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1. A bean cDNA expressed during a hypersensitive reaction encodes a putative calcium-binding protein

Author:
Jakobek, J.L.; Smith-Baker, J.A.; Lindgren, P.B.
Source:
Molecular plant-microbe interactions 1999 v.12 no.8 pp. 712-719
ISSN:
0894-0282
Subject:
Phaseolus vulgaris; complementary DNA; gene expression; calcium-binding proteins; defense mechanisms; Pseudomonas syringae pv. tabaci; Escherichia coli; Pseudomonas fluorescens; disease resistance; apoptosis; messenger RNA; amino acid sequences; nucleotide sequences; molecular weight; Tobacco necrosis virus; biochemical pathways; binding sites; hypersensitive response
Abstract:
... The hypersensitive reaction (HR) is an inducible plant response that is associated with disease resistance. It is characterized by rapid, localized cell death at the site of infection and is believed to inhibit the spread of invading pathogens. We have isolated a cDNA clone, designated Hra32 (for hypersensitive reaction associated), corresponding to an RNA transcript that accumulates in bean durin ...
DOI:
10.1094/MPMI.1999.12.8.712
PubMed:
10432637

2. Acetyl-CoA:benzylalcohol acetyltransferase--an enzyme involved in floral scent production in Clarkia breweri

Author:
Dudareva, N.; D'Auria, J.C.; Nam, K.H.; Raguso, R.A.; Pichersky, E.
Source:
The plant journal 1998 v.14 no.3 pp. 297-304
ISSN:
0960-7412
Subject:
volatile compounds; messenger RNA; buds; Escherichia coli; flowering; complementary DNA; corolla; amino acid sequences; gene expression; Clarkia; esters; nucleotide sequences; odors; enzyme activity; leaves; acyltransferases
Abstract:
... Volatile esters impart distinct characteristics to the floral scent of many plants, and are important in attracting insect pollinators. They are also important flavor compounds in fruits. The ester benzylacetate is a major constituent of the floral scent of Clarkia breweri, an annual plant native to California. The enzyme acetyl-CoA:benzylalcohol acetyltransferase (BEAT), which catalyzes the forma ...
DOI:
10.1046/j.1365-313X.1998.00121.x
PubMed:
9628024

3. A clone-based transcriptomics approach for the identification of genes relevant for itaconic acid production in Aspergillus

Author:
Li, An; van Luijk, Nicole; ter Beek, Marloes; Caspers, Martien; Punt, Peter; van der Werf, Mariet
Source:
Fungal genetics and biology 2011 v.48 no.6 pp. 602-611
ISSN:
1087-1845
Subject:
Aspergillus niger; Aspergillus terreus; Escherichia coli; batch fermentation; citric acid; clones; complementary DNA; gene expression; genes; messenger RNA; sequence analysis; transcriptomics; transporters; zinc finger motif
Abstract:
... Several Aspergillus species are well-known for the production of a variety of organic acids. In this study, a cloned based transcriptomics approach was used to identify genes crucial in the biosynthesis pathway for one of these acids, itaconic acid. From a number of different Aspergillus terreus controlled batch fermentations, those cultures with the largest difference in itaconic acid titer and p ...
DOI:
10.1016/j.fgb.2011.01.013
PubMed:
21324422

4. Actin-depolymerizing factor of second-generation merozoite in Eimeria tenella: clone, prokaryotic expression, and diclazuril-induced mRNA expression

Author:
Zhou, Bian-hua; Wang, Hong-wei; Xue, Fei-qun; Wang, Xiao-yang; Yang, Feng-kun; Ban, Man-man; Xin, Rui-xiang; Wang, Cong-cong
Source:
Parasitology research 2010 v.106 no.3 pp. 571-576
ISSN:
0932-0113
Subject:
Eimeria tenella; Escherichia coli; actin; complementary DNA; diclazuril; gene expression; genes; merozoites; messenger RNA; open reading frames; parasites; polyacrylamide gel electrophoresis; reverse transcriptase polymerase chain reaction
Abstract:
... Actin depolymerizing factor (ADF) is an essential actin-binding protein that plays a key role in the control of actin dynamics and actin-based motility processes in intracellular parasites. To determine the effects of diclazuril on ADF gene of second-generation merozoites (mz-ADF) mRNA expression in Eimeria tenella, mz-ADF gene was cloned by RT-PCR from extracted RNA in second-generation merozoite ...
DOI:
10.1007/s00436-009-1699-z
PubMed:
20020308

5. A C-type lectin (AiCTL-3) from bay scallop Argopecten irradians with mannose/galactose binding ability to bind various bacteria

Author:
Huang, Mengmeng; Song, Xiaoyan; Zhao, Jianmin; Mu, Changkao; Wang, Lingling; Zhang, Huan; Zhou, Zhi; Liu, Xiaolin; Song, Linsheng
Source:
Gene 2013 v.531 pp. 31-38
ISSN:
0378-1119
Subject:
Argopecten irradians; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Micrococcus luteus; Staphylococcus aureus; Vibrio anguillarum; amino acids; binding sites; calcium; carbohydrate binding; complementary DNA; expressed sequence tags; galactose; gene expression; heart; hemocytes; hepatopancreas; immune response; lectins; mannose; messenger RNA; muscles; phagocytosis; rapid amplification of cDNA ends; scallops; vertebrates
Abstract:
... C-type lectins are a family of Ca2+-dependent carbohydrate-binding proteins playing crucial roles in innate immunity of vertebrates and invertebrates. In the present study, the cDNA of a C-type lectin with one carbohydrate-recognition domain (CRD) of 127 amino acids was cloned from bay scallop Argopecten irradians (designated AiCTL-3) by rapid amplification of cDNA end (RACE) techniques based on e ...
DOI:
10.1016/j.gene.2013.08.042
PubMed:
24008017

6. A cysteine protease from maize isolated in a complex with cystatin

Author:
Yamada, T.; Ohta, H.; Shinohara, A.; Iwamatsu, A.; Shimada, H.; Tsuchiya, T.; Masuda, T.; Takamiya, K.
Source:
Plant & cell physiology 2000 v.41 no.2 pp. 185-191
ISSN:
0032-0781
Subject:
plant tissues; genetically modified organisms; Zea mays; messenger RNA; Escherichia coli; complementary DNA; amino acid sequences; gene expression; nucleotide sequences; proteinase inhibitors; molecular weight; cysteine proteinases
DOI:
10.1093/pcp/41.2.185
PubMed:
10795313

7. A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action

Author:
Yang, Dinglong; Zhang, Qianqian; Wang, Qing; Chen, Lizhu; Liu, Yongliang; Cong, Ming; Wu, Huifeng; Li, Fei; Ji, Chenglong; Zhao, Jianmin
Source:
Fish & shellfish immunology 2018 v.80 pp. 274-280
ISSN:
1050-4648
Subject:
Escherichia coli; Gram-negative bacteria; Ruditapes philippinarum; Vibrio anguillarum; Vibrio splendidus; amino acids; antibacterial properties; antimicrobial peptides; cell death; clams; fish; gene expression; gene expression regulation; hemocytes; hepatopancreas; innate immunity; isoelectric point; mechanism of action; membrane permeability; messenger RNA; molecular weight; open reading frames; phylogeny; polypeptides; proteins; shellfish; synthetic peptides; tissues
Abstract:
... Defensins are small cysteine-rich cationic proteins that are ubiquitously present in both vertebrates and invertebrates and constitute the front line of host innate immunity. In the present study, a defensin-like antimicrobial peptide (designed as RpdefB) was identified and characterized from the manila clam Ruditapes philippinarum. The open reading frame of RpdefB encoded a 70-amino acid polypept ...
DOI:
10.1016/j.fsi.2018.06.019

8. A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-2) serves as a multipotent pattern recognition receptor

Author:
Liu, Yu; Zhang, Peng; Wang, Weilin; Dong, Miren; Wang, Min; Gong, Changhao; Jia, Zhihao; Liu, Zhaoqun; Zhang, Anguo; Wang, Lingling; Song, Linsheng
Source:
Developmental and comparative immunology 2018 v.84 pp. 315-326
ISSN:
0145-305X
Subject:
Staphylococcus aureus; lipopolysaccharides; Komagataella pastoris; phylogeny; genes; Gram-positive bacteria; polypeptides; Vibrio splendidus; Micrococcus luteus; open reading frames; Yarrowia lipolytica; Vibrio anguillarum; gene expression; amino acids; Crassostrea gigas; hepatopancreas; binding capacity; Drosophila melanogaster; tissues; Escherichia coli; Gram-negative bacteria; messenger RNA; Aeromonas hydrophila; muscles; mannose; oysters; protein domains; innate immunity; lectins; peptidoglycans; gills; hemocytes; fungi
Abstract:
... DM9 is a novel protein domain with unknown function originally discovered in Drosophila melanogaster. Recently, a protein harboring DM9 repeats was identified as mannose-specific lectin (CgCGL1, renamed as CgDM9CP-1) from the Pacific oyster Crassostrea gigas. In the present study, another DM9 containing protein was identified from oyster C. gigas (designated as CgDM9CP-2). The open reading frame o ...
DOI:
10.1016/j.dci.2018.03.003

9. A double WAP domain-containing protein from Chinese mitten crab Eriocheir sinensis with antimicrobial activities against Gram-negative bacteria and yeast

Author:
Li, Fengmei; Wang, Lingling; Qiu, Limei; Zhang, Huan; Gai, Yunchao; Song, Linsheng
Source:
Developmental and comparative immunology 2012 v.36 no.1 pp. 183-190
ISSN:
0145-305X
Subject:
Candida parapsilosis; Komagataella pastoris; isoelectric point; polypeptides; tissue repair; Eriocheir sinensis; open reading frames; Vibrio anguillarum; gene expression; whey; signal peptide; molecular weight; cysteine; tissues; Escherichia coli; Gram-negative bacteria; messenger RNA; complementary DNA; polymerase chain reaction; yeasts; immune response; proteins; innate immunity; expressed sequence tags; anti-infective properties; hemocytes
Abstract:
... The whey acidic protein (WAP) domain is characterized by a ‘four-disulfide-core’ (4-DSC) motif comprising of approximately 50 amino acids with eight highly conserved cysteine residues. Previous research indicated that WAP domain-containing proteins played an important role in the innate immunity of crustaceans. In the present study, a novel double WAP domain (DWD)-containing protein gene was ident ...
DOI:
10.1016/j.dci.2011.07.003

10. A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization

Author:
Huang, Mengmeng; Wang, Lingling; Yang, Jialong; Zhang, Huan; Wang, Leilei; Song, Linsheng
Source:
Developmental and comparative immunology 2013 v.39 no.4 pp. 363-369
ISSN:
0145-305X
Subject:
Staphylococcus aureus; Komagataella pastoris; Chlamys; receptors; phagocytosis; Gram-positive bacteria; Micrococcus luteus; immunohistochemistry; polyclonal antibodies; glucans; Vibrio anguillarum; gene expression; hepatopancreas; Escherichia coli; Gram-negative bacteria; messenger RNA; mannose; kidneys; lectins; scallops; hemocytes; fungi
Abstract:
... C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (CRD), which participate in nonself-recognition and clearance of invaders. In invertebrate, some multidomain C-type lectins have been identified, but their relative functions and binding mechanism are still meager. In the present study, A C-type lectin (Cf ...
DOI:
10.1016/j.dci.2012.12.002

11. A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes encapsulation

Author:
Wang, Mengqiang; Wang, Lingling; Huang, Mengmeng; Yi, Qilin; Guo, Ying; Gai, Yunchao; Wang, Hao; Zhang, Huan; Song, Linsheng
Source:
Fish & shellfish immunology 2016 v.55 pp. 10-20
ISSN:
1050-4648
Subject:
Staphylococcus aureus; crabs; lipopolysaccharides; Komagataella pastoris; receptors; polypeptides; Micrococcus luteus; Eriocheir sinensis; open reading frames; glucans; proline; rapid amplification of cDNA ends; Vibrio anguillarum; gene expression; hepatopancreas; agglutination; tissues; pathogens; Escherichia coli; messenger RNA; complementary DNA; amino acid sequences; encapsulation; innate immunity; galactose; lectins; vertebrates; expressed sequence tags; microorganisms; peptidoglycans; agarose; Bacillus subtilis; hemocytes
Abstract:
... Galectins are a family of β-galactoside binding lectins that function as pattern recognition receptors (PRRs) in innate immune system of both vertebrates and invertebrates. The cDNA of Chinese mitten crab Eriocheir sinensis galectin (designated as EsGal) was cloned via rapid amplification of cDNA ends (RACE) technique based on expressed sequence tags (ESTs) analysis. The full-length cDNA of EsGal ...
DOI:
10.1016/j.fsi.2016.04.019

12. A galectin related protein from Oplegnathus fasciatus: Genomic, molecular, transcriptional features and biological responses against microbial pathogens

Author:
Thulasitha, William Shanthakumar; Whang, Ilson; Umasuthan, Navaneethaiyer; Kang, Hyun-Sil; Mothishri, M.S.; Lee, Seongdo; Qiang, Wan; Noh, Jae Koo; Lee, Jehee
Source:
Developmental and comparative immunology 2016 v.56 pp. 13-24
ISSN:
0145-305X
Subject:
Edwardsiella tarda; Escherichia coli; Listeria monocytogenes; Miamiensis avidus; Oplegnathus fasciatus; Streptococcus iniae; Vibrio tapetis; amino acids; bacteria; bacterial artificial chromosomes; bream; carbohydrate binding; complement; complementary DNA; exons; gene expression; genomic libraries; gills; introns; lectins; messenger RNA; open reading frames; parasites; pathogens; phagocytosis; phylogeny; receptors; sequence alignment; signal peptide; spleen; transcription (genetics)
Abstract:
... Galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. Here, we report a novel galectin related protein (GRP) identified from rock bream (Oplegnathus fasciatus), designated OfGal like B. The cDNA of O ...
DOI:
10.1016/j.dci.2015.11.011

13. A galectin with quadruple-domain from bay scallop Argopecten irradians is involved in innate immune response

Author:
Song, Xiaoyan; Zhang, Huan; Wang, Lingling; Zhao, Jianmin; Mu, Changkao; Song, Linsheng; Qiu, Limei; Liu, Xiaolin
Source:
Developmental and comparative immunology 2011 v.35 no.5 pp. 592-602
ISSN:
0145-305X
Subject:
Komagataella pastoris; polypeptides; Micrococcus luteus; open reading frames; cell-mediated immunity; rapid amplification of cDNA ends; Vibrio anguillarum; gene expression; amino acids; hepatopancreas; Edwardsiella tarda; tissues; Escherichia coli; messenger RNA; Argopecten irradians; lactose; complementary DNA; muscles; immunologic receptors; amino acid sequences; encapsulation; immune response; kidneys; Vibrio fluvialis; innate immunity; galactose; lectins; vertebrates; expressed sequence tags; agarose; scallops; hemocytes
Abstract:
... Galectins are a family of β-galactoside-binding lectins that specifically bind to β-galactoside residues and play crucial roles in innate immune responses of invertebrates and vertebrates. The cDNA of bay scallop Argopecten irradians galectin (designated as AiGal2) was cloned by rapid amplification of cDNA ends (RACE) method based on the expressed sequence tag (EST). The full-length cDNA of AiGal2 ...
DOI:
10.1016/j.dci.2011.01.006

14. Age-associated methylation change of TAP1 promoter in piglet

Author:
Dong, Wenhua; Yin, Xuemei; Sun, Li; Wang, Jing; Sun, Shouyong; Zhu, Guoqiang; Wu, Shenglong; Bao, Wenbin
Source:
Gene 2015 v.573 pp. 70-74
ISSN:
0378-1119
Subject:
Escherichia coli; analysis of variance; antigens; binding sites; diarrhea; financial economics; fluorescence; gene expression; gene expression regulation; genes; genomic islands; immune response; jejunum; messenger RNA; methylation; mortality; mutation; piglets; pork industry; promoter regions; quantitative polymerase chain reaction; sequence analysis; transcription factors; weaning
Abstract:
... Diarrhea and edematous disease are two major causes of mortality in postweaning piglets. These conditions lead to huge economic losses in the swine industry. Escherichia coli F18 is the primary causative agent of these two diseases. Transported associated with antigen processing (TAP) plays an important role in the immune response and the TAP1 gene could be an effective anti-E. coli F18 molecular ...
DOI:
10.1016/j.gene.2015.07.026

15. A glutathione S-transferase from Proteus mirabilis involved in heavy metal resistance and its potential application in removal of Hg²⁺

Author:
Zhang, Weiwei; Yin, Kun; Li, Bowei; Chen, Lingxin
Source:
Journal of hazardous materials 2013 v.261 pp. 646-652
ISSN:
0304-3894
Subject:
DNA; Escherichia coli; Proteus mirabilis; aquatic environment; bacteria; gene expression; genes; glutathione transferase; mercuric chloride; mercury; messenger RNA; metal tolerance; molecular weight; pH; proteins; reverse transcriptase polymerase chain reaction
Abstract:
... Glutathione S-transferases (GSTs) are a family of multifunctional proteins playing important roles in detoxification of harmful physiological and xenobiotic compounds in organisms. In our study, a gene encoding a GST from Proteus mirabilis strain V7, gstPₘ-4, was cloned and conditionally expressed in Escherichia coli strain BL21(DE3). The purified GstPₘ-4 protein, with an estimated molecular mass ...
DOI:
10.1016/j.jhazmat.2013.08.023

16. A GTP-dependent Phosphoenolpyruvate Carboxykinase from Crassostrea gigas Involved in Immune Recognition

Author:
Lv, Zhao; Qiu, Limei; Wang, Weilin; Liu, Zhaoqun; Xue, Zhuang; Yu, Zichao; Song, Xiaorui; Chen, Hao; Wang, Lingling; Song, Linsheng
Source:
Developmental and comparative immunology 2017 v.77 pp. 318-329
ISSN:
0145-305X
Subject:
Crassostrea gigas; Escherichia coli; Micrococcus luteus; Staphylococcus aureus; Vibrio splendidus; active sites; bacteria; beta-glucans; binding sites; biochemical pathways; gene expression; gluconeogenesis; guanosine triphosphate; hemocytes; hepatopancreas; immune response; immunohistochemistry; lipopolysaccharides; lipoteichoic acids; messenger RNA; muscles; oysters; peptidoglycans; proteins; tissues
Abstract:
... Phosphoenolpyruvate carboxykinase (PEPCK) is well known as a key enzyme involved in the metabolic pathway of gluconeogenesis in organisms, but the information about its involvement in immune response is still very limited. In the present study, a novel PEPCK homolog named CgPEPCK was identified from oyster Crassostrea gigas. The deduced amino acid sequence of CgPEPCK shared 52%–74% similarities wi ...
DOI:
10.1016/j.dci.2017.09.001

17. A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii

Author:
Xin-peng, Zhao; Yong-hua, Hu; Yong, Liu; Jing-jing, Wang; Guang-hua, Wang; Ren-jie, Wang; Min, Zhang
Source:
Fish & shellfish immunology 2016 v.56 pp. 402-409
ISSN:
1050-4648
Subject:
Sebastes schlegelii; fish; nucleoproteins; dose response; open reading frames; DNA; brain; macrophages; Vibrio anguillarum; gene expression; bacterial infections; pathogens; Escherichia coli; tissues; messenger RNA; invertebrates; amino acid sequences; tumor necrosis factor-alpha; kidneys; interleukin-1beta; Anoplopoma fimbria; antibacterial properties; DNA fragmentation; bacteria
Abstract:
... High-mobility group box (HMGB) 1 is a chromosomal protein that plays critical roles in DNA transcription, replication and repair. In addition, HMGB1 functions as a pro-inflammatory molecule in many vertebrates and invertebrates. In teleosts, very limited studies of HMGB1 have been reported. In this study, we identified a HMGB1 homologue (SsHMGB1) from black rockfish (Sebastes schlegelii) and analy ...
DOI:
10.1016/j.fsi.2016.07.034

18. AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain

Author:
Zhang, Huan; Song, Xiaoyan; Wang, Lingling; Kong, Pengfei; Yang, Jialong; Liu, Lin; Qiu, Limei; Zhang, Ying; Qiu, Lihua; Song, Linsheng
Source:
Fish & shellfish immunology 2011 v.30 no.1 pp. 17-26
ISSN:
1050-4648
Subject:
Staphylococcus aureus; Gram-positive bacteria; polypeptides; disulfide bonds; Micrococcus luteus; open reading frames; recombinant fusion proteins; tissue distribution; rapid amplification of cDNA ends; Vibrio anguillarum; gene expression; hepatopancreas; disease resistance; signal peptide; gene expression regulation; cysteine; Escherichia coli; Gram-negative bacteria; messenger RNA; Argopecten irradians; complementary DNA; conserved sequences; molecular cloning; antibacterial properties; quantitative polymerase chain reaction; innate immunity; lectins; expressed sequence tags; scallops
Abstract:
... C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, a novel C-type lectin gene from scallop Argopecten irradians (designated as AiCTL-6) was cloned by rapid amplification of cDNA ends (RACE) approach based on expression sequence tag (EST) analysis. The full-length cDNA of AiCTL-6 was 1080 bp. The open reading frame e ...
DOI:
10.1016/j.fsi.2009.12.019
PubMed:
20045059

19. Algae-derived β-glucan enhanced gut health and immune responses of weaned pigs experimentally infected with a pathogenic E. coli

Author:
Kim, Kwangwook; Ehrlich, Amy; Perng, Vivian; Chase, Jennifer A.; Raybould, Helen; Li, Xunde; Atwill, Edward R.; Whelan, Rose; Sokale, Adebayo; Liu, Yanhong
Source:
Animal feed science and technology 2019 v.248 pp. 114-125
ISSN:
0377-8401
Subject:
Escherichia coli; absorption barrier; beta-glucans; blood sampling; blood serum; cortisol; diarrhea; feed supplements; gene expression; gene expression regulation; genes; haptoglobins; heat stability; ileum; immune response; inoculum; interleukin-6; jejunum; messenger RNA; mucosa; neutrophils; occludins; oral administration; permeability; swine; tumor necrosis factor-alpha; yeasts
Abstract:
... Most of the commercially available β-glucans are derived from yeast, while there are limited research on algae-derived β-glucan in pigs. Therefore, the objective of this experiment was to investigate the influence of dietary supplementation of algae-derived β-glucan on diarrhea, gut permeability, and immune responses of weaned pigs experimentally infected with a pathogenic Escherichia coli (E. col ...
DOI:
10.1016/j.anifeedsci.2018.12.004

20. Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

Author:
Pi, Yan; Jiang, Keji; Cao, Ying; Wang, Qian; Huang, Zhuoshi; Li, Le; Hu, Lingchuan; Li, Wei; Sun, Xiaofen; Tang, Kexuan
Source:
Molecular biotechnology 2009 v.41 no.2 pp. 115-122
ISSN:
1073-6085
Subject:
Agrobacterium radiobacter; Camptotheca acuminata; Escherichia coli; Nicotiana tabacum; bacteria; biochemical pathways; chlorophyll; cold stress; cold tolerance; electrolytes; gene expression; genes; genetic engineering; jasmonic acid; messenger RNA; quantitative polymerase chain reaction; salt stress; sodium chloride; temperature; tobacco; transgenic plants; wild plants
Abstract:
... Allene oxide cyclase (AOC, E 5.3.99.6) is an essential enzyme in jasmonate (JA) biosynthetic pathway. An AOC gene (defined as CaAOC, Database Accession No. AY863428) had been isolated from Camptotheca acuminata in previous work. Real-time quantitative PCR analysis indicated that mRNA expression of CaAOC was induced by salt stress (120 mM NaCl) and low temperature (4°C). In order to further investi ...
DOI:
10.1007/s12033-008-9106-z