PubAg

Main content area

You searched for: Subject "immune response" Remove constraint Subject: "immune response" Subject genes Remove constraint Subject: genes Subject messenger RNA Remove constraint Subject: messenger RNA
Start Over

Search

804  Search Results

« Previous | 1 - 50 of 804 |
Number of results to display per page

Search Results

1. Drosophila Thor participates in host immune defense and connects a translational regulator with innate immunity

Author:
Bernal, A.; Kimbrell, D.A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2000 v.97 no.11 pp. 6019-6024
ISSN:
0027-8424
Subject:
immune response,   etc   ; transcription factors; Micrococcus; messenger RNA; Kocuria rosea; Staphylococcus epidermidis; binding proteins; gene expression; amino acid sequences; genes; binding sites; Enterobacter cloacae; Drosophila melanogaster; promoter regions; bacteria; Escherichia coli;     Show all 16 Subjects
DOI:
10.1073/pnas.100391597
PubMed:
10811906
PubMed Central:
PMC18551

2. Resveratrol inhibits intestinal tumorigenesis and modulates host-defense-related gene expression in an animal model of human familial adenomatous polyposis

Author:
Schneider, Y.; Duranton, B.; Gosse, F.; Schleiffer, R.; Seiler, N.; Raul, F.
Source:
Nutrition and cancer 2001 v.39 no.1 pp. 102-107
ISSN:
0163-5581
Subject:
immune response,   etc   ; carcinogenesis; messenger RNA; ileum; experimental diets; mice; gene expression; jejunum; animal models; intestinal mucosa; genes; cell proliferation; cell cycle; antioxidants; colon;     Show all 15 Subjects
Abstract:
... We studied the effect of oral administration of resveratrol, a natural constituent of grapes, on tumorigenesis in Min mice. Min mice are congenic mice genetically predisposed to develop intestinal tumors as a result of a mutation of the Apc gene. Resveratrol (0.01% in the drinking water containing 0.4% ethanol) was administered for seven weeks to Min mice starting at five weeks of age. The control ...
DOI:
10.1207/S15327914nc391_14
PubMed:
11588890

3. Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays

Author:
Gregorio, E. de.; Spellman, P.T.; Rubin, G.M.; Lemaitre, B.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2001 v.98 no.22 pp. 12590-12595
ISSN:
0027-8424
Subject:
immune response,   etc   ; messenger RNA; Micrococcus luteus; Beauveria bassiana; gene expression; genes; Drosophila melanogaster; bacteria; Escherichia coli;     Show all 9 Subjects
Abstract:
... To identify new Drosophila genes involved in the immune response, we monitored the gene expression profile of adult flies in response to microbial infection by using high-density oligonucleotide microarrays encompassing nearly the full Drosophila genome. Of 13,197 genes tested, we have characterized 230 induced and 170 repressed by microbial infection, most of which had not previously been associa ...
DOI:
10.1073/pnas.221458698
PubMed:
11606746
PubMed Central:
PMC60098

4. Altered expression of cellular genes in neutrophils of periparturient dairy cows

Author:
Madsen, Sally A.; Weber, Patty S.D.; Burton, Jeanne L.
Source:
Veterinary immunology and immunopathology 2002 v.86 no.3-4 pp. 159-175
ISSN:
0165-2427
Subject:
immune response,   etc   ; DNA-binding proteins; Northern blotting; animal health; blood serum; complementary DNA; cytochrome b; dairy cows; estradiol; gene expression; gene expression regulation; genes; mastitis; messenger RNA; metabolism; neutrophils; nucleic acid hybridization; nucleotide sequences; parturition; progesterone; radioimmunoassays; reverse transcriptase polymerase chain reaction; screening; sequence homology; steroid hormones; tricarboxylic acid cycle;     Show all 26 Subjects
Abstract:
... The periparturient dairy cow undergoes a plethora of physiological changes, including changes in the immune system that lead to profound effects on animal health. Of the immune cells affected at parturition, the neutrophil has been of particular interest due to its primary role in innate immune defense against mastitis. Immune functions of bovine neutrophils are known to be depressed around partur ...
DOI:
10.1016/S0165-2427(02)00020-X

5. Biotin Supplementation Increases Expression of Genes Encoding Interferon-γ, Interleukin-1β, and 3-Methylcrotonyl-CoA Carboxylase, and Decreases Expression of the Gene Encoding Interleukin-4 in Human Peripheral Blood Mononuclear Cells

Author:
Wiedmann, Silke; Eudy, James D.; Zempleni, Janos
Source:
Journal of nutrition 2003 v.133 no.3 pp. 716-719
ISSN:
0022-3166
Subject:
immune response,   etc   ; adults; antigens; apoptosis; biotin; cell proliferation; concanavalin A; cultured cells; gene expression; gene expression regulation; genes; homeostasis; humans; interferon-gamma; interleukin-4; messenger RNA; metabolism; microarray technology; mononuclear leukocytes; reverse transcriptase polymerase chain reaction; secretion; signal transduction; stress response;     Show all 23 Subjects
Abstract:
... Stimulation of immune cells by antigens triggers changes in the transcription of genes encoding cytokines and other proteins; these changes in gene expression are part of the normal immune response. Previous studies have provided evidence that biotin status may affect secretion of cytokines by immune cells. Here we determined whether biotin supplementation affects gene expression in human immune c ...

6. Regulation of Zinc Metabolism and Genomic Outcomes

Author:
Cousins, Robert J.; Blachard, Raymond K.; Moore, J. Bernadette; Cui, Li; Green, Calvert L.; Liuzzi, Juan P.; Cao, Jay; Bobo, Jeffrey A.
Source:
Journal of nutrition 2003 v.133 no.5 pp. 1521S-1526S
ISSN:
0022-3166
Subject:
immune response,   etc   ; DNA repair; cholecystokinin; complementary DNA; energy; genes; genomics; heat shock proteins; humans; kidneys; lactation; messenger RNA; metabolism; monocytes; pregnancy; signal transduction; small intestine; stress response; transcriptome; transporters; tyrosine; zinc;     Show all 22 Subjects
Abstract:
... Differential mRNA display and cDNA array analysis have identified zinc-regulated genes in small intestine, thymus and monocytes. The vast majority of the transcriptome is not influenced by dietary zinc intake, high or low. Of the genes that are zinc regulated, most are involved in signal transduction (particularly influencing the immune response), responses to stress and redox, growth and energy u ...

7. Simultaneous quantitation of equine cytokine mRNAs using a multi-probe ribonuclease protection assay

Author:
Lim, W.S.; Edwards, J.F.; Boyd, N.K.; Payne, S.L.; Ball, J.M.
Source:
Veterinary immunology and immunopathology 2003 v.91 no.1 pp. 45-51
ISSN:
0165-2427
Subject:
immune response,   etc   ; RNA probes; actin; antisense RNA; genes; horses; interferon-gamma; interleukin-10; interleukin-12; interleukin-1alpha; interleukin-1beta; interleukin-6; interleukin-8; macrophages; messenger RNA; mononuclear leukocytes; plasmids; radiolabeling; ribonucleases; tumor necrosis factor-alpha;     Show all 20 Subjects
Abstract:
... A rapid multi-probe ribonuclease protection assay (RPA) was developed to quantitate equine-specific cytokine mRNA levels in activated equine monocyte-derived macrophages (EMDM) and equine peripheral blood mononuclear cells (EPBMC). Eleven template plasmids specific to 10 equine cytokine genes and the β-actin gene were generated from which radiolabeled anti-sense RNA probes were produced. The multi ...
DOI:
10.1016/S0165-2427(02)00263-5

8. Use of recombinant modified vaccinia Ankara viral vectors for equine influenza vaccination

Author:
Breathnach, C.C.; Rudersdorf, R.; Lunn, D.P.
Source:
Veterinary immunology and immunopathology 2004 v.98 no.3-4 pp. 127-136
ISSN:
0165-2427
Subject:
immune response,   etc   ; Influenza A virus; antibodies; antigens; blood serum; equine influenza; genes; hemagglutinins; homologous recombination; horses; interferon-gamma; loci; messenger RNA; neck; nucleoproteins; pathogens; plasmids; vaccination; viruses;     Show all 19 Subjects
Abstract:
... Recombinant modified vaccinia Ankara (MVA) vectors expressing equine influenza virus genes were constructed and evaluated for use in equine vaccination. Two strains of recombinant MVA, expressing either hemagglutinin (HA) or nucleoprotein (NP) genes were constructed. Each influenza virus gene was cloned from A/equine/Kentucky/1/81 (Eq/Ky) into an MVA construction plasmid, and was introduced to the ...
DOI:
10.1016/j.vetimm.2003.11.004

9. Upregulation of transforming growth factor-beta and interleukin-10 in cows with clinical Johne’s disease

Author:
Khalifeh, M.S.; Stabel, J.R.
Source:
Veterinary immunology and immunopathology 2004 v.99 no.1-2 pp. 39-46
ISSN:
0165-2427
Subject:
immune response,   etc   ; Mycobacterium avium subsp. paratuberculosis; cows; diarrhea; disease course; gene expression; genes; ileum; interferon-gamma; interleukin-10; lymph nodes; messenger RNA; reverse transcriptase polymerase chain reaction; tissues; transforming growth factor beta; weight loss;     Show all 16 Subjects
Abstract:
... Johne’s disease progresses through distinct stages including a protracted subclinical stage in which the infection appears to be controlled; followed by a more acute stage in which the host animal demonstrates clinical signs such as diarrhea and weight loss. Little is known about the dynamics of the host immune response during these two phases of disease, however, it is possible that immune modula ...
DOI:
10.1016/j.vetimm.2004.01.009

10. Mucosal FOXP3-Expressing CD4⁺ CD25[superscript high] Regulatory T Cells in Helicobacter pylori-Infected Patients

Author:
Lundgren, Anna; Strömberg, Erika; Sjöling, Åsa; Lindholm, Catharina; Enarsson, Karin; Edebo, Anders; Johnsson, Erik; Suri-Payer, Elisabeth; Larsson, Pia; Rudin, Anna; Svennerholm, Ann-Mari; Lundin, B. Samuel
Source:
Infection and immunity 2005 v.73 no.1 pp. 523-531
ISSN:
0019-9567
Subject:
immune response,   etc   ; Helicobacter pylori; T-lymphocytes; adenocarcinoma; antigens; cytotoxicity; duodenum; genes; messenger RNA; mucosa; patients; peptic ulcers; phenotype; stomach;     Show all 14 Subjects
Abstract:
... Helicobacter pylori chronically colonizes the stomach and duodenum and causes peptic ulcers or gastric adenocarcinoma in 10 to 20% of infected individuals. We hypothesize that the inability of patients to clear H. pylori infections is a consequence of active suppression of the immune response. Here we show that H. pylori-infected individuals have increased frequencies of CD4⁺ CD25[superscript high ...
DOI:
10.1128/IAI.73.1.523-531.2005

11. Drosophila host defense after oral infection by an entomopathogenic Pseudomonas species

Author:
Vodovar, N.; Vinals, M.; Liehl, P.; Basset, A.; Degrouard, J.; Spellman, P.; Boccard, F.; Lemaitre, B.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2005 v.102 no.32 pp. 11414-11419
ISSN:
0027-8424
Subject:
immune response,   etc   ; Drosophila melanogaster; bacterial infections; entomopathogenic bacteria; Pseudomonas; ingestion; gene expression; messenger RNA; microarray technology; transcription (genetics); disease resistance; resistance mechanisms; nucleotide sequences; genes; ribosomal RNA; host-pathogen relationships; pathogenicity;     Show all 17 Subjects
Abstract:
... Drosophila has been shown to be a valuable model for the investigation of host-pathogen interactions. Study of the Drosophila immune response has been hampered, however, by the lack of true Drosophila pathogens. In nearly all studies reported, the bacteria used were directly injected within the body cavity of the insect, bypassing the initial steps of a natural interaction. Here, we report the ide ...
DOI:
10.1073/pnas.0502240102
PubMed:
16061818
PubMed Central:
PMC1183552

12. Immunization Enhances Inflammation and Tissue Destruction in Response to Porphyromonas gingivalis

Author:
Leone, Cataldo W.; Bokhadhoor, Haneen; Kuo, David; Desta, Tesfahun; Yang, Julia; Siqueira, Michelle F.; Amar, Salomon; Graves, Dana T.
Source:
Infection and immunity 2006 v.74 no.4 pp. 2286-2292
ISSN:
0019-9567
Subject:
immune response,   etc   ; Porphyromonas gingivalis; adaptive immunity; apoptosis; bone resorption; chemokines; fibroblasts; gene expression; genes; immunization; inflammation; innate immunity; interferon-gamma; interleukin-6; messenger RNA; mice; models; periodontal diseases; quantitative polymerase chain reaction;     Show all 19 Subjects
Abstract:
... It is well established that host-bacterium interactions play a critical role in the initiation and progression of periodontal diseases. By the use of inhibitors, it has been shown that mediators associated with the innate immune response significantly contribute to the disease process. Less is known regarding the role of the acquired immune response. To investigate mechanisms by which the acquired ...
DOI:
10.1128/IAI.74.4.2286-2292.2006
PubMed:
16552059
PubMed Central:
PMC1418897

13. Genomic organisation and expression of the natural killer cell enhancing factor (NKEF) gene in channel catfish, Ictalurus punctatus (Rafinesque)

Author:
Li, R.W.; Waldbieser, G.C.
Source:
Fish & shellfish immunology 2006 v.20 no.1 pp. 72
ISSN:
1050-4648
Subject:
immune response,   etc   ; Ictalurus punctatus; blood cells; animal proteins; genes; complementary DNA; nucleotide sequences; introns; exons; promoter regions; genetic polymorphism; amino acid sequences; chromosome mapping; gene expression; messenger RNA; alleles; animal tissues; lipopolysaccharides; Escherichia coli; immunity;     Show all 20 Subjects
Abstract:
... The gene encoding Natural Killer Cell Enhancing Factor (NKEF) was identified in clones from a gynogenetic channel catfish BAC library. NKEF gene sequence (5.2 kb) was obtained by direct sequencing of BAC clones. The catfish NKEF gene contains one non-coding exon, five coding exons and five introns. A putative TATA box and other transcription factor binding sites were identified in the promoter reg ...
Handle:
10113/215
DOI:
10.1016/j.fsi.2005.04.002
PubMed:
15967680

14. Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance

Author:
Sugimoto, M.; Fujikawa, A.; Womack, J.E.; Sugimoto, Y.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2006 v.103 no.17 pp. 6454-6459
ISSN:
0027-8424
Subject:
immune response,   etc   ; cattle; transcription factors; genes; nucleotide sequences; transcription (genetics); gene induction; bovine mastitis; Escherichia coli; lipopolysaccharides; gene expression; messenger RNA; mammary glands; interleukin-8; tumor necrosis factor-alpha; disease resistance;     Show all 16 Subjects
Abstract:
... Mastitis, a mammary gland inflammation in response to bacterial infection, is a major problem in the dairy industry. We found that cows susceptible to mastitis have a three-base insertion in a glycine-coding stretch of the gene for forebrain embryonic zinc finger-like (FEZL), a transcription factor with a role in neuronal development. Mastitis induces FEZL expression in mammary glands, and induced ...
DOI:
10.1073/pnas.0601015103
PubMed:
16611727
PubMed Central:
PMC1458905

15. Genomic features of Bordetella parapertussis clades with distinct host species specificity

Author:
Brinig, Mary M; Register, Karen B; Ackermann, Mark R; Relman, David A
Source:
Genome biology 2006 v.7 no.9 pp. 1349
ISSN:
1465-6906
Subject:
immune response,   etc   ; Bordetella parapertussis; strains; strain differences; sheep diseases; human diseases; microbial genetics; genome; nucleic acid hybridization; genes; microarray technology; gene expression; messenger RNA; bacterial colonization; respiratory tract diseases; nose; granulocytes; bacterial infections;     Show all 18 Subjects
Abstract:
... BACKGROUND: The respiratory pathogen Bordetella parapertussis is a valuable model in which to study the complex phenotype of host specificity because of its unique two-species host range. One subset of strains, including the sequenced representative, causes whooping cough in humans, while other strains infect only sheep. The disease process in sheep is not well understood, nor are the genetic and ...
Handle:
10113/29307
DOI:
10.1186/gb-2006-7-9-r81
PubMed:
16956413
PubMed Central:
PMC1794550

16. Identification and gene expression of the bovine C-type lectin Dectin-1

Author:
Willcocks, S.; Yamakawa, Y.; Stalker, A.; Coffey, T.J.; Goldammer, T.; Werling, D.
Source:
Veterinary immunology and immunopathology 2006 v.113 no.1-2 pp. 234-242
ISSN:
0165-2427
Subject:
immune response,   etc   ; cattle; lectins; receptors; gene expression; protein synthesis; outer membrane proteins; signal transduction; nucleotide sequences; amino acid sequences; chromosome mapping; isomers; messenger RNA; transcription (genetics); bone marrow; monocytes; macrophages; dendritic cells; natural killer cells; CD4-positive T-lymphocytes; B-lymphocytes; genes; genomics;     Show all 23 Subjects
Abstract:
... C-type lectin receptors (CTLR) are cell-surface signalling molecules that recognize a range of highly conserved pathogen molecules and instigate the appropriate immune response. Here, we report the cloning, sequencing, mapping and expression pattern of the bovine C-type lectin domain family 7, member A (CLEC7A; synonyms CLCSF12, Dectin-1). We identified two isoforms, similar to the human system, w ...
DOI:
10.1016/j.vetimm.2006.04.007

17. Comparative analysis of serine protease-related genes in the honey bee genome: possible involvement in embryonic development and innate immunity

Author:
Zou, Z.; Lopez, Dawn L.; Kanost, Michael R.; Evans, Jay D.; Jiang, Haobo
Source:
Insect molecular biology 2006 v.15 no.5 pp. 603
ISSN:
0962-1075
Subject:
immune response,   etc   ; Apis mellifera; honey bees; genome; serine proteinases; genes; amino acid sequences; sequence homology; phylogeny; embryo (animal); embryogenesis; insect immunity; gene expression; messenger RNA;     Show all 14 Subjects
Abstract:
... We have identified 44 serine protease (SP) and 13 serine protease homolog (SPH) genes in the genome of Apis mellifera. Most of these genes encode putative secreted proteins, but four SPs and three SPHs may associate with the plasma membrane via a transmembrane region. Clip domains represent the most abundant non-catalytic structural units in these SP-like proteins -12 SPs and six SPHs contain at l ...
Handle:
10113/28352
DOI:
10.1111/j.1365-2583.2006.00684.x
PubMed:
17069636
PubMed Central:
PMC1761132

18. Quantitation of immune response gene expression and cellular localisation of interleukin-1β mRNA in Atlantic salmon, Salmo salar L., affected by amoebic gill disease (AGD)

Author:
Bridle, A.R.; Morrison, R.N.; Cunningham, P.M.C.; Nowak, B.F.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 121-134
ISSN:
0165-2427
Subject:
immune response,   etc   ; Salmo salar; salmon; gene expression; quantitative analysis; interleukin-1; messenger RNA; protein synthesis; amebiasis; Amoebida; fish diseases; reverse transcriptase polymerase chain reaction; nucleic acid hybridization; genes; nitric oxide synthase; animal proteins; gills; liver; kidneys; gene expression regulation; infection;     Show all 21 Subjects
Abstract:
... The characterisation of selected immune response genes during amoebic gill disease (AGD) in Atlantic salmon, Salmo salar L., was performed using semi-quantitative RT-PCR, quantitative real-time RT-PCR (qRT-PCR), and in situ hybridisation (ISH). The immune response genes of interest were interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), serum amyloid A (SAA), and serum amyloid P-like ...
DOI:
10.1016/j.vetimm.2006.08.002

19. Comparison of the Human and Canine Cytokines IL-1(α/β) and TNF-α to Orthologous Other Mammalians

Author:
Soller, Jan T.; Murua-Escobar, Hugo; Willenbrock, Saskia; Janssen, Miriam; Eberle, Nina; Bullerdiek, Jörn; Nolte, Ingo
Source:
Journal of heredity 2007 v.98 no.5 pp. 485-490
ISSN:
0022-1503
Subject:
immune response,   etc   ; Alzheimer disease; Parkinson disease; amino acid sequences; cats; cattle; dog diseases; dogs; genes; genetic background; histiocytosis; horses; humans; inflammation; interleukin-1beta; messenger RNA; metabolic diseases; mice; models; necrosis; neoplasms; nucleotide sequences; pathogenesis; proteins; rats; rheumatoid arthritis; sheep; swine; tumor necrosis factor-alpha;     Show all 29 Subjects
Abstract:
... The cytokines interleukin-1 (IL-1α and IL-1β) and the tumor necrosis factor-α (TNF-α) both play a major role in the initiation and regulation of inflammation and immunity responses. Polymorphisms within the gene sequences of these cytokines IL-1 and TNF-α have been proposed to play an important role in the pathogenesis of certain diseases. Affecting nearly every organ, various diseases, including ...
DOI:
10.1093/jhered/esm025

20. Helicobacter pylori Induces CCL20 Expression

Author:
Tomimori, Koh; Uema, Eriko; Teruya, Hiromitsu; Ishikawa, Chie; Okudaira, Taeko; Senba, Masachika; Yamamoto, Kazuo; Matsuyama, Toshifumi; Kinjo, Fukunori; Fujita, Jiro; Mori, Naoki
Source:
Infection and immunity 2007 v.75 no.11 pp. 5223-5232
ISSN:
0019-9567
Subject:
immune response,   etc   ; Helicobacter pylori; T-lymphocytes; chemokines; dendritic cells; electrophoresis; epithelial cells; genes; humans; immunohistochemistry; inflammation; luciferase; messenger RNA; mutants; pathogenicity islands; reverse transcriptase polymerase chain reaction; signal transduction; transcription (genetics); transcription factors; transfection;     Show all 20 Subjects
Abstract:
... CCL20 attracts immature dendritic cells and memory T cells and plays a role on mucosal surfaces in inflammation. However, whether Helicobacter pylori infection induces CCL20 in human gastric epithelial cells remains to be determined. The aim of this study was to analyze the molecular mechanism of H. pylori-induced CCL20 expression. Expression of CCL20 mRNA was assessed by reverse transcription-PCR ...
DOI:
10.1128/IAI.00731-07
PubMed:
17724069
PubMed Central:
PMC2168315

21. Long-Term Staphylococcal Enterotoxin C1 Exposure Induces Soluble Factor-Mediated Immunosuppression by Bovine CD4⁺ and CD8⁺ T Cells

Author:
Seo, Keun Seok; Lee, Sang Un; Park, Yong Ho; Davis, William C.; Fox, Lawrence K.; Bohach, Gregory A.
Source:
Infection and immunity 2007 v.75 no.1 pp. 260-269
ISSN:
0019-9567
Subject:
immune response,   etc   ; Staphylococcus aureus; T-lymphocytes; cattle; gene expression regulation; genes; immune evasion; interleukin-10; interleukin-2; messenger RNA; pathogens; superantigens; transforming growth factor beta;     Show all 13 Subjects
Abstract:
... Regulatory T cells (Tregs) help control the development and maintenance of protective immunity and can lead to aberrant immune responses to some pathogens. Several lines of evidence suggest that Tregs are induced by exposure to superantigens (SAgs) in vitro or in vivo. In this study, bovine peripheral blood mononuclear cells (PBMC) were exposed in vitro to a relatively low dose (5 ng/ml) of staphy ...
DOI:
10.1128/IAI.01358-06

22. Protease-Activated Receptor 2 Mediates Human Beta-Defensin 2 and CC Chemokine Ligand 20 mRNA Expression in Response to Proteases Secreted by Porphyromonas gingivalis

Author:
Dommisch, Henrik; Chung, Whasun O.; Rohani, Maryam G.; Williams, David; Rangarajan, Minnie; Curtis, Mike A.; Dale, Beverly A.
Source:
Infection and immunity 2007 v.75 no.9 pp. 4326-4333
ISSN:
0019-9567
Subject:
immune response,   etc   ; Porphyromonas gingivalis; antimicrobial peptides; cell free system; chemokines; epithelial cells; gene expression; gene expression regulation; genes; humans; innate immunity; macrophages; messenger RNA; pathogens; proteinase inhibitors; proteinases; receptors;     Show all 17 Subjects
Abstract:
... The oral pathogen Porphyromonas gingivalis secretes proteases such as Arg-gingipain B (RgpB) that activate protease-activated receptors (PARs). Human beta-defensins (hBDs) and the macrophage inflammatory protein 3α/CC chemokine ligand 20 (CCL20) produced by epithelial cells are antimicrobial peptides that provide cytokine function and play an important role in innate immunity. The aim of the prese ...
DOI:
10.1128/IAI.00455-07
PubMed:
17591792
PubMed Central:
PMC1951157

23. Multiple Cytokine and Acute-Phase Protein Gene Transcription in West Greenland Sledge Dogs (Canis familiaris) Dietary Exposed to Organic Environmental Pollutants

Author:
Sonne, C.; Fonfara, S.; Dietz, R.; Kirkegaard, M.; Letcher, R. J.; Shahmiri, S.; Andersen, S.; Møller, P.
Source:
Archives of environmental contamination and toxicology 2007 v.53 no.1 pp. 110-118
ISSN:
0090-4341
Subject:
immune response,   etc   ; DDT (pesticide); EDTA (chelating agent); Inuit; acute phase proteins; biomarkers; blood; blubber; dogs; fatty acid-binding proteins; gene expression; genes; heat shock proteins; immune system; liver; messenger RNA; persistent organic pollutants; polychlorinated biphenyls; predators; skidders; whales; Arctic region; Greenland;     Show all 23 Subjects
Abstract:
... Exposure levels of persistent organic pollutants, such as polychlorinated biphenyls and DDTs, are high in Arctic apex predators and Inuit peoples and are suspected to have negative impacts on their immune systems. We conducted a controlled generational study on liver tissue and ethylenediaminetetraacetic acid blood cytokine and acute-phase protein (APP) mRNA expressions using reverse transcription ...
DOI:
10.1007/s00244-006-0135-y
PubMed:
17396211

24. Downregulation of the goat β-defensin-2 gene by IL-4 in caprine intestinal epithelial cells infected with Eimeria spp.

Author:
Ibarra-Velarde, F.; Alcala-Canto, Y.
Source:
Parasitology research 2007 v.101 no.3 pp. 613-618
ISSN:
0932-0113
Subject:
immune response,   etc   ; Eimeria; antimicrobial peptides; cattle; coccidiosis; enzyme-linked immunosorbent assay; epithelial cells; gene expression regulation; genes; goats; interferon-gamma; interleukin-4; messenger RNA; mononuclear leukocytes; parasites; pathogenesis; reverse transcriptase polymerase chain reaction; secretion;     Show all 18 Subjects
Abstract:
... Defensins are antimicrobial peptides that are produced by leukocytes and epithelial cells. Recent advances indicate that these peptides play an important role in innate immune responses. Nonetheless, the role of defensins in caprine eimeriosis remains unknown. Therefore, this study investigated the expression of a goat β-defensin, named GBD-2 in caprine intestinal epithelial cells (CIEC) stimulate ...
DOI:
10.1007/s00436-007-0523-x

25. Salmonid microarrays identify intestinal genes that reliably monitor P deficiency in rainbow trout aquaculture

Author:
Kirchner, S.; McDaniel, N.K.; Sugiura, S.H.; Soteropoulos, P.; Tian, B.; Fletcher, J.W.; Ferraris, R.P.
Source:
Animal genetics 2007 v.38 no.4 pp. 319-331
ISSN:
0268-9146
Subject:
immune response,   etc   ; Oncorhynchus mykiss; Salmo salar; aquaculture; biochemical pathways; biomarkers; blood serum; carbonate dehydratase; diet; farmers; farms; financial economics; gene expression; gene expression regulation; genes; intestines; messenger RNA; microarray technology; phosphates; phosphorus; pollution; proteolysis; trout;     Show all 23 Subjects
Abstract:
... Nutrient-responsive genes can identify important metabolic pathways and evaluate optimal dietary levels. Using a 16K Salmo salar microarray, we identified in rainbow trout (Oncorhynchus mykiss) 21 potential phosphorus (P)-responsive genes, mainly involved in immune response, proteolysis or transport, whose expression levels changed in the intestine after 5 days of feeding a low-P (LP) diet. Diet-i ...
DOI:
10.1111/j.1365-2052.2007.01615.x
PubMed:
17596124

26. Electroacupuncture alters the expression of genes associated with lipid metabolism and immune reaction in liver of hypercholesterolemia mice

Author:
Kang, Yaoxia; Li, Min; Yan, Wenqi; Li, Xingjie; Kang, Jinmei; Zhang, Yizheng
Source:
Biotechnology letters 2007 v.29 no.12 pp. 1817-1824
ISSN:
0141-5492
Subject:
immune response,   etc   ; acupuncture; cholesteremic effect; cholesterol; gene expression; gene expression regulation; genes; hypercholesterolemia; inflammation; lipid metabolism; liver; messenger RNA; mice; suppression subtractive hybridization;     Show all 14 Subjects
Abstract:
... Acupuncture or electroacupuncture (EA) is effective in treating various metabolism disorders. Previously we found that EA at the acupoint, Fenglong (ST40), had the cholesterol-lowering effect and regulated genes expression in liver of hypercholesterolemia mice (M Li and YZ Zhang, Int J Mol Med 2007, 19: 617-629). To explain gene expression associated with EA, suppression subtractive hybridization ...
DOI:
10.1007/s10529-007-9482-7
PubMed:
17704895

27. Analysis of Porcine Transcriptional Response to Salmonella enterica serovar Choleraesuis suggests novel targets of NFkappaB are activated in the Mesenteric Lymph Node

Author:
Wang, Yanfang; Couture, Oliver P.; Qu, Long; Uthe, Jolita J.; Bearson, Shawn M.D.; Kuhar, Daniel; Lunney, Joan K.; Nettleton, Dan; Dekkers, Jack C.M.; Tuggle, Christopher K.
Source:
BMC genomics 2008 v.9 no.437
ISSN:
1471-2164
Subject:
immune response,   etc   ; swine; salmonellosis; Salmonella enterica subsp. enterica serovar Choleraesuis; host-pathogen relationships; transcription (genetics); gene expression; gene expression regulation; transcription factors; lymph nodes; messenger RNA; microarray technology; genes; inflammation; antigen presentation; dendritic cells; cytokines; chemokines; promoter regions;     Show all 19 Subjects
Abstract:
... Background: Specific knowledge of the molecular pathways controlling host-pathogen interactions can increase our understanding of immune response biology as well as provide targets for drug development and genetic improvement of disease resistance. Toward this end, we have characterized the porcine transcriptional response to Salmonella enterica serovar Choleraesuis (S. Choleraesuis), a Salmonella ...
Handle:
10113/40341
DOI:
10.1186/1471-2164-9-437
PubMed:
18811943
PubMed Central:
PMC2570369

28. Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle: Focus on Mammalian Embryogenomics

Author:
Mitko, Katrin; Ulbrich, Susanne E.; Wenigerkind, Hendrik; Sinowatz, Fred; Blum, Helmut; Wolf, Eckhard; Bauersachs, Stefan
Source:
Reproduction 2008 v.135 no.2 pp. 225-240
ISSN:
1470-1626
Subject:
immune response,   etc   ; Simmental; angiogenesis; biochemical pathways; cell adhesion; cell growth; collagen; complementary DNA; diestrus; endometrium; estrogens; estrus; extracellular matrix; gene expression; gene interaction; genes; heifers; messenger RNA; metestrus; microarray technology; morphogenesis; oxytocin; progesterone; tight junctions; transcriptome;     Show all 25 Subjects
Abstract:
... During the oestrous cycle, the bovine endometrium exhibits characteristic morphological and functional changes, which are mainly induced by progesterone (P₄), oestrogens and oxytocin. We studied the response of the endometrium to this changing hormonal environment at the transcriptome level using a custom-made cDNA microarray. Endometrium samples were recovered from Simmental heifers on days 0 (oe ...
DOI:
10.1530/REP-07-0415

29. The Failure of Immune Response Evasion by Linear Plasmid 28-1-Deficient Borrelia burgdorferi Is Attributable to Persistent Expression of an Outer Surface Protein

Author:
Embers, Monica E.; Alvarez, Xavier; Ooms, Tara; Philipp, Mario T.
Source:
Infection and immunity 2008 v.76 no.9 pp. 3984-3991
ISSN:
0019-9567
Subject:
immune response,   etc   ; Borrelia burgdorferi; Lyme disease; antigens; gene expression regulation; genes; immune evasion; lipoproteins; messenger RNA; mice; outer membrane proteins; pathogenicity; plasmids; rats; severe combined immunodeficiency; surface proteins;     Show all 16 Subjects
Abstract:
... Infectivity and persistence by Borrelia burgdorferi, the etiologic agent of Lyme disease, rely stringently on regulatory events. Among these is the downregulation of lipoprotein antigen expression, exemplified by outer surface protein C (OspC), at the advent of specific immunity in the mammalian host. B. burgdorferi spirochetes that lack the linear plasmid 28-1 (lp28-1) succumb to the host's immun ...
DOI:
10.1128/IAI.00387-08
PubMed:
18625742
PubMed Central:
PMC2519429

30. Cloning of canine Toll-like receptor 7 gene and its expression in dog tissues

Author:
Okui, Y.; Kano, R.; Maruyama, H.; Hasegawa, A.
Source:
Veterinary immunology and immunopathology 2008 v.121 no.1-2 pp. 156-160
ISSN:
0165-2427
Subject:
immune response,   etc   ; interspecific variation; messenger RNA; complementary DNA; physiological regulation; normal values; dogs; reverse transcriptase polymerase chain reaction; amino acid sequences; gene expression; animal organs; genes; immune system; molecular cloning; receptors; nucleotide sequences; animal tissues; interferons;     Show all 18 Subjects
Abstract:
... Toll-like receptor 7 (TLR7) is activated by single strand RNA and imidazoquinoline compounds, and induces interferon production. In this study, canine TLR7 cDNA was cloned and sequenced. The full-length cDNA of canine TLR7 gene was 3419 bp, encoding 1032 amino acids. The similarities of canine TLR7 with human and mouse TLR7 were 84 and 80% at the nucleotide sequence level, and 86 and 79% at amino ...
DOI:
10.1016/j.vetimm.2007.08.012

31. Functional aspects of animal microRNAs

Author:
Williams, A. E.
Source:
Cellular and molecular life sciences 2008 v.65 no.4 pp. 545-562
ISSN:
1420-682X
Subject:
immune response,   etc   ; cell differentiation; cholesterol metabolism; genes; heart diseases; hematopoiesis; insulin secretion; mammals; messenger RNA; microRNA; muscle development; neurogenesis; ribonucleoproteins; skeletal muscle; stem cells; translation (genetics);     Show all 16 Subjects
Abstract:
... MicroRNAs (miRNAs) are a recently discovered family of small regulatory molecules that function by modulating protein production. There are approximately 500 known mammalian miRNA genes, and each miRNA may regulate hundreds of different protein-coding genes. Mature miRNAs bind to target mRNAs in a protein complex known as the miRNA-induced silencing complex (miRISC), sometimes referred to as the m ...
DOI:
10.1007/s00018-007-7355-9
PubMed:
17965831

32. Analysis of gene transcript profiling and immunobiology in Shetland sheepdogs with dermatomyositis

Author:
Wahl, Jacquelyn M.; Clark, Leigh Anne; Skalli, Omar; Ambrus, Andy; Rees, Christine A.; Mansell, Joanne L.; Murphy, Keith E.
Source:
Veterinary dermatology 2008 v.19 no.2 pp. 52-58
ISSN:
0959-4493
Subject:
immune response,   etc   ; messenger RNA; Shetland Sheepdog; immunohistochemistry; dog diseases; dogs; skin diseases; gene expression; genes; myositis; autoimmune diseases; fluorescent antibody technique; autoantibodies;     Show all 13 Subjects
Abstract:
... Dermatomyositis (DM) is a canine and human inflammatory disease of the skin and muscle that is thought to be autoimmune in nature. In dogs, DM occurs most often in the rough collie and Shetland sheepdog. Characteristic skin lesions typically develop on the face, ears, tail, and distal extremities. The severity of lesions varies and is thought to increase with stressful stimuli. Previous studies in ...
DOI:
10.1111/j.1365-3164.2008.00655.x
PubMed:
18336421

33. Gene (mRNA) expression in canine atopic dermatitis: microarray analysis

Author:
Merryman-Simpson, Annemarie E.; Wood, Shona H.; Fretwell, Neale; Jones, Paul G.; McLaren, William M.; McEwan, Neil A.; Clements, Dylan N.; Carter, Stuart D.; Ollier, William E.; Nuttall, Tim
Source:
Veterinary dermatology 2008 v.19 no.2 pp. 59-66
ISSN:
0959-4493
Subject:
immune response,   etc   ; dogs; dog diseases; atopic dermatitis; pathogenesis; animal genetics; gene expression; genetic variation; genetic polymorphism; epidemiological studies; genomics; microarray technology; genes; messenger RNA; statistical analysis; computer software; skin lesions;     Show all 17 Subjects
Abstract:
... Genes potentially involved in the pathology of canine atopic dermatitis (AD) were identified using gene expression microarrays. Total RNA extracted from skin biopsies was hybridized to an Agilent Technologies custom-designed 22K canine array. The arrays were analysed using Genedata Analyst software. Data were corrected for multiple hypothesis testing and tested for significance using the National ...
DOI:
10.1111/j.1365-3164.2008.00653.x
PubMed:
18336422

34. The malaria vector mosquito Anopheles gambiae expresses a suite of larval-specific defensin genes

Author:
Meredith, J.M.; Hurd, H.; Lehane, M.J.; Eggleston, P.
Source:
Insect molecular biology 2008 v.17 no.2 pp. 103-112
ISSN:
0962-1075
Subject:
immune response,   etc   ; messenger RNA; complementary DNA; cell culture; antimicrobial peptides; insect vectors; response elements; genes; Anopheles gambiae; larvae; malaria;     Show all 11 Subjects
Abstract:
... cDNAs of Anopheles gambiae Defensin 2 (AgDef2), Defensin 3 (AgDef3) and Defensin 4 (AgDef4), identified in the genome sequence, have been characterized and their expression profiles investigated. In contrast to both typical defensins and insect antimicrobial peptides generally, the newly identified defensins were not upregulated with acute-phase kinetics following immune challenge in insects or ce ...
DOI:
10.1111/j.1365-2583.2008.00786.x
PubMed:
18353100
PubMed Central:
PMC2459261

35. Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells

Author:
Shinohara, Mari L.; Kim, Hye-Jung; Kim, June-Ho; Garcia, Virgilio A.; Cantor, Harvey
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.20 pp. 7235-7239
ISSN:
0027-8424
Subject:
immune response,   etc   ; bioactive properties; bone formation; cytokines; dendritic cells; genes; messenger RNA; osteopontin; phenotype; secretion; secretory granules; signal peptide; translation (genetics);     Show all 13 Subjects
Abstract:
... Osteopontin (Opn) contributes to diverse biological processes that include immune responses, vascularization, and bone formation. Until recently, studies describing the activities of Opn have focused on the cytokine-like properties of the secreted protein. Here, we show that alternative translation of a single Opn mRNA species generates a secreted and intracellular isoform. Utilization of a 5' can ...
DOI:
10.1073/pnas.0802301105
PubMed:
18480255
PubMed Central:
PMC2438233

36. Salmonella Enteritidis-Induced Alteration of Inflammatory CXCL Chemokine Messenger-RNA Expression and Histologic Changes in the Ceca of Infected Chicks

Author:
Cheeseman, J.H.; Levy, N.A.; Kaiser, P.; Lillehoj, H.S.; Lamont, S.J.
Source:
Avian diseases 2008 v.52 no.2 pp. 229
ISSN:
0005-2086
Subject:
immune response,   etc   ; chicks; Salmonella enteritidis; salmonellosis; inflammation; protein synthesis; interleukins; interferons; gene expression; genes; cytokines; cecum; reverse transcriptase polymerase chain reaction; messenger RNA; macrophages; apoptosis; chemokines;     Show all 17 Subjects
Abstract:
... To understand better the events in early avian host immune responses to Salmonella Enteritidis (SE), we examined messenger-RNA (mRNA) expression for eight genes: CXCLi1[K60], CXCLi2 [IL-8/CAF], interferon (IFN)-, interleukin (IL) -1, IL-6, IL-12, IL-12, and gallinacin (Gal)-2 in the ceca of young chicks 1 wk postinoculation with SE. Cecum tissue sections were stained and evaluated for the presence ...
Handle:
10113/19080
DOI:
10.1637/8156-102307-Reg.1
PubMed:
18646451

37. Toll-like receptor gene expression in cecum and spleen of advanced intercross line chicks infected with Salmonella enterica serovar Enteritidis

Author:
Abasht, B.; Kaiser, M.G.; Lamont, S.J.
Source:
Veterinary immunology and immunopathology 2008 v.123 no.3-4 pp. 314-323
ISSN:
0165-2427
Subject:
immune response,   etc   ; chickens; messenger RNA; females; microbial load; Salmonella Enteritidis; cecum; poultry diseases; gene expression regulation; males; gender differences; gene expression; spleen; genes; disease resistance; salmonellosis; receptors; line differences; chicks;     Show all 19 Subjects
Abstract:
... Understanding the changes in host gene expression that occur with bacterial infection will help to elucidate the basis of molecular genetic control of disease resistance. The effect of infecting chicks with Salmonella enterica serovar Enteritidis on the RNA expression level of Toll-like receptor (TLR) genes, and the correlation between TLR RNA expression level and bacterial burden in the cecum and ...
DOI:
10.1016/j.vetimm.2008.02.010
PubMed:
18394716

38. The expression profile of TLR9 mRNA and CpG ODNs immunostimulatory actions in the teleost gilthead seabream points to a major role of lymphocytes

Author:
Cuesta, A.; Esteban, M. A.; Meseguer, J.
Source:
Cellular and molecular life sciences 2008 v.65 no.13 pp. 2091-2104
ISSN:
1420-682X
Subject:
immune response,   etc   ; DNA; Sparus aurata; Toll-like receptor 9; aquaculture; cytotoxicity; fish; genes; humans; lymphocytes; messenger RNA; mice; oligodeoxyribonucleotides; reactive oxygen species; tissues;     Show all 15 Subjects
Abstract:
... The potential effects of synthetic unmethylated oligodeoxynucleotides (ODN) containing CpG motifs, mimicking bacterial DNA, has never been evaluated on the immune response in the teleost fish gilthead seabream (Sparus aurata), the most important fish species in Mediterranean aquaculture. First, binding and competition studies have demonstrated that binding is saturated and promiscuous, suggesting ...
DOI:
10.1007/s00018-008-8146-7
PubMed:
18535781

39. Reduced nitric oxide production and iNOS mRNA expression in IFN-γ-stimulated chicken macrophages transfected with iNOS siRNAs

Author:
Cheeseman, Jennifer H.; Lillehoj, Hyun S.; Lamont, Susan J.
Source:
Veterinary immunology and immunopathology 2008 v.125 no.3-4 pp. 375
ISSN:
0165-2427
Subject:
immune response,   etc   ; chickens; immune system; biochemical pathways; genes; gene expression; messenger RNA; nitric oxide; enzymes; nitric oxide synthase; interferons; signal transduction; in vitro studies; cell lines; macrophages; transfection; RNA interference; gene expression regulation; inhibitors; enzyme inhibition; interferon-gamma;     Show all 21 Subjects
Abstract:
... Utilizing RNA interference technology with siRNA in the HD11 macrophage cell line, we determined how the inhibition or knock-down of the iNOS (inducible nitric oxide synthase) gene affected IFN-γ-induced macrophage production of nitric oxide (NO) and mRNA expression of genes involved in this biological pathway in the chicken. Chicken macrophages produce NO when stimulated with recombinant chicken ...
Handle:
10113/21458
DOI:
10.1016/j.vetimm.2008.05.015

40. Expression patterns in soybean resistant to Phakopsora pachyrhizi reveal the importance of peroxidases and lipoxygenases

Author:
Choi, J. J.; Alkharouf, N. W.; Schneider, K. T.; Matthews, B. F.; Frederick, R. D.
Source:
Functional & integrative genomics 2008 v.8 no.4 pp. 341
ISSN:
1438-793X
Subject:
immune response,   etc   ; Phakopsora pachyrhizi; plant pathogenic fungi; disease resistance; soybeans; rust diseases; genes; complementary DNA; messenger RNA; microarray technology; expressed sequence tags; lipoxygenase; peroxidases; molecular cloning;     Show all 14 Subjects
Abstract:
... Soybean rust caused by Phakopsora pachyrhizi Sydow is a devastating foliar disease that has spread to most soybean growing regions throughout the world, including the USA. Four independent rust resistance genes, Rpp1-Rpp4, have been identified in soybean that recognize specific isolates of P. pachyrhizi. A suppressive subtraction hybridization (SSH) complementary DNA (cDNA) library was constructed ...
Handle:
10113/19370
DOI:
10.1007/s10142-008-0080-0

41. Transcription of Inflammatory Genes in the Lung after Infection with Community-Associated Methicillin-Resistant Staphylococcus aureus: a Role for Panton-Valentine Leukocidin?

Author:
Montgomery, Christopher P.; Daum, Robert S.
Source:
Infection and immunity 2009 v.77 no.5 pp. 2159-2167
ISSN:
0019-9567
Subject:
immune response,   etc   ; Staphylococcus aureus; messenger RNA; inflammation; pneumonia; reverse transcriptase polymerase chain reaction; transcription (genetics); virulence; genes; animal models; lungs; mutants; chemokines; mortality; inoculum; rats;     Show all 16 Subjects
Abstract:
... Necrotizing pneumonia caused by community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates is increasingly common and frequently severe. The early inflammatory response in the lung after CA-MRSA infection remains largely undefined. Additionally, many workers have hypothesized that the Panton-Valentine leukocidin (PVL) is a key virulence determinant in CA-MRSA necrotizing p ...
DOI:
10.1128/IAI.00021-09
PubMed:
19237525
PubMed Central:
PMC2681736

42. CTdatabase: a knowledge-base of high-throughput and curated data on cancer-testis antigens

Author:
Almeida, Luiz Gonzaga; Sakabe, Noboru J.; deOliveira, Alice R.; Silva, Maria Cristina C.; Mundstein, Alex S.; Cohen, Tzeela; Chen, Yao-Tseng; Chua, Ramon; Gurung, Sita; Gnjatic, Sacha; Jungbluth, Achim A.; Caballero, Otávia L.; Bairoch, Amos; Kiesler, Eva; White, Sarah L.; Simpson, Andrew J.G.; Old, Lloyd J.; Camargo, Anamaria A.; Vasconcelos, Ana Tereza R.
Source:
Nucleic acids research 2009 v.37 no.suppl_1 pp. D816
ISSN:
0305-1048
Subject:
immune response,   etc   ; T-lymphocytes; antigens; bioinformatics; clinical trials; databases; gametogenesis; gene expression; genes; histology; humans; melanoma; messenger RNA; patients; protein synthesis; proteins; tissues; vaccines;     Show all 18 Subjects
Abstract:
... The potency of the immune response has still to be harnessed effectively to combat human cancers. However, the discovery of T-cell targets in melanomas and other tumors has raised the possibility that cancer vaccines can be used to induce a therapeutically effective immune response against cancer. The targets, cancer-testis (CT) antigens, are immunogenic proteins preferentially expressed in normal ...

43. Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene

Author:
Cwiklinski, Krystyna; Meskill, Diana; Robinson, Mark W.; Pozio, Eduardo; Appleton, Judith A.; Connolly, Bernadette
Source:
Veterinary parasitology 2009 v.159 no.3-4 pp. 268-271
ISSN:
0304-4017
Subject:
immune response,   etc   ; interspecific variation; Trichinella pseudospiralis; messenger RNA; serine proteinases; sequence alignment; hosts; host-parasite relationships; skeletal muscle; Trichinella spiralis; amino acid sequences; animal parasitic nematodes; genes; nucleotide sequences; nematode larvae; immune system; genetic variation; protein secretion; isozymes; life cycle (organisms);     Show all 20 Subjects
Abstract:
... Nematode parasites of the genus Trichinella are intracellular and distinct life cycle stages invade intestinal epithelial and skeletal muscle cells. Within the genus, Trichinella spiralis and Trichinella pseudospiralis exhibit species-specific differences with respect to host-parasite complex formation and host immune modulation. Parasite excretory-secretory (ES) proteins play important roles at t ...
DOI:
10.1016/j.vetpar.2008.10.036
PubMed:
19054614
PubMed Central:
PMC2673510

44. Genomic analysis of mucosal immunobiology in the porcine small intestine

Author:
Dvorak, Cheryl M.T.; Hirsch, Geoffrey N.; Murtaugh, Michael P.
Source:
Veterinary immunology and immunopathology 2009 v.128 no.1-3 pp. 119-125
ISSN:
0165-2427
Subject:
immune response,   etc   ; Peyer's patches; adjuvants; antigen-antibody complex; antigens; cDNA libraries; cholera toxin; complementary DNA; cultured cells; databases; gene expression; gene expression regulation; genes; genomics; hosts; infectious diseases; messenger RNA; mucosal immunity; pathogens; proteins; small intestine; swine; vaccines;     Show all 23 Subjects
Abstract:
... The vast majority of infectious diseases of animals and humans occur at mucosal surfaces, especially in the intestinal tract. However, vaccines to stimulate durable immunity to intestinal pathogens often do not produce effective protection. A better understanding of the molecules and molecular mechanisms that mediate effective immune induction at mucosal surfaces may facilitate the development of ...
DOI:
10.1016/j.vetimm.2008.10.304
PubMed:
19027174

45. Molecular analysis of tammar (Macropus eugenii) mammary epithelial cells stimulated with lipopolysaccharide and lipoteichoic acid

Author:
Daly, Kerry A.; Mailer, Sonia L.; Digby, Matthew R.; Lefévre, Christophe; Thomson, Peter; Deane, Elizabeth; Nicholas, Kevin R.; Williamson, Peter
Source:
Veterinary immunology and immunopathology 2009 v.129 no.1-2 pp. 36-48
ISSN:
0165-2427
Subject:
immune response,   etc   ; lipopolysaccharides; cell growth; epithelial cells; genes; maternal milk; protective effect; leukocytes; gene expression; transcriptome; mammary glands; apoptosis; transcriptomics; messenger RNA; physiological response; cell proliferation; antigen presentation; microarray technology; inflammation; genomics; Macropus eugenii;     Show all 21 Subjects
Abstract:
... The immunological function of the metatherian mammary gland plays a crucial part in neonatal survival of the marsupial young. Marsupial pouch young do not develop adult like immune responses until just prior to leaving the pouch. The immune components of the maternal milk secretions are important during this vulnerable early post-partum period. In addition, infection of the mammary gland has not b ...
DOI:
10.1016/j.vetimm.2008.12.001
PubMed:
19157568

46. Both treated and untreated tumors are eliminated by short hairpin RNA-based induction of target-specific immune responses

Author:
Gu, Wenyi; Cochrane, Melanie; Leggatt, Graham R.; Payne, Elizabeth; Choyce, Allison; Zhou, Fang; Tindle, Robert; McMillan, Nigel A.J.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.20 pp. 8314-8319
ISSN:
0027-8424
Subject:
immune response,   etc   ; RNA interference; T-lymphocytes; adaptive immunity; animal models; cytotoxicity; epitopes; genes; messenger RNA; mice; neoplasm cells; neoplasms; proteins; small interfering RNA; therapeutics; translation (genetics); viral antigens; viruses;     Show all 18 Subjects
Abstract:
... RNA interference (RNAi) for cancer treatment relies on the ability to directly kill cancer cells via down-regulation of target genes, but issues of delivery and efficacy have limited clinical adoption. Furthermore, current studies using immune-deficient animal models disregard potential interactions with the adaptive immune system. It has previously been observed that certain viral antigens appear ...
DOI:
10.1073/pnas.0812085106
PubMed:
19416823
PubMed Central:
PMC2688843

47. Determination of internal control for gene expression studies in equine tissues and cell culture using quantitative RT-PCR

Author:
Zhang, Yuwen W.; Davis, Elizabeth G.; Bai, Jianfa
Source:
Veterinary immunology and immunopathology 2009 v.130 no.1-2 pp. 114-119
ISSN:
0165-2427
Subject:
immune response,   etc   ; gene expression; in vitro studies; animal tissues; cell culture; blood cells; genes; complementary DNA; messenger RNA; microarray technology; reverse transcriptase polymerase chain reaction; fluorescent labeling; biomarkers; ribosomal RNA; validity; accuracy; reference standards; statistical analysis; horses;     Show all 19 Subjects
Abstract:
... Quantitative reverse transcription polymerase chain reaction (RT-PCR) has become a basic, reliable and sensitive modern technique, in both biological research and clinical diagnosis, for investigation of gene expression and validation of cDNA microarray analysis. Accurate mRNA quantification using quantitative RT-PCR commonly requires data normalization through stable housekeeping genes (HKGs). Se ...
DOI:
10.1016/j.vetimm.2009.01.012
PubMed:
19269038

48. Expression of porcine Toll-like receptor 2, 4 and 9 gene transcripts in the presence of lipopolysaccharide and Salmonella enterica serovars Typhimurium and Choleraesuis

Author:
Burkey, T.E.; Skjolaas, K.A.; Dritz, S.S.; Minton, J.E.
Source:
Veterinary immunology and immunopathology 2009 v.130 no.1-2 pp. 96-101
ISSN:
0165-2427
Subject:
immune response,   etc   ; bacterial enteritis; messenger RNA; Salmonella choleraesuis Choleraesuis; ileum; lipopolysaccharides; swine diseases; epithelial cells; Salmonella Typhimurium; gene expression; jejunum; salmonellosis; phagocytes; intestinal mucosa; genes; receptors; swine;     Show all 17 Subjects
Abstract:
... Salmonella enterica serovar Typhimurium (ST) and Choleraesuis (SC) are among the most frequently isolated salmonellae serovars causing enteric disease in swine. Enteric disease in young pigs is of major concern in modern production systems due to the negative implications on animal health, food safety and economic return. Epithelial cells express Toll-like receptors (TLR) that recognize conserved ...
DOI:
10.1016/j.vetimm.2008.12.027

49. Cell population, viability, and some key immunomodulatory molecules in different milk somatic cell samples in dairy cows

Author:
Baumert, Amandine; Bruckmaier, Rupert M.; Wellnitz, Olga
Source:
Journal of dairy research 2009 v.76 no.3 pp. 356-364
ISSN:
0022-0299
Subject:
immune response,   etc   ; milk; somatic cell count; somatic cells; viability; dairy cows; immunomodulators; udders; lipopolysaccharides; Escherichia coli; temporal variation; messenger RNA; apoptosis; polymerase chain reaction; animal proteins; genes; protein synthesis; cultured cells; immune system; leukocyte count;     Show all 20 Subjects
Abstract:
... Immune cells in the milk are most important in combating pathogens that invade the mammary gland. This study investigated the immune competence and viability of somatic milk cells that are already resident in milk and udders free of infection. Cells were studied in freshly removed milk to simulate conditions in the udder. Effects of incubation, cell preparation, and immunological stimulation with ...
DOI:
10.1017/S0022029909004129
PubMed:
19445829

50. Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

Author:
Imamura, Saiki; da Silva Vaz, Itabajara Jr.; Konnai, Satoru; Yamada, Shinji; Nakajima, Chie; Onuma, Misao; Ohashi, Kazuhiko
Source:
Experimental & applied acarology 2009 v.48 no.4 pp. 345-358
ISSN:
0168-8162
Subject:
immune response,   etc   ; Haemaphysalis longicornis; genes; messenger RNA; metalloproteinases; mortality; rabbits; reverse transcriptase polymerase chain reaction; salivary glands; signal peptide; ticks; vaccination;     Show all 12 Subjects
Abstract:
... We report the cloning, expression and characterization of an Haemaphysalis longicornis metalloprotease (named HLMP1). The gene encodes a predicted 550 aminoacid protein with similarity to metalloproteases of the reprolysin family. The protein sequence contains a signal sequence, the zinc-binding motif (HEXXHXXGXXH) common to metalloproteases and a cysteine-rich region. Reverse transcription-PCR ex ...
DOI:
10.1007/s10493-009-9245-3
PubMed:
19184465