Subject: 3 selected / Text Availability: Citation in PubAg

1. Heme oxygenase 1 plays a crucial role in swamp eel response to oxidative stress induced by cadmium exposure or Aeromonas hydrophila infection

Author:: Zang, Yuwei; Zheng, Shuting; Tang, Fang; Yang, Long; Wei, Xiping; Kong, Dan; Sun, Wenxiu; Li, Wei
Source:: Fish physiology and biochemistry 2020 v.46 no.6 pp. 1947-1963
ISSN:: 0920-1742
Subject:: Aeromonas hydrophila; Escherichia coli; Monopterus albus; adverse effects; amino acid sequences; antioxidant activity; bacterial infections; cadmium; cadmium chloride; complementary DNA; fish; gene expression; genes; genetic vectors; heavy metals; heme oxygenase (biliverdin-producing); hepcidin; hosts; interleukin-1beta; intraperitoneal injection; kidneys; liver; messenger RNA; nucleotide sequences; oxidative stress; protective effect; sequence analysis; spleen; stress response; stress tolerance; survival rate; tissues; viability; virulent strains
Abstract:: ... Oxidative stress contributes a lot to initiation and progression of pathological conditions. Heme oxygenase 1 (HO1), a cytoprotective enzyme, is usually upregulated to alleviate oxidative stress in vivo. The function of teleost HO1 in the response to oxidative stress induced by heavy metal exposure and in pathogenic bacterial infection remains uncertain. In the present study, both complementary DN ...
DOI:: 10.1007/s10695-020-00846-0
: http://dx.doi.org/10.1007/s10695-020-00846-0

2. Effects of dietary pyrroloquinoline quinone disodium supplementation on inflammatory responses, oxidative stress, and intestinal morphology in broiler chickens challenged with lipopolysaccharide

Author:: Zheng, Y.W.; Zhang, J.Y.; Zhou, H.B.; Guo, Y.P.; Ma, Q.G.; Ji, C.; Zhao, L.H.
Source:: Poultry science 2020 v.99 no.11 pp. 5389-5398
ISSN:: 0032-5791
Subject:: Escherichia coli; blood serum; body weight; broiler chickens; cages; catalase; duodenum; enzyme activity; feed additives; gene expression; glutathione peroxidase; inflammation; interleukin-10; interleukin-1beta; interleukin-6; lipopolysaccharides; liver; messenger RNA; oxidants; oxidative stress; poultry production; protective effect; quinones; superoxide dismutase; villi
Abstract:: ... This study was conducted to investigate the effects of pyrroloquinoline quinone disodium (PQQ·Na₂) on inflammatory responses, oxidative stress, and intestinal morphology of broiler chickens challenged with lipopolysaccharide (LPS). A 2 × 2 factorial arrangement in a complete randomized design experiment was used to study the effect of dietary PQQ·Na₂ (0 or 1 mg/kg) on broiler chickens with or with ...
DOI:: 10.1016/j.psj.2020.08.007
: https://dx.doi.org/10.1016/j.psj.2020.08.007

3. Effects of lipopolysaccharide exposure in primary bovine ruminal epithelial cells

Author:: Kent-Dennis, C.; Aschenbach, J.R.; Griebel, P.J.; Penner, G.B.
Source:: Journal of dairy science 2020 v.103 no.10 pp. 9587-9603
ISSN:: 0022-0302
Subject:: Escherichia coli; Holstein; Toll-like receptor 2; Toll-like receptor 4; beef cattle; bulls; calves; cell viability; chemokine CXCL2; endotoxins; epithelial cells; flow cytometry; gene expression; gene expression regulation; genes; inflammation; interleukin-7; interleukin-8; lipopolysaccharides; messenger RNA; propidium; quantitative polymerase chain reaction; transcription (genetics); tumor necrosis factors; yearlings
Abstract:: ... The objective of this study was to investigate whether cultured ruminal epithelial cells (REC) responded to lipopolysaccharide (LPS) stimulation and determine whether LPS induced a proinflammatory response. Primary bovine REC were isolated and grown in culture for 2 studies. In study 1, REC were isolated from Holstein bull calves (n = 8) and grown in culture for 10 to 12 d. Cells were then exposed ...
DOI:: 10.3168/jds.2020-18652
: https://dx.doi.org/10.3168/jds.2020-18652

4. Potential Probiotic Lactobacillus rhamnosus (MTCC-5897) Inhibits Escherichia coli Impaired Intestinal Barrier Function by Modulating the Host Tight Junction Gene Response

Author:: Bhat, Mohd Iqbal; Sowmya, Kandukuri; Kapila, Suman; Kapila, Rajeev
Source:: Probiotics and antimicrobial proteins 2020 v.12 no.3 pp. 1149-1160
ISSN:: 1867-1306
Subject:: Escherichia coli; Lactobacillus rhamnosus; food additives; foodborne illness; gastrointestinal diseases; gene expression; genes; human cell lines; intestines; messenger RNA; occludins; phenolsulfonphthalein; probiotics; tight junctions
Abstract:: ... Probiotic as a preventive medicine is emerging as an indispensable tool in addressing the foodborne infections or gastrointestinal disorders. The present study was sought to determine the in vitro prophylactic potential of probiotic Lactobacillus rhamnosus (LR: MTCC-5897) against Escherichia coli (ATCC 14948) induced impairment in intestinal barrier function using Caco-2 cells. Intestinal cells ex ...
DOI:: 10.1007/s12602-019-09608-8
: http://dx.doi.org/10.1007/s12602-019-09608-8

5. Molecular characterization and biological function of a tandem-repeat galectin-9 in Qihe crucian carp Carassius auratus

Author:: Wang, Li; Zhang, Jie; Zhao, Xianliang; Pei, Chao; Li, Li; Kong, Xianghui
Source:: Fish & shellfish immunology 2020 v.103 pp. 366-376
ISSN:: 1050-4648
Subject:: Aeromonas hydrophila; Carassius auratus; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; amino acids; beta-glucans; binding capacity; complementary DNA; erythrocytes; fungi; galectins; gene expression; immune response; kidneys; lipopolysaccharides; liver; messenger RNA; open reading frames; peptidoglycans; quantitative polymerase chain reaction; rabbits; reverse transcriptase polymerase chain reaction; spleen; tissues
Abstract:: ... Galectin-9, as one of the important PRRs in host, could initiate the immune defense responses through recognizing and binding PAMPs on the surface of invading microorganisms. In this study, a new galectin-9 cDNA was identified and characterized in Qihe crucian carp Carassius auratus (named as CaGal-9). The complete cDNA sequence of CaGal-9 was 1318 bp, with an open reading frame (ORF) of 963 bp en ...
DOI:: 10.1016/j.fsi.2020.04.054
: https://dx.doi.org/10.1016/j.fsi.2020.04.054

6. Short communication: Effects of moringa extract on adhesion and invasion of Escherichia coli O55 in bovine mammary epithelial cells

Author:: Cheng, W.N.; Jeong, C.H.; Kim, D.H.; Han, S.G.
Source:: Journal of dairy science 2020 v.103 no.8 pp. 7416-7424
ISSN:: 0022-0302
Subject:: Enterococcus faecalis; Escherichia coli; Moringa; Serratia liquefasciens; Staphylococcus simulans; adhesion; antibacterial properties; antioxidant activity; antioxidant enzymes; bacterial adhesion; cattle; epithelial cells; gene expression; heme oxygenase (biliverdin-producing); in vivo studies; leaf extracts; leaves; mammary glands; messenger RNA; minimum inhibitory concentration; pathogens; quantitative polymerase chain reaction
Abstract:: ... The objective of this study was to evaluate the antibacterial activities of extract derived from moringa leaves. In particular, the effect of moringa extract (Mor) on adhesion and invasion of Escherichia coli O55, Enterococcus faecalis, Staphylococcus simulans, and Serratia liquefaciens was evaluated in bovine mammary epithelial cells (MAC-T). Broth microdilution method, minimum inhibitory concent ...
DOI:: 10.3168/jds.2019-17774
: https://dx.doi.org/10.3168/jds.2019-17774

7. Effects of spermine on ileal physical barrier, antioxidant capacity, metabolic profile and large intestinal bacteria in piglets

Author:: Liu, Guangmang; Mo, Weiwei; Cao, Wei; Wu, Xianjian; Jia, Gang; Zhao, Hua; Chen, Xiaoling; Wu, Caimei; Wang, Jing
Source:: RSC advances 2020 v.10 no.45 pp. 26709-26716
ISSN:: 2046-2069
Subject:: Bifidobacterium; Escherichia coli; Lactobacillus; absorption barrier; alanine; antioxidant activity; antioxidants; bacteria; catalase; cecum; choline; creatine; digesta; digestive enzymes; enzyme activity; gene expression; glutamic acid; glutathione; glutathione peroxidase; glutathione transferase; homeostasis; ileum; immunity; intestinal microorganisms; lactic acid; lysine; messenger RNA; milk; myosin light chain kinase; occludins; phenylalanine; piglets; serine; spermine; threonine; tissues; tyrosine
Abstract:: ... Spermine, a polyamine, exerts important roles in alleviating oxidative damage, improving immunity, increasing antioxidant status and digestive enzyme activities, and promoting the development of small intestine. However, information is not available regarding the effects of spermine supplementation on gut barrier function, intestinal microbiota and metabolic profile in piglets. Therefore, this stu ...
DOI:: 10.1039/c9ra10406b
: http://dx.doi.org/10.1039/c9ra10406b

8. Alterations of peripheral gene expression in response to lipopolysaccharide-induced synovitis as a model for inflammation in horses

Author:: Page, Allen E.; Adam, Emma; Stewart, John C.; Gonzales, Christopher; Barker, Virginia; Horohov, David W.
Source:: Veterinary immunology and immunopathology 2020 v.225 pp. 110058
ISSN:: 0165-2427
Subject:: Escherichia coli; Toll-like receptor 4; amyloid; analysis of variance; blood serum; chemokine CCL8; detection limit; gene expression; gene expression regulation; genes; horses; inflammation; interleukin-10; interleukin-1beta; interleukin-8; lameness; lipopolysaccharides; messenger RNA; mixed breeds; models; synovitis; transcription (genetics); tumor necrosis factor-alpha
Abstract:: ... While the use of lipopolysaccharide (LPS) to induce inflammation has been well described in the horse, the object of this study was to evaluate the effect of repeated intra-articular LPS injections and determine whether this method may be of use to assess changes in gene expression related to inflammation. Six mixed breed horses were utilized for this study, with three horses aged 10−17 years (old ...
DOI:: 10.1016/j.vetimm.2020.110058
: https://dx.doi.org/10.1016/j.vetimm.2020.110058

9. Molecular cloning and characterization of a cathepsin L-like cysteine protease of Angiostrongylus cantonensis

Author:: Bai, Huifang; Cao, Yizhen; Chen, Yunqiu; Zhang, Lingmin; Wu, Chunyun; Zhan, Ximei; Cheng, Mei
Source:: International journal of biological macromolecules 2020 v.153 pp. 1136-1146
ISSN:: 0141-8130
Subject:: Escherichia coli; Parastrongylus cantonensis; adults; amino acids; bioinformatics; cDNA libraries; cathepsin L; complementary DNA; enzyme activity; females; fibrinogen; gelatin; gene expression; genes; heterologous gene expression; humans; immune evasion; immunoglobulin G; messenger RNA; molecular cloning; molecular weight; nutrition; open reading frames; pH; parasites; pathogenesis; sequence alignment; uterus
Abstract:: ... Angiostrongylus cantonensis is a parasitic nematode dwelling in the heart and pulmonary arteries of rats, which can cause angiostrongyliasis in human by accidental infections, manifested as eosinophilic meningitis or meningoencephalitis. Cysteine proteases are the major class of endopeptidases that are expressed at a high level in A. cantonensis, which suggests it may play key roles in pathogenesi ...
DOI:: 10.1016/j.ijbiomac.2019.10.243
: https://dx.doi.org/10.1016/j.ijbiomac.2019.10.243

10. Detection of β-defensins and Other Antibacterial Peptides in P-glycoprotein Expressing Human Gastrointestinal Cell Lines and Their Secretions

Author:: Warrier, A.; Crowe, A.
Source:: Applied biochemistry and microbiology 2020 v.56 no.3 pp. 273-284
ISSN:: 0003-6838
Subject:: Escherichia coli; P-glycoproteins; antimicrobial peptides; antimicrobial properties; bacterial adhesion; gastrointestinal system; gene expression; human cell lines; humans; lipopolysaccharides; messenger RNA; microorganisms; molecular weight; pregnanes; rifampicin; secretion
Abstract:: ... Our laboratory has shown previously [1] that activity of surface P-glycoprotein (P-gp) was inversely associated with bacterial adhesion to the human gastrointestinal cell lines, Caco-2 and LS174T. To attempt a detailed analysis of the rationale for our observations, filtered conditioned medium, that contained only low molecular weight proteins, was taken from these human cell lines with or without ...
DOI:: 10.1134/S0003683820030151
: http://dx.doi.org/10.1134/S0003683820030151

11. A tandem-repeat galectin-1 from Apostichopus japonicus with broad PAMP recognition pattern and antibacterial activity

Author:: Zhang, Chi; Xue, Zhuang; Yu, Zichao; Wang, Hui; Liu, Yu; Li, Huan; Wang, Lingling; Li, Chenghua; Song, Linsheng
Source:: Fish & shellfish immunology 2020 v.99 pp. 167-175
ISSN:: 1050-4648
Subject:: Apostichopus japonicus; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Mesocentrotus; Micrococcus luteus; Paracentrotus lividus; Vibrio splendidus; amino acid sequences; amino acids; antibacterial properties; carbohydrate binding; coelomocytes; digestive system; fish; fungi; galactose; galectins; gene expression; inflammation; integument; invertebrates; lipopolysaccharides; mannose; messenger RNA; muscles; open reading frames; pathogens; peptidoglycans; phylogeny; polypeptides; recombinant proteins; respiratory system
Abstract:: ... Galectins belong to the family of carbohydrate-binding proteins and play major roles in the immune and inflammatory responses of both vertebrates and invertebrates. In the present study, one novel galectin-1 protein named AjGal-1 was identified from Apostichopus japonicas with an open reading frame of 1179 bp encoding a polypeptide of 392 amino acids. The deduced amino acids sequence of AjGal-1 co ...
DOI:: 10.1016/j.fsi.2020.02.011
: https://dx.doi.org/10.1016/j.fsi.2020.02.011

12. Dietary l-arginine supplementation ameliorates inflammatory response and alters gut microbiota composition in broiler chickens infected with Salmonella enterica serovar Typhimurium

Author:: Zhang, Beibei; Li, Guang; Shahid, Muhammad Suhaib; Gan, Liping; Fan, Hao; Lv, Zengpeng; Yan, Shaojia; Guo, Yuming
Source:: Poultry science 2020 v.99 no.4 pp. 1862-1874
ISSN:: 0032-5791
Subject:: C-reactive protein; Escherichia coli; Firmicutes; Methylobacterium; Nitrosomonas; Ochrobactrum intermedium; Rhizobium; Rhodocyclaceae; Rubrobacter xylanophilus; Salmonella Typhimurium; arginine; blood serum; broiler chickens; diamine oxidase; diet; digesta; gene expression; gene expression regulation; histopathology; ileum; inflammation; interferon-gamma; interleukin-10; interleukin-17; interleukin-1beta; interleukin-8; intestinal microorganisms; jejunum; males; messenger RNA; ribosomal DNA; sequence analysis; tumor necrosis factor-alpha
Abstract:: ... This study was conducted to investigate the effects of dietary arginine (Arg) supplementation on the inflammatory response and gut microbiota of broiler chickens subjected to Salmonella enterica serovar Typhimurium. One hundred and forty 1-day-old Arbor Acres male birds were randomly assigned to a 2 × 2 factorial arrangement including diet treatment (with or without 0.3% Arg supplementation) and i ...
DOI:: 10.1016/j.psj.2019.10.049
: https://dx.doi.org/10.1016/j.psj.2019.10.049

13. Progesterone inhibits inflammatory response in E.coli- or LPS-Stimulated bovine endometrial epithelial cells by NF-κB and MAPK pathways

Author:: Cui, Luying; Wang, Heng; Lin, Jiaqi; Wang, Yali; Dong, Junsheng; Li, Jun; Li, Jianji
Source:: Developmental and comparative immunology 2020 v.105 pp. 103568
ISSN:: 0145-305X
Subject:: Escherichia coli; Western blotting; anti-inflammatory activity; bacteria; bacterial infections; cattle; disease susceptibility; endometrium; epithelial cells; fluorescent antibody technique; gene expression; immune response; inflammation; interleukin-1beta; interleukin-6; interleukin-8; lipopolysaccharides; messenger RNA; mitogen-activated protein kinase; phosphorylation; progesterone; quantitative polymerase chain reaction; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:: ... Progesterone suppresses the innate immune function of bovine endometrium, making the uterus susceptible to bacterial infection. The bovine endometrial epithelial cells (BEEC) are the first line of defense against bacteria, such as Escherichia coli (E.coli) that causes inflammation of endometrium through the recognition of lipopolysaccharide (LPS). The aim of this study was to investigate the effec ...
DOI:: 10.1016/j.dci.2019.103568
: https://dx.doi.org/10.1016/j.dci.2019.103568

14. Alteration of insulin signaling to control insect pest by using transformed bacteria expressing dsRNA

Author:: Al Baki, Abdullah; Jung, Jin Kyo; Kim, Yonggyun
Source:: Pest management science 2020 v.76 no.3 pp. 1020-1030
ISSN:: 1526-498X
Subject:: Bacillus thuringiensis; Escherichia coli; Maruca testulalis; RNA interference; Vigna angularis; adults; bacteria; dose response; double-stranded RNA; gene expression; genes; genetic vectors; insect control; insect pests; insecticidal properties; insecticides; insulin; intestines; larvae; larval development; messenger RNA; mortality; oral administration; pupae; reproduction; somatomedins; toxicity
Abstract:: ... BACKGROUND: Insulin/insulin‐like growth factor signaling (IIS) is known to mediate larval growth and adult reproduction in the legume pod borer, Maruca vitrata (Lepidoptera: Crambidae). Four IIS components (InR, FOXO, Akt, and TOR) play crucial roles in the IIS pathway. RESULTS: RNA interference (RNAi) against any of these four IIS component genes was effective in suppressing each target mRNA leve ...
DOI:: 10.1002/ps.5612
: http://dx.doi.org/10.1002/ps.5612

15. Dietary genistein supplementation improves intestinal mucosal barrier function in Escherichia coli O78-challenged broilers

Author:: Zhang, Ming; Kou, Jiao; Wu, Yujun; Wang, Mengmeng; Zhou, Xiumin; Yang, Ying; Wu, Zhenlong
Source:: The Journal of nutritional biochemistry 2020 v.77 pp. 108267
ISSN:: 0955-2863
Subject:: Escherichia coli; Western blotting; apoptosis; average daily gain; birds; blood serum; caspase-3; diet; fluorescein; gene expression; genes; genistein; growth performance; humans; interleukin-6; isothiocyanates; jejunum; males; messenger RNA; mortality; permeability; protective effect; protein content; proteins; secretion; serotypes; sodium chloride; thymus gland; tight junctions; tumor necrosis factor-alpha
Abstract:: ... Genistein has multiple biological activities in both humans and animals. However, a protective effect of genistein on Escherichia coli (E. coli)-induced intestinal mucosal barrier dysfunction remains unknown. In the present study, a total of 288 1-day-old male Arbor Acre broilers fed a corn-soybean basal diet unsupplemented or supplemented with 20 mg genistein/kg diet were subjected to E. coli ser ...
DOI:: 10.1016/j.jnutbio.2019.108267
: https://dx.doi.org/10.1016/j.jnutbio.2019.108267

16. Lso-HPE1, an Effector of ‘Candidatus Liberibacter solanacearum’, Can Repress Plant Immune Response

Author:: Levy, Julien G.; Gross, Rachel; Mendoza-Herrera, Azucena; Tang, Xiaotian; Babilonia, Kevin; Shan, Libo; Kuhl, Joseph C.; Dibble, Margaret S.; Xiao, Fangming; Tamborindeguy, Cecilia
Source:: Phytopathology 2020 v.110 no.3 pp. 648-655
ISSN:: 0031-949X
Subject:: Agrobacterium radiobacter; Apiaceae; Bactericera cockerelli; Candidatus Liberibacter solanacearum; Escherichia coli; Nicotiana benthamiana; alkaline phosphatase; cell death; defense mechanisms; disease control; gene expression; haplotypes; immune response; messenger RNA; plant pathogenic bacteria; proteins; signal peptide; tomatoes; virulence
Abstract:: ... ‘Candidatus Liberibacter solanacearum’ is a plant pathogen affecting the families Solanaceae and Apiaceae in different parts of the world. ‘Ca. L. solanacearum’ is a Gram-negative, fastidious α-proteobacterium that is vectored by different psyllid species. Plant-pathogenic bacteria are known for interfering with the host physiology or defense mechanisms, often by secreting bacterial effectors. Eff ...
DOI:: 10.1094/PHYTO-07-19-0252-R
: http://dx.doi.org/10.1094/PHYTO-07-19-0252-R

17. Safety Assessment of Potential Probiotic Lactobacillus fermentum MTCC-5898 in Murine Model after Repetitive Dose for 28 Days (Sub-Acute Exposure)

Author:: Samtiya, Mrinal; Bhat, Mohd Iqbal; Gupta, Taruna; Kapila, Suman; Kapila, Rajeev
Source:: Probiotics and antimicrobial proteins 2020 v.12 no.1 pp. 259-270
ISSN:: 1867-1306
Subject:: Escherichia coli; Lactobacillus fermentum; adverse effects; alanine transaminase; albino; animal models; aspartate transaminase; blood serum; body weight; cholesterol; creatinine; erythrocyte count; erythrocytes; functional foods; gene expression; glucose; health status; high density lipoprotein; histology; homeostasis; interferon-gamma; interleukin-12; interleukin-4; intestinal mucosa; intestinal secretions; lactate dehydrogenase; leukocytes; lipid composition; liver; low density lipoprotein; messenger RNA; mice; permeability; probiotics; safety assessment; transforming growth factor beta; triacylglycerols; urea; very low density lipoprotein; weanlings
Abstract:: ... Safety assessment of probiotic Lactobacillus fermentum MTCC-5898 (LF) with three doses (10⁷, 10⁹, and 10¹¹ cfu/day/animal) was carried on Swiss albino mouse weanlings for 28 days using oral route. Health status of animals was monitored by physical assessment of body weight, organ indices, and histological appearances of liver and intestine along with measurement of hematological parameters (Hb, WB ...
DOI:: 10.1007/s12602-019-09529-6
: http://dx.doi.org/10.1007/s12602-019-09529-6

18. Exploring bacterial pathogen community dynamics in freshwater beach sediments: A tale of two lakes

Author:: VanMensel, Danielle; Chaganti, Subba Rao; Droppo, Ian G.; Weisener, Christopher G.
Source:: Environmental microbiology 2020 v.22 no.2 pp. 568-583
ISSN:: 1462-2912
Subject:: Escherichia coli; Salmonella; antibiotic resistance; bacterial contamination; beaches; community structure; freshwater; freshwater ecosystems; gene expression; genes; high-throughput nucleotide sequencing; lakes; messenger RNA; microbial communities; microorganisms; models; pathogens; risk; sediment-water interface; sediments; virulence; virulent strains; water quality; Ontario
Abstract:: ... Pathogenic bacteria associated with freshwater ecosystems can pose significant health risks particularly where recreational water use is popular. Common water quality assessments involve quantifying indicator Escherichia coli within the water column but neglect to consider physical and geochemical factors and contributions from the sediment. In this study, we used high‐throughput sequencing to inv ...
DOI:: 10.1111/1462-2920.14860
: http://dx.doi.org/10.1111/1462-2920.14860

19. Meloxicam affects the inflammatory responses of bovine mammary epithelial cells

Author:: Caldeira, M.O.; Bruckmaier, R.M.; Wellnitz, O.
Source:: Journal of dairy science 2019 v.102 no.11 pp. 10277-10290
ISSN:: 0022-0302
Subject:: Escherichia coli; Staphylococcus aureus; amyloid; arachidonic acid; blood serum; bovine mastitis; cell viability; dairy cows; dose response; enzyme inhibition; epithelial cells; gene expression; immune response; immune system; inducible nitric oxide synthase; inflammation; interleukin-8; ligands; lipopolysaccharides; lipoteichoic acids; mammary glands; meloxicam; messenger RNA; nonsteroidal anti-inflammatory agents; pain; pathogen-associated molecular patterns; pathogens; prostaglandin synthase; prostaglandins; serotypes; therapeutics; tumor necrosis factors
Abstract:: ... Nonsteroidal anti-inflammatory drugs are used as supportive therapy with antimicrobial treatments for mastitis in cows to alleviate pain of the inflamed mammary gland. They act mainly by inhibition of cyclooxygenases. Meloxicam (MEL) is a drug designed for cyclooxygenase-2 selectivity, which is upregulated upon inflammation, acting as a key enzyme for the conversion of arachidonic acid to prostagl ...
DOI:: 10.3168/jds.2019-16630
: https://dx.doi.org/10.3168/jds.2019-16630

20. Characterization of a new il-4/13 homologue in grass carp (Ctenopharyngodon idella) and its cooperation with M-CSF to promote macrophage proliferation

Author:: Yang, Kun; Feng, Shiyu; Zhang, Shengnan; Yin, Licheng; Zhou, Hong; Zhang, Anying; Wang, Xinyan
Source:: Fish & shellfish immunology 2019 v.93 pp. 508-516
ISSN:: 1050-4648
Subject:: Ctenopharyngodon idella; Escherichia coli; amino acids; arginase; brain; complementary DNA; enzyme activity; exons; fish; gene expression; gene expression regulation; interferon-gamma; interleukin-4; introns; kidneys; macrophage colony-stimulating factor; macrophages; messenger RNA; monocytes; nitric oxide; nucleotides; phylogeny; sequence alignment
Abstract:: ... In this study, a new il-4/13 cDNA was isolated from grass carp (Ctenopharyngodon idella) using homologous cloning. The phylogenetic tree and sequence alignment of the deduced amino acid (aa) sequence showed that it was closer to grass carp il-4/13b (gcil-4/13b) than other homologues and therefore named gcil-4/13b-like (gcil-4/13bl). It has 399-nt coding sequence (CDS) which is less than gcil-4/13b ...
DOI:: 10.1016/j.fsi.2019.07.070
: https://dx.doi.org/10.1016/j.fsi.2019.07.070

21. Functional analysis and gene expression profiling of extracellular cathepsin Z in red sea bream, Pagrus major

Author:: Choi, Kwang-Min; Joo, Min-Soo; Cho, Dong-Hee; Han, Hyun-Ja; Kim, Myoung Sug; Cho, Mi Young; Jung, Sung Hee; Kim, Do-Hyung; Park, Chan-Il
Source:: Fish & shellfish immunology 2019 v.93 pp. 208-215
ISSN:: 1050-4648
Subject:: Edwardsiella piscicida; Escherichia coli; Iridovirus; Pagrus major; Paralichthys olivaceus; Streptococcus iniae; active sites; amino acid sequences; apoptosis; bacterial infections; cathepsin X; cysteine proteinases; flounder; gene expression; gene expression regulation; genes; glycosylation; homeostasis; immunology; leukocytes; messenger RNA; phylogeny; recombinant proteins; sequence alignment; spleen; tissues; viability
Abstract:: ... Cathepsin Z (CTSZ) is a lysosomal cysteine protease that is known to be involved in the maintenance of homeostasis and the biological mechanisms of immune cells. In this study, we have confirmed the tissue specific expression of the cathepsin Z (PmCTSZ) gene in Pagrus major, and confirmed its biological function after producing recombinant protein using Escherichia coli (E. coli). Multiple sequenc ...
DOI:: 10.1016/j.fsi.2019.07.030
: https://dx.doi.org/10.1016/j.fsi.2019.07.030

22. Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum

Author:: Li, Shengqiang; Ruan, Zeli; Yang, Xiyun; Li, Mingzhu; Yang, Dinglong
Source:: Fish & shellfish immunology 2019 v.93 pp. 66-72
ISSN:: 1050-4648
Subject:: Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Ruditapes philippinarum; Staphylococcus aureus; Vibrio anguillarum; Vibrio harveyi; agglutination; antibacterial properties; biofilm; chemotaxis; chitin; clams; encapsulation; gene expression; gene expression regulation; glucans; hemocytes; hepatopancreas; lectins; lipopolysaccharides; messenger RNA; peptidoglycans; phagocytosis; shellfish; sialic acid; tissues
Abstract:: ... In the present study, a sialic acid-binding lectin was identified and characterized from Manila clam Ruditapes philippinarum (designed as RpSABL-1). Multiple alignments strongly suggested that RpSABL-1 was a new member of the sialic acid-binding lectin family. In non-stimulated clams, RpSABL-1 transcripts were constitutively expressed in all five tested tissues, especially in hepatopancreas. After ...
DOI:: 10.1016/j.fsi.2019.07.027
: https://dx.doi.org/10.1016/j.fsi.2019.07.027

23. Involvement of gamma interferon inducible lysosomal thiol reductase in the innate immune responses of red swamp crayfish, Procambarus clarkii

Author:: Liu, Min; Liu, Li; Abbas, Muhammad Nadeem; Kausar, Saima; Zhang, Jun-Wei; Ye, Zhi-Ze; Qian, Xing-Yi; Zhao, Xiao-Ming; Chu, Sheng-Hui; Dai, Li-Shang
Source:: Developmental and comparative immunology 2019 v.99 pp. 103405
ISSN:: 0145-305X
Subject:: Escherichia coli; Procambarus clarkii; Western blotting; active sites; amino acid sequences; antigens; biotic stress; disulfide bonds; gene expression; gene expression regulation; genes; heart; hepatopancreas; immune response; innate immunity; lipopolysaccharides; mammals; messenger RNA; muscles; oxidoreductases; peptidoglycans; polyacrylamide gel electrophoresis; polyinosinic-polycytidylic acid; proteins; quantitative polymerase chain reaction; sodium dodecyl sulfate; transcription (genetics)
Abstract:: ... The Gamma interferon inducible lysosomal thiol reductase (GILT) plays a key biological role in the immune responses and involves in the processing of class II MHC-restricted antigen by stimulating disulfide bond reduction in mammals. To determine the biological function of GILT in the innate immune system of crustaceans, we sequenced and cloned GILT gene from red swamp crayfish, Procambarus clarki ...
DOI:: 10.1016/j.dci.2019.103405
: https://dx.doi.org/10.1016/j.dci.2019.103405

24. PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL

Author:: Wang, Yun; Yang, Yanyan; Chen, Qianying; Zhai, Hanfei; Xie, Zhaohui; Ke, Fei
Source:: Fish & shellfish immunology 2019 v.93 pp. 567-574
ISSN:: 1050-4648
Subject:: Aeromonas hydrophila; DNA; Escherichia coli; Tachysurus fulvidraco; amino acid sequences; bacterial infections; fish; gene expression; gene expression regulation; immune response; kidneys; leukocytes; lipopolysaccharides; liver; mechanism of action; messenger RNA; nucleoproteins; pathogens; phagocytosis; tissues; tumor necrosis factor-alpha
Abstract:: ... HMGB2, a member of the high mobility group box family, plays an important role in host immune responses. However, the mechanism of action of HMGB2 is not well understood. Herein, a homologue from yellow catfish (Pelteobagrus fulvidraco) was cloned and named PfHMGB2. The deduced amino acid sequence of PfHMGB2 possessed a typical tripartite structure (two DNA binding boxes and an acid tail) and shar ...
DOI:: 10.1016/j.fsi.2019.08.007
: https://dx.doi.org/10.1016/j.fsi.2019.08.007

25. The effect and mechanism of urban fine particulate matter (PM2.5) on horizontal transfer of plasmid-mediated antimicrobial resistance genes

Author:: Xie, Shanshan; Gu, April Z.; Cen, Tianyu; Li, Dan; Chen, Jianmin
Source:: The Science of the total environment 2019 v.683 pp. 116-123
ISSN:: 0048-9697
Subject:: Escherichia coli; air pollution; air quality; antibiotic resistance; antibiotic resistance genes; chemistry; gene expression; membrane permeability; messenger RNA; oxidative stress; particulates; public health; reactive oxygen species; risk; toxicity; China
Abstract:: ... Fine particulate matter (PM2.5) and antimicrobial resistance are two major threats to public health worldwide. Current air pollution studies rely heavily on the assessment of PM2.5 chemistry and toxicity. However, whether and how PM2.5 affects the proliferation and transfer of antimicrobial resistance genes (ARGs) in various environments has remained unanswered. This study investigated the effects ...
DOI:: 10.1016/j.scitotenv.2019.05.115
: https://dx.doi.org/10.1016/j.scitotenv.2019.05.115

26. Effects of Lactococcus lactis MG1363 producing fusion proteins of bovine lactoferricin–lactoferrampin on growth, intestinal morphology and immune function in weaned piglet

Author:: Song, L.; Qiao, X.; Zhao, D.; Xie, W.; Bukhari, S.M.; Meng, Q.; Wang, L.; Cui, W.; Jiang, Y.; Zhou, H.; Li, Y.; Xu, Y.; Tang, L.
Source:: Journal of applied microbiology 2019 v.127 no.3 pp. 856-866
ISSN:: 1364-5072
Subject:: Escherichia coli; Lactobacillus; Lactococcus lactis; Salmonella Enteritidis; Staphylococcus aureus; antibiotics; antimicrobial properties; average daily gain; blood serum; cattle; cecum; diet; endotoxins; feed additives; gene expression; gene expression regulation; growth performance; ileum; immune response; immunoglobulin A; immunoglobulin G; in vitro studies; interleukin-10; interleukin-2; lactic acid; messenger RNA; occludins; oral administration; piglets; probiotics; tight junctions; transforming growth factor beta
Abstract:: ... AIMS: We developed a strategy for localized delivery of the LFCA (lactoferricinlactoferrampin), which is actively synthesized in situ by Lactococcus lactis (pAMJ399‐LFCA/LLMG1363), and explored the possibility of using pAMJ399‐LFCA/LLMG1363 as an alternative additive diet to antibiotics. METHODS AND RESULTS: The antimicrobial activities of the LFCA derived from pAMJ399‐LFCA/LLMG1363 were tested in ...
DOI:: 10.1111/jam.14339
: http://dx.doi.org/10.1111/jam.14339

27. Separation, identification and gene expression analysis of PmAMP-1 from Pinctada fucata martensii

Author:: He, Junjun; Liang, Haiying; Zhu, Jiaping; Fang, Xiaochen
Source:: Fish & shellfish immunology 2019 v.92 pp. 728-735
ISSN:: 1050-4648
Subject:: Escherichia coli; Pinctada fucata; amino acids; antibacterial properties; antimicrobial peptides; bacteria; blood serum; complementary DNA; fungi; gene expression; gills; gonads; hemocytes; hepatopancreas; high performance liquid chromatography; immune response; innate immunity; mass spectrometry; messenger RNA; molecular cloning; muscles; open reading frames; pathogens; proteins; quantitative polymerase chain reaction; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; sequence analysis; shellfish; transmission electron microscopy; viruses
Abstract:: ... Antibacterial peptides (AMPs) constitute an important part of the body's innate immune system and are responsible for a wide range of inhibitory effects against pathogens such as bacteria, fungi, and viruses. In this study, multi-step high performance liquid chromatography (HPLC), combined with Mass Spectrometry (MS), was used to isolate and identify proteins with antibacterial activity from the s ...
DOI:: 10.1016/j.fsi.2019.07.002
: https://dx.doi.org/10.1016/j.fsi.2019.07.002

28. Supplementation exogenous bile acid improved growth and intestinal immune function associated with NF-κB and TOR signalling pathways in on-growing grass carp (Ctenopharyngodon idella): Enhancement the effect of protein-sparing by dietary lipid

Author:: Peng, Xiu-Rong; Feng, Lin; Jiang, Wei-Dan; Wu, Pei; Liu, Yang; Jiang, Jun; Kuang, Sheng-Yao; Tang, Ling; Zhou, Xiao-Qiu
Source:: Fish & shellfish immunology 2019 v.92 pp. 552-569
ISSN:: 1050-4648
Subject:: Aeromonas hydrophila; Bifidobacterium; Ctenopharyngodon idella; Escherichia coli; IKappaB kinase; Lactobacillus; acid phosphatase; animal growth; bacteria; beneficial microorganisms; bile acids; complement; crude protein; dietary fat; enteritis; enzyme activity; feed conversion; feed intake; fish; gene expression; gene expression regulation; growth performance; hepcidin; immune response; immunoglobulin M; interferons; interleukin-10; interleukin-11; interleukin-12; interleukin-15; interleukin-1beta; interleukin-4; interleukin-6; interleukin-8; intestines; lipids; liver; lysozyme; messenger RNA; specific growth rate; target of rapamycin signaling pathways; transcription factor NF-kappa B; transforming growth factor beta 2; tumor necrosis factor-alpha; weight gain
Abstract:: ... This study investigated the effects of bile acid (BA) supplementation on growth performance, intestinal immune function and the mRNA expression of the related signalling molecules in on-growing grass carp (Ctenopharyngodon idella). A total of 540 healthy grass carp (mean weight 179.85 ± 1.34 g) were fed a normal protein and lipid (NPNL) diet containing 29% crude protein (CP) and 5% ether extract ( ...
DOI:: 10.1016/j.fsi.2019.06.047
: https://dx.doi.org/10.1016/j.fsi.2019.06.047

29. Establishment and characterization of two head kidney macrophage cell lines from large yellow croaker (Larimichthys crocea)

Author:: Cui, Kun; Li, Qingfei; Xu, Dan; Zhang, Junzhi; Gao, Shengnan; Xu, Wei; Mai, Kangsen; Ai, Qinghui
Source:: Developmental and comparative immunology 2019
ISSN:: 0145-305X
Subject:: Escherichia coli; Larimichthys crocea; arachidonic acid; cell lines; gene expression; gene expression regulation; immune response; interleukin-1beta; interleukin-8; kidneys; lipopolysaccharides; macrophages; marine fish; messenger RNA; mitogen-activated protein kinase; phagocytosis; respiratory burst; signal transduction; tumor necrosis factor-alpha
Abstract:: ... Two continuous macrophage cell lines (LCM07 and LCM10) were established for the first time from the head kidney of the marine fish large yellow croaker (Larimichthys crocea). To date, both cell lines have been subcultured for more than 100 passages in 12 months. Notably, the LCM07 and LCM10 cells have distinct morphology and immune function. LCM07 cells showed strong contact inhibition in crowded ...
DOI:: 10.1016/j.dci.2019.103477
: https://dx.doi.org/10.1016/j.dci.2019.103477

30. Improved measurement of absolute mRNA quantity without reverse transcription

Author:: Yokomori, Maasa; Tsuge, Kenji; Shohda, Koh-ichiroh; Suyama, Akira
Source:: Analytical biochemistry 2019 v.579 pp. 1-8
ISSN:: 0003-2697
Subject:: Escherichia coli; gene expression; messenger RNA; microarray technology; reverse transcription; transcriptomics
Abstract:: ... Gene expression studies using microarrays have provided important insights into understanding the mechanisms of transcriptional regulation in a variety of biological and disease phenomena. In a previous study, we developed Photo-DEAN, a universal-microarray-based RNA quantification method that enabled reverse transcription-free multiplex measurement of the absolute amount of RNA. Photo-DEAN promot ...
DOI:: 10.1016/j.ab.2019.05.003
: https://dx.doi.org/10.1016/j.ab.2019.05.003

31. Effect of chrysin on changes in intestinal environment and microbiome induced by fructose-feeding in rats

Author:: Andrade, Nelson; Marques, Cláudia; Andrade, Sara; Silva, Cláudia; Rodrigues, Ilda; Guardão, Luísa; Guimarães, João T.; Keating, Elisa; Calhau, Conceição; Martel, Fátima
Source:: Food & function 2019 v.10 no.8 pp. 4566-4576
ISSN:: 2042-650X
Subject:: Bacteroidetes; Escherichia coli; Lactobacillus; adults; chrysin; fructose; gene expression; intestinal microorganisms; jejunum; laboratory animals; males; messenger RNA; metabolic syndrome; microbiome; permeability; polyphenols; rats; tap water; villi
Abstract:: ... Intake of fructose-containing sugars is epidemiological and experimentally linked to metabolic syndrome (MS). We recently verified that the dietary polyphenol chrysin was able to abolish some of the metabolic changes induced by fructose-feeding in the rat. Because the role of the intestine upon fructose-induced MS is poorly understood, we decided to investigate the influence of fructose, in vivo, ...
DOI:: 10.1039/c9fo01142k
: https://dx.doi.org/10.1039/c9fo01142k

32. A superoxide dismutase (MnSOD) with identification and functional characterization from the freshwater mussel Cristaria plicata

Author:: Yang, Wanying; Liu, Wenxiu; Wen, Chungen; Hu, Baoqing; Jian, Shaoqing; Gang, Yang
Source:: Fish & shellfish immunology 2019 v.91 pp. 180-187
ISSN:: 1050-4648
Subject:: Escherichia coli; amino acid sequences; amino acids; complementary DNA; enzyme activity; enzyme stability; freshwater mussels; gene expression; gene expression regulation; gills; hemocytes; hepatopancreas; immunology; manganese; messenger RNA; microcystins; muscles; open reading frames; pH; plasmids; quantitative polymerase chain reaction; recombinant proteins; sequence homology; shellfish; superoxide dismutase; temperature; urea
Abstract:: ... Manganese superoxide dismutase (MnSOD) is a sort of important metalloenzyme that can catalyze ROS in the organisms. In this study, MnSOD cDNA of C. plicata, designated as CpMnSOD (accession no. MK465057), was cloned from hemocytes. The full-length cDNA of MnSOD was 1096 bp with a 672 bp open reading frame encoding 223 amino acids. The deduced amino acid sequence contained a mitochondrial-targeting ...
DOI:: 10.1016/j.fsi.2019.04.307
: https://dx.doi.org/10.1016/j.fsi.2019.04.307

33. Putrescine enhances intestinal immune function and regulates intestinal bacteria in weaning piglets

Author:: Liu, Guangmang; Zheng, Jie; Wu, Xianjian; Xu, Xiaomei; Jia, Gang; Zhao, Hua; Chen, Xiaoling; Wu, Caimei; Tian, Gang; Wang, Jing
Source:: Food & function 2019 v.10 no.7 pp. 4134-4142
ISSN:: 2042-650X
Subject:: Bifidobacterium; Escherichia coli; Lactobacillus; acetates; acid phosphatase; antimicrobial peptides; bacteria; butyrates; castration; cecum; colon; diarrhea; dietary supplements; enzyme activity; gene expression; immune response; immunoglobulin M; inducible nitric oxide synthase; interleukin-6; interleukin-8; intestinal microorganisms; lysozyme; males; messenger RNA; non-specific protein-tyrosine kinase; piglets; propionic acid; putrescine; rapamycin; signal transduction; surface area; transactivators; transcription (genetics); transcription factor NF-kappa B; transforming growth factor beta 1; tumor necrosis factor-alpha; villi; volatile fatty acids; weaning
Abstract:: ... This study aimed to investigate the effect of putrescine on the immune function and intestinal bacteria of weaning piglets. Twenty-four male castrated weaning piglets on their 21st day were randomly assigned into four groups: control (basal diet) and treatment groups given basal diets supplemented with 0.05%, 0.1%, and 0.15% putrescine for 11 days. Results were as follows: (1) Dietary putrescine i ...
DOI:: 10.1039/c9fo00842j
: http://dx.doi.org/10.1039/c9fo00842j

34. Effects of encapsulated organic acids and essential oils on intestinal barrier, microbial count, and bacterial metabolites in broiler chickens

Author:: Yang, Xin; Liu, Yanli; Yan, Fang; Yang, Chengbo; Yang, Xiaojun
Source:: Poultry science 2019 v.98 no.7 pp. 2858-2865
ISSN:: 0032-5791
Subject:: Escherichia coli; Lactobacillus; Toll-like receptor 2; acetic acid; alpha-glucosidase; antibiotics; antimicrobial properties; bacteria; beneficial microorganisms; broiler chickens; broiler feeding; butyric acid; chicks; diet; digesta; digestive enzymes; encapsulation; enzyme activity; essential oils; feed conversion; gene expression; gene expression regulation; ileum; intestinal microorganisms; jejunum; males; messenger RNA; metabolites; mucosa; pH; sucrose alpha-glucosidase; volatile fatty acids
Abstract:: ... The aim of this study was to evaluate a combination diet of organic acids and essential oils on epithelial restitution, intestinal microflora, and volatile fatty acids in broiler chickens. A total of 144 1-day-old male chicks (Cobb 500) were allotted to 3 treatment groups consisting of 6 replicates with 8 birds per replicate. The dietary treatments were as follows: control group (CON, basal diet), ...
DOI:: 10.3382/ps/pez031
: https://dx.doi.org/10.3382/ps/pez031

35. Identification and molecular characterization of a novel anti-lipopolysaccharide factor (ALF) from red swamp crayfish, Procambarus clarkii

Author:: Zhu, Juan-Juan; Ye, Zhi-Ze; Li, Chang-Sheng; Kausar, Saima; Abbas, Muhammad Nadeem; Xiang, Guang-Heng; Qian, Xing-Yi; Dai, Li-Shang
Source:: International journal of biological macromolecules 2019 v.132 pp. 43-50
ISSN:: 0141-8130
Subject:: Escherichia coli; Procambarus clarkii; RNA interference; antibacterial properties; antiviral properties; cysteine; gene expression; genes; hepatopancreas; innate immunity; messenger RNA; nucleotide sequences; pathogens; peptidoglycans; polyclonal antibodies; polyinosinic-polycytidylic acid; quantitative polymerase chain reaction; rabbits; recombinant proteins; reverse transcriptase polymerase chain reaction; signal peptide; tissues
Abstract:: ... Anti-lipopolysaccharide factors are a group of small proteins with broad spectrum antiviral property and antibacterial activity. Herein, we obtained the genomic sequence of the Procambarus clarkii anti-lipopolysaccharide factor (PcALF) gene by using polymerase chain reaction to investigate its expression pattern in various tissues and in the immune tissues (Hepatopancreas) following exposure to pa ...
DOI:: 10.1016/j.ijbiomac.2019.03.167
: https://dx.doi.org/10.1016/j.ijbiomac.2019.03.167

36. Molecular characterization and functional analysis of peroxiredoxin3 cDNA from black tiger shrimp (Penaeus monodon)

Author:: Xie, Dongchang; Wang, Shu; Zhao, Chao; Wang, Pengfei; Qiu, Lihua
Source:: Aquaculture research 2019 v.50 no.7 pp. 1862-1875
ISSN:: 1355-557X
Subject:: Escherichia coli; Penaeus monodon; affinity chromatography; amino acids; antioxidant activity; complementary DNA; dithiothreitol; gene expression; genes; gills; heavy metals; hepatopancreas; hydrogen peroxide; insulin; mechanism of action; messenger RNA; nucleotide sequences; oxidative stress; peroxidase; polypeptides; proteins; reverse transcriptase polymerase chain reaction; tissues; toxicity
Abstract:: ... Peroxidase 3 (Prx‐3) is an important antioxidant protein that protects the body from the toxic effects of reactive oxygen species. In this study, the full‐length Prx3 cDNA sequence (PmPrx3) was isolated from Penaeus monodon. The PmPrx3 cDNA was 942 base pairs long and encoded a 227 amino acid polypeptide. The PmPrx3 protein showed high similarity (74%–83%) with the Prx3 protein from other species ...
DOI:: 10.1111/are.14069
: http://dx.doi.org/10.1111/are.14069

37. Molecular cloning and characterization of SOCS2 from the mealworm beetle Tenebrio molitor

Author:: Kim, Bo Bae; Patnaik, Bharat Bhusan; Jo, Yong Hun; Bang, In Seok
Source:: Entomological research 2019 v.49 no.7 pp. 313-322
ISSN:: 1738-2297
Subject:: Candida albicans; Escherichia coli; Lygus hesperus; Malpighian tubules; Staphylococcus aureus; Tenebrio molitor; Tribolium castaneum; adults; amino acid sequences; amino acids; cytokine receptors; cytokines; eggs; fat body; gene expression; gene expression regulation; hemocytes; innate immunity; insect larvae; insects; messenger RNA; molecular cloning; non-specific protein-tyrosine kinase; open reading frames; phylogeny; polypeptides; signal transduction; transactivators
Abstract:: ... The suppressor of cytokine signaling (SOCS) family of proteins negatively regulate cytokine receptor signaling via the Janus kinase/signal transducer and activator of transcription (STAT) pathway. In this study, we discovered the SOCS2 homolog in the mealworm beetle Tenebrio molitor (TmSOCS2). The full‐length ORF sequence of TmSOCS2 was cloned, and expression pattern in the insect's developmental ...
DOI:: 10.1111/1748-5967.12368
: http://dx.doi.org/10.1111/1748-5967.12368

38. Escherichia coli as a Model for the Description of the Antimicrobial Mechanism of a Cationic Polymer Surface: Cellular Target and Bacterial Contrast Response

Author:: Izzo, Lorella; Matrella, Simona; Mella, Massimo; Benvenuto, Giovanna; Vigliotta, Giovanni
Source:: ACS applied materials & interfaces 2019 v.11 no.17 pp. 15332-15343
ISSN:: 1944-8252
Subject:: Escherichia coli; Gram-positive bacteria; actinomycin D; cell membranes; composite polymers; gene expression; genes; messenger RNA; microbial growth; models; oxidative stress; permeability; phospholipids; polyethylene glycol; protonation; protoplasts; toxicity
Abstract:: ... In this study, we use Escherichia coli as a model to investigate the antimicrobial mechanism of a film made of a copolymer based on monomethylether poly(ethylene glycol), methyl methacrylate, and 2-dimethyl(aminoethyl) methacrylate, whose surface is active towards Gram-negative and Gram-positive bacteria. The polymer contains not quaternized amino groups that can generate a charged surface by prot ...
DOI:: 10.1021/acsami.9b02903
: http://dx.doi.org/10.1021/acsami.9b02903

39. Alteration of the phagocytosis and antimicrobial defense of Octodonta nipae (Coleoptera: Chrysomelidae) pupae to Escherichia coli following parasitism by Tetrastichus brontispae (Hymenoptera: Eulophidae)

Author:: Meng, E.; Li, J.; Tang, B.; Hu, Y.; Qiao, T.; Hou, Y.; Lin, Y.; Li, J.; Chen, Z.
Source:: Bulletin of entomological research 2019 v.109 no.2 pp. 248-256
ISSN:: 1475-2670
Subject:: Chrysomelidae; Escherichia coli; Tetrastichus; animal ovaries; antibacterial properties; antimicrobial peptides; bacterial infections; gene expression; gene expression regulation; hosts; immune response; insects; messenger RNA; parasites; parasitism; pathogens; phagocytosis; pupae
Abstract:: ... Although parasites and microbial pathogens are both detrimental to insects, little information is currently available on the mechanism involved in how parasitized hosts balance their immune responses to defend against microbial infections. We addressed this in the present study by comparing the immune response between unparasitized and parasitized pupae of the chrysomelid beetle, Octodonta nipae ( ...
DOI:: 10.1017/S0007485318000780
PubMed:: 30514411 30514411
: http://dx.doi.org/10.1017/S0007485318000780

40. Characterization of small GTPase Rac1 and its interaction with PAK1 in crayfish Procambarus clarkii

Author:: Feng, Yuanyuan; Ma, Maolin; Zhang, Xiaojiao; Liu, Die; Wang, Lei; Qian, Cen; Wei, Guoqing; Zhu, Baojian
Source:: Fish & shellfish immunology 2019 v.87 pp. 178-183
ISSN:: 1050-4648
Subject:: Escherichia coli; Procambarus clarkii; RNA interference; Western blotting; amino acids; botulinum toxin; cacti and succulents; crayfish; crustin; fish; gene expression; genes; guanosinetriphosphatase; hemocytes; hepatopancreas; innate immunity; lectins; lipopolysaccharides; messenger RNA; open reading frames; polyacrylamide gel electrophoresis; quantitative polymerase chain reaction; sodium dodecyl sulfate; transcription (genetics); tumor necrosis factors
Abstract:: ... Ras-related C3 botulinum toxin substrate 1 (Rac1) participates in many biological processes. In this study, a Rac1 gene was identified in the crayfish Procambarus clarkii with an open reading frame of 579 bp that encoded 192 amino acids. This predicted 21.4 kDa protein was highly homologous to those in other invertebrates. Real-time PCR analysis revealed that Pc-Rac1 was expressed in all examined ...
DOI:: 10.1016/j.fsi.2019.01.013
: https://dx.doi.org/10.1016/j.fsi.2019.01.013

41. Characterization of two copper/zinc superoxide dismutases (Cu/Zn-SODs) from the desert beetle Microdera punctipennis and their activities in protecting E. coli cells against cold

Author:: Xikeranmu, Zilajiguli; Abdunasir, Maimaitiaili; Ma, Ji; Tusong, Kuerban; Liu, Xiaoning
Source:: Cryobiology 2019 v.87 pp. 15-27
ISSN:: 0011-2240
Subject:: Coleoptera; Escherichia coli; Western blotting; bacteria; cold; cold tolerance; cold treatment; gene expression; gene expression regulation; genes; insects; malondialdehyde; messenger RNA; oxidative stress; polyacrylamide gel electrophoresis; proteins; quantitative polymerase chain reaction; superoxide anion; superoxide dismutase; temperature
Abstract:: ... Superoxide dismutases (SODs) are crucial in scavenging reactive oxygen species (ROS); however, studies regarding SOD functions in insects under cold conditions are rare. In this paper, two novel Cu/Zn-SOD genes in the desert beetle Microdera punctipennis, an extracellular copper/zinc SOD (MpecCu/Zn-SOD) and an intracellular copper/zinc SOD (MpicCu/Zn-SOD), were identified and characterized. The re ...
DOI:: 10.1016/j.cryobiol.2019.03.006
: https://dx.doi.org/10.1016/j.cryobiol.2019.03.006

42. Molecular cloning of polymeric immunoglobulin receptor-like (pIgRL) in flounder (Paralichthys olivaceus) and its expression in response to immunization with inactivated Vibrio anguillarum

Author:: Liu, Susu; Du, Yang; Sheng, Xiuzhen; Tang, Xiaoqian; Xing, Jing; Zhan, Webin
Source:: Fish & shellfish immunology 2019 v.87 pp. 524-533
ISSN:: 1050-4648
Subject:: Escherichia coli; Paralichthys olivaceus; Vibrio anguillarum; Western blotting; amino acids; complementary DNA; flounder; gene expression; gills; hindgut; immunization; immunoglobulin M; intraperitoneal injection; kidneys; liquid chromatography; mass spectrometry; messenger RNA; molecular cloning; molecular weight; mucosal immunity; mucus; open reading frames; polyclonal antibodies; polymers; precipitin tests; quantitative polymerase chain reaction; rabbits; receptors; recombinant proteins; skin (animal); spleen; transcription (genetics)
Abstract:: ... In the present work, the polymeric immunoglobulin receptor-like (pIgRL) from flounder (Paralichthys olivaceus) was firstly cloned and identified. The full length cDNA of flounder pIgRL was of 1393 bp including an open reading frame of 1053 bp, and the deduced pIgRL sequence encoded 350 amino acids, with a predicted molecular mass of 39 kDa. There were two immunoglobulin-like domains in flounder pI ...
DOI:: 10.1016/j.fsi.2019.01.039
: https://dx.doi.org/10.1016/j.fsi.2019.01.039

43. The transcription factor NemR is an electrophile-sensing regulator important for the detoxification of reactive electrophiles in Acinetobacter nosocomialis

Author:: Subhadra, Bindu; Surendran, Surya; Kim, Dong Ho; Woo, Kyungho; Oh, Man Hwan; Choi, Chul Hee
Source:: Research in microbiology 2019 v.170 no.3 pp. 123-130
ISSN:: 0923-2508
Subject:: Acinetobacter; Escherichia coli; Lewis acids; biofilm; bioinformatics; cross infection; enzymes; gels; gene expression; messenger RNA; operon; pathogens; promoter regions; quorum sensing; toxicity; transcription factors
Abstract:: ... NemR is an electrophile-sensing regulator which controls two enzymes required for the detoxification of reactive electrophiles: N-ethylmaleimide (NEM) reductase and glyoxalase I in Escherichia coli. Both enzymes are essential for bacterial survival in the presence of toxic reactive electrophiles, such as N-ethylmaleimide and methyl glyoxal. Here, we report the identification and characterization o ...
DOI:: 10.1016/j.resmic.2019.02.001
: https://dx.doi.org/10.1016/j.resmic.2019.02.001

44. Anti-inflammation effects of fucosylated chondroitin sulphate from Acaudina molpadioides by altering gut microbiota in obese mice

Author:: Hu, Shiwei; Wang, Jinhui; Xu, Yangli; Yang, Huicheng; Wang, Jingfeng; Xue, Changhu; Yan, Xiaojun; Su, Laijinn
Source:: Food & function 2019 v.10 no.3 pp. 1736-1746
ISSN:: 2042-650X
Subject:: AMP-activated protein kinase; Acaudina molpadioides; Bacteroidetes; Bifidobacterium; Escherichia coli; Lachnospiraceae; Lactobacillus; Toll-like receptor 4; animal disease models; bacteria; blood serum; chondroitin sulfate; gene expression; inflammation; interleukin-10; intestinal microorganisms; intestines; lipopolysaccharides; messenger RNA; mice; obesity; prebiotics; risk factors; short chain fatty acids
Abstract:: ... This study evaluated the possible prebiotic effects of dietary fucosylated chondroitin sulfate from Acaudina molpadioides (Am-CHS) on the modulation of the gut microbiota and the improvement in the risk factors for chronic inflammation in high fat diet-fed mice. The results showed that the Am-CHS treatment greatly modified the gut microbiota, including the decrease in Bacteroidetes, increase in Fi ...
DOI:: 10.1039/c8fo02364f
: http://dx.doi.org/10.1039/c8fo02364f

45. Eukaryotic Acquisition of a Bacterial Operon

Author:: Kominek, Jacek; Doering, Drew T.; Opulente, Dana A.; Shen, Xing-Xing; Zhou, Xiaofan; DeVirgilio, Jeremy; Hulfachor, Amanda B.; Groenewald, Marizeth; Mcgee, Mcsean A.; Karlen, Steven D.; Kurtzman, Cletus P.; Rokas, Antonis; Hittinger, Chris Todd
Source:: Cell 2019 v.176 no.6 pp. 1356-1366.e10
ISSN:: 0092-8674
Subject:: Escherichia coli; biosynthesis; eukaryotic cells; gene expression; iron; messenger RNA; operon; siderophores; transcription (genetics); yeasts
Abstract:: ... Operons are a hallmark of bacterial genomes, where they allow concerted expression of functionally related genes as single polycistronic transcripts. They are rare in eukaryotes, where each gene usually drives expression of its own independent messenger RNAs. Here, we report the horizontal operon transfer of a siderophore biosynthesis pathway from relatives of Escherichia coli into a group of budd ...
DOI:: 10.1016/j.cell.2019.01.034
CHORUS:: 10.1016/j.cell.2019.01.034
: https://dx.doi.org/10.1016/j.cell.2019.01.034

46. A sigma factor RpoD negatively regulates temperature-dependent metalloprotease expression in a pathogenic Vibrio splendidus

Author:: Zhang, Chi; Zhang, Weiwei; Liang, Weikang; Shao, Yina; Zhao, Xuelin; Li, Chenghua
Source:: Microbial pathogenesis 2019 v.128 pp. 311-316
ISSN:: 0882-4010
Subject:: Apostichopus japonicus; Escherichia coli; Vibrio splendidus; beta-galactosidase; binding sites; enzyme activity; enzyme inhibition; gel electrophoresis; gene expression; genes; messenger RNA; metalloproteinases; pathogens; promoter regions; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sigma factors; temperature; virulence
Abstract:: ... Vibrio splendidus is an important aquatic pathogen that can cause typical symptoms of skin ulceration syndrome (SUS) in sea cucumber Apostichopus japonicus. A metalloprotease Vsm from V. splendidus, has been reported to be an important virulence factor for SUS outbreak. In the present study, the mRNA expression level of vsm was found to correlate to temperature with a peak expression at 28 °C. In ...
DOI:: 10.1016/j.micpath.2019.01.021
: https://dx.doi.org/10.1016/j.micpath.2019.01.021

47. Characterization and in vitro expression of arginine kinase gene in the invasive western flower thrips, Frankliniella occidentalis

Author:: Dong, Fan; Zhang, Nan; Xie, Zhijuan; Meng, Xiangkun; Qian, Kun; Ji, Caihong; Lu, Mingxing; Du, Yuzhou; Wang, Jianjun
Source:: Comparative biochemistry and physiology 2019 v.229 pp. 51-57
ISSN:: 1096-4959
Subject:: Escherichia coli; Frankliniella occidentalis; arginine; arginine kinase; complementary DNA; energy metabolism; enzyme activity; enzyme kinetics; exons; gene expression; genomics; insecticides; insects; introns; messenger RNA; nucleotides; open reading frames; prepupae; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; screening; sequence analysis
Abstract:: ... Arginine kinase (AK) plays a critical role in insect energy metabolism and has been proposed to be a potential insecticide target for commercial exploitation. In this study, the full length cDNA encoding a typical group 1 insect AK (FoAK) was isolated from the western flower thrips (WFT), Frankliniella occidentalis (Pergande). Sequence analysis showed that FoAK contains an open reading frame of 10 ...
DOI:: 10.1016/j.cbpb.2019.01.003
: http://dx.doi.org/10.1016/j.cbpb.2019.01.003

48. Algae-derived β-glucan enhanced gut health and immune responses of weaned pigs experimentally infected with a pathogenic E. coli

Author:: Kim, Kwangwook; Ehrlich, Amy; Perng, Vivian; Chase, Jennifer A.; Raybould, Helen; Li, Xunde; Atwill, Edward R.; Whelan, Rose; Sokale, Adebayo; Liu, Yanhong
Source:: Animal feed science and technology 2019 v.248 pp. 114-125
ISSN:: 0377-8401
Subject:: Escherichia coli; absorption barrier; beta-glucans; blood sampling; blood serum; cortisol; diarrhea; feed supplements; gene expression; gene expression regulation; genes; haptoglobins; heat stability; ileum; immune response; inoculum; interleukin-6; jejunum; messenger RNA; mucosa; neutrophils; occludins; oral administration; permeability; swine; tumor necrosis factor-alpha; yeasts
Abstract:: ... Most of the commercially available β-glucans are derived from yeast, while there are limited research on algae-derived β-glucan in pigs. Therefore, the objective of this experiment was to investigate the influence of dietary supplementation of algae-derived β-glucan on diarrhea, gut permeability, and immune responses of weaned pigs experimentally infected with a pathogenic Escherichia coli (E. col ...
DOI:: 10.1016/j.anifeedsci.2018.12.004
: https://dx.doi.org/10.1016/j.anifeedsci.2018.12.004

49. Identification of porcine CTLA4 gene polymorphism and their association with piglet diarrhea and performance traits

Author:: Gao, Xiaowen; Guo, Dongchun; Kou, Mingxing; Xing, Guiling; Zha, Andong; Yang, Xiuqin; Wang, Xibiao; Di, Shengwei; Cai, Jiancheng; Niu, Buyue
Source:: Molecular biology reports 2019 v.46 no.1 pp. 813-822
ISSN:: 0301-4851
Subject:: Escherichia coli; antigens; average daily gain; cytotoxicity; diarrhea; gene expression; genes; genetic markers; haplotypes; landraces; linkage disequilibrium; messenger RNA; piglets; single nucleotide polymorphism
Abstract:: ... The objective of this study was to evaluate the association between the cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) gene and piglet diarrhea. In this study, the mRNA expression of the CTLA4 gene increased significantly in IPEC-J2 cells after Escherichia coli K88 infection. Single nucleotide polymorphisms (SNPs) located in the 5′ flanking region (SNPs g.107281989C>T) and 3′-untranslated reg ...
DOI:: 10.1007/s11033-018-4536-6
: https://dx.doi.org/10.1007/s11033-018-4536-6

50. CpGSTd3 is a lambda-Cyhalothrin Metabolizing Glutathione S-Transferase from Cydia pomonella (L.)

Author:: Wang, Wei; Hu, Chao; Li, Xin-Ru; Wang, Xiao-Qi; Yang, Xue-Qing
Source:: Journal of agricultural and food chemistry 2019 v.67 no.4 pp. 1165-1172
ISSN:: 1520-5118
Subject:: Cydia pomonella; Escherichia coli; Malpighian tubules; adults; eggs; fruits; gene expression; gene expression regulation; genes; glutathione transferase; inhibitory concentration 50; lambda-cyhalothrin; larvae; messenger RNA; metabolism; midgut; pests; quantitative polymerase chain reaction; quarantine; tissues
Abstract:: ... Little is known about the role of specific delta GST genes in the detoxification of lambda-cyhalothrin in the global quarantine fruit pest codling moth, Cydia pomonella (L.). Real-time quantitative PCR shows that CpGSTd3 was ubiquitously expressed at all developmental stages and is most abundant in the larval stage and lowest in the egg stage; the mRNA level of CpGSTd3 is higher in the midgut and ...
DOI:: 10.1021/acs.jafc.8b05432
: http://dx.doi.org/10.1021/acs.jafc.8b05432

51. Multi-antigenic recombinant subunit vaccine against Lawsonia intracellularis: The etiological agent of porcine proliferative enteropathy

Author:: Montesino, R.; Gutiérrez, N.; Camacho, F.; Farnós, O.; Andrades, S.; González, A.; Acosta, J.; Cortez-San Martín, M.; Sánchez, O.; Ruiz, A; Toledo, J.R.
Source:: Vaccine 2019
ISSN:: 0264-410X
Subject:: Escherichia coli; Lawsonia intracellularis; antibodies; antibody formation; atrophy; bacterial culture; bioinformatics; cell-mediated immunity; cost effectiveness; enzyme-linked immunosorbent assay; epithelial cells; epitopes; etiological agents; gene expression; histopathology; immunization; immunogenicity; immunoglobulin G; interferon-alpha; interleukin-12; intestines; lymphocytes; messenger RNA; mice; outer membrane proteins; porcine proliferative enteritis; pork industry; quantitative polymerase chain reaction; recombinant vaccines; subunit vaccines; swine; villi
Abstract:: ... Proliferative enteropathy, caused by Lawsonia intracellularis, represents a threat for swine industry. Current vaccines are effective but difficult to obtain and scaled up, because of demanding bacterial culture conditions. In this work, a subunit vaccine candidate against L. intracellularis was developed and its efficacy was evaluated in vivo, alone or co-formulated with pig recombinant IFN-α.The ...
DOI:: 10.1016/j.vaccine.2019.01.029
: https://dx.doi.org/10.1016/j.vaccine.2019.01.029

52. Characterization of PGRP-S1 from the oriental armyworm, Mythimna separata

Author:: Yang, Pei-Jin; Zhan, Ming-Yue; Yang, Li-Ling; Liu, Qiong-Qiong; Xu, Yang; Pan, Yue-Min; Rao, Xiang-Jun
Source:: Developmental and comparative immunology 2019 v.90 pp. 121-129
ISSN:: 0145-305X
Subject:: Bacillus subtilis; Escherichia coli; Gram-positive bacteria; Mythimna separata; N-acetylmuramoyl-L-alanine amidase; Staphylococcus aureus; amino acids; cell walls; complementary DNA; fat body; gene expression; hemocytes; immunologic receptors; insects; larvae; messenger RNA; midgut; peptidoglycans; recombinant proteins
Abstract:: ... Peptidoglycan is the key component forming the backbone of bacterial cell wall. It can be recognized by a group of pattern recognition receptors, known as peptidoglycan recognition proteins (PGRPs) in insects and higher animals. PGRPs may serve as immune receptors or N-acetylmuramoyl-L-alanine amidases (EC 3.5.1.28). Here, we report the characterization of a short PGRP, PGRP-S1, from the oriental ...
DOI:: 10.1016/j.dci.2018.09.009
: https://dx.doi.org/10.1016/j.dci.2018.09.009

53. Delineation of the functional and structural properties of the glutathione transferase family from the plant pathogen Erwinia carotovora

Author:: Theoharaki, Christina; Chronopoulou, Evangelia; Vlachakis, Dimitrios; Ataya, Farid S.; Giannopoulos, Panagiotis; Maurikou, Sofia; Skopelitou, Katholiki; Papageorgiou, Anastassios C.; Labrou, Nikolaos E.
Source:: Functional & integrative genomics 2019 v.19 no.1 pp. 1-12
ISSN:: 1438-793X
Subject:: Escherichia coli; Lewis acids; Pectobacterium carotovorum subsp. atrosepticum; abiotic stress; alachlor; biochemical pathways; biodegradation; catalytic activity; chemical treatment; drugs; gene expression; genes; glutathione transferase; isozymes; messenger RNA; phylogeny; plant pathogens; protein synthesis; sequence analysis; toxicity; xenobiotics
Abstract:: ... Erwinia carotovora, a widespread plant pathogen that causes soft rot disease in many plants, is considered a major threat in agriculture. Bacterial glutathione transferases (GSTs) play important roles in a variety of metabolic pathways and processes, such as the biodegradation of xenobiotics, protection against abiotic stress, and resistance against antimicrobial drugs. The GST family of canonical ...
DOI:: 10.1007/s10142-018-0618-8
: http://dx.doi.org/10.1007/s10142-018-0618-8

54. Effects of prophage regions in a plasmid carrying a carbapenemase gene on survival against antibiotic stress

Author:: KIM, So Yeon; KO, Kwan Soo
Source:: International journal of antimicrobial agents 2019 v.53 no.1 pp. 89-94
ISSN:: 0924-8579
Subject:: Escherichia coli; Klebsiella pneumoniae; amikacin; antibiotic resistance; bacteria; beta-lactamase; cefotaxime; gene expression; gene expression regulation; gentamicin; imipenem; knockout mutants; messenger RNA; nucleotide sequences; operon; piperacillin; plasmids; quantitative polymerase chain reaction; reactive oxygen species; sigma factors; survival rate; transcription (genetics); South Korea
Abstract:: ... We investigated the evolutionary importance of cryptic prophage elements in a blaNDM-1-bearing plasmid by exploring the effect of prophage regions on survival against antibiotic stress. While analysing a plasmid harboring an NDM-1-encoding gene in Klebsiella pneumoniae from South Korea, we found a prophage region within the plasmid. We constructed single-prophage knockout (KO) mutants by gene repl ...
DOI:: 10.1016/j.ijantimicag.2018.09.002
: https://dx.doi.org/10.1016/j.ijantimicag.2018.09.002

55. Novel Ca2+-independent C-type lectin involved in immune defense of the razor clam Sinonovacula constricta

Author:: Shi, Yuhong; Zhao, Xuelin; Wang, Zhenhui; Shao, Yina; Zhang, Weiwei; Bao, Yongbo; Li, Chenghua
Source:: Fish & shellfish immunology 2019 v.84 pp. 502-508
ISSN:: 1050-4648
Subject:: Escherichia coli; Gram-negative bacteria; Micrococcus luteus; Veneroida; Vibrio anguillarum; Vibrio parahaemolyticus; agglutination; amino acids; carbohydrates; complementary DNA; gene expression; gene expression regulation; gills; hepatopancreas; innate immunity; lectins; messenger RNA; open reading frames; pathogens; polypeptides; shellfish
Abstract:: ... C-type lectins (CTLs) are important pattern recognition molecules that participate in bacterial binding and agglutination by specific recognition of carbohydrates from pathogens. In this study, a full-length cDNA of CTL was cloned from Sinonovacula constricta (designated ScCTL-2). ScCTL-2 has a length of 981 bp, a 5′-untranslated region (UTR) of 47 bp, a short 3′-UTR of 37 bp, and an open reading ...
DOI:: 10.1016/j.fsi.2018.10.036
: https://dx.doi.org/10.1016/j.fsi.2018.10.036

56. Physiologic and innate immunity responses to bacterial lipopolysaccharide administration in beef heifers supplemented with OmniGen-AF

Author:: Brandão, A. P.; Cooke, R. F.; Schubach, K. M.; Marques, R. S.
Source:: Animal 2019 v.13 no.1 pp. 153-160
ISSN:: 1751-732X
Subject:: Alopecurus pratensis; Escherichia coli; animal health; beef cattle; blood; cortisol; cross-over studies; distillers grains; gene expression; haptoglobins; hay; heifers; immune response; immunocompetence; ingredients; innate immunity; interleukin-8; intravenous injection; ligands; lipopolysaccharides; messenger RNA; neoplasms; prostaglandin synthase; temperature; New Jersey
Abstract:: ... Nutritional alternatives to strengthen animal immunocompetence are critical for welfare and productivity in livestock systems, such as beef cattle operations. This experiment evaluated physiological and innate immunity effects of supplementing an immunomodulatory feed ingredient (Omnigen-AF; Phibro Animal Health, Teaneck, NJ, USA) to beef heifers administered bacterial lipopolysaccharide (LPS). In ...
DOI:: 10.1017/S1751731118001441
PubMed:: 29929569 29929569
: http://dx.doi.org/10.1017/S1751731118001441

57. Molecular characterization and functional analysis of IL-12p40 from Chinese sea bass (Lateolabrax maculatus) under biotic and abiotic stresses

Author:: Chen, Xiang; Wang, Pengfei; Zhao, Chao; Yan, Lulu; Lin, Heizhao; Qiu, Lihua
Source:: Fish & shellfish immunology 2018 v.83 pp. 373-385
ISSN:: 1050-4648
Subject:: Escherichia coli; Lateolabrax japonicus; abiotic stress; amino acids; antimicrobial properties; bass; gene expression; gene expression regulation; growth retardation; immune response; innate immunity; interleukin-12; intestines; kidneys; liquids; liver; messenger RNA; oxidative stress; polypeptides; protein-protein interactions; proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; shellfish; spleen; tissues; toxicity; transcription (genetics)
Abstract:: ... Interleukins are critical cytokines that are ubiquitously present in both vertebrates and invertebrates and constitute the front line of host innate immunity. Here, we identified and analyzed IL-12p40 from the Chinese sea bass Lateolabrax maculatus (LmIL-12p40). The LmIL-12p40 gene is expressed as a 1386-base pair transcript that encodes a polypeptide of 321 amino acids. Transcriptional expression ...
DOI:: 10.1016/j.fsi.2018.09.038
: https://dx.doi.org/10.1016/j.fsi.2018.09.038

58. Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes

Author:: Cho, Hyejin; Kim, Kwang-sun
Source:: Biochemical and biophysical research communications 2018 v.506 no.1 pp. 66-72
ISSN:: 0006-291X
Subject:: Escherichia coli; Oxalis; antibiotic resistance; cephalothin; cyclic AMP; drugs; gene expression; gene expression regulation; genes; genetic markers; medicine; messenger RNA; multiple drug resistance; non-coding RNA; pathogens; phenotype; prediction; sequence analysis; therapeutics; transcription (genetics)
Abstract:: ... Antibiotics have been one of the most successful forms of therapy in medicine. However, the efficiency of antibiotics is compromised by the emergence of antibiotic-resistant pathogens. To reduce antibiotic resistance, complete understanding of bacterial tactics to defend themselves against antibiotics is necessary. Small-noncoding RNAs (sRNAs) modulate gene expression by base-pairing with multiple ...
DOI:: 10.1016/j.bbrc.2018.10.084
: https://dx.doi.org/10.1016/j.bbrc.2018.10.084

59. Avian pathogenic Escherichia coli-induced activation of chicken macrophage HD11 cells

Author:: Peng, Lianci; Matthijs, Mieke G.R.; Haagsman, Henk P.; Veldhuizen, Edwin J.A.
Source:: Developmental and comparative immunology 2018 v.87 pp. 75-83
ISSN:: 0145-305X
Subject:: Escherichia coli; chickens; gene expression; interferon-beta; interleukin-10; interleukin-1beta; interleukin-6; interleukin-8; macrophages; messenger RNA; neutralization; nitric oxide; phagocytosis; respiratory tract diseases; temperature; viability
Abstract:: ... Avian pathogenic Escherichia coli (APEC) can cause severe respiratory diseases in poultry. The initial interaction between APEC and chicken macrophages has not been characterized well and it is unclear how effective chicken macrophages are in neutralizing APEC. Therefore, the effect of APEC on activation of chicken macrophage HD11 cells was studied. Firstly, the effect of temperature (37 vs 41 °C) ...
DOI:: 10.1016/j.dci.2018.05.019
: https://dx.doi.org/10.1016/j.dci.2018.05.019

60. Molecular characterization, expression and immune functions of cystatin B in Japanese flounder (Paralichthys olivaceus)

Author:: Yu, Haiyang; Si, Yu; Liu, Yuxiang; Liu, Jinxiang; Xu, Xin; Zhang, Quanqi; Wang, Xubo
Source:: Fish & shellfish immunology 2018 v.81 pp. 463-469
ISSN:: 1050-4648
Subject:: Escherichia coli; Paralichthys olivaceus; bacteria; cystatins; cysteine proteinases; enzyme inhibition; flounder; gene expression; genes; immune response; interferon-gamma; interleukin-10; interleukin-1beta; lipopolysaccharides; messenger RNA; pathogens; peptidoglycans; polyinosinic-polycytidylic acid; proteins; quantitative polymerase chain reaction; shellfish; tissues; tumor necrosis factor-alpha
Abstract:: ... Cystatin B is an intracellular inhibitor that regulates the activities of cysteine proteases. In this study, cystatin B in Japanese flounder (Paralichthys olivaceus) was characterized and its immune function was analyzed. This gene had a high similarity with the sequence of cystatin B in other fish species, and the derived peptide shared typical features of cystatin proteins including the QXVXG mo ...
DOI:: 10.1016/j.fsi.2018.07.054
: https://dx.doi.org/10.1016/j.fsi.2018.07.054

61. The acute stresses role of the atypical 2-cys peroxiredoxin PmPrx5 in black tiger shrimp (Penaeus monodon) from biological immunity and environmental toxicity stress

Author:: Bu, Ruiqian; Yan, Lulu; Zhao, Chao; Wang, Pengfei; Fan, Sigang; Wang, Shu; Qiu, Lihua
Source:: Fish & shellfish immunology 2018 v.81 pp. 189-203
ISSN:: 1050-4648
Subject:: Escherichia coli; Macrobrachium rosenbergii; Palaemon; Penaeus monodon; acidity; alkalinity; amino acids; antibacterial properties; antioxidant activity; apoptosis; biomarkers; cadmium; complementary DNA; copper; drugs; ecdysis; ecotoxicology; fish; gene expression; gene expression regulation; gills; growth retardation; heavy metals; hepatopancreas; immune response; isoelectric point; liquids; messenger RNA; molecular weight; open reading frames; osmotic pressure; oxidative stress; peroxides; peroxiredoxin; phylogeny; pollutants; proteins; sequence alignment; shellfish; shrimp; signal peptide; therapeutics; tissues; toxicity; virulent strains
Abstract:: ... As a unique atypical 2-Cys Peroxiredoxin (Prx) of the Prx-like superfamily, Peroxiredoxin5 (Prx5) possesses special properties, such as its enzymatic mechanism, wide subcellular distribution and high affinity for peroxides and peroxynitrite. Prx5 plays a crucial role in oxidative stress, immune responses, cell apoptosis, proliferation, differentiation, intracellular signaling, the modulation of ge ...
DOI:: 10.1016/j.fsi.2018.03.042
: https://dx.doi.org/10.1016/j.fsi.2018.03.042

62. Molecular Characterization of a Mitochondrial Manganese Superoxide Dismutase From Chilo suppressalis (Lepidoptera: Crambidae)

Author:: Tu, Xiao-Hui; Zhuo, Yu-Li; Jiang, Xiu-Yun; Liu, Hong-Li; Cao, Ye; Liu, Su
Source:: Journal of economic entomology 2018 v.111 no.5 pp. 2391-2400
ISSN:: 1938-291X
Subject:: Chilo suppressalis; Escherichia coli; abamectin; cadmium chloride; chlorpyrifos; copper sulfate; crops; enzyme activity; gene expression; gene expression regulation; genes; hydrogen peroxide; insect pests; manganese; messenger RNA; mitochondria; oxidative stress; paraquat; peptides; pupae; rice; superoxide dismutase; temperature; thermal stability
Abstract:: ... In insects, superoxide dismutases (SODs) play a critical role in the scavenging of harmful reactive oxygen species (ROS) and protecting against oxidative stress induced by various environmental stresses. The Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is an economically important insect pest of rice crops. In this study, a mitochondrial manganese SOD (Cs-mMnSOD) gene ...
DOI:: 10.1093/jee/toy180
: https://dx.doi.org/10.1093/jee/toy180

63. A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action

Author:: Yang, Dinglong; Zhang, Qianqian; Wang, Qing; Chen, Lizhu; Liu, Yongliang; Cong, Ming; Wu, Huifeng; Li, Fei; Ji, Chenglong; Zhao, Jianmin
Source:: Fish & shellfish immunology 2018 v.80 pp. 274-280
ISSN:: 1050-4648
Subject:: Escherichia coli; Gram-negative bacteria; Ruditapes philippinarum; Vibrio anguillarum; Vibrio splendidus; amino acids; antibacterial properties; antimicrobial peptides; cell death; clams; fish; gene expression; gene expression regulation; hemocytes; hepatopancreas; innate immunity; isoelectric point; mechanism of action; membrane permeability; messenger RNA; molecular weight; open reading frames; phylogeny; polypeptides; proteins; shellfish; synthetic peptides; tissues
Abstract:: ... Defensins are small cysteine-rich cationic proteins that are ubiquitously present in both vertebrates and invertebrates and constitute the front line of host innate immunity. In the present study, a defensin-like antimicrobial peptide (designed as RpdefB) was identified and characterized from the manila clam Ruditapes philippinarum. The open reading frame of RpdefB encoded a 70-amino acid polypept ...
DOI:: 10.1016/j.fsi.2018.06.019
: https://dx.doi.org/10.1016/j.fsi.2018.06.019

64. Identification of two IL-4/13 homologues in large yellow croaker (Larimichthys crocea) revealed their similar roles in inducing alternative activation of monocytes/macrophages

Author:: Mao, Kaiqiong; Chen, Wei; Mu, Yinnan; Ao, Jingqun; Chen, Xinhua
Source:: Fish & shellfish immunology 2018 v.80 pp. 180-190
ISSN:: 1050-4648
Subject:: CD4-positive T-lymphocytes; Dicentrarchus labrax; Escherichia coli; Larimichthys crocea; Takifugu rubripes; amino acid sequences; bacterial vaccines; fish; gene expression; gene expression regulation; genes; inducible nitric oxide synthase; interleukin-13; interleukin-1beta; interleukin-4; kidneys; macrophages; mammals; messenger RNA; monocytes; nitric oxide; phagocytosis; phylogeny; proteins; sequence analysis; shellfish; tissues; transforming growth factor beta; tumor necrosis factor-alpha; vascular endothelial growth factors
Abstract:: ... Mammalian interleukin-4 (IL-4) and −13 (IL-13), two anti-inflammatory T helper cell type 2 (Th2) cytokines, play the central roles in mediating the alternative activation of monocytes/macrophages (MO/Mφs). However, exact functions in MO/Mφs polarization of IL-4/13 homologues in teleost fish remain largely unknown. In this study, we identified two IL-4/13 homologues from large yellow croaker Larimi ...
DOI:: 10.1016/j.fsi.2018.06.002
: https://dx.doi.org/10.1016/j.fsi.2018.06.002

65. Isolation, expression, and functional analysis of developmentally regulated plasma membrane polypeptide 1 (DREPP1) in Sporobolus virginicus grown under alkali salt stress

Author:: Theerawitaya, Cattarin; Yamada-Kato, Nana; Singh, Harminder Pal; Cha-um, Suriyan; Takabe, Teruhiro
Source:: Protoplasma 2018 v.255 no.5 pp. 1423-1432
ISSN:: 0033-183X
Subject:: Arabidopsis thaliana; C4 plants; Escherichia coli; Panicum; Setaria italica; Sporobolus virginicus; amino acids; calcium; calmodulin; gene expression; gene expression regulation; genes; grasses; halophytes; leaves; messenger RNA; microtubules; myristoylation; plasma membrane; polymerization; polypeptides; rice; salt stress; stress response; tissues
Abstract:: ... The plant specific DREPP proteins have been shown to bind Ca²⁺ and regulate the N-myristoylation signaling and microtubule polymerization in Arabidopsis thaliana. The information about DREPP proteins in other plants is, however, scarce. In the present study, we isolated the DREPP gene from a halophytic grass, Sporobolus virginicus, and tested whether the gene was involved in alkaline salt stress r ...
DOI:: 10.1007/s00709-018-1242-0
: https://dx.doi.org/10.1007/s00709-018-1242-0

66. Vitamin E alleviates phoxim-induced toxic effects on intestinal oxidative stress, barrier function, and morphological changes in rats

Author:: Sun, Yuecheng; Zhang, Jing; Song, Wentao; Shan, Anshan
Source:: Environmental science and pollution research international 2018 v.25 no.26 pp. 26682-26692
ISSN:: 0944-1344
Subject:: DNA; Escherichia coli; Lactobacillus; feeds; gene expression; genes; humans; ileum; interleukin-1beta; interleukin-6; interleukin-8; intestinal microorganisms; intestinal mucosa; jejunum; laboratory animals; messenger RNA; organophosphorus pesticides; oxidative stress; phosphorus; phoxim; rats; secretion; superoxide dismutase; toxicity; tumor necrosis factor-alpha; villi; vitamin E
Abstract:: ... Phoxim is an organic phosphorus pesticide that remains easily in the environment, such as human food and animal feed. The objective of this study was to explore the effect of vitamin E on phoxim-induced oxidative stress in the intestinal tissues of Sprague-Dawley (SD) rats. Forty-eight Sprague-Dawley rats were randomly assigned to a control group and three treatment groups: treatment group 1 (phox ...
DOI:: 10.1007/s11356-018-2666-y
: http://dx.doi.org/10.1007/s11356-018-2666-y

67. Molecular characteristics, expression, and antimicrobial activities of i-type lysozyme from the razor clam Sinonovacula constricta

Author:: Chen, Fan; Wei, Zhixin; Zhao, Xuelin; Shao, Yina; Zhang, Weiwei
Source:: Fish & shellfish immunology 2018 v.79 pp. 321-326
ISSN:: 1050-4648
Subject:: Escherichia coli; Veneroida; Vibrio parahaemolyticus; Vibrio splendidus; amino acids; antimicrobial properties; complementary DNA; fish; gene expression; hepatopancreas; innate immunity; lysozyme; messenger RNA; minimum inhibitory concentration; pathogens; phylogeny; polypeptides; recombinant proteins; sequence alignment; shellfish; signal peptide; tissue distribution; tissues
Abstract:: ... Lysozyme is a key component of the innate immune system, which plays a pivotal role in early defense against pathogen infection. In this study, an i-type lysozyme homology was identified from the razor clam Sinonovacula constricta (designated as ScLYZ) through RACE approaches. The full-length cDNA of ScLYZ was 768 bp and encoded a polypeptide of 140 amino acid residues. SMART analysis revealed tha ...
DOI:: 10.1016/j.fsi.2018.05.037
: https://dx.doi.org/10.1016/j.fsi.2018.05.037

68. Identification of agmatinase from Vibrio splendidus and its roles in modulating arginine metabolism of Apostichopus japonicus

Author:: Shao, Yina; Zhang, Shanshan; Li, Chenghua; Zhang, Weiwei; Zhao, Xuelin; Xiong, Jinbo
Source:: Aquaculture 2018 v.492 pp. 1-8
ISSN:: 0044-8486
Subject:: Apostichopus japonicus; Escherichia coli; Vibrio splendidus; agmatine; arginase; arginine; aspartic acid; bacteria; bioactive properties; catalytic activity; enzyme activity; gene expression; guanidinium; histidine; innate immunity; messenger RNA; metabolism; molecular cloning; monitoring; nitric oxide; open reading frames; pathogens; recombinant proteins; sequence alignment; site-directed mutagenesis
Abstract:: ... Innate immunity is the first line of defense against invading pathogens. However, pathogenic bacteria have developed various mechanisms, such as direct competition for common substrates and transformation of metabolic regulations of the host, which allow them to escape from the immune surveillance system and enhance their survival. In this study, the Vibrio splendidus agmatinase (designated as Vsa ...
DOI:: 10.1016/j.aquaculture.2018.03.055
: https://dx.doi.org/10.1016/j.aquaculture.2018.03.055

69. A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-2) serves as a multipotent pattern recognition receptor

Author:: Liu, Yu; Zhang, Peng; Wang, Weilin; Dong, Miren; Wang, Min; Gong, Changhao; Jia, Zhihao; Liu, Zhaoqun; Zhang, Anguo; Wang, Lingling; Song, Linsheng
Source:: Developmental and comparative immunology 2018 v.84 pp. 315-326
ISSN:: 0145-305X
Subject:: Staphylococcus aureus; lipopolysaccharides; Komagataella pastoris; phylogeny; genes; Gram-positive bacteria; polypeptides; Vibrio splendidus; Micrococcus luteus; open reading frames; Yarrowia lipolytica; Vibrio anguillarum; gene expression; amino acids; Crassostrea gigas; hepatopancreas; binding capacity; Drosophila melanogaster; tissues; Escherichia coli; Gram-negative bacteria; messenger RNA; Aeromonas hydrophila; muscles; mannose; oysters; protein domains; innate immunity; lectins; peptidoglycans; gills; hemocytes; fungi
Abstract:: ... DM9 is a novel protein domain with unknown function originally discovered in Drosophila melanogaster. Recently, a protein harboring DM9 repeats was identified as mannose-specific lectin (CgCGL1, renamed as CgDM9CP-1) from the Pacific oyster Crassostrea gigas. In the present study, another DM9 containing protein was identified from oyster C. gigas (designated as CgDM9CP-2). The open reading frame o ...
DOI:: 10.1016/j.dci.2018.03.003
: https://dx.doi.org/10.1016/j.dci.2018.03.003

70. Rapid baso-apical translocation of Mycobacterium avium ssp. paratuberculosis in mammary epithelial cells in the presence of Escherichia coli

Author:: Schwarz, D.G.G.; Shoyama, F.M.; Oliveira, L.L.; Sreevatsan, S.; Moreira, M.A.S.
Source:: Journal of dairy science 2018 v.101 no.7 pp. 6287-6295
ISSN:: 0022-0302
Subject:: Escherichia coli; Mycobacterium avium subsp. paratuberculosis; adhesion; animal tissues; bacteria; bovine mastitis; calves; cell lines; epithelial cells; gene expression; inflammation; interleukin-10; intestines; mammary glands; messenger RNA; milk; mitogen-activated protein kinase; mixed infection; models; paratuberculosis; pathogens; suckling
Abstract:: ... Infection of mammary gland cells with bacterial pathogens begins with adhesion, invasion, and persistence within the cells or systemic distribution. Some bacteria, such as Escherichia coli, are known to causes bovine mastitis, resulting in acute proinflammatory responses in the mammary tissue. Mycobacterium avium ssp. paratuberculosis (MAP), the etiological agent of paratuberculosis, is able to sp ...
DOI:: 10.3168/jds.2017-13945
: https://dx.doi.org/10.3168/jds.2017-13945

71. Microcystin induction small Maf protein involve in transcriptional regulation of GST from freshwater mussel Cristaria plicata

Author:: Wang, Xiaobo; Wu, Jielian; Jian, Shaoqing; Yang, Gang; Hu, Baoqing; Wen, Chungen
Source:: Gene 2018 v.660 pp. 51-61
ISSN:: 0378-1119
Subject:: Escherichia coli; amino acids; complementary DNA; freshwater mussels; gene expression; hemocytes; hepatopancreas; homeostasis; messenger RNA; microcystins; morphogenesis; open reading frames; plasmids; quantitative polymerase chain reaction; rapid amplification of cDNA ends; recombinant proteins; sequence homology; tissues; transcription (genetics); transcription factors
Abstract:: ... The small Mafs, MafF, MafG and MafK play critical roles in morphogenesis and homeostasis through associating with Cap “n” Collar family of transcription factors. In this study, we tried to identify a small Maf protein in the freshwater mussel Cristaria plicata. The MafK cDNA of C. plicata, designated as CpMafK, was cloned from the hemocytes using degenerate primers by the rapid amplification of cD ...
DOI:: 10.1016/j.gene.2018.03.041
: http://dx.doi.org/10.1016/j.gene.2018.03.041

72. A novel fucolectin from Apostichopus japonicus with broad PAMP recognition pattern

Author:: Wang, Ying; Xue, Zhuang; Yi, Qilin; Wang, Hui; Wang, Lingling; Lu, Guangxia; Liu, Yu; Qu, Chen; Li, Yannan; Song, Linsheng
Source:: Fish & shellfish immunology 2018 v.77 pp. 402-409
ISSN:: 1050-4648
Subject:: Apostichopus japonicus; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Micrococcus luteus; Vibrio splendidus; agglutination; amino acids; coelomocytes; cytoplasm; fluorescent antibody technique; fucose; fungi; gene expression; gene expression regulation; gonads; innate immunity; integument; lectins; lipopolysaccharides; messenger RNA; molecular weight; muscles; open reading frames; peptidoglycans; polypeptides; quantitative polymerase chain reaction; respiratory system; reverse transcriptase polymerase chain reaction; signal peptide
Abstract:: ... F-type lectin (also known as fucolectin) is a newly identified family of fucose binding lectins with the sequence characters of a fucose binding motif and a unique lectin fold (the “F-type” fold). In the present study, a fucolectin was identified from sea cucumber Apostichopus japonicus (designated AjFL-1). The open reading frame (ORF) of AjFL-1 was of 546 bp, encoding a polypeptide of 181 amino a ...
DOI:: 10.1016/j.fsi.2018.04.013
: https://dx.doi.org/10.1016/j.fsi.2018.04.013

73. Effects of chestnut tannins on intestinal morphology, barrier function, pro‐inflammatory cytokine expression, microflora and antioxidant capacity in heat‐stressed broilers

Author:: Liu, H. W.; Li, K.; Zhao, J. S.; Deng, W.
Source:: Journal of animal physiology and animal nutrition 2018 v.102 no.3 pp. 717-726
ISSN:: 0931-2439
Subject:: Castanea; Clostridium; Escherichia coli; antioxidant activity; average daily gain; blood serum; cecum; diamine oxidase; diet; enzyme activity; gene expression; growth performance; heat; heat stress; interleukin-6; jejunum; lactic acid; males; malondialdehyde; messenger RNA; microorganisms; superoxide dismutase; tannins; transcription factor NF-kappa B; villi
Abstract:: ... A study was conducted to evaluate the effects of chestnut tannins (CT) on intestinal morphology, barrier function, pro‐inflammatory cytokine expression, microflora and antioxidant capacity in heat‐stressed broilers. Four hundred 28‐day‐old male Ross 308 broilers were randomly assigned into four groups, with 10 replicates per group and 10 broilers per replicate. The broilers in the normal (NOR) gro ...
DOI:: 10.1111/jpn.12839
: http://dx.doi.org/10.1111/jpn.12839

74. Equisetin as potential quorum sensing inhibitor of Pseudomonas aeruginosa

Author:: Zhang, Mengmeng; Wang, Mengjia; Zhu, Xiaocui; Yu, Wengong; Gong, Qianhong
Source:: Biotechnology letters 2018 v.40 no.5 pp. 865-870
ISSN:: 0141-5492
Subject:: Escherichia coli; Fusarium; Pseudomonas aeruginosa; active ingredients; bacterial motility; biofilm; biosensors; gene expression; genes; high performance liquid chromatography; infectious diseases; marine fungi; messenger RNA; quantitative polymerase chain reaction; quorum sensing; reverse transcriptase polymerase chain reaction; secondary metabolites; therapeutics; virulence
Abstract:: ... OBJECTIVE: To screen for the quorum-sensing (QS) inhibitors from marine-derived fungi and evaluate their anti-QS properties in Pseudomonas aeruginosa. RESULTS: QS inhibitory activity was found in secondary metabolites of a marine fungus Fusarium sp. Z10 using P. aeruginosa QSIS-lasI biosensor. The major active compound of this fungus was isolated by HPLC and identified as equisetin. Subinhibitory ...
DOI:: 10.1007/s10529-018-2527-2
: https://dx.doi.org/10.1007/s10529-018-2527-2

75. Cloning, Expression Analysis and Enzyme Activity Assays of the α-Carbonic Anhydrase Gene from Chlamydomonas sp. ICE-L

Author:: Qu, Changfeng; He, Yingying; Zheng, Zhou; An, Meiling; Li, Lulu; Wang, Xixi; He, Xiaodong; Wang, Yibin; Liu, Fangming; Miao, Jinlai
Source:: Molecular biotechnology 2018 v.60 no.1 pp. 21-30
ISSN:: 1073-6085
Subject:: Chlamydomonas; Escherichia coli; bacteria; bioinformatics; carbon dioxide; enzyme activity; esterases; gene expression; genes; messenger RNA; molecular cloning; molecular weight; open reading frames; polyacrylamide gel electrophoresis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; salinity; salt stress; temperature; ultraviolet radiation; zinc
Abstract:: ... The α-carbonic anhydrase (α-CA) is a zinc ion-containing enzyme that catalyzes the hydration of carbon dioxide. In this paper, a full-length α-CA gene was cloned from Chlamydomonas sp. ICE-L using RT-PCR and RACE-PCR for bioinformatic analysis. The α-CA open reading frame obtained by PCR was cloned into a vector and transformed into Escherichia coli to generate α-CA-producing bacteria. The α-CA wa ...
DOI:: 10.1007/s12033-017-0040-9
: https://dx.doi.org/10.1007/s12033-017-0040-9

76. Identification and function of cAMP response element binding protein in Oak silkworm Antheraea pernyi

Author:: Gao, Jin; Sun, Yu; Sun, Yuxuan; Chen, Chen; Kausar, Saima; Tian, Jiwu; Zhu, Baojian; Liu, Chaoliang
Source:: Journal of invertebrate pathology 2018 v.151 pp. 14-20
ISSN:: 0022-2011
Subject:: Antheraea pernyi; Baculoviridae; Beauveria bassiana; Escherichia coli; Micrococcus luteus; RNA interference; adenosine monophosphate; binding proteins; cAMP-dependent protein kinase; cecropins; cyclic AMP; gene expression; genes; innate immunity; integument; leucine zipper; messenger RNA; microorganisms; midgut; phosphorylation; polyclonal antibodies; rabbits; silkworms
Abstract:: ... Cyclic AMP response element binding (CREB) proteins participate in the regulation of many biological processes. However, little is known about their role in immune regulation in the Oak silkworm (Antheraea pernyi). In this study, a CREB gene was identified in A. pernyi and its role in immune regulation was investigated. ApCREB shares conserved signature motifs with other CREB proteins, and include ...
DOI:: 10.1016/j.jip.2017.10.006
: https://dx.doi.org/10.1016/j.jip.2017.10.006

77. In silico identification, characterization and expression analysis of attacin gene family in response to bacterial and fungal pathogens in Tenebrio molitor

Author:: Jo, Yong Hun; Park, Soyi; Park, Ki Beom; Noh, Mi Young; Cho, Jun Ho; Ko, Hye Jin; Kim, Chang Eun; Patnaik, Bharat Bhusan; Kim, Jin; Won, Ran; Bang, In Seok; Lee, Yong Seok; Han, Yeon Soo
Source:: Entomological research 2018 v.48 no.1 pp. 45-54
ISSN:: 1738-2297
Subject:: Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Malpighian tubules; Tenebrio molitor; Tribolium castaneum; adults; amino acids; antimicrobial peptides; antimicrobial properties; databases; digestive system; fat body; fungi; gene expression; genes; hemocytes; larvae; messenger RNA; metamorphosis; pathogens; pupae; testes
Abstract:: ... Antimicrobial peptides are effector molecules induced after microbial challenges. These form important components of innate host defense against the pathogens by exhibiting wide‐spectrum antimicrobial activities. In this study, we identified three attacin‐like genes from Tenebrio molitor RNASeq database using Tribolium castaneum attacin gene family as query. The T. molitor attacin gene family was ...
DOI:: 10.1111/1748-5967.12287
: http://dx.doi.org/10.1111/1748-5967.12287

78. A GTP-dependent Phosphoenolpyruvate Carboxykinase from Crassostrea gigas Involved in Immune Recognition

Author:: Lv, Zhao; Qiu, Limei; Wang, Weilin; Liu, Zhaoqun; Xue, Zhuang; Yu, Zichao; Song, Xiaorui; Chen, Hao; Wang, Lingling; Song, Linsheng
Source:: Developmental and comparative immunology 2017 v.77 pp. 318-329
ISSN:: 0145-305X
Subject:: Crassostrea gigas; Escherichia coli; Micrococcus luteus; Staphylococcus aureus; Vibrio splendidus; active sites; bacteria; beta-glucans; binding sites; biochemical pathways; gene expression; gluconeogenesis; guanosine triphosphate; hemocytes; hepatopancreas; immune response; immunohistochemistry; lipopolysaccharides; lipoteichoic acids; messenger RNA; muscles; oysters; peptidoglycans; proteins; tissues
Abstract:: ... Phosphoenolpyruvate carboxykinase (PEPCK) is well known as a key enzyme involved in the metabolic pathway of gluconeogenesis in organisms, but the information about its involvement in immune response is still very limited. In the present study, a novel PEPCK homolog named CgPEPCK was identified from oyster Crassostrea gigas. The deduced amino acid sequence of CgPEPCK shared 52%–74% similarities wi ...
DOI:: 10.1016/j.dci.2017.09.001
: https://dx.doi.org/10.1016/j.dci.2017.09.001

79. A role of cathepsin L gene in innate immune response of crayfish (Procambarus clarkii)

Author:: Dai, Li-Shang; Chu, Sheng-Hui; Yu, Xiao-Min; Li, Yan-Yan
Source:: Fish & shellfish immunology 2017 v.71 pp. 246-254
ISSN:: 1050-4648
Subject:: Escherichia coli; Procambarus clarkii; RNA interference; amino acids; cathepsin L; complementary DNA; crayfish; gene expression; genes; hepatopancreas; immune response; innate immunity; isoelectric point; lipopolysaccharides; messenger RNA; molecular weight; pathogens; polyclonal antibodies; polyinosinic-polycytidylic acid; quantitative polymerase chain reaction; rabbits; recombinant proteins; signal peptide; tissues
Abstract:: ... Cathepsin L is one of the crucial enzyme superfamilies and involved in the immune responses. In the present study, cathepsin L gene from the red crayfish Procambarus clarkii, named PcCTSL, was cloned and characterized. The cDNA fragment of PcCTSL was 1026 bp in length, which encoded a putative protein of 341 amino acid residues with a molecular weight of 37.884 kDa. The theoretical isoelectric poi ...
DOI:: 10.1016/j.fsi.2017.10.021
: https://dx.doi.org/10.1016/j.fsi.2017.10.021

80. Benzoic acid beneficially affects growth performance of weaned pigs which was associated with changes in gut bacterial populations, morphology indices and growth factor gene expression

Author:: Chen, J. L.; Zheng, P.; Zhang, C.; Yu, B.; He, J.; Yu, J.; Luo, J. Q.; Mao, X. B.; Huang, Z. Q.; Chen, D. W.
Source:: Journal of animal physiology and animal nutrition 2017 v.101 no.6 pp. 1137-1146
ISSN:: 0931-2439
Subject:: Escherichia coli; Lactobacillus; benzoic acid; body weight; cecum; diamine oxidase; diet; feed conversion; gene expression; genes; growth performance; ileum; insulin-like growth factor I; insulin-like growth factor I receptor; jejunum; messenger RNA; occludins; pH; piglets; tight junctions; villi
Abstract:: ... This study was conducted to investigate the effects of benzoic acid (BA) on growth performance, intestinal development and intestinal barrier function in weaned pigs. Ninety weaned pigs were randomly assigned to one of three treatments: a basal diet (CON), the basal diet supplemented with 2000 mg/kg benzoic acid (BA1) and 5000 mg/kg benzoic acid (BA2). At the end of days 14 and 42, six pigs per tr ...
DOI:: 10.1111/jpn.12627
: http://dx.doi.org/10.1111/jpn.12627

81. Protective effect of cinnamic acid in endotoxin‐poisoned mice

Author:: Xu, Feng; Wang, Feng; Wen, Taoqun; Sang, Wentao; He, Xinyu; Li, Ling; Zeng, Nan
Source:: Phytotherapy research 2017 v.31 no.12 pp. 1946-1953
ISSN:: 0951-418X
Subject:: Escherichia coli; blood serum; caspase-1; cinnamic acid; endotoxins; gene expression; histology; histopathology; inflammasomes; interleukin-18; interleukin-1beta; lipopolysaccharides; lungs; messenger RNA; mice; neutrophils; poisoning; protective effect; sepsis (infection); spleen; thymus gland; tissues; tumor necrosis factor-alpha
Abstract:: ... In this work, we aimed to evaluate the protective effect of cinnamic acid (CD) on lipopolysaccharide (LPS; Escherichia coli 055:B5)‐induced endotoxin‐poisoned mice and clarify the underlying mechanisms. The mice were administrated CD 5 d before 15 mg/kg LPS challenge. 12 hr later, thymus was separated for determination of thymus indexes. Lung and spleen tissues were collected for histologic examin ...
DOI:: 10.1002/ptr.5944
: http://dx.doi.org/10.1002/ptr.5944

82. Bacillus subtilis and yeast cell wall improve the intestinal health of broilers challenged by Clostridium perfringens

Author:: Li, Z.; Wang, W.; Lv, Z.; Liu, D.; Guo, Y.
Source:: British poultry science 2017 v.58 no.6 pp. 635-643
ISSN:: 1466-1799
Subject:: Bacillus subtilis; Bifidobacterium; Clostridium perfringens; Escherichia coli; bacitracin; blood serum; cecum; cell walls; cytokines; endotoxins; feed additives; gene expression; ileum; males; messenger RNA; pathogens; yeasts; zinc
Abstract:: ... 1. The objective was to investigate the effects of Bacillus subtilis, yeast cell wall (YCW) and their combination on intestinal health of broilers challenged by Clostridium perfringens over a 21-d period. 2. Using a 5 × 2 factorial arrangement of treatments, 800 1-d-old male Cobb 500 broilers were used to study the effects of feed additives (without additive or with zinc bacitracin, B. subtilis, Y ...
DOI:: 10.1080/00071668.2017.1370697
: http://dx.doi.org/10.1080/00071668.2017.1370697

83. Colonization by non-pathogenic bacteria alters mRNA expression of cytochromes P450 in originally germ-free mice

Author:: Jourová, L.; Anzenbacher, P.; Lišková, B.; Matušková, Z.; Hermanová, P.; Hudcovic, T.; Kozáková, H.; Hrnčířová, L.; Anzenbacherová, E.
Source:: Folia microbiologica 2017 v.62 no.6 pp. 463-469
ISSN:: 0015-5632
Subject:: Escherichia coli; Lactobacillus plantarum; bacteria; biotransformation; cytochrome P-450; drugs; enzymes; gene expression; intestinal microorganisms; liver; messenger RNA; metabolism; mice; microbial colonization; probiotics; specific pathogen-free animals; xenobiotics
Abstract:: ... Gut microbiota provides a wide range of beneficial function for the host and has an immense effect on the host’s health state. It has also been shown that gut microbiome is often involved in the biotransformation of xenobiotics; however, the molecular mechanisms of the interaction between the gut bacteria and the metabolism of drugs by the host are still unclear. To investigate the effect of micro ...
DOI:: 10.1007/s12223-017-0517-8
: http://dx.doi.org/10.1007/s12223-017-0517-8

84. Functional activities of interferon gamma in large yellow croaker Larimichthys crocea

Author:: Yang, Sisi; Li, Qiuhua; Mu, Yinnan; Ao, Jingqun; Chen, Xinhua
Source:: Fish & shellfish immunology 2017 v.70 pp. 545-552
ISSN:: 1050-4648
Subject:: CD4-positive T-lymphocytes; Escherichia coli; Larimichthys crocea; bacterial vaccines; bioactive properties; gene expression; genes; granulocytes; heart; immune response; inducible nitric oxide synthase; inflammation; interferon-gamma; interleukin-1beta; interleukin-2; kidneys; lipopolysaccharides; macrophages; mammals; messenger RNA; nitric oxide; polyinosinic-polycytidylic acid; respiratory burst; signal transduction; spleen; tissues; transcription factors; tumor necrosis factor-alpha
Abstract:: ... Interferon gamma (IFN-γ) is a T helper cell type 1 (Th1) cytokine that plays important roles in almost all phases of immune and inflammatory responses. Although IFN-γ gene in large yellow croaker Larimichthys crocea has been reported, little is known about its bioactivity. In this study, large yellow croaker IFN-γ (LycIFN-γ) gene was found to be constitutively expressed in all tissues tested, with ...
DOI:: 10.1016/j.fsi.2017.09.051
: https://dx.doi.org/10.1016/j.fsi.2017.09.051

85. Serpin-14 negatively regulates prophenoloxidase activation and expression of antimicrobial peptides in Chinese oak silkworm Antheraea pernyi

Author:: Kausar, Saima; Abbas, Muhammad Nadeem; Qian, Cen; Zhu, Baojian; Sun, Yu; Sun, Yuxuan; Wang, Lei; Wei, Guoqing; Maqsood, Iram; Liu, Chao-Liang
Source:: Developmental and comparative immunology 2017 v.76 pp. 45-55
ISSN:: 0145-305X
Subject:: Antheraea pernyi; Baculoviridae; Beauveria bassiana; Escherichia coli; Micrococcus luteus; amino acids; antimicrobial peptides; complementary DNA; fat body; gene expression; genes; hemocytes; immune response; innate immunity; instars; invertebrates; larvae; melanization; messenger RNA; monophenol monooxygenase; open reading frames; polyclonal antibodies; prophenoloxidase; proteins; pupae; quantitative polymerase chain reaction; rabbits; silkworms
Abstract:: ... Genes encoding proteins of serpins superfamily are widely distributed in invertebrates. In insects, serpins play important roles in regulating immune responses and other physiological processes. Here, we report the cloning and characterization of cDNA of Apserpin-14 from Chinese oak silkworm (Antheraea pernyi). The Apserpin-14 gene contains 1206 bp open reading frame, encoding a predicted 401 amin ...
DOI:: 10.1016/j.dci.2017.05.017
: https://dx.doi.org/10.1016/j.dci.2017.05.017

86. DNA methylation differences of the BPI promoter among pig breeds and the regulation of gene expression

Author:: Wang, HaiFei; Wu, Jiayun; Wu, Sen; Wu, ShengLong; Bao, WenBin
Source:: RSC advances 2017 v.7 no.76 pp. 48025-48030
ISSN:: 2046-2069
Subject:: DNA methylation; Escherichia coli; Gram-negative bacteria; Meishan; antibacterial properties; disease susceptibility; gene expression; gene expression regulation; immunity; intestines; luciferase; messenger RNA; swine; transcription (genetics)
Abstract:: ... The bactericidal/permeability-increasing protein (BPI) exerts broad-spectrum bactericidal effects against Gram-negative bacteria and contributes to natural defenses. The expression patterns of BPI among different pig breeds and the underlying regulatory mechanisms remain elusive. In this study, we presented that the pig breeds of Yorkshire, Sutai and Meishan showed distinct BPI expression and prom ...
DOI:: 10.1039/c7ra05549h
: http://dx.doi.org/10.1039/c7ra05549h

87. Stem-Loop Structures within mRNA Coding Sequences Activate Translation Initiation and Mediate Control by Small Regulatory RNAs

Author:: Jagodnik, Jonathan; Chiaruttini, Claude; Guillier, Maude
Source:: Molecular cell 2017 v.68 no.1 pp. 158-170.e3
ISSN:: 1097-2765
Subject:: Escherichia coli; bacteria; gene expression; iron; messenger RNA; outer membrane proteins; regulatory sequences; ribosomes
Abstract:: ... Initiation is the rate-limiting step of translation, and in bacteria, mRNA secondary structure has been extensively reported as limiting the efficiency of translation by occluding the ribosome-binding site. In striking contrast with this inhibitory effect, we report here that stem-loop structures located within coding sequences instead activate translation initiation of the Escherichia coli fepA a ...
DOI:: 10.1016/j.molcel.2017.08.015
: https://dx.doi.org/10.1016/j.molcel.2017.08.015

88. Distribution of recombinant VP28 protein in tissues and its immunomodulatory effect against white spot syndrome virus in whiteleg shrimp, Litopenaeus vannamei (Boone, 1931)

Author:: Taju, G.; Madan, N.; Abdul Majeed, S.; Rajkumar, T.; Farook, M. A.; Vimal, S.; Nazeer Basha, A.; Sahul Hameed, A. S.
Source:: Aquaculture international 2017 v.25 no.5 pp. 1761-1776
ISSN:: 0967-6120
Subject:: Escherichia coli; Litopenaeus vannamei; White spot syndrome virus; antiserum; crustin; enzyme activity; enzyme-linked immunosorbent assay; gene expression; genes; heart; hemocyanin; hemocytes; hepatopancreas; immunomodulators; lectins; lipopolysaccharides; lysozyme; messenger RNA; monophenol monooxygenase; muscles; quantitative polymerase chain reaction; shrimp; sodium chloride; superoxide anion; superoxide dismutase; tissue distribution; tissues; tumor necrosis factors
Abstract:: ... The gene encoding VP28 protein of WSSV was cloned into pRSET B vector, and recombinant VP28 (r-VP28) protein was produced in E. coli GJ1158 by induction with NaCl. The r-VP28 protein was purified. The purified r-VP28 protein was injected intramuscularly in Whiteleg shrimp, Litopenaeus vannamei, to study its tissue distribution at different time intervals by ELISA using the antiserum raised against ...
DOI:: 10.1007/s10499-017-0155-9
: http://dx.doi.org/10.1007/s10499-017-0155-9

89. TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor

Author:: Kim, Soo Gon; Jo, Yong Hun; Seong, Jeong Hwan; Park, Ki Beom; Noh, Mi Young; Cho, Jun Ho; Ko, Hye Jin; Kim, Chang Eun; Tindwa, Hamisi; Patnaik, Bharat Bhusan; Bang, In Seok; Lee, Yong Seok; Han, Yeon Soo
Source:: Insect biochemistry and molecular biology 2017 v.89 pp. 31-42
ISSN:: 0965-1748
Subject:: Candida albicans; Drosophila; Escherichia coli; RNA interference; Staphylococcus aureus; Tenebrio molitor; adults; amino acids; bacteria; beta-glucans; complementary DNA; fungi; gene expression; hemocytes; immunoassays; insects; instars; larvae; ligands; low density lipoprotein; messenger RNA; pathogens; phagocytosis; polyclonal antibodies; receptors; shrimp; tissues; viruses
Abstract:: ... Scavenger receptors (SRs) constitute a family of membrane-bound receptors that bind to multiple ligands. The SR family of proteins is involved in removing cellular debris, oxidized low-density lipoproteins, and pathogens. Specifically, class C scavenger receptors (SR-C) have also been reported to be involved in phagocytosis of gram-positive and -negative bacteria in Drosophila and viruses in shrim ...
DOI:: 10.1016/j.ibmb.2017.08.007
: https://dx.doi.org/10.1016/j.ibmb.2017.08.007

90. Effects of dietary standardized ileal digestible tryptophan:lysine ratio on performance, plasma urea nitrogen, ileal histomorphology and immune responses in weaned pigs challenged with Escherichia coli K88

Author:: Jayaraman, B.; Regassa, A.; Htoo, J.K.; Nyachoti, C.M.
Source:: Livestock science 2017 v.203 pp. 114-119
ISSN:: 1871-1413
Subject:: Duroc; Escherichia coli; body weight; experimental diets; gene expression; ileum; immune response; interferon-gamma; interleukin-10; landraces; messenger RNA; piglets; quantitative polymerase chain reaction; regression analysis; reverse transcriptase polymerase chain reaction; starter diets; tumor necrosis factor-alpha; urea nitrogen
Abstract:: ... A study was conducted to determine the optimal standardized ileal digestible (SID) tryptophan:lysine (Trp:Lys) ratio in piglets challenged with Escherichia coli K88 (E.coli K88) and fed antibiotic-free diets. Thirty individually housed mixed-sex pigs (Duroc × [Yorkshire × Landrace]) with an initial body weight (BW) of 6.4 ± 0.18kg and weaned at 21 ± 1day (d) were randomly assigned to 5 dietary tre ...
DOI:: 10.1016/j.livsci.2017.07.014
: https://dx.doi.org/10.1016/j.livsci.2017.07.014

91. Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli

Author:: Li, Tao; Ding, Yatong; Zhang, Jun; Jiao, Guobao; Sun, Lipeng; Liu, Zhongmin; Qiu, Liyou
Source:: Electronic Journal of Biotechnology 2017 v.29 pp. 63-67
ISSN:: 0717-3458
Subject:: DNA fragmentation; Escherichia coli; Klebsiella; biotechnology; culture media; enzyme activity; fermentation; gene expression; genes; messenger RNA; plasmids; pullulanase; transcription (genetics)
Abstract:: ... Pullulanase production in both wild-type strains and recombinantly engineered strains remains low. The Shine-Dalgarno (SD) sequence and stem-loop structure in the 5′ or 3′ untranslated region (UTR) are well-known determinants of mRNA stability. This study investigated the effect of mRNA stability on pullulanase heterologous expression.We constructed four DNA fragments, pulA, SD-pulA, pulA-3t, and ...
DOI:: 10.1016/j.ejbt.2017.07.001
: http://dx.doi.org/10.1016/j.ejbt.2017.07.001

92. Anti-inflammatory effect of Man-Pen-Fang, a Chinese herbal compound, on chronic pelvic inflammation in rats

Author:: Zhang, Li-Jun; Zhu, Jian-Yong; Sun, Meng-Yao; Song, Ya-Nan; Rahman, Khalid; Peng, Cheng; Zhang, Miao; Ye, Yu-Mei; Zhang, Hong
Source:: Journal of ethnopharmacology 2017 v.208 pp. 57-65
ISSN:: 0378-8741
Subject:: Escherichia coli; Euonymus alatus; Gleditsia sinensis; Liliaceae; Oriental traditional medicine; Smilax china; Staphylococcus aureus; Streptococcus; Thlaspi arvense; Vaccaria hispanica subsp. hispanica; adults; animal models; anti-inflammatory activity; apoptosis; blood serum; enzyme-linked immunosorbent assay; gene expression; immunohistochemistry; inflammation; interleukin-10; interleukin-2; interleukin-6; messenger RNA; rats; reverse transcriptase polymerase chain reaction; tissues; transcription factor NF-kappa B; transforming growth factor beta 1; tumor necrosis factor-alpha; uterus
Abstract:: ... Traditional Chinese Medicine (TCM) has become the focus of research for the treatment of chronic pelvic inflammatory disease (CPID) based on unique medical theory system. Man-Pen-Fang (MPF), a Chinese herbal compound, which is composed of Thlaspi arvense L. (Cruciferae), Gleditsia sinensis Lam. (Leguminosae), Smilax china L. (Liliaceae), Euonymus alatus (Thunb.) Sieb. (Celastraceae) and Vaccaria s ...
DOI:: 10.1016/j.jep.2017.06.034
: https://dx.doi.org/10.1016/j.jep.2017.06.034

93. Overexpression of Mn-superoxide dismutase in Oxya chinensis mediates increased malathion tolerance

Author:: Wu, Haihua; Zhang, Yiwei; Shi, Xuekai; Zhang, Jianzhen; Ma, Enbo
Source:: Chemosphere 2017 v.181 pp. 352-359
ISSN:: 0045-6535
Subject:: Escherichia coli; Oxya; RNA interference; dose response; gene expression; genes; malathion; messenger RNA; oxidative stress; plasmids; proteins; reactive oxygen species; superoxide dismutase; transcription (genetics)
Abstract:: ... Superoxide dismutase (SOD) is the first line of defense against oxidative damage. Malathion is an organophosphate insecticide and can induce the production of reactive oxygen species (ROS) and cause the intracellular oxidative stress. The present study was undertaken to examine the effects of malathion on SODs activity and their transcriptional levels in Oxya chinensis (Thunberg) (Orthoptera: Acri ...
DOI:: 10.1016/j.chemosphere.2017.04.087
: http://dx.doi.org/10.1016/j.chemosphere.2017.04.087

94. Trojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas

Author:: Payton, Laura; Perrigault, Mickael; Bourdineaud, Jean-Paul; Marcel, Anjara; Massabuau, Jean-Charles; Tran, Damien
Source:: Marine biotechnology 2017 v.19 no.4 pp. 361-371
ISSN:: 1436-2228
Subject:: Alexandrium minutum; Crassostrea gigas; Escherichia coli; Nematoda; RNA interference; bioaccumulation; circadian rhythm; diploidy; double-stranded RNA; food availability; gene expression; genes; insects; messenger RNA; oysters; poisonous algae; toxins; triploidy
Abstract:: ... RNA interference is a powerful method to inhibit specific gene expression. Recently, silencing target genes by feeding has been successfully carried out in nematodes, insects, and small aquatic organisms. A non-invasive feeding-based RNA interference is reported here for the first time in a mollusk bivalve, the pacific oyster Crassostrea gigas. In this Trojan horse strategy, the unicellular alga H ...
DOI:: 10.1007/s10126-017-9761-9
: http://dx.doi.org/10.1007/s10126-017-9761-9

95. Molecular and biochemical characterization of squalene synthase from Siraitia grosvenorii

Author:: Su, Heling; Liu, Yongming; Xiao, Yalun; Tan, Yanlian; Gu, Yunyan; Liang, Bin; Huang, Hongli; Wu, Yaosheng
Source:: Biotechnology letters 2017 v.39 no.7 pp. 1009-1018
ISSN:: 0141-5492
Subject:: Escherichia coli; Siraitia grosvenorii; amino acids; catalytic activity; chemical structure; gene expression; genes; leaves; messenger RNA; molecular cloning; pH; phosphorylation; squalene
Abstract:: ... OBJECTIVES: To clone and characterize the squalene synthase from Siraitia grosvenorii (SgSQS). RESULTS: The gene encoding SgSQS was cloned. SgSQS has 417 amino acid residues with an pI of 7.3. There are 32 phosphorylation sites in its sequence: S⁴⁸ as well as S¹⁹⁶ play important roles in regulation of enzyme activity. The enzyme is a monomeric protein with a cave-like active center formed by α hel ...
DOI:: 10.1007/s10529-017-2328-z
: http://dx.doi.org/10.1007/s10529-017-2328-z

96. Molecular cloning, expression and antioxidative activity of 2-cys-peroxiredoxin from freshwater mussel Cristaria plicata

Author:: Wang, Xiaobo; Hu, Baoqing; Wen, Chungen; Zhang, Ming; Jian, Shaoqing; Yang, Gang
Source:: Fish & shellfish immunology 2017 v.66 pp. 254-263
ISSN:: 1050-4648
Subject:: Aeromonas hydrophila; Escherichia coli; antioxidant activity; complementary DNA; cysteine; freshwater mussels; gene expression; gene expression regulation; gills; hemocytes; hepatopancreas; hydrogen peroxide; hydroperoxides; messenger RNA; molecular cloning; open reading frames; oxidative stress; peptidoglycans; peroxiredoxin; plasmids; quantitative polymerase chain reaction; recombinant proteins; sequence homology
Abstract:: ... Peroxiredoxins (Prxs) play an important role against various oxidative stresses by catalyzing the reduction of hydrogen peroxide (H2O2) and organic hydroperoxides to less harmful form. A 2-cys peroxiredoxin, designated as CpPrx, was cloned from hemocytes of freshwater mussel Cristaria plicata. The full length cDNA of CpPrx is 1247 bp, which includes an open reading frame (ORF) of 591bp, encoding 1 ...
DOI:: 10.1016/j.fsi.2017.05.026
: https://dx.doi.org/10.1016/j.fsi.2017.05.026

97. Aspartate attenuates intestinal injury and inhibits TLR4 and NODs/NF-κB and p38 signaling in weaned pigs after LPS challenge

Author:: Wang, Haibo; Liu, Yulan; Shi, Haifeng; Wang, Xiuying; Zhu, Huiling; Pi, Dingan; Leng, Weibo; Li, Shuang
Source:: European journal of nutrition 2017 v.56 no.4 pp. 1433-1443
ISSN:: 1436-6207
Subject:: Escherichia coli; Toll-like receptor 4; aspartic acid; blood; caspase-3; diamine oxidase; diet; enzyme activity; gene expression; genes; ileum; inflammation; jejunum; lipopolysaccharides; messenger RNA; oligomerization; phosphorylation; piglets; protein synthesis; signal transduction; transcription factor NF-kappa B; tumor necrosis factor-alpha; villi
Abstract:: ... PURPOSE: This study was conducted to investigate whether aspartate (Asp) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by modulating intestine inflammatory response. METHODS: Twenty-four weaned piglets were divided into four treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 0.5 % Asp; and (4) LPS + 1.0 % Asp. After feeding with cont ...
DOI:: 10.1007/s00394-016-1189-x
: http://dx.doi.org/10.1007/s00394-016-1189-x

98. Effects of intrauterine infusion of Escherichia coli lipopolysaccharide on uterine mRNA gene expression and peripheral polymorphonuclear leukocytes in Jersey cows diagnosed with purulent vaginal discharge

Author:: Moraes, João G.N.; Mendonça, Luís G.D.; Silva, Paula R.B.; Scanavez, Alexandre A.; Galvão, Klibs N.; Ballou, Michael A.; Worku, Milli; Chebel, Ricardo C.
Source:: Journal of dairy science 2017 v.100 no.6 pp. 4784-4796
ISSN:: 0022-0302
Subject:: Escherichia coli; Jersey; Toll-like receptor 4; adhesion; body condition; cell adhesion; chemical elements; cortisol; cows; gene expression; haptoglobins; hematologic tests; immunomodulators; inflammation; intercellular adhesion molecule-1; interleukin-10; interleukin-1beta; interleukin-6; interleukin-8; intrauterine administration; lactation number; lipopolysaccharides; messenger RNA; milk yield; necrosis; neutrophils; phagocytosis; progesterone; tumor necrosis factor-alpha; uterus; New Zealand
Abstract:: ... The objectives of the current experiment were to investigate the effects of intrauterine treatment of cows with purulent vaginal discharge (PVD) using lipopolysaccharide (LPS) from Escherichia coli on uterine mRNA expression of genes related to inflammatory responses, peripheral polymorphonuclear leukocyte (PMN) function, hematological parameters, and blood concentrations of cortisol, haptoglobin, ...
DOI:: 10.3168/jds.2016-11643
: https://dx.doi.org/10.3168/jds.2016-11643

99. Identification of a C-type lectin from Antheraea pernyi, which may associate with the immune response to pathogens

Author:: Qian, Cen; Lin, Kunzhang; Zhu, Baojian; Liu, Chaoliang; Wang, Lei; Liu, Ya; Liang, Dan; Wang, Pei; Wei, Guoqing
Source:: Journal of Asia-Pacific entomology 2017 v.20 no.2 pp. 505-512
ISSN:: 1226-8615
Subject:: Antheraea pernyi; Baculoviridae; Escherichia coli; agglutination; amino acids; antibodies; complementary DNA; fat body; gene expression; genes; hemolymph; innate immunity; lectins; messenger RNA; open reading frames; pathogens; recombinant proteins
Abstract:: ... C-Type lectins (CTLs) are carbohydrate-recognition proteins and play critical roles in innate immunity. In this study, we characterized a CTL from Antheraea pernyi, named ApCTL, which has two carbohydrate-recognition domains (CRDs). A fragment of 1017bp cDNA of ApCTL was isolated, which constituted 927bp open reading frame (ORF), and it encoded putative protein of 308 amino acids. The recombinant ...
DOI:: 10.1016/j.aspen.2017.03.010
: https://dx.doi.org/10.1016/j.aspen.2017.03.010

100. Characterization of galectin-1 from Chinese giant salamanders Andrias davidianus and its involvements during immune response

Author:: Yang, Hui; Lan, Qingjing; Liu, Ranran; Cui, Dan; Liu, Haixia; Xiong, Dongmei; Li, Fenggang; Liu, Xiaolin; Wang, Lixin
Source:: Developmental and comparative immunology 2017 v.70 pp. 59-68
ISSN:: 0145-305X
Subject:: Aeromonas hydrophila; Andrias; Escherichia coli; Gram-positive bacteria; Iridovirus; amino acids; apoptosis; cell adhesion; complementary DNA; gene expression; immune response; immune system; inflammation; innate immunity; kidneys; messenger RNA; open reading frames; salamanders and newts
Abstract:: ... Galectins are considered as a multifunctional protein which play essential roles in cell adhesion and apoptosis, inflammation, tumor progression and immune response. In spite of extensive studies of galectin importance in immune system among different animals, few studies have been devoted to their functions in amphibian. In the present study, we characterized one proto type of galectin (named AdG ...
DOI:: 10.1016/j.dci.2017.01.004
: https://dx.doi.org/10.1016/j.dci.2017.01.004

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1. Heme oxygenase 1 plays a crucial role in swamp eel response to oxidative stress induced by cadmium exposure or Aeromonas hydrophila infection

2. Effects of dietary pyrroloquinoline quinone disodium supplementation on inflammatory responses, oxidative stress, and intestinal morphology in broiler chickens challenged with lipopolysaccharide

3. Effects of lipopolysaccharide exposure in primary bovine ruminal epithelial cells

4. Potential Probiotic Lactobacillus rhamnosus (MTCC-5897) Inhibits Escherichia coli Impaired Intestinal Barrier Function by Modulating the Host Tight Junction Gene Response

5. Molecular characterization and biological function of a tandem-repeat galectin-9 in Qihe crucian carp Carassius auratus

6. Short communication: Effects of moringa extract on adhesion and invasion of Escherichia coli O55 in bovine mammary epithelial cells

7. Effects of spermine on ileal physical barrier, antioxidant capacity, metabolic profile and large intestinal bacteria in piglets

8. Alterations of peripheral gene expression in response to lipopolysaccharide-induced synovitis as a model for inflammation in horses

9. Molecular cloning and characterization of a cathepsin L-like cysteine protease of Angiostrongylus cantonensis

10. Detection of β-defensins and Other Antibacterial Peptides in P-glycoprotein Expressing Human Gastrointestinal Cell Lines and Their Secretions

11. A tandem-repeat galectin-1 from Apostichopus japonicus with broad PAMP recognition pattern and antibacterial activity

12. Dietary l-arginine supplementation ameliorates inflammatory response and alters gut microbiota composition in broiler chickens infected with Salmonella enterica serovar Typhimurium

13. Progesterone inhibits inflammatory response in E.coli- or LPS-Stimulated bovine endometrial epithelial cells by NF-κB and MAPK pathways

14. Alteration of insulin signaling to control insect pest by using transformed bacteria expressing dsRNA

15. Dietary genistein supplementation improves intestinal mucosal barrier function in Escherichia coli O78-challenged broilers

16. Lso-HPE1, an Effector of ‘Candidatus Liberibacter solanacearum’, Can Repress Plant Immune Response

17. Safety Assessment of Potential Probiotic Lactobacillus fermentum MTCC-5898 in Murine Model after Repetitive Dose for 28 Days (Sub-Acute Exposure)

18. Exploring bacterial pathogen community dynamics in freshwater beach sediments: A tale of two lakes

19. Meloxicam affects the inflammatory responses of bovine mammary epithelial cells

20. Characterization of a new il-4/13 homologue in grass carp (Ctenopharyngodon idella) and its cooperation with M-CSF to promote macrophage proliferation

21. Functional analysis and gene expression profiling of extracellular cathepsin Z in red sea bream, Pagrus major

22. Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum

23. Involvement of gamma interferon inducible lysosomal thiol reductase in the innate immune responses of red swamp crayfish, Procambarus clarkii

24. PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL

25. The effect and mechanism of urban fine particulate matter (PM2.5) on horizontal transfer of plasmid-mediated antimicrobial resistance genes

26. Effects of Lactococcus lactis MG1363 producing fusion proteins of bovine lactoferricin–lactoferrampin on growth, intestinal morphology and immune function in weaned piglet

27. Separation, identification and gene expression analysis of PmAMP-1 from Pinctada fucata martensii

28. Supplementation exogenous bile acid improved growth and intestinal immune function associated with NF-κB and TOR signalling pathways in on-growing grass carp (Ctenopharyngodon idella): Enhancement the effect of protein-sparing by dietary lipid

29. Establishment and characterization of two head kidney macrophage cell lines from large yellow croaker (Larimichthys crocea)

30. Improved measurement of absolute mRNA quantity without reverse transcription

31. Effect of chrysin on changes in intestinal environment and microbiome induced by fructose-feeding in rats

32. A superoxide dismutase (MnSOD) with identification and functional characterization from the freshwater mussel Cristaria plicata

33. Putrescine enhances intestinal immune function and regulates intestinal bacteria in weaning piglets

34. Effects of encapsulated organic acids and essential oils on intestinal barrier, microbial count, and bacterial metabolites in broiler chickens

35. Identification and molecular characterization of a novel anti-lipopolysaccharide factor (ALF) from red swamp crayfish, Procambarus clarkii

36. Molecular characterization and functional analysis of peroxiredoxin3 cDNA from black tiger shrimp (Penaeus monodon)

37. Molecular cloning and characterization of SOCS2 from the mealworm beetle Tenebrio molitor

38. Escherichia coli as a Model for the Description of the Antimicrobial Mechanism of a Cationic Polymer Surface: Cellular Target and Bacterial Contrast Response

39. Alteration of the phagocytosis and antimicrobial defense of Octodonta nipae (Coleoptera: Chrysomelidae) pupae to Escherichia coli following parasitism by Tetrastichus brontispae (Hymenoptera: Eulophidae)

40. Characterization of small GTPase Rac1 and its interaction with PAK1 in crayfish Procambarus clarkii

41. Characterization of two copper/zinc superoxide dismutases (Cu/Zn-SODs) from the desert beetle Microdera punctipennis and their activities in protecting E. coli cells against cold

42. Molecular cloning of polymeric immunoglobulin receptor-like (pIgRL) in flounder (Paralichthys olivaceus) and its expression in response to immunization with inactivated Vibrio anguillarum

43. The transcription factor NemR is an electrophile-sensing regulator important for the detoxification of reactive electrophiles in Acinetobacter nosocomialis

44. Anti-inflammation effects of fucosylated chondroitin sulphate from Acaudina molpadioides by altering gut microbiota in obese mice

45. Eukaryotic Acquisition of a Bacterial Operon

46. A sigma factor RpoD negatively regulates temperature-dependent metalloprotease expression in a pathogenic Vibrio splendidus

47. Characterization and in vitro expression of arginine kinase gene in the invasive western flower thrips, Frankliniella occidentalis

48. Algae-derived β-glucan enhanced gut health and immune responses of weaned pigs experimentally infected with a pathogenic E. coli

49. Identification of porcine CTLA4 gene polymorphism and their association with piglet diarrhea and performance traits

50. CpGSTd3 is a lambda-Cyhalothrin Metabolizing Glutathione S-Transferase from Cydia pomonella (L.)

51. Multi-antigenic recombinant subunit vaccine against Lawsonia intracellularis: The etiological agent of porcine proliferative enteropathy

52. Characterization of PGRP-S1 from the oriental armyworm, Mythimna separata

53. Delineation of the functional and structural properties of the glutathione transferase family from the plant pathogen Erwinia carotovora

54. Effects of prophage regions in a plasmid carrying a carbapenemase gene on survival against antibiotic stress

55. Novel Ca2+-independent C-type lectin involved in immune defense of the razor clam Sinonovacula constricta

56. Physiologic and innate immunity responses to bacterial lipopolysaccharide administration in beef heifers supplemented with OmniGen-AF

57. Molecular characterization and functional analysis of IL-12p40 from Chinese sea bass (Lateolabrax maculatus) under biotic and abiotic stresses

58. Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes

59. Avian pathogenic Escherichia coli-induced activation of chicken macrophage HD11 cells

60. Molecular characterization, expression and immune functions of cystatin B in Japanese flounder (Paralichthys olivaceus)

61. The acute stresses role of the atypical 2-cys peroxiredoxin PmPrx5 in black tiger shrimp (Penaeus monodon) from biological immunity and environmental toxicity stress

62. Molecular Characterization of a Mitochondrial Manganese Superoxide Dismutase From Chilo suppressalis (Lepidoptera: Crambidae)

63. A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action

64. Identification of two IL-4/13 homologues in large yellow croaker (Larimichthys crocea) revealed their similar roles in inducing alternative activation of monocytes/macrophages

65. Isolation, expression, and functional analysis of developmentally regulated plasma membrane polypeptide 1 (DREPP1) in Sporobolus virginicus grown under alkali salt stress

66. Vitamin E alleviates phoxim-induced toxic effects on intestinal oxidative stress, barrier function, and morphological changes in rats

67. Molecular characteristics, expression, and antimicrobial activities of i-type lysozyme from the razor clam Sinonovacula constricta

68. Identification of agmatinase from Vibrio splendidus and its roles in modulating arginine metabolism of Apostichopus japonicus

69. A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-2) serves as a multipotent pattern recognition receptor

70. Rapid baso-apical translocation of Mycobacterium avium ssp. paratuberculosis in mammary epithelial cells in the presence of Escherichia coli

59. Avian pathogenic Escherichia coli-induced activation of chicken macrophage HD11 cells