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... Poly(glycolic acid) (PGA) and a series of novel random copolymers of PGA containing 2-hydroxyisobutyrric acid (PGAPHIB) (HIB unit content from 1.5 to 7.4 mol %) were synthesized and characterized in terms of chemical structure and molecular weight. Afterward, the polyesters were examined by thermogravimetric analysis, differential scanning calorimetry, and X-ray diffraction techniques. The copolym ...
... The (co-)pyrolysis of textile dyeing sludge and spent mushroom substrate was conducted to characterize their thermal behaviors and by-products. The devolatilization of textile dyeing sludge mainly occurred between 150 and 500 °C, while the decomposition of inorganic matter as well as the secondary cracking of coke and tar happened between 500 and 1000 °C. The addition of spent mushroom substrate i ...
Retaria; climate change; continental shelf; freshwater; glaciers; isotopes; mass spectrometry; melting; models; oxygen; plankton; radiocarbon dating; sea level; stratigraphy; surface water temperature; New Zealand; Pacific Ocean
Abstract:
... Sedimentary records in shallow-water environments provide a unique opportunity to further our understanding of regional relative sea level changes in relation to global climate change. Here we present a 0.9Ma oxygen isotope stratigraphy for a shallow-water sedimentary transect across three IODP 317 sites in the Canterbury Bight of the southwest Pacific Ocean. The sites, located on the eastern marg ...
... Human diploid cell strains are useful for commercial production of viral vaccines. For example, the MRC-5 cell line (lung fibroblast) is used to produce vaccines against chickenpox and other childhood diseases. To support the safe mass production of such vaccines, it is necessary to cryopreserve standardized batches of concentrated cell suspension, thus ensuring a consistent supply of cells for ma ...
... Towards estimating errors in temperature measurement and control caused by non-collocation of specimen and sensor, we developed a finite-element model of heat transfer in a conduction stage cryomicroscope. The model represented a Linkam Scientific Instruments biological cryostage cooled by a liquid nitrogen pump; finite element analysis was performed using COMSOL Multiphysics® software. The comput ...
... Success rates for cryopreservation of equine sperm show a large variation among individual stallions. Factors that have been implicated with this inter-male variation in cryosurvival are the ability of sperm to tolerate osmotic stress, and temperature-induced changes in membrane phase state and organization. Cells are exposed to osmotic stress both upon the addition of cryoprotective agents as wel ...
... Our laboratory has been investigating the effect of warming rate on survival of mouse oocytes after very rapid cooling. Cooling and warming rates are affected by sample thermal mass, surface area, thermal impedance, and delta T. To maximize these rates we use 0.1 ul samples placed on Cryotops and plunged directly into LN2 for cooling and into room temperature buffer for warming. The maximum warmin ...
chemical composition; electric power; electrons; energy conversion; heat; melting; nuclear magnetic resonance spectroscopy; temperature; topology
Abstract:
... Bi₂Te₃ is a well-known thermoelectric material and, as a new form of quantum matter, a topological insulator. Variation of local chemical composition in Bi₂Te₃ results in formation of several types of atomic defects, including Bi and Te vacancies and Bi and Te antisite defects; these defects can strongly affect material functionality via generation of free electrons and/or holes. Nonuniform distri ...
... Controlling ice growth is a significant tool in many fields such as preventing frost damage to plants, improving cryo-preservation of cells, tissues and organs, the frozen food industry, and cryopreservation procedures. We suggest a control of ice growth in super-cooled solutions by a combination of infrared (IR) radiation and antifreeze materials, such as antifreeze proteins (AFPs), for the purpo ...
... A fundamental strategy evolved by organisms in cold habitats, obliged to cope with ice and freeze injuries, is ice-binding proteins (IBP). These proteins directly interact with ice surfaces and serve to depress the freezing point of the body fluids, to inhibit ice recrystallization, or to promote ice nucleation. The delicate control over ice growth make these proteins applicable in any field that ...
... Lowering the temperature of a biological sample reduces the pace of biochemical metabolism and ultimately stops it altogether. This process, in combination with rewarming the sample with minimal tissue damage, is the essence of cryopreservation. A key obstacle to successful cryopreservation is that, as the biological material is cooled, liquid water within the sample may reorganize into a more sta ...
acetic acid; crystal structure; formic acid; hydrogen bonding; melting; temperature
Abstract:
... The melting diagrams of the binary systems 18-crown-6/formic acid and 18-crown-6/acetic acid have been set up and the crystal structures of the adducts 18C6 · 2 HCOOH (1, space group P 2₁/c with Z = 2 formula units per unit cell) and 18C6 · 2 CH₃COOH (2, C2/m, Z = 2) determined. Furthermore three ternary phases, 18C6 · CH₃COOH · H₂O (3, P2₁/c, Z = 4) and dimorphic 18C6 · 2 CH₃COOH · 4 H₂O (high te ...
... Geoducks are large salt water clams. They are currently farmed in North America. In New Zealand, there is a small wild fishery for the native geoduck, Panopea zelandica. More recently, there is increasing interest in farming this high value species in New Zealand. Already a small batch has been reared and is ready to be experimentally on-grown on existing mussel farms. Cryopreservation can be a po ...
... Collagen, a fibrous structural protein, is a major component of skin, tendon, bone, and other connective tissues. Collagen is one of the dominant biomaterials used for tissue engineering and drug delivery applications. 2,2,2-Trifluoroethanol (TFE) has been used as a co-solvent in the preparation of collagen based biomaterials, which are used for tissue engineering applications. However, the basic ...
cameras; melting; remote sensing; satellites; sea ice; standard deviation; summer; surveys; Arctic region
Abstract:
... A series of shipborne sea ice observations were performed during the Chinese National Arctic Research Expedition in the Pacific Arctic sector between 2 August 2014 and 1 September 2014. Undeformed sea ice thickness (SIT) as well as area fractions of open water, melt pond, and sea ice (Aw, Aₚ, and Aᵢ) were monitored using downward-oriented and oblique-oriented cameras. The results show that SIT var ...
... Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds, such as Citrus, can withstand partial dehydration, but they cannot be stored under conventional genebank conditions because they are cold-sensitive and desiccation does not increase their longevity. Citrus seeds that are immersed in liquid nitrogen (L ...
... Thermodynamics is the study of mathematical relationships arising from physical laws governing energy and entropy. Thermodynamic equilibrium includes thermal equilibrium, mechanical equilibrium and chemical equilibrium. If one of these equilibria is not satisfied in a system, there will be a change towards equilibrium: heat will be transferred, mass will be transferred or change phase (ice will fo ...
... Ice-binding proteins (IBPs) depress the freezing point of the body fluids below the melting point, resulting in a thermal hysteresis (TH) that prevents freezing of the organism in supercooled conditions and inhibits ice re-crystallization in frozen tissues. The potential of these proteins in the medical sector, in cryopreservation, in the frozen food industry, and in agriculture is enormous. The m ...
... The present study aims to estimate the long term changes in sea ice areal extent (SIAE) around Antarctica using Special Sensor Microwave Imager (SSM/I) satellite data (1988–2011). Daily, weekly and monthly brightness temperature (TB) maps of Antarctica have been generated. Temporal variation in monthly averaged TBvalues of sea ice have been analyzed and an increasing trend is observed in TB. Sea i ...
... Drug delivery from 3-dimensional (3D) structures is a rapidly growing area of research. It is essential to achieve structures wherein drug stability is ensured, the drug loading capacity is appropriate and the desired controlled release profile can be attained. Attention must also be paid to the development of appropriate fabrication machinery that allows 3D drug delivery systems (DDS) to be produ ...
... Three-dimensional (3D) printing technologies are emerging as an important tool for the manufacturing of electrodes for various electrochemistry applications. It has been previously shown that metal 3D electrodes, modified with metal oxides, are excellent catalysts for various electrochemical energy and sensing applications. However, the metal 3D printing process, also known as selective laser melt ...
Philip J. Scott; Viswanath Meenakshisundaram; Maruti Hegde; Christopher R. Kasprzak; Christopher R. Winkler; Keyton D. Feller; Christopher B. Williams; Timothy E. Long
... Vat photopolymerization (VP) additive manufacturing fabricates intricate geometries with excellent resolution; however, high molecular weight polymers are not amenable to VP due to concomitant high solution and melt viscosities. Thus, a challenging paradox arises between printability and mechanical performance. This report describes concurrent photopolymer and VP system design to navigate this par ...
electrochemistry; electrodes; energy conversion; manufacturing; melting; models; three-dimensional printing
Abstract:
... Additive manufacturing (also known as three-dimensional (3D) printing) is being extensively utilized in many areas of electrochemistry to produce electrodes and devices, as this technique allows for fast prototyping and is relatively low cost. Furthermore, there is a variety of 3D-printing technologies available, which include fused deposition modeling (FDM), inkjet printing, select laser melting ...
Mammalia; carcinogenesis; digital images; magnetic resonance imaging; melting; rats; salivary glands
Abstract:
... The morphology and the functionality of the murid glandular complex, composed of the submandibular and sublingual salivary glands (SSC), were the object of several studies conducted mainly using magnetic resonance imaging (MRI). Using a 4.7 T scanner and a manganese‐based contrast agent, we improved the signal‐to‐noise ratio of the SSC relating to the surrounding anatomical structures allowing to ...
... Among additive manufacturing (AM) techniques, laser or electron beam based processes have been widely investigated for metallic implants. Despite the potential in manufacturing of patient-specific biomedical implants, 3D inkjet powder printing (3DIJPP, a variant of AM) of biomaterials is still in its infancy, as little is known quantitatively about the transient process physics and dynamics. An eq ...
beeswax; drug carriers; drug formulations; fenofibrate; geometry; manufacturing; medical equipment; melting; models; prediction; solvents; surface area; wettability
Abstract:
... A hot melt 3D inkjet printing method with the potential to manufacture formulations in complex and adaptable geometries for the controlled loading and release of medicines is presented. This first use of a precisely controlled solvent free inkjet printing to produce drug loaded solid dosage forms is demonstrated using a naturally derived FDA approved material (beeswax) as the drug carrier and feno ...
... Three-dimensional (3D) printing is an effective technique for creating variable tactile patterns, such as braille, on planer cellulose paper for visually impaired persons. In this study, we fabricated printed tactile patterns in a size controllable manner with a fused deposition modeling 3D printer. It was demonstrated that the printed dots were adjusted according to size, thickness, shape as well ...
Vipul Gupta; Mohammad Talebi; Jeremy Deverell; Sara Sandron; Pavel N. Nesterenko; Brendan Heery; Fletcher Thompson; Stephen Beirne; Gordon G. Wallace; Brett Paull
analytical chemistry; digestion; direct contact; melting; peptides; polymers; proteins; reversed-phase high performance liquid chromatography; reversed-phase liquid chromatography; stainless steel; temperature profiles; thermal conductivity; titanium
Abstract:
... The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9 mm I.D. × 600 mm long) was produced within a 5 × 30 × 30 mm titanium alloy (Ti–6Al–4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monol ...
air; air temperature; chocolate; extrusion; foods; melting; technology
Abstract:
... 3D printing of foods is an emerging technology that makes it possible to produce unique and complex food items. A number of different foods have already been 3D printed, however, better characterisation of fabrication parameters is still necessary to improve quality and efficiency. In this paper, we present details of the construction of a melt extrusion 3D printer based on readily available open ...
... Poly(methyl methacrylate) (PMMA) was added to aluminum/poly(vinylidene fluoride) (Al/PVDF) energetic blends to enhance melt flow rate and adhesion in a fused deposition modeling (FDM) manufacturing scenario. Composites were successfully printed with up to 30 wt% nano-scale Al after PMMA addition. Melt flow rate increased with increasing PMMA content. This resulted in a partially fluorinated binder ...
... Acne is a multifactorial inflammatory skin disease with high prevalence. In this work, the potential of 3D printing to produce flexible personalised-shape anti-acne drug (salicylic acid) loaded devices was demonstrated by two different 3D printing (3DP) technologies: Fused Deposition Modelling (FDM) and stereolithography (SLA). 3D scanning technology was used to obtain a 3D model of a nose adapted ...
... Impregnation time in Resin Transfer Molding (RTM) is directly proportional to resin viscosity and inversely proportional to fabric permeability, limiting the use of high-viscosity resins with conventional reinforcements. In this study, we show how to reduce the impregnation time in RTM by introducing a second solid phase (spacer) in the core of a fabric stack, fabricated by 3D-printing of a polyme ...
... Stearic acid (SA)/fungi-derived carbon (FDC) composite phase change materials (PCM) were fabricated by vacuum impregnation, where three types of FDC (FDC-C, FDC-H, and FDC-K) as carrier were synthesized by diverse synthetic procedures of carbonization. The FDC-K modified by synergistic hydrothermal and KOH-assisted calcination process had a 3D-cellular structure with considerably higher inner surf ...
... Acrylonitrile–butadiene–styrene (ABS) is one of the most important materials utilized by fused deposition modeling (FDM) 3D printing. In this work, we prepared a series of 3D-printable ABS-based materials with improved scratch resistance and balanced mechanical performance via a simple blending strategy. The scratch resistant performance, mechanical strength, melt flow rate, hardness, transmittanc ...
... The reaction of a melt of unsubstituted imidazole with praseodymium metal yields bright green crystals of 3D-[Pr(Im)₃(ImH)]@ImH. Imidazolate ligands coordinate η¹ via both N atoms their 1,3 positioning within the heterocycle being responsible for the connection of praseodymium atoms. A 3-dimensional network is formed with imidazole molecules from the melt intercalated in the crystal structure. The ...
... Semen lyophilisation is an interesting technique that might be a cheap alternative to long-term storage in liquid nitrogen. The first significant result of this method was achieved by Wakayama and Yanagimachi in the 1998 and demonstrated, for the first time, the birth of healthy mouse pups from epididymal freeze-dried (mouse) spermatozoa. The authors follow the lyophilisation technique, commonly u ...
... In comparison to top-down approaches, nanocomposite thin films are more compatible with nanoparticle (NP) chemistry, device integration, and scalable manufacturing. Nanocomposites have long been promised as an ideal option to fabricate metamaterials that harvest the collective properties enabled by ordered 3D NP assemblies. However, most of accessible NP assemblies, governed by their phase diagram ...
funding; finite element analysis; volatilization; vitrification; melting; computer software; thermal conductivity; heat transfer coefficient; polyethylene; ice; mathematical models; equations; heat; temperature; oocytes; vapors; boiling; cryopreservation; nitrogen; cooling; surveys; specific heat; Argentina
Abstract:
... Advances in cryopreservation of gametes indicate that slow cooling protocols will soon be replaced by fast cooling methods. Vitrification, a process in which liquid water is converted into a glass-like amorphous solid without any ice formation, normally requires plunging directly into liquid nitrogen in order to achieve high cooling rates. This leads to strong nitrogen vaporization around the samp ...
DNA; DNA replication; chemical compounds; chemical reactions; melting; nuclear magnetic resonance spectroscopy; reactive oxygen species
Abstract:
... NMR studies, UV-monitored melting experiments, and ab initio calculations show that 5-hydroxyuracil, produced by the oxidative de-amination of cytosines by reactive oxygen species, can form stable base-pairs with dA, dG, dC and dT residues in a DNA duplex, providing a basis for the in-vivo incorporation of 5-hydroxyuracil during DNA replication. ...
... The study of the conformation properties of macromolecules is at the heart of polymer science. Essentially all physical properties of polymers are manifestations of the underlying polymer conformations or otherwise significantly impacted by the conformation properties. In this Perspective, we review some of the key concepts that we have learned over nearly eight decades of the subject and outline ...
... Although somatic cell nuclear transfer (SCNT) is a valuable tool for producing animals for agricultural and research purposes, the resultant mixing of mitochondrial DNA (mtDNA) from the donor cell and recipient oocyte (heteroplasmy) affects embryo development and offspring survival and health. The aim of this study was to determine the effects of depleting donor cells of their mtDNA before SCNT on ...
... Native DNA lesions in general destabilize DNA secondary structures such as duplex and G-quadruplex because they disrupt optimized interactions in DNA defined by nature. In this paper, we report the first example of a native DNA lesion (8-oxo-7,8-dihydrodeoxyadenosine, OxodA) that stabilizes human telomeric G-quadruplex DNA. CD thermal denaturation studies explicitly displayed increased melting tem ...
... Animal species identification has received growing attention, regarding genetic diversity and food traceability. The objective of this study is to apply a universal primer of part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and goat. PCR product size was 512 bp. The PCR product o ...
... This manuscript describes the interaction of 9-O-substituted analogues of the plant alkaloid berberine with single stranded (ss) poly(A). Three new analogues of berberine with aryl/arylalkyl amino carbonyl methyl substituents at the 9-position of the isoquinoline chromophore were evaluated for their binding to ss poly(A) by a wide variety of biophysical techniques. The results indicated that these ...
... A single-tube multiplex real-time PCR targeting the nuclease (nuc) gene and subsequent high-resolution melting analysis (HRMA) were used to identify 13 different Staphylococcus species. The nuc gene was targeted due to its low intraspecies variation and the greater interspecies variation than the 16S rRNA gene in Staphylococcus. We used HRMA software that can store and compare HRMA profiles from d ...
... On the basis of multi-proxy analysis on TOC, TN, C/N, organic δ¹³C and grain size, sediment record from Qinghai Lake provides evidences of stepwise-patterned climatic change since 16 ka BP. Results show that Qinghai Lake underwent six environmental stages. From 16.2 to 14.3 ka BP and from 4.0 to 2.1 ka BP, the organic δ¹³C value was controlled by the concentration of atmospheric CO₂. Relative high ...
... The Greenland Ice Sheet has experienced significant mass loss in recent years. A substantial component of this is attributable to the retreat of marine-terminating outlet glaciers, which lose mass through increases in calving, submarine melting and terrestrial meltwater discharge. In terms of iceberg production, Jakobshavn Isbræ is the largest marine-terminating glacier in Greenland, yet relativel ...
Daniela Loessner; Anja Rockstroh; Ali Shokoohmand; Boris M. Holzapfel; Ferdinand Wagner; Jeremy Baldwin; Melanie Boxberg; Barbara Schmalfeldt; Ernst Lengyel; Judith A. Clements; Dietmar W. Hutmacher
... Peritoneal invasion through the mesothelial cell layer is a hallmark of ovarian cancer metastasis. Using tissue engineering technologies, we recreated an ovarian tumor microenvironment replicating this aspect of disease progression. Ovarian cancer cell-laden hydrogels were combined with mesothelial cell-layered melt electrospun written scaffolds and characterized with proliferation and transcripto ...
... Small molecule ligands which specifically bind and stabilize G-quadruplex structures in telomeric ends inhibit the activity of telomerase enzyme, an important marker for cancer. Understanding of the binding mode of ligand-G quadruplex complex is important for evaluating relative efficacy of anti-tumor drugs. The present study is focused on interaction of anti-tumor drug mitoxantrone (MTX) with tet ...
... High-resolution records of calibrated proxy data for the past millennium are fundamental to place current changes into the context of pre-industrial natural forced and unforced variability. Although the need for regional spatially-explicit comprehensive reconstructions is widely recognized, the proxy data sources are still scarce, particularly for the Southern Hemisphere and especially for South A ...
... We document concentrations of total mercury (THg) in feathers of Peregrine Falcons (Falco peregrinus; hereafter peregrines) collected during autumn migration at South Padre Island, Texas, and Assateague Island, Maryland, from 2009–2015. We detected THg in all sampled fourth primary (p4; range=0.44–37.46 µg/g) and axillary feathers (range=0.09–62.68 µg/g). We found no significant difference in THg ...
... We describe the stabilization of human IgG1 Fc by an engineered interdomain disulfide bond at the C-terminal end of the molecule. Covalently interconnecting the C-termini of the CH₃ domains led to an increase of the melting temperatures by 5.6 and 9.1°C respectively as compared to CH₃ domains in the context of the wild-type Fc. Combined with a recently described additional intradomain disulfide bo ...
... To describe at molecular level a family with pulverulent congenital cataract associated with a CRYGC gene mutation.One family with several affected members with pulverulent congenital cataract and 230 healthy controls were examined. Genomic DNA from leukocytes was isolated to analyze the CRYGA-D cluster, CX46, CX50 and MIP genes through high-resolution melting curve and DNA sequencing.DNA sequenci ...
... A new route to metallic aerogels that bypasses the use of supercritical fluids and handling fragile wet-gel and aerogel precursors is exemplified by the carbothermal synthesis of monolithic Co(0) aerogels from compressed cobaltia xerogel powders coated conformally (cross-linked) at the primary particle level with a carbonizable polyurea. Residual carbon is removed, and carbon-free samples are obta ...
... In situ generation of copolyesters of PET, followed by test tube screening with a textile dyestuff under mild conditions, produces a rank ordering of dye uptakes mimicking that obtained with independently produced copolymers and with (in some cases) textile dyeing of polyester fibers. From these results, we find that PEG is a generally superior diol polyester modifier, that adipate is superior to ...
... The analysis of the thermal behavior of octyl- (OAC) and decylammonium chloride (DAC) has provided relevant new information. OAC shows a polymorphic phase transition that starts at 308 K and is completed at 313 K, between a monoclinic and a tetragonal structure, which belongs to a space group different from what was observed for other alkylammonium chlorides. At the melting temperature (463 K), th ...
... The organogel of (E)-N′-(anthracene-10-ylmethylene)-3,4,5-tris(dodecyloxy)benzohydrazide (I) in methyl cyclohexane having a fibrillar network structure exhibits excellent fluorescence, which decreases sharply with time upon photoirradiation at λ = 365 nm. It has been attributed to the transformation of the E isomer of I to the Z isomer, and the kinetics of E–Z isomerization are compared for the so ...
... A comparative study was performed in this work to investigate the crystal structure, thermal behavior, melting behavior, overall isothermal melt crystallization kinetics, and spherulitic morphology of biodegradable neat poly(ethylene adipate) (PEA) and its novel copolyester poly(ethylene adipate-co-5 mol % ethylene succinate) P(EA-co-5 mol % ES). Both neat PEA and P(EA-co-5 mol % ES) show the same ...
... The aim of this paper was to study the effects of reinforcing low density polyethylene (LDPE) by using bio-fillers (Doum cellulose or Shrimp chitin) on the mechanical properties. Both, Doum cellulose extracted frsom Doum leaves and Shrimp chitin extracted from shrimp co-products were compounded with LPDE without and with compatibilizer. The biocomposites were prepared by melt blending in a twin-sc ...
... Herein, we present a comparative study of the thermophysical properties of two homologous ionic liquids, namely, trimethyl-sulfonium bis[(trifluoromethyl)sulfonyl]imide, [S₁₁₁][TFSI], and trimethyl-ammonium bis[(trifluoromethyl)sulfonyl]imide, [HN₁₁₁][TFSI], and their mixtures with propylene carbonate, acetonitrile, or gamma butyrolactone as a function of temperature and composition. The influence ...
... One of the most common somatic mutations in breast cancer is found in PIK3CA with a prevalence rate of 18–45%. Different variants of this gene are considered as resistance markers for treatment with HER2-targeted medicines. Conventional molecular methods such as Sanger sequencing are not able to detect mutations with low abundance in a mixture of wild-type DNA, especially in the early stages of ca ...
... We present a modified version of the time-marching algorithm (TMA) [van Ruymbeke et al., 2005, 2010] for predicting the linear viscoelasticity of monodisperse symmetric star polymer melts. Several new elements were added to the original TMA model. In particular, the remaining fraction of the initial tube segments is considered as a function of time, while taking into account the past relaxation hi ...
... We compare six lithium potentials by examining their ability to predict coexistence properties and liquid structure using molecular dynamics. All potentials are of the embedded-atom method type. The coexistence properties we focus on are the melting curve, vapor pressure, saturated liquid density, and vapor–liquid surface tension. For each property studied, the simulation results are compared to a ...
... A study was carried out to compare the thermo-physical behaviors of canola–Engkabang fat blends with those of lard (LD). Four blends were prepared by mixing canola oil with Engkabang fat (CaO/EF) in different ratios: EF-1, 75:25; EF-2, 70:30; EF-3, 65:35; EF-4, 60:40. The fat blends and LD were compared in terms of their basic physicochemical parameters, fatty acid and triacylglycerol (TAG) compos ...
... A yarn-mass variation instrument is described that can be used to provide a continuous monitor of yarn uniforn ity directly on a melt-spinning machine. The mass uniformity of melt spun yarns is a parameter of commercial importance. but uniformity has traditionally been measured off line, at low speeds, and on only a small portion of the yarn package with a Uster evenness tester. The on-line instru ...
... High‐resolution melting (HRM) analysis relies on the use of fluorescent dyes, such as LCGreen, ResoLight, and SYTO9, which bind in a saturated manner to the double‐stranded DNAs. These dyes are expensive in use and may not be affordable when dealing with a large quantity of samples. EvaGreen is a much cheaper DNA helix intercalating dye and has been used in quantitative real‐time polymerase chain ...
... Na⁺ ions play important roles on the physical and chemical properties of aluminosilicate glasses. It is known that they strongly modify the network of tetahedral SiO₄ and AlO₄ units, but the microscopic details on how they alter the local structure remain to be fully established. Here we address this issue by performing classical molecular dynamics simulations over a wide range of glasses composit ...
... The grass breeding industry is interested in a fast and cheap method of identifying contamination in seeds of Italian and perennial ryegrass (Lolium perenne L. and L. multiflorum Lam., respectively). This study shows that high-resolution melting curve analysis in combination with an unlabelled probe assay is an effective method of detecting single nucleotide polymorphisms (SNPs) in diverse Italian ...
... The thermal phase transitions of 18 pure triacylglycerides (TAGs) and four fish oils were investigated using differential scanning calorimetry (DSC) and modulated DSC (MDSC) in the temperature range from −150 to 80°C. The dependence between the heat of fusions and the melting temperatures was determined for pure TAGs, and a regression equation was obtained. A liquid fraction in TAG mixtures and fi ...
... Phase transitions were investigated for Atlantic Salmon, Cod, Herring, Mackerel and Rainbow Trout. The onset of melting was found between −33.5°C and −29.3°C. The amount of unfreezable water was calculated by the DSC (differential scanning calorimetry) melting endotherm integration and was in the range between 5.1% and 8.6% for all investigated samples. The low temperature glass transition was det ...
accuracy; design; energy efficiency; geometry; heat; manufacturing; melting; models; paper; process energy; shape; solutions; spatial distribution; volume
Abstract:
... In the present paper a Design for Additive Manufacturing approach is proposed, with the aim to outline a generalized method and define an energy consumption model that allow, during the development of component design, to evaluate the effects of the different solutions on the energy efficiency of the additive process that will build the component. For this purpose the concept of building energy is ...
Jiazi Tan; Lixia Yang; Alan Ann Lerk Ong; Jiahao Shi; Zhensheng Zhong; Mun Leng Lye; Shiyi Liu; Jolanta Lisowiec-Wachnicka; Ryszard Kierzek; Xavier Roca; Gang Chen
Parkinson disease; alternative splicing; cell culture; chromosomes; dementia; enzymes; exons; fluorescence; humans; introns; melting; mutants; neurons; optical traps; point mutation; proteins; small nuclear RNA
Abstract:
... Alternative splicing of MAPT cassette exon 10 produces tau isoforms with four microtubule-binding repeat domains (4R) upon exon inclusion or three repeats (3R) upon exon skipping. In human neurons, deviations from the ∼1:1 physiological 4R:3R ratio lead to frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). Certain FTDP-17-associated mutations affect a regulatory hairpin t ...
DNA; RNA; fluorescence; genes; ligands; melting; melting point; oligonucleotides; patients; protein synthesis; screening; telomeres; temperature
Abstract:
... Some of the most serious diseases are characterized by the presence of a specific secondary structure within DNA or RNA, often in the promoter or the coding region of the responsible gene, that enhances or disrupts expression of the protein. Structural elements that impact cellular function may also be formed in other genomic regions such as telomeres. Compounds that interact with such structural ...
... In recent years, poly(lactic acid) (PLA) foams have drawn considerable attention worldwide due to their environmental friendliness and excellent biocompatibility. However, it was a big challenge to prepare PLA foams with high volume expansion ratio owing to their poor crystallization properties and melt strength. In this work, the random copolymer of ethylene and glycidyl methacrylate as an effici ...
... Species substitution in meat products is a common problem reported worldwide. This type of food fraud is, typically, an intentional act for economic gain, using sources of low‐priced meats in high‐value meat products. Consequences include economic, health, and religious concerns. Highly sensitive and efficient techniques are thus required to detect meat species. This paper describes a method based ...
Mizuhopecten yessoensis; carotenoids; color; genes; genome-wide association study; high performance liquid chromatography; loci; melting; metabolism; scallops; selection methods; shellfish; single nucleotide polymorphism; visual perception
Abstract:
... The shell color polymorphism widely exists in economic shellfish, which not only results in a better visual perception but also shows great value as an economic trait for breeding. Small numbers of reddish-orange shell Yesso scallops, Patinopecten yessoensis, were found in cultured populations compared to the brown majority. In this study, a genome-wide association study was conducted to understan ...
geophysics; melting; oceans; tectonics; temperature
Abstract:
... The lithosphere-asthenosphere boundary divides the rigid lid from the weaker mantle and is fundamental in plate tectonics. However, its depth and defining mechanism are not well known. We analyzed 15 years of global seismic data using P-to-S (Ps) converted phases and imaged an interface that correlates with tectonic environment, varying from 95 ± 4 kilometers beneath Precambrian shields and platfo ...
... A facile and green route to fabricate sustainable and biodegradable tridimensional interconnected hierarchically meso- and macroporous polylactide (PLA) monoliths was developed. The tunable morphologies and controllable pore sizes of the monoliths based on the stereocomplex (sc) crystallization of poly(l-lactide) (PLLA) and poly(d-lactide) (PDLA) during the melt blending process were caused by the ...
... A blended membrane based on poly(oxyphenylene benzimidazole) (PBI) and ethyl cellulose (EC) exhibits heat resistance and good electrochemical performance. The prepared blended polymer gel membranes show no visible dimensional change after being held at 350 °C for 30 min, whereas the polyethylene (PE) separator almost completely melts. In addition to excellent thermal stability, the self-supporting ...
William J. Sutherland; Steven Broad; Stuart H.M. Butchart; Stewart J. Clarke; Alexandra M. Collins; Lynn V. Dicks; Helen Doran; Nafeesa Esmail; Erica Fleishman; Nicola Frost; Kevin J. Gaston; David W. Gibbons; Alice C. Hughes; Zhigang Jiang; Ruth Kelman; Becky LeAnstey; Xavier le Roux; Fiona A. Lickorish; Kathryn A. Monk; Diana Mortimer; James W. Pearce-Higgins; Lloyd S. Peck; Nathalie Pettorelli; Jules Pretty; Colleen L. Seymour; Mark D. Spalding; Jonathan Wentworth; Nancy Ockendon
biodiversity; carbon; funding; ice; insects; issues and policy; land use change; melting; mercury; permafrost; plant breeding; thawing; weather; China
Abstract:
... We present the results of our tenth annual horizon scan. We identified 15 emerging priority topics that may have major positive or negative effects on the future conservation of global biodiversity, but currently have low awareness within the conservation community. We hope to increase research and policy attention on these areas, improving the capacity of the community to mitigate impacts of pote ...
Lee Keunsoo; Shin Jin-Wook; Park Jun-Hwan; Lee Jonghee; Joo Chul Woong; Lee Jeong-Ik; Cho Doo-Hee; Lim Jong Tae; Oh Min-Cheol; Ju Byeong-Kwon; Moon Jaehyun
... We propose and fabricate a random light scattering layer for light extraction in organic light-emitting diodes (OLEDs) with silver nanodots, which were obtained by melting silver nanowires. The OLED with the light scattering layer as an internal light extraction structure was enhanced by 49.1% for the integrated external quantum efficiency (EQE). When a wrinkle structure is simultaneously used for ...
... Determination of the thermal state of the crust is of fundamental importance to understand the feedbacks between tectonics, rheology and metamorphism. The most important parameters controlling the variation of temperature with depth in the crust are the heat flow across the Moho, the thermal conductivity and the vertical distribution of heat-producing elements ²³⁸,²³⁵U, ²³²Th and ⁴⁰K. The Corsica- ...
... A new method is demonstrated for determining activation energies of microcreep in polymeric filaments. The total activation energy is presented by the generalized form of the Arrhenius rate equation k = A exp (-ΔH RT - ΔF w). Force-elongation data, which are used to evaluate ΔH and ΔF, are obtained with a constant elongation-rate machine capable of elongating a filament as slowly as 0.1 micron/min ...
... Temporal trends of three phenoxy-herbicides (2,4-D, dichlorprop and MCPA) and bromoxynil were determined in the South Tobacco Creek, which drains a 70 km² agricultural watershed near Miami, Manitoba, over a 4-yr sampling program. The quantities of all pesticides used in the watershed and their application times were accurately recorded with the cooperation of landowners. Herbicide concentrations i ...
... We have extended a recently developed multichain slip-spring approach to polymer solutions. The method is based on the dissipative particle dynamics (DPD). Entanglements are mimicked by the inclusion of slip-springs that connect polymer beads, slide along their contour, and are created/destroyed at chain ends. The required average number of slip-springs in polymer melts can be adjusted by the chem ...
... Herein, three-dimensional (3D) N-doped reduced graphene oxide (N-rGO) nanosheets were decorated with a uniform distribution of Co–Ni–S (CNS) nanoparticles to form the CNS/N-rGO composite as a sulfur host material for lithium–sulfur batteries. The CNS nanoparticles and N in CNS/N-rGO strongly interact with polysulfides, whereas graphene, as a conductive network, can improve its electrical conductiv ...
... NLRC (the nucleotide-oligomerization domain (NOD)-like receptor subfamily C) consists of teleost-specific NLRs (NOD-like receptors) and plays pivotal roles in microbial recognition and regulation of innate immune response. In this study, we cloned and characterized a NLRC3-like gene (MamNLRC3-like) from blunt snout bream (Megalobrama amblycephala) by using the quantitative real-time PCR method, an ...
... Advances in real-time polymerase chain reaction (PCR), as well as the emergence of digital PCR (dPCR) and useful modified nucleotide chemistries, including locked nucleic acids (LNAs), have created the potential to improve and expand clinical applications of PCR through their ability to better quantify and differentiate amplification products, but fully realizing this potential will require robust ...
Due to their high crystallinity and aspect ratio, bacterial cellulose nanowhiskers (BCNW) represent an appealing choice for the development of fully biobased nanocomposite materials with high barrier performance. However, their strong tendency to self-associate, together with their highly hydrophilic character, has restricted the production of nanocomposites containing cellulose nanowhiskers by ...
... A new Nearctic species of the fungus gnat genus Palaeodocosia Meunier was discovered in western Michigan. Two adult males flew to a Malaise trap in jack pine (Pinus banksiana Lambert) forest in early December, after the first snows of late autumn had melted. This brings the total number of known extant Palaedocosia species to four. Two are Palaearctic [Palaeodocosia alpicola (Strobl) and Palaeodoc ...
... The predictions of the crystallization temperature and the amount of precipitates of paraffin waxes at high pressure conditions may be inaccurate using existing thermodynamic models. This is mainly due to the lack of experimental data on the molar volume of solid paraffins at high pressures. This inaccuracy is even more pronounced for mixtures of high asymmetry. The present work provides a new acc ...
... Preliminary work has shown the feasibility of a novel method of sizing wool yarns. The method is a nonaqueous, package-to-package technique in which a polymer size is applied to a yarn running through an air fluidized bed of powdered polymer, followed by in situ melting and air cooling. Some water soluble polymers that have properties suitable for application by this method have been investigated. ...
... Graphene was prepared by low temperature vacuum-assisted thermal exfoliation of graphite oxide. The resulting thermally reduced graphene oxide (TRGO) had a specific surface area of 586 m²/g and consisted of a mixture of single-layered and multilayered graphene. The TRGO was added to maleated linear low-density polyethylene LLDPE and to its derivatives with pyridine aromatic groups by melt compound ...
... A novel bio-based polyaspartic acid copolymer, as a multifunctional polymer was fabricated via in-site melting polymerization method using L-aspartic acid, γ-aminobutyric acid, L-phenylalanine and L-alanine as raw materials. The synthetic conditions were studied in detail, and the structure and degradation assays of these copolymers were investigated by FT-IR, XRD, weight loss rate, pH values, int ...
... Poly (L: -aspartic acid-citric acid) green copolymers were developed using thermal polymerization of aspartic acid (ASP) and citric acid (CA) followed by direct bulk melt condensation technique. Antibacterial properties of copolymer of aspartic acid based were investigated as a function of citric acid content. This study is focused on the microorganism inhibition performance of aspartic acid based ...
Bjarke Høgsaa; Ellie H. Fini; Jesper de Claville Christiansen; Albert Hung; Masoumeh Mousavi; Erik Appel Jensen; Farideh Pahlavan; Thomas H. Pedersen; Catalina-Gabriela Sanporean
... Despite the improved thermal stability and mechanical properties of polymer–clay nanocomposites compared to pure polymeric materials, creating nanocomposites with enhanced thermomechanical properties requires a good compatibility and dispersion of the clay within the polymeric matrix. This paper introduces a bioresidue extracted from waste biomass to modify montmorillonite clay to compatibilize it ...
... A new synthetic approach for the low- and mixed-valence double titanium phosphates starting with titanium nitride, TiN, and based on the controlled oxidation of a low-valent metal in the phosphate melt is presented. The reaction of the system Mᴵ₂O - P₂O₅ - TiN (Mᴵ = Na, K) in air (800-1050 °C) leads to loss of nitrogen, N₂, and results in the formation of a number of double phosphates: KTiP₂O₇, K₂ ...
... This work provides a new method to prepare poly(vinyl alcohol) (PVA) hydrogel. Compared with the traditional repeated freeze–thaw method, the physical cross-linking method was adopted to prepare a high-strength hydrogel in one step. The morphology, melting, and crystallization behavior and mechanical properties of the hydrogel were investigated. The hydrogel has a high water content and reswelling ...
... The recovery of materials from urban waste has become progressively more important with wastes providing a variety of resources. This study focuses on the recycling of electronic printed circuit boards (PCBs) from mobile phones, computers, TVs, white goods, and micro-processors etc. that contain significant amounts of hazardous/toxic components along with a variety of metals, ceramics and polymers ...
ammonia; mineral fertilizers; nitrogen; polymerization; volatilization; soil; urea formaldehyde; urea; ammonium; hydrolysis; melting; urease; chemical structure
Abstract:
... Nitrogen loss through NH₃ volatilization is a primary concern for urea as fertilizer due its fast hydrolysis by soil urease. To minimize this problem herein we developed a partially-polymerized urea–formaldehyde granule as a slow-release fertilizer, by melt stage process as a viable route for large-scale production. In this product the unreacted urea fraction acts as a fast release nutrient source ...