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... Contamination and degradation of lipid moieties result in the formation of volatile compounds that affect the flavor and safety of food products. A wide variety of analytical techniques have been developed to determine the concentration of volatile flavor components in foods, such as vacuum distillation, headspace analysis, supercritical fluid extraction, and solid phase microextraction. Previousl ...
... The accumulation of volatile flavour constituents in liquid cultures of three Kluyveromyces lactis strains was studied after cultivation on defined culture media containing glucose as carbon source, yeast extract, vitamines, and different additional nitrogen compounds. Besides short-chain alcohols and esters (fruit esters), 2-phenylethyl alcohol, phenylacetaldehyde, and 2-phenylethyl esters could ...
Citrus; fruit juices; food composition; flavor compounds; bitterness
Abstract:
... Performance of the previously reported enzyme immunoassay for determination of limonin in citrus juice was improved by stabilization of anti-limonin-antibody-coated microtitration wells with 1% solution of ovalbumin and polyvinyl alcohol. Standard curves of the antibody stabilized wells showed insignificant change upon storage at 4 degrees C for 5 months. Average inter- and intra-assay coefficient ...
... Volatile oils isolated by steam distillation of fresh edible kelps (Laminaria angustata, L. japonica, Kjellmaniella crassifolia, Costaria costata, Ecklonia cava, Alaria crassifolia, Undaria pinnatifida) were analyzed by GC and GC-MS. The major component characterized in the volatile oils of the kelps was isolated and identified as a sesquiterpene alcohol known as cubenol. Flavor evaluation determi ...
... Samples of commercial orange juice packaged aseptically and stored at 21 degrees C and 26 degrees C were evaluated weekly by a sensory panel using hedonic ratings. Flavor scores were significantly lower than control juice and unacceptable after 1 wk at 26 degrees C and 2 wk at 21 degrees C. The flavor score decreased to 50-60% of the starting value during 6 wk storage. The volatile components, alp ...
... Volatile components of crayfish processing waste were analyzed by dynamic headspace/capillary gas chromatography/mass spectrometry and chromatography-coupled aroma perception analysis with simultaneous photoionization detection. One hundred seventeen compounds were identified. The majority of the flavor compounds previously identified in freshly boiled crayfish tail meat and hepatopancreas also we ...
peanuts; raw foods; roasting; volatile compounds; gas chromatography; flavor compounds
Abstract:
... A rapid headspace analysis method for evaluating the flavor quality of peanuts using gas chromatography has been developed and tested. Samples are ground, heated in glass vials for 30 min at 150 degrees C, and the headspace injected using a gas tight syringe. After initial startup, one can screen samples for flavor defects at the rate of four per hour. Of the peaks produced from peanut headspace a ...
... The effects of chemical modifications, esterification or reductive alkylation, on binding properties of several terpenes by beta-lactoglobulin were examined. As shown by fluorescence quenching, beta-lactoglobulin and its derivatives bind beta-ionone, but it does not bind alpha-ionone, neither geraniol nor R(+)- and S(-)-limonene. It is suggested that its binding site, presumably situated within th ...
passion fruits; flavor compounds; fruit juices; hydrolysis; terpenoids; volatile compounds
Abstract:
... Enzymatic hydrolysis of C18, reversed-phase isolates from the juice of the purple passion fruit led to the identification of a wide range of terpenoids. In addition to the main component linalool and other monoterpenoids, the following C13 norterpenoid aglycons were identified for the first time in passion fruit: 4-hydroxy-beta-ionol; 4-oxo-beta-ionol; 4-hydroxy-7,8-dihydro-beta-ionol; 4-oxo-7,8-d ...
... 1H NMR examination of 3-methoxy-2-(1'-methylpropyl)pyrazine 1-oxide (2) derived from 2-methoxy-3-(1'-methylpropyl)pyrazine (1), with use of achiral and chiral lanthanide shift reagents, showed that the enantiomeric composition of 1 isolated from Galbanum oil is 100% (+). Signal assignment of enantiomers was achieved by comparison of 1H NMR spectra of four kinds of 2 derived from natural 1 and synt ...
peaches; volatile compounds; flavor compounds; gas chromatography
Abstract:
... Dynamic headspace concentration was used to trap peach flavor volatiles from a promising cultivar under development. Separation and identification of components were by high resolution gas chromatography and mass spectrometry. One hundred ten compounds including alcohols, aldehydes, alkanes, esters, ketones, aromatic hydrocarbons, sulfur-containing compounds and some miscellaneous compounds were p ...
grapefruits; plant fats and oils; gibberellic acid; triethylamine; flavor compounds
Abstract:
... The nootkatone content in grapefruit peel oil extracted from flavedo and the peel oil content of fruit receiving preharvest treatment with 20 or 50 ppm gibberellic acid (GA) and/or 50, 125, or 250 ppm 2-(3,4-dichlorophenoxy)triethylamine (DCPTA) were determined. Treatment by GA reduced the rate of increase in nootkatone concentration observed in control fruit with maturation, and the effect was do ...
Apium graveolens; celery; volatile compounds; flavor compounds; nitrogen fertilizers; chemical constituents of plants
Abstract:
... The volatiles of two varieties of celery (Apium graveolens L. var. dulce and rapaceum) were isolated both by Likens-Nickerson extraction and by Soxhlet extraction. The extracts were analyzed and quantified by two-dimensional capillary gas chromatography. Celery grown with different levels of organic and/or anorganic fertilizers was investigated. The addition of high levels of mineral and/or organi ...
oranges; food composition; essential oils; odors; flavor compounds; sensory evaluation; United States; Brazil
Abstract:
... One commercial sample of each of aqueous orange essence and orange essence oil from Brazil was compared with three samples of each product produced in Florida. Brazilian and U.S. products were qualitatively identical, but minor quantitative differences were found. Sensory panels noted aroma differences between aqueous essences and essence oils produced in the United States and Brazil; similar arom ...
... The "popcorn"-like aroma was identified as 2-acetyl-1-pyrroline. Cooked Della white rice contained almost 300 ng 2-acetyl-1-pyrroline per g (dry weight) rice, while cooked Lemont white rice (nonaromatic cultivar) contained 4 ng per g (dry weight). Della white rice contained almost four times more 2-acetyl-1-pyrroline as the average (73 ppb) in two batches of Jasmine white rice imported from Thaila ...
... The inclusion complexes of beta-cyclodextrin with two pairs of chiral flavor compounds, limonene and carvone, were used to evaluate the chiral interaction. Thermal transition was examined by DSC and crystalline properties by X-ray diffractometry. The overall DSC thermograms and X-ray diffraction patterns were very similar between the chiral isomers. A difference of about 3 joules/g complex was obs ...
... Screening 27 commercial lipases showed that enzymes from Candida cylindracea, Pseudomonas fluorescens and Mucor miehei (immobilized) promoted synthesis of selected low molecular weight esters in nonaqueous systems. Maximum production after 24 hr incubation was obtained with substrate concentrations of 0.05 mol/L for isopentyl acetate, 0.2 mol/L for ethyl butyrate and 0.3 mol/L for isopentyl butyra ...
... The concentration of geosmin was determined in freshly dredged and 2-wk relayed rangia clam (Rangia cuneata) samples in different seasons of the year. Vacuum distillation/solvent extraction using an internal standard was followed by multiple-level standard addition gas chromatography/selective ion monitoring mass spectrometry. Most coefficients of variation were lower than 10%. On a dry-weight bas ...
... A rapid, semi-quantitative prediction model was developed for retention of volatile organic flavoring compounds during spray drying. Nonequilibrium vapor phase flux and effective (liquid phase) diffusion coefficients of lemon oil and/or butanol isomers (n-, sec-, tert-) correlated with spray dryer retention. Prediction of retention of these compounds was dependent on the specific sample matrix and ...
... Chiral gamma-lactones from the raw flavor extract of strawberries and of some commercially available fruit-containing foods and beverages were directly stereoanalyzed by multidimensional gas chromatography (MDGC), employing heart-cutting techniques from DB 1701 as the preseparation column onto heptakis(3-O-acetyl-2,6-di-O-pentyl)-beta-cyclodextrin as the chiral stationary phase. ...
... Three populations of tobacco-doubled haploids (DH) were evaluated to characterize the relationship between density of secreting glandular trichomes (SGT) and levels (micrograms per square centimeter of leaf surface) of three trichome exudate constituents and to identify DH lines with altered concentrations of cis-abienol, sucrose esters containing beta-methylvaleric acid (BMVSE), and alpha- and be ...
Prunus armeniaca; apricots; flavor compounds; food analysis; gas chromatography; mass spectrometry; volatile compounds; extracts; California
Abstract:
... Volatile constituents of fresh apricots (Prunus armeniaca) of the Blenheim variety were analyzed by capillary gas chromatography and gas chromatography-mass spectrometry. The fruit was sampled by simultaneous vacuum steam distillation-extraction. A total of 49 components were identified in the extract, including 25 constituents reported for the first time in apricot. Linalool, lactones, and C6 lip ...
chemical composition; off flavors; isoprenoids; chemical analysis; wines; flavor compounds; degradation; glycosides
Abstract:
... In a glycosidic fraction of Riesling wine, obtained by retention of the glycosides on C18 reversed-phase adsorbent followed by enzymatic hydrolysis and trimethylsilylation of the liberated aglycons, capillary gas chromatography (HRGC) and capillary gas chromatography-mass spectrometry (HRGC-MS) revealed the occurrence of 2,6,10,10-tetramethyl-1-oxaspiro[4.5]dec-6-ene-2,8-diol. The identification w ...
apple juice; odors; flavor compounds; chemical analysis; solutions
Abstract:
... Infinite dilution activity coefficients of several alcohols, aldehydes, and esters, considered to be the major components of apple aroma, were measured in model food solutions containing glucose, fructose, sucrose, and malic acid. Measurements were made by following the dilution exponential method, at temperatures ranging from 25 to 68 degrees C. Experimental data were correlated with the one-para ...
... Pseudomonas fragi produced a pleasant strawberry-like odor when grown in skim milk at 15 degrees C. Volatiles from the culture broth were extracted and enriched by using a purge and trap method and analyzed by using sniffing FID-GC. FID revealed the presence of approximately 90 compounds. Concomitant olfactory analysis of the eluted compounds demonstrated that 26 were odor-active. Ethyl butyrate, ...
... A semiconductor gas sensor array was applied to discriminating coffee aromas, essential oils, and volatile compounds with different functional groups. To standardize sample introduction and to remove excess ethanol from volatile mixtures, headspace concentration utilizing a porous polymer trap was incorporated into the sensing system. Distinctive differences were not observed among response patter ...
... HRGC and HRGC-MS identifications of bound aroma compounds from the fruit and the leaves of blackberry (Rubus laciniata L.) were achieved after isolation of extracts obtained by Amberlite XAD-2 adsorption and methanol elution followed by hydrolysis with a commercial pectinase enzyme. In the fruit 41 aglycons and in the leaves 32 bound aroma compounds were identified. Quantitative HRGC revealed 5.8 ...
flavor compounds; isomers; molecular conformation; nuclear magnetic resonance spectroscopy
Abstract:
... A series of NOE experiments was carried out to clarify the conformation of cis- (1) and trans-3-hydroxy-alpha-damascone (2). For 1, half-chair conformation with a quasiequatorial hydroxy group and a quasiaxial side chain was determined. A main half-chair conformation with quasiequatorial positions both for the hydroxy group and for the side chain was also found for 2. According to the NOE experime ...
tryptophan; degradation; flavor compounds; food chemistry; kinetics; separation; solvents; temperature
Abstract:
... Tryptophan Amadori rearrangement product (ARP) was synthesized, and its decomposition was studied at two different temperatures and concentrations over a period of 6 h. RP-HPLC analysis of the reaction mixtures indicated the presence of hydroxymethylfurfural (HMF), maltol, tryptophan, indole, norharman and harman. Kinetic analysis showed that the activation energy (Ea) for the decomposition of ARP ...
... A direct competitive ELISA for geosmin (a musty/earthy off-flavor compound) was developed. Antibodies were produced in goats injected with a BSA-argosmin C (a dehydration product of geosmin) conjugate. The sensitivity and specificity of the antibody were respectively determined in a microtiter plate coated with the purified antibody which was subsequently detected with an alkaline phosphatase-argo ...
... Browning intensities of an L-cysteine/D-glucose model system with different moisture contents (0-40%) upon microwave irradiation for 2.5 min at the high setting of a 700-W microwave oven were measured by a UV spectrophotometer at 420 nm. The volatile compounds generated were analyzed by gas chromatography and mass spectrometry. Maximum browning intensity occurred at approximately 14% moisture. The ...
... Oils obtained from various parts of the basil plant were categorized into five groups by preference ranking test of their aromas. Among them the aroma of fresh leaf-flower oil was the most preferable, while that of stem was the least. According to the odor assessment of each volatile compound by the sniffing method of gas chromatography, methylehavicol and 1-octen-3-ol were the most and the least ...
dried foods; flavor compounds; volatile compounds; gas chromatography; temperature; squid
Abstract:
... The volatile components were produced by heating dried squid with glass beads and water. Two flavor concentrates (A and B), obtained by distillation at two temperature ranges, 125-190 and 215-230 degrees C, were analyzed by GC-MS. In addition to several headspace components, a total of 97 components were identified. Concentrate A gave a moderately roasted odor of dried squid and was characterized ...
tropical and subtropical fruits; chemical analysis; fruit pulp; flavor compounds; Solanum; odors; glycosides; linalool; Colombia
Abstract:
... HRGC and HRGC-MS identifications of bound aroma compounds from the pulp and the peelings of lulu fruit (Solanum vestissimum D.) were achieved after isolation of extracts obtained by Amberlite XAD-2 adsorption and methanol elution followed by hydrolysis with commercial pectinase enzymes. Whereas the aglycons identified in the peelings mainly consisted of terpenoids, with enantiomerically pure (R)-( ...
... HRGC and HRGC-MS identifications of bound aroma compounds from fruits of Prunus species (apricot, P. armeniaca, L.; peach, P. persica, L.; yellow plum, P. domestica, L. ssp. syriaca) were achieved after isolation of extracts obtained by Amberlite XAD-2 adsorption and methanol elution followed by simultaneous enzyme catalysis extraction (SECE) using beta-glucosidase (emulsin). In total, 47 bound ag ...
chemical composition; volatile compounds; chemical analysis; flavor compounds; pineapples; odors
Abstract:
... The volatiles of pineapple essence were isolated by liquid-liquid extraction, fractionated on neutral alumina, and analyzed by high-resolution gas chromatography and coupled gas chromatography/mass spectrometry (GC/MS). Twenty-six constituents (1 tentative) were identified for the first time in pineapple including the following sulfur compounds: methyl 3-(methylthio)-(E)-2-propenoate, methyl 3-(me ...
... Capillary gas chromatographic analysis of aroma extracts obtained by simultaneous distillation-extraction from yellow passion fruits (Passiflora edulis f. flavicarpa) using a flame photometric detector revealed the presence of previously unreported sulfur-containing volatiles. 3-Mercaptohexanol and the acetates, butanoates, and hexanoates of 3-mercaptohexanol and 3-(methylthio)hexanol, respectivel ...
... A stable isotope dilution assay has been developed for the quantitative determination of beta-damascenone in foods. Application of the method to roasted coffee (powder and brew), black tea, honey, and beer has indicated that the odorant can be determined with a high degree of sensitivity and accuracy. ...
black tea; caffeine; chemical composition; environmental factors; fertilizer application; flavor; flavor compounds; hens; nitrogen fertilizers; theaflavins; Kenya
Abstract:
... Though tea production continues throughout the year in Kenya, there ere monthly variations in yields and chemical composition attributable to the changes in environmental conditions. Total volatile flavour compounds (VFC), group I VFC, group II VFC and caffeine ere higher throughout the experimental period in black teas manufactured from plants here fertilisers ere applied than here fertiliser as ...
aldehydes; apple juice; chemical structure; esters; flavor; flavor compounds; juice concentrates; processing equipment; reverse osmosis; temperature; volatile compounds
Abstract:
... Plate‐and‐frame (PF) and spiral‐wound (SW) membrane configurations were used to study influence of reverse osmosis (RO) on flavor compounds in apple juice permeate and retentate. Low temperature (20°C) and high pressure (50 bar) were most effective operating parameters for flavor recovery. Non‐volatile flavor component RO losses were due to permeation. However, loss of volatile compounds through p ...
... Equimolar aqueous solutions of glycine, diglycine, triglycine, and tetraglycine were heated separately with D-glucose at 180 degree C at pH 4-5 in a Parr bomb for 2 h. The volatile compounds generated from the reaction were analyzed by gas chromatography and gas chromatography-mass spectrometry. The Maillard reaction of glucose with glycine and triglycine produced significantly greater amounts of ...
... The aroma concentrates from uncured and cured beef and chicken were isolated by the continuous steam distillation-extraction (SDE) method. By use of hexanal and decanal in pentane as the respective internal standards for cured and uncured meat, quantitative estimation of the volatile constituents in beef and chicken was carried out by using GC-MS. The investigation indicated that hexanal was found ...
raspberries; fruit pulp; odors; volatile compounds; flavor compounds; gas chromatography; glycosides; hydrolysis
Abstract:
... HRGC and HRGC-MS identifications of bound volatiles from raspberry fruit (Rubus idaeus cv. Heritage) pulp were achieved after isolation of an extract obtained by Amberlite XAD-2 adsorption and methanol elution followed by hydrolysis with a commercial pectinase enzyme. In total, 57 bound aglycons were identified for the first time in raspberry fruit originating from fatty acid, phenylpropanoid, and ...
packaging materials; food packaging; odors; flavor compounds; extraction; polyethylene film
Abstract:
... Supercritical carbon dioxide was used for extracting aroma compounds absorbed by low-density polyethylene. The extraction step was directly coupled with gas chromatography. The method yielded recoveries and reproducibilities similar to those obtained by a conventional liquid solvent extraction. The standard deviation ranged from 3 to 10% for the different species. The main advantages with the new ...
... The thermodynamics of binding was determined for soybean glycinin and beta-conglycinin and flavor ligands butanal, pentanal, hexanal, octanal, 2- and 3-hexanone, 2- and 5-nonanone, hexanol, and hexane. Hexane had affinity for these proteins only at 5 degrees C. Affinities of binding for all flavor ligands were greater for glycinin than beta-conglycinin. Affinity for aldehydes increased with increa ...