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1. Meso-molding three-dimensionally ordered macroporous alumina: A new platform to immobilize enzymes with high performance

Author:
He, Ying,   et al.   ; Zhou, Liya; Luo, Xucong; Li, Jiaojiao; Ma, Li; Jiang, Yanjun; Yin, Luyan; Gao, Liya;     Show all 8 Authors
Source:
Biochemical engineering journal 2019 v.146 pp. 60-68
ISSN:
1369-703X
Subject:
Pseudozyma antarctica; acrylamides; aluminum; aluminum oxide; carboxylic ester hydrolases; composite polymers; confocal laser scanning microscopy; crystals; glutaraldehyde; immobilized enzymes; macropores; nitrile hydratase; palmitic acid; penicillin amidase; polystyrenes; porous media; thermal stability
Abstract:
... The meso-molding three-dimensionally ordered macroporous alumina (3DOM/m-Al) was synthesized via a dual-templating approach (polystyrene (PS) colloidal crystals as hard template and triblock copolymer P123 as soft template) with hydrous aluminum isopropoxide as precursor. The obtained 3DOM/m-Al was characterized, and the results showed that 3DOM/m-Al had ordered macropores (ca. 300 nm in diameter) ...
DOI:
10.1016/j.bej.2019.03.002

2. Virus-like organosilica nanoparticles for lipase immobilization: Characterization and biocatalytic applications

Author:
He, Ying,   et al.   ; Jiang, Yanjun; Liu, Huan; Wang, Lihui; Zhou, Liya; Huang, Zhihong; Ma, Li; Shi, Lujing; Gao, Jing;     Show all 9 Authors
Source:
Biochemical engineering journal 2019 v.144 pp. 125-134
ISSN:
1369-703X
Subject:
Pseudozyma antarctica; alcohols; carboxylic ester hydrolases; esterification; glutaraldehyde; hydrolysis; hydrophobic bonding; hydrophobicity; immobilized enzymes; levulinic acid; nanoparticles; nanotubes; pH; porous media; response surface methodology; silica; solvents; storage quality; temperature; thermal stability
Abstract:
... Hydrophobic virus-like organosilica nanoparticles (VOSNs) with a spherical core formed by mesoporous organosilica surrounded by epitaxial perpendicular nanotubes were successfully synthesized for the first time. These hydrophobic VOSNs were used for the immobilization of the lipase B from Candida antarctica (CALB) via covalent binding method, where glutaraldehyde was used as a spacer to form coval ...
DOI:
10.1016/j.bej.2019.01.022

3. Enzyme@silica hybrid nanoflowers shielding in polydopamine layer for the improvement of enzyme stability

Author:
He, Ying,   et al.   ; Jiang, Yanjun; Zhai, Junqing; Zhou, Liya; Ma, Li; Gao, Jing;     Show all 6 Authors
Source:
Biochemical engineering journal 2018 v.132 pp. 196-205
ISSN:
1369-703X
Subject:
adsorption; coatings; dopamine; emulsions; enzyme stability; immobilized enzymes; nanoflowers; nanoparticles; pH; penicillin amidase; polymerization; silica; storage quality; temperature
Abstract:
... In this study, a novel kind of flower-like monodisperse silica nanoparticles (silica nanoflowers) were successfully synthesized based on the microemulsion phase composed of water-surfactant-oil ternary systems. The obtained silica nanoflowers were used to immobilize penicillin G acylase (PGA). The maximum adsorption capacity of immobilized PGA (PGA@nanoflowers) was 207.3 mg/support and the specifi ...
DOI:
10.1016/j.bej.2018.01.028

4. Formation of cross-linked nitrile hydratase aggregates in the pores of tannic-acid-templated magnetic mesoporous silica: Characterization and catalytic application

Author:
He, Ying,   et al.   ; Gao, Jing; Yu, Hao; Zhou, Liya; Ma, Li; Jiang, Yanjun;     Show all 6 Authors
Source:
Biochemical engineering journal 2017 v.117 pp. 92-101
ISSN:
1369-703X
Subject:
catalytic activity; crosslinking; glutaraldehyde; immobilized enzymes; nanoparticles; nicotinamide; pH; porous media; silica; storage quality; surface area; temperature
Abstract:
... Tannic-acid-templated magnetic mesoporous silica nanoparticles (TA-MMSNs) were synthesized for the first time. The TA-MMSNs were monodisperse spherical particles with a diameter of around 250nm and a magnetization saturation value of 35.26 emu/g. The specific surface area of TA-MMSNs was 423.4m²/g, and the diameter and cumulative volume of the pores were 9.349nm and 1.071cm³/g, respectively. The T ...
DOI:
10.1016/j.bej.2016.10.005

5. Immobilization of Glucose Oxidase on Polydopamine-Functionalized Graphene Oxide

Author:
He, Ying,   et al.   ; Zhou, Liya; Jiang, Yanjun; Ma, Li; Gao, Jing;     Show all 5 Authors
Source:
Applied biochemistry and biotechnology 2015 v.175 no.2 pp. 1007-1017
ISSN:
0273-2289
Subject:
biocomposites; biopolymers; biosensors; dopamine; glucose oxidase; graphene; immobilized enzymes; microbial fuel cells; pH; storage quality
Abstract:
... In this study, graphene oxide (GO) was modified with dopamine to create a matrix for enzyme immobilization. Dopamine can self-polymerize to get polydopamine (PDA) and coated on GO surface. At the same time, GO was reduced to get PDA/rGO biocomposite. The PDA/rGO may offer adherent surface for enzyme immobilization. Glucose oxidase (GOD), an oxidoreductase, was chosen as model enzyme and can be eas ...
DOI:
10.1007/s12010-014-1324-1

6. Protein-based inverse opals: A novel support for enzyme immobilization

Author:
He, Ying,   et al.   ; Jiang, Yanjun; Sun, Wenya; Wang, Yaping; Wang, Lihui; Zhou, Liya; Gao, Jing; Ma, Li; Zhang, Xu;     Show all 9 Authors
Source:
Enzyme and Microbial Technology 2017 v.96 pp. 42-46
ISSN:
0141-0229
Subject:
catalytic activity; crosslinking; hydrolysis; immobilized enzymes; pH; penicillin amidase
Abstract:
... In this study, protein-based inverse opals were prepared for the first time by using the colloidal crystal templating method. The preparation process involved three steps including filling the templates with protein molecules, crosslinking, and template removal. The obtained inverse opals were used to immobilize Penicillin G acylase (PGA) because of its intrinsic biocompatible property. The immobi ...
DOI:
10.1016/j.enzmictec.2016.08.021

7. Improved Performance of Lipase Immobilized on Tannic Acid-Templated Mesoporous Silica Nanoparticles

Author:
He, Ying,   et al.   ; Jiang, Yanjun; Sun, Wenya; Zhou, Liya; Ma, Li; Gao, Jing;     Show all 6 Authors
Source:
Applied biochemistry and biotechnology 2016 v.179 no.7 pp. 1155-1169
ISSN:
0273-2289
Subject:
Burkholderia cepacia; adsorption; biodiesel; carboxylic ester hydrolases; esterification; ethanol; fuel production; hexane; immobilized enzymes; iso-octanes; methanol; nanoparticles; porosity; porous media; recycling; scanning electron microscopy; silica; solvents; surface area; tannins; tetrahydrofuran; thermal stability; transesterification; transmission electron microscopy
Abstract:
... Mesoporous silica nanoparticles were synthesized by using tannic acid as a pore-forming agent, which is an environmentally friendly, cheap, and non-surfactant template. SEM and TEM images indicated that the tannic acid-templated mesoporous silica nanoparticles (TA-MSNs) are monodisperse spherical-like particles with an average diameter of 195 ± 16 nm. The Brunauer–Emmett–Teller (BET) results showe ...
DOI:
10.1007/s12010-016-2056-1

8. Co-aggregation of Laccase and Nature Egg White: a Simple Method to Prepare Stable and Recyclable Biocatalyst

Author:
He, Ying,   et al.   ; Jiang, Yanjun; Wang, Qi; Zhou, Liya; Gao, Jing;     Show all 5 Authors
Source:
Applied biochemistry and biotechnology 2014 v.172 no.5 pp. 2496-2506
ISSN:
0273-2289
Subject:
2,4-dichlorophenol; bovine serum albumin; crosslinking; egg albumen; glutaraldehyde; immobilized enzymes; laccase; temperature; thermal stability; trypsin; urea
Abstract:
... Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bovine serum albumin, etc.) in the preparation of laccase CLEAs (CLEAs-egg). The ef ...
DOI:
10.1007/s12010-013-0697-x
PubMed:
24398920

9. Immobilization of penicillin G acylase on macrocellular heterogeneous silica-based monoliths

Author:
He, Ying,   et al.   ; Wang, Hua; Jiang, Yanjun; Zhou, Liya; Gao, Jing;     Show all 5 Authors
Source:
Journal of Molecular Catalysis. B, Enzymatic 2013 v.96 pp. 1-5
ISSN:
1381-1177
Subject:
benzylpenicillin; catalytic activity; epoxides; immobilized enzymes; pH; silicon; storage quality; temperature
Abstract:
... A novel material labeled as Si(HIPEs) which possesses macroporosity and mesostructuration was synthesized and functionalized with amino or epoxy groups. The native Si(HIPEs) and functionalized Si(HIPEs) were employed as supports for penicillin G acylase (PGA) immobilization. The effect of pH and temperature on the activity of immobilized PGA was investigated. The reusability, operational stability ...
DOI:
10.1016/j.molcatb.2013.06.005

10. Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica particles: A robust biocatalyst system for biodiesel production

Author:
He, Ying,   et al.   ; Jiang, Yanjun; Liu, Xinlong; Chen, Yafei; Zhou, Liya; Ma, Li; Gao, Jing;     Show all 7 Authors
Source:
Bioresource technology 2014 v.153 pp. 278-283
ISSN:
0960-8524
Subject:
Jatropha curcas; biocatalysts; biodiesel; buffers; carboxylic ester hydrolases; emulsions; esterification; ethanol; fuel production; immobilized enzymes; industrial applications; oils; oleic acid; pH; phosphates; porous media; temperature
Abstract:
... A novel catalytic system of Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica was constructed (named LP@PE) and used as biocatalyst for biodiesel production. The reaction parameters were optimized and the optimum conditions were as follows: the water fraction 0.65%, molar ratio of ethanol to oleic acid 2:1, immobilized lipase particles 150mg, phosphate buffer pH 7 ...
DOI:
10.1016/j.biortech.2013.12.001

11. Reversible, selective immobilization of nuclease P1 from a crude enzyme solution on a weak base anion resin activated by polyethylenimine

Author:
He, Ying,   et al.   ; Li, Bingbing; Chen, Yong; Cao, Zhi; Niu, Huanqing; Liu, Dong; Chen, Xiaochun; Wu, Jinglan; Xie, Jingjing; Zhuang, Wei; Ying, Hanjie;     Show all 11 Authors
Source:
Journal of Molecular Catalysis. B, Enzymatic 2014 v.101 pp. 92-100
ISSN:
1381-1177
Subject:
activation energy; adsorption; catalytic activity; functional properties; immobilized enzymes; mass transfer; pH; temperature
Abstract:
... The reversible immobilization of crude nuclease P1 fermented by Penicllium citrinum and used without further purification was performed via adsorption on a weak base anion resin activated by polyethylenimine (PEI). The immobilization conditions, including PEI concentration, the amount of support, the immobilization time, and the reusability of the PEI-activated resin were investigated. The results ...
DOI:
10.1016/j.molcatb.2013.12.021