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1.  Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Author:
Y.X. Yang; J.Q. Wang; D.P. Bu; S.S. Li; T.J. Yuan; L.Y Zhou; J.H. Yang; P. Sun
Source:
Czech Journal of Animal Science v.57 no.10 pp. 481-489
ISSN:
1212-1819
Subject:
amyloid; animal science; apolipoprotein E; blood serum; haptoglobins; immune system; immunoglobulins; lactation; liquid chromatography; prealbumin; protein content; proteomics; tandem mass spectrometry; two-dimensional gel electrophoresis
Abstract:
... The transition period is the most critical time of the cow’s lactation cycle that is associated with the onset of mastitis. In this study, changes of plasma proteins in cows (n = 12) with or without subclinical mastitis after calving were determined by two-dimensional electrophoresis (2-DE), which detected 18 spots with variations in protein spots abundance. These spots were identified by li ...
DOI:
10.17221/6348-cjas

2. (+)-Dihydrorobinetin: a Marker of Vinegar Aging in Acacia (Robinia pseudoacacia) Wood

Author:
Cerezo, Ana B.; Espartero, Jose L.; Winterhalter, Peter; Garcia-Parrilla, M. Carmen; Troncoso, Ana M.
Source:
Journal of agricultural and food chemistry 2009 v.57 no.20 pp. 9551–9554
ISSN:
0021-8561
Subject:
vinegars; fermentation; Robinia pseudoacacia; wood products; biomarkers; food composition; chemical composition; liquid chromatography; nuclear magnetic resonance spectroscopy; chemical structure; optical properties; flavonols; temporal variation; antioxidant activity; product authenticity
Abstract:
... The use of acacia wood for the aging of vinegars is increasing because the efficient air transfer through the pores permits a good acetification rate. In this study, vinegars aged in acacia (Robinia pseudoacacia) wood barrels were analyzed and found to contain a characteristic compound, which increased during the aging process. This so far unknown compound was isolated by semipreparative LC and st ...
DOI:
10.1021/jf901794c
PubMed:
19785439

3. (-)-Calcaridine B, a new chiral aminoimidazole-containing alkaloid from the marine sponge Leucetta chagosensis

Author:
Wei-Zhuo Tang; Zhong-Zhen Yang; Fan Sun; Shu-Ping Wang; Fan Yang; Wei-Hua Jiao; Hou-Wen Lin
Source:
Journal of Asian natural products research 2019 v.21 no.11 pp. 1123-1128
ISSN:
1477-2213
Subject:
Porifera; alkaloids; cytotoxicity; human cell lines; imidazole; inhibitory concentration 50; liquid chromatography; neoplasm cells; nuclear magnetic resonance spectroscopy; spectral analysis
Abstract:
... LC-DAD/MS-based dereplication of organic extract of a calcareous sponge Leucetta chagosensis afforded one new chiral aminoimidazole-containing alkaloid, (-)-calcaridine B (1), along with one achiral imidazole analog leucettamine E (2) as well as one known imidazole derivative (2E, 9E)-pyronaamidine-9-(N-methylimine) (3). Their structures were elucidated on the basis of NMR spectroscopic analyses, ...
DOI:
10.1080/10286020.2018.1499729

4. (-)-Leucophyllone, a Tirucallane Triterpenoid from Cornus walteri, Enhances Insulin Secretion in INS-1 Cells

Author:
Dahae Lee; Ki Hyun Kim; Taesu Jang; Ki Sung Kang
Source:
Plants 2021 v.10 no.3 pp. -
ISSN:
2223-7747
Subject:
Cornus walteri; bark; cytotoxicity; hypoglycemic agents; insulin receptor substrate proteins; insulin secretion; liquid chromatography; mass spectrometry; mechanism of action; nuclear magnetic resonance spectroscopy; phosphatidylinositol 3-kinase; phytochemicals; rats; triterpenoids
Abstract:
... Phytochemical examination of the MeOH extract from the stems and stem bark of Cornus walteri (Cornaceae) led to the isolation and verification of a tirucallane triterpenoid, (-)-leucophyllone, as a major component. Its structure was elucidated using NMR spectroscopy and liquid chromatography–mass spectrometry. The effect of (-)-leucophyllone on insulin secretion in INS-1 cells was investigated. (- ...
DOI:
10.3390/plants10030431

5. (1 leads to 6)-beta-D-glucan as cell wall receptor for Pichia membranifaciens killer toxin

Author:
Santos, A.; Marquina, D.; Leal, J.A.; Peinado, J.M.
Source:
Applied and environmental microbiology 2000 v.66 no.5 pp. 1809-1813
ISSN:
0099-2240
Subject:
Candida boidinii; Penicillium; Pichia membranifaciens; adsorption; beta-glucans; cell wall components; cell walls; fermentation; gas chromatography; industrial applications; infrared spectroscopy; liquid chromatography; nuclear magnetic resonance spectroscopy; olives; oxidation; sodium chloride; yeasts
Abstract:
... The killer toxin from Pichia membranifaciens CYC 1106, a yeast isolated from fermenting olive brines, binds primarily to the (1 leads to 6)-beta-D-glucan of the cell wall of a sensitive yeast (Candida boidinii IGC 3430). The (1 leads to 6)-beta-D-glucan was purified from cell walls of C. boidinii by alkali and hot-acetic acid extraction, a procedure which solubilizes glucans. The major fraction of ...
DOI:
10.1128/AEM.66.5.1809-1813.2000
PubMed:
10788343
PubMed Central:
PMC101416

6. (3ξ,4ξ,5ξ,6ξ,7ξ,11ξ)-3,6-Dihydroxy-8-oxo-9-eremophilene-12-oic Acid, a New Phytotoxin of Alternaria alternata ssp. tenuissima Isolates Associated with Fruit Spots on Apple (Malus × domestica Borkh.)

Author:
Sabine Oettl; Mattia Bosello; Klaus Marschall; Andreas Gallmetzer; Yazmid Reyes-Domínguez; Christoph Kreutz; Martin Tollinger; Peter Robatscher; Michael Oberhuber
Source:
Journal of agricultural and food chemistry 2021 v.69 no.48 pp. 14445-14458
ISSN:
1520-5118
Subject:
Alternaria alternata; Malus domestica; apples; conidia; food chemistry; fractionation; fruit quality; fruits; fungi; host specificity; leaves; liquid chromatography; mass spectrometry; metabolites; nuclear magnetic resonance spectroscopy; pathogenicity; pathotypes; phytotoxins; Italy
Abstract:
... Alternaria sp. infections on apple (Malus × domestica Borkh.) lead to impaired fruit quality and yield losses by leaf blotches and fruit spots, caused by host-specific toxins (HSTs) of the Alternaria apple pathotype like AM-toxins. Fungal isolates were obtained during severe outbreaks on cv. Gala, Golden Delicious, and Cripps Pink⁽ᶜᵒᵛ⁾/Rosy Glow⁽ᶜᵒᵛ⁾ in South Tyrol and other regions in northern It ...
DOI:
10.1021/acs.jafc.1c02847

7. (Poly)phenolic fingerprint and chemometric analysis of white (Morus alba L.) and black (Morus nigra L.) mulberry leaves by using a non-targeted UHPLC–MS approach

Author:
Eva Mª. Sánchez-Salcedo; Michele Tassotti; Daniele Del Rio; Francisca Hernández; Juan José Martínez; Pedro Mena
Source:
Food chemistry 2016 v.212 pp. 250-255
ISSN:
0308-8146
Subject:
kaempferol; cluster analysis; chlorogenic acid; mass spectrometry; liquid chromatography; quality control; Morus nigra; Morus alba; clones; leaves; intraspecific variation; principal component analysis; chemometrics; quercetin; bioactive properties; glycosylation; mulberries; phenolic acids; germplasm screening; Spain
Abstract:
... This study reports the (poly)phenolic fingerprinting and chemometric discrimination of leaves of eight mulberry clones from Morus alba and Morus nigra cultivated in Spain. UHPLC–MSn (Ultra High Performance Liquid Chromatography–Mass Spectrometry) high-throughput analysis allowed the tentative identification of a total of 31 compounds. The phenolic profile of mulberry leaf was characterized by the ...
DOI:
10.1016/j.foodchem.2016.05.121

8. (S)-1-(4-Dimethylaminophenylcarbonyl)-3-aminopyrrolidine: A derivatization reagent for enantiomeric separation and sensitive detection of chiral carboxylic acids by LC/ESI-MS/MS

Author:
Shoujiro Ogawa; Hiroaki Tadokoro; Maho Sato; Takehisa Hanawa; Tatsuya Higashi
Source:
Journal of Chromatography B 2013 v.940 pp. 7-14
ISSN:
1570-0232
Subject:
ambient temperature; carboxylic acids; derivatization; detection limit; electrospray ionization mass spectrometry; humans; ibuprofen; liquid chromatography; monitoring; saliva; tandem mass spectrometry
Abstract:
... A novel derivatization reagent, (S)-1-(4-dimethylaminophenylcarbonyl)-3-aminopyrrolidine (1-DAPAP), was developed for increasing the detection sensitivity and enantiomeric separation of chiral carboxylic acids by liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI-MS/MS). 1-DAPAP reacted with carboxylic acids at room temperature within 5min in the presence of 4-(4,6-dime ...
DOI:
10.1016/j.jchromb.2013.09.025

9. (Un)targeted Scanning of Locks of Hair for Drugs of Abuse by Direct Analysis in Real Time–High-Resolution Mass Spectrometry

Author:
Duvivier Wilco F.; van Putten Marc R.; van Beek Teris A.; Nielen Michel W. F.
Source:
Analytical chemistry 2016 v.88 no.4 pp. 2489-2496
ISSN:
1520-6882
Subject:
cocaine; detection limit; forensic sciences; hairs; illicit drugs; image analysis; liquid chromatography; tandem mass spectrometry; thermal degradation
Abstract:
... Forensic hair evidence can be used to obtain retrospective timelines of drug use by analysis of hair segments. However, this is a laborious and time-consuming process, and mass spectrometric (MS) imaging techniques, which show great potential for single-hair targeted analysis, are less useful due to differences in hair growth rate between individual hairs. As an alternative, a fast untargeted anal ...
DOI:
10.1021/acs.analchem.5b04759

10. (Un)targeted hair metabolomics: first considerations and systematic evaluation on the impact of sample preparation

Author:
Lisa Eisenbeiss; Andrea E. Steuer; Tina M. Binz; Markus R. Baumgartner; Thomas Kraemer
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.17 pp. 3963-3977
ISSN:
1618-2642
Subject:
acetone; acetonitrile; buffer index; buffers; cutting; decontamination; gas chromatography; grinding; homogenization; liquid chromatography; mass spectrometry; metabolites; metabolomics; methanol; methylene chloride; monitoring; pH; prediction; solvents; urine; washing
Abstract:
... The interest in metabolomic studies has rapidly increased over the past few years. Changes of endogenous compounds are typically detected in plasma or urine. However, the use of hair allows for long-term monitoring of metabolomic changes and has recently started being applied to metabolomic studies. Within the proposed study, we aimed for a systematical investigation of different pre-analytical pa ...
DOI:
10.1007/s00216-019-01873-4

11. 1 mm ID poly(styrene-co-divinylbenzene) monolithic columns for high-peak capacity one- and two-dimensional liquid chromatographic separations of intact proteins

Author:
Eeltink, Sebastiaan; Dolman, Sebastiaan; Detobel, Frederik; Desmet, Gert; Swart, Remco; Ursem, Mario
Source:
Journal of separation science 2009 v.32 no.15-16 pp. 2504-2509
ISSN:
1615-9306
Subject:
Escherichia coli; liquid chromatography; proteins
Abstract:
... The LC performance of a 1x50 mm polymer monolithic column format was demonstrated with high-peak capacity one- (1D) and offline two dimensional (2D) LC separations of intact proteins. After optimizing the RP 1D-LC conditions, including column temperature, flow rate and gradient time, a peak capacity of 475 was achieved within a 2-h analysis. The suitability of the monolithic column was also demons ...
DOI:
10.1002/jssc.200900068
PubMed:
19557812

12. 1,4-D-Glucan block copolymers: synthesis and comprehensive structural characterization

Author:
Payam Hashemi; Petra Mischnick
Source:
Analytical and bioanalytical chemistry 2020 v.412 no.7 pp. 1597-1610
ISSN:
1618-2642
Subject:
Fourier transform infrared spectroscopy; composite polymers; dissociation; electrospray ionization mass spectrometry; glucans; glycosylation; liquid chromatography; methylation; models; nuclear magnetic resonance spectroscopy; tandem mass spectrometry
Abstract:
... Multi-block glucans comprising permethylated and partially methylated blocks are compounds of interest. In order to monitor their formation by transglycosylation of corresponding starting glucans, a method has been developed and applied to model compounds. This method allows determining the average length of the blocks and the progress of incorporation of methyl blocks in partially methylated sequ ...
DOI:
10.1007/s00216-020-02388-z
PubMed:
31960080
PubMed Central:
PMC7026270

13. 1,5-Anhydro-d-glucitol in vitreous humor and cerebrospinal fluid — A helpful tool for identification of diabetes and diabetic coma post mortem

Author:
Konrad Sydow; Theresa Kueting; Frank Musshoff; Burkhard Madea; Cornelius Hess
Source:
Forensic science international 2018 v.289 pp. 397-407
ISSN:
0379-0738
Subject:
biomarkers; blood serum; cerebrospinal fluid; coma; death; diabetes mellitus; glucose; guidelines; hyperglycemia; liquid chromatography; mass spectrometry; patients; regression analysis; renal reabsorption
Abstract:
... Since there are no characteristic morphological findings post mortem diagnosis of diabetes mellitus and identification of diabetic coma need to be confirmed by suitable biomarkers. The postmortem identification of preexisting hyperglycemia or diabetic coma can be difficult if the matrices for the determination of the established biomarkers are not available or the obtained results are close to the ...
DOI:
10.1016/j.forsciint.2018.06.021

14. 1-Methylcyclopropene Preserves the Quality of Chive (Allium schoenoprasum L.) by Enhancing Its Antioxidant Capacities and Organosulfur Profile during Storage

Author:
Xiaomei Dai; Yaping Lu; Yuan Yang; Zhifang Yu
Source:
Foods 2021 v.10 no.8 pp. -
ISSN:
2304-8158
Subject:
1-methylcyclopropene; Allium schoenoprasum; ascorbic acid; cell membranes; chives; chlorophyll; free amino acids; fumigation; glutathione; liquid chromatography; peroxidase; storage time; sulfoxides; superoxide dismutase
Abstract:
... The quality, antioxidant capacities, and organosulfur profile of chives (Allium schoenoprasum L.) treated with 1-methylcyclopropene (1-MCP) during storage were investigated in this study. The 1-MCP treatment (100 μL/L, fumigation 12 h at 20 °C) effectively inhibited tissue respiration and H₂O₂ production, enhanced the ascorbic acid (ASA) and glutathione (GSH) content, and promoted the activity of ...
DOI:
10.3390/foods10081792

15. 1-Methylcyclopropene Treatment on Phenolics and the Antioxidant System in Postharvest Peach Combined with the Liquid Chromatography/Mass Spectrometry Technique

Author:
Xiaoqin Wu; Xiujuan An; Mingliang Yu; Ruijuan Ma; Zhifang Yu
Source:
Journal of agricultural and food chemistry 2018 v.66 no.25 pp. 6364-6372
ISSN:
1520-5118
Subject:
1-methylcyclopropene; 2,2-diphenyl-1-picrylhydrazyl; anthocyanins; antioxidant activity; biochemical pathways; biosynthesis; carbon dioxide production; color; enzymes; ethylene; hydroxyl radicals; kaempferol; liquid chromatography; mass spectrometry; peaches; reverse transcriptase polymerase chain reaction; storage time; superoxide anion; transcription (genetics)
Abstract:
... In the present study, the potential effect of 1-methylcyclopropene (1-MCP) treatment on phenolics and antioxidant capacity in postharvest peach was assessed. Peach fruit (cv. Xiahui-8) treated with 1-MCP or without treatment was stored in 25 °C for 2, 4, 6, and 8 days. The phenolic composition and change trend were evaluated by liquid chromatography/mass spectrometry. The reactive oxygen species p ...
DOI:
10.1021/acs.jafc.8b01757

16. 1.1μm Superficially porous particles for liquid chromatography

Author:
Laura E. Blue; James W. Jorgenson
Source:
Journal of chromatography 2015 v.1380 pp. 71-80
ISSN:
0021-9673
Subject:
acetone; electrochemistry; light microscopy; liquid chromatography; mass transfer; methanol; particle size; slurries; solvents
Abstract:
... The predicted advantages of superficially porous particles over totally porous particles are decreased eddy dispersion, longitudinal diffusion, and resistance to mass transfer contributions to the theoretical plate height. While sub-2 micron superficially porous particles are commercially available, further improvements in performance are predicted by decreasing the particle diameter and decreasin ...
DOI:
10.1016/j.chroma.2014.12.055

17. 1.1μm superficially porous particles for liquid chromatography. Part I: Synthesis and particle structure characterization

Author:
Laura E. Blue; James W. Jorgenson
Source:
Journal of chromatography 2011 v.1218 no.44 pp. 7989-7995
ISSN:
0021-9673
Subject:
coatings; drying; liquid chromatography; mass transfer; packaging materials; particle size distribution; silica; surface area; temperature
Abstract:
... Superficially porous particles are characterized by a non-porous particle core surrounded by a thin porous layer. Superficially porous particles have been shown to have chromatographic advantages over traditional totally porous particles by reducing the resistance to mass transfer and the eddy diffusion contributions to the theoretical plate height, particularly for biomolecule separations. Curren ...
DOI:
10.1016/j.chroma.2011.09.004

18. 10years of MS instrumental developments – Impact on LC–MS/MS in clinical chemistry

Author:
Markus Himmelsbach
Source:
Journal of chromatography 2012 v.883-884 no. pp. 3-17
ISSN:
1570-0232
Subject:
atmospheric pressure; biochemistry; chemistry; liquid chromatography; medicine; spectrometers; tandem mass spectrometry
Abstract:
... The combination of liquid chromatography and mass spectrometry (LC–MS) is a powerful and indispensable analytical tool that is widely applied in many areas of chemistry, medicine, pharmaceutics and biochemistry. In this review recent MS instrumental developments are presented as part of a special issue covering various aspects of liquid chromatography tandem mass spectrometry (LC–MS/MS) in clinica ...
DOI:
10.1016/j.jchromb.2011.11.038

19. 11-Nor-9-carboxy-∆9-tetrahydrocannabinol quantification in human oral fluid by liquid chromatography–tandem mass spectrometry

Author:
Karl B. Scheidweiler; Sarah K. Himes; Xiaohong Chen; Hua-Fen Liu; Marilyn A. Huestis
Source:
Analytical and bioanalytical chemistry 2013 v.405 no.18 pp. 6019-6027
ISSN:
1618-2642
Subject:
Cannabis; acetic acid; cannabinoids; derivatization; drug therapy; drugs; humans; ionization; liquid chromatography; methanol; monitoring; pain; quality control; solid phase extraction; tandem mass spectrometry; working conditions
Abstract:
... Currently, ∆9-tetrahydrocannabinol (THC) is the analyte quantified for oral fluid cannabinoid monitoring. The potential for false-positive oral fluid cannabinoid results from passive exposure to THC-laden cannabis smoke raises concerns for this promising new monitoring technology. Oral fluid 11-nor-9-carboxy-∆9-tetrahydrocannabinol (THCCOOH) is proposed as a marker of cannabis intake since it is n ...
DOI:
10.1007/s00216-013-7027-6
PubMed:
23681203
PubMed Central:
PMC3773502

20. 122 Proteome of extracellular vesicles from follicular fluid of bovine 3- to 6-mm follicles: Similarity and specificity compared with granulosa cells

Author:
S. Uzbekova; C. Alminana-Brines; V. Labas; L. Combes-Soia; I. I. Kireev; A.-P. Teixeira-Gomes; R. E. Uzbekov; G. N. Singina
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 188
ISSN:
1031-3613
Subject:
NAD (coenzyme); RNA-binding proteins; antioxidant activity; carbon; cattle; cell adhesion; cell communication; cell cycle; cell proliferation; cholesterol; computer software; embryo (animal); endocytosis; endoplasmic reticulum; exosomes; fatty acids; follicular fluid; gene ontology; granulosa cells; liquid chromatography; messenger RNA; metabolism; mitochondria; non-coding RNA; oocytes; peptides; polyacrylamide gel electrophoresis; protein content; protein folding; protein isoforms; proteolysis; proteome; ribosomal RNA; ribosomal proteins; sodium dodecyl sulfate; tandem mass spectrometry; translation (genetics); transmission electron microscopy; trypsin
Abstract:
... Extracellular vesicles (EVs) are released from cells and are present in most bodily fluids. Small EVs, 30-150nm in diameter, known as exosomes, transport regulatory noncoding RNA, lipids, and proteins, and thus participate in cell-to-cell communications and signalling. Exosomes from bovine ovarian follicular fluid (FF) can stimulate proliferation of granulosa cells (GCs), cellular uptake, and cumu ...
DOI:
10.1071/RDv32n2Ab122

21. 13-Desmethyl spirolide-c and 13,19-didesmethyl spirolide-c trans-epithelial permeabilities: Human intestinal permeability modelling

Author:
Begoña Espiña; Paz Otero; M. Carmen Louzao; Amparo Alfonso; Luis M. Botana
Source:
Toxicology 2011 v.287 no.1-3 pp. 69-75
ISSN:
0300-483X
Subject:
Alexandrium; circulatory system; drugs; electrical resistance; humans; intestinal absorption; intestinal mucosa; liquid chromatography; mice; models; molluscs; oral administration; permeability; pharmacokinetics; prediction; tandem mass spectrometry; toxicity; toxicology
Abstract:
... Human intestinal permeability prediction is an increasingly important field that helps to explain how efficient the absorption of drugs is. Spirolides, cyclic imines produced by dinoflagellates from the genera Alexandrium, can be accumulated in mollusks usually consumed by humans. These compounds exert neurological symptoms when injected intra-peritoneally in mice, although they seem to be less to ...
DOI:
10.1016/j.tox.2011.06.003

22. 13C-Enrichment of Urinary Uric Acid after l-[Ring-2-13C]Histidine Dose in Adult Humans

Author:
Tsunenobu Tamura; Joseph E. Baggott
Source:
Nutrients 2015 v.7 no.1 pp. 697-705
ISSN:
2072-6643
Subject:
adults; autoimmune diseases; biosynthesis; carbon; circadian rhythm; drugs; histidine; humans; liquid chromatography; neoplasms; normal values; spectroscopy; uric acid; urine
Abstract:
... We determined whether ring-2 carbon of histidine is folate-dependently transferred to carbons 8 (C₈) and/or 2 (C₂) in urinary uric acid in humans. Two adults collected each urine void for four days. Aliquots of urine for the first day were used for baseline values; then the subjects ingested 0.7 g (3.3 mmol) of l-[ring-2-13C]histidine and collected urine for three experimental days. Aliquots were ...
DOI:
10.3390/nu7010697
PubMed:
25608940
PubMed Central:
PMC4303862

23. 14 Evaluation of the absorption of various nutraceuticals in horses at different fed rates

Author:
R.D. Jacobs; M.E. Gordon; M.L. Jerina
Source:
Journal of equine veterinary science 2021 v.100 pp. 103477
ISSN:
0737-0806
Subject:
absorption; benzoic acid; biomarkers; blood serum; collagen; cranberries; dietary supplements; epigallocatechin gallate; green tea; hay; horses; hydrolysis; liquid chromatography; mass spectrometry; metabolites; turmeric; veterinary medicine
Abstract:
... Nutraceuticals are non-nutritive additives aimed at providing health benefits to the horse. Determining the efficacy of these nutraceuticals should start with an understanding of their absorption when administered through feeding. To determine the in vivo absorption of a variety of nutraceutical compounds, a trial was conducted in which various products were fed at increasing concentrations and bl ...
DOI:
10.1016/j.jevs.2021.103477

24. 14-3-3 is a VP3-binding protein involved in Macrobrachium rosenbergii Taihu virus infection in shrimp

Author:
Xiaoyi Pan; Qiang Gao; Jinyu Shen; Ting Xu
Source:
Developmental and comparative immunology 2021 v.122 pp. 104139
ISSN:
0145-305X
Subject:
Macrobrachium rosenbergii; capsid; liquid chromatography; mortality; pathogenesis; pathogens; precipitin tests; shrimp; tandem mass spectrometry; viruses
Abstract:
... Macrobrachium rosenbergii Taihu virus (MrTV) is a fierce pathogen that causes high mortality in M. rosenbergii larvae. Little is known about the pathogenesis of MrTV and host-virus interactions. In this study, a virus overlay protein binding assay (VOPBA), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, was carried out to search for novel host molecules that bind wi ...
DOI:
10.1016/j.dci.2021.104139

25. 14-3-3β/α-A interacts with glycoprotein of spring viremia of carp virus and positively affects viral entry

Author:
Buxin Chen; Chen Li; Yeda Wang; Yuanan Lu; Fang Wang; Xueqin Liu
Source:
Fish & shellfish immunology 2018 v.81 pp. 438-444
ISSN:
1050-4648
Subject:
messenger RNA; virion; Cyprinus carpio; shellfish; endocytosis; gene expression; tandem mass spectrometry; immunology; mortality; fish; Carp sprivivirus; gene expression regulation; liquid chromatography; glycoproteins; pathogens
Abstract:
... Spring viremia of carp virus (SVCV) is a fierce pathogen causing high mortality in the common carp. The glycoprotein (G protein) of SVCV is a pivotal component of the viral structure, located in the surface of the virion, and plays a key role in viral endocytosis. In this study, tandem affinity purification (TAP) followed by mass spectrometry analysis (LC-MS/MS) was carried out to search for novel ...
DOI:
10.1016/j.fsi.2018.04.031

26. 141 Diet supplementation alters oocyte lipid content and developmental competence in mares

Author:
G. Catandi; J. Stokes; L. Maclellan; C. Broeckling; E. Carnevale
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 178
ISSN:
1031-3613
Subject:
alfalfa hay; blastocyst; corn oil; dietary supplements; embryogenesis; energy; estrus; grasses; ideal body weight; lipid content; liquid chromatography; mass spectrometry; oocytes; platinum; spermatozoa; stallions; t-test; triacylglycerols
Abstract:
... The equine oocyte is dense in lipids, which may serve as an energy source for oocyte maturation and later embryonic development. However, the association between lipid content and fertility remains to be determined, as does the extent that diet can modify oocyte lipids. We hypothesised that diet supplementation can alter the oocyte lipid profile and subsequent developmental potential. In Study 1, ...
DOI:
10.1071/RDv33n2Ab141

27. 142 Metabolomic analysis of fresh and frozen bovine seminal plasma: A preliminary study

Author:
M. A. Kosior; N. Pagano; A. Staropoli; C. De Canditiis; V. Longobardi; G. Zullo; F. Vinale; B. Gasparrini
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 197-198
ISSN:
1031-3613
Subject:
Holstein; artificial vagina; assisted reproductive technologies; biochemical pathways; bioinformatics; biomarkers; bulls; centrifugation; ceramides; cryopreservation; freeze-thaw cycles; freezing; frozen storage; liquid chromatography; male fertility; mass spectrometry; metabolites; metabolomics; methanol; oxidative stress; screening; seminal plasma; sperm motility; spermatozoa; storage temperature; t-test; Italy
Abstract:
... Sperm cryopreservation is essential for assisted reproduction; however, freezing induces biochemical and physical damage to the sperm membrane structures, negatively affecting sperm fertilising ability (Castro et al. 2006 J. Anim. Sci. Biotechnol. 5, 1-9). Metabolomics is the study of small molecules, commonly known as metabolites, which play essential roles in biological systems. Emerging researc ...
DOI:
10.1071/RDv32n2Ab142

28. A 15-min non-competitive homogeneous assay for microcystin and nodularin based on time-resolved Förster resonance energy transfer (TR-FRET)

Author:
Sultana Akter; Urpo Lamminmäki
Source:
Analytical and bioanalytical chemistry 2021 v.413 no.24 pp. 6159-6170
ISSN:
1618-2642
Subject:
World Health Organization; analytical chemistry; detection limit; energy transfer; europium; fluorescent dyes; guidelines; hepatotoxins; liquid chromatography; mass spectrometry; microcystin-LR; monoclonal antibodies; nodularin; water analysis
Abstract:
... Simple and rapid methods are required for screening and analysis of water samples to detect cyanobacterial cyclic peptide hepatotoxins: microcystin/nodularin. Previously, we reported a highly sensitive non-competitive heterogeneous assay for microcystin/nodularin utilizing a generic anti-immunocomplex (anti-IC) single-chain fragment of antibody variable domains (scFv) isolated from a synthetic ant ...
DOI:
10.1007/s00216-021-03375-8

29. 15‐F2t‐Isoprostane Concentrations and Oxidant Status in Lactating Dairy Cattle with Acute Coliform Mastitis

Author:
V. Mavangira; M.J. Mangual; J.C. Gandy; L.M. Sordillo
Source:
Journal of veterinary internal medicine 2016 v.30 no.1 pp. 339-347
ISSN:
0891-6640
Subject:
Holstein; animal tissues; biomarkers; case-control studies; coliform bacteria; correlation; dairy cows; enzyme-linked immunosorbent assay; humans; inflammation; lactation; liquid chromatography; mammary glands; mastitis; milk; oxidants; oxidative stress; tandem mass spectrometry; urine
Abstract:
... BACKGROUND: Severe mammary tissue damage during acute coliform mastitis in cattle is partially caused by oxidative stress. Although considered a gold standard biomarker in some human conditions, the utility of 15‐F₂ₜ‐Isoprostanes (15‐F₂ₜ‐Isop) in detecting oxidative stress in dairy cattle has not been validated. HYPOTHESIS: Concentrations of 15‐F₂ₜ‐Isop in plasma, urine, and milk correlate with ch ...
DOI:
10.1111/jvim.13793
PubMed:
26566597
PubMed Central:
PMC4913657
CHORUS:
10.1111/jvim.13793
Chorus Open Access:
10.1111/jvim.13793

30. 16-O-methylcafestol is present in ground roast Arabica coffees: Implications for authenticity testing

Author:
Yvonne Gunning; Marianne Defernez; Andrew D. Watson; Niles Beadman; Ian J. Colquhoun; Gwénaëlle Le Gall; Mark Philo; Hollie Garwood; David Williamson; Aaron P. Davis; E. Kate Kemsley
Source:
Food chemistry 2018 v.248 pp. 52-60
ISSN:
0308-8146
Subject:
adulterated products; lipophilicity; liquid chromatography; mass spectrometry; monitoring; nuclear magnetic resonance spectroscopy
Abstract:
... High-field and low-field proton NMR spectroscopy were used to analyse lipophilic extracts from ground roast coffees. Using a sample preparation method that produced concentrated extracts, a small marker peak at 3.16 ppm was observed in 30 Arabica coffees of assured origin. This signal has previously been believed absent from Arabicas, and has been used as a marker for detecting adulteration with r ...
DOI:
10.1016/j.foodchem.2017.12.034
PubMed:
29329870
PubMed Central:
PMC5774150

31. 17,17-Dialkyltetrabenzo[a,c,g,i]fluorenes with extremely high solid-state fluorescent quantum yields: relationship between crystal structure and fluorescent properties

Author:
Chitoshi Kitamura; Yusuke Tanigawa; Takashi Kobayashi; Hiroyoshi Naito; Hiroyuki Kurata; Takeshi Kawase
Source:
Tetrahedron 2012 v.68 no.6 pp. 1688-1694
ISSN:
0040-4020
Subject:
chemical reactions; crystal structure; crystals; fluorescence; halides; liquid chromatography; optical properties; organic compounds
Abstract:
... Six 17,17-dialkyltetrabenzo[a,c,g,i]fluorenes (dialkylTBFs) 2–7 (methyl to n-hexyl) were prepared by treatment of 17H-tetrabenzo[a,c,g,i]fluorene (TBF) 1 with n-alkyl halides under basic conditions in moderate yields upon column chromatography and recrystallization. All the compounds show intense blue fluorescence in solutions. On the other hand, in the solid state, the fluorescent spectra and the ...
DOI:
10.1016/j.tet.2011.12.069

32. IL- 17A-stimulated endothelial fatty acid β-oxidation promotes tumor angiogenesis

Author:
Ruirui Wang; Xiaohan Lou; Guang Feng; Jinfeng Chen; Linyu Zhu; Xiaomeng Liu; Xiaohan Yao; Pan Li; Jiajia Wan; Yi Zhang; Chen Ni; Zhihai Qin
Source:
Life sciences 2019 v.229 pp. 46-56
ISSN:
0024-3205
Subject:
AMP-activated protein kinase; Western blotting; angiogenesis; beta oxidation; fatty acids; human cell lines; human umbilical vein endothelial cells; humans; interleukin-17; liquid chromatography; lung neoplasms; mass spectrometry; mitochondria; models; neoplasm cells; signal transduction; therapeutics
Abstract:
... Tumor growth is an angiogenesis-dependent process that requires sustained new vessel growth. Interleukin-17 (IL-17A) is a key cytokine that modulates tumor progression. However, whether IL-17A affects the metabolism of endothelial cells is unknown.A xenograft model was established by implanting H460 (human lung cancer cell line) cells transfected with IL-17A-expressing or control vector. The effec ...
DOI:
10.1016/j.lfs.2019.05.030

33. 193 nm Ultraviolet Photodissociation Mass Spectrometry for Phosphopeptide Characterization in the Positive and Negative Ion Modes

Author:
Robinson Michelle R.; Taliaferro Juliana M.; Dalby Kevin N.; Brodbelt Jennifer S.
Source:
Journal of Proteome Research 2016 v.15 no.8 pp. 2739-2748
ISSN:
1535-3907
Subject:
casein; data collection; dissociation; energy; ionization; ions; liquid chromatography; phosphates; phosphopeptides; photolysis; proteome; proteomics; tandem mass spectrometry
Abstract:
... Advances in liquid chromatography tandem mass spectrometry (LC–MS/MS) have permitted phosphoproteomic analysis on a grand scale, but ongoing challenges specifically associated with confident phosphate localization continue to motivate the development of new fragmentation techniques. In the present study, ultraviolet photodissociation (UVPD) at 193 nm is evaluated for the characterization of phosph ...
DOI:
10.1021/acs.jproteome.6b00289
PubMed:
27425180
PubMed Central:
PMC5481846

34. 193 nm Ultraviolet Photodissociation Mass Spectrometry for the Structural Elucidation of Lipid A Compounds in Complex Mixtures

Author:
O’Brien John P.; Needham Brittany D.; Henderson Jeremy C.; Nowicki Emily M.; Trent M. Stephen; Brodbelt Jennifer S.
Source:
Analytical chemistry 2014 v.86 no.4 pp. 2138-2145
ISSN:
1520-6882
Subject:
Escherichia coli; Pseudomonas aeruginosa; Vibrio cholerae; chemical bonding; dissociation; energy; ions; lipid A; liquid chromatography; photolysis; spectrometers; tandem mass spectrometry; vaccine adjuvants; vaccine development
Abstract:
... Here we implement ultraviolet photodissociation (UVPD) in an online liquid chromatographic tandem mass spectrometry (MS/MS) strategy to support analysis of complex mixtures of lipid A combinatorially modified during development of vaccine adjuvants. UVPD mass spectrometry at 193 nm was utilized to characterize the structures and fragment ion types of lipid A from Escherichia coli, Vibrio cholerae, ...
DOI:
10.1021/ac403796n
PubMed:
24446701
PubMed Central:
PMC3958132

35. 1H NMR and LC-MS-based metabolomic approach for evaluation of the seasonality and viticultural practices in wines from São Francisco River Valley, a Brazilian semi-arid region

Author:
Elenilson G. Alves Filho; Lorena Mara A. Silva; Paulo R.V. Ribeiro; Edy S. de Brito; Guilherme J. Zocolo; Patrícia C. Souza-Leão; Aline T.B. Marques; Amanda L. Quintela; Flemming H. Larsen; Kirley M. Canuto
Source:
Food chemistry 2019 v.289 pp. 558-567
ISSN:
0308-8146
Subject:
caffeic acid; chemometrics; glycerol; harvest date; lactic acid; liquid chromatography; metabolomics; nuclear magnetic resonance spectroscopy; proline; river valleys; rootstocks; secondary metabolites; semiarid zones; succinic acid; tartaric acid; vines; viticulture; wine quality; wines; Brazil
Abstract:
... São Francisco River Valley (SFRV) is a wine-producing semi-arid region in Brazil. Therefore, we used a 1H NMR and UPLC-MS-based metabolomic approach coupled to chemometrics to evaluate the variability in Chenin Blanc and Syrah wines for two harvest seasons, two vine training system and six rootstocks. Overall, the secondary metabolites were influenced by the three factors studied, whereas the prim ...
DOI:
10.1016/j.foodchem.2019.03.103

36. 1H NMR and multi-technique data fusion as metabolomic tool for the classification of golden rums by multivariate statistical analysis

Author:
José Raúl Belmonte-Sánchez; Roberto Romero-González; José Luis Martínez Vidal; Francisco Javier Arrebola; Antonia Garrido Frenich
Source:
Food chemistry 2020 v.317 pp. 126363
ISSN:
0308-8146
Subject:
chemometrics; discriminant analysis; distillation; fermentation; least squares; liquid chromatography; mass spectrometry; metabolomics; nuclear magnetic resonance spectroscopy; prediction; principal component analysis; raw materials; rum
Abstract:
... ¹H NMR spectroscopy combined with chemometrics was applied for the first time for golden rum classification based on several factors as fermentation barrel, raw material, distillation method and aging. Principal component analysis (PCA) was used to assess the overall structure, and partial least square discriminant analysis (PLS-DA) was carried out for the analytical discrimination of rums. Additi ...
DOI:
10.1016/j.foodchem.2020.126363

37. 1H NMR based pharmacometabolomics analysis of metabolic phenotype on predicting metabolism characteristics of losartan in healthy volunteers

Author:
Chenjie He; Yongbin Liu; Yicheng Wang; Jie Tang; Zhirong Tan; Xi Li; Yao Chen; Yuanfei Huang; Xiaoping Chen; Dongsheng Ouyang; Honghao Zhou; Jingbo Peng
Source:
Journal of chromatography 2018 v.1095 pp. 15-23
ISSN:
1570-0232
Subject:
biomarkers; blood; citrates; clinical trials; creatine; creatinine; drugs; gluconeogenesis; glycolysis; lactic acid; least squares; lipid metabolism; lipids; liquid chromatography; males; mass spectrometry; metabolites; models; nuclear magnetic resonance spectroscopy; oral administration; pharmacokinetics; phenotype; phosphorylcholine; prediction; serine; threonine
Abstract:
... Inter-individual variability in drug metabolism and disposition is common in both preclinical and clinical researches. Losartan and its active metabolite EXP3174 present a high degree of inter-individual differences in blood concentrations that affect drug efficacy and side effect. Pharmacometabolomics has been increasingly applied on predicting the drug responses by analyzing the differences in m ...
DOI:
10.1016/j.jchromb.2018.07.016

38. 1H-NMR metabolomics-based classification of Japanese sake and comparative metabolome analysis by gas chromatography–mass spectrometry

Author:
Yuji Hayashi; Takanori Komatsu; Kazuhiro Iwashita; Eiichiro Fukusaki
Source:
Journal of bioscience and bioengineering 2021 v.131 no.5 pp. 557-564
ISSN:
1389-1723
Subject:
Japan; ethanol; gas chromatography-mass spectrometry; liquid chromatography; metabolome; metabolomics; models; nuclear magnetic resonance spectroscopy; principal component analysis; rice; sake
Abstract:
... Six categories of Japanese sake have been established by the National Tax Agency of Japan. In this system, the rice polishing ratio and the addition of alcohol are the main criteria for classification. The most common nuclear magnetic resonance (NMR) spectrometry method is ¹H-NMR, and has higher throughput than gas chromatography–mass spectrometry (GC–MS) or liquid chromatography–mass spectrometry ...
DOI:
10.1016/j.jbiosc.2020.12.008

39. 1H‐NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm. f) Lindau water extract against nitric oxide production in LPS‐IFN‐γ activated RAW 264.7 macrophages

Author:
Leng Wei Khoo; Audrey Siew Foong Kow; Maulidiani Maulidiani; May Yen Ang; Won Yin Chew; Ming Tatt Lee; Chin Ping Tan; Khozirah Shaari; Chau Ling Tham; Faridah Abas
Source:
Phytochemical analysis 2019 v.30 no.1 pp. 46-61
ISSN:
0958-0344
Subject:
Clinacanthus nutans; air drying; amino acids; anti-inflammatory activity; biosynthesis; chemical composition; flavones; glucosides; herbal medicines; inhibitory concentration 50; least squares; leaves; liquid chromatography; macrophages; metabolites; metabolomics; nitric oxide; nuclear magnetic resonance spectroscopy; phytosterols; protective effect; shrubs; solvents; sonication; sulfur; tandem mass spectrometry; triterpenoids
Abstract:
... INTRODUCTION: Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source. Its anti‐inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post‐harvesting stage. OBJECTIVE: Evaluate the relationshi ...
DOI:
10.1002/pca.2789

40. 2,5-Diketopiperazines Produced by Bacillus pumilus During Bacteriolysis of Arthrobacter citreus

Author:
Christiane Brack; Annett Mikolasch; Frieder Schauer
Source:
Marine biotechnology 2014 v.16 no.4 pp. 385-395
ISSN:
1436-2228
Subject:
Arthrobacter citreus; Bacillus pumilus; antibiotics; bacteria; bioactive properties; gas chromatography; liquid chromatography; mass spectrometry; pasteurization
Abstract:
... We report the detection by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry analyses of the secreted 2,5-diketopiperazines (DKPs) cyclo(-Ala-Pro), cyclo(-Gly-Pro), cyclo(-Val-Pro), cyclo(-Ile-Pro), cyclo(-Leu-Pro), cyclo(-Pro-Pro), cyclo(-HyP-Pro), cyclo(-Met-Pro), and cyclo(-Phe-Pro) produced by Bacillus pumilus. The study focuses on a marine isolate and a laborato ...
DOI:
10.1007/s10126-014-9559-y
PubMed:
24449388

41. 2-Amino-3-(Oxirane-2,3-Dicarboxamido)-Propanoyl-Valine, an Effective Peptide Antibiotic from the Epiphyte Pantoea agglomerans 48b/90

Author:
Sammer, Ulrike F.; Völksch, Beate; Möllmann, Ute; Schmidtke, Michaela; Spiteller, Peter; Spiteller, Michael; Spiteller, Dieter
Source:
Applied and environmental microbiology 2009 v.75 no.24 pp. 7710-7717
ISSN:
0099-2240
Subject:
Candida albicans; Erwinia amylovora; Pantoea agglomerans; Pseudomonas syringae; Serratia plymuthica; anion exchange; apples; biological control; biological control agents; epiphytes; fractionation; human growth; hydrophobicity; ionization; leaves; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; peptide antibiotics; plant pathogenic bacteria; soybeans
Abstract:
... The epiphyte Pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the Enterobacteriaceae, as does the plant pathogen Erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. Since P. agglomerans efficiently antagonizes phytopathogenic bacteria, the P. agglomerans strain C9-1 is used as a biocontrol agent (Bli ...
DOI:
10.1128/AEM.01244-09
PubMed:
19820144
PubMed Central:
PMC2794118

42. 2-Arachidonoylglycerol is a substrate for butyrylcholinesterase: A potential mechanism for extracellular endocannabinoid regulation

Author:
Jason Barricklow; Matthew Blatnik
Source:
Archives of biochemistry and biophysics 2013 v.536 no.1 pp. 1-5
ISSN:
0003-9861
Subject:
acylglycerol lipase; arachidonic acid; horses; humans; hydrolysis; ionization; liquid chromatography; monitoring; pH; serine; tandem mass spectrometry
Abstract:
... 2-Arachidonoylglycerol (2-AG) is a component of the endocannabinoid receptor pathway and is primarily hydrolyzed by monoacylglycerol lipase (MAGL) in vivo. We found that the non-specific serine esterase, butyrylcholinesterase (BChE), can hydrolyze 2-AG with reasonable affinity and may present a new compensatory mechanism for endocannabinoid regulation. In vitro hydrolysis reactions of 2-AG with eq ...
DOI:
10.1016/j.abb.2013.05.003

43. 2-D hydrophilic interaction liquid chromatography-RP separation in urinary proteomics - Minimizing variability through improved downstream workflow compatibility

Author:
Loftheim, Håvard; Nguyen, Thien Duc; Malerød, Helle; Lundanes, Elsa; Åsberg, Anders; Reubsaet, Léon
Source:
Journal of separation science 2010 v.33 no.6-7 pp. 864-872
ISSN:
1615-9306
Subject:
equipment; filtration; hydrophilic interactions; liquid chromatography; patients; peptides; proteins; proteomics; trypsin; urea; urine
Abstract:
... Optimization of every step in a bottom-up urinary proteomics approach was studied with respect to maximize the protein recovery and making the downstream steps in the workflow fully compatible without compromising on the amount of information obtained. Sample enrichment and desalting using centrifugal filtration (5 kDa cut-off) yielded protein recoveries up to 97% when 8 M urea was used. Although ...
DOI:
10.1002/jssc.200900554
PubMed:
20024930

44. 2-DE combined with two-layer feature selection accurately establishes the origin of oolong tea

Author:
Han-Ju Chien; Yen-Wei Chu; Chi-Wei Chen; Yu-Min Juang; Min-Wei Chien; Chih-Wei Liu; Chia-Chang Wu; Jason T.C. Tzen; Chien-Chen Lai
Source:
Food chemistry 2016 v.211 pp. 392-399
ISSN:
0308-8146
Subject:
consumer demand; leaves; liquid chromatography; mixing; oolong tea; profits and margins; proteins; support vector machines; tandem mass spectrometry; two-dimensional gel electrophoresis; Taiwan
Abstract:
... Taiwan is known for its high quality oolong tea. Because of high consumer demand, some tea manufactures mix lower quality leaves with genuine Taiwan oolong tea in order to increase profits. Robust scientific methods are, therefore, needed to verify the origin and quality of tea leaves. In this study, we investigated whether two-dimensional gel electrophoresis (2-DE) and nanoscale liquid chromatogr ...
DOI:
10.1016/j.foodchem.2016.05.043

45. 2-Hydroxyterpenylic Acid: An Oxygenated Marker Compound for α-Pinene Secondary Organic Aerosol in Ambient Fine Aerosol

Author:
Kahnt Ariane; Iinuma Yoshiteru; Blockhuys Frank; Mutzel Anke; Vermeylen Reinhilde; Kleindienst Tadeusz E.; Jaoui Mohammed; Offenberg John H.; Lewandowski Michael; Böge Olaf; Herrmann Hartmut; Maenhaut Willy; Claeys Magda
Source:
Environmental Science & Technology 2014 v.48 no.9 pp. 4901-4908
ISSN:
1520-5851
Subject:
acids; aerosols; alpha-pinene; data collection; derivatization; dissociation; gas chromatography; isomers; liquid chromatography; mass spectrometry; photooxidation
Abstract:
... An oxygenated MW 188 compound is commonly observed in substantial abundance in atmospheric aerosol samples and was proposed in previous studies as an α-pinene-related marker compound that is associated with aging processes. Owing to difficulties in producing this compound in sufficient amounts in laboratory studies and the occurrence of isobaric isomers, a complete assignment for individual MW 188 ...
DOI:
10.1021/es500377d

46. 2-O-β-d-Glucopyranosyl l-Ascorbic Acid, a Stable Form of Vitamin C, Is Widespread in Crop Plants

Author:
Alistair T. Richardson; Tony K. McGhie; Sarah B. Cordiner; Teiarere T. H. Stephens; David S. Larsen; William A. Laing; Nigel B. Perry
Source:
Journal of agricultural and food chemistry 2021 v.69 no.3 pp. 966-973
ISSN:
1520-5118
Subject:
Actinidiaceae; Aspleniaceae; Asteraceae; Ericaceae; Malus sylvestris; Piperaceae; Solanaceae; apples; ascorbic acid; bioavailability; crabs; cultivars; ferns and fern allies; food chemistry; liquid chromatography; mass spectrometry; metabolism; seasonal variation
Abstract:
... 2-O-β-d-Glucopyranosyl l-ascorbic acid (AA-2βG) is a stable, bioavailable vitamin C (AA) derivative. We report the distribution and seasonal variation of AA-2βG in apples and its occurrence in other domesticated crops and in wild harvested Ma̅ori foods. Liquid chromatography–mass spectrometry analyses showed high AA-2βG concentrations in crab apples (Malus sylvestris) but low concentrations in dom ...
DOI:
10.1021/acs.jafc.0c06330

47. 2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals

Author:
Ole Lagatie; Emmanuel Njumbe Ediage; Jeroen A. Pikkemaat; Yenny Djuardi; Lieven J. Stuyver
Source:
Parasites & vectors 2017 v.10 no.1 pp. 629
ISSN:
1756-3305
Subject:
Ascaris lumbricoides; biomarkers; clinical trials; liquid chromatography; mass spectrometry; metabolites; nuclear magnetic resonance spectroscopy; urine
Abstract:
... ᅟ: Previous reports suggest that the 2-methyl butyramide and 2-methyl valeramide metabolites of Ascaris lumbricoides in urine of infected individuals could be considered as urinary biomarkers for active infection. We have developed an LC-MS method with a detection limit of 10 ng/mL using synthetic chemicals as reference material. Urine samples (n = 21) of infected individuals were analyzed for the ...
DOI:
10.1186/s13071-017-2600-z
PubMed:
29284514
PubMed Central:
PMC5747143

48. The 2017 fipronil egg contamination incident: The case of Greece

Author:
Chris Anagnostopoulos; George Ampadogiannis; Eleftheria Bempelou; Konstantinos Liapis; Eirini Kastellanou
Source:
Journal of food safety 2020 v.40 no.1 pp. e12727
ISSN:
0149-6085
Subject:
European Union; acaricide residues; calibration; chemical species; cost effectiveness; egg contamination; eggs; electrospray ionization mass spectrometry; farms; fipronil; freezing; liquid chromatography; markets; metabolites; poultry; primary productivity; standard deviation; tandem mass spectrometry; Greece
Abstract:
... In 2017, an outbreak regarding the release to the market of contaminated eggs with fipronil, alerted all EU authorities as to monitor and take relevant measures. In Greece, a total of 40 samples of poultry fat and eggs taken from the primary production (poultry farms) were analyzed, as to investigate the occurrence of fipronil residues. For the analysis of the samples, a simple and cost effective ...
DOI:
10.1111/jfs.12727

49. 202 Proteomic, metabolomic and fatty acid composition in lactating and non-lactating mares’ uterine fluids

Author:
E. Derisoud; A. Hankele; L. Jouneau; C. Dubois; D. Rousseau-Ralliard; M. Dahirel; L. Wimel; S. Ulbrich; P. Chavatte-Palmer
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 229-230
ISSN:
1031-3613
Subject:
algorithms; biosynthesis; computer software; conception; dairy cattle; data collection; databases; diet; embryo (animal); enzymatic hydrolysis; fatty acid composition; foals; gas chromatography; gene expression regulation; genes; guanosine diphosphate; human chorionic gonadotropin; humans; industry; inflammation; lactation; leukotrienes; linear models; liquid chromatography; mammary glands; mannose; mares; metabolites; metabolomics; milk production; ovulation; palmitic acid; peptides; proteasome endopeptidase complex; proteomics; reproductive efficiency; semen; stallions; stearic acid; tandem mass spectrometry; trypsin; ubiquinones; ubiquitin; ultrasonics; uterus
Abstract:
... In the equine industry, horse breeders aim to produce one foal per mare per year. Thus, mares are bred while nursing. In high-yielding dairy cattle, concurrent lactation and conception may affect embryo quality, but effects on uterine fluid (UF) quality are unknown. No data are available in horses. The aim of our study was to analyse the effect of nursing on protein, metabolite and fatty acid (FA) ...
DOI:
10.1071/RDv32n2Ab202

50. 20‐Hydroxyecdysone stimulates nuclear accumulation of BmNep1, a nuclear ribosome biogenesis‐related protein in the silkworm, Bombyx mori

Author:
M.‐M. Ji; A.‐Q. Liu; Y.‐H. Sima; S.‐Q. Xu
Source:
Insect molecular biology 2016 v.25 no.5 pp. 617-628
ISSN:
0962-1075
Subject:
Bombyx mori; animal ovaries; antibodies; biogenesis; ecdysis; ecdysterone; fractionation; gene expression regulation; genes; hormones; immunocytochemistry; immunohistochemistry; instars; invertebrates; liquid chromatography; messenger RNA; metamorphosis; precipitin tests; proteins; ribosomes; silk glands; silkworms; tandem mass spectrometry; vertebrates
Abstract:
... The pathway of communication between endocrine hormones and ribosome biogenesis critical for physiological adaptation is largely unknown. Nucleolar essential protein 1 (Nep1) is an essential gene for ribosome biogenesis and is functionally conserved in many in vertebrate and invertebrate species. In this study, we cloned Bombyx mori Nep1 (BmNep1) due to its high expression in silk glands of silkwo ...
DOI:
10.1111/imb.12248