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1. Pullulan biosynthesis in yeast-like fungal cells is regulated by the transcriptional activator Msn2 and cAMP-PKA signaling pathway

Author:
Yang, Guang,   et al.   ; Liu, Guang-Lei; Wang, Shu-Jun; Chi, Zhen-Ming; Chi, Zhe;     Show all 5 Authors
Source:
International journal of biological macromolecules 2020 v.157 pp. 591-603
ISSN:
0141-8130
Subject:
Aureobasidium; biosynthesis; cytoplasm; fungi; gene expression; genes; lipids; phosphorylase; pullulan; signal transduction; transactivators
Abstract:
... Pullulan is an important polysaccharide. Although its synthetic pathway in Aureobasidium melanogenum has been elucidated, the mechanism underlying its biosynthesis as regulated by signaling pathway and transcriptional regulator is still unknown. In this study, it was found that the expression of the UGP1 gene encoding UDPG-pyrophosphorylase (Ugp1) and other genes which were involved in pullulan bi ...
DOI:
10.1016/j.ijbiomac.2020.04.174

2. Glutamate related osmoregulation of guanine nucleotide-binding protein G (I) α2 from giant freshwater prawn (Macrobrachium rosenbergii) during molting and salinity stress

Author:
Yang, Guang,   et al.   ; Lu, Zhijie; Qin, Zhendong; Shen, Haiyang; Zhang, Menglan; Shi, Fei; Li, Jun; Sarath Babu, V.; Lin, Li;     Show all 9 Authors
Source:
Aquaculture 2020 v.521 pp. 735000
ISSN:
0044-8486
Subject:
G-proteins; Macrobrachium rosenbergii; WD40 repeats; brackish water; brain; cAMP-dependent protein kinase; freshwater; gene expression regulation; genes; gills; glutamic acid; heart; hemocytes; hepatopancreas; intestines; larvae; messenger RNA; molting; muscle tissues; muscles; open reading frames; osmoregulation; osmotic pressure; osmotic stress; phylogeny; salt stress; shrimp; stomach
Abstract:
... Giant freshwater prawn (Macrobrachium rosenbergii) is one of the most important cultured freshwater prawn species. Although giant freshwater prawn lives in freshwater, brackish water environment is essential for their larval stage of development. M. rosenbergii has to regulate osmotic pressure through osmoregulation, no matter it lives in either brackish water in the course of their larval stage o ...
DOI:
10.1016/j.aquaculture.2020.735000

3. Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella

Author:
Yang, Guang,   et al.   ; Fu, Shu; Liu, Zhaoxia; Chen, Jinzhi; Sun, Gengxiao; Jiang, Yingxia; Li, Miaowen; Xiong, Lei; Chen, Shaoping; Zhou, Yuqing; Asad, Muhammad;     Show all 11 Authors
Source:
Pest management science 2020 v.76 no.5 pp. 1761-1771
ISSN:
1526-498X
Subject:
Arabidopsis thaliana; CD29 antigen; Plutella xylostella; RNA interference; arginine kinase; bioassays; crops; double-stranded RNA; genes; insect control; insect pests; mortality; plant protection; pupae; pupation; transcription (genetics); transgenic plants
Abstract:
... BACKGROUND: Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double‐stranded RNA (dsRNA) targeting P. xylostella genes of arginine ...
DOI:
10.1002/ps.5701

4. Alternative primers are required for pullulan biosynthesis in Aureobasidium melanogenum P16

Author:
Yang, Guang,   et al.   ; Chen, Tie-Jun; Liu, Guang-Lei; Chen, Lu; Hu, Zhong; Chi, Zhen-Ming; Chi, Zhe;     Show all 7 Authors
Source:
International journal of biological macromolecules 2020 v.147 pp. 10-17
ISSN:
0141-8130
Subject:
Aureobasidium; amylases; biosynthesis; catalytic activity; ceramides; genes; glucosylceramides; glycogen; glycogen (starch) synthase; glycosides; industry; mutants; pullulan; starch; sterols; yeasts
Abstract:
... Although pullulan has many uses in industry, the detailed mechanisms of its biosynthesis still require clarification. In this study, it was found that a short α-1,4-glucosyl chain (pullulan primer) synthesized by the glycogenins Glg1 and Glg2 for initiation of glycogen biosynthesis was also needed for pullulan synthesis. The primers were also synthesized on sterol glycosides and glucosylceramides ...
DOI:
10.1016/j.ijbiomac.2020.01.049

5. The anti‐fungal β‐sitosterol targets the yeast oxysterol‐binding protein Osh4

Author:
Yang, Guang‐Fu,   et al.   ; Moosavi, Behrooz; Liu, Shuting; Wang, Nan‐Nan; Zhu, Xiao‐Lei;     Show all 5 Authors
Source:
Pest management science 2020 v.76 no.2 pp. 704-711
ISSN:
1526-498X
Subject:
Saccharomyces cerevisiae; aerobic conditions; antifungal properties; beta-sitosterol; cholesterol; genes; metabolites; molecular models; mutation; pest management; plant pathogenic fungi; prediction; proteins; toxicity; yeasts
Abstract:
... BACKGROUND: β‐Sitosterol is a plant metabolite with a broad range of anti‐fungal activity, however, this compound is not toxic against a few fungal species. The target of β‐sitosterol and the nature of its selective toxicity are not yet clear. Using a yeast model system and taking advantage of molecular biology and computational approaches, we identify the target and explain why β‐sitosterol is no ...
DOI:
10.1002/ps.5568

6. Distinct evolution of toll-like receptor signaling pathway genes in cetaceans

Author:
Yang, Guang,   et al.   ; Tian, Ran; Seim, Inge; Zhang, Zepeng; Yang, Ying; Ren, Wenhua; Xu, Shixia;     Show all 7 Authors
Source:
Genes & genomics 2019 v.41 no.12 pp. 1417-1430
ISSN:
1976-9571
Subject:
Escherichia coli; Gram-negative bacteria; Phocoenidae; Toll-like receptor 4; amino acids; cattle; coevolution; dolphins; freshwater; genes; human cell lines; humans; immune system; immunity; kidneys; lipopolysaccharides; pathogens; physicochemical properties; signal transduction; whales
Abstract:
... BACKGROUND: The relatively rapid spread and diversity of marine pathogens posed an initial and ongoing challenge for cetaceans (whales, dolphins, and porpoises), descendants of terrestrial mammals that transitioned from land to sea approximately 56 million years ago. Toll-like receptors (TLRs) play important roles in regulating immunity against pathogen infections by detecting specific molecular p ...
DOI:
10.1007/s13258-019-00861-3

7. Genome sequencing of Aureobasidium pullulans P25 and overexpression of a glucose oxidase gene for hyper-production of Ca2+-gluconic acid

Author:
Yang, Guang,   et al.   ; Zhao, Shou-Feng; Jiang, Hong; Chi, Zhe; Liu, Guang-Lei; Chi, Zhen-Ming; Chen, Tie-Jun; Hu, Zhong;     Show all 8 Authors
Source:
Antonie van Leeuwenhoek 2019 v.112 no.5 pp. 669-678
ISSN:
0003-6072
Subject:
Aureobasidium pullulans; binding sites; biosynthesis; food industry; gene overexpression; genes; gluconic acid; glucose oxidase; pharmaceutical industry; sequence analysis; signal peptide; yeasts
Abstract:
... Gluconic acid (GA) has many applications such as in the food and pharmaceutical industry. Aureobasidium pullulans P25 strain is able to produce high levels of Ca²⁺-GA. The genome length, GC content and the gene number of this yeast were found to be 30.97 Mb, 50.28% and 10,922, respectively. The pathways for gluconic acid biosynthesis were annotated. Glucose oxidase (Gox) sequences from different s ...
DOI:
10.1007/s10482-018-1197-3

8. Cloning of flavonoid 3′,5′-hydroxylase and 3′-hydroxylase homologs from black currant (Ribes nigrum) and their differential expression at various fruit maturation stages

Author:
Yang, Guang,   et al.   ; Li, Xing-guo; Yang, Tian-tian; Yu, Ze-yuan; Huo, Jun-wei; Dong, Qin; Duan, Ya-dong;     Show all 7 Authors
Source:
Journal of forestry research 2019 v.30 no.2 pp. 463-470
ISSN:
1007-662X
Subject:
Ribes nigrum; anthocyanins; biosynthesis; black currants; complementary DNA; enzymes; fruits; gene expression regulation; genes; phylogeny
Abstract:
... Flavonoid 3′-hydroxylase (F3′H) and 3′,5′-hydroxylase (F3′5′H) generate precursor molecules for the synthesis of cyanidin-based anthocyanins (red) and delphinidin-based anthocyanins (blue to purple) in Ribes nigrum L. (black currant). In this study, full-length 1780 and 1650-bp cDNA homologs of RnF3′5′H1 and RnF3′H1 from black currant were identified and cloned using a homologous cloning technique ...
DOI:
10.1007/s11676-017-0560-y

9. Characteristics of archaea and bacteria in rice rhizosphere along a mercury gradient

Author:
Yang, Guang,   et al.   ; Ma, Ming; Du, Hongxia; Sun, Tao; An, Siwei; Wang, Dingyong;     Show all 6 Authors
Source:
The Science of the total environment 2019 v.650 pp. 1640-1651
ISSN:
0048-9697
Subject:
Methanosarcina; Thiobacillus; Xanthomonas; bacteria; genes; mercury; methylation; methylmercury compounds; paddies; paddy soils; rhizosphere; ribosomal RNA; rice; species diversity; China
Abstract:
... Several strains of archaea have the ability to methylate or resist mercury (Hg), and the paddy field is regarded to be conducive to Hg methylation. However, our knowledge of Hg-methylating or Hg-resistant archaea in paddy soils is very limited so far. Therefore, the distribution of archaea and bacteria in the rhizosphere (RS) and bulk soil (BS) of the rice growing in Xiushan Hg-mining area of sout ...
DOI:
10.1016/j.scitotenv.2018.07.175

10. Genome-wide identification and functional analysis of the splicing component SYF2/NTC31/p29 across different plant species

Author:
Yang, Guang-Fu,   et al.   ; Tian, Yuan; Chen, Mo-Xian; Yang, Jing-Fang; Achala, H. H. K.; Gao, Bei; Hao, Ge-Fei; Dian, Zhi-Yong; Hu, Qi-Juan; Zhang, Di; Zhang, Jianhua; Liu, Ying-Gao;     Show all 12 Authors
Source:
Planta 2019 v.249 no.2 pp. 583-600
ISSN:
0032-0935
Subject:
Arabidopsis; DNA repair; Liliopsida; alternative splicing; bioinformatics; carcinogenesis; cell cycle; databases; functional diversity; genes; humans; models; phylogeny; plant development; promoter regions; protein isoforms; proteins; rice; stress response; transcription (genetics); yeasts
Abstract:
... MAIN CONCLUSION: This study systematically identifies plant SYF2/NTC31/p29 genes from 62 plant species by a combinatory bioinformatics approach, revealing the importance of this gene family in phylogenetics, duplication, transcriptional, and post-transcriptional regulation. Alternative splicing is a post-transcriptional regulatory mechanism, which is critical for plant development and stress respo ...
DOI:
10.1007/s00425-018-3026-3

11. Variation in Photosynthetic Traits and Correlation with Growth in Teak (<i>Tectona grandis</i> Linn.) Clones

Author:
Yang, Guang,   et al.   ; Huang, Guihua; Liang, Kunnan; Zhou, Zaizhi; Muralidharan, Enarth Maviton;     Show all 5 Authors
Source:
Forests 2019 v.10 no.1
ISSN:
1999-4907
Subject:
Tectona grandis; breeding; carbon dioxide; chlorophyll; clones; field experimentation; gas exchange; genes; genetic variation; germplasm; growth traits; heritability; photosynthesis; plant height; provenance; resource management; seedlings; wood volume
Abstract:
... In order to interpret the patterns of genetic variation of photosynthesis and the relationships with growth traits within gene resources of teak (Tectona grandis Linn.), gas exchange, and chlorophyll fluorescence parameters, growth traits of plants in nursery and field trials were measured for 20 teak clones originated from different countries. The results show that there was abundant genetic vari ...
DOI:
10.3390/f10010044

12. Amphiphilic core-shell nanoparticles: Synthesis, biophysical properties, and applications

Author:
Yang, Guang,   et al.   ; Panday, Raju; Poudel, Abhishek Jung; Li, Xiaohong; Adhikari, Manjila; Ullah, Muhammad Wajid;     Show all 6 Authors
Source:
Colloids and surfaces 2018 v.172 pp. 68-81
ISSN:
0927-7765
Subject:
biocompatibility; biodegradability; colloids; drugs; gases; genes; heavy metals; immobilized enzymes; molecular weight; nanocarriers; nanoparticles; polymers; remediation; toxic substances; toxicity
Abstract:
... Recent advancements in amphiphilic core shell (ACS) nanoparticles have resulted in the development of multifunctional nanocarriers for broad spectrum applications. The ACS nanoparticles are synthesized between organic/organic and organic/inorganic molecules or entities making them highly versatile delivery vehicles. The shape, size, and biological features such as biocompatibility and biodegradabi ...
DOI:
10.1016/j.colsurfb.2018.08.019

13. Identification and expression of complement component C8α, C8β and C8γ gene in half-smooth tongue sole (Cynoglossus semilaevis) and C8α recombinant protein antibacterial activity analysis

Author:
Yang, Guang,   et al.   ; Xiu, Yunji; Chen, Yadong; Bai, Li; Sha, Zhenxia;     Show all 5 Authors
Source:
Fish & shellfish immunology 2018 v.72 pp. 658-669
ISSN:
1050-4648
Subject:
Komagataella pastoris; plasmids; genes; blood; Takifugu rubripes; open reading frames; recombinant proteins; Cynoglossus semilaevis; Vibrio anguillarum; amino acids; intestines; spleen; Vibrio parahaemolyticus; Edwardsiella tarda; tissues; complementary DNA; yeasts; growth retardation; immune response; kidneys; antibacterial properties; Siniperca chuatsi; complement; liver; Oryzias latipes
Abstract:
... Complement component C8, which mediates membrane attack complex formation and bacterial lysis, plays important roles in the complement system. The cDNA sequences of the C8α, C8β and C8γ genes were cloned from half-smooth tongue sole (Cynoglossus semilaevis). Full-length cDNA of CsC8α (C8α of C. semilaevis), CsC8β and CsC8γ was 1990, 2219 and 886 bp, respectively, which contained open reading frame ...
DOI:
10.1016/j.fsi.2017.11.017

14. Thymoquinone reduces kidney damage in apolipoprotein E-deficient mice fed a high-cholesterol diet

Author:
Yang, Guang,   et al.   ; Pei, Zuowei; Zhu, Liyue; Liu, Yingshu; Li, Nan; Liu, Hongyang;     Show all 6 Authors
Source:
RSC advances 2017 v.7 no.83 pp. 53002-53009
ISSN:
2046-2069
Subject:
Nigella sativa; active ingredients; anti-inflammatory activity; cholesterol; cytokines; genes; high fat diet; hypercholesterolemia; kidney diseases; kidneys; low density lipoprotein; macrophages; males; mice; oxidation; protective effect; protein synthesis; quinones; rats; risk factors; seeds; staining; therapeutics; tissues
Abstract:
... Hypercholesterolemia is a well-established risk factor for kidney injury that can lead to chronic kidney disease (CKD). Thymoquinone (TQ) is one of the most active ingredients in Nigella Sativa seeds. It has a variety of beneficial properties including anti-oxidative and anti-inflammatory activities. TQ exerts positive effects on DOX-induced nephropathy and ischaemia-reperfusion induced kidney inj ...
DOI:
10.1039/c7ra07040c

15. Functional characterization of Pol III U6 promoters for gene knockdown and knockout in Plutella xylostella

Author:
Yang, Guang,   et al.   ; Huang, Yuping; Wang, Yajun; Zeng, Baosheng; Liu, Zhaoxia; Xu, Xuejiao; Meng, Qian; Huang, Yongping; Vasseur, Liette; Gurr, Geoff M.; You, Minsheng;     Show all 11 Authors
Source:
Insect biochemistry and molecular biology 2017 v.89 pp. 71-78
ISSN:
0965-1748
Subject:
DNA-directed RNA polymerase; Plutella xylostella; TATA box; gene editing; genes; loci; messenger RNA; mutagenesis; pests; phenotype; plasmids; small interfering RNA; small nuclear RNA
Abstract:
... RNA polymerase type III (Pol-III) promoters such as U6 are commonly used to express small RNAs, including short hairpin RNAs (shRNAs) and single guide RNAs (sgRNAs). Functional U6 promoters are widely used in CRISPR systems, and their characterization can facilitate genome editing of non-model organisms. In the present study, six U6 small nuclear RNA (snRNA) promoters containing two conserved elem ...
DOI:
10.1016/j.ibmb.2017.08.009

16. QTL effects and epistatic interaction for flowering time and branch number in a soybean mapping population of Japanese×Chinese cultivars

Author:
YANG, Guang,   et al.   ; ZHAI, Hong; WU, Hong-yan; ZHANG, Xing-zheng; LÜ, Shi-xiang; WANG, Ya-ying; LI, Yu-qiu; HU, Bo; WANG, Lu; WEN, Zi-xiang; WANG, De-chun; WANG, Shao-dong; Kyuya, Harada; XIA, Zheng-jun; XIE, Fu-ti;     Show all 15 Authors
Source:
Journal of integrative agriculture 2017 v.16 no.9 pp. 1900-1912
ISSN:
2095-3119
Subject:
agronomic traits; branching; chromosome mapping; crop yield; cultivars; epistasis; flowering date; genes; genetic background; genetic factors; loci; quantitative trait loci; single nucleotide polymorphism; soybeans
Abstract:
... Flowering time and branching type are important agronomic traits related to the adaptability and yield of soybean. Molecular bases for major flowering time or maturity loci, E1 to E4, have been identified. However, more flowering time genes in cultivars with different genetic backgrounds are needed to be mapped and cloned for a better understanding of flowering time regulation in soybean. In this ...
DOI:
10.1016/S2095-3119(16)61539-3

17. Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme

Author:
Yang, Guang-Yu,   et al.   ; Zhuang, Yu; Chen, Xiaohui; Liu, Qian; Zhang, Xueli; Deng, Zixin; Feng, Yan;     Show all 7 Authors
Source:
Metabolic engineering 2017 v.42 pp. 25-32
ISSN:
1096-7176
Subject:
Panax; Saccharomyces cerevisiae; antineoplastic agents; batch fermentation; bioreactors; biosynthesis; catalytic activity; genes; medicinal plants; metabolic engineering; mutants; yeasts
Abstract:
... Ginsenoside Rh2 is a potential anticancer drug isolated from medicinal plant ginseng. Fermentative production of ginsenoside Rh2 in yeast has recently been investigated as an alternative strategy compared to extraction from plants. However, the titer was quite low due to low catalytic capability of the key ginseng glycosyltransferase in microorganisms. Herein, we have demonstrated high-level produ ...
DOI:
10.1016/j.ymben.2017.04.009

18. Trace element concentrations, risks and their correlation with metallothionein genes polymorphism: A case study of narrow-ridged finless porpoises (Neophocaena asiaeorientalis) in the East China Sea

Author:
Yang, Guang,   et al.   ; Liu, Jun; Chen, Bingyao; Jefferson, Thomas Allen; Wang, Hui;     Show all 5 Authors
Source:
The Science of the total environment 2017 v.575 pp. 628-638
ISSN:
0048-9697
Subject:
Neophocaena; arsenic; body length; cadmium; case studies; chromium; copper; genes; kidneys; lead; liver; manganese; mercury; metallothionein; muscle tissues; nickel; pollution; risk; risk assessment; zinc; East China Sea
Abstract:
... The concentration of trace elements (TEs) and their risk to narrow-ridged finless porpoises (Neophocaena asiaeorientalis) are still unclear. The present study determined the concentration of typical TEs in liver, kidney, and muscle tissues from porpoises in the East China Sea, assessed potential health risk of TEs to porpoises, and explored the relationship between TE concentration and metallothio ...
DOI:
10.1016/j.scitotenv.2016.09.062

19. Deletion of the transcriptional regulator GntR down regulated the expression of Genes Related to Virulence and Conferred Protection against Wild-Type Brucella Challenge in BALB/c Mice

Author:
Yang, Guang-Li,   et al.   ; Li, Zhi-Qiang; Zhang, Jin-Liang; Xi, Li; Wang, Shu-Li; Zhang, Xiao-Gen; Zhang, Jun-Bo; Zhang, Hui;     Show all 8 Authors
Source:
Molecular Immunology 2017
ISSN:
0161-5890
Subject:
Brucella; brucellosis; gene expression; gene expression regulation; genes; immune response; immunoglobulin G; interferon-gamma; interleukin-4; live vaccines; macrophages; mice; mutants; public health; secretion; transcription factors; virulence
Abstract:
... Brucellosis, which is caused by Brucella spp., is a zoonotic infectious disease that can cause great hazard to public health and safety. The virulence of Brucella is essential for survive and multiply in host macrophages. GntR is a transcriptional regulator in Brucella that is required for virulence in macrophages and mice, and involved in resistance to stress responses. To determine the expressio ...
DOI:
10.1016/j.molimm.2017.10.011

20. Activation of CO2-reducing methanogens in oil reservoir after addition of nutrient

Author:
Yang, Guang-Chao,   et al.   ; Zhou, Lei; Mbadinga, Serge Maurice; You, Jing; Yang, Hua-Zhen; Liu, Jin-Feng; Yang, Shi-Zhong; Gu, Ji-Dong; Mu, Bo-Zhong;     Show all 9 Authors
Source:
Journal of bioscience and bioengineering 2016 v.122 no.6 pp. 740-747
ISSN:
1389-1723
Subject:
Firmicutes; Methanobacteriales; Methanomicrobiales; Methanosaetaceae; biomarkers; community structure; energy recovery; genes; methane production; methanogens; microbial communities; multidimensional scaling; oil fields; oils; petroleum; quantitative analysis; quantitative polymerase chain reaction
Abstract:
... Nutrient addition as part of microbial enhanced oil recovery (MEOR) operations have important implications for more energy recovery from oil reservoirs, but very little is known about the in situ response of microorganisms after intervention. An analysis of two genes as biomarkers, mcrA encoding the key enzyme in methanogenesis and fthfs encoding the key enzyme in acetogenesis, was conducted durin ...
DOI:
10.1016/j.jbiosc.2016.06.011

21. Microbial communities responsible for fixation of CO2 revealed by using mcrA, cbbM, cbbL, fthfs, fefe-hydrogenase genes as molecular biomarkers in petroleum reservoirs of different temperatures

Author:
Yang, Guang-Chao,   et al.   ; Liu, Jin-Feng; Mbadinga, Serge Maurice; Sun, Xiao-Bo; Yang, Shi-Zhong; Gu, Ji-Dong; Mu, Bo-Zhong;     Show all 7 Authors
Source:
International biodeterioration & biodegradation 2016 v.114 pp. 164-175
ISSN:
0964-8305
Subject:
Actinobacteria; Firmicutes; Methanobacteriales; Methanosarcinales; Proteobacteria; bacteria; biomarkers; biotransformation; carbon dioxide; carbon dioxide fixation; carbon sequestration; energy; functional diversity; genes; methane; methanogens; microbial communities; oil fields; petroleum; recycling; surveys; temperature
Abstract:
... Sequestration of CO2 in oil reservoir is one of the feasible options for mitigating atmospheric CO2 building up. The in situ bioconversion of sequestrated CO2 to methane by microorganisms inhabiting oil reservoirs is feasible. To evaluate the potential of in situ microbial fixation and conversion of CO2 into CH4 in oil reservoirs, a comprehensive molecular survey was performed to reveal microbial ...
DOI:
10.1016/j.ibiod.2016.06.019

22. Association of polymorphisms in the MyD88, IRAK4 and TRAF6 genes and susceptibility to type 2 diabetes mellitus and diabetic nephropathy in a southern Han Chinese population

Author:
Yang, Guang,   et al.   ; Guo, Congcong; Zhang, Liju; Nie, Lihong; Zhang, Na; Xiao, Di; Ye, Xingguang; Ou, Meiling; Liu, Yang; Zhang, Baohuan; Wang, Man; Lin, Hansheng; Jing, Chunxia;     Show all 13 Authors
Source:
Molecular and Cellular Endocrinology 2016 v.429 pp. 114-119
ISSN:
0303-7207
Subject:
Chinese people; diabetic nephropathy; genes; haplotypes; inflammation; models; noninsulin-dependent diabetes mellitus; patients; risk; signal transduction; single nucleotide polymorphism; transcription factor NF-kappa B
Abstract:
... Type 2 diabetes mellitus (T2DM) has been linked to a state of low-grade inflammation resulting from abnormalities in the innate immune pathway. MyD88 is an essential adaptor protein for TLR signaling, which is involved in activating NF-κB through IRAK4 and TRAF6. To investigate the effects of the MyD88, IRAK4 and TRAF6 polymorphisms in the susceptibility of T2DM and diabetic vascular complications ...
DOI:
10.1016/j.mce.2016.04.003

23. Mitochondrial genomes of Heterakis gallinae and Heterakis beramporia support that they belong to the infraorder Ascaridomorpha

Author:
Yang, Guang-You,   et al.   ; Wang, Bao-Jian; Gu, Xiao-Bin; Wang, Tao; Lai, Wei-Min; Zhong, Zhi-Jun; Liu, Guo-Hua;     Show all 7 Authors
Source:
Infection, genetics, and evolution 2016 v.40 pp. 228-235
ISSN:
1567-1348
Subject:
Ascaridia; Heterakis; chickens; financial economics; genes; mitochondrial genome; molecular epidemiology; nucleotide sequences; parasites; phylogeny; population genetics; ribosomal RNA; transfer RNA
Abstract:
... Heterakis gallinae and Heterakis beramporia are the most prevalent nematode infecting native chicken breed, causing major economic losses. In the present study, the complete mitochondrial genomes (mt) of H. gallinae and H. beramporia were amplified by long-PCR and then sequenced. The complete mt genomes of H. gallinae and H. beramporia were 13,973bp and 14,012bp in size, respectively. Both mt geno ...
DOI:
10.1016/j.meegid.2016.03.012

24. Taurine protects against As2O3-induced autophagy in pancreas of rat offsprings through Nrf2/Trx pathway

Author:
Yang, Guang,   et al.   ; Bai, Jie; Yao, Xiaofeng; Jiang, Liping; Qiu, Tianming; Liu, Shuang; Qi, Baoxu; Zheng, Yue; Kong, Yuan; Chen, Min; Liu, Xiaofang; Sun, Xiance;     Show all 12 Authors
Source:
Biochimie 2016 v.123 pp. 1-6
ISSN:
0300-9084
Subject:
arsenic; autophagy; genes; noninsulin-dependent diabetes mellitus; nutrition; pancreas; pathogenesis; rats; reactive oxygen species; risk factors; taurine
Abstract:
... Arsenic was increasingly to blame as a risk factor for type 2 diabetes mellitus. In our previous study, we had found iAs stimulated autophagic flux and caused autophagic cell death through ROS pathway in INS-1 cells. Since NF-E2-related factor 2 (Nrf2) and the thioredoxin (Trx) system was a crucial line of defense against ROS, we investigated whether Nrf2/Trx pathway contributed to As2O3-stimulate ...
DOI:
10.1016/j.biochi.2016.01.002

25. Investigation of the ASR family in foxtail millet and the role of ASR1 in drought/oxidative stress tolerance

Author:
Yang, Guang-Xiao,   et al.   ; Feng, Zhi-Juan; Xu, Zhao-Shi; Sun, Jiutong; Li, Lian-Cheng; Chen, Ming; He, Guang-Yuan; Ma, You-Zhi;     Show all 8 Authors
Source:
Plant cell reports 2016 v.35 no.1 pp. 115-128
ISSN:
0721-7714
Subject:
drought tolerance; phylogeny; drought; genes; oxidative stress; bioenergy; homeostasis; Setaria italica; tissues; transcription factors; plant development; germination; gene overexpression; water content; root growth; abiotic stress; enzyme activity; tobacco; stress tolerance; crop models; survival rate; stress response; ripening; abscisic acid; chlorophyll; crops
Abstract:
... KEY MESSAGE : Six foxtail millet ASR genes were regulated by various stress-related signals. Overexpression of ASR1 increased drought and oxidative tolerance by controlling ROS homeostasis and regulating oxidation-related genes in tobacco plants. Abscisic acid stress ripening (ASR) proteins with ABA/WDS domains constituted a class of plant-specific transcription factors, playing important roles in ...
DOI:
10.1007/s00299-015-1873-y

26. Distribution and phylogenetic analysis of Culex flavivirus in mosquitoes in China

Author:
Yang, Guang,   et al.   ; Liang, Wenkai; He, Xiaoxia; Liu, Guifang; Zhang, Shaobai; Fu, Shihong; Wang, Min; Chen, Weixin; He, Ying; Tao, Xiaoyan; Jiang, Hongyue; Lin, Xiaojuan; Gao, Xiaoyan; Hu, Weijun; Liu, Yan; Feng, Lei; Cao, Yuxi; Jing, Chunxia; Liang, Guodong; Wang, Huanyu;     Show all 20 Authors
Source:
Archives of virology 2015 v.160 no.9 pp. 2259-2268
ISSN:
0304-8608
Subject:
Anopheles sinensis; Culex; Flavivirus; RNA; biogeography; genes; genotype; geographical distribution; phylogeny; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; viruses; Africa; Central America; China; Japan; South America; United States
Abstract:
... Culex flavivirus (CxFV) is an insect-specific virus of the genus Flavivirus. CxFV strains have been isolated from Cx. pipiens, Cx. quinquefasciatus, and other Cx. species in Asia, Africa, North America, Central America and South America. CxFV was isolated for the first time in China in 2006. As this is a novel flavivirus, we explored the distribution and genetic characteristics of Culex flavivirus ...
DOI:
10.1007/s00705-015-2492-1

27. Functional characterization of CCR in birch (Betula platyphylla × Betula pendula) through overexpression and suppression analysis

Author:
Yang, Guang,   et al.   ; Zhang, Wenbo; Wei, Rui; Chen, Su; Jiang, Jing; Li, Huiyu; Huang, Haijiao; Wang, Shuo; Wei, Hairong; Liu, Guifeng;     Show all 10 Authors
Source:
Physiologia plantarum 2015 v.154 no.2 pp. 283-296
ISSN:
0031-9317
Subject:
apical meristems; tree growth; genetically modified organisms; biosynthesis; gene overexpression; Betula pendula subsp. mandshurica; wood; phylogeny; trees; genes; xylem; biomass; lignin
Abstract:
... We cloned a Cinnamoyl‐CoA Reductase gene (BpCCR1) from an apical meristem and first internode of Betula platyphylla and characterized its functions in lignin biosynthesis, wood formation and tree growth through transgenic approaches. We generated overexpression and suppression transgenic lines and analyzed them in comparison with the wild‐type in terms of lignin content, anatomical characteristics ...
DOI:
10.1111/ppl.12306

28. Current status and trends of wheat genetic transformation studies in China

Author:
YANG, Guang-xiao,   et al.   ; HE, Yi; WANG, Qiong; ZENG, Jian; SUN, Tao; HE, Guang-yuan;     Show all 6 Authors
Source:
Journal of integrative agriculture 2015 v.14 no.3 pp. 438-452
ISSN:
2095-3119
Subject:
biosafety; breeding; genes; genetic engineering; genetic transformation; genetically modified organisms; genomics; methodology; wheat; China
Abstract:
... More than 20 years have passed since the first report on successful genetic transformation of wheat. With the establishment and improvement of transformation platform, great progresses have been made on wheat genetic transformation both on its fundamental and applied studies in China, especially driven by the National Major Project for Transgenic Organism Breeding, China, initiated in 2008. In thi ...
DOI:
10.1016/S2095-3119(14)60934-5

29. Over-expression of the MxIRT1 gene increases iron and zinc content in rice seeds

Author:
Yang, Guang,   et al.   ; Tan, Song; Han, Rui; Li, Peng; Li, Shuang; Zhang, Peng; Wang, Wei-Bing; Zhao, Wei-Zhong; Yin, Li-Ping;     Show all 9 Authors
Source:
Transgenic research 2015 v.24 no.1 pp. 109-122
ISSN:
0962-8819
Subject:
Malus; absorption; apples; biofortification; cadmium; gene overexpression; genes; genotype; iron; phenotype; protoplasts; rice; seeds; transgenic plants; trees; zinc
Abstract:
... Iron and zinc are essential in plant and human nutrition. Iron deficiency has been one of the causes of human mortality, especially in developing countries with high rice consumption. MxIRT1 is a ferrous transporter that has been screened from an iron-efficient genotype of the apple tree, Malus xiaojinensis Cheng et Jiang. In order to produce Fe-biofortified rice with MxIRT1 to solve the Fe-defici ...
DOI:
10.1007/s11248-014-9822-z
PubMed:
25099285

30. RNA-Seq analysis of differentially expressed genes in rice under varied nitrogen supplies

Author:
Yang, Guang-zhe,   et al.   ; Yang, Shun-ying; Hao, Dong-li; Song, Zhi-zhong; Wang, Li; Su, Yan-hua;     Show all 6 Authors
Source:
Gene 2015 v.555 pp. 305-317
ISSN:
0378-1119
Subject:
Oryza sativa; RNA; aldehyde dehydrogenase; amino acids; gene expression; gene expression regulation; genes; nitrogen; nitrogen metabolism; paddy soils; rice; roots; sequence analysis; shoots; surveys
Abstract:
... Ammonium is the main inorganic nitrogen source in paddy soil. Rice (Oryza sativa), an ammonium-preferring and -tolerant grain crop, is a valuable resource for researching ammonium-uptake mechanism and understanding the molecular networks that the plant copes with ammonium variation. To generate a broad survey of early responses affected by varied ammonium supplies in rice, RNA samples were prepare ...
DOI:
10.1016/j.gene.2014.11.021

31. BpGH3.5, an early auxin-response gene, regulates root elongation in Betula platyphylla × Betula pendula

Author:
Yang, Guang,   et al.   ; Chen, Su; Wang, Shuo; Liu, Guifeng; Li, Huiyu; Huang, Haijiao; Jiang, Jing;     Show all 7 Authors
Source:
Plant cell, tissue, and organ culture 2015 v.120 no.1 pp. 239-250
ISSN:
0167-6857
Subject:
Arabidopsis thaliana; apical meristems; genetically modified organisms; metabolism; phenotype; gene overexpression; Betula pendula subsp. mandshurica; hypocotyls; organ culture; reverse transcriptase polymerase chain reaction; cytokinins; genes; root growth; woody plants; roots; indole acetic acid
Abstract:
... The GH3 family is an important class of early auxin-response genes involved in the development of the hypocotyls and roots in Arabidopsis thaliana, but the role of this gene family in woody plants is poorly understood. In this study, we cloned a GH3-like gene from Betula platyphylla × Betula pendula (birch) named BpGH3.5 and produced transgenic birch lines that overexpressed either a sense or anti ...
DOI:
10.1007/s11240-014-0599-9

32. Y chromosome haplotype diversity of domestic sheep (Ovis aries) in northern Eurasia

Author:
Yang, Guang‐Li,   et al.   ; Zhang, Min; Peng, Wei‐Feng; Lv, Feng‐Hua; Liu, Ming‐Jun; Li, Wen‐Rong; Liu, Yong‐Gang; Li, Jin‐Quan; Wang, Feng; Shen, Zhi‐Qiang; Zhao, Sheng‐Guo; Hehua, EEr; Marzanov, Nurbiy; Murawski, Maziek; Kantanen, Juha; Li, Meng‐Hua;     Show all 16 Authors
Source:
Animal genetics 2014 v.45 no.6 pp. 903-907
ISSN:
0268-9146
Subject:
Y chromosome; chromosome translocation; domestication; gene frequency; genes; genetic variation; haplotypes; microsatellite repeats; rams; sheep breeds; single nucleotide polymorphism; China; Eurasia
Abstract:
... Variation in two SNPs and one microsatellite on the Y chromosome was analyzed in a total of 663 rams representing 59 breeds from a large geographic range in northern Eurasia. SNPA‐oY1 showed the highest allele frequency (91.55%) across the breeds, whereas SNPG‐oY1 was present in only 56 samples. Combined genotypes established seven haplotypes (H4, H5, H6, H7, H8, H12 and H19). H6 dominated in nort ...
DOI:
10.1111/age.12214
PubMed:
25204383

33. Depiction of Grapevine Phenology by Gene Expression Information and a Test of its Workability in Guiding Fertilization

Author:
Yang, Guang,   et al.   ; Wang, Chen; Han, Jian; Shangguan, Lingfei; Kayesh, Emrul; Zhang, Yanyi; Leng, Xiangpeng; Fang, Jinggui;     Show all 8 Authors
Source:
Plant molecular biology reporter 2014 v.32 no.5 pp. 1070-1084
ISSN:
0735-9640
Subject:
Vitis; flowers; gene expression; genes; phenology; plant growth; precision agriculture; prediction
Abstract:
... The development of precision agriculture calls for the emergence of new approaches to more accurately depict plant phenology. Gene expression data can predict and indicate plant growth state and phenological events accurately at the molecular level, and thus could be developed as a novel means of describing crop phenophase. Here, we analyzed the expression profiles of nine genes involved in grapev ...
DOI:
10.1007/s11105-014-0711-7

34. Isolation and Characterization of an Endosperm-Specific Promoter from Wheat (Triticum aestivum L.)

Author:
Yang, Guang-Xiao,   et al.   ; Song, Fei; Cui, Cui-Ju; Chen, Ling; Sun, Yang-Liu; Wang, Fei-Fei; Hussain, Javeed; Li, Yin; Wang, Chen; Wang, Cheng; Chen, Ming-Jie; Wang, Yue-Sheng; He, Guang-Yuan;     Show all 13 Authors
Source:
Zeitschrift für Naturforschung 2014 v.67 no.11-12 pp. 611-619
ISSN:
1865-7125
Subject:
sequence analysis; quantitative analysis; staining; transgenic plants; prolamins; nucleotides; wheat; gene expression; endosperm; genes; Triticum aestivum; new family; promoter regions; beta-glucuronidase; tobacco
Abstract:
... Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements of the ALP type-B gene were ascertained through sequence analysis which revealed that this fragment contains the TATA and C ...
DOI:
10.1515/znc-2012-11-1212

35. TLR2-ICAM1-Gadd45α Axis Mediates the Epigenetic Effect of Selenium on DNA Methylation and Gene Expression in Keshan Disease

Author:
Yang, Guang,   et al.   ; Zhu, Yanhe; Dong, Xin; Duan, Zongming; Niu, Xiaolin; Wei, Jin;     Show all 6 Authors
Source:
Biological trace element research 2014 v.159 no.1-3 pp. 69-80
ISSN:
0163-4984
Subject:
DNA; DNA methylation; animal models; cardiomyopathy; cell culture; epigenetics; etiology; gene expression; gene expression regulation; genes; genomic islands; inflammation; messenger RNA; myocardium; patients; precipitin tests; promoter regions; rats; reverse transcriptase polymerase chain reaction; selenium
Abstract:
... Keshan disease (KD) is a fatal dilated cardiomyopathy with unknown etiology, and selenium deficiency is considered the main cause of KD. Several observations implicate a role for altered DNA methylation in selenium deficiency-related diseases. The aim of the present study was to investigate the epigenetic effects of selenium (Se) on DNA methylation and gene expression in Keshan disease. Using meth ...
DOI:
10.1007/s12011-014-9985-8
PubMed:
24811888

36. Comprehensive profiling of Epstein-Barr virus-encoded miRNA species associated with specific latency types in tumor cells

Author:
Yang, Guang-Da,   et al.   ; Yang, Hong-Jie; Huang, Tie-Jun; Yang, Chang-Fu; Peng, Li-Xia; Liu, Ran-Yi; Chu, Qiao-Qiao; Huang, Jia-Ling; Liu, Na; Huang, Hong-Bing; Zhu, Zhen-Yu; Qian, Chao-Nan; Huang, Bi-Jun;     Show all 13 Authors
Source:
Virology journal 2013 v.10 no.1 pp. 314
ISSN:
1743-422X
Subject:
Human gammaherpesvirus 4; biomarkers; carcinogenesis; carcinoma; carrier state; cell lines; cluster analysis; epithelium; gene expression regulation; genes; microRNA; microarray technology; neoplasm cells; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; transcriptome
Abstract:
... BACKGROUND: Epstein-Barr virus (EBV) is an etiological cause of many human lymphocytic and epithelial malignancies. EBV expresses different genes that are associated with three latency types. To date, as many as 44 EBV-encoded miRNA species have been found, but their comprehensive profiles in the three types of latent infection that are associated with various types of tumors are not well document ...
DOI:
10.1186/1743-422X-10-314

37. A sensitive and specific PCR assay for the detection of Baylisascaris schroederi eggs in giant panda feces

Author:
Yang, Guang-You,   et al.   ; Wang, Ning; Li, De-Sheng; Zhou, Xuan; Xie, Yue; Liang, Yi-Nan; Wang, Cheng-Dong; Yu, Hua; Chen, Shi-Jie; Yan, Yu-Bo; Gu, Xiao-Bin; Wang, Shu-Xian; Peng, Xue-Rong;     Show all 13 Authors
Source:
Parasitology international 2013 v.62 no.5 pp. 435-436
ISSN:
1383-5769
Subject:
Ailuropoda melanoleuca; Ancylostoma caninum; Baylisascaris; DNA; cytochrome-c oxidase; disease surveillance; eggs; environmental monitoring; feces; genes; hosts; parasites; parasitology; polymerase chain reaction
Abstract:
... Baylisascaris schroederi is one of the most common intestinal nematodes in giant pandas. It can cause severe baylisascariasis which is highly infectious in its natural hosts. A rapid and reliable diagnosis of parasite infections is crucial to protect giant pandas, as well as for environmental monitoring and disease surveillance. Here, we established a specific PCR assay for B. schroederi detection ...
DOI:
10.1016/j.parint.2013.05.004

38. Genetic variability of Echinococcus granulosus from the Tibetan plateau inferred by mitochondrial DNA sequences

Author:
Yang, Guang-You,   et al.   ; Yan, Ning; Nie, Hua-Ming; Jiang, Zhong-Rong; Yang, Ai-Guo; Deng, Shi-Jin; Guo, Li; Yu, Hua; Yan, Yu-Bao; Tsering, Dawa; Kong, Wei-Shu; Wang, Ning; Wang, Jia-Hai; Xie, Yue; Fu, Yan; Yang, De-Ying; Wang, Shu-Xian; Gu, Xiao-Bin; Peng, Xue-Rong;     Show all 19 Authors
Source:
Veterinary parasitology 2013 v.196 no.1-2 pp. 179-183
ISSN:
0304-4017
Subject:
Echinococcus granulosus; genes; genetic variation; haplotypes; hosts; humans; nucleotide sequences; patients; plateaus; population structure; China
Abstract:
... To analyse genetic variability and population structure, 84 isolates of Echinococcus granulosus (Cestoda: Taeniidae) collected from various host species at different sites of the Tibetan plateau in China were sequenced for the whole mitochondrial nad1 (894bp) and atp6 (513bp) genes. The vast majority were classified as G1 genotype (n=82), and two samples from human patients in Sichuan province wer ...
DOI:
10.1016/j.vetpar.2013.02.010
PubMed:
23523013

39. Cloning and characterization of the fatty acid-binding protein gene from the protoscolex of Taenia multiceps

Author:
Yang, Guang-you,   et al.   ; Nie, Hua-ming; Xie, Yue; Fu, Yan; Yang, Ying-dong; Gu, Xiao-bin; Wang, Shu-xian; Peng, Xi; Lai, Wei-ming; Peng, Xue-rong;     Show all 10 Authors
Source:
Parasitology research 2013 v.112 no.5 pp. 1833-1839
ISSN:
0932-0113
Subject:
parasites; Western blotting; genes; Taenia multiceps; ruminants; gel electrophoresis; immunohistochemistry; recombinant proteins; fatty acid-binding proteins; sodium; uterus; molecular weight; Escherichia coli; humans; immune response; protoscoleces; eggs; integument; zoonoses; vaccines; central nervous system diseases; reverse transcriptase polymerase chain reaction
Abstract:
... Taenia multiceps (Cestoda: Taeniidae), a worldwide cestode parasite, is emerging as an important helminthic zoonosis due to serious or fatal central nervous system disease commonly known as coenurosis in domestic and wild ruminants including humans. Herein, a fatty acid-binding protein (FABP) gene was identified from transcriptomic data in T. multiceps. This gene, which contains a complete coding ...
DOI:
10.1007/s00436-013-3328-0
PubMed:
23474657

40. LDL coating pVEGF/polyethylenimine complex enhances vascular endothelial growth factor expression

Author:
Yang, Guang,   et al.   ; Li, Jian; Feng, Min; Liang, Hailong; Zhang, Jun; Huang, Danhong; Deng, Siyun; Shen, Yuan;     Show all 8 Authors
Source:
Biotechnology and bioprocess engineering 2012 v.17 no.6 pp. 1182-1189
ISSN:
1226-8372
Subject:
cell viability; coatings; cytotoxicity; genes; low density lipoprotein; protein synthesis; transfection; vascular endothelial growth factors
Abstract:
... The major limitations to non-viral gene delivery are relatively low efficiency and cytotoxicity, which need to be addressed in the design of new vectors. In this study, negatively charged low density lipoproteins (LDL) were coated onto positively charged pVEGF/PEI complexes to form pVEGF/PEI/LDL terplexes by a two-step procedure. The biocompatible LDL was introduced to reduce the cytotoxicity of t ...
DOI:
10.1007/s12257-012-0032-3

41. Overexpression of soybean GmCBL1 enhances abiotic stress tolerance and promotes hypocotyl elongation in Arabidopsis

Author:
Yang, Guang-Xiao,   et al.   ; Li, Zhi-Yong; Xu, Zhao-Shi; He, Guang-Yuan; Chen, Ming; Li, Lian-Cheng; Ma, You-Zhi;     Show all 7 Authors
Source:
Biochemical and biophysical research communications 2012 v.427 no.4 pp. 731-736
ISSN:
0006-291X
Subject:
Arabidopsis; abiotic stress; drought; gene expression; genes; gibberellins; hypocotyls; plant hormones; plant stress; proteins; roots; seedlings; soybeans; stress tolerance; transgenic plants
Abstract:
... Although extensive studies and remarkable progress have been made with Arabidopsis calcineurin B-like proteins (CBLs), knowledge of their functions in other plant species is still limited. Here we isolated gene GmCBL1 from soybean, a homolog of AtCBL1 in Arabidopsis. GmCBL1 was differentially induced by multiple abiotic stress and plant hormones, and its transcripts were abundant in seedlings and ...
DOI:
10.1016/j.bbrc.2012.09.128

42. A mutation in Arabidopsis BSK5 encoding a brassinosteroid-signaling kinase protein affects responses to salinity and abscisic acid

Author:
Yang, Guang-Xiao,   et al.   ; Li, Zhi-Yong; Xu, Zhao-Shi; He, Guang-Yuan; Chen, Ming; Li, Lian-Cheng; Ma, You-Zhi;     Show all 7 Authors
Source:
Biochemical and biophysical research communications 2012 v.426 no.4 pp. 522-527
ISSN:
0006-291X
Subject:
Arabidopsis; abiotic stress; abscisic acid; brassinosteroids; drought; genes; mutants; mutation; physiological response; protein kinases; proteins; salinity; salt tolerance; signal transduction; tissues
Abstract:
... As the most recently characterized group of plant hormones, brassinosteroids (BR) are involved in a number of physiological responses. Although many key components of the BR signaling pathway have been isolated and characterized, there is little information on detailed characterization of brassinosteroid-signaling kinase (BSK) proteins. In this study, Arabidopsis BSK5 was isolated and functionally ...
DOI:
10.1016/j.bbrc.2012.08.118

43. Arginine Residues in the C-terminal and their Relationship with the Analgesic Activity of the Toxin from the Chinese Scorpion Buthus martensii Karsch (BmK AGP-SYPU1)

Author:
Yang, Guang-Zhao,   et al.   ; Wang, Yu; Song, Yong-Bo; Cui, Yong; Zhao, Yong-Shan; Liu, Yan-Feng; Ma, Yan; Wu, Chun-Fu; Zhang, Jing-Hai;     Show all 9 Authors
Source:
Applied biochemistry and biotechnology 2012 v.168 no.2 pp. 247-255
ISSN:
0273-2289
Subject:
Buthus martensii; analgesic effect; analgesics; arginine; genes; models; mutants; peptides; polymerase chain reaction; site-directed mutagenesis; toxins
Abstract:
... In this study, we investigated the functional role of arginines in the C-terminal (65–67) of BmK AGP-SYPU1, an analgesic peptide from the Chinese scorpion Buthus martensii Karsch. Using site-directed mutagenesis, arginines at the C-terminal (65–66) were deleted or added to the C-terminal (67). The genes for three mutants of BmK AGP-SYPU1 were obtained by PCR. An analgesic activity assay was us ...
DOI:
10.1007/s12010-012-9768-7

44. Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca

Author:
Yang, Guang,   et al.   ; Zhang, Gang; Wang, Xu; Guo, Qingjuan; Li, Ying; Li, Jilun;     Show all 6 Authors
Source:
Applied biochemistry and biotechnology 2012 v.168 no.1 pp. 116-128
ISSN:
0273-2289
Subject:
Klebsiella oxytoca; NAD (coenzyme); acetates; boiling point; byproducts; carbon; enzyme activity; ethanol; fermentation; genes; glycerol; lactates; metabolites; mutants; succinic acid; water solubility
Abstract:
... Glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-Propanediol (1,3-PD) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-BD), and ethanol were produced in the oxidative branch. 2,3-BD, which has similar properties of high boiling point and water solubility with 1,3-PD, not only contests the carbon flow and NADH with 1,3-PD b ...
DOI:
10.1007/s12010-011-9363-3

45. Characterization of a myrosinase cDNA from Brassica parachinensis and its defense role against Plutella xylostella after suppression

Author:
Yang, Guang,   et al.   ; Wang, Ding‐Feng; Dong, Zhi‐Quan; Wang, Qian‐Liang; Wang‐Pruski, Gefu; You, Min‐Sheng;     Show all 6 Authors
Source:
Insect science 2012 v.19 no.4 pp. 461-471
ISSN:
1672-9609
Subject:
Brassica rapa var. parachinensis; Plutella xylostella; RNA interference; complementary DNA; double-stranded RNA; fecundity; genes; glucosinolates; hydrolysis; insect larvae; molecular cloning; pest resistance; pupae; sequence analysis; thioglucosidase; transfection; transgenic plants
Abstract:
... In Brassicaceae, myrosinase catalyzes the hydrolysis of glucosinolate and plays an important role in anti‐herbivore defense. We have cloned and characterized the full‐length complementary DNA of myrosinase gene from Brassica parachinensis that exhibits high sequence identity with myrosinase genes from other Brassica species. To investigate the role of this myrosinase in defense against the diamond ...
DOI:
10.1111/j.1744-7917.2011.01458.x

46. NR3C1 gene polymorphism for genetic susceptibility to infantile spasms in a Chinese population

Author:
Yang, Guang,   et al.   ; Zou, Li-Ping; He, Bing; Ding, Ying-Xue; Wang, Jing; Shi, Xiu-Yu; Sun, Yan-Hong; Jia, Fei-Yong;     Show all 8 Authors
Source:
Life sciences 2012 v.91 no.1-2 pp. 37-43
ISSN:
0024-3205
Subject:
corticotropin; genes; glucocorticoid receptors; haplotypes; homozygosity; models; risk; risk reduction; single nucleotide polymorphism
Abstract:
... AIMS: To test the genetic association of NR3C1 gene which encodes the glucocorticoid receptor with infantile spasms (IS). MAIN METHODS: Nine single nucleotide polymorphisms (SNPs) within the NR3C1 gene were genotyped in a sample set of 128 cases and 131 controls. Association analysis was performed on the genotyped data. KEY FINDINGS: Two SNPs, rs10482672 and rs2963155, showed nominal associations ...
DOI:
10.1016/j.lfs.2012.06.010

47. Correlation between efficacy and structure of recombinant epitope vaccines against bovine type O foot and mouth disease virus

Author:
Yang, Guang,   et al.   ; Fang, Mingli; Li, Jianli; Wang, Hua; Yang, Ming; Zhang, Yongsheng; Zhou, Lei; Wei, Hongfei; Yu, Yue; Wei, Xuefeng; Yu, Yongli; Wang, Liying; Wan, Min;     Show all 13 Authors
Source:
Biotechnology letters 2012 v.34 no.5 pp. 839-847
ISSN:
0141-5492
Subject:
Foot-and-mouth disease virus; cattle; epitopes; foot-and-mouth disease; genes; guinea pigs; mice; neutralizing antibodies; recombinant proteins; vaccines; viruses
Abstract:
... To develop recombinant epitope vaccines against foot-and-mouth disease virus (FMDV), genes coding for six recombinant proteins (rP1–rP6) consisting of different combinations of B cell and T cell epitope from VP1 capsid protein (VP1) of type O FMDV were constructed and the 3D structure of these proteins analyzed. This revealed a surface-exposed RGD sequence of B cell epitopes in all six recombinant ...
DOI:
10.1007/s10529-012-0856-0
PubMed:
22286179

48. Differential gene expression during somatic embryogenesis in the maize (Zea mays L.) inbred line H99

Author:
Yang, Guang,   et al.   ; Sun, Lifang; Wu, Ying; Su, Shengzhong; Liu, Hongkui; Li, Shipeng; Shan, Xiaohui; Yuan, Yaping;     Show all 8 Authors
Source:
Plant cell, tissue, and organ culture 2012 v.109 no.2 pp. 271-286
ISSN:
0167-6857
Subject:
RNA; Zea mays; breeding; cell division; complementary DNA; corn; cultivars; energy conversion; enzyme activity; gene expression; genes; inbred lines; kinesin; nucleotide sequences; plant propagation; protein phosphorylation; sequence analysis; signal transduction; somatic cells; somatic embryogenesis; tyrosine
Abstract:
... Somatic embryogenesis is a complex developmental process that offers great potential for plant propagation. Although many studies have shown that the generation of embryonic cells from somatic cells is accompanied by the synthesis of RNA and DNA and by elevated enzymatic activity, the mechanism of the onset of somatic embryogenesis is not well understood. cDNA-amplified fragment length polymorphis ...
DOI:
10.1007/s11240-011-0093-6

49. Phylogenomic analyses and improved resolution of Cetartiodactyla

Author:
Yang, Guang,   et al.   ; Zhou, Xuming; Xu, Shixia; Yang, Yunxia; Zhou, Kaiya;     Show all 5 Authors
Source:
Molecular phylogenetics and evolution 2011 v.61 no.2 pp. 255-264
ISSN:
1055-7903
Subject:
Carnivora; Hippopotamidae; Perissodactyla; Tursiops; autocorrelation; data collection; dolphins; ecology; genes; mitochondrial DNA; models; monophyly; nesting; nucleotide sequences; rivers
Abstract:
... The remarkable antiquity, diversity, and significance in the ecology and evolution of Cetartiodactyla have inspired numerous attempts to resolve their phylogenetic relationships. However, previous analyses based on limited samples of nuclear genes or mitochondrial DNA sequences have generated results that were either inconsistent with one another, weakly supported, or highly sensitive to analytica ...
DOI:
10.1016/j.ympev.2011.02.009
PubMed:
21315162

50. Molecular cloning, expression and characterization of the functional domain of CTLA4 from the rhesus monkey, Macaca mulatta

Author:
Yang, Guang,   et al.   ; Zhu, Shengyun; Liu, Shan; Wan, Lin; Yang, Hao; Cheng, Jingqiu; Lu, Xiaofeng;     Show all 7 Authors
Source:
Developmental and comparative immunology 2011 v.35 no.7 pp. 736-744
ISSN:
0145-305X
Subject:
asparagine; immunoglobulin G; concanavalin A; leucine; serine; antigens; T-lymphocytes; Komagataella pastoris; antibodies; cytotoxicity; humans; immunotherapy; Macaca mulatta; mice; genes; methionine; molecular cloning; immunologic diseases
Abstract:
... Cytotoxic T lymphocyte-associated antigen 4 (CTLA4) is a potent inhibitor of T cell activation. The genes encoding the membrane and soluble forms of Macaca mulatta CTLA4 (mmCTLA4) were isolated from peripheral blood mononuclear cells (PBMCs). The predicted mmCTLA4 protein is nearly identical to human CTLA4 (hCTLA4), with the exception of a serine instead of an asparagine residue at position 49 and ...
DOI:
10.1016/j.dci.2011.02.007