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You searched for: Subject "umbilical cord" Remove constraint Subject: "umbilical cord" Subject medicine Remove constraint Subject: medicine Subject induced pluripotent stem cells Remove constraint Subject: induced pluripotent stem cells
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1. Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells

Author:
Wang, Juan; Gu, Qi; Hao, Jie; Bai, Donghui; Liu, Lei; Zhao, Xiaoyang; Liu, Zhonghua; Wang, Liu; Zhou, Qi
Source:
Genomics, proteomics & bioinformatics 2013 v.11 pp. 304-311
ISSN:
1672-0229
Subject:
umbilical cord,   etc   ; blood; embryonic stem cells; humans; induced pluripotent stem cells; medicine; neonates; patients; therapeutics;     Show all 9 Subjects
Abstract:
... Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optim ...
DOI:
10.1016/j.gpb.2013.08.002
PubMed:
24080286
PubMed Central:
PMC4357789

2. Improving Viability and Transfection Efficiency with Human Umbilical Cord Wharton's Jelly Cells Through Use of a ROCK Inhibitor

Author:
Mellott, Adam J.; Godsey, Megan E.; Shinogle, Heather E.; Moore, David S.; Forrest, M. Laird; Detamore, Michael S.
Source:
Cellular reprogramming 2014 v.16 no.2 pp. 91-97
ISSN:
2152-4998
Subject:
umbilical cord,   etc   ; apoptosis; cell viability; electroporation; embryonic stem cells; freezing; gene expression; green fluorescent protein; humans; induced pluripotent stem cells; medicine; stromal cells; survival rate; tissue engineering; transfection;     Show all 15 Subjects
Abstract:
... Differentiating stem cells using gene delivery is a key strategy in tissue engineering and regenerative medicine applications. Nonviral gene delivery bypasses several safety concerns associated with viral gene delivery; however, leading nonviral techniques, such as electroporation, subject cells to high stress and can result in poor cell viabilities. Inhibition of Rho-associated coiled-coil kinase ...
DOI:
10.1089/cell.2013.0069
PubMed:
24552552
PubMed Central:
PMC3967373

3. MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via Cationized Pleurotus eryngii Polysaccharide-based Nanotransfection

Author:
Deng, Wenwen; Cao, Xia; Chen, Jingjing; Zhang, Zhijian; Yu, Qingtong; Wang, Yan; Shao, Genbao; Zhou, Jie; Gao, Xiangdong; Yu, Jiangnan; Xu, Ximing
Source:
ACS Applied Materials & Interfaces 2015 v.7 no.34 pp. 18957-18966
ISSN:
1944-8252
Subject:
umbilical cord,   etc   ; Pleurotus eryngii; genetic engineering; humans; induced pluripotent stem cells; medicine; microRNA; nanoparticles; particle size; physicochemical properties; plasmids; polysaccharides; transcription factors;     Show all 13 Subjects
Abstract:
... Induced pluripotent stem cells (iPSCs), resulting from the forced expression of cocktails out of transcription factors, such as Oct4, Sox2, Klf4, and c-Myc (OSKM), has shown tremendous potential in regenerative medicine. Although rapid progress has been made recently in the generation of iPSCs, the safety and efficiency remain key issues for further application. In this work, microRNA 302-367 was ...
DOI:
10.1021/acsami.5b06768