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1. Barley yellow dwarf viruses

Author:
Domier, L.L.
Source:
Desk encyclopedia of plant and fungal virology / editors-in-chief, Brian W.J. Mahy and Marc H.V. Van Regenmortel pp. -
Subject:
Barley yellow dwarf virus; plant viruses; phylogeny; virion; viral proteins; gene expression regulation; host range; disease transmission; virus replication; host-pathogen relationships; epidemiological studies; disease diagnosis; disease control
Handle:
10113/37366

2. Cloning of Red Clover and Alfalfa Polyphenol Oxidase Genes and Expression of Active Enzymes in Transgenic Alfalfa

Author:
Sullivan, Michael; Thoma, Sharon; Samac, Deborah; Hatfield, Ronald
Source:
Molecular breeding of forage and turf : the proceedings of the 3rd International Symposium on the Molecular Breeding of Forage and Turf / edited by A. Hopkins, [et al.] pp. -
Subject:
Trifolium pratense; Medicago sativa; alfalfa; catechol oxidase; molecular cloning; transgenic plants; proteolysis; conserved sequences; complementary DNA; enzyme activity; substrate specificity; gene expression regulation
Abstract:
... Red clover contains high levels of polyphenol oxidase (PPO) activity and o-diphenol substrates. This results in a characteristic post-harvest browning reaction associated with decreased protein degradation during ensiling. To define PPO's role in inhibition of post-harvest proteolysis, we are taking both biochemical and molecular approaches. We have cloned three unique PPO cDNAs from red clover le ...
Handle:
10113/43872

3. Computational integration of structural and functional genomics data across species to develop information on the porcine inflammatory gene regulatory pathway

Author:
Tuggle, C.K.; Wang, Y.F.; Couture, O.P.; Qu, L.; Uthe, J.J.; Kuhar, D.; Lunney, J.K.; Nettleton, D.; Dekkers, J.C.; Bearson, S.M.D.
Source:
Animal genomics for animal health : Paris, France, 25-27 October 2007 / volume editors, Marie-Hélène Pinard ... [et al.] ; proceedings of an international symposium organized by Agricultural Research Services (ARS) ... [et al.] pp. -
Subject:
transcription factors; messenger RNA; immunogenetics; swine diseases; microarray technology; Salmonella Typhimurium; inflammation; gene expression; salmonellosis; genes; genomics; immune response; lymph nodes; gene expression regulation; swine
Handle:
10113/20559

4. Ecology, development and gene regulation in Aspergillus flavus

Author:
Payne, Gary A.; Yu, Jiujiang
Source:
Aspergillus : molecular biology and genomics / edited by Masayuki Machida and Katsuya Gomi pp. -
Subject:
Aspergillus flavus; gene expression; gene expression regulation; microbial genetics; microbial growth; cell differentiation; microbial ecology
Handle:
10113/38763

5. Engineering new crops for safe castor oil production

Author:
Chen, G.Q.; Ahn, Y.J.; Vang, L.
Source:
Biotechnology and sustainable agriculture 2006 and beyond : proceedings of the 11th IAPTC&B Congress, August 31 [i.e.13] -18, 2006, Beijing, China / edited by Zhihong Xu ... [et al.] pp. -
Subject:
ricin; protein synthesis; albumins; seeds; antisense DNA; transgenic plants; Ricinus communis; gene silencing; genetic engineering; seed development
Handle:
10113/15041

6. Epigenetic regulation in bovine cells: nutrient-induced modulation of gene expression and cellular functions

Author:
Li, Congjun; Elsasser, Ted H.; Li, Robert W.
Source:
Animal genetics / Leopold J. Rechi, editor pp. -
Subject:
cattle; epigenetics; gene expression; gene expression regulation; ruminant nutrition; butyric acid; messenger RNA; microarray technology; cell cycle; immune response; signal transduction; cell growth; insulin-like growth factor II
Handle:
10113/44057

7. Expression of metallothionein in the liver of rats fed copper-deficient AIN-93G diet

Author:
Kang, Y.J.; Saari, J.T.
Source:
Trace elements in man and animals--9 : proceedings of the Ninth International Symposium on Trace Elements in Man and Animals / editors P.W.F. Fishcher ... [et al.] pp. -
Subject:
feeds; feed composition; copper; dietary minerals; nutrient deficiencies; liver; metallothionein; messenger RNA; gene expression regulation; transcription (genetics); hematocrit; hemoglobin; rats
Handle:
10113/22889

8. Functional genomic approaches for the study of fetal/placental development in swine with special emphasis on imprinted genes

Author:
Bischoff, S.R.; Tsai, S.; Hardison, N.; Motsinger-Reif, A.A.; Freking, B.A.; Piedrahita, J.A.
Source:
Control of pig reproduction VIII : proceedings of the Eighth International Conference on Pig Reproduction, Alberta, Canada, June 2009 / edited by H. Rodriguez-Martinez, J.L. Vallet and A.J. Ziecik pp. -
Subject:
swine; fetal development; placenta; genomic imprinting; evolution; genomics; gene expression regulation; microarray technology; sequence analysis; single nucleotide polymorphism; cluster analysis; messenger RNA
Handle:
10113/46887

9. Luteoviruses

Author:
Domier, L.L.; D'Arcy, C.J.
Source:
Desk encyclopedia of plant and fungal virology / editors-in-chief, Brian W.J. Mahy and Marc H.V. Van Regenmortel pp. -
Subject:
Luteovirus; plant viruses; phylogeny; virion; viral proteins; gene expression regulation; host range; disease transmission; virus replication; host-pathogen relationships; epidemiological studies; disease diagnosis
Handle:
10113/37367

10. Microarray data analysis using machine learning methods

Author:
Ressom, Habtom W.; Lakshman, Dilip; Yun, Song Joong; Pramanik, Saroj K.; De los Reyes, Benildo G.
Source:
Biosystems engineering / Ahindra Nag pp. -
Subject:
oligonucleotides; complementary DNA; microarray technology; bioinformatics; computer analysis; gene expression; gene expression regulation; algorithms
Handle:
10113/34772

11. Molecular mechanisms involved in biofilm formation by food-associated bacteria

Author:
Smith, James; Fratamico, Pina; Uhlich, Gaylen
Source:
Biofilms in the food and beverage industries / edited by Pina M. Fratamico, Bassam A. Annous and Nereus W. Gunther IV pp. -
Subject:
Cronobacter sakazakii; food pathogens; biofilm; quorum sensing; signal transduction; gene expression regulation; transcription (genetics); Salmonella enterica; Escherichia coli; Listeria monocytogenes; Yersinia pestis; Yersinia pseudotuberculosis; Yersinia enterocolitica; Staphylococcus aureus; Staphylococcus epidermidis; Bacillus cereus; Clostridium difficile; Clostridium perfringens; Clostridium botulinum; Vibrio cholerae; Vibrio vulnificus; Shigella; Aeromonas hydrophila; Helicobacter pylori; Klebsiella pneumoniae; Serratia marcescens; genomics; proteomics
Handle:
10113/37639

12. Phytohormone regulation of cotton fiber development in vitro

Author:
Triplett, B.A.; Kim, H.J.; Hinchliffe, D.; Sze, S.H.; Thaxton, P.; Stelly, D.; Chen, Z.J.
Source:
Biotechnology and sustainable agriculture 2006 and beyond : proceedings of the 11th IAPTC&B Congress, August 31 [i.e.13] -18, 2006, Beijing, China / edited by Zhihong Xu ... [et al.] pp. -
Subject:
cotton; ovule culture; hexosyltransferases; plant development; messenger RNA; biosynthesis; microarray technology; Gossypium hirsutum; trichomes; gibberellic acid; expressed sequence tags; cellulose; gene expression; auxins; cDNA libraries; gene expression regulation; promoter regions
Handle:
10113/14746

13. Regulation of Aspergillus flavus aflatoxin biosynthesis and development

Author:
Cary, Jeffrey W.; Szerszen, Leanne; Calvo, Ana M.
Source:
Mycotoxin prevention and control in agriculture / Michael Appell, editor ; David F. Kendra, editor ; Mary W. Trucksess, editor ; sponsored by the ACS Division of Agriculture and Food Chemistry pp. -
Subject:
Aspergillus nidulans; biosynthesis; aflatoxins; secondary metabolites; multigene family; gene expression regulation; virulence; Aspergillus flavus
Handle:
10113/46772

14. Restrained stress-induced heat shock protein (HSP70) expression in aortas and adrenal glands of moderately zinc-deficient rats

Author:
Momcilovic, B.; Blake, M.J.; Buckley, A.R.
Source:
Trace elements in man and animals : TEMA 8 : Proceedings of the Eighth International Symposium on Trace Elements in Man and Animals / editors M. Anke, D. Meissner & C.F. Mills pp. -
Subject:
restraint of animals; animal stress; animal welfare; zinc; nutrient deficiencies; animal disease models; rats; aorta; heat shock proteins; gene expression regulation; human health
Handle:
10113/46236

15. Transcriptional response of chicken macrophages to Salmonella enterica serovar enteritidis infection

Author:
Zhang, S.; Lillehoj, H.S.; Kim, C.H.; Keeler, C.L. Jr.; Babu, U.; Zhang, M.Z.
Source:
Animal genomics for animal health : Paris, France, 25-27 October 2007 / volume editors, Marie-Hélène Pinard ... [et al.] ; proceedings of an international symposium organized by Agricultural Research Services (ARS) ... [et al.] pp. -
Subject:
chickens; messenger RNA; Salmonella Enteritidis; microarray technology; macrophages; poultry diseases; transcription (genetics); gene expression; salmonellosis; gene expression regulation; chemokines
Handle:
10113/20563

16. Transcriptional responses to water stress and recovery in a drought-tolerant fescue wild grass (Festuca ovina; Poaceae)

Author:
Fan Qiu; Seton Bachle; Ryan Estes; Melvin R. Duvall; Jesse B. Nippert; Mark C. Ungerer
Source:
Genome 0000 v.64 no.1 pp. 15-27
ISSN:
1480-3321
Subject:
Festuca ovina; drought tolerance; gene expression regulation; grasses; plant growth; transcription (genetics); transcriptomics; water stress
Abstract:
... Water stress associated with drought-like conditions is a major factor limiting plant growth and impacts productivity of natural plant communities and agricultural crops. Molecular responses of plants to water stress have been studied most extensively in model species and crops, few of which have evolved natural drought tolerance. In the current study, we examined physiological and transcriptomic ...
DOI:
10.1139/gen-2020-0055

17. The Genetic Induction of Autotetraploidy: A Proposal for its Use in Corn Breeding

Author:
D. E. Alexander
Source:
Agronomy journal 1957 v.49 no.1 pp. 40-43
ISSN:
0002-1962
Subject:
agronomy; aneuploidy; autotetraploidy; corn; gene expression regulation; hybrids; inbreeding; seed set
Abstract:
... SynopsisA method for establishing autotetraploid synthetic varieties of maize by genetic means is outlined. The ultimate use of these synthetic varieties as a source of breeding material is proposed. If genetic inbalance is largely responsible for low seed set in maize tetraploids, fertile inbreds or fertile hybrid combinations can be expected to be isolated from synthetic varieties that are genet ...
DOI:
10.2134/agronj1957.00021962004900010010x

18. Phylloclade development in the Asparagaceae: an example of homoeosis

Author:
COONEY-SOVETTS, CLAIRE; SATTLER, ROLF
Source:
Botanical journal of the Linnean Society 1987 v.94 no.3 pp. 327-371
ISSN:
0024-4074
Subject:
Asparagus densiflorus; Danae racemosa; Ruscus aculeatus; gene expression regulation; leaves; shoots; stems
Abstract:
... Phylloclades are traditionally defined as flattened, determinate, leaf-like stems primarily on the basis of their axillary position. However, because the literature is replete with controversy over the morphological interpretation of these organs, a study of phylloclade development in comparison with leaf and stem development was undertaken in four closely related species of the Asparagaceae: Rusc ...
DOI:
10.1111/j.1095-8339.1986.tb01053.x

19. Genetic elements inducing gene changes at the pallida locus of Antirrhinum majus

Author:
Aslam, K.M.
Source:
Theoretical and applied genetics 1990 v.79 no.4 pp. 475-480
ISSN:
0040-5752
Subject:
Antirrhinum majus; crossing; alleles; loci; gene expression; corolla; pigmentation; genotype; mutation; phenotype; transposons; gene expression regulation
DOI:
10.1007/BF00226156
PubMed:
24226451

20. Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis

Author:
Twell, D.; Yamaguchi, J.; McCormick, S.
Source:
Development 1990 v.109 no.3 pp. 705-713
ISSN:
0950-1991
Subject:
Solanum lycopersicum var. lycopersicum; tomatoes; Nicotiana tabacum; tobacco; Arabidopsis thaliana; transgenic plants; pollen; promoter regions; gene expression regulation; microsporogenesis
Abstract:
... To investigate the regulation of gene expression during male gametophyte development, we analyzed the promoter activity of two different genes (LAT52 and LAT59) from tomato, isolated on the basis of their anther-specific expression. In transgenic tomato, tobacco and Arabidopsis plants containing the LAT52 promoter region fused to the beta-glucuronidase (GUS) gene, GUS activity was restricted to po ...
Handle:
10113/13445

21. The promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis

Author:
Smith, Timothy L.; Gaskell, Jill; Berka, Randy M.; Yang, Maria; Henner, Dennis J.; Cullen, Daniel
Source:
Gene 1990 v.88 no.2 pp. 259-262
Subject:
Aspergillus niger; glucan 1,4-alpha-glucosidase; promoter regions; transgenes; genetic transformation; gene expression regulation; transcription (genetics); Ustilago zeae
Abstract:
... Promoter sequences from the Aspergillus niger glucoamylase-encoding gene (glaA) were linked to the bacterial hygromycin (Hy) phosphotransferase-encoding gene (hph) and this chimeric marker was used to select Hy-resistant (hy(R)) Ustilago maydis transformants. This is an example of an Ascomycete promoter functioning in a Basidiomycete. Hy(R) transformants varied with respect to copy number of integ ...
Handle:
10113/32429
DOI:
10.1016/0378-1119(90)90040-X

22. Antisense inhibition of pectin esterase gene expression in transgenic tomatoes

Author:
Lisa N. Hall; Gregory A. Tucker; Christopher J.S. Smith; Colin F. Watson; Graham B. Seymour; Yvonne Bundick; Jeremy M. Boniwell; Jonathan D. Fletcher; John A. Ray; Wolfgang Schuch; Colin R. Bird; Don Grierson
Source:
plant journal 1993 v.3 no.1 pp. 121-129
ISSN:
0960-7412
Subject:
Solanum lycopersicum var. lycopersicum; antisense RNA; chimerism; complementary DNA; enzyme activity; esterification; fruiting; gene expression; gene expression regulation; genes; isozymes; leaves; messenger RNA; pectinesterase; pectins; ripening; roots; tomatoes; transgenic plants
Abstract:
... Tomato plants (Lycopersicon esculentum Mill. cv. Ailsa Craig) were transformed with chimaeric pectin esterase (PE; EC 3.1.1.11) antisense RNA genes, based on a cDNA isolated from a tomato fruit library. PE enzyme activity in ripe fruit of these plants was reduced by up to 93%, as a result of inhibition of PE mRNA accumulation from an endogenous gene, or genes. No major differences in fruit develop ...
DOI:
10.1111/j.1365-313X.1993.tb00015.x

23. Differential expression of the rol A plant oncogene and its effect on tobacco development

Author:
Mauro Carneiro; Françoise Vilaine
Source:
plant journal 1993 v.3 no.6 pp. 785-792
ISSN:
0960-7412
Subject:
binding sites; gene expression; gene expression regulation; internodes; leaves; messenger RNA; mutants; oncogenes; root growth; roots; tissues; tobacco; transgenic plants
Abstract:
... The rolA gene not only provokes the abnormal traits of wrinkled leaves, shortened internodes, and deficient root growth in tobacco, but also induction of roots in leaf disks in hormone‐free medium. The rolA mRNA is most abundant in the stem of transgenic tobacco, accumulating at fivefold and 50‐fold lower levels in the leaf and the root, respectively. Promoter deletion mutants were constructed and ...
DOI:
10.1111/j.1365-313X.1993.00785.x

24. Light-regulated and endogenous fluctuations of chloroplast transcript levels in Chlamydomonas. Regulation by transcription and RNA degradation

Author:
Salvador, Maria L.; Klein, Uwe; Bogorad, Lawrence
Source:
plant journal 1993 v.3 no.2 pp. 213-219
ISSN:
0960-7412
Subject:
in vivo studies; flowering; cell culture; scotophase; genes; Chlamydomonas reinhardtii; Angiospermae; RNA; gene expression regulation
Abstract:
... Changes in the relative sizes of pools of transcripts of organelle genes during plastid development are common in flowering plants, but technical difficulties have prevented direct determinations of the effects of changes in rates of transcription and degradation on such fluctuations. It has been possible to follow both rates in Chlamydomonas reinhardtii. In synchronous or asynchronous cultures of ...
DOI:
10.1046/j.1365-313X.1993.t01-13-00999.x
PubMed:
8220443

25. Regulation of the expression of rbcS and other photosynthetic genes by carbohydrates: a mechanism for the ‘sink regulation’ of photosynthesis?

Author:
Anne Krapp; Bettina Hofmann; Christian Schäfer; Mark Stitt
Source:
plant journal 1993 v.3 no.6 pp. 817-828
ISSN:
0960-7412
Subject:
Chenopodium; apoplast; beta-fructofuranosidase; carbohydrate content; carbon dioxide; cell suspension culture; gene expression; gene expression regulation; genes; glucose; glycolysis; leaves; methionine; phloem; photosynthesis; potatoes; radionuclides; ribosomal RNA; ribulose-bisphosphate carboxylase; sulfur; tobacco; transgenic plants
Abstract:
... These experiments were carried out to investigate whether accumulation of carbohydrate leads to decreased expression of genes involved in photosynthesis. Addition of glucose to autotrophic cell suspension cultures of Chenopodium led to a large and reversible decrease of the steady state transcript levels of rbcS, cab and atp‐& within 5 h, but did not decrease 18S rRNA or transcript for two glycoly ...
DOI:
10.1111/j.1365-313X.1993.00817.x

26. Repression of the high-methionine zein gene in the maize inbred line Mo17

Author:
Schickler, Hedva; Benner, Michael S.; Messing, Joachim
Source:
plant journal 1993 v.3 no.2 pp. 221-229
ISSN:
0960-7412
Subject:
corn; gene expression regulation; genes; inbred lines; messenger RNA; mutation; seeds; transcription factors; zein
Abstract:
... The methionine content in maize kernels is largely determined by the level of a methionine-rich 10 kDa protein, encoded by the gene Zps10/(22). In the maize inbred BSSS53 this gene is over-expressed compared with its normal expression in inbred lines like W64A and W22. This is in part due to the trans-acting factor Zpr10/(22). However, compared with the normal level of expression, this gene is und ...
DOI:
10.1046/j.1365-313X.1993.t01-14-00999.x

27. The MsK family of alfalfa protein kinase genes encodes homologues of shaggy/glycogen synthase kinase‐3 and shows differential expression patterns in plant organs and development

Author:
Aniko Pay; Claudia Jonak; Laszlo Bögre; Irute Meskiene; Theresia Mairinger; Aladar Szalay; Erwin Heberle‐Bors; Heribert Hirt
Source:
plant journal 1993 v.3 no.6 pp. 847-856
ISSN:
0960-7412
Subject:
Drosophila; alfalfa protein; cell communication; gene expression regulation; genes; glycogen (starch) synthase; plant organs; protein-serine-threonine kinases; rats; serine; threonine
Abstract:
... This paper reports on the isolation of a novel class of plant serine/threonine protein kinase genes, MsK‐1, MsK‐2 and MsK‐3. They belong to the superfamily of cdc2‐like genes, but show highest identity to the Drosophila shaggy and rat GSK‐3 proteins (65–70%). All of these kinases share a highly conserved catalytic protein kinase domain. Different amino‐terminal extensions distinguish the different ...
DOI:
10.1111/j.1365-313X.1993.00847.x

28. Transcription of two members of a gene family encoding phenylalanine ammonia‐lyase leads to remarkably different cell specificities and induction patterns

Author:
Diane Shufflebottom; Keith Edwards; Wolfgang Schuch; Michael Bevan
Source:
plant journal 1993 v.3 no.6 pp. 835-845
ISSN:
0960-7412
Subject:
Arabidopsis; beta-glucuronidase; biosynthesis; gene expression; gene expression regulation; green beans; messenger RNA; phenylalanine ammonia-lyase; plant development; potatoes; reporter genes; tissues; tobacco; transcription (genetics)
Abstract:
... Phenylalanine ammonia‐lyase (PAL) catalyses the first committed step in the biosynthesis of phenyl‐propanoids, which perform a variety of functions in plant development and in their interactions with the environment. French bean contains a small family of genes encoding PAL and two of these genes, PAL2 and PAL3, have been shown to be differentially expressed at the mRNA level in bean tissues. The ...
DOI:
10.1111/j.1365-313X.1993.00835.x

29. Cytokinins modulate stress response genes in isopentenyl transferase‐transfornied Nicotiana plumbaginifolia plants

Author:
S. A. Harding; A. C. Smigocki
Source:
Physiologia plantarum 1994 v.90 no.2 pp. 327-333
ISSN:
0031-9317
Subject:
Drosophila melanogaster; Nicotiana plumbaginifolia; clones; complementary DNA; cytokinins; gene expression; gene expression regulation; genes; heat stress; leaves; messenger RNA; polypeptides; proteins; stress response
Abstract:
... The effects of transiently elevated cytokinin levels on gene expression following stress were examined in transgenic Nicotiana plumbaginifolia plants. Plants were transformed with a bacterial gene encoding isopentenyl transferase (ipt) cloned behind the heat shock (HS) protein 70 promoter from Drosophila melanogaster. Following a l‐h, 45°C HS of whole plants, the ipt transcript levels in leaves in ...
DOI:
10.1111/j.1399-3054.1994.tb00395.x

30. Expression of maize KNOTTED1 related homeobox genes in the shoot apical meristem predicts patterns of morphogenesis in the vegetative shoot

Author:
Jackson, D.; Veit, B.; Hake, S.
Source:
Development 1994 v.120 no.2 pp. 405-413
ISSN:
0950-1991
Subject:
shoot meristems; apical meristems; morphogenesis; plant proteins; homeodomain proteins; homeotic genes; gene expression regulation
Abstract:
... In this paper we describe the expression patterns of a family of homeobox genes in maize and their relationship to organogenic domains in the vegetative shoot apical meristem. These genes are related by sequence to KNOTTED1, a gene characterized by dominant neomorphic mutations which perturb specific aspects of maize leaf development. Four members of this gene family are expressed in shoot meriste ...
Handle:
10113/94

31. Green Fluorescent Protein as a Marker for Gene Expression

Author:
Chalfie, M.; Euskirchen, G.; Ward, W.W.; Prasher, D.C.
Source:
Science 1994 v.263 no.5148 pp. 802-805
ISSN:
0036-8075
Subject:
Aequorea victoria; green fluorescent protein; biomarkers; gene expression regulation
Abstract:
... A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms. ...
DOI:
10.1126/science.8303295
PubMed:
8303295

32. Activity of plasma membrane H+-ATPase and expression of PMA1 and PMA2 genes in Saccharomyces cerevisiae cells grown at optimal and low pH

Author:
Carmelo, V.; Bogaerts, P.; Sa-Correia, I.
Source:
Archives of microbiology 1996 v.166 no.5 pp. 315-320
ISSN:
0302-8933
Subject:
H-transporting ATP synthase; Saccharomyces cerevisiae; adenosinetriphosphatase; gene expression; gene expression regulation; genes; hydrochloric acid; immunoassays; messenger RNA; pH; plasma membrane; yeasts
Abstract:
... Cells of Saccharomyces cerevisiae grown in media with an initial pH of 2.5-6.0, acidified with a strong acid (HCl), exhibited the highest plasma membrane H+-ATPase-specific activity at an initial pH of 6.0. At a lower pH (above pH 2.5) ATPase activity (62-83% of the maximum level) still allowed optimal growth. At pH 2.5. ATPase activity was about 30% of the maximum value and growth was impaired. Q ...
DOI:
10.1007/s002030050389
PubMed:
8929277

33. Expression of enzyme activities and transcripts required for nitrate assimilation in maize (Zea mays L. Al88 x BMS) suspension cell cultures

Author:
Redinbaugh, Margaret G.; Ritchie, Steven W.; Hendrix, K.W.; MacKown, Charles T.; Campbell, W.H.
Source:
Plant science 1996 v.119 no.1-2 pp. 169-181
Subject:
Zea mays; corn; cultured cells; culture media; nitrogen; nitrates; cell growth; assimilation (physiology); nitrate reductase (NADH); nitrite reductase; enzyme activity; transcription (genetics); gene expression regulation; cell suspension culture
Abstract:
... The response of a rapidly growing, transformable maize suspension cell culture line (Zea mays L. A188 x BMS) to changes in media N and NO3- treatment was examined. Growth, N content, enzyme activities and transcript levels were measured in cells transferred to N-rich media (N6) or to media lacking N (N-free). Transferring cells to N-free media had little impact on cell growth (fresh and dry weight ...
Handle:
10113/35839
DOI:
10.1016/0168-9452(96)04464-0

34. Glucose uptake occurs by facilitated diffusion in procyclic forms of Trypanosoma brucei

Author:
Wille, U.; Seyfang, A.; Duszenko, M.
Source:
European journal of biochemistry 1996 v.236 no.1 pp. 228-233
ISSN:
0014-2956
Subject:
Trypanosoma brucei; activation energy; blood flow; bloodstream forms; facilitated diffusion; gene expression regulation; glucose; glucose transporters; glycolysis; insects; isomers; monensin; ouabain; parasites; plasma membrane; procyclic forms; temperature
Abstract:
... The glucose transporter of Trypanosoma brucei procyclic forms was characterized and compared with its bloodstream form counterpart. Measuring the glucose consumption enzymatically, we determined a saturable uptake process of relatively high affinity (Km = 80 micromolar Vmax = 4 nmol mine-1 10(-8) cells), which showed substrate inhibition at glucose concentrations above 1.5 mM (Ki = 21 mM). Control ...
DOI:
10.1111/j.1432-1033.1996.00228.x
PubMed:
8617269

35. The Pectic Polysaccharide from Bupleurum falcatum L. Enhances Clearance of Immune Complexes in Mice

Author:
Tsukasa Matsumoto; Haruki Yamada
Source:
Phytotherapy research 1996 v.10 no.7 pp. 585-588
ISSN:
0951-418X
Subject:
Bupleurum falcatum; antigen-antibody complex; body weight; gene expression regulation; genes; immunoglobulin G; liver; messenger RNA; mice; mononuclear leukocytes; pectins; phagocytosis; receptors
Abstract:
... The effect of pectic polysaccharide, bupleuran 2IIb, on clearance of immune complex from circulation in vivo was examined in mice. The immune complex clearance was enhanced by intraperitoneal treatment of bupleuran 2IIb in a dose dependent fashion, whereas clearance of colloidal carbon was reduced by bupleuran 2IIb‐treatment. The body and organ weights were not significantly altered in bupleuran 2 ...
DOI:
10.1002/(SICI)1099-1573(199611)10:7<585::AID-PTR909>3.0.CO;2-M

36. Timing of gliotoxin biosynthesis in the fungal biological control agent Gliocladium virens (Trichoderma virens)

Author:
Wilhite, S.E.; Straney, D.C.
Source:
Applied microbiology and biotechnology 1996 v.45 no.4 pp. 513-518
ISSN:
0175-7598
Subject:
Trichoderma virens; antibiotics; biological control; biological control agents; biosynthesis; damping off; fungi; gene expression regulation; nutritional status
Abstract:
... Gliocladium virens is a filamentous fungus formulated for the biological control of damping-off diseases of plants. Part of its antagonistic activity is due to its production of an epidithiodiketopiperazine antibiotic, gliotoxin. A relatively short period of biocontrol activity limits the use of this biocontrol agent in certain applications. This report examines the apparent transient accumulation ...
DOI:
10.1007/s002530050721

37. Up-regulation of an adult cuticular gene by 20-hydroxyecdysone in insect metamorphosing epidermis cultured in vitro

Author:
Braquart, C.; Bouhin, H.; Quennedey, A.; Delachambre, J.
Source:
European journal of biochemistry 1996 v.240 no.2 pp. 336-341
ISSN:
0014-2956
Subject:
Tenebrio molitor; adults; ecdysterone; gene expression; gene expression regulation; genes; hormonal regulation; insects; metamorphosis; wings
Abstract:
... Pupal forewing epidermis of the Coleoptera, Tenebrio molitor, was used to develop an in vitro system to study the hormonal control of metamorphosis at the cellular and molecular levels. Exposure to 1 micromolar 20-hydroxyecdysone for 48 h caused the formation of a typical adult cuticle. Under these conditions the expression of ACP-20, an adult-specific cuticular gene, was fivefold higher than in a ...
DOI:
10.1111/j.1432-1033.1996.0336h.x
PubMed:
8841396

38. AZT blocks down-regulation of IL-2 and IFN-γ gene expression in HIV acutely infected cells

Author:
J. Fan; P. Li; T.-W. Kok; C. J. Burrell
Source:
Archives of virology 1997 v.142 no.5 pp. 1035-1043
ISSN:
0304-8608
Subject:
T-lymphocytes; actin; gene expression; gene expression regulation; interferon-gamma; interleukin-2; messenger RNA; reverse transcription
Abstract:
... HIV infection causes dysregulation of cytokine gene expression in CD4⁺ T cells of the infected host. Azidothymidine (AZT) inhibits HIV repli-cation by blocking reverse transcription. Using a one-stop cell-to-cell HIV infection model, we have investigated the expression of several key cytokines in HIV infected T cells in the absence or presence of AZT treatment. Acute HIV infection of T cells resul ...
DOI:
10.1007/s007050050139

39. Bean yellow mosaic virus coat protein and gusA gene expression in transgenic Gladiolus plants

Author:
Kamo, Kathryn
Source:
Acta horticulturae 1997 v. no.447 pp. 393-399
ISSN:
0567-7572
Subject:
Bean yellow mosaic virus; plant viruses; coat proteins; transgenes; Gladiolus; transgenic plants; reporter genes; gene expression regulation
Abstract:
... Transgenic plants of Gladiolus were created following particle gun bombardment with either the bean yellow mosaic virus coat protein or gusA gene each under control of the CaMV 35S promoter. Monoclonal antibodies were used in ELISA assays to detect expression of the bean yellow mosaic virus coat protein in leaves of the transgenic plants. The bean yellow mosaic virus coat protein was expressed in ...
Handle:
naldc:42400

40. Characterization of a tobacco extensin gene and regulation of its gene family in healthy plants and under various stress conditions

Author:
Cathy Hirsinger; Yves Parmentier; Andrée Durr; Jacqueline Fleck; Elisabeth Jamet
Source:
Plant molecular biology 1997 v.33 no.2 pp. 279-289
ISSN:
0167-4412
Subject:
DNA; Nicotiana sylvestris; Nicotiana tabacum; Nicotiana tomentosiformis; cell suspension culture; clones; crosslinking; extensin; gene expression regulation; genomic libraries; genomics; leaves; messenger RNA; molecular cloning; multigene family; plant damage; plant ovary; roots; seed germination; seeds; stems; tobacco
Abstract:
... A genomic clone (Ext 1.4) encoding an extensin was isolated from a Nicotiana tabacum genomic library. The encoded polypeptide showed features characteristic of extensins such as Ser-(Pro)4 repeats and a high content in Tyr and Lys residues. The presence of one Tyr-Leu-Tyr-Lys motif suggests the possibility for one intramolecular isodityrosine cross-link whereas numerous Val-Tyr-Lys motifs may part ...
DOI:
10.1023/A:1005738815383

41. Coordinate control and variation in X-linked gene expression among female mice

Author:
Alex D. Greenwood; E. Michelle Southard-Smith; Andrzej T. Galecki; David T. Burke
Source:
Mammalian genome 1997 v.8 no.11 pp. 818-822
ISSN:
0938-8990
Subject:
RNA; X chromosome; alleles; animal age; embryogenesis; females; gene expression; gene expression regulation; longevity; mice; variance
Abstract:
... In normal female mammals, one of the two X Chromosome (Chr) homologs per cell is silenced coordinately during early embryogenesis. The genes located on the inactivated X homolog are predicted to be influenced by the same underlying repression mechanism. To test the uniformity of cis-acting gene repression, 32 genetically identical F₁ female mice were analyzed for differential expression of homolog ...
DOI:
10.1007/s003359900585

42. Differential Levels of mRNAs for Cytokines, the Interleukin-2 Receptor and Class II DR/DQ Genes in Ovine Interstitial Pneumonia Induced by Maedi Visna Virus Infection

Author:
C. J. Woodall; L. J. Maclaren; N. J. Watt
Source:
Veterinary pathology v.34 no.3 pp. 204-211
ISSN:
1544-2217
Subject:
Visna maedi virus; animal pathology; gene expression regulation; genes; glycoproteins; granulocyte-macrophage colony-stimulating factor; interferon-gamma; interleukin-2; lungs; messenger RNA; pathogenesis; pneumonia; reverse transcriptase polymerase chain reaction; sheep; transforming growth factor alpha; transforming growth factor beta 1; tumor necrosis factor-alpha
Abstract:
... The relative levels of selected cytokine, interleukin-2 receptor, class II DR and DQ RNAs, and maedi visna virus (MVV) RNA were measured by reverse-transcriptase polymerase chain reaction (RT-PCR) in the lungs of sheep with natural maedi visna virus infection (n = 8) and a group of age/sex/breed-matched MVV seronegative sheep (n = 4). These animals were divided into two groups, irrespective of ser ...
DOI:
10.1177/030098589703400305
PubMed:
9163876

43. Differential expression of three Arabidopsis genes encoding the B' regulatory subunit of protein phosphatase 2A

Author:
Latorre, K.A.; Harris, D.M.; Rundle, S.J.
Source:
European journal of biochemistry 1997 v.245 no.1 pp. 156-163
ISSN:
0014-2956
Subject:
Arabidopsis thaliana; animals; cDNA libraries; clones; complementary DNA; enzyme activity; gene expression regulation; genes; heat shock response; messenger RNA; metabolism; nucleotide sequences; polymerase chain reaction; polypeptides; protein subunits; signal transduction; yeasts
Abstract:
... Numerous plant processes ranging from signal transduction to metabolism appear to be mediated, in part, by type 2A protein serine/threonine phosphatases (PP2A). In an effort to identify factors that control the activity of this enzyme in plants, we have isolated and characterized DNA sequences encoding the B' regulatory subunit of PP2A from Arabidopsis thaliana. Specifically, we used PCR to amplif ...
DOI:
10.1111/j.1432-1033.1997.00156.x
PubMed:
9128737

44. Effect of ion pairing on the cellular transport of antisense oligonucleotide

Author:
Kyung Song; Kyoung Mi Kim; Jaebaek Kim; Geonil Ko; Dong Hwan Sohn
Source:
Archives of pharmacal research 1997 v.20 no.5 pp. 438-442
ISSN:
0253-6269
Subject:
benzalkonium chloride; cations; dose response; gene expression regulation; genes; macrophages; mice; neutralization; nitric oxide; nitrites; oligonucleotides; physiological transport; transforming growth factor beta; tumor necrosis factor-alpha
Abstract:
... Antisense oligonucleotide represents an interesting tool for selective inhibition of gene expression. However, their low efficiency of introduction within intact cells remains to be overcome. Antisense-TGFβ (25 mer) and antisense-TNFα (18 mer) were used to study the cellular transport and biological function of antisense oligonucleotide in vitro. Since TGF and TNF play on important role in regulat ...
DOI:
10.1007/BF02973936

45. Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces

Author:
Wymelenberg, A.J. vanden; Cullen, D.; Spear, R.N.; Schoenike, B.; Andrews, J.H.
Source:
Biotechniques 1997 v.23 no.4 pp. 686-690
ISSN:
0736-6205
Subject:
Aureobasidium pullulans; plant pathogenic fungi; green fluorescent protein; genetic transformation; gene expression regulation; host-pathogen relationships
Abstract:
... A red-shifted, mutated form of the jelly-fish green fluorescent protein (GFP) under control of a TEF promoter was expressed at high levels in the filamentous fungus Aureobasidium pullulans. In the three transformants studied, all morphotypes of the fungus, including pigmented chlamydospores, expressed GFP and fluoresced brightly. Confocal microscopy showed that the intra-cellular distribution of G ...
Handle:
10113/19138

46. Expression of the feline herpesvirus type 1 ICP4 gene is controlled by two alternative promoters

Author:
Y. Kawaguchi; K. Maeda; N. Yokoyama; K. Fujita; M. Ono; Y. Tohya; T. Mikami
Source:
Archives of virology 1997 v.142 no.2 pp. 239-253
ISSN:
0304-8608
Subject:
messenger RNA; Human alphaherpesvirus 1; Felid alphaherpesvirus 1; genes; gene expression regulation; promoter regions
Abstract:
... Feline herpesvirus type 1 (FHV-1) produces a single 5.4 kb immediate-early (IE) transcript encoding FHV-1 ICP4 which acts as a trans-acting factor [Kawaguchi et al. (1994) Virology 204: 430–435]. Our earlier study has shown that the FHV-1 IE transcript is spliced in the leader region and the FHV-1 IE gene product (ICP4) down-regulates its own promoter through the region which includes its transcri ...
DOI:
10.1007/s007050050074
PubMed:
9125041

47. High mobility group proteins cHMG 1a, cHMG 1b, and cHMGI are distinctly distributed in chromosomes and differentially expressed during ecdysone dependent cell differentiation

Author:
Sonja Ghidelli; Peter Claus; Georg Thies; Jacek R. Wiśniewski
Source:
Chromosoma 1997 v.105 no.6 pp. 369-379
ISSN:
0009-5915
Subject:
DNA; Diptera; cell differentiation; cell nucleolus; chromatin; ecdysone; fluorescent antibody technique; gene expression regulation; genes; interphase; mammals; transcription (genetics)
Abstract:
... The mammalian high mobility group proteins HMGI/Y and HMG1/2 are thought to play an architectural role in assembly of nucleoprotein structures. Counterparts to these proteins have recently been found in the cells of the Dipteran insectChironomus. In this report we investigate the distribution of three abundant HMG proteins in interphase giant chromosomes of the midge,Chironomus. By means of the in ...
DOI:
10.1007/BF02529752
PubMed:
9087379

48. Molecular characterization of glyoxalase II from Arabidopsis thaliana

Author:
Mrinal K. Maiti; Subbiah Krishnasamy; Heather A. Owen; Christopher A. Makaroff*
Source:
Plant molecular biology 1997 v.35 no.4 pp. 471-481
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; Escherichia coli; Southern blotting; buds; cell differentiation; complementary DNA; cytotoxicity; gene expression regulation; isozymes; multigene family; mutants; proteins; roots; sequence analysis
Abstract:
... Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. During the analysis of a T-DNA-tagged mutant of Arabidopsis we identified the gene for ...
DOI:
10.1023/A:1005891123344

49. Mutations in myostatin (GDF8) in Double-Muscled Belgian Blue and Piedmontese Cattle

Author:
Kambadur, R.; Sharma, M.; Smith, T.P.L.; Bass, J.J.
Source:
Genome research 1997 v.7 no.9 pp. 910-915
ISSN:
1549-5469
Subject:
Belgian Blue; Piedmontese; muscular diseases; hypertrophy; cattle diseases; genetic disorders; transforming growth factor beta; mutation; complementary DNA; sequence analysis; conserved sequences; alleles; gene expression regulation
Abstract:
... A visibly distinct muscular hypertrophy (mh), commonly known as double muscling, occurs with high frequency in the Belgian Blue and Piedmontese cattle breeds. The autosomal recessive mh locus causing double-muscling condition in these cattle maps to bovine chromosome 2 within the same interval as myostatin, a member of the TGF-β superfamily of genes. Because targeted disruption of myostatin in mic ...
Handle:
10113/120
PubMed:
9314496

50. Oestradiol regulated keratin gene expression in rat vaginal epithelial cells

Author:
K. Sridhar Rao; A. G. Reddy; P. D. Gupta
Source:
Journal of biosciences 1997 v.22 no.3 pp. 273-285
ISSN:
0250-5991
Subject:
epithelial cells; estradiol; gene expression; gene expression regulation; genes; keratin; mechanism of action; mitogens; polypeptides; rats; transcription (genetics)
Abstract:
... Oestradiol acts both as a mitogen and as an inducer of differentiation of target cells. The cellular responses to oestradiol are generally mediated through the regulation of gene expression, although nongenomic modes of action are also documented. The present observations show that the regulation of keratin gene expression in rat vaginal epithelial cells is under the influence of oestradiol. It is ...
DOI:
10.1007/BF02703231