Subject term: humans and biotin - PubAg Search Results

1. Acute Megakaryoblastic Leukemia in One Cat and Two Dogs

Author:: F. Colbatzky; W. Hermanns
Source:: Veterinary pathology v.30 no.2 pp. 186-194
ISSN:: 1544-2217
Subject:: German Shepherd; acetates; acid phosphatase; alkaline phosphatase; animal pathology; antibodies; avidin; biotin; blood coagulation factors; blood platelets; butyrates; cats; dogs; electron microscopy; females; glycoproteins; histology; humans; lectins; leukemia; males; megakaryocytes; mixed breeds; myeloperoxidase; neoplasm cells; peroxidase
Abstract:: ... The clinical, hematologic, and histologic features of acute megakaryoblastic leukemia are described for an 8-year-old female Domestic Shorthair cat, a 3-year-old female mixed-breed dog, and a 3-year-old male German Shepherd Dog. The neoplastic cells were characterized as belonging to the megakaryocytic lineage. The following techniques were used: electron microscopy; detection of antibodies agains ...
DOI:: 10.1177/030098589303000212
: https://doi.org/10.1177/030098589303000212

2. Expression of Mononuclear Phagocyte Antigens in Histiocytic Sarcoma of Mice

Author:: J. M. Ward; W. Sheldon
Source:: Veterinary pathology v.30 no.6 pp. 560-565
ISSN:: 1544-2217
Subject:: B-lymphocytes; animal pathology; antigens; avidin; biotin; droplets; females; histology; humans; hybrids; immunoglobulin E; lectins; liver; lungs; lysozyme; macrophage colony-stimulating factor; males; mice; neoplasm cells; peroxidase; phagocytes; polyclonal antibodies; renal tubules; sarcoma; spleen; tissues; uterus
Abstract:: ... Twenty cases of histiocytic sarcoma in 15 female and five male (384 to 722 days of age) hybrid F₁(C57BL/6 x BALB/c) or F₂(F₁ x F₁) mice were studied for expression of mononuclear phagocyte and other antigens. Histiocytic sarcomas were found most often in liver, uterus, spleen, and lung. Tissues fixed in Bouin's fluid provided preservation of antigen immunoreactivity, using avidin biotin peroxidase ...
DOI:: 10.1177/030098589303000610
: https://doi.org/10.1177/030098589303000610

3. Mutational hotspot in the human biotinidase gene causes profound biotinidase deficiency

Author:: Pomponio, R.J.; Reynolds, T.R.; Cole, H.; Buck, G.A.; Wolf, B.
Source:: Nature genetics 1995 v.11 no.1 pp. 96-98
ISSN:: 1061-4036
Subject:: humans; structural genes; children; biotin; amide hydrolases; metabolism; enzyme deficiencies; vitamin deficiencies; genetic disorders; exons; alleles; mutation
DOI:: 10.1038/ng0995-96
PubMed:: 7550325
: https://doi.org/10.1038/ng0995-96

4. Structure of the human biotinidase gene

Author:: Heath Cole Knight; Thomas R. Reynolds; Gregory A. Meyers; Robert J. Pomponio; Gregory A. Buck; Barry Wolf
Source:: Mammalian genome 1998 v.9 no.4 pp. 327-330
ISSN:: 0938-8990
Subject:: bacteriophages; biotin; complementary DNA; essential genes; exons; genomic islands; humans; methylation; mutation; nucleic acid hybridization; polymerase chain reaction; promoter regions; screening
Abstract:: ... Biotinidase cleaves biotin from biocytin, thereby recycling the vitamin. We have determined the structure of the human biotinidase gene. A genomic clone, containing three exons that code for the mature enzyme, was obtained by screening a human genomic bacteriophage library with the biotinidase cDNA by plaque hybridization. To obtain a clone containing the most 5′ exon of the biotinidase cDNA, a hu ...
DOI:: 10.1007/s003359900760
: https://doi.org/10.1007/s003359900760

5. Studies on the binding site of the galactose-specific agglutinin PA-IL from Pseudomonas aeruginosa

Author:: Chie-Pein Chen; Shuh-Chyung Song; Nechama Gilboa-Garber; Kenneth S.S. Chang; Albert M. Wu
Source:: Glycobiology 1998 v.8 no.1 pp. 7-16
ISSN:: 1460-2423
Subject:: Pseudomonas aeruginosa; agglutinins; binding properties; binding sites; biotin; blood groups; glycoproteins; humans; hydrophobic bonding; lectins; ligands; melibiose; monosaccharides; polysaccharides
Abstract:: ... The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL reacted best with two glycoproteins containing Galα1→4Gal determinants and a human b ...
DOI:: 10.1093/glycob/8.1.7
: https://doi.org/10.1093/glycob/8.1.7

6. Expanding baculovirus surface display: Modification of the native coat protein gp64 of Autographa californica NPV

Author:: Wolfgang J. Ernst; Alexandra Spenger; Lars Toellner; Hermann Katinger; Reingard M. Grabherr
Source:: European journal of biochemistry 2000 v.267 no.13 pp. 4033-4039
ISSN:: 0014-2956
Subject:: Autographa californica; Autographa californica multiple nucleopolyhedrovirus; Human immunodeficiency virus 1; Western blotting; biotin; chimerism; coat proteins; epitopes; flow cytometry; humans; monoclonal antibodies; mutants; pathogenicity; peptides; virion; viruses
Abstract:: ... To create a tool for eukaryotic surface display, this approach is aimed at demonstrating a direct modification of the native envelope protein gp64 of Autographa californica NPV without disturbing viral infectivity. Short affinityâtag peptides, the biotin mimic streptagII, and the gp41 aminoâacid motif ELDKWA of HIVâ1, specific for the human monoclonal antibody 2F5, were engineered into the b ...
DOI:: 10.1046/j.1432-1327.2000.01439.x
: https://doi.org/10.1046/j.1432-1327.2000.01439.x

7. Biotinylation of histones in human cellsâ: Effects of cell proliferation

Author:: J. Steven Stanley; Jacob B. Griffin; Janos Zempleni
Source:: European journal of biochemistry 2001 v.268 no.20 pp. 5424-5429
ISSN:: 0014-2956
Subject:: biotin; biotinylation; cell cycle; cell proliferation; enzymes; histones; humans; messenger RNA
Abstract:: ... An enzymatic mechanism has been proposed by which biotinidase may catalyze biotinylation of histones. Here, human cells were found to covalently bind biotin to histones H1, H2A, H2B, H3, and H4. Cells respond to proliferation with increased biotinylation of histones; biotinylation increases early in the cell cycle and remains increased during the cycle. Notwithstanding the catalytic role of biotin ...
DOI:: 10.1046/j.0014-2956.2001.02481.x
: https://doi.org/10.1046/j.0014-2956.2001.02481.x

8. Certain Immune Markers Are Not Good Indicators of Mild to Moderate Biotin Deficiency in Rats

Author:: Helm, Ricki M.; Mock, Nell I.; Simpson, Pippa; Mock, Donald M.
Source:: Journal of nutrition 2001 v.131 no.12 pp. 3231-3236
ISSN:: 0022-3166
Subject:: immunocytes; immunoglobulin G; leucine; metabolism; phenotype; humans; immunosuppression; interferon-gamma; spleen; animal models; biotin; rats; cell proliferation; interleukin-4
Abstract:: ... To assess the effects of marginal biotin deficiency on immune function and thereby evaluate immune function as a potential marker for impaired biotin status, we investigated immune function in a rat model during progression from sufficiency to moderate biotin deficiency. As immune function indicators, we assessed the IgG response to a vaccine and the cytokine responses and relative proportions of ...
PubMed:: 11739872

9. Biotinidase catalyzes debiotinylation of histones

Author:: Tyrie D. Ballard; Jon Wolff; Jacob B. Griffin; J. Steven Stanley; Sandy van Calcar; Janos Zempleni
Source:: European journal of nutrition 2002 v.41 no.2 pp. 78-84
ISSN:: 1436-6207
Subject:: DNA repair; adults; biotin; colorimetry; enzymes; gene expression regulation; histones; humans; hydrolysis; lymphocytes; pH; patients; post-translational modification
Abstract:: ... Background Posttranslational modifications of histones play important roles in processes such as regulation of gene expression and DNA repair. Recently, evidence has been provided that histones in human cells are modified by covalent attachment of biotin. Aim of the study To determine whether the reverse process (debiotinylation of histones) occurs in biological samples and whether debiotinylation ...
DOI:: 10.1007/s003940200011
PubMed:: 12083317
: https://doi.org/10.1007/s003940200011

10. Indicators of marginal biotin deficiency and repletion in humans: validation of 3-hydroxyisovaleric acid excretion and a leucine challenge

Author:: Mock, Donald M.; Henrich, Cindy L.; Carnell, Nadine; Mock, Nell I.
Source:: American journal of clinical nutrition 2002 v.76 no.5 pp. 1061-1068
ISSN:: 0002-9165
Subject:: adults; biotin; blood; clinical trials; diet; egg albumen; excretion; gas chromatography; high performance liquid chromatography; humans; leucine; mass spectrometry; repletion; urine
Abstract:: ... Background: The results of clinical studies have provided evidence that marginal biotin deficiency is more common than was previously thought. A previous study of 10 subjects showed that the urinary excretion of biotin and 3-hydroxyisovaleric acid (3HIA) are early and sensitive indicators of marginal biotin deficiency. Objective: Marginal biotin deficiency was experimentally induced and corrected ...
PubMed:: 12399279
PubMed Central:: PMC1435357

11. Biotin Supplementation Increases Expression of Genes Encoding Interferon-γ, Interleukin-1β, and 3-Methylcrotonyl-CoA Carboxylase, and Decreases Expression of the Gene Encoding Interleukin-4 in Human Peripheral Blood Mononuclear Cells

Author:: Wiedmann, Silke; Eudy, James D.; Zempleni, Janos
Source:: Journal of nutrition 2003 v.133 no.3 pp. 716-719
ISSN:: 0022-3166
Subject:: adults; antigens; apoptosis; biotin; cell proliferation; concanavalin A; cultured cells; gene expression; gene expression regulation; genes; homeostasis; humans; immune response; interferon-gamma; interleukin-4; messenger RNA; metabolism; microarray technology; mononuclear leukocytes; reverse transcriptase polymerase chain reaction; secretion; signal transduction; stress response
Abstract:: ... Stimulation of immune cells by antigens triggers changes in the transcription of genes encoding cytokines and other proteins; these changes in gene expression are part of the normal immune response. Previous studies have provided evidence that biotin status may affect secretion of cytokines by immune cells. Here we determined whether biotin supplementation affects gene expression in human immune c ...

12. Biotin in microbes, the genes involved in its biosynthesis, its biochemical role and perspectives for biotechnological production

Author:: Streit, W.R.; Entcheva, P.
Source:: Applied microbiology and biotechnology 2003 v.61 no.1 pp. 21-31
ISSN:: 0175-7598
Subject:: biosynthesis; biotin; cosmetics; eukaryotic cells; fungi; genes; humans; synthesis; world markets
Abstract:: ... Biotin (vitamin H) is one of the most fascinating cofactors involved in central pathways in pro- and eukaryotic cell metabolism. Since its original discovery in 1901, research has led to the discovery of the complete biotin biosynthesis pathways in many different microbes and much work has been done on the highly intriguing and complex biochemistry of biotin biosynthesis. While humans and animals ...
DOI:: 10.1007/s00253-002-1186-2
PubMed:: 12658511
: https://doi.org/10.1007/s00253-002-1186-2

13. Diaminobiotin and Desthiobiotin Have Biotin-Like Activities in Jurkat Cells

Author:: Rodriguez-Melendez, Rocio; Lewis, Brandon; McMahon, Robert J.; Zempleni, Janos
Source:: Journal of nutrition 2003 v.133 no.5 pp. 1259-1264
ISSN:: 0022-3166
Subject:: biotin; culture media; cultured cells; gene expression; genes; humans; interleukin-2; luciferase; metabolites; propionyl-CoA carboxylase; transcription (genetics); transfection
Abstract:: ... In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)γ also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues are quantitatively important. Here, the hypothesis was tested that biotin metabolites can mimic the effects of b ...
PubMed:: 12730407

14. Marginal Biotin Deficiency Is Teratogenic in ICR Mice

Author:: Mock, Donald M.; Mock, Nell I.; Stewart, Christopher W.; LaBorde, James B.; Hansen, Deborah K.
Source:: Journal of nutrition 2003 v.133 no.8 pp. 2519-2525
ISSN:: 0022-3166
Subject:: abnormal development; biotin; cleft palate; diet; egg albumen; excretion; humans; mice; pregnancy; propionyl-CoA carboxylase; teratogenicity
Abstract:: ... The incidence of marginal biotin deficiency in normal human gestation is approximately one in three. In ICR mice, maternal biotin deficiency results in cleft palate, micrognathia, microglossia and limb hypoplasia. However, the relationships among the severity of maternal biotin deficiency, fetal biotin status and malformations have not been reported. This study utilized validated indices of biotin ...
PubMed:: 12888630
PubMed Central:: PMC1450011

15. Biotinylation of K12 in Histone H4 Decreases in Response to DNA Double-Strand Breaks in Human JAr Choriocarcinoma Cells

Author:: Kothapalli, Nagarama; Sarath, Gautam; Zempleni, Janos
Source:: Journal of nutrition 2005 v.135 no.10 pp. 2337-2342
ISSN:: 0022-3166
Subject:: cultured cells; carcinoma; histones; biotin; DNA damage; humans; DNA repair; chemical structure; temporal variation; lymphoma; protein phosphorylation; biochemical pathways; mortality; biotinylation
Abstract:: ... We tested the hypothesis that biotinylation of K12 in histone H4 plays a role in the cellular response to double-strand breaks (DSB) of DNA in human cells. DSB were caused by treating choriocarcinoma JAr cells with etoposide. Biotinylation of K12 in histone H4 decreased by 50% as early as 10-20 min after initiation of treatment with etoposide. Biotinylation returned to initial levels 30-40 min aft ...
Handle:: 10113/2029
PubMed:: 16177192
PubMed Central:: PMC1262666

16. High-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins in HepG2 Hepatocarcinoma Cells

Author:: Rodriguez-Melendez, Rocio; Griffin, Jacob B.; Sarath, Gautam; Zempleni, Janos
Source:: Journal of nutrition 2005 v.135 no.7 pp. 1659-1666
ISSN:: 0022-3166
Subject:: hepatocytes; transcription factors; immunoblotting; vitamin metabolism; messenger RNA; humans; signal transduction; tyrosine; gene expression; DNA-binding proteins; vitamin deficiencies; biotin; phosphotransferases (kinases); cell lines; vitamin supplements; protein composition
Abstract:: ... Biotin affects the abundance of mRNA coding for [approximately]10% of genes expressed in human-derived hepatocarcinoma (HepG2) cells. Here, we determined whether effects of biotin on gene expression are associated with changes in the abundance of distinct proteins in cell signaling and structure. HepG2 cells were cultured in media containing the following concentrations of biotin: 0.025 nmol/L (de ...
Handle:: 10113/2009
PubMed:: 15987846
PubMed Central:: PMC1224750

17. Whole-Genome Analyses of Speciation Events in Pathogenic Brucellae

Author:: Chain, Patrick S. G.; Comerci, Diego J.; Tolmasky, Marcelo E.; Larimer, Frank W.; Malfatti, Stephanie A.; Vergez, Lisa M.; Aguero, Fernan; Land, Miriam L.; Ugalde, Rodolfo A.; Garcia, Emilio
Source:: Infection and immunity 2005 v.73 no.12 pp. 8353-8361
ISSN:: 0019-9567
Subject:: Brucella melitensis biovar Abortus; DNA; NAD (coenzyme); biochemical pathways; biotin; cattle; choline; fatty acids; flagellum; glycogen; host range; host specificity; humans; multigene family; outer membrane proteins; pseudogenes; transcription (genetics); virulence
Abstract:: ... Despite their high DNA identity and a proposal to group classical Brucella species as biovars of Brucella melitensis, the commonly recognized Brucella species can be distinguished by distinct biochemical and fatty acid characters, as well as by a marked host range (e.g., Brucella suis for swine, B. melitensis for sheep and goats, and Brucella abortus for cattle). Here we present the genome of B. a ...
DOI:: 10.1128/IAI.73.12.8353-8361.2005
PubMed:: 16299333
PubMed Central:: PMC1307078
: https://doi.org/10.1128/IAI.73.12.8353-8361.2005

18. Lymphocyte propionyl-CoA carboxylase and its activation by biotin are sensitive indicators of marginal biotin deficiency in humans

Author:: Stratton, Shawna L.; Bogusiewicz, Anna; Mock, Matthew M.; Mock, Nell I.; Wells, Amanda M.; Mock, Donald M.
Source:: American journal of clinical nutrition 2006 v.84 no.2 pp. 384-388
ISSN:: 0002-9165
Subject:: humans; biotin; lymphocytes; biomarkers; vitamin deficiencies; propionyl-CoA carboxylase; enzyme activation; pregnancy; enzyme activity; blood chemistry; urine; excretion; valeric acid
Abstract:: ... BACKGROUND: Marginal biotin deficiency may be a human teratogen. A biotin status indicator that is not dependent on renal function may be useful in studies of biotin status during pregnancy. A previous study of experimental biotin deficiency suggested that propionyl-coenzyme A carboxylase (PCC) activity in peripheral blood lymphocytes (PBLs) is a sensitive indicator of biotin status. OBJECTIVE: We ...
PubMed:: 16895887
PubMed Central:: PMC1539098

19. An avidin-based assay for histone debiotinylase activity in human cell nuclei

Author:: Chew, Y.C.; Sarath, G.; Zempleni, J.
Source:: Journal of nutritional biochemistry 2007 v.18 no.7 pp. 475-481
ISSN:: 0955-2863
Subject:: avidin; biotin; histones; heterochromatin; cell cycle; animal tissues; humans; colon; lungs; placenta; lymphocytes; enzyme activity; nutrition-genotype interaction; proteolysis
Abstract:: ... The covalent binding of biotin to histones participates in heterochromatin formation, cell cycle progression and cellular response to DNA breaks. Biotinylation of histones appears to be a reversible process, but the identity of enzymes that remove biotin marks is largely unknown. Our long-term goal is to identify histone debiotinylases in human cells. Here we developed an avidin-based plate assay ...
DOI:: 10.1016/j.jnutbio.2006.09.002
PubMed:: 17156993
PubMed Central:: PMC2084399
: https://doi.org/10.1016/j.jnutbio.2006.09.002

20. K12-biotinylated histone H4 marks heterochromatin in human lymphoblastoma cells

Author:: Camporeale, G.; Oommen, A.M.; Griffin, J.B.; Sarath, G.; Zempleni, J.
Source:: Journal of nutritional biochemistry 2007 v.18 no.11 pp. 760-768
ISSN:: 0955-2863
Subject:: humans; lymphoma; biotin; biotin-binding proteins; nucleosomes; precipitin tests; heterochromatin; histones; genome; interleukin-2; transcriptional activation; promoter regions; DNA repair; phytohemagglutinin; lysine; microsatellite repeats
Abstract:: ... Covalent modifications of histones play crucial roles in chromatin structure and genomic stability. Recently, we reported a novel modification of histones: biotinylation of lysine residues. Here we provide evidence that K12-biotinylated histone H4 (K12Bio H4) maps specifically to both heterochromatin (alpha satellite repeats in pericentromeric regions) and transcriptionally repressed chromatin (γ- ...
DOI:: 10.1016/j.jnutbio.2006.12.014
PubMed:: 17434721
PubMed Central:: PMC2084386
: https://doi.org/10.1016/j.jnutbio.2006.12.014

21. Vitamin H-regulated transgene expression in mammalian cells

Author:: Weber, Wilfried; Bacchus, William; Daoud-El Baba, Marie; Fussenegger, Martin
Source:: Nucleic acids research 2007 v.35 no.17 pp. e116
ISSN:: 0305-1048
Subject:: Chinese hamsters; Escherichia coli; antibiotics; biopharmaceuticals; biotin; biotinylation; cell engineering; culture media; dimerization; gene expression; glycoproteins; hormones; humans; manufacturing; mice; models; nucleic acids; therapeutics; transactivators; transcription (genetics); transcriptional activation; transgenic animals
Abstract:: ... Although adjustable transgene expression systems are considered essential for future therapeutic and biopharmaceutical manufacturing applications, the currently available transcription control modalities all require side-effect-prone inducers such as immunosupressants, hormones and antibiotics for fine-tuning. We have designed a novel mammalian transcription-control system, which is reversibly fin ...
DOI:: 10.1093/nar/gkm466
PubMed:: 17827215
PubMed Central:: PMC2034481
: https://doi.org/10.1093/nar/gkm466

22. A novel, enigmatic histone modification: biotinylation of histones by holocarboxylase synthetase

Author:: Hassan, Yousef I.; Zempleni, Janos
Source:: Nutrition reviews 2008 v.66 no.12 pp. 721-725
ISSN:: 0029-6643
Subject:: histones; biotin; biotin-binding proteins; humans; eukaryotic cells; genes; mathematical models; fibroblasts; telomeres; Drosophila; insect models; longevity; stress tolerance; genomics; biotinylation
Abstract:: ... Holocarboxylase synthetase catalyzes the covalent binding of biotin to histones in humans and other eukaryotes. Eleven biotinylation sites have been identified in histones H2A, H3, and H4. K12-biotinylated histone H4 is enriched in heterochromatin, repeat regions, and plays a role in gene repression. About 30% of the histone H4 molecules are biotinylated at K12 in histone H4 in human fibroblast te ...
DOI:: 10.1111/j.1753-4887.2008.00127.x
PubMed:: 19019041
: https://doi.org/10.1111/j.1753-4887.2008.00127.x

23. Aptamer-based endocytosis of a lysosomal enzyme

Author:: Chen, Chi-hong B.; Dellamaggiore, Kenneth R.; Ouellette, Christopher P.; Sedano, Cecilia D.; Lizadjohry, Meikana; Chernis, George A.; Gonzales, Michelle; Baltasar, Francis E.; Fan, Audrey L.; Myerowitz, Rachel; Neufeld, Elizabeth F.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.41 pp. 15908-15913
ISSN:: 0027-8424
Subject:: DNA; biotin; calves; confocal microscopy; endocytosis; enzymes; fibroblasts; glycerol; glycosaminoglycans; humans; lysosomes; mannose; mice; oligonucleotides; oxidation; receptors; recycling; streptavidin; therapeutics; thymus gland; transfer RNA; transferrin
Abstract:: ... Enzyme replacement therapy for lysosomal storage diseases is currently based on endocytosis of lysosomal enzymes via the mannose or mannose 6-phosphate receptors. We are developing a technology for endocytosis of lysosomal enzymes that depends on generic, chemically conjugated reagents. These reagents are aptamers (single-stranded nucleic acid molecules) selected to bind to the extracellular domai ...
DOI:: 10.1073/pnas.0808360105
PubMed:: 18838694
PubMed Central:: PMC2572987
: https://doi.org/10.1073/pnas.0808360105

24. Biotinylation of Histones Represses Transposable Elements in Human and Mouse Cells and Cell Lines and in Drosophila melanogaster

Author:: Chew, Yap Ching; West, John T.; Kratzer, Stephanie J.; Ilvarsonn, Anne M.; Eissenberg, Joel C.; Dave, Bhavana J.; Klinkebiel, David; Christman, Judith K.; Zempleni, Janos
Source:: Journal of nutrition 2008 v.138 no.12 pp. 2316-2322
ISSN:: 0022-3166
Subject:: biotin; histones; transposition (genetics); retrotransposons; cell lines; humans; mice; Drosophila melanogaster; terminal repeat sequences; genome; epigenetics; gene expression regulation; lysine; neoplasms; risk factors; biotinylation
Abstract:: ... Transposable elements such as long terminal repeats (LTR) constitute ~45% of the human genome; transposition events impair genome stability. Fifty-four promoter-active retrotransposons have been identified in humans. Epigenetic mechanisms are important for transcriptional repression of retrotransposons, preventing transposition events, and abnormal regulation of genes. Here, we demonstrate that th ...
DOI:: 10.3945/jn.108.098673
PubMed:: 19022951
PubMed Central:: PMC2678950
: https://doi.org/10.3945/jn.108.098673

25. Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus

Author:: Baek, Taek Jin; Park, Pan Yun; Han, Kwi Nam; Kwon, Ho Taik; Seong, Gi Hun
Source:: Analytical and bioanalytical chemistry 2008 v.390 no.5 pp. 1373-1378
ISSN:: 1618-2642
Subject:: nanogold; electronic circuits; detection limit; semiconductors; quantitative analysis; humans; DNA; polymerase chain reaction; silver; DNA microarrays; viruses; biotin; papilloma
Abstract:: ... We describe a DNA microarray system using a bipolar integrated circuit photodiode array (PDA) chip as a new platform for DNA analysis. The PDA chip comprises an 8 x 6 array of photodiodes each with a diameter of 600 μm. Each photodiode element acts both as a support for an immobilizing probe DNA and as a two-dimensional photodetector. The usefulness of the PDA microarray platform is demonstrated b ...
DOI:: 10.1007/s00216-007-1814-x
: https://doi.org/10.1007/s00216-007-1814-x

26. Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases

Author:: Sidorenko, Viktoriya S.; Mechetin, Grigory V.; Nevinsky, Georgy A.; Zharkov, Dmitry O.
Source:: FEBS journal 2008 v.275 no.15 pp. 3747-3760
ISSN:: 1742-464X
Subject:: Escherichia coli; anions; bacteria; biotin; caffeine; chlorides; eukaryotic cells; glutamic acid; glycosylases; humans; ionic strength; magnesium; mutation; polyamides; polyamines
Abstract:: ... An abundant oxidative lesion, 8-oxo-7,8-dihydroguanine (8-oxoG), often directs the misincorporation of dAMP during replication. To prevent mutations, cells possess an enzymatic system for the removal of 8-oxoG. A key element of this system is 8-oxoguanine-DNA glycosylase (Fpg in bacteria, OGG1 in eukaryotes), which must excise 8-oxoG from 8-oxoG:C pairs but not from 8-oxoG:A. We investigated the i ...
DOI:: 10.1111/j.1742-4658.2008.06521.x
: https://doi.org/10.1111/j.1742-4658.2008.06521.x

27. gp96 Is a Human Colonocyte Plasma Membrane Binding Protein for Clostridium difficile Toxin A

Author:: Na, Xi; Kim, Ho; Moyer, Mary P.; Pothoulakis, Charalabos; LaMont, J. Thomas
Source:: Infection and immunity 2008 v.76 no.7 pp. 2862-2871
ISSN:: 0019-9567
Subject:: B-lymphocytes; Clostridium difficile; DNA fragmentation; antagonists; antibodies; apoptosis; binding proteins; biotin; cell communication; colitis; crosslinking; cytoplasm; cytotoxicity; epithelial cells; fluorescence; glycoproteins; heat shock proteins; humans; plasma membrane; precipitin tests; small interfering RNA
Abstract:: ... Clostridium difficile toxin A (TxA), a key mediator of antibiotic-associated colitis, requires binding to a cell surface receptor prior to internalization. Our aim was to identify novel plasma membrane TxA binding proteins on human colonocytes. TxA was coupled with biotin and cross-linked to the surface of HT29 human colonic epithelial cells. The main colonocyte binding protein for TxA was identif ...
DOI:: 10.1128/IAI.00326-08
PubMed:: 18411291
PubMed Central:: PMC2446715
: https://doi.org/10.1128/IAI.00326-08

28. A class of selective antibacterials derived from a protein kinase inhibitor pharmacophore

Author:: Miller, J. Richard; Dunham, Steve; Mochalkin, Igor; Banotai, Craig; Bowman, Matthew; Buist, Susan; Dunkle, Bill; Hanna, Debra; Harwood, H. James; Huband, Michael D.; Karnovsky, Alla; Kuhn, Michael; Limberakis, Chris; Liu, Jia Y.; Mehrens, Shawn; Mueller, W. Thomas; Narasimhan, Lakshmi; Ogden, Adam; Ohren, Jeff; Prasad, J.V.N. Vara; Shelly, John A.; Skerlos, Laura; Sulavik, Mark; Thomas, V. Hayden; VanderRoest, Steve; Wang, LiAnn; Wang, Zhigang; Whitton, Amy; Zhu, Tong; Stover, C. Kendall
Source:: Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.6 pp. 1737-1742
ISSN:: 0027-8424
Subject:: G-protein coupled receptors; Haemophilus influenzae; active sites; antibiotics; bacteria; biosynthesis; biotin; drug resistance; fastidious bacteria; fatty acids; genes; humans; mechanism of action; pathogens; pharmacology; protein kinases; screening
Abstract:: ... As the need for novel antibiotic classes to combat bacterial drug resistance increases, the paucity of leads resulting from target-based antibacterial screening of pharmaceutical compound libraries is of major concern. One explanation for this lack of success is that antibacterial screening efforts have not leveraged the eukaryotic bias resulting from more extensive chemistry efforts targeting euk ...
DOI:: 10.1073/pnas.0811275106
PubMed:: 19164768
PubMed Central:: PMC2644107
: https://doi.org/10.1073/pnas.0811275106

29. Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food

Author:: M. Blažková; M. Koets; J. H Wichers; A. van Amerongen; L. Fukal; P. Rauch
Source:: Czech Journal of Food Sciences v.27 no.Special Issue 1 pp. S350
ISSN:: 1805-9317
Subject:: DNA; animal pathogens; avidin; biotin; carbon; digoxigenin; fluorescein; humans; hygiene; immunoassays; immunochemistry; listeriosis
Abstract:: ... Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugat ...
DOI:: 10.17221/959-cjfs
: https://doi.org/10.17221/959-cjfs

30. Biotin interaction with human erythrocytes: contact on membrane surface and formation of self-assembled fibrous structures

Author:: K. B. Joshi; V. Venkatesh; Sandeep Verma
Source:: Chemical communications 2010 v.46 no.22 pp. 3890-3892
ISSN:: 1364-548X
Subject:: Plasmodium falciparum; atomic force microscopy; biotin; chemical communication; chemical compounds; chemical reactions; erythrocytes; humans; malaria; solutes
Abstract:: ... The binding of biotin to human erythrocytes (red blood cells, RBCs) is followed with the help of optical and atomic force microscopy methods. As certain biotin derivatives gain access to Plasmodium falciparum infected erythrocytes and block parasite-induced transport of other solutes, the microscopy observations reported in this communication will provide impetus to unravel the access mechanism of ...
DOI:: 10.1039/c001924k
: https://doi.org/10.1039/c001924k

31. Combination of quantum dot fluorescence with enzyme chemiluminescence for multiplexed detection of lung cancer biomarkers

Author:: Huan Li; Zhijuan Cao; Yuhao Zhang; Choiwan Lau; Jianzhong Lu
Source:: Analytical methods 2010 v.2 no.9 pp. 1236-1242
ISSN:: 1759-9679
Subject:: alkaline phosphatase; analytical methods; antibodies; antigens; biomarkers; biosensors; biotin; blood serum; chemiluminescence; cross reaction; detection limit; digoxin; fluorescence; humans; lung neoplasms; oligonucleotides; peroxidase; phosphopyruvate hydratase; quantum dots; streptavidin
Abstract:: ... A new concept is proposed in this article to detect multiple cancer markers in a single sample. Quantum dot (QD) fluorescence (FL) labels were successfully combined with enzyme chemiluminescence (CL) labels for simultaneous detection of three cancer markers in human serum using just a common 96-well plate reader and with equal detection limits for the three markers. As a proof-of-concept, herein w ...
DOI:: 10.1039/c0ay00284d
: https://doi.org/10.1039/c0ay00284d

32. Current understanding of the factors regulating methionine content in vegetative tissues of higher plants

Author:: Amir, Rachel
Source:: Amino acids 2010 v.39 no.4 pp. 917-931
ISSN:: 0939-4451
Subject:: S-adenosylmethionine; biotin; cells; dietary protein; enzymes; ethylene; humans; metabolism; metabolites; nutritive value; plant growth; polyamines; tissues
Abstract:: ... Methionine is a nutritionally essential, sulfur-containing amino acid found in low levels in plants, which often limits its value as a source of dietary protein to humans and animals. Methionine is also a fundamental metabolite in plant cells since, through its first metabolite, S-adenosylmethionine (SAM), it controls the level of several key metabolites, such as ethylene, polyamines and biotin. S ...
DOI:: 10.1007/s00726-010-0482-x
: https://doi.org/10.1007/s00726-010-0482-x

33. DNA microarrays for visual detection of human pathogenic microorganisms based on tyramine signal amplification coupled with gold label silver stain

Author:: Qi, Hong-Juan; Chen, Su-Hong; Zhang, Min-Li; Shi, Hua; Wang, Sheng-Qi
Source:: Analytical and bioanalytical chemistry 2010 v.398 no.6 pp. 2745-2750
ISSN:: 1618-2642
Subject:: humans; hydrogen peroxide; tyramine; silver; microorganisms; DNA microarrays; enzymatic reactions; streptavidin; biotin; computer software; catalytic activity
Abstract:: ... The utility of DNA microarrays is severely limited by their restricted sensitivity. Tyramine signal amplification (TSA) coupled with gold label silver stain (GLSS) was introduced in DNA microarrays for visual detection of human pathogenic microorganisms. First, a TSA system was introduced to the microarrays after the microarrays were prepared and hybridized with biotinylated targets. This procedur ...
DOI:: 10.1007/s00216-010-4189-3
: https://doi.org/10.1007/s00216-010-4189-3

34. K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts

Author:: Wijeratne, Subhashinee S.K.; Camporeale, Gabriela; Zempleni, Janos
Source:: Journal of nutritional biochemistry 2010 v.21 no.4 pp. 310-316
ISSN:: 0955-2863
Subject:: human cell lines; nutrient transport; telomeres; humans; biotin; histones; cell culture; nutrition-genotype interaction; gene overexpression; biotinylation; precipitin tests; vitamin supplements; lungs; fibroblasts; biotin-binding proteins; chromatin
Abstract:: ... Covalent modifications of histones play a role in regulating telomere attrition and cellular senescence. Biotinylation of lysine (K) residues in histones, mediated by holocarboxylase synthetase (HCS), is a novel diet-dependent mechanism to regulate chromatin structure and gene expression. We have previously shown that biotinylation of K12 in histone H4 (H4K12bio) is a marker for heterochromatin an ...
DOI:: 10.1016/j.jnutbio.2009.01.010
PubMed:: 19369050
PubMed Central:: PMC2854078
: https://doi.org/10.1016/j.jnutbio.2009.01.010

35. Plasma concentration of 3-hydroxyisovaleryl carnitine is an early and sensitive indicator of marginal biotin deficiency in humans

Author:: Stratton, Shawna L.; Horvath, Thomas D.; Bogusiewicz, Anna; Matthews, Nell I.; Henrich, Cindy L.; Spencer, Horace J.; Moran, Jeffery H.; Mock, Donald M.
Source:: American journal of clinical nutrition 2010 v.92 no.6 pp. 1399-1405
ISSN:: 0002-9165
Subject:: vitamin metabolism; carnitine; nutritional status; humans; methodology; vitamin deficiencies; biotin; blood plasma; nutrition assessment
Abstract:: ... BACKGROUND: Blood-based indicators of biotin status in humans were shown to be useful tools in several clinical situations, including pregnancy. We previously validated the activity of the biotin-dependent enzyme propionyl-coenzyme A carboxylase (PCC) in lymphocytes as a sensitive and specific blood-based indicator of marginal degrees of biotin deficiency. However, the measurement of PCC activity ...
DOI:: 10.3945/ajcn.110.002543
PubMed:: 20943794
PubMed Central:: PMC2980966
: https://doi.org/10.3945/ajcn.110.002543

36. Short Oligonucleotide Tandem Ligation Assay for Genotyping of Single-Nucleotide Polymorphisms in Y Chromosome

Author:: Skobeltsyna, Larisa M.; Pyshnyi, Dmitrii V.; Ivanova, Eugenia M.; Stepanov, Vadim A.; Puzyrev, Valery P.; Dymshits, Grigory M.; Kharkov, Vladimir N.; Zarytova, Valentina F.
Source:: Molecular biotechnology 2010 v.45 no.1 pp. 1-8
ISSN:: 1073-6085
Subject:: Y chromosome; binding sites; biotin; complementary DNA; genotyping; humans; oligonucleotides; polymerase chain reaction; polymers; restriction fragment length polymorphism; single nucleotide polymorphism
Abstract:: ... We propose a novel universal methodology, Short Oligonucleotide Tandem Ligation Assay (SOTLA), for SNP genotyping. SOTLA is based on using a tandem of short oligonucleotide (TSO) probes consisting of three fragments: the core oligonucleotide and two flanking oligomers, one of which is immobilized onto a solid support and another one contains the biotin label. TSO is self-associated on a complement ...
DOI:: 10.1007/s12033-009-9208-2
: https://doi.org/10.1007/s12033-009-9208-2

37. Studying copper(ii) ion induced interactions of β-amyloid peptides within living cells by gold nanoparticle probes

Author:: Chengke Wang; Jine Wang; Dianjun Liu; Zhenxin Wang
Source:: Analytical methods 2010 v.2 no.10 pp. 1467-1471
ISSN:: 1759-9679
Subject:: EDTA (chelating agent); biotin; chelating agents; color; colorimetry; copper; ethylene glycol tetraacetic acid; glioma; histidine; humans; nanogold; peptides; streptavidin
Abstract:: ... In this paper, a kind of gold nanoparticle (GNP)-based colorimetric assay was developed for studying copper(ii) ion (Cu²⁺) induced interaction of β-amyloid (Aβ) peptide with living cells. In the presence of Cu²⁺, biotin modified β-amyloid peptides (Aβ₁–₁₆(biotin)) enable to attach on the SHG-44 (human glioma cell) cellular surface which results in significant color change of the cells when the ind ...
DOI:: 10.1039/c0ay00438c
: https://doi.org/10.1039/c0ay00438c

38. A Biotin Switch-Based Proteomics Approach Identifies 14-3-3ζ as a Target of Sirt1 in the Metabolic Regulation of Caspase-2

Author:: Joshua L. Andersen; J. Will Thompson; Kelly R. Lindblom; Erika S. Johnson; Chih-Sheng Yang; Lauren R. Lilley; Christopher D. Freel; M. Arthur Moseley; Sally Kornbluth
Source:: Molecular cell 2011 v.43 no.5 pp. 834-842
ISSN:: 1097-2765
Subject:: Xenopus; acetylation; apoptosis; biotin; caspase-2; cultured cells; death; dissociation; eggs; glucose 6-phosphate; humans; lysine; proteomics
Abstract:: ... While lysine acetylation in the nucleus is well characterized, comparatively little is known about its significance in cytoplasmic signaling. Here we show that inhibition of the Sirt1 deacetylase, which is primarily cytoplasmic in cancer cell lines, sensitizes these cells to caspase-2-dependent death. To identify relevant Sirt1 substrates, we developed a proteomics strategy, enabling the identific ...
DOI:: 10.1016/j.molcel.2011.07.028
PubMed:: 21884983
PubMed Central:: PMC3417139
: https://doi.org/10.1016/j.molcel.2011.07.028

39. A method of printing uniform protein lines by using flat PDMS stamps

Author:: Wei Zhang; Chang-Ying Xue; Kun-Lin Yang
Source:: Journal of colloid and interface science 2011 v.353 no.1 pp. 143-148
ISSN:: 0021-9797
Subject:: biotin; bovine serum albumin; glass; humans; immunoassays; immunoglobulin G; irradiation; lamps; mice; ultraviolet radiation
Abstract:: ... In this paper, we report a method of printing uniform protein lines on glass slides by using UV-treated flat PDMS stamps. Unlike traditional microcontact printing (μCP) which requires microstructured PDMS stamps, this μCP method only requires a flat PDMS stamp, an UV lamp and a number of straight needles. Our results show that lines of bovine serum albumin (BSA), immunoglobin (IgG), anti-biotin, a ...
DOI:: 10.1016/j.jcis.2010.09.029
PubMed:: 20933241
: https://doi.org/10.1016/j.jcis.2010.09.029

40. A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure

Author:: Norihiro Kobayashi; Erika Banzono; Yuko Shimoda; Hiroyuki Oyama; Toshiomi Kunihiro; Izumi Morita; Mitsuhiro Ohta
Source:: Analytical methods 2011 v.3 no.9 pp. 1995-2002
ISSN:: 1759-9679
Subject:: amino acid sequences; analytical methods; biotin; chloroform; cotinine; cross reaction; dose response; enzyme-linked immunosorbent assay; humans; hybridomas; immunoglobulin G; metabolites; mice; monoclonal antibodies; myeloma; nicotine; nitrogen oxides; smoke; splenocytes; streptavidin; tobacco; urine
Abstract:: ... Immunoassays for human urinary cotinine [(S)-(−)-cotinine], a major nicotine metabolite, are useful to monitor the degree of tobacco smoke exposure. However, practical monoclonal antibodies for measuring urinary cotinine are not widely available. Here we generated a monoclonal antibody against a newly prepared cotinine–albumin conjugate and developed an enzyme-linked immunosorbent assay (ELISA). S ...
DOI:: 10.1039/c1ay05083d
: https://doi.org/10.1039/c1ay05083d

41. Carbon nano-strings as reporters in lateral flow devices for DNA sensing by hybridization

Author:: Kalogianni, Despina P.; Boutsika, Lemonia M.; Kouremenou, Panagiota G.; Christopoulos, Theodore K.; Ioannou, Penelope C.
Source:: Analytical and bioanalytical chemistry 2011 v.400 no.4 pp. 1145-1152
ISSN:: 1618-2642
Subject:: DNA; DNA-directed DNA polymerase; biotin; carbon; genotyping; humans; immunoaffinity chromatography; nanoparticles; nucleic acid hybridization; nucleotide sequences; polymerase chain reaction; single nucleotide polymorphism; streptavidin; washing
Abstract:: ... Presently, there is a growing interest in the development of lateral flow devices for nucleic acid analysis that enable visual detection of the target sequence (analyte) while eliminating several steps required for pipetting, incubation, and washing out the excess of reactants. In this paper, we present, for the first time, lateral flow tests exploiting oligonucleotide-functionalized and antibody- ...
DOI:: 10.1007/s00216-011-4845-2
: https://doi.org/10.1007/s00216-011-4845-2

42. Design, recombinant expression and in vitro maturation of human insulin-like peptide 6 and a biotin-labeled analogue

Author:: Xiao Luo; Chun-Hua Huang; Xiao-Xia Shao; Zhan-Yun Guo
Source:: Process biochemistry 2011 v.46 no.6 pp. 1243-1247
ISSN:: 1359-5113
Subject:: Escherichia coli; biotin; circular dichroism spectroscopy; cyanogen; humans; hyperplasia; insulin; relaxin; spermatogenesis; testes
Abstract:: ... Insulin-like peptide 6 (INSL6) is a newly identified peptide hormone belonging to the insulin/relaxin superfamily. It is predominantly expressed in the testes and responsible for spermatogenesis. It also shows potential for the treatment of benign prostate hyperplasia. For further basic and preclinical studies, significant quantities of INSL6 peptide are needed. In the present work, we designed an ...
DOI:: 10.1016/j.procbio.2011.02.011
: https://doi.org/10.1016/j.procbio.2011.02.011

43. Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum

Author:: Hoyoung Park; Insook Rhee Paeng
Source:: Analytica chimica acta 2011 v.685 no.1 pp. 65-73
ISSN:: 0003-2670
Subject:: RNA; absorbance; analytical specificity; biotin; blood serum; chemical analysis; correlation; cost effectiveness; cross reaction; detection limit; dialysis; dopamine; enzyme-linked immunosorbent assay; epinephrine; homovanillic acid; humans; magnesium; monitoring; neurotransmitters; norepinephrine; oligonucleotides; proteins; screening; temperature
Abstract:: ... A rapid and cost-effective screening method based on a competitive enzyme-linked aptamer assay (ELAA) for dopamine (DA) in serum has been optimized and validated. In this paper, we report advantageous sensitivity and specificity of aptamer assays as compared to the existing antibody based-immunoassays. The RNA aptamer (67 mer) was immobilized via site-directed immobilization with biotin both at th ...
DOI:: 10.1016/j.aca.2010.11.010
PubMed:: 21168553
: https://doi.org/10.1016/j.aca.2010.11.010

44. Enzyme-amplified electrochemical biosensor for detection of PML–RARα fusion gene based on hairpin LNA probe

Author:: Liqing Lin; Qicai Liu; Liman Wang; Ailin Liu; Shaohuang Weng; Yun Lei; Wei Chen; Xinhua Lin; Yuanzhong Chen
Source:: Biosensors & bioelectronics 2011 v.28 no.1 pp. 277-283
ISSN:: 0956-5663
Subject:: DNA; biosensors; biotin; blood serum; electrochemistry; electrodes; gene fusion; humans; leukemia; nucleic acid hybridization; peroxidase; polymerase chain reaction; retinoic acid
Abstract:: ... In this study, an enzyme-amplified electrochemical biosensor was developed for detection of the promyelocytic leukemia/retinoic acid receptor alpha (PML/RARα) fusion gene in acute promyelocytic leukemia (APL). This new sensor employs a hairpin locked nucleic acids (LNAs) probe dually labeled with biotin and carboxyfluorescein molecule (FAM). The probe is immobilized at a streptavidin-modified elec ...
DOI:: 10.1016/j.bios.2011.07.032
: https://doi.org/10.1016/j.bios.2011.07.032

45. Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18

Author:: Baolong Bao; Valerie Pestinger; Yousef I. Hassan; Gloria E.O. Borgstahl; Carol Kolar; Janos Zempleni
Source:: Journal of nutritional biochemistry 2011 v.22 no.5 pp. 470-475
ISSN:: 0955-2863
Subject:: RNA; antibodies; bioactive properties; biotin; biotinylation; chromatin; genes; histones; humans; longevity; lysine; nucleotide sequences; peptides; proteolysis; streptavidin; stress tolerance; yeasts
Abstract:: ... Holocarboxylase synthetase (HCS) mediates the binding of biotin to lysine (K) residues in histones H2A, H3 and H4; HCS knockdown disturbs gene regulation and decreases stress resistance and lifespan in eukaryotes. We tested the hypothesis that HCS interacts physically with histone H3 for subsequent biotinylation. Co-immunoprecipitation experiments were conducted and provided evidence that HCS co-l ...
DOI:: 10.1016/j.jnutbio.2010.04.001
PubMed:: 20688500
PubMed Central:: PMC2975038
: https://doi.org/10.1016/j.jnutbio.2010.04.001

46. Human holocarboxylase synthetase with a start site at methionine-58 is the predominant nuclear variant of this protein and has catalytic activity

Author:: Baolong Bao; Subhashinee S.K. Wijeratne; Rocio Rodriguez-Melendez; Janos Zempleni
Source:: Biochemical and biophysical research communications 2011 v.412 no.1 pp. 115-120
ISSN:: 0006-291X
Subject:: Western blotting; bioactive properties; biotin; biotinylation; catalytic activity; chromatin; confocal microscopy; cytoplasm; epigenetics; genes; histones; humans; metabolism; multiprotein complexes; nuclear localization signals; translation (genetics)
Abstract:: ... Holocarboxylase synthetase (HLCS) catalyzes the covalent binding of biotin to both carboxylases in extranuclear structures and histones in cell nuclei, thereby mediating important roles in intermediary metabolism, gene regulation, and genome stability. HLCS has three putative translational start sites (methionine-1, -7, and -58), but lacks a strong nuclear localization sequence that would explain ...
DOI:: 10.1016/j.bbrc.2011.07.055
PubMed:: 21802411
PubMed Central:: PMC3159853
: https://doi.org/10.1016/j.bbrc.2011.07.055

47. Identification of holocarboxylase synthetase chromatin binding sites in human mammary cell lines using the DNA adenine methyltransferase identification technology

Author:: Dipika Singh; Angela K. Pannier; Janos Zempleni
Source:: Analytical biochemistry 2011 v.413 no.1 pp. 55-59
ISSN:: 0003-2697
Subject:: DNA; adenine; binding sites; biogenesis; biotin; biotinylation; breast neoplasms; chromatin; corticotropin; digestion; genes; histones; human cell lines; humans; leptin; methylation; methyltransferases; microarray technology; myo-inositol; regulatory proteins; ribosomes; transfection
Abstract:: ... Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS ...
DOI:: 10.1016/j.ab.2011.02.001
PubMed:: 21303649
PubMed Central:: PMC3070904
: https://doi.org/10.1016/j.ab.2011.02.001

48. In vivo biotinylated recombinant influenza A virus hemagglutinin for use in subtype-specific serodiagnostic assays

Author:: Alexander Postel; Tobias Letzel; Frederik Müller; Ralf Ehricht; Philippe Pourquier; Malte Dauber; Christian Grund; Martin Beer; Timm C. Harder
Source:: Analytical biochemistry 2011 v.411 no.1 pp. 22-31
ISSN:: 0003-2697
Subject:: Baculoviridae; Orthomyxoviridae; biotin; biotinylation; endoplasmic reticulum; enzyme-linked immunosorbent assay; epitopes; hemagglutination; hemagglutinins; humans; influenza; insects; membrane proteins; monoclonal antibodies; poultry; recombinant antigens; serodiagnosis; viruses
Abstract:: ... There is an urgent need for robust subtype-specific serological tests to diagnose influenza A virus infections in poultry and mammals, including humans. Such assays require reliable subtype-specific sources of soluble and authentically folded seroreactive hemagglutinin (HA), one of the integral membrane proteins that determine the serological subtype of influenza viruses. To this purpose, a bigeni ...
DOI:: 10.1016/j.ab.2010.12.022
: https://doi.org/10.1016/j.ab.2010.12.022

49. Loss of Taste Responds to High-Dose Biotin Treatment

Author:: Frank L Greenway; Donald K Ingram; Eric Ravussin; Mark Hausmann; Steven R Smith; Lauren Cox; Katie Tomayko; Benjamin V Treadwell
Source:: Journal of the American College of Nutrition 2011 v.30 no.3 pp. 178-181
ISSN:: 0731-5724
Subject:: biotin; calcium; case studies; dietary supplements; humans; lipoic acid; obesity; patients; phosphorus; quality of life; taste; transporters; urine; women
Abstract:: ... BACKGROUND AND OBJECTIVE: We saw 2 patients who lost their sense of taste, which was restored by pharmacologic doses of biotin. The key objective is to describe the 2 case reports and suggest a potential treatment for unexplained loss of taste. METHODS AND DESIGN: The first patient was a 67-year-old woman who lost her sense of taste taking Juvenon, a dietary herbal supplement containing acyl-L-car ...
DOI:: 10.1080/07315724.2011.10719958
: https://doi.org/10.1080/07315724.2011.10719958

50. Multienzyme-nanoparticles amplification for sensitive virus genotyping in microfluidic microbeads array using Au nanoparticle probes and quantum dots as labels

Author:: He Zhang; Lian Liu; Cheuk-Wing Li; Huayang Fu; Yao Chen; Mengsu Yang
Source:: Biosensors & bioelectronics 2011 v.29 no.1 pp. 89-96
ISSN:: 0956-5663
Subject:: DNA; Papillomaviridae; biosensors; biotin; genotype; genotyping; gold; humans; hydrogen peroxide; nanogold; nanoparticles; nucleic acid hybridization; oligonucleotides; oxidation; peroxidase; polymerase chain reaction; proteins; quantum dots; surface area; viruses
Abstract:: ... A novel microfluidic device with microbeads array was developed and sensitive genotyping of human papillomavirus was demonstrated using a multiple-enzyme labeled oligonucleotide–Au nanoparticle bioconjugate as the detection tool. This method utilizes microbeads as sensing platform that was functionalized with the capture probes and modified electron rich proteins, and uses the horseradish peroxida ...
DOI:: 10.1016/j.bios.2011.07.074
: https://doi.org/10.1016/j.bios.2011.07.074

51. Nanoparticle-based electrochemiluminescence immunosensor with enhanced sensitivity for cardiac troponin I using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles as labels

Author:: Wen Shen; Dayong Tian; Hua Cui; Di Yang; Zhiping Bian
Source:: Biosensors & bioelectronics 2011 v.27 no.1 pp. 18-24
ISSN:: 0956-5663
Subject:: antibodies; biomarkers; biotin; detection limit; disease diagnosis; electrodes; humans; immunoassays; immunosensors; luminescence; myocardial infarction; nanogold; stabilizers; streptavidin
Abstract:: ... A novel nanoparticle-based electrochemiluminescence (ECL) immunosensor was designed for highly sensitive and selective detection of human cardiac troponin I (cTnI), an important Acute Myocardial Infarction (AMI)-related biomarker, by using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles (ABEI-AuNPs) as labels. ABEI-AuNPs were successfully synthesized via a simple seed growth m ...
DOI:: 10.1016/j.bios.2011.05.022
: https://doi.org/10.1016/j.bios.2011.05.022

52. Oxidative stress induced carbonylation in human plasma

Author:: Ashraf G. Madian; Naomi Diaz-Maldonado; Qiang Gao; Fred E. Regnier
Source:: Journal of proteomics 2011 v.74 no.11 pp. 2395-2416
ISSN:: 1874-3919
Subject:: biotin; blood; blood proteins; breast neoplasms; humans; immunity; isotopes; nucleic acids; oxidative stress; patients
Abstract:: ... The focus of this study was on the assessment of technology that might be of clinical utility in identification, quantification, characterization of carbonylation in human plasma proteins. Carbonylation is widely associated with oxidative stress diseases. Breast cancer patient samples were chosen as a stress positive case based on the fact that oxidative stress has been reported to be elevated in ...
DOI:: 10.1016/j.jprot.2011.07.014
PubMed:: 21856457
PubMed Central:: PMC3199367
: https://doi.org/10.1016/j.jprot.2011.07.014

53. Proteomic profiling of acrolein adducts in human lung epithelial cells

Author:: Page C. Spiess; Bin Deng; Robert J. Hondal; Dwight E. Matthews; Albert van der Vliet
Source:: Journal of proteomics 2011 v.74 no.11 pp. 2380-2394
ISSN:: 1874-3919
Subject:: acrolein; adverse effects; avidin; biotin; combustion; cytoskeletal proteins; enzyme inactivation; epithelial cells; epithelium; glutathione transferase; humans; peptides; proteomics; selenocysteine; smoke; tobacco
Abstract:: ... Acrolein (2,3-propenal) is a major indoor and outdoor air pollutant originating largely from tobacco smoke or organic combustion. Given its high reactivity, the adverse effects of inhaled acrolein are likely due to direct interactions with the airway epithelium, resulting in altered epithelial function, but only limited information exists to date regarding the primary direct cellular targets for a ...
DOI:: 10.1016/j.jprot.2011.05.039
PubMed:: 21704744
PubMed Central:: PMC3196826
: https://doi.org/10.1016/j.jprot.2011.05.039

54. Replication of biotinylated human immunodeficiency viruses

Author:: Michael Belshan; John M. Matthews; Christian J. Madson
Source:: Journal of virological methods 2011 v.171 no.1 pp. 299-302
ISSN:: 0166-0934
Subject:: virion; Human immunodeficiency virus 1; biotinylation; humans; immunosuppression; Human immunodeficiency virus 2; viral proteins; genes; viruses; biotin; virus replication; Escherichia coli
Abstract:: ... Previous work demonstrated recently the adaptation of the Escherichia coli biotin ligase BirA – biotin acceptor sequence (BAS) labeling system to produce human immunodeficiency virus type 1 viruses with biotinylated integrase (NLXINB) and matrix (NLXMAB) proteins (Belshan et al., 2009). This report describes the construction of an HIV permissive cell line stably expressing BirA (SupT1.BirA). Consi ...
DOI:: 10.1016/j.jviromet.2010.11.005
PubMed:: 21087640
PubMed Central:: PMC3563423
: https://doi.org/10.1016/j.jviromet.2010.11.005

55. Site-Specific Coupling and Sterically Controlled Formation of Multimeric Antibody Fab Fragments with Unnatural Amino Acids

Author:: Benjamin M. Hutchins; Stephanie A. Kazane; Karin Staflin; Jane S. Forsyth; Brunhilde Felding-Habermann; Peter G. Schultz; Vaughn V. Smider
Source:: Journal of molecular biology 2011 v.406 no.4 pp. 595-603
ISSN:: 0022-2836
Subject:: amino acids; antibodies; antigens; biotin; biotin-binding proteins; biotinylation; breast neoplasms; epidermal growth factor receptors; fermentation; humans; phosphorylation; therapeutics
Abstract:: ... Immunoconjugates and multispecific antibodies are rapidly emerging as highly potent experimental therapeutics against cancer. We have developed a method to incorporate an unnatural amino acid, p-acetylphenylalanine (pAcPhe) into an antibody antigen binding fragment (Fab) targeting HER2 (human epidermal growth factor receptor 2), allowing site-specific labeling without disrupting antigen binding. E ...
DOI:: 10.1016/j.jmb.2011.01.011
PubMed:: 21237172
PubMed Central:: PMC4278757
: https://doi.org/10.1016/j.jmb.2011.01.011

56. Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast

Author:: Pluskal, Tomáš; Hayashi, Takeshi; Saitoh, Shigeaki; Fujisawa, Asuka; Yanagida, Mitsuhiro
Source:: FEBS journal 2011 v.278 no.8 pp. 1299-1315
ISSN:: 1742-464X
Subject:: glutathione; energy; longevity; humans; biomarkers; glucose; hydrogen peroxide; Schizosaccharomyces pombe; blood glucose; adenosine triphosphate; cell division; fasting; trehalose; metabolomics; methionine; biotin; metabolites; starvation
Abstract:: ... Glucose as a source of energy is centrally important to our understanding of life. We investigated the cell division-quiescence behavior of the fission yeast Schizosaccharomyces pombe under a wide range of glucose concentrations (0-111 mm). The mode of S. pombe cell division under a microfluidic perfusion system was surprisingly normal under highly diluted glucose concentrations (5.6 mm, 1/20 of t ...
DOI:: 10.1111/j.1742-4658.2011.08050.x
PubMed:: 21306563
PubMed Central:: PMC3123465
: https://doi.org/10.1111/j.1742-4658.2011.08050.x

57. Urinary Excretion of 3-Hydroxyisovaleric Acid and 3-Hydroxyisovaleryl Carnitine Increases in Response to a Leucine Challenge in Marginally Biotin-Deficient Humans

Author:: Donald M. Mock; Shawna L. Stratton; Thomas D. Horvath; Anna Bogusiewicz; Nell I. Matthews; Cindy L. Henrich; Amanda M. Dawson; Horace J. Spencer; Suzanne N. Owen; Gunnar Boysen; Jeffery H. Moran
Source:: Journal of nutrition 2011 v.141 no.11 pp. 1925-1930
ISSN:: 0022-3166
Subject:: acids; biochemical pathways; biotin; carnitine; diagnostic sensitivity; egg albumen; enzymes; excretion; humans; leucine; metabolites
Abstract:: ... Experimentally increasing metabolic flux in a pathway in which an essential step is catalyzed by a vitamin-dependent enzyme (a challenge test) has been used in assessing functional vitamin status and elucidating common and alternate metabolic pathways. Conversion of 3-methylcrotonyl CoA to 3-methylglutaconyl CoA in the leucine catabolic pathway is catalyzed by the biotin-dependent enzyme methylcro ...
DOI:: 10.3945/jn.111.146126
PubMed:: 21918059
PubMed Central:: PMC3192457
: https://doi.org/10.3945/jn.111.146126

58. Urinary Excretion of 3-Hydroxyisovaleryl Carnitine Is an Early and Sensitive Indicator of Marginal Biotin Deficiency in Humans

Author:: Stratton, Shawna L.; Horvath, Thomas D.; Bogusiewicz, Anna; Matthews, Nell I.; Henrich, Cindy L.; Spencer, Horace J.; Moran, Jeffery H.; Mock, Donald M.
Source:: Journal of nutrition 2011 v.141 no.3 pp. 353-358
ISSN:: 0022-3166
Subject:: repletion; diet; carnitine; urine; humans; women; excretion; methodology; creatinine; biotin; egg albumen; adults
Abstract:: ... Mounting evidence indicates that marginal biotin deficiency is not rare, contrary to previous assumptions. Accordingly, robust indicators of biotin status would be useful. In a study of 10 healthy adults, we recently provided evidence that abnormally increased plasma concentration of 3-hydroxyisovaleryl carnitine (3HIA-carnitine) is a sensitive indicator of marginal biotin deficiency. We sought to ...
DOI:: 10.3945/jn.110.135772
PubMed:: 21248194
PubMed Central:: PMC3040900
: https://doi.org/10.3945/jn.110.135772

59. Aluminum Oxide Nanoparticles as Carriers and Adjuvants for Eliciting Antibodies from Non-immunogenic Haptens

Author:: Maquieira Ángel; Brun Eva M.; Garcés-García Marta; Puchades Rosa
Source:: Analytical chemistry 2012 v.84 no.21 pp. 9340-9348
ISSN:: 1520-6882
Subject:: adjuvants; aluminum oxide; antibiotics; antibodies; atrazine; biotin; cross reaction; crystal structure; derivatization; enzyme-linked immunosorbent assay; haptens; humans; immune system; immunoglobulin G; mice; molecular weight; nanoparticles; rabbits; sulfasalazine; transport proteins; vaccination
Abstract:: ... Functionalized nanomaterials have applications yet to be discovered, especially in the biological field and, in particular, the in vivo production of antibodies. In this paper, we show that aluminum oxide nanoparticles that are covalently coupled to haptens (small molecular mass compounds) activate the immune system, eliciting antibodies (polyclonal and monoclonal) specific for the herbicide atraz ...
DOI:: 10.1021/ac3020998
: https://doi.org/10.1021/ac3020998

60. Avidin Functionalized Maghemite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification

Author:: Magro Massimiliano; Faralli Adele; Baratella Davide; Bertipaglia Ilenia; Giannetti Sara; Salviulo Gabriella; Zboril Radek; Vianello Fabio
Source:: Langmuir 2012 v.28 no.43 pp. 15392-15401
ISSN:: 1520-5827
Subject:: Ca2-transporting ATPase; Fourier transform infrared spectroscopy; Saccharomyces cerevisiae; absorbance; adsorption; aqueous solutions; avidin; binding capacity; binding sites; bioactive properties; biotin; colloidal properties; endoplasmic reticulum; humans; iron; ligands; maghemite; magnetism; models; nanoparticles; quartz crystal microbalance; sorption isotherms; surface interactions; titration; transmission electron microscopy; ultraviolet-visible spectroscopy; yeasts
Abstract:: ... We report on the surface characterization, functionalization, and application of stable water suspensions of novel surface active maghemite nanoparticles (SAMNs), characterized by a diameter of 11 ± 2 nm and possessing peculiar colloidal properties and surface interactions. These features permitted the acquisition of titration curves and aqueous UV–vis spectra and suggested a role played by surfac ...
DOI:: 10.1021/la303148u
: https://doi.org/10.1021/la303148u

61. Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system

Author:: Huanshun Yin; Yunlei Zhou; Haixia Zhang; Xiaomeng Meng; Shiyun Ai
Source:: Biosensors & bioelectronics 2012 v.33 no.1 pp. 247-253
ISSN:: 0956-5663
Subject:: DNA; barcoding; benzoquinones; biosensors; biotin; detection limit; electrochemistry; glassy carbon electrode; gold; graphene; humans; hydrogen peroxide; hydroquinone; microRNA; nanogold; neoplasms; non-coding RNA; nucleic acid hybridization; nucleotides; oxidation
Abstract:: ... MicroRNAs (miRNAs), a kind of small, endogenous, noncoding RNAs (∼22 nucleotides), might play a crucial role in early cancer diagnose due to its abnormal expression in many solid tumors. As a result, label-free and PCR-amplification-free assay for miRNAs is of great significance. In this work, a highly sensitive biosensor for sequence specific miRNA-21 detection without miRNA-21 labeling and enric ...
DOI:: 10.1016/j.bios.2012.01.014
: https://doi.org/10.1016/j.bios.2012.01.014

62. Endogenously produced nitric oxide mitigates sensitivity of melanoma cells to cisplatin

Author:: Luiz C. Godoy; Chase T. M. Anderson; Rajdeep Chowdhury; Laura J. Trudel; Gerald N. Wogan
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.50 pp. 20373-20378
ISSN:: 0027-8424
Subject:: adjuvants; chemiluminescence; drug therapy; humans; cisplatin; melanoma; cytotoxicity; nitric oxide; inducible nitric oxide synthase; patients; biotin; hypoxia-inducible factor 1; apoptosis; caspase-3
Abstract:: ... Melanoma patients experience inferior survival after biochemotherapy when their tumors contain numerous cells expressing the inducible isoform of NO synthase (iNOS) and elevated levels of nitrotyrosine, a product derived from NO. Although several lines of evidence suggest that NO promotes tumor growth and increases resistance to chemotherapy, it is unclear how it shapes these outcomes. Here we dem ...
DOI:: 10.1073/pnas.1218938109
PubMed:: 23185001
PubMed Central:: PMC3528548
: https://doi.org/10.1073/pnas.1218938109

63. Engineering of novel tamavidin 2 muteins with lowered isoelectric points and lowered non-specific binding properties

Author:: Yoshimitsu Takakura; Naomi Oka; Hitomi Kajiwara; Masako Tsunashima
Source:: Journal of bioscience and bioengineering 2012 v.114 no.5 pp. 485-489
ISSN:: 1389-1723
Subject:: DNA; Escherichia coli; arginine; avidin; binding properties; biotin; engineering; fibronectins; fungi; glutamic acid; glycoproteins; humans; immunoglobulin G; integrins; isoelectric point; lysine; mutants; recombinant proteins; salmon; spermatozoa; streptavidin
Abstract:: ... The avidin–biotin interaction is widely employed as a universal tool in numerous biotechnological applications. In avidin–biotin technology, non-specific binding to biological macromolecules is a hindrance. The major origin of this non-specific binding is the electrical charge of the surface of biotin-binding proteins. Tamavidin 2, a fungal avidin-like protein that binds biotin with an extremely h ...
DOI:: 10.1016/j.jbiosc.2012.06.009
: https://doi.org/10.1016/j.jbiosc.2012.06.009

64. Enzyme-Linked Small-Molecule Detection Using Split Aptamer Ligation

Author:: Sharma Ashwani K.; Kent Alexandra D.; Heemstra Jennifer M.
Source:: Analytical chemistry 2012 v.84 no.14 pp. 6104-6109
ISSN:: 1520-6882
Subject:: DNA; biotin; blood serum; cocaine; colorimetry; detection limit; enzyme-linked immunosorbent assay; humans; oligonucleotides; peroxidase
Abstract:: ... Here we report an aptamer-based analogue of the widely used sandwich enzyme-linked immunosorbent assay (ELISA). This assay utilizes the cocaine split aptamer, which is comprised of two DNA strands that only assemble in the presence of the target small molecule. One split aptamer fragment is immobilized on a microplate, then a test sample is added containing the second split aptamer fragment. If co ...
DOI:: 10.1021/ac300997q
: https://doi.org/10.1021/ac300997q

65. Identification and quantification of newly synthesized proteins translationally regulated by YB-1 using a novel Click–SILAC approach

Author:: Syam Prakash Somasekharan; Nikolay Stoynov; Barak Rotblat; Gabriel Leprivier; Jason D. Galpin; Christopher A. Ahern; Leonard J. Foster; Poul H.B. Sorensen
Source:: Journal of proteomics 2012 v.77 no. pp. e1
ISSN:: 1874-3919
Subject:: bioinformatics; biotin; cell culture; chemistry; humans; isotope labeling; mass spectrometry; methionine; protein synthesis; proteome; regulatory proteins; sarcoma; small interfering RNA; stable isotopes
Abstract:: ... Messenger RNA-binding translational regulatory proteins determine in large part the spectrum of transcripts that are translated under specific cellular contexts. Y-box binding protein-1 (YB-1) is a conserved eukaryotic translational regulator that is implicated in cancer progression. To identify specific proteins that are translationally regulated by YB-1, we established a pulse-labelling approach ...
DOI:: 10.1016/j.jprot.2012.08.019
PubMed:: 22967496
: https://doi.org/10.1016/j.jprot.2012.08.019

66. K16-biotinylated histone H4 is overrepresented in repeat regions and participates in the repression of transcriptionally competent genes in human Jurkat lymphoid cells

Author:: Luisa Rios-Avila; Valerie Pestinger; Janos Zempleni
Source:: Journal of nutritional biochemistry 2012 v.23 no.12 pp. 1559-1564
ISSN:: 0955-2863
Subject:: biotin; biotinylation; chromatin; esters; genes; histones; humans; interleukin-2; loci; patients; rabbits; terminal repeat sequences; transcriptional activation
Abstract:: ... Holocarboxylase synthetase (HCS) catalyzes the binding of biotin to lysine (K) residues in histones H3 and H4. Histone biotinylation marks are enriched in repressed loci, including retrotransposons. Preliminary studies suggested that K16 in histone H4 is a target for biotinylation by HCS. Here we tested the hypotheses that H4K16bio is a real histone mark in human chromatin and that H4K16bio is ove ...
DOI:: 10.1016/j.jnutbio.2011.10.009
PubMed:: 22192339
PubMed Central:: PMC3314090
: https://doi.org/10.1016/j.jnutbio.2011.10.009

67. Marginal Biotin Deficiency Can Be Induced Experimentally in Humans Using a Cost-Effective Outpatient Design

Author:: Shawna L. Stratton; Cindy L. Henrich; Nell I. Matthews; Anna Bogusiewicz; Amanda M. Dawson; Thomas D. Horvath; Suzanne N. Owen; Gunnar Boysen; Jeffery H. Moran; Donald M. Mock
Source:: Journal of nutrition 2012 v.142 no.1 pp. 22-26
ISSN:: 0022-3166
Subject:: acids; avidin; beverages; binding capacity; biotin; carnitine; diet; egg albumen; excretion; experimental design; humans; lymphocytes; propionyl-CoA carboxylase; women
Abstract:: ... To date, marginal, asymptomatic biotin deficiency has been successfully induced experimentally by the use of labor-intensive inpatient designs requiring rigorous dietary control. We sought to determine if marginal biotin deficiency could be induced in humans in a less expensive outpatient design incorporating a self-selected, mixed general diet. We sought to examine the efficacy of three outpatien ...
DOI:: 10.3945/jn.111.151621
PubMed:: 22157538
PubMed Central:: PMC3237229
: https://doi.org/10.3945/jn.111.151621

68. Milk for humans: Evolving perceptions of an all-time mother science

Author:: Akbar Nikkhah
Source:: Russian agricultural sciences 2012 v.38 no.4 pp. 328-336
ISSN:: 1068-3674
Subject:: agglutinins; alkaline phosphatase; ascorbic acid; asses; beta-carotene; beta-lactoglobulin; biotin; blood pressure; breast milk; calcium; cholesterol; choline; citric acid; dairy cows; dairy goats; digestive system; digestive system diseases; education; enterotoxigenic Escherichia coli; ewe milk; fat globules; fatty liver; folic acid; functional foods; goat milk; heart; horses; human population; humans; hyperlipidemia; hypersensitivity; immunoglobulins; infants; insulin; iron; lactation; magnesium; manganese; medium chain fatty acids; milk; milk consumption; mothers; myo-inositol; neoplasms; niacin; nutrient requirements; orotic acid; ovalbumin; pantothenic acid; peptides; pyridoxine; quality of life; riboflavin; ribonucleases; risk; sodium; thiamin; vitamin B12; whey protein; xanthine oxidase; zinc
Abstract:: ... Milk is the most nourishing natural biofluid and almost an ideal functional food for humans of all ages. The objective of this review article is to delineate global nutritional and health implications of main live-stock dairy products for optimum postmodern life quality. Milk bioactives contribute considerably to meeting nutrient requirements while offering potentials to reduce risks of cancers, t ...
DOI:: 10.3103/S1068367412040143
: https://doi.org/10.3103/S1068367412040143

69. Plant B Vitamin Pathways and their Compartmentation: a Guide for the Perplexed

Author:: Svetlana Gerdes; Claudia Lerma-Ortiz; Océane Frelin; Samuel M. D. Seaver; Christopher S. Henry; Valérie de Crécy-Lagard; Andrew D. Hanson
Source:: Journal of experimental botany 2012 v.63 no.15 pp. 5379-5395
ISSN:: 0022-0957
Subject:: Arabidopsis; biochemical pathways; corn; databases; enzymes; genes; humans; models; nutrition; transporters; vitamin B complex
Abstract:: ... The B vitamins and the cofactors derived from them are essential for life. B vitamin synthesis in plants is consequently as crucial to plants themselves as it is to humans and animals, whose B vitamin nutrition depends largely on plants. The synthesis and salvage pathways for the seven plant B vitamins are now broadly known, but certain enzymes and many transporters have yet to be identified, and ...
DOI:: 10.1093/jxb/ers208
PubMed:: 22915736
: https://doi.org/10.1093/jxb/ers208

70. Robust recombinant FcRn production in mammalian cells enabling oriented immobilization for IgG binding studies

Author:: Giovanni Magistrelli; Pauline Malinge; Nadia Anceriz; Marjorie Desmurs; Sophie Venet; Sébastien Calloud; Bruno Daubeuf; Marie Kosco-Vilbois; Nicolas Fischer
Source:: Journal of immunological methods 2012 v.375 no.1-2 pp. 20-29
ISSN:: 0022-1759
Subject:: albumins; antibodies; biotin; biotinylation; culture media; endothelial cells; enzyme-linked immunosorbent assay; flow cytometry; half life; hamsters; humans; immunoglobulin G; mice; receptors; surface plasmon resonance
Abstract:: ... The MHC class-I related receptor or neonatal Fc receptor (FcRn) protects IgG and albumin from degradation by rescuing them in endothelial cells in a pH dependent fashion and consequently increases their respective half-lives. Monoclonal antibody-based therapies are of increasing interest and characterizing the interaction with FcRn is important for the development of an antibody candidate. In orde ...
DOI:: 10.1016/j.jim.2011.09.002
: https://doi.org/10.1016/j.jim.2011.09.002

71. Sensitive Chemiluminescent Imaging for Chemoselective Analysis of Glycan Expression on Living Cells Using a Multifunctional Nanoprobe

Author:: Han En; Ding Lin; Qian Ruocan; Bao Lei; Ju Huangxian
Source:: Analytical chemistry 2012 v.84 no.3 pp. 1452-1458
ISSN:: 1520-6882
Subject:: DNA; aldehydes; avidin; biotin; chemiluminescence; detection limit; galactose; humans; image analysis; liver neoplasms; models; monitoring; nanogold; neoplasm cells; peroxidase
Abstract:: ... A novel sensitive chemiluminescent (CL) imaging method was developed for in situ monitoring of cell surface glycan expression through chemoselective labeling of carbohydrate motifs and then binding to a multifunctional nanoprobe. The nanoprobe was fabricated by assembling biotin-DNA and a large amount of horseradish peroxidase (HRP) on gold nanoparticles (AuNPs). The chemoselective labeling was pe ...
DOI:: 10.1021/ac203489e
: https://doi.org/10.1021/ac203489e

72. A new strategy based on aptasensor to time-resolved fluorescence assay for adenosine deaminase activity

Author:: Kai Zhang; Minhao Xie; Bin Zhou; Yurong Hua; Zihe Yan; Haiyan Liu; Li-ning Guo; Bing Wu; Biao Huang
Source:: Biosensors & bioelectronics 2013 v.41 pp. 123-128
ISSN:: 0956-5663
Subject:: adenosine; adenosine deaminase; biosensors; biotin; blood serum; detection limit; europium; fluorescence; humans; oligonucleotides
Abstract:: ... Quantitative protein bioanalysis in complex biological fluids presents considerable challenges in biological studies and medical diagnosis. The major obstacles are the background signals from the biological fluids and sensors themselves. Because the europium ion (Eu (III)) has the much longer fluorescence lifetime (1ms) than that of the background (5ns), time-resolved method can be widely used to ...
DOI:: 10.1016/j.bios.2012.07.064
: https://doi.org/10.1016/j.bios.2012.07.064

73. Aptamer modification improves the adenoviral transduction of malignant glioma cells

Author:: Hao Chen; Xiaojing Zheng; BingYan Di; Dongyang Wang; Yaling Zhang; Haibin Xia; Qinwen Mao
Source:: Journal of biotechnology 2013 v.168 pp. 362-366
ISSN:: 0168-1656
Subject:: DNA; biotechnology; biotin; extracellular matrix; gene therapy; humans; image analysis; neoplasm cells; nuclear matrix; oligonucleotides; transgenes; viruses
Abstract:: ... Adenovirus has shown increasing promise in the gene-viral therapy for glioblastoma, a treatment strategy that relies on the delivery of viruses or transgenes into tumor cells. However, targeting of adenovirus to human glioblastoma remains a challenge due to the low expression level of coxsackie and adenovirus receptor (CAR) in glioma cells. Aptamers are small and highly structured single-stranded ...
DOI:: 10.1016/j.jbiotec.2013.10.024
: https://doi.org/10.1016/j.jbiotec.2013.10.024

74. Assembly and mechanism of a group II ECF transporter

Author:: Nathan K. Karpowich; Da-Neng Wang
Source:: Proceedings of the National Academy of Sciences of the United States of America 2013 v.110 no.7 pp. 2534-2539
ISSN:: 0027-8424
Subject:: Listeria; Staphylococcus; Streptococcus thermophilus; Thermotoga maritima; adenosine triphosphate; adenosinetriphosphatase; bacteria; biotin; crystal structure; humans; hydrolysis; pathogens; riboflavin; sequence homology; stoichiometry; thiamin; transporters
Abstract:: ... Energy-coupling factor (ECF) transporters are a recently discovered family of primary active transporters for micronutrients and vitamins, such as biotin, thiamine, and riboflavin. Found exclusively in archaea and bacteria, including the human pathogens Listeria , Streptococcus , and Staphylococcus , ECF transporters may be the only means of vitamin acquisition in these organisms. The subunit comp ...
DOI:: 10.1073/pnas.1217361110
PubMed:: 23359690
PubMed Central:: PMC3574940
: https://doi.org/10.1073/pnas.1217361110

75. Brucella BioR Regulator Defines a Complex Regulatory Mechanism for Bacterial Biotin Metabolism

Author:: Youjun Feng; Jie Xu; Huimin Zhang; Zeliang Chen; Swaminath Srinivas
Source:: Journal of bacteriology 2013 v.195 no.15 pp. 3451-3467
ISSN:: 0021-9193
Subject:: Agrobacterium radiobacter; Brucella melitensis; adenosine triphosphate; beta-galactosidase; biochemical pathways; biosynthesis; biotin; biotin-(acetyl-CoA-carboxylase) ligase; gel electrophoresis; gene overexpression; humans; mutants; operon; plant pathogens; promoter regions; quantitative polymerase chain reaction; transcription (genetics); transcription factors
Abstract:: ... The enzyme cofactor biotin (vitamin H or B7) is an energetically expensive molecule whose de novo biosynthesis requires 20 ATP equivalents. It seems quite likely that diverse mechanisms have evolved to tightly regulate its biosynthesis. Unlike the model regulator BirA, a bifunctional biotin protein ligase with the capability of repressing the biotin biosynthetic pathway, BioR has been recently rep ...
DOI:: 10.1128/JB.00378-13
PubMed:: 23729648
PubMed Central:: PMC3719536
: https://doi.org/10.1128/JB.00378-13

76. Cell-permeable probe for identification and imaging of sialidases

Author:: Charng-Sheng Tsai; Hsin-Yung Yen; Meng-I Lin; Tsung-I Tsai; Shi-Yun Wang; Wen-I Huang; Tsui-Ling Hsu; Yih-Shyun E. Cheng; Jim-Min Fang; Chi-Huey Wong
Source:: Proceedings of the National Academy of Sciences of the United States of America 2013 v.110 no.7 pp. 2466-2471
ISSN:: 0027-8424
Subject:: alkynes; bacteria; biotin; humans; image analysis; influenza; liquid chromatography; mass spectrometry; oseltamivir; sialidase; tyrosine; viruses
Abstract:: ... Alkyne-hinged 3-fluorosialyl fluoride (DFSA) containing an alkyne group was shown to be a mechanism-based target-specific irreversible inhibitor of sialidases. The ester-protected analog DFSA (PDFSA) is a membrane-permeable precursor of DFSA designed to be used in living cells, and it was shown to form covalent adducts with virus, bacteria, and human sialidases. The fluorosialyl–enzyme adduct can ...
DOI:: 10.1073/pnas.1222183110
PubMed:: 23359711
PubMed Central:: PMC3574955
: https://doi.org/10.1073/pnas.1222183110

77. Effect of avidin-like proteins and biotin modification on mesenchymal stem cell adhesion

Author:: Ray C. Schmidt; Kevin E. Healy
Source:: Biomaterials 2013 v.34 no.15 pp. 3758-3762
ISSN:: 0142-9612
Subject:: adhesion; avidin; biotin; biotinylation; blood serum; bovine serum albumin; cell adhesion; cell culture; cost effectiveness; humans; polystyrenes; stem cells; tissue culture
Abstract:: ... The avidin–biotin system is a highly specific reaction that has been used in a wide range of biomedical applications, including surface modification and cell patterning. We systematically examined a number of avidin derivatives as the basis for a simple and cost effective tissue culture polystyrene substrate surface modification for human stem cell culture. Non-specific adhesion between human mese ...
DOI:: 10.1016/j.biomaterials.2013.01.081
PubMed:: 23452388
PubMed Central:: PMC3900499
: https://doi.org/10.1016/j.biomaterials.2013.01.081

78. Enzyme-amplified electronic logic gates based on split/intactaptamers

Author:: Junhua Chen; Lingwen Zeng
Source:: Biosensors & bioelectronics 2013 v.42 pp. 93-99
ISSN:: 0956-5663
Subject:: adenosine monophosphate; adenosine triphosphate; biosensors; biotin; blood serum; cocaine; humans; monitoring; oligonucleotides; theophylline; thrombin
Abstract:: ... A series of enzyme-amplified electronic logic gates (OR, AND, NOR, and NAND) for one-spot simultaneous monitoring of small molecules and proteins has been constructed at the molecular level. This simple but universal system is based on target-induced self-assembly of split aptamer fragments or target-induced conformational changes of intact aptamers. For the OR and AND logic operations, the split ...
DOI:: 10.1016/j.bios.2012.10.030
: https://doi.org/10.1016/j.bios.2012.10.030

79. Identification and assessment of markers of biotin status in healthy adults

Author:: Wei Kay Eng; David Giraud; Vicki L. Schlegel; Dong Wang; Bo Hyun Lee; Janos Zempleni
Source:: British journal of nutrition 2013 v.110 no.2 pp. 321-329
ISSN:: 1475-2662
Subject:: adults; biotin; cell culture; data collection; excretion; gene expression; humans; lymphocytes; men; messenger RNA; metabolism; organic acids and salts; propionyl-CoA carboxylase; transporters; women
Abstract:: ... Human biotin requirements are unknown and the identification of reliable markers of biotin status is necessary to fill this knowledge gap. Here, we used an outpatient feeding protocol to create states of biotin deficiency, sufficiency and supplementation in sixteen healthy men and women. A total of twenty possible markers of biotin status were assessed, including the abundance of biotinylated carb ...
DOI:: 10.1017/S0007114512005065
PubMed:: 23302490
PubMed Central:: PMC4743878
: https://doi.org/10.1017/S0007114512005065

80. Novel Impedimetric Immunosensor for Detection of Pathogenic Bacteria Streptococcus pyogenes in Human Saliva

Author:: Ahmed Asif; Rushworth Jo V.; Wright John D.; Millner Paul A.
Source:: Analytical chemistry 2013 v.85 no.24 pp. 12118-12125
ISSN:: 1520-6882
Subject:: Streptococcus pyogenes; antibodies; bacteria; biotin; cell culture; cross reaction; electrochemistry; electrodes; fluorescence microscopy; gold; human diseases; humans; immunosensors; markets; pathogens; polymerase chain reaction; saliva; scanning electron microscopy
Abstract:: ... Streptococcus pyogenes, also known as group A streptococcus (GAS), is a Gram positive human pathogen responsible for invasive and noninvasive human infections with a high incidence rate. Traditional detection methods involve cell culture and PCR, which are limited by long processing times or the need for high cost equipment. Impedance-based electrochemical immunosensors provide an alternative by w ...
DOI:: 10.1021/ac403253j
: https://doi.org/10.1021/ac403253j

81. Optimization of a human IgG B-cell ELISpot assay for the analysis of vaccine-induced B-cell responses

Author:: Maja Jahnmatz; Gun Kesa; Eva Netterlid; Anne-Marie Buisman; Rigmor Thorstensson; Niklas Ahlborg
Source:: Journal of Immunological Methods 2013 v.391 pp. 50-59
ISSN:: 0022-1759
Subject:: B-lymphocytes; agonists; antigens; avidin; biotin; blood serum; humans; immunoglobulin G; interleukin-10; interleukin-2; monoclonal antibodies; polyclonal antibodies; serology; tetanus; vaccination; vaccines
Abstract:: ... B-cell responses after infection or vaccination are often measured as serum titers of antigen-specific antibodies. Since this does not address the aspect of memory B-cell activity, it may not give a complete picture of the B-cell response. Analysis of memory B cells by ELISpot is therefore an important complement to conventional serology. B-cell ELISpot was developed more than 25years ago and many ...
DOI:: 10.1016/j.jim.2013.02.009
: https://doi.org/10.1016/j.jim.2013.02.009

82. Simultaneous Quantitative Determination of Multiple Mycotoxins in Cereal and Feedstuff Samples by a Suspension Array Immunoassay

Author:: Wang Yuan-Kai; Yan Ya-Xian; Li Shu-Qing; Wang Heng-an; Ji Wen-Hui; Sun Jian-He
Source:: Journal of agricultural and food chemistry 2013 v.61 no.46 pp. 10948-10953
ISSN:: 1520-5118
Subject:: aflatoxin B1; biotin; correlation; deoxynivalenol; detection limit; feeds; fumonisin B1; fungi; humans; immunoassays; mass spectrometry; monoclonal antibodies; toxicity; zearalenone
Abstract:: ... Mycotoxins produced by different species of fungi may coexist in single cereal and feedstuff samples, which could become highly toxic for humans and animals. In order to quantify four mycotoxins (zearalenone, fumonisin B1, deoxynivalenol, and aflatoxin B1) in cereal and feedstuff samples simultaneously, a new suspension array immunoassay was developed. Antimycotoxin monoclonal antibodies were conj ...
DOI:: 10.1021/jf4036029
PubMed:: 24156401
: https://doi.org/10.1021/jf4036029

83. Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfoulingsurface

Author:: Gang Liu; Ruojun Lao; Li Xu; Qin Xu; Lanying Li; Min Zhang; Shiping Song; Chunhai Fan
Source:: Biosensors & bioelectronics 2013 v.42 pp. 516-521
ISSN:: 0956-5663
Subject:: DNA; Hepatitis B virus; absorption; biosensors; biotin; electrochemistry; electrodes; genes; genotyping; gold; humans; mutants; oligonucleotides; peroxidase; polymerase chain reaction; single nucleotide polymorphism
Abstract:: ... A novel electrochemical DNA biosensor for single-nucleotide polymorphism (SNP) analysis was developed. In this work, an oligonucleotide-incorporated nonfouling surface (ONS) was constructed to resist nonspecific absorption. The biosensor was developed using a 16-electrode array for high-throughput SNP analysis. The proposed strategy was primarily based on specific oligonucleotide ligation. Fully m ...
DOI:: 10.1016/j.bios.2012.11.005
: https://doi.org/10.1016/j.bios.2012.11.005

84. Synthesis of Photodegradable Macromers for Conjugation and Release of Bioactive Molecules

Author:: Griffin Donald R.; Schlosser Jessica L.; Lam Sandra F.; Nguyen Thi H.; Maynard Heather D.; Kasko Andrea M.
Source:: Biomacromolecules 2013 v.14 no.4 pp. 1199-1207
ISSN:: 1526-4602
Subject:: alcohols; bioactive properties; biotin; bovine serum albumin; cell adhesion; cell differentiation; chemical elements; enzyme activity; humans; hydrogels; medicine; molecular weight; peptides; phenylalanine; stem cells; therapeutics; transforming growth factor beta 1
Abstract:: ... Hydrogel scaffolds are used in biomedicine to study cell differentiation and tissue evolution, where it is critical to control the delivery of chemical cues both spatially and temporally. While large molecules can be physically entrapped in a hydrogel, moderate molecular weight therapeutics must be tethered to the hydrogel network through a labile linkage to allow controlled release. We synthesize ...
DOI:: 10.1021/bm400169d
PubMed:: 23506440
PubMed Central:: PMC4198304
: https://doi.org/10.1021/bm400169d

85. Three promoters regulate the transcriptional activity of the human holocarboxylase synthetase gene

Author:: Mengna Xia; Sridhar A. Malkaram; Janos Zempleni
Source:: Journal of nutritional biochemistry 2013 v.24 pp. 1963-1969
ISSN:: 0955-2863
Subject:: Drosophila melanogaster; TATA box; binding sites; biotin; biotinylation; colon; culture media; expressed sequence tags; genomic islands; human cell lines; humans; kidneys; loci; longevity; messenger RNA; metabolism; multiprotein complexes; phenotype; plasmids; proteome; rats; reporter genes; stress tolerance; terminal repeat sequences; transcription (genetics); transcription factors
Abstract:: ... Holocarboxylase synthetase (HLCS) is the only protein biotin ligase in the human proteome. HLCS-dependent biotinylation of carboxylases plays crucial roles in macronutrient metabolism. HLCS appears to be an essential part of multiprotein complexes in the chromatin that cause gene repression and contribute toward genome stability. Consistent with these essential functions, HLCS knockdown causes str ...
DOI:: 10.1016/j.jnutbio.2013.06.007
PubMed:: 24075901
PubMed Central:: PMC3805693
: https://doi.org/10.1016/j.jnutbio.2013.06.007

86. Urinary excretion of B-group vitamins reflects the nutritional status of B-group vitamins in rats

Author:: Katsumi Shibata; Chisa Sugita; Mitsue Sano; Tsutomu Fukuwatari
Source:: Journal of nutritional science 2013 v.2 pp. -
ISSN:: 2048-6790
Subject:: 4-pyridoxic acid; animal models; biotin; blood; blood sampling; excretion; folic acid; food intake; humans; normal values; nutritional status; pantothenic acid; pyridoxal phosphate; pyridoxine; rats; riboflavin; thiamin; urine
Abstract:: ... We have reported previously that the urinary excretion of B-group vitamins reflects recent dietary intakes of these vitamins. We also proposed reference values for the urinary levels of B-group vitamins for human subjects, and used these for evaluating human nutritional status. However, the question arises as to whether the urinary excretion of B-group vitamins in animals or human subjects decreas ...
DOI:: 10.1017/jns.2013.3
PubMed:: 25191560
PubMed Central:: PMC4153309
: https://doi.org/10.1017/jns.2013.3

87. A convenient sandwich assay of thrombin in biological media using nanoparticle-enhanced fluorescence polarization

Author:: Qiaoli Yue; Tongfei Shen; Lei Wang; Shuling Xu; Haibo Li; Qingwang Xue; Yuanfu Zhang; Xiaohong Gu; Shuqiu Zhang; Jifeng Liu
Source:: Biosensors & bioelectronics 2014 v.56 pp. 231-236
ISSN:: 0956-5663
Subject:: biosensors; biotin; blood serum; detection limit; fluorescein; fluorescence; humans; nanoparticles; oligonucleotides; silica; streptavidin; thrombin
Abstract:: ... A new aptamer biosensor was presented for the detection of thrombin in this work, which was based on fluorescence polarization (FP) using silica nanoparticles as enhancement probe. The silica nanoparticles covered by streptavidin were tagged with a thrombin aptamer (5'-biotin-GGTTGGTGTGGTTGG-3'), which was bound to the surface of silica nanoparticle through the specific interaction between strepta ...
DOI:: 10.1016/j.bios.2014.01.021
: https://doi.org/10.1016/j.bios.2014.01.021

88. A dual color Southern blot to visualize two genomes or genic regions simultaneously

Author:: Anamaria G. Zavala; Amit S. Kulkarni; Elizabeth A. Fortunato
Source:: Journal of virological methods 2014 v.198 pp. 64-68
ISSN:: 0166-0934
Subject:: Southern blotting; protocols; parasites; Human betaherpesvirus 5; image analysis; humans; mitochondrial DNA; antibodies; fluorescence; nuclear genome; viruses; mitochondria; color; streptavidin; biotin; fibroblasts; bacteria; digoxigenin
Abstract:: ... This report describes the development of a novel dual color Southern protocol to visualize two distinct genomes or genic regions simultaneously on a single Southern blot. The blot is developed with IRDye-conjugated antibody (Ab) and streptavidin that recognize digoxigenin (Dig)- or biotin-labeled probes, respectively and visualized on an infrared imager. This protocol was validated by visualizing ...
DOI:: 10.1016/j.jviromet.2013.12.019
PubMed:: 24389128
PubMed Central:: PMC4010645
: https://doi.org/10.1016/j.jviromet.2013.12.019

89. A universal lateral flow biosensor for proteins and DNAs based on the conformational change of hairpin oligonucleotide and its use for logic gate operations

Author:: Yan Huang; Wei Wen; Dan Du; Xiuhua Zhang; Shengfu Wang; Yuehe Lin
Source:: Biosensors & bioelectronics 2014 v.61 pp. 598-604
ISSN:: 0956-5663
Subject:: ambient temperature; antigens; biosensors; biotin; blood serum; color; diagnostic techniques; humans; nanogold; oligonucleotides; proteins
Abstract:: ... A universal lateral flow biosensor for proteins and DNAs was designed on the base of target-induced conformational changes of hairpin oligonucleotide (HO). CEA (carcinoembryonic antigen) protein and c-DNA were detected both with the naked eye and a strip reader. The scheme of detecting proteins and DNAs were based on the unique molecular recognition properties of HO to the targets to form differen ...
DOI:: 10.1016/j.bios.2014.06.007
: https://doi.org/10.1016/j.bios.2014.06.007

90. Antioxidants successfully reduce ROS production in propionic acidemia fibroblasts

Author:: Lorena Gallego-Villar; Belén Pérez; Magdalena Ugarte; Lourdes R. Desviat; Eva Richard
Source:: Biochemical and biophysical research communications 2014 v.452 pp. 457-461
ISSN:: 0006-291X
Subject:: acidosis; adjuvants; animal models; antioxidants; biotin; fibroblasts; glutathione peroxidase; humans; mitochondria; movement disorders; patients; propionyl-CoA carboxylase; resveratrol; superoxide dismutase; therapeutics
Abstract:: ... Propionic acidemia (PA), caused by a deficiency of the mitochondrial biotin dependent enzyme propionyl-CoA carboxylase (PCC) is one of the most frequent organic acidurias in humans. Most PA patients present in the neonatal period with metabolic acidosis and hyperammonemia, developing different neurological symptoms, movement disorders and cardiac complications. There is strong evidence indicating ...
DOI:: 10.1016/j.bbrc.2014.08.091
: https://doi.org/10.1016/j.bbrc.2014.08.091

91. Biofunctional Surface Patterns Retaining Activity after Exposure to Whole Blood

Author:: Ogaki Ryosuke; Foss Morten
Source:: Langmuir 2014 v.30 no.23 pp. 7014-7023
ISSN:: 1520-5827
Subject:: adsorption; ambient temperature; bioactive properties; biotin; blood; calmodulin; coatings; electrolytes; fluorescence microscopy; humans; mass spectrometry; moieties; nitrilotriacetic acid; polymers; streptavidin; titanium
Abstract:: ... Biofunctional surface patterns capable of resisting nonspecific bioadsorption while retaining bioactivity play crucial roles in the advancement of life science and biomedical technologies. The currently available functional surface coatings suffer from a high level of nonspecific surface adsorption of proteins under biologically challenging conditions, leading to a loss of activity in functional m ...
DOI:: 10.1021/la5007378
: https://doi.org/10.1021/la5007378

92. Cell-free identification of novel N-myristoylated proteins from complementary DNA resources using bioorthogonal myristic acid analogues

Author:: Emi Takamitsu; Kazuki Fukunaga; Yusuke Iio; Koko Moriya; Toshihiko Utsumi
Source:: Analytical biochemistry 2014 v.464 pp. 83-93
ISSN:: 0003-2697
Subject:: azides; biotin; cell-free protein synthesis; chemiluminescence; clones; complementary DNA; copper; cycloaddition reactions; humans; insects; myristic acid; myristoylation; peroxidase; polyacrylamide gel electrophoresis; streptavidin; thermoplastics
Abstract:: ... To establish a non-radioactive, cell-free detection system for protein N-myristoylation, metabolic labeling in a cell-free protein synthesis system using bioorthogonal myristic acid analogues was performed. After Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) with a biotin tag, the tagged proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and blotte ...
DOI:: 10.1016/j.ab.2014.07.006
: https://doi.org/10.1016/j.ab.2014.07.006

93. Electrochemical aptasensor of cellular prion protein based on modified polypyrrole with redox dendrimers

Author:: A. Miodek; G. Castillo; T. Hianik; H. Korri-Youssoufi
Source:: Biosensors & bioelectronics 2014 v.56 pp. 104-111
ISSN:: 0956-5663
Subject:: biosensors; biotin; blood plasma; detection limit; diffusivity; electrochemistry; gold; humans; ions; oligonucleotides; prions; streptavidin
Abstract:: ... This work consists of the development of an electrochemical aptasensor based on polyprrole modified with redox dendrimers, able to detect human cellular prions PrPC with high sensitivity. The gold surface was modified by conductive polypyrrole film coupled to polyamidoamine dendrimers of fourth generation (PAMAM G4) and ferrocenyl group as redox marker. The aptamers were immobilized on the surface ...
DOI:: 10.1016/j.bios.2013.12.051
: https://doi.org/10.1016/j.bios.2013.12.051

94. Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes

Author:: Jiahai Shi; Lenka Kundrat; Novalia Pishesha; Angelina Bilate; Chris Theile; Takeshi Maruyama; Stephanie K. Dougan; Hidde L. Ploegh; Harvey F. Lodish
Source:: Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.28 pp. 10131-10136
ISSN:: 0027-8424
Subject:: antibodies; biotin; blood flow; blood proteins; erythroblasts; humans; mice; plasma membrane; reticulocytes
Abstract:: ... We developed modified RBCs to serve as carriers for systemic delivery of a wide array of payloads. These RBCs contain modified proteins on their plasma membrane, which can be labeled in a sortase-catalyzed reaction under native conditions without inflicting damage to the target membrane or cell. Sortase accommodates a wide range of natural and synthetic payloads that allow modification of RBCs wit ...
DOI:: 10.1073/pnas.1409861111
PubMed:: 24982154
PubMed Central:: PMC4104923
: https://doi.org/10.1073/pnas.1409861111

95. Highly sensitive electrochemical aptasensor for immunoglobulin E detection based on sandwich assay using enzyme-linked aptamer

Author:: Abdollah Salimi; Somayeh Khezrian; Rahman Hallaj; Asaad Vaziry
Source:: Analytical biochemistry 2014 v.466 pp. 89-97
ISSN:: 0003-2697
Subject:: aptasensors; biotin; blood serum; bovine serum albumin; carbon nanotubes; chemical bonding; chitosan; dielectric spectroscopy; electrochemistry; glassy carbon electrode; humans; hydrogen peroxide; immunoglobulin E; ionic liquids; lysozyme; nanocomposites; oligonucleotides; peroxidase
Abstract:: ... Here, we describe the fabrication of an electrochemical immunoglobulin E (IgE) aptasensor using enzyme-linked aptamer in the sandwich assay method and thionine as redox probe. In this protocol, 5′-amine-terminated IgE aptamer and thionine were covalently attached on glassy carbon electrode modified with carbon nanotubes/ionic liquid/chitosan nanocomposite. Furthermore, another IgE aptamer was modi ...
DOI:: 10.1016/j.ab.2014.08.019
: https://doi.org/10.1016/j.ab.2014.08.019

96. Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation

Author:: In-Wook Hwang; Yu Makishima; Tatsuya Kato; Sungjo Park; Andre Terzic; Enoch Y. Park
Source:: Applied microbiology and biotechnology 2014 v.98 no.19 pp. 8201-8209
ISSN:: 0175-7598
Subject:: Bombyx mori; acetyl-CoA carboxylase; antibodies; atomic force microscopy; biotin; biotinylation; catalytic activity; citrates; dephosphorylation; drug therapy; drugs; enzyme activity; humans; image analysis; lipid metabolism; phosphorylation; polyacrylamide gel electrophoresis; polymerization; post-translational modification; silkworms; streptavidin
Abstract:: ... Biotin-dependent human acetyl-CoA carboxylases (ACCs) are integral in homeostatic lipid metabolism. By securing posttranslational biotinylation, ACCs perform coordinated catalytic functions allosterically regulated by phosphorylation/dephosphorylation and citrate. The production of authentic recombinant ACCs is heeded to provide a reliable tool for molecular studies and drug discovery. Here, we ex ...
DOI:: 10.1007/s00253-014-5715-6
PubMed:: 24740690
PubMed Central:: PMC4163189
: https://doi.org/10.1007/s00253-014-5715-6

97. Imperfect centered miRNA binding sites are common and can mediate repression of target mRNAs

Author:: Hilary C Martin; Shivangi Wani; Anita L Steptoe; Keerthana Krishnan; Katia Nones; Ehsan Nourbakhsh; Alexander Vlassov; Sean M Grimmond; Nicole Cloonan
Source:: Genome biology 2014 v.15 no.3 pp. R51
ISSN:: 1474-760X
Subject:: binding sites; biotin; biotinylation; humans; messenger RNA; microRNA; prediction; transcription (genetics); transcriptome; translation (genetics)
Abstract:: ... BACKGROUND: MicroRNAs (miRNAs) bind to mRNAs and target them for translational inhibition or transcriptional degradation. It is thought that most miRNA-mRNA interactions involve the seed region at the 5′ end of the miRNA. The importance of seed sites is supported by experimental evidence, although there is growing interest in interactions mediated by the central region of the miRNA, termed centere ...
DOI:: 10.1186/gb-2014-15-3-r51
PubMed:: 24629056
PubMed Central:: PMC4053950
: https://doi.org/10.1186/gb-2014-15-3-r51

98. Intracellular Routing in Breast Cancer Cells of Streptavidin-Conjugated Trastuzumab Fab Fragments Linked to Biotinylated Doxorubicin-Functionalized Metal Chelating Polymers

Author:: Liu Peng; Cai Zhongli; Kang Jae W.; Boyle Amanda J.; Adams Jarret; Lu Yijie; Mbong Ghislaine Ngo Ndjock; Sidhu Sachdev; Reilly Raymond M.; Winnik Mitchell A.
Source:: Biomacromolecules 2014 v.15 no.3 pp. 715-725
ISSN:: 1526-4602
Subject:: DNA; binding sites; biotin; breast neoplasms; cell fractionation; chemical bonding; chromatin; dissociation; doxorubicin; electrons; erbB-2 receptor; fluorescence; fluorescence microscopy; humans; metal ions; monitoring; neoplasm cells; polyacrylamide; polymerization; radiolabeling; streptavidin; surface plasmon resonance
Abstract:: ... We describe the synthesis of a heterotelechelic metal-chelating polymer (Bi-MCP-Dox), a polyacrylamide with a number average degree of polymerization DPₙ = 50 (PDI = 1.2), with biotin (Bi) and doxorubicin (Dox) as functional chain ends and diethylenetriaminepentaacetic acid (DTPA) pendant groups as the binding sites for metal ions. We compared its behavior in cell-uptake experiments with a similar ...
DOI:: 10.1021/bm401483a
: https://doi.org/10.1021/bm401483a

99. Lysine biotinylation and methionine oxidation in the heat shock protein HSP60 synergize in the elimination of reactive oxygen species in human cell cultures

Author:: Yong Li; Sridhar A. Malkaram; Jie Zhou; Janos Zempleni
Source:: Journal of nutritional biochemistry 2014 v.25 pp. 475-482
ISSN:: 0955-2863
Subject:: biotin; biotinylation; cell culture; cell cycle checkpoints; fibroblasts; heat shock proteins; humans; kidneys; lysine; mass spectrometry; methionine; oxidation; protein structure; proteome; reactive oxygen species
Abstract:: ... Previous studies suggest that the number of proteins containing covalently bound biotin is larger than previously thought. Here, we report the identity of some of these proteins. Using mass spectrometry, we discovered 108 novel biotinylation sites in the human embryonic kidney HEK293 cell proteome; members of the heat shock protein (HSP) superfamily were overrepresented among the novel biotinylate ...
DOI:: 10.1016/j.jnutbio.2013.12.008
PubMed:: 24582286
PubMed Central:: PMC3959574
: https://doi.org/10.1016/j.jnutbio.2013.12.008

100. Novel roles of holocarboxylase synthetase in gene regulation and intermediary metabolism

Author:: Janos Zempleni; Dandan Liu; Daniel Teixeira Camara; Elizabeth L Cordonier
Source:: Nutrition reviews 2014 v.72 no.6 pp. 369-376
ISSN:: 0029-6643
Subject:: biotin; biotinylation; chromatin; fatty acid metabolism; genes; gluconeogenesis; glycolysis; humans; leucine
Abstract:: ... The role of holocarboxylase synthetase (HLCS) in catalyzing the covalent binding of biotin to the five biotin‐dependent carboxylases in humans is well established, as are the essential roles of these carboxylases in the metabolism of fatty acids, the catabolism of leucine, and gluconeogenesis. This review examines recent discoveries regarding the roles of HLCS in assembling a multiprotein gene rep ...
DOI:: 10.1111/nure.12103
PubMed:: 24684412
PubMed Central:: PMC4043915
: https://doi.org/10.1111/nure.12103

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1. Acute Megakaryoblastic Leukemia in One Cat and Two Dogs

2. Expression of Mononuclear Phagocyte Antigens in Histiocytic Sarcoma of Mice

3. Mutational hotspot in the human biotinidase gene causes profound biotinidase deficiency

4. Structure of the human biotinidase gene

5. Studies on the binding site of the galactose-specific agglutinin PA-IL from Pseudomonas aeruginosa

6. Expanding baculovirus surface display: Modification of the native coat protein gp64 of Autographa californica NPV

7. Biotinylation of histones in human cellsâ: Effects of cell proliferation

8. Certain Immune Markers Are Not Good Indicators of Mild to Moderate Biotin Deficiency in Rats

9. Biotinidase catalyzes debiotinylation of histones

10. Indicators of marginal biotin deficiency and repletion in humans: validation of 3-hydroxyisovaleric acid excretion and a leucine challenge

11. Biotin Supplementation Increases Expression of Genes Encoding Interferon-γ, Interleukin-1β, and 3-Methylcrotonyl-CoA Carboxylase, and Decreases Expression of the Gene Encoding Interleukin-4 in Human Peripheral Blood Mononuclear Cells

12. Biotin in microbes, the genes involved in its biosynthesis, its biochemical role and perspectives for biotechnological production

13. Diaminobiotin and Desthiobiotin Have Biotin-Like Activities in Jurkat Cells

14. Marginal Biotin Deficiency Is Teratogenic in ICR Mice

15. Biotinylation of K12 in Histone H4 Decreases in Response to DNA Double-Strand Breaks in Human JAr Choriocarcinoma Cells

16. High-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins in HepG2 Hepatocarcinoma Cells

17. Whole-Genome Analyses of Speciation Events in Pathogenic Brucellae

18. Lymphocyte propionyl-CoA carboxylase and its activation by biotin are sensitive indicators of marginal biotin deficiency in humans

19. An avidin-based assay for histone debiotinylase activity in human cell nuclei

20. K12-biotinylated histone H4 marks heterochromatin in human lymphoblastoma cells

21. Vitamin H-regulated transgene expression in mammalian cells

22. A novel, enigmatic histone modification: biotinylation of histones by holocarboxylase synthetase

23. Aptamer-based endocytosis of a lysosomal enzyme

24. Biotinylation of Histones Represses Transposable Elements in Human and Mouse Cells and Cell Lines and in Drosophila melanogaster

25. Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus

26. Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases

27. gp96 Is a Human Colonocyte Plasma Membrane Binding Protein for Clostridium difficile Toxin A

28. A class of selective antibacterials derived from a protein kinase inhibitor pharmacophore

29. Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food

30. Biotin interaction with human erythrocytes: contact on membrane surface and formation of self-assembled fibrous structures

31. Combination of quantum dot fluorescence with enzyme chemiluminescence for multiplexed detection of lung cancer biomarkers

32. Current understanding of the factors regulating methionine content in vegetative tissues of higher plants

33. DNA microarrays for visual detection of human pathogenic microorganisms based on tyramine signal amplification coupled with gold label silver stain

34. K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts

35. Plasma concentration of 3-hydroxyisovaleryl carnitine is an early and sensitive indicator of marginal biotin deficiency in humans

36. Short Oligonucleotide Tandem Ligation Assay for Genotyping of Single-Nucleotide Polymorphisms in Y Chromosome

37. Studying copper(ii) ion induced interactions of β-amyloid peptides within living cells by gold nanoparticle probes

38. A Biotin Switch-Based Proteomics Approach Identifies 14-3-3ζ as a Target of Sirt1 in the Metabolic Regulation of Caspase-2

39. A method of printing uniform protein lines by using flat PDMS stamps

40. A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure

41. Carbon nano-strings as reporters in lateral flow devices for DNA sensing by hybridization

42. Design, recombinant expression and in vitro maturation of human insulin-like peptide 6 and a biotin-labeled analogue

43. Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum

44. Enzyme-amplified electrochemical biosensor for detection of PML–RARα fusion gene based on hairpin LNA probe

45. Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18

46. Human holocarboxylase synthetase with a start site at methionine-58 is the predominant nuclear variant of this protein and has catalytic activity

47. Identification of holocarboxylase synthetase chromatin binding sites in human mammary cell lines using the DNA adenine methyltransferase identification technology

48. In vivo biotinylated recombinant influenza A virus hemagglutinin for use in subtype-specific serodiagnostic assays

49. Loss of Taste Responds to High-Dose Biotin Treatment

50. Multienzyme-nanoparticles amplification for sensitive virus genotyping in microfluidic microbeads array using Au nanoparticle probes and quantum dots as labels

51. Nanoparticle-based electrochemiluminescence immunosensor with enhanced sensitivity for cardiac troponin I using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles as labels

52. Oxidative stress induced carbonylation in human plasma

53. Proteomic profiling of acrolein adducts in human lung epithelial cells

54. Replication of biotinylated human immunodeficiency viruses

55. Site-Specific Coupling and Sterically Controlled Formation of Multimeric Antibody Fab Fragments with Unnatural Amino Acids

56. Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast

57. Urinary Excretion of 3-Hydroxyisovaleric Acid and 3-Hydroxyisovaleryl Carnitine Increases in Response to a Leucine Challenge in Marginally Biotin-Deficient Humans

58. Urinary Excretion of 3-Hydroxyisovaleryl Carnitine Is an Early and Sensitive Indicator of Marginal Biotin Deficiency in Humans

59. Aluminum Oxide Nanoparticles as Carriers and Adjuvants for Eliciting Antibodies from Non-immunogenic Haptens

60. Avidin Functionalized Maghemite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification

61. Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system

62. Endogenously produced nitric oxide mitigates sensitivity of melanoma cells to cisplatin

63. Engineering of novel tamavidin 2 muteins with lowered isoelectric points and lowered non-specific binding properties

64. Enzyme-Linked Small-Molecule Detection Using Split Aptamer Ligation

65. Identification and quantification of newly synthesized proteins translationally regulated by YB-1 using a novel Click–SILAC approach

66. K16-biotinylated histone H4 is overrepresented in repeat regions and participates in the repression of transcriptionally competent genes in human Jurkat lymphoid cells

67. Marginal Biotin Deficiency Can Be Induced Experimentally in Humans Using a Cost-Effective Outpatient Design

68. Milk for humans: Evolving perceptions of an all-time mother science

69. Plant B Vitamin Pathways and their Compartmentation: a Guide for the Perplexed

70. Robust recombinant FcRn production in mammalian cells enabling oriented immobilization for IgG binding studies

7. Biotinylation of histones in human cellsâ: Effects of cell proliferation