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1. Phylloseptin-PBa1, -PBa2, -PBa3: Three novel antimicrobial peptides from the skin secretion of Burmeister's leaf frog (Phyllomedusa burmeisteri)

Author:
Zhou, Mei,   et al.   ; Wu, Youjia; Wang, Lei; Chen, Tianbao; Shaw, Chris;     Show all 5 Authors
Source:
Biochemical and biophysical research communications 2019 v.509 no.3 pp. 664-673
ISSN:
0006-291X
Subject:
Cruziohyla; Gram-positive bacteria; Phyllomedusa; antimicrobial peptides; antimicrobial properties; antineoplastic activity; bioactive compounds; cDNA libraries; complementary DNA; frogs; hemolysis; secretion; tandem mass spectrometry; yeasts
Abstract:
... Skin secretions are known as a highly-complex mixture of abundant and diverse bioactive molecules and its study has attracted increasing attention over recent years. Phylloseptin is a unique family of antimicrobial peptides which have been only isolated from frogs of the Phyllomedusinae subfamily. Here, three novel peptide precursors were successfully cloned from a cDNA library, which was construc ...
DOI:
10.1016/j.bbrc.2018.12.156

2. Skin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning

Author:
Zhou, Mei,   et al.   ; Proaño-Bolaños, Carolina; Chen, Tianbao; Shaw, Chris;     Show all 4 Authors
Source:
Analytical biochemistry 2019 v.564-565 pp. 13-15
ISSN:
0003-2697
Subject:
Pelophylax; antimicrobial peptides; chromatography; frogs; messenger RNA; molecular cloning; secretion; skin (animal); transcriptome; transcriptomics
Abstract:
... Traditional sources of mRNA for molecular cloning on amphibian skin secretion studies have been the frog's skin and skin secretions. Here, we demonstrate that mRNA isolated from chromatographic fractions of skin secretions is amenable for molecular cloning assays. We identified precursor sequences of the Arg0, Trp5, Leu8-bradykinin and six antimicrobial peptides of Pelophylax esculentus (Ranidae). ...
DOI:
10.1016/j.ab.2018.10.006

3. A novel membrane-disruptive antimicrobial peptide from frog skin secretion against cystic fibrosis isolates and evaluation of anti-MRSA effect using Galleria mellonella model

Author:
Zhou, Mei,   et al.   ; Yuan, Yipeng; Zai, Yu; Xi, Xinping; Ma, Chengbang; Wang, Lei; Shaw, Chris; Chen, Tianbao;     Show all 8 Authors
Source:
Biochimica et biophysica acta 2019 v.1863 no.5 pp. 849-856
ISSN:
0304-4165
Subject:
Galleria mellonella; Gram-positive bacteria; antibiotic resistance; antimicrobial peptides; antimicrobial properties; biofilm; cell membranes; complementary DNA; cystic fibrosis; cytotoxicity; disease course; drugs; frogs; hemolysis; in vivo studies; insect larvae; methicillin; methicillin-resistant Staphylococcus aureus; models; mortality; patients; permeability; plankton; secretion; tandem mass spectrometry; China
Abstract:
... Antimicrobial peptides from amphibian skin secretion are a promising source for the development of alternative antibiotics against the urgent antibiotic resistance. Methicillin-resistant S. aureus (MRSA) has been found to persist in both early and late disease course of cystic fibrosis (CF). Japonicin-2LF was isolated from the skin secretion of Fujian Large-headed Frog (Limnonectes fujianensis) vi ...
DOI:
10.1016/j.bbagen.2019.02.013

4. Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea

Author:
Zhou, Mei,   et al.   ; Li, Lei; Wu, Qing; Wang, Xi; Lu, Huimin; Xi, Xinping; Watson, Chris J.; Chen, Tianbao; Wang, Lei;     Show all 9 Authors
Source:
International Journal of Genomics 2018 v.2018
ISSN:
2314-4378
Subject:
Candida albicans; Enterococcus faecalis; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Litoria caerulea; antibiotic resistance; bioactive compounds; bioactive properties; bladder; drugs; frogs; ileum; methicillin; methicillin-resistant Staphylococcus aureus; microbial growth; peptides; plankton; rats; secretion; smooth muscle; therapeutics; transcriptomics; yeasts
Abstract:
... Abundant biologically active peptides have been discovered from frog skin secretions, a rich natural source of bioactive compounds with great potential in drug discovery. In this study, three Caeridin peptides, namely, Caeridin-1, S5-Caeridin-1, and Caeridin-a1, were discovered from the skin secretion of the Australian White’s tree frog, Litoria caerulea, for the first time, by means of combining ...
DOI:
10.1155/2018/8158453

5. Peptidomic approach identifies cruzioseptins, a new family of potent antimicrobial peptides in the splendid leaf frog, Cruziohyla calcarifer

Author:
Zhou, Mei,   et al.   ; Proaño-Bolaños, Carolina; Wang, Lei; Coloma, Luis A.; Chen, Tianbao; Shaw, Chris;     Show all 6 Authors
Source:
Journal of proteomics 2016 v.146 pp. 1-13
ISSN:
1874-3919
Subject:
Candida albicans; Escherichia coli; Staphylococcus aureus; anti-infective properties; antibiotics; antifungal agents; antimicrobial peptides; bacteria; frogs; hemolysis; leaves; matrix-assisted laser desorption-ionization mass spectrometry; molecular cloning; new family; secretion; tandem mass spectrometry; yeasts
Abstract:
... Phyllomedusine frogs are an extraordinary source of biologically active peptides. At least 8 families of antimicrobial peptides have been reported in this frog clade, the dermaseptins being the most diverse. By a peptidomic approach, integrating molecular cloning, Edman degradation sequencing and tandem mass spectrometry, a new family of antimicrobial peptides has been identified in Cruziohyla cal ...
DOI:
10.1016/j.jprot.2016.06.017

6. Identification and bioactivity evaluation of two novel temporins from the skin secretion of the European edible frog, Pelophylax kl. esculentus

Author:
Zhou, Mei,   et al.   ; Chen, Xiaole; Wang, He; Yang, Mu; Wang, Lei; Chen, Tianbao; Shaw, Chris;     Show all 7 Authors
Source:
Biochemical and biophysical research communications 2016 v.476 pp. 566-573
ISSN:
0006-291X
Subject:
Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Pelophylax; Staphylococcus aureus; anti-infective agents; antimicrobial peptides; bioactive properties; cDNA libraries; clones; complementary DNA; drugs; frogs; hemolysis; hydrophobicity; molecular weight; reversed-phase high performance liquid chromatography; secretion; skin (animal)
Abstract:
... The amphibian temporins, amongst the smallest antimicrobial peptides (AMPs), are α-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLPVIAGVLSKLFamide) and temporin-1Re (FLPGLLAGLLamide), whose biosynthetic pr ...
DOI:
10.1016/j.bbrc.2016.05.163

7. Kunitzins: Prototypes of a new class of protease inhibitor from the skin secretions of European and Asian frogs

Author:
Zhou, Mei,   et al.   ; Chen, Xiaole; Wang, He; Shen, Yue; Wang, Lei; Chen, Tianbao; Shaw, Chris;     Show all 7 Authors
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
Candida albicans; Escherichia coli; Rana esculenta; Staphylococcus aureus; antibacterial properties; antimicrobial peptides; chymotrypsin; complementary DNA; disulfide bonds; freeze drying; frogs; hemolysis; prototypes; skin (animal); trypsin inhibitors
Abstract:
... Amphibian skin secretions contain biologically-active compounds, such as anti-microbial peptides and trypsin inhibitors, which are used by biomedical researchers as a source of potential novel drug leads or pharmacological agents. Here, we report the application of a recently developed technique within our laboratory to “shotgun” clone the cDNAs encoding two novel but structurally-related peptides ...
DOI:
10.1016/j.bbrc.2016.06.062

8. Hylaranins: prototypes of a new class of amphibian antimicrobial peptide from the skin secretion of the oriental broad-folded frog, Hylarana latouchii

Author:
Zhou, Mei,   et al.   ; Lin, Yan; Hu, Nan; Lyu, Peng; Ma, Jie; Wang, Lei; Guo, Suhua; Chen, Tianbao; Shaw, Chris;     Show all 9 Authors
Source:
Amino acids 2014 v.46 no.4 pp. 901-909
ISSN:
0939-4451
Subject:
Candida albicans; Escherichia coli; Staphylococcus aureus; amino acids; anti-infective properties; antimicrobial peptides; bacterial infections; cDNA libraries; complementary DNA; frogs; hemolysis; prototypes; reversed-phase high performance liquid chromatography; secretion; therapeutics; yeasts
Abstract:
... Amphibian skin secretions contain a broad spectrum of biologically active compounds, particularly antimicrobial peptides, which are considered to constitute a first line of defence against bacterial infection. Here we describe the identification of two prototype peptides representing a novel structural class of antimicrobial peptide from the skin secretion of the oriental broad-folded frog, Hylara ...
DOI:
10.1007/s00726-013-1655-1

9. Antimicrobial peptides and alytesin are co-secreted from the venom of the Midwife toad, Alytes maurus (Alytidae, Anura): Implications for the evolution of frog skin defensive secretions

Author:
Zhou, Mei,   et al.   ; König, Enrico; Wang, Lei; Chen, Tianbao; Bininda-Emonds, Olaf R.P.; Shaw, Chris;     Show all 6 Authors
Source:
Toxicon 2012 v.60 no.6 pp. 967-981
ISSN:
0041-0101
Subject:
Anura; amino acid sequences; anti-infective properties; antimicrobial peptides; complementary DNA; convergent evolution; defensive secretions; frogs; fuels; genes; innate immunity; minimum inhibitory concentration; neuropeptides; secretion; sequence analysis; tandem repeat sequences; toads; transcriptome; venoms
Abstract:
... The skin secretions of frogs and toads (Anura) have long been a known source of a vast abundance of bioactive substances. In the past decade, transcriptome data of the granular glands of anuran skin has given new impetus to investigations of the putative constituent peptides. Alytes obstetricans was recently investigated and novel peptides with antimicrobial activity were isolated and functionally ...
DOI:
10.1016/j.toxicon.2012.06.015

10. Molecular cloning of the trypsin inhibitor from the skin secretion of the Madagascan Tomato Frog, Dyscophus guineti (Microhylidae), and insights into its potential defensive role

Author:
Zhou, Mei,   et al.   ; König, Enrico; Wesse, Christina; C. Murphy, Anna; Wang, Lei; Chen, Tianbao; Shaw, Chris; Bininda-Emonds, Olaf R. P.;     Show all 8 Authors
Source:
Organisms, diversity, & evolution 2013 v.13 no.3 pp. 453-461
ISSN:
1439-6092
Subject:
adhesion; antimicrobial properties; cDNA libraries; cattle; complementary DNA; frogs; inflation; ingestion; molecular cloning; polymerization; secretion; thrombin; tomatoes; toxicity; trypsin; trypsin inhibitors
Abstract:
... In this study, we investigate the skin secretion of the Madagascan Tomato Frog, Dyscophus guineti, which is characterized by its peculiarly adhesive and viscous nature, with a view toward the function of the member of the Kunitz/bovine pancreatic trypsin inhibitor family (BPTI) it is known to contain. Using “shotgun” cloning of a skin secretion-derived cDNA library, we obtained the full-length seq ...
DOI:
10.1007/s13127-013-0128-4

11. Medusins: A new class of antimicrobial peptides from the skin secretions of phyllomedusine frogs

Author:
Zhou, Mei,   et al.   ; Xi, Xinping; Li, Renjie; Jiang, Yingchun; Lin, Yan; Wu, Yuxin; Xu, Jie; Wang, Lei; Chen, Tianbao; Shaw, Chris;     Show all 10 Authors
Source:
Biochimie 2013 v.95 pp. 1288-1296
ISSN:
0300-9084
Subject:
Agalychnis callidryas; Candida albicans; Gram-positive bacteria; Staphylococcus aureus; antibiotics; antimicrobial peptides; bacterial infections; complementary DNA; drugs; frogs; leaves; molecular cloning; multiple drug resistance; yeasts
Abstract:
... Natural drug discovery represents an area of research with vast potential. The investigation into the use of naturally-occurring peptides as potential therapeutic agents provides a new “chemical space” for the procurement of drug leads. Intensive and systematic studies on the broad-spectrum antimicrobial peptides found in amphibian skin secretions are of particular interest in the quest for new an ...
DOI:
10.1016/j.biochi.2013.02.005

12. The chains of the heterodimeric amphibian skin antimicrobial peptide, distinctin, are encoded by separate messenger RNAs

Author:
Zhou, Mei,   et al.   ; Evaristo, Geisa; Pinkse, Martijn; Wang, Lei; Wu, Youjia; Wang, Hui; Chen, Tianbao; Shaw, Chris; Verhaert, Peter;     Show all 9 Authors
Source:
Journal of proteomics 2013 v.78 pp. 245-253
ISSN:
1874-3919
Subject:
antimicrobial peptides; cDNA libraries; complementary DNA; cystine; dimerization; frogs; messenger RNA; nucleotide sequences; venoms
Abstract:
... Using a primer to a conserved nucleotide sequence of previously cloned skin peptides of Phyllomedusa species, two distinct cDNAs were “shotgun” cloned from a skin secretion-derived cDNA library of the frog, Phyllomedusa burmeisteri. The two ORFs separately encode chains A and B of an analog of the previously reported heterodimeric peptide, distinctin. LC-MS/MS analysis of native versus dithiotreit ...
DOI:
10.1016/j.jprot.2012.09.016
PubMed:
23017495

13. Bradykinin-related peptides (BRPs) from skin secretions of three genera of phyllomedusine leaf frogs and their comparative pharmacological effects on mammalian smooth muscles

Author:
Zhou, Mei,   et al.   ; Jiang, Yingchun; Xi, Xinping; Ge, Lilin; Yang, Nan; Hou, Xiaojuan; Ma, Jie; Ma, Chengbang; Wu, Yuxin; Guo, Xiaoxiao; Li, Renjie; Wang, Lei; Chen, Tianbao; Shaw, Chris;     Show all 14 Authors
Source:
Peptides 2014 v.52 pp. 122-133
ISSN:
0196-9781
Subject:
Agalychnis callidryas; Phyllomedusa; Ranidae; amino acid substitution; bioactive properties; biosynthesis; bradykinin; complementary DNA; frogs; hydroxylation; leaves; mammals; molecular cloning; post-translational modification; proline; receptors; smooth muscle; tyrosine
Abstract:
... While bradykinin has been identified in the skin secretions from several species of amphibian, bradykinin-related peptides (BRPs) are more common constituents. These peptides display a plethora of primary structural variations from the type peptide which include single or multiple amino acid substitutions, N- and/or C-terminal extensions and post-translational modifications such as proline hydroxy ...
DOI:
10.1016/j.peptides.2013.12.013

14. Limnonectins: A new class of antimicrobial peptides from the skin secretion of the Fujian large-headed frog (Limnonectes fujianensis)

Author:
Zhou, Mei,   et al.   ; Wu, Youjia; Wang, Lei; Ma, Chengbang; Chen, Xiaole; Bai, Bing; Chen, Tianbao; Shaw, Chris;     Show all 8 Authors
Source:
Biochimie 2011 v.93 no.6 pp. 981-987
ISSN:
0300-9084
Subject:
Gram-negative bacteria; antimicrobial peptides; cDNA libraries; complementary DNA; frogs; hemolysis; molecular cloning; nucleotide sequences; prototypes; secretion; signal peptide
Abstract:
... Amphibian skin secretions are rich sources of biologically-active peptides with antimicrobial peptides predominating in many species. Several studies involving molecular cloning of biosynthetic precursor-encoding cDNAs from skin or skin secretions have revealed that these exhibit highly-conserved domain architectures with an unusually high degree of conserved nucleotide and resultant amino acid se ...
DOI:
10.1016/j.biochi.2011.03.003

15. A fish bradykinin (Arg⁰, Trp⁵, Leu⁸-bradykinin) from the defensive skin secretion of the European edible frog, Pelophylax kl. esculentus: Structural characterization; molecular cloning of skin kininogen cDNA and pharmacological effects on mammalian smooth muscle

Author:
Zhou, Mei,   et al.   ; Chen, Xiaole; Wang, Lei; Wang, He; Chen, Hang; Chen, Tianbao; Shaw, Chris;     Show all 7 Authors
Source:
Peptides 2011 v.32 no.1 pp. 26-30
ISSN:
0196-9781
Subject:
Amia calva; Anarhichadidae; Danio rerio; Esox lucius; Gadus morhua; Lepisosteus; Oncorhynchus mykiss; Pelophylax; Tetraodon nigroviridis; amino acids; blood pressure; bradykinin; cod (fish); complementary DNA; cystatins; frogs; molecular cloning; open reading frames; predators; rats; secretion; smooth muscle; trout
Abstract:
... Extensive studies on bradykinin-related peptides (BRPs) generated from plasma kininogens in representative species of various vertebrate taxa, have confirmed that many amphibian skin BRPs reflect those present in putative vertebrate predators. For example, the (Val¹, Thr⁶)-bradykinin, present in the defensive skin secretions of many ranids and phyllomedusines, can be generated from plasma kininoge ...
DOI:
10.1016/j.peptides.2010.09.023

16. A novel Kazal-type trypsin inhibitor from the skin secretion of the Central American red-eyed leaf frog, Agalychnis callidryas

Author:
Zhou, Mei,   et al.   ; Li, Renjie; Wang, Hui; Jiang, Yingchun; Yu, Yang; Wang, Lei; Zhang, Yingqi; Chen, Tianbao; Shaw, Chris;     Show all 9 Authors
Source:
Biochimie 2012 v.94 no.6 pp. 1376-1381
ISSN:
0300-9084
Subject:
Agalychnis callidryas; Kunitz-type proteinase inhibitor; Osteichthyes; amino acids; complementary DNA; frogs; leaves; matrix-assisted laser desorption-ionization mass spectrometry; molecular weight; proteins; reversed-phase high performance liquid chromatography; secretion; trypsin
Abstract:
... The chemical complexity of the defensive skin secretion of the red-eyed leaf frog, (Agalychnis callidryas), has not been elucidated in detail. During a systematic study of the skin secretion peptidomes of phyllomedusine frogs, we discovered a novel Kazal-type protein with potent trypsin inhibitory activity (Ki = 1.9 nM) that displays the highest degree of structural similarity with Kazal proteins ...
DOI:
10.1016/j.biochi.2012.03.009

17. Functional peptidomics of amphibian skin secretion: A novel Kunitz-type chymotrypsin inhibitor from the African hyperoliid frog, Kassina senegalensis

Author:
Zhou, Mei,   et al.   ; Wang, Hui; Wang, Lei; Yang, Mu; Ma, Chengbang; Chen, Tianbao; Zhang, Yingqi; Zeller, Martin; Hornshaw, Martin; Shaw, Chris;     Show all 10 Authors
Source:
Biochimie 2012 v.94 no.3 pp. 891-899
ISSN:
0300-9084
Subject:
active sites; amino acids; bioinformatics; chymotrypsin; complementary DNA; fractionation; frogs; molecular cloning; molecular weight; polypeptides; screening; secretion; sequence analysis; silkworms; snakes; trypsin inhibitors
Abstract:
... Amphibian skin secretions are, for the most part, complex peptidomes. While many peptide components have been biologically- and structurally-characterised into discrete “families”, some of which are analogues of endogenous vertebrate regulatory peptides, a substantial number are of unique structure and unknown function. Among the components of these secretory peptidomes is an array of protease inh ...
DOI:
10.1016/j.biochi.2011.12.008

18. Identification and molecular cloning of a novel amphibian Bowman Birk-type trypsin inhibitor from the skin of the Hejiang Odorous Frog; Odorrana hejiangensis

Author:
Zhou, Mei,   et al.   ; Wang, Min; Wang, Lei; Chen, Tianbao; Walker, Brian; Sui, Dayuan; Conlon, J. Michael; Shaw, Chris;     Show all 8 Authors
Source:
Peptides 2012 v.33 no.2 pp. 245-250
ISSN:
0196-9781
Subject:
amino acid sequences; anti-infective properties; bioinformatics; cDNA libraries; complementary DNA; frogs; matrix-assisted laser desorption-ionization mass spectrometry; molecular cloning; molecular weight; nucleotide sequences; reversed-phase high performance liquid chromatography; trypsin; trypsin inhibitors
Abstract:
... In this study, an amphibian (Odorrana hejiangensis) skin extract was fractionated by reverse phase HPLC and fractions were screened for trypsin inhibitory activity. Using this initial approach, a novel trypsin inhibitory peptide was detected with an apparent protonated molecular mass of 1804.83Da, as determined by MALDI-TOF mass spectrometry. It was named Hejiang trypsin inhibitor (HJTI) in accord ...
DOI:
10.1016/j.peptides.2012.01.003

19. Kasstasin: A novel potent vasoconstrictor peptide from the skin secretion of the African red-legged running frog, Kassina maculata

Author:
Zhou, Mei,   et al.   ; Li, Xiaohe; Feng, Wanyu; Ma, Chengbang; Chen, Tianbao; Zeller, Martin; Hornshaw, Martin; Wang, Lei; Shaw, Chris;     Show all 9 Authors
Source:
Biochimie 2011 v.93 no.9 pp. 1537-1542
ISSN:
0300-9084
Subject:
amino acids; bioactive properties; complementary DNA; frogs; molecular cloning; peptides; rats; secretion; smooth muscle; spectrometers; tail
Abstract:
... Amphibian skin secretions are established sources of bioactive peptides. Here we describe the isolation, structural and pharmacological characterisation of a novel vasoconstrictor peptide from the skin secretion of the African hyperoliid frog, Kassina maculata, which exhibits no structural similarity to any known class of amphibian skin peptide. The peptide consists of 21 amino acid residues, FIKE ...
DOI:
10.1016/j.biochi.2011.05.009

20. Kassorins: Novel innate immune system peptides from skin secretions of the African hyperoliid frogs, Kassina maculata and Kassina senegalensis

Author:
Zhou, Mei,   et al.   ; Chen, Hang; Wang, Lei; Zeller, Martin; Hornshaw, Martin; Wu, Youjia; Li, Jia; Hang, Xinxing; Cai, Jiqun; Chen, Tianbao; Shaw, Chris;     Show all 11 Authors
Source:
Molecular immunology 2011 v.48 no.4 pp. 442-451
ISSN:
0161-5890
Subject:
Staphylococcus aureus; antibacterial properties; chemotaxis; complementary DNA; frogs; histamine; horses; innate immunity; mast cells; muscle contraction; open reading frames; peptides; prototypes; rats; smooth muscle; surfactants; sweat
Abstract:
... From defensive skin secretions acquired from two species of African hyperoliid frogs, Kassina maculata and Kassina senegalensis, we have isolated two structurally related, C-terminally amidated tridecapeptides of novel primary structure that exhibit a broad spectrum of biological activity. In reflection of their structural novelty and species of origin, we named the peptides kassorin M (FLEGLLNTVT ...
DOI:
10.1016/j.molimm.2010.09.018