain in Non-Hybridizing Species of Petunia, Solanum, and Nicotiana

Author:: Tara D. Callaway; Anu Singh-Cundy
Source:: Plants 2019 v.8 no.7 pp. -
ISSN:: 2223-7747
Subject:: Nicotiana; Petunia axillaris; Petunia integrifolia; Solanum; environmental factors; extracellular matrix; genes; histidine; pistil; pollen tubes; pollinators; proteins; seed set; sympatric speciation
Abstract:: ... Transmitting tissue-specific proteins (TTS proteins) are abundant in the extracellular matrix of Nicotiana pistils, and vital for optimal pollen tube growth and seed set. We have identified orthologs from several species in the Solanaceae, including Petunia axillaris axillaris and Petunia integrifolia. We refer to TTS proteins and their orthologs as histidine domain-arabinogalactan proteins (HD-AG ...
DOI:: 10.3390/plants8070211
PubMed:: 31288469
PubMed Central:: PMC6681252
: https://doi.org/10.3390/plants8070211

66. Perilla frutescens Sprout Extract Protect Renal Mesangial Cell Dysfunction against High Glucose by Modulating AMPK and NADPH Oxidase Signaling

Author:: Ha-Rim Kim; Seon-Young Kim
Source:: Nutrients 2019 v.11 no.2 pp. -
ISSN:: 2072-6643
Subject:: NAD(P)H oxidase (H2O2-forming); Perilla frutescens; cell proliferation; collagen; diabetic nephropathy; extracellular matrix; fibronectins; fibrosis; glucose; hyperglycemia; hypertrophy; mice; reactive oxygen species
Abstract:: ... Perilla frutescens (L.) Britt. var. japonica (Hassk.) Hara (PF), is a medical herb of the Lamiaceae family. We have previously reported that the PF sprout extract (PFSE) is effective in treating hyperglycemia. However, the role of PFSE on glomerular mesangial cells (MCs) proliferation and the extracellular matrix (ECM) accumulation in a diabetic condition are still unclear. Therefore, in this stud ...
DOI:: 10.3390/nu11020356
PubMed:: 30744045
PubMed Central:: PMC6413074
: https://doi.org/10.3390/nu11020356

67. A 3-D in vitro co-culture model of mammary gland involution

Author:: Jonathan J. Campbell; Laur-Alexandru Botos; Timothy J. Sargeant; Natalia Davidenko; Ruth E. Cameron; Christine J. Watson
Source:: Integrative biology 2014 v.6 no.6 pp. 618-626
ISSN:: 1757-9708
Subject:: adipose tissue; autophagy; breast neoplasms; breasts; cell lines; coculture; collagen; cytokines; drugs; epithelial cells; epithelium; extracellular matrix; high-throughput screening methods; hyaluronic acid; macrophages; mammary glands; models; monocytes; phenotype; progeny; prolactin; signal transduction; therapeutics; tissue repair; weaning
Abstract:: ... Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring. These processes are mediated in part through Jak/Stat signalling pathways, which can be deregulated in breast cancer. Synthetic in vitro analogues of the breast cou ...
DOI:: 10.1039/c3ib40257f
: https://doi.org/10.1039/c3ib40257f

68. A 32-Kilodalton Hydrolase Plays an Important Role in Paracoccidioides brasiliensis Adherence to Host Cells and Influences Pathogenicity

Author:: Hernández, Orville; Almeida, Agostinho J.; Gonzalez, Angel; Garcia, Ana Maria; Tamayo, Diana; Cano, Luz Elena; Restrepo, Angela; McEwen, Juan G.
Source:: Infection and immunity 2010 v.78 no.12 pp. 5280-5286
ISSN:: 0019-9567
Subject:: Paracoccidioides brasiliensis; animal models; antisense RNA; epithelial cells; extracellular matrix; gene expression; host-pathogen relationships; humans; hydrolases; immune response; pathogens; phylogeny; proteins; sequence analysis; viability; virulence; yeasts
Abstract:: ... One of the most crucial events during infection with the dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in the establishment of disease. In this study, we have evaluated the relevance of a 32-kDa protein, a putative adhesion member of the haloacid dehalogenase (HAD) superfamily of hydrolases, in the virulence of this fungus. Protein sequenc ...
DOI:: 10.1128/IAI.00692-10
PubMed:: 20876288
PubMed Central:: PMC2981316
: https://doi.org/10.1128/IAI.00692-10

69. A 3D bioinspired highly porous polymeric scaffolding system for in vitro simulation of pancreatic ductal adenocarcinoma

Author:: Stella Totti; Mark C. Allenby; Susana Brito Dos Santos; Athanasios Mantalaris; Eirini G. Velliou
Source:: RSC advances 2018 v.8 no.37 pp. 20928-20940
ISSN:: 2046-2069
Subject:: adenocarcinoma; apoptosis; biomarkers; drugs; extracellular matrix; fibronectins; hypoxia-inducible factor 1; image analysis; in vivo studies; neoplasm cells; niches; pancreatic neoplasms; polyurethanes; prognosis; screening; survival rate; tissue engineering
Abstract:: ... Pancreatic ductal adenocarcinoma is an aggressive disease with an extremely low survival rate. This is due to the (i) poor prognosis and (ii) high resistance of the disease to current treatment options. The latter is partly due to the very complex and dense tissue/tumour microenvironment of pancreatic cancer, which contributes to the disease's progression and the inhibition of apoptotic pathways. ...
DOI:: 10.1039/c8ra02633e
: https://doi.org/10.1039/c8ra02633e

70. A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss

Author:: Yeong-Jin Choi; Young-Joon Jun; Dong Yeon Kim; Hee-Gyeong Yi; Su-Hun Chae; Junsu Kang; Juyong Lee; Ge Gao; Jeong-Sik Kong; Jinah Jang; Wan Kyun Chung; Jong-Won Rhie; Dong-Woo Cho
Source:: Biomaterials 2019 v.206 pp. 160-169
ISSN:: 0142-9612
Subject:: animal models; biomimetics; bioprinting; cell viability; extracellular matrix; hypoxia; innervation; muscles
Abstract:: ... Volumetric muscle loss (VML) is an irrecoverable injury associated with muscle loss greater than 20%. Although hydrogel-based 3D engineered muscles and the decellularized extracellular matrix (dECM) have been considered for VML treatment, they have shown limited efficacy. We established a novel VML treatment with dECM bioink using 3D cell printing technology. Volumetric muscle constructs composed ...
DOI:: 10.1016/j.biomaterials.2019.03.036
: https://doi.org/10.1016/j.biomaterials.2019.03.036

71. A 3D matrix platform for the rapid generation of therapeutic anti-human carcinoma monoclonal antibodies

Author:: David T. Dudley; Xiao-Yan Li; Casey Y. Hu; Celina G. Kleer; Amanda L. Willis; Stephen J. Weiss
Source:: Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.41 pp. 14882-14887
ISSN:: 0027-8424
Subject:: antigens; antineoplastic activity; breast neoplasms; carcinoma; cell growth; collagen; humans; integrins; monoclonal antibodies; neoplasm cells; patients; tissues
Abstract:: ... Efforts to develop unbiased screens for identifying novel function-blocking monoclonal antibodies (mAbs) in human carcinomatous states have been hampered by the limited ability to design in vitro models that recapitulate tumor cell behavior in vivo. Given that only invasive carcinoma cells gain permanent access to type I collagen-rich interstitial tissues, an experimental platform was established ...
DOI:: 10.1073/pnas.1410996111
PubMed:: 25267635
PubMed Central:: PMC4205646
: https://doi.org/10.1073/pnas.1410996111

72. A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate®

Author:: Kristin M. Bircsak; Richard DeBiasio; Mark Miedel; Alaa Alsebahi; Ryan Reddinger; Anthony Saleh; Tongying Shun; Lawrence A. Vernetti; Albert Gough
Source:: Toxicology 2021 v.450 pp. 152667
ISSN:: 0300-483X
Subject:: albumins; automation; cell viability; coculture; dosage; dose response; endothelial cells; extracellular matrix; hepatocytes; hepatotoxicity; high-throughput screening methods; laboratories; liquids; liver; macrophages; models; organ-on-a-chip; prioritization; protocols; screening; secretion; staining; toxicology; urea; viability assays
Abstract:: ... We report the development, automation and validation of a 3D, microfluidic liver-on-a-chip for high throughput hepatotoxicity screening, the OrganoPlate LiverTox™. The model is comprised of aggregates of induced pluripotent stem cell (iPSC)-derived hepatocytes (iHep) seeded in an extracellular matrix in the organ channel and co-cultured with endothelial cells and THP-1 monoblasts differentiated to ...
DOI:: 10.1016/j.tox.2020.152667
: https://doi.org/10.1016/j.tox.2020.152667

73. A 3D tension bioreactor platform to study the interplay between ECM stiffness and tumor phenotype

Author:: Luke Cassereau; Yekaterina A. Miroshnikova; Guanqing Ou; Johnathon Lakins; Valerie M. Weaver
Source:: Journal of biotechnology 2015 v.193 pp. 66-69
ISSN:: 0168-1656
Subject:: mechanics; topography; phenotype; cardiovascular diseases; neoplasms; porosity; bioreactors; epithelial cells; hydrocolloids; collagen; neoplasm cells; mammary glands; hyaluronic acid; extracellular matrix
Abstract:: ... Extracellular matrix (ECM) structure, composition, and stiffness have profound effects on tissue development and pathologies such as cardiovascular disease and cancer. Accordingly, a variety of synthetic hydrogel systems have been designed to study the impact of ECM composition, density, mechanics, and topography on cell and tissue phenotype. However, these synthetic systems fail to accurately rec ...
DOI:: 10.1016/j.jbiotec.2014.11.008
PubMed:: 25435379
PubMed Central:: PMC4454500
: https://doi.org/10.1016/j.jbiotec.2014.11.008

74. A 3D tissue model-on-a-chip for studying the effects of human senescent fibroblasts on blood vessels

Author:: Joris Pauty; Shizuka Nakano; Ryo Usuba; Tadaaki Nakajima; Yoshikazu Johmura; Satotaka Omori; Naoya Sakamoto; Akihiko Kikuchi; Makoto Nakanishi; Yukiko T. Matsunaga
Source:: Biomaterials science 2021 v.9 no.1 pp. 199-211
ISSN:: 2047-4849
Subject:: angiogenesis; biocompatible materials; blood; cell senescence; collagen; extracellular matrix; fibroblasts; gels; humans; longevity; microvessels; models; phenotype
Abstract:: ... All human tissues experience aging that eventually causes organ dysfunction and disease. Cellular senescence was discovered in fibroblasts cultured in vitro. In adults, it is a primary defense mechanism against cancer, but also a major contributor to lifespan limits and disorders associated with aging. To assess how human blood vessels change in an aged environment, we developed an elementary tiss ...
DOI:: 10.1039/d0bm01297a
: https://doi.org/10.1039/d0bm01297a

75. A Biomimetic Macroporous Hybrid Scaffold with Sustained Drug Delivery for Enhanced Bone Regeneration

Author:: Seunghun S. Lee; Matthias Santschi; Stephen J. Ferguson
Source:: Biomacromolecules 2021 v.22 no.6 pp. 2460-2471
ISSN:: 1526-4602
Subject:: alizarin; apatite; biocompatibility; biomimetics; body fluids; bone formation; bone morphogenetic proteins; cell proliferation; cryogels; drugs; extracellular matrix; gelatin; heparin; hydrophilicity; hydrophobicity; porous media; quantitative polymerase chain reaction; strength (mechanics)
Abstract:: ... Bone regeneration is a highly complex physiological process regulated by several factors. In particular, bone-mimicking extracellular matrix and available osteogenic growth factors such as bone morphogenetic protein (BMP) have been regarded as key contributors for bone regeneration. In this study, we developed a biomimetic hybrid scaffold (CEGH) with sustained release of BMP-2 that would result in ...
DOI:: 10.1021/acs.biomac.1c00241
: https://doi.org/10.1021/acs.biomac.1c00241

76. A Citrus Extract Containing Flavanones Represses Plasminogen Activator Inhibitor-1 (PAI-1) Expression and Regulates Multiple Inflammatory, Tissue Repair, and Fibrosis Genes in Human Colon Fibroblasts

Author:: Gimenez-Bastida, Juan A.; Martinez-Florensa, Mario; Espin, Juan-Carlos; Tomas-Barberan, Francisco A.; Garcia-Conesa, Maria-Teresa
Source:: Journal of agricultural and food chemistry 2009 v.57 no.19 pp. 9305-9315
ISSN:: 0021-8561
Subject:: anti-inflammatory activity; metalloproteinases; plant extracts; cell adhesion; extracellular matrix; flavanones; tissue repair; fibrosis; gene expression; gene expression regulation; colon; transcription factors; human cell lines; messenger RNA; tumor necrosis factor-alpha; in vitro studies; monocytes; inflammation; oranges; plasminogen activator; fibroblasts
Abstract:: ... The consumption of flavonoid-rich Citrus extracts has been associated with multiple beneficial effects including anti-inflammatory properties, but the potential effects on the inflammatory responses in the gut have not been thoroughly investigated. We used microarrays to search for molecular changes induced in human colon fibroblasts in response to the exposure to a flavanone-rich bitter orange ex ...
DOI:: 10.1021/jf901983g
PubMed:: 19728713
: https://doi.org/10.1021/jf901983g

77. A Clp/Hsp100 Chaperone Functions in Myxococcus xanthus Sporulation and Self-Organization

Author:: Jinyuan Yan; Anthony G. Garza; Michael D. Bradley; Roy D. Welch
Source:: Journal of bacteriology 2012 v.194 no.7 pp. 1689-1696
ISSN:: 0021-9193
Subject:: Myxococcus xanthus; bacteria; cell movement; extracellular matrix; genes; loci; molecular chaperones; phenotype; pleiotropy; protein degradation; sporulation
Abstract:: ... The Clp/Hsp100 proteins are chaperones that play a role in protein degradation and reactivation. In bacteria, they exhibit a high degree of pleiotropy, affecting both individual and multicellular phenotypes. In this article, we present the first characterization of a Clp/Hsp100 homolog in Myxococcus xanthus (MXAN_4832 gene locus). Deletion of MXAN_4832 causes defects in both swarming and aggregati ...
DOI:: 10.1128/JB.06492-11
PubMed:: 22287524
PubMed Central:: PMC3302451
: https://doi.org/10.1128/JB.06492-11

78. A Combination of Glycerol and Manganese Promotes Biofilm Formation in Bacillus subtilis via Histidine Kinase KinD Signaling

Author:: Moshe Shemesh; Yunrong Chai
Source:: Journal of bacteriology 2013 v.195 no.12 pp. 2747-2754
ISSN:: 0021-9193
Subject:: Bacillus cereus; Bacillus subtilis; biofilm; extracellular matrix; glycerol; histidine kinase; manganese; operon; phosphorylation; spore-forming bacteria; sporulation; transcription (genetics); transcription factors
Abstract:: ... The spore-forming bacterium Bacillus subtilis forms matrix-enclosed biofilms in response to environmental cues that to date remain poorly defined. Biofilm formation depends on the synthesis of an extracellular matrix, which is indirectly regulated by the transcriptional regulator Spo0A. The activity of Spo0A depends on its phosphorylation state. The level of phosphorylated Spo0A (Spo0A∼P) is contr ...
DOI:: 10.1128/JB.00028-13
PubMed:: 23564171
PubMed Central:: PMC3697245
: https://doi.org/10.1128/JB.00028-13

79. A Comparison of Scaffold-free and Scaffold-based Reconstructed Human Skin Models as Alternatives to Animal Use

Author:: Beste Kinikoglu
Source:: ATLA. Alternatives to laboratory animals v.45 no.6 pp. 309-316
ISSN:: 0261-1929
Subject:: artificial skin; basement membrane; collagen; cosmetics; extracellular matrix; fibroblasts; immunohistochemistry; laboratories; models; polymers
Abstract:: ... Tissue engineered full-thickness human skin substitutes have various applications in the clinic and in the laboratory, such as in the treatment of burns or deep skin defects, and as reconstructed human skin models in the safety testing of drugs and cosmetics and in the fundamental study of skin biology and pathology. So far, different approaches have been proposed for the generation of reconstruct ...
DOI:: 10.1177/026119291704500607
: https://doi.org/10.1177/026119291704500607

80. A Core Subunit of the RNA-Processing/Degrading Exosome Specifically Influences Cuticular Wax Biosynthesis in Arabidopsis

Author:: Hooker, Tanya S.; Lam, Patricia; Zheng, Huanquan; Kunst, Ljerka
Source:: plant cell 2007 v.19 no.3 pp. 904-913
ISSN:: 1040-4651
Subject:: Arabidopsis thaliana; biosynthesis; cutin; epicuticular wax; extracellular matrix; genes; messenger RNA; plant organs; polyesters; proteins; ribonucleases; yeasts
Abstract:: ... The cuticle is an extracellular matrix composed of cutin polyester and waxes that covers aerial organs of land plants and protects them from environmental stresses. The Arabidopsis thaliana cer7 mutant exhibits reduced cuticular wax accumulation and contains considerably lower transcript levels of ECERIFERUM3/WAX2/YORE-YORE (CER3/WAX2/YRE), a key wax biosynthetic gene. We show here that CER7 prote ...
DOI:: 10.1105/tpc.106.049304
PubMed:: 17351114
PubMed Central:: PMC1867365
: https://doi.org/10.1105/tpc.106.049304

81. A DOPA-functionalized chondroitin sulfate-based adhesive hydrogel as a promising multi-functional bioadhesive

Author:: Wenzhen Zhu; Jabed Iqbal; Dong-An Wang
Source:: Journal of materials chemistry B 2019 v.7 no.10 pp. 1741-1752
ISSN:: 2050-7518
Subject:: animals; bioadhesives; biocompatibility; cartilage; chondroitin sulfate; dietary supplements; extracellular matrix; hydrogels; pharmaceutical industry; tissue repair; water content
Abstract:: ... Great progress has been achieved on the study of hydrogels, which were presented for the first time in 1960 by Otto Wichterle and Drahoslav Lím. The two crucial properties of hydrogels, namely high water content and biocompatibility, have made hydrogels ideal compositions in the development of bioadhesives in recent years. Chondroitin sulfate (CS), a sulfated glycosaminoglycan (GAG), is distribute ...
DOI:: 10.1039/c8tb01990h
: https://doi.org/10.1039/c8tb01990h

82. A Dictyostelium cellobiohydrolase orthologue that affects developmental timing

Author:: Mizuho Kunii; Mami Yasuno; Yuki Shindo; Takefumi Kawata
Source:: Development genes and evolution 2014 v.224 no.1 pp. 25-35
ISSN:: 0949-944X
Subject:: Dictyostelium discoideum; cellulose; cellulose 1,4-beta-cellobiosidase; culture media; endo-1,4-beta-glucanase; extracellular matrix; fruiting bodies; gel electrophoresis; genes; mutants; spores
Abstract:: ... Dictyostelium discoideum is a facultative multicellular amoebozoan with cellulose in the stalk and spore coat of its fruiting body as well as in the extracellular matrix of the migrating slug. The organism also harbors a number of cellulase genes. One of them, cbhA, was identified as a candidate cellobiohydrolase gene based on the strong homology of its predicted protein product to fungal cellobio ...
DOI:: 10.1007/s00427-013-0460-x
PubMed:: 24240571
: https://doi.org/10.1007/s00427-013-0460-x

83. A Diels–Alder modulated approach to control and sustain the release of dexamethasone and induce osteogenic differentiation of human mesenchymal stem cells

Author:: Kenneth C. Koehler; Daniel L. Alge; Kristi S. Anseth; Christopher N. Bowman
Source:: Biomaterials 2013 v.34 no.16 pp. 4150-4158
ISSN:: 0142-9612
Subject:: alkaline phosphatase; bone formation; dexamethasone; ethylene glycol; extracellular matrix; humans; hydrocolloids; mineralization; models; molecular weight; stem cells
Abstract:: ... We report a new approach to controlled drug release based upon exploiting the dynamic equilibrium that exists between Diels–Alder reactants and products, demonstrating the release of a furan containing dexamethasone peptide (dex-KGPQG-furan) from a maleimide containing hydrogel. Using a reaction-diffusion model, the release kinetics were tuned to achieve sustained concentrations conducive to osteo ...
DOI:: 10.1016/j.biomaterials.2013.02.020
PubMed:: 23465826
PubMed Central:: PMC3604741
: https://doi.org/10.1016/j.biomaterials.2013.02.020

84. A Distinguishable Role of eDNA in the Viscoelastic Relaxation of Biofilms

Author:: Brandon W. Peterson; Henny C. van der Mei; Jelmer Sjollema; Henk J. Busscher; Prashant K. Sharma
Source:: mBio 2013 v.4 no.5 pp. e00497-13
ISSN:: 2150-7511
Subject:: DNA; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; Streptococcus mutans; bacteria; biofilm; chemical composition; deformation; extracellular matrix; models; polysaccharides; proteins; stress relaxation; viscoelasticity
Abstract:: ... Bacteria in the biofilm mode of growth are protected against chemical and mechanical stresses. Biofilms are composed, for the most part, of extracellular polymeric substances (EPSs). The extracellular matrix is composed of different chemical constituents, such as proteins, polysaccharides, and extracellular DNA (eDNA). Here we aimed to identify the roles of different matrix constituents in the vis ...
DOI:: 10.1128/mBio.00497-13
PubMed:: 24129256
PubMed Central:: PMC3812712
: https://doi.org/10.1128/mBio.00497-13

85. A Dual Network Hydrogel Sunscreen Based on Poly-γ-glutamic Acid/Tannic Acid Demonstrates Excellent Anti-UV, Self-Recovery, and Skin-Integration Capacities

Author:: Rui Wang; Xuexue Wang; Yijing Zhan; Zheng Xu; Zongqi Xu; Xiaohai Feng; Sha Li; Hong Xu
Source:: ACS applied materials & interfaces 2019 v.11 no.41 pp. 37502-37512
ISSN:: 1944-8252
Subject:: UV filters; bioadhesives; extracellular matrix; hydrogels; hydrogen bonding; microstructure; polyglutamic acid; sunscreens; tannins
Abstract:: ... Novel sunscreen products based on bioadhesive/gel systems that can prevent the skin penetration behaviors of UV filters have attracted increasing attention in recent years. However, integration is very difficult to achieve and control on the wet surface of the skin under sweaty/dynamic physiological conditions, resulting in functional failure. Herein, we demonstrated the fabrication of a novel dua ...
DOI:: 10.1021/acsami.9b14538
: https://doi.org/10.1021/acsami.9b14538

86. A Dual SILAC Proteomic Labeling Strategy for Quantifying Constitutive and Cell–Cell Induced Protein Secretion

Author:: Stiess Michael; Wegehingel Sabine; Nguyen Chuong; Nickel Walter; Bradke Frank; Cambridge Sidney B.
Source:: Journal of Proteome Research 2015 v.14 no.8 pp. 3229-3238
ISSN:: 1535-3907
Subject:: blood serum; cerebellum; neurons; protein secretion; proteins; proteome; proteomics; tissues
Abstract:: ... Recent evidence suggests that the extracellular protein milieu is much more complex than previously assumed as various secretome analyses from different cell types described the release of hundreds to thousands of proteins. The extracellular function of many of these proteins has yet to be determined particularly in the context of three-dimensional tissues with abundant cell–cell contacts. Toward ...
DOI:: 10.1021/acs.jproteome.5b00199
: https://doi.org/10.1021/acs.jproteome.5b00199

87. A Dual-Responsive Peptide-Based Smart Biointerface with Biomimetic Adhesive Behaviors for Bacterial Isolation

Author:: Fenghua Wang; Xiangyu Sha; Xiaolu Song; Mengqi Bai; Xiaohua Tian; Lei Liu
Source:: Langmuir 2021 v.37 no.50 pp. 14638-14645
ISSN:: 1520-5827
Subject:: adhesion; biocompatible materials; biomimetics; chemical bonding; enzymes; extracellular matrix; medicine; peptides; sugars
Abstract:: ... As mimics of the extracellular matrix, surfaces with the capability of capturing and releasing specific cells in a smart and controllable way play an important role in bacterial isolation. In this work, we fabricated a dual-responsive smart biointerface via peptide self-assembly and reversible covalent chemistry biomimetic adhesion behavior for bacterial isolation. Compared with that of the bioint ...
DOI:: 10.1021/acs.langmuir.1c02357
: https://doi.org/10.1021/acs.langmuir.1c02357

88. A General Approach for Generating Fluorescent Probes to Visualize Piconewton Forces at the Cell Surface

Author:: Chang Yuan; Liu Zheng; Zhang Yun; Galior Kornelia; Yang Jeffery; Salaita Khalid
Source:: Journal of the American Chemical Society 2016 v.138 no.9 pp. 2901-2904
ISSN:: 1520-5126
Subject:: extracellular matrix; fluorescence; fluorescent dyes; image analysis; integrins; ligands; mechanotransduction; receptors; temporal variation
Abstract:: ... Mechanical forces between cells and their extracellular matrix (ECM) are mediated by dozens of different receptors. These biophysical interactions play fundamental roles in processes ranging from cellular development to tumor progression. However, mapping the spatial and temporal dynamics of tension among various receptor–ligand pairs remains a significant challenge. To address this issue, we have ...
DOI:: 10.1021/jacs.5b11602
PubMed:: 26871302
PubMed Central:: PMC5589465
: https://doi.org/10.1021/jacs.5b11602

89. A High-Throughput Assay for Collagen Secretion Suggests an Unanticipated Role for Hsp90 in Collagen Production

Author:: Madeline Y. Wong; Ngoc Duc Doan; Andrew S. DiChiara; Louis J. Papa; Jaime H. Cheah; Christian K. Soule; Nicki Watson; John D. Hulleman; Matthew D. Shoulders
Source:: Biochemistry 2018 v.57 no.19 pp. 2814-2827
ISSN:: 1520-4995
Subject:: biosynthesis; bone formation; cell lines; collagen; endoplasmic reticulum; extracellular matrix; fibrosis; genetic disorders; humans; luminescence; models; neoplasms; secretion; therapeutics; type I secretion system
Abstract:: ... Collagen overproduction is a feature of fibrosis and cancer, while insufficient deposition of functional collagen molecules and/or the secretion of malformed collagen is common in genetic disorders like osteogenesis imperfecta. Collagen secretion is an appealing therapeutic target in these and other diseases, as secretion directly connects intracellular biosynthesis to collagen deposition and biol ...
DOI:: 10.1021/acs.biochem.8b00378
PubMed:: 29676157
PubMed Central:: PMC6231715
: https://doi.org/10.1021/acs.biochem.8b00378

90. A Jonah-like chymotrypsin from the therapeutic maggot Lucilia sericata plays a role in wound debridement and coagulation

Author:: Anne-Kathrin Pöppel; Mareike Kahl; Andre Baumann; Jochen Wiesner; Anke Gökçen; Annika Beckert; Klaus T. Preissner; Andreas Vilcinskas; Zdeněk Franta
Source:: Insect biochemistry and molecular biology 2016 v.70 pp. 138-147
ISSN:: 0965-1748
Subject:: Drosophila melanogaster; Escherichia coli; Lucilia cuprina; Lucilia sericata; casein; chymotrypsin; coagulation; collagen; debridement; disinfection; drugs; excretion; extracellular matrix; fibronectins; humans; insect larvae; laminin; pH; temperature; tissue repair
Abstract:: ... Lucilia sericata larvae are used in maggot debridement therapy, a traditional wound healing approach that has recently been approved for the treatment of chronic wounds. Maggot excretion products (MEP) contain many different proteases that promote disinfection, debridement and the acceleration of wound healing, e.g. by activating the host contact phase/intrinsic pathway of coagulation. In order to ...
DOI:: 10.1016/j.ibmb.2015.11.012
: https://doi.org/10.1016/j.ibmb.2015.11.012

91. A Layer-by-Layer Single-Cell Coating Technique To Produce Injectable Beating Mini Heart Tissues via Microfluidics

Author:: Luis P. B. Guerzoni; Yoshinari Tsukamoto; David B. Gehlen; Dirk Rommel; Tamás Haraszti; Mitsuru Akashi; Laura De Laporte
Source:: Biomacromolecules 2019 v.20 no.10 pp. 3746-3754
ISSN:: 1526-4602
Subject:: cardiomyocytes; cell transplantation; cell viability; coatings; coculture; embryonic stem cells; encapsulation; extracellular matrix; fibroblasts; induced pluripotent stem cells; infarction; microfluidic technology; microgels; peptides; polyethylene glycol; tissue repair
Abstract:: ... Human induced pluripotent stem cells (hiPSCs) are used as an alternative for human embryonic stem cells. Cardiomyocytes derived from hiPSCs are employed in cardiac tissue regeneration constructs due to the heart’s low regeneration capacity after infarction. A coculture of hiPSC-CM and primary dermal fibroblasts is encapsulated in injectable poly(ethylene glycol)-based microgels via microfluidics t ...
DOI:: 10.1021/acs.biomac.9b00786
: https://doi.org/10.1021/acs.biomac.9b00786

92. A Layered View on Focal Adhesions

Author:: Karin Legerstee; Adriaan B. Houtsmuller
Source:: Biology 2021 v.10 no.11 pp. -
ISSN:: 2079-7737
Subject:: actin; cell movement; cytoskeleton; extracellular matrix; fluorescence; integrins; physiological transport; plasma membrane; talin
Abstract:: ... The cytoskeleton provides structure to cells and supports intracellular transport. Actin fibres are crucial to both functions. Focal Adhesions (FAs) are large macromolecular multiprotein assemblies at the ends of specialised actin fibres linking these to the extracellular matrix. FAs translate forces on actin fibres into forces contributing to cell migration. This review will discuss recent insigh ...
DOI:: 10.3390/biology10111189
: https://doi.org/10.3390/biology10111189

93. A Lymphocyte Serine Protease Granzyme A Causes Detachment of a Small-Intestinal Epithelial Cell Line (IEC-6)

Author:: Hirofumi HIRAYASU; Yumiko YOSHIKAWA; Satoshi TSUZUKI; Tohru FUSHIKI
Source:: Bioscience, biotechnology, and biochemistry 2008 v.72 no.9 pp. 2294-2302
ISSN:: 1347-6947
Subject:: adhesion; apoptosis; biotechnology; cell lines; collagen; cytotoxicity; deformation; epithelial cells; extracellular matrix; fibronectins; laminin; rats; serine proteinases; small intestine
Abstract:: ... Granzyme A (GzmA) is a serine protease (trypsin-like specificity) produced in cytotoxic lymphocytes. This enzyme is believed to enter virus-infected cells and growing tumors and induce apoptosis, but the roles of GzmA expressed in lymphocytes scattered through the epithelial layer of the normal small intestine are unknown. In the present study, recombinant rat GzmA (rGzmA) was found to cause morph ...
DOI:: 10.1271/bbb.80140
: https://doi.org/10.1271/bbb.80140

94. A LysR-Type Transcriptional Regulator in Burkholderia cenocepacia Influences Colony Morphology and Virulence

Author:: Bernier, Steve P.; Nguyen, David T.; Sokol, Pamela A.
Source:: Infection and immunity 2008 v.76 no.1 pp. 38-47
ISSN:: 0019-9567
Subject:: Burkholderia cepacia complex; agar; alfalfa; biofilm; biomass; extracellular matrix; genes; histopathology; models; mutagenesis; mutants; open reading frames; phenotype; seedlings; transcription factors; transmission electron microscopy; transposons; virulence
Abstract:: ... Burkholderia cenocepacia strain K56-2 typically has rough colony morphology on agar medium; however, shiny colony variants (shv) can appear spontaneously. These shv all had a minimum of 50% reduction in biomass formation and were generally avirulent in an alfalfa seedling infection model. Three shv--K56-2 S15, K56-2 S76, and K56-2 S86--were analyzed for virulence in a chronic agar bead model of re ...
DOI:: 10.1128/IAI.00874-07
PubMed:: 17967860
PubMed Central:: PMC2223663
: https://doi.org/10.1128/IAI.00874-07

95. A Marine λ-Oligocarrageenan Inhibits Migratory and Invasive Ability of MDA-MB-231 Human Breast Cancer Cells through Actions on Heparanase Metabolism and MMP-14/MMP-2 Axis

Author:: Rémi Cousin; Hugo Groult; Chanez Manseur; Romain Ferru-Clément; Mario Gani; Rachel Havret; Claire Toucheteau; Grégoire Prunier; Béatrice Colin; Franck Morel; Jean-Marie Piot; Isabelle Lanneluc; Kévin Baranger; Thierry Maugard; Ingrid Fruitier-Arnaudin
Source:: Marine drugs 2021 v.19 no.10 pp. -
ISSN:: 1660-3397
Subject:: breast neoplasms; cell movement; cell viability; cytotoxicity; enzyme activity; extracellular matrix; heparan sulfate; heparin; humans; metabolism; migratory behavior; oligosaccharides; pathogenesis
Abstract:: ... Sugar-based molecules such as heparins or natural heparan sulfate polysaccharides have been developed and widely studied for controlling heparanase (HPSE) enzymatic activity, a key player in extracellular matrix remodelling during cancer pathogenesis. However, non-enzymatic functions of HPSE have also been described in tumour mechanisms. Given their versatile properties, we hypothesized that sugar ...
DOI:: 10.3390/md19100546
: https://doi.org/10.3390/md19100546

96. A Marine-Derived Anti-Inflammatory Scaffold for Accelerating Skin Repair in Diabetic Mice

Author:: Xiaoli Huang; Na Guan; Qiu Li
Source:: Marine drugs 2021 v.19 no.9 pp. -
ISSN:: 1660-3397
Subject:: angiogenesis; biocompatible materials; extracellular matrix; inflammation; polysaccharides; polyvinyl alcohol; tissue repair
Abstract:: ... Reconstructing the typical analogue of extracellular matrix (ECM) in engineered biomaterials is essential for promoting tissue repair. Here, we report an ECM-mimetic scaffold that successfully accelerated wound healing through enhancing vascularization and regulating inflammation. We prepared an electrospun fiber comprising a brown alga-derived polysaccharide (BAP) and polyvinyl alcohol (PVA). The ...
DOI:: 10.3390/md19090496
: https://doi.org/10.3390/md19090496

97. A Mechanical Checkpoint Controls Multicellular Growth through YAP/TAZ Regulation by Actin-Processing Factors

Author:: Mariaceleste Aragona; Tito Panciera; Andrea Manfrin; Stefano Giulitti; Federica Michielin; Nicola Elvassore; Sirio Dupont; Stefano Piccolo
Source:: Cell 2013 v.154 pp. 1047-1059
ISSN:: 0092-8674
Subject:: extracellular matrix; mechanical stress; proteins; tissues
Abstract:: ... Key cellular decisions, such as proliferation or growth arrest, typically occur at spatially defined locations within tissues. Loss of this spatial control is a hallmark of many diseases, including cancer. Yet, how these patterns are established is incompletely understood. Here, we report that physical and architectural features of a multicellular sheet inform cells about their proliferative capac ...
DOI:: 10.1016/j.cell.2013.07.042
: https://doi.org/10.1016/j.cell.2013.07.042

98. A Mechanism for Localized Lignin Deposition in the Endodermis

Author:: Yuree Lee; Maria C. Rubio; Julien Alassimone; Niko Geldner
Source:: Cell 2013 v.153 pp. 402-412
ISSN:: 0092-8674
Subject:: Arabidopsis; NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); cell wall components; cell walls; cells; endodermis; extracellular matrix; lignin; models; peroxidases; polymerization; proteins; reactive oxygen species
Abstract:: ... The precise localization of extracellular matrix and cell wall components is of critical importance for multicellular organisms. Lignin is a major cell wall modification that often forms intricate subcellular patterns that are central to cellular function. Yet the mechanisms of lignin polymerization and the subcellular precision of its formation remain enigmatic. Here, we show that the Casparian s ...
DOI:: 10.1016/j.cell.2013.02.045
: https://doi.org/10.1016/j.cell.2013.02.045

99. A Mn(II)–Mn(II) center in human prolidase

Author:: Roberta Besio; Maria Camilla Baratto; Roberta Gioia; Enrico Monzani; Stefania Nicolis; Lucia Cucca; Antonella Profumo; Luigi Casella; Riccardo Basosi; Ruggero Tenni; Antonio Rossi; Antonella Forlino
Source:: BBA - Proteins and Proteomics 2013 v.1834 pp. 197-204
ISSN:: 1570-9639
Subject:: Escherichia coli; active sites; carcinogenesis; catalytic activity; denaturation; extracellular matrix; fluorescence emission spectroscopy; genes; humans; hydrolysis; imino acids; manganese; mutation; proline dipeptidase; protein folding; protein metabolism; proteolysis
Abstract:: ... Human prolidase, the enzyme responsible for the hydrolysis of the Xaa-Pro/Hyp peptide bonds, is a key player in the recycling of imino acids during the final stage of protein catabolism and extracellular matrix remodeling. Its metal active site composition corresponding to the maximal catalytic activity is still unknown, although prolidase function is of increasing interest due to the link with ca ...
DOI:: 10.1016/j.bbapap.2012.09.008
: https://doi.org/10.1016/j.bbapap.2012.09.008

100. A Molecular Clutch Disables Flagella in the Bacillus subtilis Biofilm

Author:: Blair, Kris M.; Turner, Linda; Winkelman, Jared T.; Berg, Howard C.; Kearns, Daniel B.
Source:: Science 2008 v.320 no.5883 pp. 1636-1638
ISSN:: 0036-8075
Subject:: Bacillus subtilis; bacteria; biofilm; biosynthesis; drug resistance; extracellular matrix; flagellum; operon
Abstract:: ... Biofilms are multicellular aggregates of sessile bacteria encased by an extracellular matrix and are important medically as a source of drug-resistant microbes. In Bacillus subtilis, we found that an operon required for biofilm matrix biosynthesis also encoded an inhibitor of motility, EpsE. EpsE arrested flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-genera ...
DOI:: 10.1126/science.1157877
: https://doi.org/10.1126/science.1157877

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1. (+)-Terpinen-4-ol Inhibits Bacillus cereus Biofilm Formation by Upregulating the Interspecies Quorum Sensing Signals Diketopiperazines and Diffusing Signaling Factors

2. (±)-Aspongamide A, an N-Acetyldopamine Trimer Isolated from the Insect Aspongopus chinensis, Is an Inhibitor of p-Smad3

3. (−)-Epigallocatechin-3-gallate inhibits matrix metalloproteinases in oral ulcers

4. (−)-Loliolide Isolated from Sargassum horneri Abate UVB-Induced Oxidative Damage in Human Dermal Fibroblasts and Subside ECM Degradation

5. 032 Cryobanking of testicular tissue in men of reproductive age

6. 1, 25-Dihydroxyvitamin D3 Altered Metabolism of MCF10A-ras Cells Decreases Viability in Extracellular Matrix Detached Conditions (FS13-05-19)

7. 111 Cryopreservation of stem cells inside alginate 3D constructs after high-voltage encapsulation

8. 116 In Vitro Transcriptomic Response of Bovine Oviduct Epithelial Cells to Direct or Indirect Embryo Contact

9. 12. From ice in the veins, through unfrozen fish and frozen frogs to isochoric preservation, ad Astra

10. 142 Nulliparity alters gene expression in inner cell mass and trophoblast of equine blastocysts in old mares

11. 17β-Estradiol alleviates intervertebral disc degeneration by inhibiting NF-κB signal pathway

12. 186 The effects of different concentrations of MgSO4 in osteogenic differentiation

13. 18β-Glycyrrhetinic acid inhibits IL-1β-induced inflammatory response in mouse chondrocytes and prevents osteoarthritic progression by activating Nrf2

14. 1H NMR spectroscopic studies establish that heparanase is a retaining glycosidase

15. 1α,25-Dihydroxyvitamin D3 and rosiglitazone synergistically enhance osteoblast-mediated mineralization

16. 1α,25-dihydroxyvitamin D3 Attenuates TGF-β-Induced Pro-Fibrotic Effects in Human Lung Epithelial Cells through Inhibition of Epithelial–Mesenchymal Transition

17. 1α,25-dihydroxyvitamin D3 attenuates oxidative stress-induced damage in human trabecular meshwork cells by inhibiting TGFβ-SMAD3-VDR pathway

18. 2,5-Dimethoxy 2,5-dihydrofuran crosslinked chitosan fibers enhance bone regeneration in rabbit femur defects

19. 2-Alkyl-anthraquinones inhibit Candida albicans biofilm via inhibiting the formation of matrix and hyphae

20. 20(S)- Protopanaxadiol suppresses hepatic stellate cell activation via WIF1 demethylation-mediated inactivation of the Wnt/β-catenin pathway

21. 20S-Protopanaxatriol Ameliorates Hepatic Fibrosis, Potentially Involving FXR-Mediated Inflammatory Signaling Cascades

22. 217 Induction and differentiation of porcine induced pluripotent stem cells into neuronal precursor cell-like cells

23. 22. Avoiding the problems of ice in tissues via vitrification

24. 244 chondrogenic pellet cultures for cartilage tissue engineering grow by deposition of matrix and not by cellular proliferation

25. 3,4-Methylenedioxy-β-nitrostyrene inhibits adhesion and migration of human triple-negative breast cancer cells by suppressing β1 integrin function and surface protein disulfide isomerase

26. 3-D mineralized silk fibroin/polycaprolactone composite scaffold modified with polyglutamate conjugated with BMP-2 peptide for bone tissue engineering

27. 3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture

28. 3-Dimensional porous nanocomposite scaffolds based on cellulose nanofibers for cartilage tissue engineering: tailoring of porosity and mechanical performance

29. 3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model

30. 30. Thermal properties of porcine myocardial tissue at subzero temperatures: Implication for thermal therapy studies

31. 3D Bioprinting Using Cross-Linker-Free Silk–Gelatin Bioink for Cartilage Tissue Engineering

32. 3D Bone tissue engineered with bioactive microspheres in simulated microgravity

33. 3D Cocultures of Osteoblasts and Staphylococcus aureus on Biomimetic Bone Scaffolds as a Tool to Investigate the Host–Pathogen Interface in Osteomyelitis

34. 3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

35. 3D Microcontact Printing for Combined Chemical and Topographical Patterning on Porous Cell Culture Membrane

36. 3D Porous poly(lactic acid)/regenerated cellulose composite scaffolds based on electrospun nanofibers for biomineralization

37. 3D Scaffolds with Different Stiffness but the Same Microstructure for Bone Tissue Engineering

38. 3D Tissue Engineered Supramolecular Hydrogels for Controlled Chondrogenesis of Human Mesenchymal Stem Cells

39. 3D Toolbox to Enhance Physiological Relevance of Human Tissue Models

40. 3D bio-printed biphasic scaffolds with dual modification of silk fibroin for the integrated repair of osteochondral defects

41. 3D bioprinting dermal-like structures using species-specific ulvan

42. 3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering

43. 3D cell printing of islet-laden pancreatic tissue-derived extracellular matrix bioink constructs for enhancing pancreatic functions

44. 3D cell-printing of biocompatible and functional meniscus constructs using meniscus‐derived bioink

45. 3D heterogeneous islet organoid generation from human embryonic stem cells using a novel engineered hydrogel platform

46. 3D multi-layered fibrous cellulose structure using an electrohydrodynamic process for tissue engineering

47. 3D organizational mapping of collagen fibers elucidates matrix remodeling in a hormone-sensitive 3D breast tissue model

48. 3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells

49. 3D porous scaffolds from wheat glutenin for cultured meat applications

50. 3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac repair

51. 3D scaffolds in breast cancer research

52. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes

53. 3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment

54. 3D-Bioprinted Difunctional Scaffold for In Situ Cartilage Regeneration Based on Aptamer-Directed Cell Recruitment and Growth Factor-Enhanced Cell Chondrogenesis

55. 3D-bioprinted BMSC-laden biomimetic multiphasic scaffolds for efficient repair of osteochondral defects in an osteoarthritic rat model

56. 3D-printed oxygen-releasing scaffolds improve bone regeneration in mice

57. 3DICE coding matrix multidirectional macro-architecture modulates cell organization, shape, and co-cultures endothelization network

58. 4-Methylumbelliferone Inhibits Angiogenesis in Vitro and in Vivo

59. 4. The promises of tissue engineering for organ building and banking

60. 5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

61. 52 Decellularization of goat uterus as a promising 3-dimensional homing matrix of biological scaffold: A pilot study

62. 5α-Dihydrotestosterone enhances wound healing in diabetic rats

63. 6-Month aortic valve implantation of an off-the-shelf tissue-engineered valve in sheep

64. 7. An approach to molecular characteristic of collagen mesh extracellular matrix in processed tissue banking, by diffractive techniques

65. HD-AGPs as Speciation Genes: Positive Selection on a Proline-Rich Domain in Non-Hybridizing Species of Petunia, Solanum, and Nicotiana

66. Perilla frutescens Sprout Extract Protect Renal Mesangial Cell Dysfunction against High Glucose by Modulating AMPK and NADPH Oxidase Signaling

67. A 3-D in vitro co-culture model of mammary gland involution

68. A 32-Kilodalton Hydrolase Plays an Important Role in Paracoccidioides brasiliensis Adherence to Host Cells and Influences Pathogenicity

69. A 3D bioinspired highly porous polymeric scaffolding system for in vitro simulation of pancreatic ductal adenocarcinoma

70. A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss