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Joana Soares; Teresa Neuparth; Angeliki Lyssimachou; Daniela Lima; Ana André; Maria A. Reis-Henriques; Luís Filipe C. Castro; António Paulo Carvalho; Nuno M. Monteiro; Miguel M. Santos
... Although chemicals are usually present in the environment in complex mixtures, ecotoxicological risk assessments often rely on data from single exposures to contaminants. The present study aimed at examining the effects of two ubiquitous endocrine disrupting chemicals (EDCs), 17α-ethynilestradiol (EE2) and tributyltin (TBT), single and combined, in zebrafish (Danio rerio) reproduction and embryoni ...
... Primordial germ cells (PGC) are the precursors of male and female progenitor cells. The cells are considered a valuable genetic material for the production of transgenic poultry. This technology includes isolation of the PGC from chick donor embryos, transformation of the cells, and injection into the dorsal aorta of recipient embryos. After injection, the PGC are involved in the process of embryo ...
... To assess the toxic effects of 3-(4-Methylbenzylidene) camphor (4-MBC) at environmentally relevant concentrations on the reproduction and development of Japanese medaka (Oryzias latipes), adult paired medaka (F0) were exposed to 5, 50, and 500 μg/L 4-MBC for 28 d in the current study. The fecundity and fertility were significantly decreased at 500 μg/L 4-MBC (p < 0.05). Histological observations s ...
DNA methylation; DNA replication; Y chromosome; adulthood; amino acids; antigens; apoptosis; blastocyst; chromatin; complement; demethylation; epigenetics; females; genes; gonads; histones; in vitro fertilization; males; metabolites; mice; mitochondria; oxygen; peptides; phenotype; polymerase chain reaction; sex determination; statistical analysis; uterus
Abstract:
... Previous research has shown that embryos conceived by IVF show a sexual dimorphic phenotype and different metabolite expression into adulthood. Interestingly, female embryos have a higher apoptotic rate and are more vulnerable to environmental stressors. Our prior studies have examined DNA methylation changes in male and female embryos; however, limited knowledge is available with regard to chroma ...
... Germ cell development and differentiation is a complex process associated with down-regulation of stem cell-associated genes and up-regulation of markers of germ cell differentiation and meiosis. In horses, putative primordial germ cells (PGCs) were identified outside the gonads starting 20 days after ovulation (Curran et al. 1997 Equine Vet. J. Suppl. 25, 72-76). However, no information is availa ...
... In anuran ovaries, 5S rDNA is regulated transcriptionally by transcription factor IIIA (TFIIIA), which upon transcription, binds 5S rRNA, forming 7S RNP. 5S rRNA can be stockpiled also in the form of 42S RNP bound to 42sp43. The aim of the present study was to assess the differential transcriptional regulation of 5S rRNA and associated proteins in thicklip gray mullet (Chelon labrosus) gonads. Up ...
... Oogenesis of the freshwater mussel Anodonte cygnea was investigated through histological examination over a 12-month period. All specimens were hermaphrodites, having gonads made up of numerous separate female and male acini. Female acini included oogonia with oocytes at different stages of development. Immature oocytes were attached to the acinar wall by a stalk, while mature oocytes were free wi ...
... A novel and simple method for isolating viable gonadal germ cells (GGCs) was developed in the domestic chicken, Gallus domesticus. Developing gonads were isolated from 7-day-old chick embryos and cultured for up to 24 hours at 37.8 degrees C in phosphate buffered saline without Ca2+ and Mg2+ (PBS[-]). A discharge of GGCs from the gonad was observed within 30 minutes after introducing the embryonic ...
... The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of Takifugu rubripes, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of T. rubripes (F, M, and PM) for high-throughput transcriptome sequencing. The results pr ...
Cantharidae; X chromosome; autosomes; centromeres; chromosome banding; chromosome number; females; gonads; karyotyping; larvae; males; meiosis; British Isles
Abstract:
... The karyotypes of 20 species of Cantharidae occurring in the British Isles are described and illustrated, 19 of which are hitherto unrecorded. These are Cantharis cryptica Ashe, Cantharis fusca Linnaeus, Cantharis lateralis Linnaeus, Cantharis livida Linnaeus, Cantharis nigra (Degeer), Cantharis nigricans (O. F. Müller), Cantharis pallida Goeze, Cantharis pellucida Fabricius, Cantharis rufa Linnae ...
Crassostrea gigas; Crassostrea virginica; diploidy; fecundity; females; gametogenesis; genetic improvement; gonads; males; mortality; oocytes; oysters; time series analysis; triploidy
Abstract:
... Triploidy is a common form of genetic improvement that confers benefits associated with reduced fecundity. Triploid oysters produce fewer gametes yet undergo gametogenesis and develop gonads to variable extents. The variable and typically abnormal gonad development in triploid oysters has often been summarized using criteria developed for the more uniform development of diploid oysters, which can ...
Cynoglossus semilaevis; females; gene expression regulation; genes; genetic markers; genome-wide association study; genotyping by sequencing; gonads; linkage disequilibrium; loci; males; messenger RNA; polymerase chain reaction; screening; sexual development; single nucleotide polymorphism
Abstract:
... Chinese tongue sole (Cynoglossus semilaevis) males and females exhibit great differences in growth rate and appearance. The species is heterogametic (ZW/ZZ) and has sex-reversed “pseudomales” that are genetically female and physiologically male. In this study, we identified eight sex-specific single nucleotide polymorphism (SNP) markers for the sex identification of C. semilaevis by using a combin ...
... The fat content and fatty acid profile of commercially important Seriola fasciata (Lesser amberjack) was determined in samples of muscle, liver and gonads of both genders at pre-spawning and spawning stage. Results indicated that edible muscle of pre-spawning fish had high fat content (>8%), lowering to medium fat (4–8%) content during spawning stage. Livers and gonads presented more constant and ...
... The physiological condition of two invasive crayfish species identified in Croatian rivers (Orconectes limosus and Pacifastacus leniusculus) was compared with that of native crayfish populations (Astacus astacus and A. leptodactylus). Hepatosomatic, gonadosomatic and abdominal muscle indices were examined for all four species and within a specific invadernative pair. The results demonstrate that i ...
acetates; adults; agonists; androgen receptors; bioactive properties; early development; environmental exposure; fecundity; feedlots; females; fish; gonads; in vitro studies; isomers; livestock; masculinization; metabolites; risk; sex hormones; sex ratio; signal transduction; steroid metabolism; surface water; trenbolone; uncertainty
Abstract:
... Trenbolone acetate is widely used in some parts of the world for its desirable anabolic effects on livestock. Several metabolites of the acetate, including 17β‐trenbolone, have been detected at low nanograms per liter concentrations in surface waters associated with animal feedlots. The 17β‐trenbolone isomer can affect androgen receptor signaling pathways in various vertebrate species at comparati ...
... We examined transgenerational effects of exposure to copper on early development of offspring in the urchin Evechinus chloroticus, and whether parental exposure mediated offspring response to a further low level exposure to copper during early development. Month-long exposure to elevated copper (25 μgL−1) in the laboratory increased copper in gonads of both males and females and reduced gonad size ...
... Induction of yolk protein in male fish is a recognized biomarker for estrogenic exposure because the estrogen-dependent induction mechanism is well investigated and there is a clear difference in yolk protein levels of unexposed males and females. Attempts have been made to use induction of bivalve yolk protein as biomarker for estrogenic exposure. However, several biomarker validation criteria ha ...
... Gametogenesis was monitored histologically in wild‐caught red snapper (Lutjanus campechanus, Poey) maintained in captivity under simulated natural photothermal conditions. Gonads were collected every 2–3 weeks (average n = 14) for histology during the pre‐spawning season (February to May, temperature increasing from 16°C to 24°C). Primary vitellogenic oocytes were first observed in one female when ...
... The Wolbachia are intracellular endosymbionts widely distributed among invertebrates. These primarily vertically transmitted α‐proteobacteria have been intensively studied during the last decades because of their intriguing interactions with hosts, ranging from reproductive manipulations to mutualism. To optimize their vertical transmission from mother to offspring, the Wolbachia have developed fi ...
... Hybridization is an important technique and is widely used in fish genetic breeding; this technique prevents variety degeneration and improves variety. In this research, we obtained a hybrid lineage (WR-F1-F4) by interspecific hybridization derived from female white crucian carp (Carassius cuvieri, WCC) and male red crucian carp (Carassius auratus red var., RCC), and a new type of improved fish (W ...
... The novel brominated flame retardants (NBFRs), bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (TBPH) and 2-ethylhexyl-2,3,4,5 tetrabromobenzoate (TBB) are components of the flame retardant mixture Firemaster 550 and both TBPH and TBB have recently been listed as high production volume chemicals by the US EPA. These NBFRs have been detected in several environmental matrices but very little is known ...
Tamanna Sultana; Sabuj Kanti Mazumder; Jannatul Kubra; Nurnobi Nishad; Sarker Mohammad Ibrahim Khalil; Simon Kumar Das; Md. Arifur Rahman Khan; Md. Tawheed Hasan
... The study aims to afford a deeper knowledge on reproductive biology of Mystus bleekeri from Surma River, Bangladesh in a multidisciplinary way. Though, the species is in demand as food and ornamental fish in Bangladesh but still now comprehensive details on reproductive biology of this species is scanty. Total 600 fish samples were derived from commercial catches from July 2018 to June 2019. Perio ...
... The fem-1 gene in Caenorhabditis elegans is involved in sex differentiation; it is specifically required for all aspects of male development. In this study, the full-length cDNA of the fem-1 (Pvfem-1) gene was isolated from the Pacific whiteleg shrimp Penaeus vannamei. The Pvfem-1 transcript is 3778 nt long and encodes a putative protein (PvFEM-1) of 638 amino acids that presented eight ankyrin re ...
brain; chromosome number; females; fetus; gender differences; gene expression; gonads; immune system; males; mammals; masculinization; sex chromosomes; signal transduction; transcriptomics
Abstract:
... Establishment of enduring sex differences in brain and behavior occurs during pre- or perinatal development, depending on species. For over 50 years the focus has been on gonadal steroid production by male fetuses and the impact on developing brain. An increasing awareness of the importance of sex chromosome complement has broadened the focus but identifying specific roles in development has yet t ...
Anguilla anguilla; Anguilla japonica; cold; eel; females; gonads; oocytes; water temperature
Abstract:
... To test whether gonadal development of female eels could be promoted without any exogenous hormone treatments, we observed the effect of water temperature manipulation. After 3–5 months of water temperature treatments, three silver eels showed higher gonadosomatic indices (GSI). In particular, one eel in the 5–15°C fluctuating temperature treatment group (5°C daytime and 15°C night‐time) had the h ...
... ORD protein is required for accurate chromosome segregation during male and female meiosis in Drosophila melanogaster. Null ord mutations result in random segregation of sister chromatids during both meiotic divisions because cohesion is completely abolished prior to kinetochore capture of microtubules during meiosis I. Previous analyses of mutant ord alleles have led us to propose that the C-term ...
... Sea mussels (genus Mytilus) have two mitochondrial genomes in obligatory co-existence, one that is transmitted through the egg and the other through the sperm. The phenomenon, known as Doubly Uniparental Inheritance (DUI) of mitochondrial DNA (mtDNA), is presently known to occur in more than 40 molluscan bivalve species. Females and the somatic tissues of males contain mainly the maternal (F) geno ...
Salmo salar; females; fish culture; food consumption; gonads; males; ovulation; oxygen consumption; rearing; salmon; seawater; solar radiation; spawning; summer; tanks; temperature
Abstract:
... This report is based on a finding of spent males and overripe females of Atlantic salmon on 30 August 2013. The studied fish population had been reared in tanks from seawater transfer in May 2011 to April 2013, and then in a sea-cage under natural light. From May 2011 until August 2012, and then again from February until April 2013, the fish were under natural light and temperature. In between the ...
Crustacea; Sciaenidae; Trachurus japonicus; Trichiurus; cannibalism; case studies; cluster analysis; coasts; diet; ecosystems; females; fish; fisheries; fishery resources; gonads; juveniles; maturity stage; predators; spawning; stomach; surveys; East China Sea
Abstract:
... The spatial distribution of fish on feeding grounds is an important factor in determining their prey composition. Of the factors that may influence the spatial distribution on the feeding ground and subsequent diet composition is the fish maturation stage. Using hairtail (Trichiurus japonicus) as an example, we evaluated the impacts of gonad development stage on diet composition. Hairtail supports ...
Rebecca Milsk; Jenna E. Cavallin; Elizabeth J. Durhan; Kathleen M. Jensen; Michael D. Kahl; Elizabeth A. Makynen; Dalma Martinović -Weigelt; Nathan Mueller; Anthony Schroeder; Daniel L. Villeneuve; Gerald T. Ankley
... The aim of this study was to investigate temporal changes in the hypothalamic-pituitary-gonadal (HPG) axis of fathead minnows (Pimephales promelas) treated with the model androgen receptor (AR) antagonist flutamide. Reproductively-mature fish were exposed in a flow-through test to analytically-confirmed concentrations of either 50 or 500μg flutamide/L for 8 d, followed by an 8-d recovery period in ...
Gerald T. Ankley; Jenna E. Cavallin; Elizabeth J. Durhan; Kathleen M. Jensen; Michael D. Kahl; Elizabeth A. Makynen; Linnea M. Thomas; Leah C. Wehmas; Daniel L. Villeneuve
... The objective of this study was to evaluate temporal effects of the model steroidogenesis inhibitor ketoconazole (KTC) on aspects of reproductive endocrine function controlled by the hypothalamic-pituitary-gonadal (HPG) axis in the fathead minnow (Pimephales promelas). Ketoconazole inhibits the activity of two cytochrome P450s (CYPs) key to sex steroid production in vertebrates, CYP11a (cholestero ...
... Apolipoprotein B mRNA-editing enzyme catalytic subunit 2 (APOBEC2) plays an important role in regulating and maintaining muscle development in mammals. In this study, we evaluated APOBEC2 mRNA abundance and protein expression and the results indicated that APOBEC2 mRNA was most abundant in skeletal and cardiac muscle, with relatively low expression in the gonads, gizzard and subcutaneous fat tissu ...
... Fecundity and mass of egg, as well as survival of offspring, of hatchery-reared small (less than 406 g) females of Atlantic salmon are determined. The absolute fecundity and mass of egg of artificially reared fish are sufficiently less than that of anadromous spawners. Small females are characterized by delayed spawning; histological analysis has demonstrated significant asynchrony in oocyte devel ...
... Based on the five wild Chinese sturgeons which were captured from the Yichang section of the Yangtze River, and the 323 offsprings of the Chinese sturgeons, were studied as to their survival, development and behaviour under aquarium conditions between 2005 and 2007 in the captive environment of an aquarium. All five wild caught females survived, with weight gains ranging from 0.55% (Specimen W1#: ...
... Total lipid content and lipid class composition were analyzed in gonads, liver and white muscle of yellowfin tuna (Thunnus albacares) throughout ovary development to understand its reproductive allocation strategy and to assess the relation between female condition and reproduction. A total of 112 females were collected onboard purse-seiner in the Western Indian Ocean from January to March 2009, f ...
... Ensuring supplies of pearl oyster spat for commercial grafting operations in Mexico is an ongoing problem. This has refocused research toward improving hatchery propagation protocols. Since gender plays an important role in the physiology of bivalves, we studied the use of fatty acids in the gonad and digestive gland of male and female winged pearl oyster (Pteria sterna) over its natural breeding ...
... Catnip (Nepeta cataria) is a popular plant among cat owners because in about 60% of felids elicits active behaviors such as rolling over, grooming, motor activity and vocalizations. Herein, we assessed the display of active but also passive responses, such as time in sphinx-like position, and consequently hypothesized that 100% of cats respond to catnip. Accordingly, sixty domestic cats of differe ...
... During Drosophila aging mortality rate increases exponentially and progeny production per animal declines dramatically, correlating with decreased number and division of somatic and germ-line stem cells in the gonads. To search for genes that might promote both longevity and fecundity, a P element transposon (PdL), containing an outwardly directed, doxycycline-inducible promoter was used to genera ...
... Many species of teleost fish live in coral reefs and change sex depending on their social status; some even demonstrate sex change in both directions. Typically, in the absence of a more dominant competitive individual, the fish functions as a male; however, when a more competitively superior individual arrives, the focal fish becomes a female. Among these bidirectional sex changers, there are spe ...
age determination; elderly; females; gonads; juveniles; males; otoliths; perch; reproduction; rivers; spawning; spring; summer; Argentina
Abstract:
... Age, growth and reproductive characteristics of creole perch, Percichthys trucha, were investigated in the Negro River, southern Argentina from samples collected seasonally, December 1994-December 1995. Age was estimated via scale and whole otolith reading methods. Total length (n = 413) ranged from 103 to 432 mm, and weight from 12 to 1042 g. Significant differences between the length-weight rela ...
Cyprinidae; age structure; females; fish; males; otoliths; rivers; sexual maturity; sport fishing; South Africa
Abstract:
... Age structure, growth and size at sexual maturity of smallmouth yellowfish, Labeobarbus aeneus (Burchell), were assessed in 2008–2009. Despite its angling and food importance, little has been published on many aspects of its ecology and biology, specifically its age structure in the Vaal River system. Of 193 L. aeneus collected by gillnetting, 96 were assessed for sexual maturity, and ages were es ...
... Bonefish (Albula spp.) are a prized sportfish among avid anglers worldwide. Two morphologically indistinguishable species of bonefish (Albula vulpes and Albula goreensis) occur in coastal areas of the tropical western Atlantic. Growth rates of A. vulpes, which supports an economically important sport fishery, differ among locations where they have been studied, but many locations have not yet been ...
Anguilla anguilla; Anguilla marmorata; females; gonadosomatic index; gonads; growth rings; life history; males; otoliths; rivers; water temperature; Japan
Abstract:
... Age, growth, and gonadal condition of the Giant mottled eel, Anguilla marmorata, were estimated from 303 specimens collected at Okinawa-jima Island, Japan. Otolith opaque zones that formed every year correlated with high water temperature and were thought to be annual rings. The growth of this species differed between males and females, and females were longer lived and larger than males. The mean ...
Platycephalus indicus; body size; females; fisheries; gonads; hermaphroditism; life history; males; otoliths; resource management; sex reversal; spawning; traditional technology; Japan
Abstract:
... Indian flathead Platycephalus indicus (Platycephalidae) is an important fishery resource in tropical and subtropical waters. However, there have been few studies of its life history which is important for resource management because of taxonomic confusion. Here, we determine the age, growth, maturity, and spawning season of P. indicus from 351 specimens [21.7–76.9 cm total length (TL) for females; ...
... This is the first study to investigate the age, growth and reproductive biology of the Atlantic spadefish Chaetodipterus faber in southern Brazil. A total of 625 individuals [total length (TL) ranging from 7 mm to 510 mm] were sampled at the Paranaguá Estuarine Complex (PEC) and adjacent coastal areas over a year (August 2015 to July 2016). Age estimation based on sagittal otolith cross section sh ...
... In the present study, life-history traits of importance to fisheries management were estimated for the forktail rabbitfish (Siganus argenteus) based on fishery-dependent samples collected over a 2-year period in the Commonwealth of the Northern Mariana Islands. Age-based and reproductive information was derived from analysis of sagittal otoliths and gonads. The species is short-lived, with a maxim ...
... The demography and sexual pattern of the white-streaked grouper, Epinephelus ongus (Serranidae), in the Yaeyama Islands, Okinawa, were examined based on age assessment using otoliths and gonadal histology. The von Bertalanffy growth parameters were estimated as follows: L∞ = 34.35 cm total length (TL); K = 0.187 yr. ⁻¹; and t₀ = − 0.975 yr. The maximum age was 20 yrs. The smallest mature female wa ...
... Seasonally breeding animals exhibit profound physiological and behavioural responses to changes in ambient day length (photoperiod), including changes in reproductive function and territorial aggression. Species where aggression persists when gonads are regressed and circulating levels of gonadal hormones are low, such as Siberian hamsters (Phodopus sungorus) and song sparrows (Melospiza melodia), ...
Anura; Caudata; Gymnophiona; acaricides; adults; agrochemicals; females; frogs; gonads; human health; juveniles; life history; males; metamorphosis; phonotaxis; reproductive success; sex reversal; thyroid hormones
Abstract:
... Concern over global amphibian declines and possible links to agrochemical use has led to research on the endocrine disrupting actions of agrochemicals, such as fertilizers, fungicides, insecticides, acaricides, herbicides, metals, and mixtures. Amphibians, like other species, have to partition resources for body maintenance, growth, and reproduction. Recent studies suggest that metabolic impairmen ...