U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term humans Remove constraint Subject term: humans Subject term calcitonin Remove constraint Subject term: calcitonin Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
Start Over

Search

42  Search Results

1 - 42 of 42
Number of results to display per page

Search Results

1. A feline assay using osteoclasts generated in vitro from peripheral blood for screening anti-resorptive agents

Author:
Muzylak, M.; Flanagan, A.M.; Ingham, K.; Gunn, N.; Price, J.; Horton, M.A.
Source:
Research in veterinary science 2002 v.73 no.3 pp. 283-290
ISSN:
0034-5288
Subject:
acid phosphatase; acid tolerance; actin; antigens; blood; bone resorption; calcitonin; cathepsin K; cats; colony-stimulating factors; drugs; humans; integrins; macrophages; osteoclasts; pain; phenotype; rabbits; resorption; screening; transcription factor NF-kappa B
Abstract:
... Musculo-skeletal diseases are a major cause of pain and suffering in cats and several conditions involve increased bone resorption by osteoclasts. However, little is known about the biology of these cells in the cat. In this study we established a method to generate feline osteoclasts from blood mononuclear cells stimulated by macrophage colony stimulating factor (M-CSF) and receptor activator of ...
DOI:
10.1016/S0034-5288(02)00109-1

2. A specific Tween-80-Rhodamine S-MWNTs phosphorescent reagent for the detection of trace calcitonin

Author:
Jia-Ming Liu; Xiao-Mei Huang; Li-Hong Zhang; Zhi-Yong Zheng; Xuan Lin; Xiao-Yang Zhang; Li Jiao; Ma-Lin Cui; Shu-Lian Jiang; Shao-Qin Lin
Source:
Analytica chimica acta 2012 v.744 no. pp. 60-67
ISSN:
0003-2670
Subject:
antibodies; blood serum; calcitonin; carbon nanotubes; cellulose acetate; chemiluminescence immunoassays; humans; infrared spectroscopy; osteoporosis; phosphorescence; polysorbates; product labeling; reaction mechanisms; scanning electron microscopy; temperature
Abstract:
... The present study proposed a simple sensitive and specific immunoassay for the quantification of calcitonin (CT) in human serum with water-soluble multi-walled carbon nanotubes (MWNTs). The COOH group of MWNTs could react with the NH group of rhodamine S (Rhod.S) molecules to form Rhod.S-MWNTs, which could emit room temperature phosphorescence (RTP) on acetate cellulose membrane (ACM) and react wi ...
DOI:
10.1016/j.aca.2012.07.017

3. Adrenomedullin and intermedin gene transcription is increased in leukocytes of patients with chronic heart failure at different stages of the disease

Author:
Cabiati Manuela; Sabatino Laura; Svezia Benedetta; Caruso Raffaele; Verde Alessandro; Caselli Chiara; Prescimone Tommaso; Giannessi Daniela; D.R. Silvia
Source:
Peptides 2014 v.55 pp. 13-16
ISSN:
0196-9781
Subject:
blood sampling; calcitonin; calcitonin gene-related peptide; disease severity; gene expression; heart; heart failure; humans; leukocytes; messenger RNA; pathophysiology; patients; prognosis; quantitative polymerase chain reaction; tissues; transcription (genetics); transcriptomics
Abstract:
... Adrenomedullin (ADM) is a vasodilatory peptide expressed in many tissues. Its levels are elevated in various diseases including chronic heart failure (CHF) and it has been suggested that the up-regulation of ADM in cardiac disease represents a protective mechanism. Similarly, intermedin (IMD), a novel member of the calcitonin/calcitonin gene-related peptide family, is considered a potential endoge ...
DOI:
10.1016/j.peptides.2014.01.028

4. Adrenomedullin production is increased in colorectal adenocarcinomas; its relation to matrix metalloproteinase-9

Author:
Tomomi Hikosaka; Toshihiro Tsuruda; Sayaka Nagata; Kenji Kuwasako; Kazuyo Tsuchiya; Shinri Hoshiko; Haruhiko Inatsu; Kazuo Chijiiwa; Kazuo Kitamura
Source:
Peptides 2011 v.32 no.9 pp. 1825-1831
ISSN:
0196-9781
Subject:
adenocarcinoma; calcitonin; colorectal neoplasms; gelatinase B; genes; humans; messenger RNA; neoplasm cells; pathogenesis; receptors; reverse transcriptase polymerase chain reaction; tissues; vascular endothelial growth factor A
Abstract:
... Adrenomedullin (AM) is highly expressed in various cancer cell lines, suggesting a possible association with cancer growth. In the present study, we examined the expression and/or concentration of AM, its related peptide, adrenomedullin2/intermedin (AM2/IMD) and their receptors in human colorectal cancer and the surrounding normal tissue. In addition, we assessed the correlation between the expres ...
DOI:
10.1016/j.peptides.2011.07.012

5. Adrenomedullin – Current perspective on a peptide hormone with significant therapeutic potential

Author:
Jan-Patrick Fischer; Sylvia Els-Heindl; Annette G. Beck-Sickinger
Source:
Peptides 2020 v.131 pp. 170347
ISSN:
0196-9781
Subject:
amino acids; bioactive properties; calcitonin; calcitonin gene-related peptide; half life; humans; ligands; lymphatic system; peptide hormones; receptors; sequence analysis; therapeutics
Abstract:
... The peptide hormone adrenomedullin (ADM) consists of 52 amino acids and plays a pivotal role in the regulation of many physiological processes, particularly those of the cardiovascular and lymphatic system. Like calcitonin (CT), calcitonin gene-related peptide (CGRP), intermedin (IMD) and amylin (AMY), it belongs to the CT/CGRP family of peptide hormones, which despite their low little sequence id ...
DOI:
10.1016/j.peptides.2020.170347

6. Adrenomedullin: Continuing to explore cardioprotection

Author:
Toshihiro Tsuruda; Johji Kato; Kenji Kuwasako; Kazuo Kitamura
Source:
Peptides 2019 v.111 pp. 47-54
ISSN:
0196-9781
Subject:
aldosterone; amino acids; angiogenesis; apoptosis; biomarkers; calcitonin; cardioprotective effect; clinical trials; cyclic AMP; disulfide bonds; embryogenesis; gene transfer; half life; heart failure; humans; hypertrophy; myocardial infarction; myocardium; oxidative stress; rodents; secretion; therapeutics; vasodilation
Abstract:
... Adrenomedullin (AM), a peptide isolated from an extract of human pheochromocytoma, comprises 52 amino acids with an intramolecular disulfide bond and amidation at the carboxy-terminus. AM is present in various tissues and organs in rodents and humans, including the heart. The peptide concentration increases with cardiac hypertrophy, acute myocardial infarction, and overt heart failure in the plasm ...
DOI:
10.1016/j.peptides.2018.03.012

7. An intra-articular salmon calcitonin-based nanocomplex reduces experimental inflammatory arthritis

Author:
Sinéad M. Ryan; Jason McMorrow; Anita Umerska; Hetal B. Patel; Kristin N. Kornerup; Lidia Tajber; Evelyn P. Murphy; Mauro Perretti; Owen I. Corrigan; David J. Brayden
Source:
Journal of controlled release 2013 v.167 no.2 pp. 120-129
ISSN:
0168-3659
Subject:
anti-inflammatory activity; biomarkers; blood serum; breast neoplasms; calcitonin; chitosan; chondrocytes; gene expression; human cell lines; humans; hyaluronic acid; inflammation; messenger RNA; metalloproteinases; mice; models; monocytes; neoplasm cells; osteoarthritis; pathogenesis; receptors; rheumatoid arthritis; salmon
Abstract:
... Prolonged inappropriate inflammatory responses contribute to the pathogenesis of rheumatoid arthritis (RA) and to aspects of osteoarthritis (OA). The orphan nuclear receptor, NR4A2, is a key regulator and potential biomarker for inflammation and represents a potentially valuable therapeutic target. Both salmon calcitonin (sCT) and hyaluronic acid (HA) attenuated activated mRNA expression of NR4A1, ...
DOI:
10.1016/j.jconrel.2013.01.027

8. Bisphenol A exposure disturbs the bone metabolism: An evolving interest towards an old culprit

Author:
Zar Chi Thent; Gabriele Ruth Anisah Froemming; Suhaila Muid
Source:
Life sciences 2018 v.198 pp. 1-7
ISSN:
0024-3205
Subject:
alkaline phosphatase; apoptosis; bisphenol A; bone resorption; calcitonin; calcium; endocrine-disrupting chemicals; environmental exposure; humans; osteoblasts; osteoclasts; propane; secretion; signal transduction; toxicity
Abstract:
... Bisphenol A (BPA) (2,2,-bis (hydroxyphenyl) propane), a well-known endocrine disruptor (ED), is the exogenous chemical that mimic the natural endogenous hormone like oestrogen. Due to its extensive exposure to humans, BPA is considered to be a major toxicological agent for general population. Environmental exposure of BPA results in adverse health outcomes including bone loss. BPA disturbs the bon ...
DOI:
10.1016/j.lfs.2018.02.013

9. Calcitonin in a protochordate, Ciona intestinalis - the prototype of the vertebrate calcitonin/calcitonin gene-related peptide superfamily

Author:
Sekiguchi, Toshio; Suzuki, Nobuo; Fujiwara, Nobuyuki; Aoyama, Masato; Kawada, Tsuyoshi; Sugase, Kenji; Murata, Yoshiko; Sasayama, Yuichi; Ogasawara, Michio; Satake, Honoo
Source:
FEBS journal 2009 v.276 no.16 pp. 4437-4447
ISSN:
1742-464X
Subject:
Ciona intestinalis; amino acid sequences; calcitonin; calcitonin gene-related peptide; consensus sequence; genes; humans; invertebrates; phylogeny; proline; salmon; tissue distribution
Abstract:
... The calcitonin (CT)/CT gene-related peptides (CGRPs) constitute a large peptide family in vertebrates. However, no CT/CGRP superfamily members have so far been identified in invertebrates, and the evolutionary process leading to the diverse vertebrate CT/CGRP superfamily members remains unclear. In this study, we have identified an authentic invertebrate CT, Ci-CT, in the ascidian Ciona intestinal ...
DOI:
10.1111/j.1742-4658.2009.07151.x
PubMed:
19691140

10. Characterization of a recombinant soluble form of human placental leucine aminopeptidase/oxytocinase expressed in Chinese hamster ovary cells

Author:
Hideko Matsumoto; Tomohiro Rogi; Kyoko Yamashiro; Shiho Kodama; Nobuo Tsuruoka; Akira Hattori; Koji Takio; Shigehiko Mizutani; Masafumi Tsujimoto
Source:
European journal of biochemistry 2000 v.267 no.1 pp. 46-52
ISSN:
0014-2956
Subject:
Chinese hamsters; animal ovaries; blood serum; calcitonin; complementary DNA; cysteine; disulfide bonds; endothelins; humans; leucyl aminopeptidase; molecular weight; oxytocin; peptide hormones; pregnancy; proteins; vasopressin
Abstract:
... Serum levels of human placental leucine aminopeptidase/oxytocinase (P‐LAP) increase with gestation. cDNA cloning of P‐LAP revealed that the enzyme is a type II membrane‐bound protein containing the consensus HEXXH(X)18E motif found in the M1 family of zinc‐metallopeptidase proteins. In this study, a recombinant soluble form of P‐LAP found in maternal serum was expressed in Chinese hamster ovary ce ...
DOI:
10.1046/j.1432-1327.2000.00949.x

11. Characterization of the single transmembrane domain of human receptor activity-modifying protein 3 in adrenomedullin receptor internalization

Author:
Kenji Kuwasako; Kazuo Kitamura; Sayaka Nagata; Naomi Nozaki; Johji Kato
Source:
Biochemical and biophysical research communications 2012 v.420 no.3 pp. 582-587
ISSN:
0006-291X
Subject:
binding capacity; calcitonin; chimerism; flow cytometry; humans; mutants; receptors
Abstract:
... Two receptor activity-modifying proteins (RAMP2 and RAMP3) enable calcitonin receptor-like receptor (CLR) to function as two heterodimeric receptors (CLR/RAMP2 and CLR/RAMP3) for adrenomedullin (AM), a potent cardiovascular protective peptide. Following AM stimulation, both receptors undergo rapid internalization through a clathrin-dependent pathway, after which CLR/RAMP3, but not CLR/RAMP2, can b ...
DOI:
10.1016/j.bbrc.2012.03.037

12. Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: Possible physiological roles of the calcitonin family in osmoregulation

Author:
Nobuo Suzuki; Toshio Sekiguchi; Honoo Satake; Kanoko Kato; Yudai Nishiyama; Hideya Takahashi; Janine A. Danks; T. John Martin; Atsuhiko Hattori; Masaki Nakano; Makiko Kakikawa; Sotoshi Yamada; Maho Ogoshi; Susumu Hyodo; Yoko Yamaguchi; Vishwajit S. Chowdhury; Kazuichi Hayakawa; Hisayuki Funahashi; Tatsuya Sakamoto; Yuichi Sasayama
Source:
Gene 2012 v.499 no.2 pp. 326-331
ISSN:
0378-1119
Subject:
Dasyatis; amino acids; animal ovaries; brain; calcitonin; calcitonin receptors; calcium; cartilage; complementary DNA; euryhaline species; flounder; frogs; gall bladder; humans; kidneys; liver; messenger RNA; metabolism; open reading frames; osmoregulation; pancreas; phylogeny; pituitary gland; seawater; spleen; testes
Abstract:
... In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin rec ...
DOI:
10.1016/j.gene.2012.03.042
PubMed:
22429839

13. Conformationally constrained human calcitonin (hCt) analogues reveal a critical role of sequence 17–21 for the oligomerization state and bioactivity of hCt

Author:
Athanasios Kazantzis; Michaela Waldner; John W. Taylor; Aphrodite Kapurniotu
Source:
European journal of biochemistry 2002 v.269 no.3 pp. 780-791
ISSN:
0014-2956
Subject:
agonists; binding capacity; bone resorption; calcitonin; circular dichroism spectroscopy; humans; osteoporosis; structure-activity relationships; topology
Abstract:
... Calcitonin (Ct) is a 32‐residue peptide hormone that is mainly known for its hypocalcemic effect and the inhibition of bone resorption. Our previous studies have led to potent, side‐chain lactam‐bridged human Ct (hCt) analogues [Kapurniotu, A. Kayed, R., Taylor, J.W. & Voelter W. (1999) Eur. J. Biochem.265, 606–618; Kapurniotu, A. & Taylor, J.W. (1995) J. Med. Chem.38, 836–847]. We have hypothesiz ...
DOI:
10.1046/j.0014-2956.2001.02689.x

14. Cryptorchidism in common eutherian mammals

Author:
Amann, R.P.; Veeramachaneni, D.N.R.
Source:
Reproduction 2007 v.133 no.3 pp. 541-561
ISSN:
1470-1626
Subject:
abdominal cavity; calcitonin; cats; cattle; cauda epididymidis; cryptorchidism; dogs; horses; humans; laboratory animals; ligaments; nerve tissue; pets; rabbits; scrotum; secretion; sheep; swine; terminology; testes; testosterone
Abstract:
... Cryptorchidism is failure of one or both testes to descend into the scrotum. Primary fault lies in the testis. We provide a unifying cross-species interpretation of testis descent and urge the use of precise terminology. After differentiation, a testis is relocated to the scrotum in three sequential phases: abdominal translocation, holding a testis near the internal inguinal ring as the abdominal ...
DOI:
10.1530/REP-06-0272
PubMed:
17379650

15. Detection of Biosynthetic Precursors, Discovery of Glycosylated Forms, and Homeostasis of Calcitonin in Human Cancer Cells

Author:
Cao Feihua; Gamble Allan B.; Onagi Hideki; Howes Joanna; Hennessy James E.; Gu Chen; Morgan Jeremy A. M.; Easton Christopher J.
Source:
Analytical chemistry 2017 v.89 no.13 pp. 6992-6999
ISSN:
1520-6882
Subject:
analytical methods; biosynthesis; calcitonin; carcinogenesis; culture media; enzyme inhibitors; glycosylation; homeostasis; humans; neoplasm cells; neoplasms
Abstract:
... The peptide hormone calcitonin is intimately connected with human cancer development and proliferation. Its biosynthesis is reasoned to proceed via glycine-, α-hydroxyglycine-, glycyllysine-, and glycyllysyllysine-extended precursors; however, as a result of the limitations of current analytical methods, until now, there has been no procedure capable of detecting these individual species in cell o ...
DOI:
10.1021/acs.analchem.7b00457

16. Development and characterization of new rat monoclonal antibodies for procalcitonin

Author:
Krämer, Petra M.; Gouzy, Marie-Françoise; Keß, Melanie; Kleinschmidt, Ulrike; Kremmer, Elisabeth
Source:
Analytical and bioanalytical chemistry 2008 v.392 no.4 pp. 727-736
ISSN:
1618-2642
Subject:
bacterial infections; blood serum; calcitonin; diagnostic techniques; dissociation; enzyme-linked immunosorbent assay; human serum albumin; humans; immunosensors; inflammation; monoclonal antibodies; pH; rats; sepsis (infection); surface plasmon resonance; volunteers
Abstract:
... The development of selective and sensitive biological recognition elements, e.g., antibodies, for the detection of relevant blood markers is a great challenge in the field of biosensors. In this context, five new rat monoclonal antibodies (mAbs) for procalcitonin (PCT), a marker for bacterial infection and sepsis, were developed and characterized. One mAb, PROC1 3G3, was used as capture antibody. ...
DOI:
10.1007/s00216-008-2321-4
PubMed:
18712365

17. Direct Peptide Bioconjugation/PEGylation at Tyrosine with Linear and Branched Polymeric Diazonium Salts

Author:
Jones Mathew W.; Mantovani Giuseppe; Blindauer Claudia A.; Ryan Sinead M.; Wang Xuexuan; Brayden David J.; Haddleton David M.
Source:
Journal of the American Chemical Society 2012 v.134 no.17 pp. 7406-7413
ISSN:
1520-5126
Subject:
animal models; breast neoplasms; calcitonin; calcium; chemical bonding; cyclic AMP; enkephalin; ethylene glycol; humans; in vitro studies; leucine; neoplasm cells; polyethylene glycol; polymerization; rats; receptors; salmon; salts; tyrosine
Abstract:
... Direct polymer conjugation at peptide tyrosine residues is described. In this study Tyr residues of both leucine enkephalin and salmon calcitonin (sCT) were targeted using appropriate diazonium salt-terminated linear monomethoxy poly(ethylene glycol)s (mPEGs) and poly(mPEG) methacrylate prepared by atom transfer radical polymerization. Judicious choice of the reaction conditionspH, stoichiometry, ...
DOI:
10.1021/ja211855q

18. Effects of dietary calcium to available phosphorus ratios on bone metabolism and osteoclast activity of the OPG /RANK/RANKL signalling pathway in piglets

Author:
Lei Zhao; Muyang Li; Hui Sun
Source:
Journal of animal physiology and animal nutrition 2019 v.103 no.4 pp. 1224-1232
ISSN:
0931-2439
Subject:
acid phosphatase; alkaline phosphatase; blood; bone metabolism; bones; calcitonin; calcium; cholecalciferol; dietary minerals; growth performance; hormone secretion; humans; hydroxyapatite; insulin-like growth factor I; interleukin-1; interleukin-6; ligands; osteocalcin; osteoclasts; osteoporosis; parathyroid hormone; phosphorus; piglets; signal transduction; swine feeding; teeth; transcription factor NF-kappa B; transforming growth factor beta
Abstract:
... Hydroxyapatite, a mineral form of calcium (Ca) and phosphorus (P) that gives bones their rigidity, is the major and essential component of bones and teeth in the human and animal body. A suitable ratio of Ca and P is vital for bone growth. The aim of this study was to explore the effects of dietary calcium to available phosphorus ratios (Ca/AP) on bone metabolism and osteoclast activity of the ost ...
DOI:
10.1111/jpn.13115

19. Elevated Lifetime Lead Exposure Impedes Osteoclast Activity and Produces an Increase in Bone Mass in Adolescent Mice

Author:
Eric E. Beier; Jonathan D. Holz; Tzong-Jen Sheu; J. Edward Puzas
Source:
Toxicological sciences 2016 v.149 no.2 pp. 277-288
ISSN:
1096-0929
Subject:
adolescents; bone density; bone formation; calcitonin; dose response; estrogens; femur; heavy metals; humans; lead; osteoclasts; resorption; toxicology
Abstract:
... The heavy metal lead (Pb) has a deleterious effect on skeletal health. Because bone mass is maintained through a balance of bone formation and resorption, it is important to understand the effect of Pb levels on osteoblastic and osteoclastic activity. Pb exposure is associated with low bone mass in animal models and human populations; however, the correlation between Pb dosing and corresponding bo ...
DOI:
10.1093/toxsci/kfv234

20. Expression of Human Calcitonin by Microencapsulated Recombinant Myoblasts

Author:
Wang, Yeming; Zeng, Bingfang; Li, Xiaolin
Source:
Biotechnology letters 2006 v.28 no.18 pp. 1453-1458
ISSN:
0141-5492
Subject:
Western blotting; biopharmaceuticals; calcitonin; cell engineering; clones; complementary DNA; culture media; enzyme-linked immunosorbent assay; gene therapy; genes; hosts; humans; immunohistochemistry; mice; microencapsulation; myoblasts; osteoporosis; secretion
Abstract:
... A novel approach to treat post-menopausal osteoporosis is proposed by engineering cell lines for the secretion of human calcitonin (hCT) and which would then be suitable for implantation in different allogeneic hosts. Mouse myoblast were transfected with the cDNA for hCT using the liposome-based gene delivery technique and clones secreting of human calcitonin were isolated. Human calcitonin expres ...
DOI:
10.1007/s10529-006-9115-6
PubMed:
16823598

21. Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites

Author:
Hengyi Sun; Xiaonan Zang; Yuantao Liu; Xiaofei Cao; Fei Wu; Xiaoyun Huang; Minjie Jiang; Xuecheng Zhang
Source:
Applied microbiology and biotechnology 2015 v.99 no.23 pp. 10097-10106
ISSN:
0175-7598
Subject:
DNA fragmentation; Saccharomyces cerevisiae; Southern blotting; Western blotting; acetates; antibiotics; blood serum; bone metabolism; calcitonin; calcium; chimerism; digestion; enzyme-linked immunosorbent assay; freeze drying; genes; genetic markers; homeostasis; homologous recombination; humans; lithium; mice; mutants; nucleotide sequences; oral administration; osteoporosis; phosphoglycerate kinase; phosphorus; plasmids; proteins; restriction endonucleases; ribosomal DNA; salmon; yeasts
Abstract:
... Calcitonin participates in controlling homeostasis of calcium and phosphorus and plays an important role in bone metabolism. The aim of this study was to endow an industrial strain of Saccharomyces cerevisiae with the ability to express chimeric human/salmon calcitonin (hsCT) without the use of antibiotics. To do so, a homologous recombination plasmid pUC18-rDNA2-ura3-P ₚgₖ -5hsCT-rDNA1 was constr ...
DOI:
10.1007/s00253-015-6834-4

22. Identification of a novel ‘aggregation-prone’/‘amyloidogenic determinant’ peptide in the sequence of the highly amyloidogenic human calcitonin

Author:
Vassiliki A. Iconomidou; Aristeidis Leontis; Andreas Hoenger; Stavros J. Hamodrakas
Source:
FEBS letters 2013 v.587 no.6 pp. 569-574
ISSN:
0014-5793
Subject:
Fourier transform infrared spectroscopy; X-radiation; alanine; amyloid; bones; calcitonin; calcium; carcinoma; cysteine; humans; light microscopy; metabolism; polarized light; polypeptides; transmission electron microscopy
Abstract:
... Calcitonin is a 32-residue polypeptide hormone, which takes part in calcium metabolism in bones. It may form amyloid fibrils. Amyloid fibrils are related with serious diseases known as amyloidoses. The amyloid form of calcitonin takes part in medullary thyroid carcinoma. A novel hexapeptide (6TCMLGT11) of human calcitonin was predicted as a possible ‘aggregation-prone’ peptide, which may play a ro ...
DOI:
10.1016/j.febslet.2013.01.031

23. Imprinted ZnO nanostructure-based electrochemical sensing of calcitonin: A clinical marker for medullary thyroid carcinoma

Author:
Santanu Patra; Ekta Roy; Rashmi Madhuri; Prashant K. Sharma
Source:
Analytica chimica acta 2015 v.853 pp. 271-284
ISSN:
0003-2670
Subject:
blood serum; calcitonin; carcinoma; clinical trials; cost effectiveness; cross reaction; detection limit; electrochemistry; electrodes; humans; molecular imprinting; nanomaterials; polymerization; polymers; scanning electron microscopy; statistical analysis; tyrosine; zinc oxide
Abstract:
... The present work describes an exciting method for the selective and sensitive determination of calcitonin in human blood serum samples. Adopting the surface molecular imprinting technique, a calcitonin-imprinted polymer was prepared on the surface of the zinc oxide nanostructure. Firstly, a biocompatible tyrosine derivative as a monomer was grafted onto the surface of zinc oxide nanostructure foll ...
DOI:
10.1016/j.aca.2014.10.030

24. Inhibiting Human Calcitonin Fibril Formation with Its Most Relevant Aggregation-Resistant Analog

Author:
Yi-Ting Chen; Kai-Wei Hu; Bo-Jie Huang; Chian-Hui Lai; Ling-Hsien Tu
Source:
Journal of physical chemistry 2019 v.123 no.48 pp. 10171-10180
ISSN:
1520-5207
Subject:
adverse effects; amyloid; blood; bones; calcitonin; calcium; humans; immune response; kidneys; mutation; osteoclasts; osteoporosis; phosphates; polypeptides; resorption; salmon; therapeutics; thyroid gland
Abstract:
... The most common obstacles to the development of therapeutic polypeptides are peptide stability and aggregation. Human calcitonin (hCT) is a 32-residue hormone polypeptide secreted from the C-cells of the thyroid gland and is responsible for calcium and phosphate regulation in the blood. hCT reduces calcium levels by inhibiting the activity of osteoclasts, which are bone cells that are mainly respo ...
DOI:
10.1021/acs.jpcb.9b08514

25. Inhibitory effects of two G protein-coupled receptor kinases on the cell surface expression and signaling of the human adrenomedullin receptor

Author:
Kenji Kuwasako; Toshio Sekiguchi; Sayaka Nagata; Danfeng Jiang; Hidetaka Hayashi; Manabu Murakami; Yuichi Hattori; Kazuo Kitamura; Johji Kato
Source:
Biochemical and biophysical research communications 2016 v.470 pp. 894-899
ISSN:
0006-291X
Subject:
calcitonin; calcitonin receptors; cyclic AMP; flow cytometry; gene overexpression; humans; iodine; mutants; phosphotransferases (kinases)
Abstract:
... Receptor activity-modifying protein 2 (RAMP2) enables the calcitonin receptor-like receptor (CLR, a family B GPCR) to form the type 1 adrenomedullin receptor (AM1 receptor). Here, we investigated the effects of the five non-visual GPCR kinases (GRKs 2 through 6) on the cell surface expression of the human (h)AM1 receptor by cotransfecting each of these GRKs into HEK-293 cells that stably expressed ...
DOI:
10.1016/j.bbrc.2016.01.138

26. Knockdown of a G protein-coupled receptor through efficient peptide-mediated siRNA delivery

Author:
Jan Hoyer; Ines Neundorf
Source:
Journal of controlled release 2012 v.161 no.3 pp. 826-834
ISSN:
0168-3659
Subject:
G-protein coupled receptors; calcitonin; cytotoxicity; fatty acids; gene expression; genes; humans; messenger RNA; models; oligonucleotides; osteoporosis; plasma membrane; protein synthesis; small interfering RNA; therapeutics
Abstract:
... In recent years, therapeutic applications of siRNAs have come into the focus of pharmaceutical research owing to their potential to specifically regulate gene expression. However, oligonucleotides have to overcome a series of extracellular and intracellular barriers which is why delivery systems helping to overcome these barriers are desperately needed. A promising approach to transport nucleic ac ...
DOI:
10.1016/j.jconrel.2012.05.017

27. Management of acute renal failure with delayed hypercalcemia secondary to sarcocystis neurona–induced myositis and rhabdomyolysis in a california sea lion (zalophus californianus)

Author:
Alexander Amy B.; Hanley Christopher S.; Duncan Mary C.; Ulmer Kyle; Padilla Luis R.
Source:
Journal of zoo and wildlife medicine 2015 v.46 no.3 pp. 652-656
ISSN:
1042-7260
Subject:
Sarcocystis; Zalophus californianus; antibiotics; antiemetics; calcitonin; calcium; diuretics; fluid therapy; humans; hypercalcemia; myositis; renal failure; rhabdomyolysis; risk
Abstract:
... A 3-yr-old captive-born California sea lion (Zalophus californianus) developed Sarcocystis neurona–induced myositis and rhabdomyolysis that led to acute renal failure. The sea lion was successfully managed with fluid therapy, antiprotozoals, antibiotics, anti-inflammatories, antiemetics, gastroprotectants, and diuretics, but developed severe delayed hypercalcemia, a syndrome identified in humans a ...
DOI:
10.1638/2015-0075.1

28. PK/PD modelling of comb-shaped PEGylated salmon calcitonin conjugates of differing molecular weights

Author:
Sinéad M. Ryan; Jesús M. Frías; Xuexuan Wang; Claire T. Sayers; David M. Haddleton; David J. Brayden
Source:
Journal of controlled release 2011 v.149 no.2 pp. 126-132
ISSN:
0168-3659
Subject:
blood serum; calcitonin; calcium; chymotrypsin; elastase; enzyme stability; half life; high performance liquid chromatography; humans; intravenous injection; liver; mathematical models; molecular weight; polymers; rats; salmon; trypsin
Abstract:
... Salmon calcitonin (sCT) was conjugated via cysteine-1 to novel comb-shaped end-functionalised (poly(PEG) methyl ether methacrylate) (sCT-P) polymers, to yield conjugates of total molecular weights (MW) inclusive of sCT: 6.5, 9.5, 23 and 40kDa. The conjugates were characterised by HPLC and their in vitro and in vivo bioactivity was measured by cAMP assay on human T47D cells and following intravenou ...
DOI:
10.1016/j.jconrel.2010.10.004

29. Procalcitonin Detection in Veterinary Species: Investigation of Commercial ELISA Kits

Author:
Federica Battaglia; Valentina Meucci; Rosalba Tognetti; Francesca Bonelli; Micaela Sgorbini; George Lubas; Carlo Pretti; Luigi Intorre
Source:
Animals 2020 v.10 no.9 pp. -
ISSN:
2076-2615
Subject:
calcitonin; cattle; detection limit; dogs; enzyme-linked immunosorbent assay; horses; humans; medicine; veterinary medicine
Abstract:
... In human medicine, procalcitonin (PCT), the precursor of calcitonin, is used for the rapid identification of the origin and severity of sepsis. In veterinary medicine, PCT has been studied in horses, cattle, and dogs, but the use of PCT in diagnostic and/or prognostic settings is not possible because of the lack of validated assays to obtain reference ranges. The aim of the present study was the i ...
DOI:
10.3390/ani10091511

30. Protective effects of calcitonin on IL-1 stimulated chondrocytes by regulating MMPs/TIMP-1 ratio via suppression of p50-NF-κB pathway

Author:
Xiaodong Bai; Ai Guo; Yadong Li
Source:
Bioscience, biotechnology, and biochemistry 2019 v.83 no.4 pp. 598-604
ISSN:
1347-6947
Subject:
biotechnology; calcitonin; cell viability; chondrocytes; humans; metalloproteinases; models; osteoarthritis; protective effect
Abstract:
... The aim of this study was to investigate the effects and underlying mechanisms of calcitonin (CT) on interleukin 1 beta (IL-1β) stimulated human chondrocytes. IL-1β (5 ng/mL) was added into chondrocytes to establish osteoarthritis (OA) model in vitro. Different concentrations of CT (0.1, 0.5, 1, 5, 10 and 50 nM) were used for treating IL-1β stimulated chondrocytes. Cell viability of chondrocytes w ...
DOI:
10.1080/09168451.2018.1559718

31. Review of hypocalcemia in septic patients

Author:
Holowaychuk, Marie K.; Martin, Linda G.
Source:
Journal of veterinary emergency and critical care 2007 v.17 no.4 pp. 348-358
ISSN:
1479-3261
Subject:
calcitonin; calcium; disease severity; enterocolitis; etiology; horses; humans; hypocalcemia; ionization; models; mortality; patients; prospective studies
Abstract:
... To review the occurrence and etiologies of hypocalcemia in septic human and veterinary patients. A thorough search was conducted using CAB abstracts and MEDLINE and the keywords hypocalcemia, ionized calcium, sepsis, and procalcitonin (proCT). Ionized hypocalcemia (iHCa) is a common finding in septic human patients. The cause is unknown but is likely multifactorial. Low ionized calcium (iCa²⁺) con ...
DOI:
10.1111/j.1476-4431.2007.00246.x

32. Screening of potential GCMS derived antimigraine compound from the leaves of Abrus precatorius Linn to target “calcitonin gene related peptide” receptor using in silico analysis

Author:
Parthasarathy V.; Ajay Kumar T.V
Source:
Food science and human wellness 2019 v.8 no.1 pp. 34-39
ISSN:
2213-4530
Subject:
Abrus precatorius; antagonists; calcitonin; calcitonin gene-related peptide; computer simulation; computer software; drugs; food science; headache; humans; leaves; ligands; liver; migraine; peptide receptors; prediction; screening; toxicity
Abstract:
... Calcitonin receptor-like receptor (CRLR) is a human protein, that produces a calcitonin gene-related peptide receptor (CGRP) when associates with human receptor activity-modifying protein-1 (HRAMP1). CGRP is believed to be involved in triggering of migraine. Many strategies were employed to design antimigraine drug using various CGRP antagonist/ligands but most of them have failed due to their ina ...
DOI:
10.1016/j.fshw.2019.01.001

33. Spatial expression of components of a calcitonin receptor-like receptor (CRL) signalling system (CRL, calcitonin gene-related peptide, adrenomedullin, adrenomedullin-2/intermedin) in mouse and human heart valves

Author:
Uwe Pfeil; Subhashini Bharathala; Ghulam Murtaza; Petra Mermer; Tamara Papadakis; Andreas Boening; Wolfgang Kummer
Source:
Cell and tissue research 2016 v.366 no.3 pp. 587-599
ISSN:
0302-766X
Subject:
blood flow; calcitonin; calcitonin gene-related peptide; endothelial cells; endothelium; extracellular matrix; fluorescent antibody technique; heart valves; histochemistry; humans; ligands; messenger RNA; mice; myocytes; nerve fibers; nitric oxide synthase; proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; smooth muscle
Abstract:
... Heart valves are highly organized structures determining the direction of blood flow through the heart. Smooth muscle cells within the valve are thought to play an active role during the heart cycle, rather than being just passive flaps. The mature heart valve is composed of extracellular matrix (ECM), various differentiations of valvular interstitial cells (VIC), smooth muscle cells and overlying ...
DOI:
10.1007/s00441-016-2473-z

34. Stability and Structure of Protein–Lipoamino Acid Colloidal Particles: Toward Nasal Delivery of Pharmaceutically Active Proteins

Author:
Bijani Christian; Arnarez Clément; Brasselet Sabrina; Degert Corinne; Broussaud Olivier; Elezgaray Juan; Dufourc Erick J.
Source:
Langmuir 2012 v.28 no.13 pp. 5783-5794
ISSN:
1520-5827
Subject:
acids; anemia; calcitonin; calcium; children; circular dichroism spectroscopy; colloids; droplet size; erythropoietin; humans; hydrophobicity; intravenous injection; light scattering; micelles; molecular dynamics; nose; proline; protein structure; salmon; somatotropin
Abstract:
... To circumvent the painful intravenous injection of proteins in the treatment of children with growth deficiency, anemia, and calcium insufficiency, we investigated the stability and structure of protein–lipoamino acid complexes that could be nasally sprayed. Preparations that ensure a colloidal and structural stability of recombinant human growth hormone (rhGH), recombinant human erythropoietin (r ...
DOI:
10.1021/la300222v

35. Structure–function analysis of amino acid 74 of human RAMP1 and RAMP3 and its role in peptide interactions with adrenomedullin and calcitonin gene-related peptide receptors

Author:
Tao Qi; Kien Ly; David R. Poyner; George Christopoulos; Patrick M. Sexton; Debbie L. Hay
Source:
Peptides 2011 v.32 no.5 pp. 1060-1067
ISSN:
0196-9781
Subject:
amino acid composition; amino acids; calcitonin; calcitonin gene-related peptide; calcitonin receptors; cyclic AMP; enzyme-linked immunosorbent assay; humans; mutants; pharmacology
Abstract:
... The receptors for calcitonin gene-related peptide (CGRP) and adrenomedullin (AM) are complexes of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying proteins (RAMP). The CGRP receptor is a CLR/RAMP1 pairing whereas CLR/RAMP2 and CLR/RAMP3 constitute two subtypes of AM receptor: AM₁ and AM₂, respectively. Previous studies identified Glu74 in RAMP3 to be important for AM bin ...
DOI:
10.1016/j.peptides.2011.03.004

36. Structure–function analysis of helix 8 of human calcitonin receptor-like receptor within the adrenomedullin 1 receptor

Author:
Kenji Kuwasako; Kazuo Kitamura; Sayaka Nagata; Tomomi Hikosaka; Johji Kato
Source:
Peptides 2011 v.32 no.1 pp. 144-149
ISSN:
0196-9781
Subject:
G-protein coupled receptors; calcitonin; cyclic AMP; flow cytometry; humans; inhibitory concentration 50; mutants; mutation; structure-activity relationships
Abstract:
... Adrenomedullin 1 (AM₁) receptor is a heterodimer composed of calcitonin receptor-like receptor (CLR) – a family B G protein-coupled receptor (GPCR) – and receptor activity-modifying protein 2 (RAMP2). Both family A and family B GPCRs possess an eighth helix (helix 8) in the proximal portion of their C-terminal tails; however, little is known about the function of helix 8 in family B GPCRs. We ther ...
DOI:
10.1016/j.peptides.2010.10.005

37. Synthesis, characterization and evaluation of bone targeting salmon calcitonin analogs in normal and osteoporotic rats

Author:
Krishna Hari Bhandari; Madhuri Newa; Jillian Chapman; Michael R. Doschak
Source:
Journal of controlled release 2012 v.158 no.1 pp. 44-52
ISSN:
0168-3659
Subject:
animal models; antibodies; bone density; breast neoplasms; calcitonin; calcium; chemical bonding; cytotoxicity; drugs; enzyme-linked immunosorbent assay; humans; hydroxyapatite; micro-computed tomography; neoplasm cells; osteoporosis; ovariectomy; pharmacokinetics; placebos; rats; salmon
Abstract:
... In order to assess the therapeutic efficacy of an antiresorptive drug with imparted bone targeting potential using bisphosphonate (BP) conjugation and an improved pharmacokinetic profile using PEGylation, we synthesized, characterized and evaluated in vivo efficacy of bone-targeting PEGylated salmon calcitonin (sCT) analog (sCT-PEG-BP). sCT-PEG-BP was compared with non-PEGylated bone targeting sCT ...
DOI:
10.1016/j.jconrel.2011.09.096

38. The action of CGRP and SP on cultured skin fibroblasts

Author:
Bernardo Hochman; Vanina M. Tucci-Viegas; Paola K. P. Monteiro; Jerônimo P. França; Silvana Gaiba; Lydia M. Ferreira
Source:
Central European journal of biology 2014 v.9 no.7 pp. 717-726
ISSN:
1895-104X
Subject:
calcitonin; databases; fibroblasts; humans; substance P; tissue repair; vasoactive intestinal peptide
Abstract:
... BACKGROUND/PURPOSE: Calcitonin gene-related peptide (CGRP) is the most abundant neuropeptide in the skin, followed by substance P (SP), vasoactive intestinal peptide (VIP), and other neuropeptides in smaller amounts. The proliferative effect of neuropeptides on fibroblasts may affect wound healing and may be associated with hyperproliferative skin and mesenchymal disorders. Understanding the neuro ...
DOI:
10.2478/s11535-014-0301-6

39. The role of the ERK1/2 pathway as an alternative to the aging-diminished cyclic AMP pathway in calcitonin-mediated chondrogenesis in human nucleus pulposus

Author:
Wei-Hong Chen; Rong Zeng; Wen-Cheng Lo; Szu-Yu Tina Chen; Tung-Yuan Lai; David F. Williams; Win-Ping Deng
Source:
Biomaterials 2012 v.33 no.33 pp. 8256-8264
ISSN:
0142-9612
Subject:
calcitonin; calcitonin receptors; chondrogenesis; cyclic AMP; humans; phenotype; signal transduction; therapeutics
Abstract:
... Human disc degeneration initiated by aging in the central nucleus pulposus (hNP) is an irreversible process and the recovery has become seriously emerging. In this study, the related mechanisms of calcitonin on the regeneration of hNP and the effects of calcitonin on the age-related alterations were examined. The harvested hNP population was designated as YhNP (from young donor, age <50) and OhNP ...
DOI:
10.1016/j.biomaterials.2012.06.088

40. Tyrosine 12 of human calcitonin modulates its amyloid formation, membrane binding, and bioactivity

Author:
I-Ching Hsieh; Ting-Wei Chen; Ya-Ping Chuang; Yun-Ju Lai; Ling-Hsien Tu
Source:
Biochimie 2022 v.197 pp. 121-129
ISSN:
0300-9084
Subject:
amyloid; bioactive properties; bioavailability; blood; calcitonin; calcium; computer software; drugs; humans; osteoporosis; prediction; therapeutics; tyrosine
Abstract:
... Irreversible aggregation greatly limits the bioavailability and therapeutic activity of peptide-based drugs, so preventing protein or peptide aggregation is a common issue in drug formulation. Human calcitonin (hCT), a peptide hormone secreted by thyroidal parafollicular cells, can regulate blood calcium levels and maintain bone structure. Hence, it can be used as a treatment for metabolic bone di ...
DOI:
10.1016/j.biochi.2022.02.007

41. β-arrestins negatively control human adrenomedullin type 1-receptor internalization

Author:
Kenji Kuwasako; Kazuo Kitamura; Sayaka Nagata; Toshio Sekiguchi; Jiang Danfeng; Manabu Murakami; Yuichi Hattori; Johji Kato
Source:
Biochemical and biophysical research communications 2017 pp. -
ISSN:
0006-291X
Subject:
agonists; beta-2 adrenergic receptors; calcitonin; chimerism; clinical trials; cyclic AMP; flow cytometry; heart failure; humans; iodine; pain; protective effect
Abstract:
... Adrenomedullin (AM) is a potent hypotensive peptide that exerts a powerful variety of protective effects against multiorgan damage through the AM type 1 receptor (AM1 receptor), which consists of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 2 (RAMP2). Two β-arrestin (β-arr) isoforms, β-arr-1 and β-arr-2, play a central role in the agonist-induced internalizat ...
DOI:
10.1016/j.bbrc.2017.04.083

42. “Gold-plated” IRMOF-3 and sea cucumber-like Pd@PtRh SNRs based sandwich-type immunosensor for dual-mode detection of PCT

Author:
Hui Dong; Linlin Cao; Huan Zhao; Shanghua Liu; Qing Liu; Ping Wang; Zhen Xu; Shujun Wang; Yueyuan Li; Peiqing Zhao; Yueyun Li
Source:
Biosensors & bioelectronics 2020 v.170 pp. 112667
ISSN:
0956-5663
Subject:
biomarkers; blood serum; calcitonin; catalytic activity; durability; electrochemistry; humans; immunosensors; nanocomposites; nanogold; nanorods; voltammetry
Abstract:
... Electrochemical immunoassays are often used in the detection of biomarkers, and their sensitivity depends on the nature of the substrate and the catalytic activity of the signal amplification platform. In this work, a novel sandwich-type signal amplification strategy with a “gold-plated” organometallic frame (Au/IRMOF-3) as the substrate and the sea cucumber-like Pd@PtRh trimetallic nanomaterial ( ...
DOI:
10.1016/j.bios.2020.112667