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- Juan Wang; Qi Gu; Jie Hao; Donghui Bai; Lei Liu; Xiaoyang Zhao; Zhonghua Liu; Liu Wang; Qi Zhou
- Genomics, proteomics & bioinformatics 2013 v.11 pp. 304-311
- umbilical cord, etc ; blood; embryonic stem cells; humans; induced pluripotent stem cells; medicine; neonates; patients; therapeutics; Show all 9 Subjects
- ... Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells ...
- PubMed Central:
- Mellott Adam J.; Godsey Megan E.; Shinogle Heather E.; Moore David S.; Forrest M. Laird; Detamore Michael S.
- Cellular reprogramming 2014 v.16 no.2 pp. 91-97
- umbilical cord, etc ; apoptosis; cell viability; electroporation; embryonic stem cells; freezing; gene expression; green fluorescent protein; humans; induced pluripotent stem cells; medicine; stromal cells; survival rate; tissue engineering; transfection; Show all 15 Subjects
- ... in primary human umbilical cord mesenchymal stromal cells (hUCMSCs) when transfected via Nucleofection™. As hypothesized, the pre-treatment and post-treatment of hUCMSCs transfected via nucleofection with Y-27632-RI significantly improved survival rates of hUCMSCs and gene expression as measured by green fluorescent protein intensity. This study provides the first comparative look at the effect of Y-27632 ...
- PubMed Central:
- Deng Wenwen; Cao Xia; Chen Jingjing; Zhang Zhijian; Yu Qingtong; Wang Yan; Shao Genbao; Zhou Jie; Gao Xiangdong; Yu Jiangnan; Xu Ximing
- ACS Applied Materials & Interfaces 2015 v.7 no.34 pp. 18957-18966
- umbilical cord, etc ; Pleurotus eryngii; genetic engineering; humans; induced pluripotent stem cells; medicine; microRNA; nanoparticles; particle size; physicochemical properties; plasmids; polysaccharides; transcription factors; Show all 13 Subjects
- ... was employed to substitute the oncogenic Klf4 and c-Myc in the OSKM combination as a safer strategy for successful iPSCs generation. The negatively charged plasmid mixture (encoding Oct4, Sox2, miR302-367) and the positively charged cationized Pleurotus eryngii polysaccharide (CPEPS) self-assembled into nanosized particles, named as CPEPS-OS-miR nanoparticles, which were applied to human umbilical cord ...