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You searched for: Subject term microsatellite repeats Remove constraint Subject term: microsatellite repeats Subject term forensic sciences Remove constraint Subject term: forensic sciences
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1. A BSA coated NOA81 PCR chip for gene amplification

Author:
Kuiwei Qin; Xuefei Lv; Qiaorui Xing; Rui Li; Yulin Deng
Source:
Analytical methods 2016 v.8 no.12 pp. 2584-2591
ISSN:
1759-9679
Subject:
analytical methods; bovine serum albumin; coatings; cobalt; forensic sciences; gene amplification; genes; microsatellite repeats; mutation; organ-on-a-chip; pH; point-of-care testing; polymerase chain reaction; temperature
Abstract:
... Norland Optical Adhesive 81 (NOA81) is an excellent material for a microfluidic chip, but this chip has been rarely used in the field of gene amplification due to the inhibition effect on the polymerase chain reaction (PCR). In this study, a NOA81 PCR chip (NP-chip) was developed with a simple, fast fabrication method. To overcome this inhibition effect, a simple bovine serum albumin (BSA) coating ...
DOI:
10.1039/c5ay03233d

2. A Forensic Genomics Approach for the Identification of Sister Marija Crucifiksa Kozulić

Author:
Charla Marshall; Kimberly Sturk-Andreaggi; Erin M. Gorden; Jennifer Daniels-Higginbotham; Sidney Gaston Sanchez; Željana Bašić; Ivana Kružić; Šimun Anđelinović; Alan Bosnar; Miran Čoklo; Anja Petaros; Timothy P. McMahon; Dragan Primorac; Mitchell M. Holland
Source:
Genes 2020 v.11 no.8 pp. -
ISSN:
2073-4425
Subject:
bones; forensic sciences; genomics; haplotypes; kinship; microsatellite repeats; mitochondria; mitochondrial genome; sequence analysis; single nucleotide polymorphism; Croatia; Vatican
Abstract:
... Sister Marija Krucifiksa Kozulić (1852–1922) was a Croatian nun who is in consideration for beatification by the Vatican, which is facilitated by the identification of her 20th-century remains. Sister Marija was buried in a tomb in Rijeka, Croatia, along with other nuns including her biological sister, Tereza Kozulić (1861–1933). When the remains were exhumed in 2011, they were found in a deterior ...
DOI:
10.3390/genes11080938

3. A SNaPshot assay for genotyping 44 individual identification single nucleotide polymorphisms

Author:
Lou, Chunguang; Cong, Bin; Li, Shujin; Fu, Lihong; Zhang, Xiaojing; Feng, Ting; Su, Shanshan; Ma, Chunling; Yu, Feng; Ye, Jian; Pei, Li
Source:
Electrophoresis 2011 v.32 no.3-4 pp. 368-378
ISSN:
0173-0835
Subject:
DNA; DNA primers; electrophoresis; fluorescence; forensic sciences; genotyping; heterozygosity; humans; loci; microsatellite repeats; paternity; polymerase chain reaction; single nucleotide polymorphism
Abstract:
... Single nucleotide polymorphisms (SNPs), which have relatively low mutation rates and can be genotyped after PCR with shorter amplicons compared with short tandem repeats (STRs), are being considered as potentially useful markers in forensic DNA analysis. Those SNPs with high heterozygosity and low Fst (F-statistics) in human populations are described as individual identification SNPs, which perfor ...
DOI:
10.1002/elps.201000426

4. A highly divergent microsatellite facilitating fast and accurate DRB haplotyping in humans and rhesus macaques

Author:
Doxiadis, Gaby G.M.; de Groot, Nanine; Claas, Frans H.J.; Doxiadis, Ilias I.N.; van Rood, Jon J.; Bontrop, Ronald E.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.21 pp. 8907-8912
ISSN:
0027-8424
Subject:
microsatellite repeats; paternity; haplotypes; humans; medicine; phylogeny; Macaca mulatta; genotyping; forensic sciences; exons
Abstract:
... The DRB region of the MHC in primate species is known to display abundant region configuration polymorphism with regard to the number and content of genes present per haplotype. Furthermore, depending on the species studied, the different DRB genes themselves may display varying degrees of allelic polymorphism. Because of this combination of diversity (differential gene number) and polymorphism (a ...
DOI:
10.1073/pnas.0702964104
PubMed:
17502594
PubMed Central:
PMC1868589

5. A microhap panel for kinship analysis through massively parallel sequencing technology

Author:
Ning Qu; Shaobin Lin; Yu Gao; Hao Liang; Hu Zhao; Xueling Ou
Source:
Electrophoresis 2020 v.41 no.3-4 pp. 246-253
ISSN:
0173-0835
Subject:
Chinese people; alleles; electrophoresis; forensic sciences; genotyping; haplotypes; heterozygosity; high-throughput nucleotide sequencing; kinship; loci; microsatellite repeats; mutation rate; single nucleotide polymorphism
Abstract:
... It is widely recognized that microhaps are powerful markers for different forensic purposes, mainly due to their advantages of both short tandem repeats and single nucleotide polymorphisms, including multiple alleles, low mutation rate, and absence of stutter peaks. In the present study, a panel of 60 microhap loci was developed and utilized in forensic kinship analysis as a preliminary study. Gen ...
DOI:
10.1002/elps.201900337

6. A new approach combining forensic thresholds and a multiple-tubes approach to unravel false microsatellite profiles from cross-contaminated sample material

Author:
Kathrin Mäck; Andreas Scharbert; Ralf Schulz; René Sahm
Source:
Conservation genetics resources 2021 v.13 no.1 pp. 89-95
ISSN:
1877-7252
Subject:
Alosa alosa; DNA; conservation genetics; cross contamination; forensic sciences; genotyping; microsatellite repeats; scales (integument)
Abstract:
... Contamination and degradation are known challenges for reliable genotyping, since they can cause, among other problems, false microsatellite profiles. In this study we described a method to decrease the proportion of false microsatellite profiles from fish scale samples of endangered allis shads (Alosa alosa) from a reintroduction program, where cross-contamination with DNA from other individuals ...
DOI:
10.1007/s12686-020-01175-3

7. A novel 13‐plex STR typing system for individual identification and parentage testing of donkeys (Equus asinus)

Author:
W. Dang; S. Shang; X. Zhang; Y. Yu; D. M. Irwin; Z. Wang; S. Zhang
Source:
Equine veterinary journal 2020 v.52 no.2 pp. 290-297
ISSN:
0425-1644
Subject:
DNA; Equus asinus; asses; fluorescent dyes; forensic sciences; genetic analysis; genetic profile; genetic variation; hinnies; horses; hybrids; microsatellite repeats; mules; null alleles; paternity; polymerase chain reaction; population genetics
Abstract:
... BACKGROUND: Previous studies investigating donkey parentage and genetic diversity used horse‐specific multiplex systems. However, several mis‐allele and null‐allele issues were found with some of the horse primers when used in donkeys. In 2017, the International Society for Animal Genetics (ISAG) recommended 13 dinucleotide short tandem repeats (STRs) (AHT4, ASB23, HMS2, HMS3, HMS6, HMS7, HMS18, H ...
DOI:
10.1111/evj.13158

8. A proposal for standardization in forensic bovine DNA typing: allele nomenclature of 16 cattle-specific short tandem repeat loci

Author:
van de Goor, L.H.P.; Panneman, H.; van Haeringen, W.A.
Source:
Animal genetics 2009 v.40 no.5 pp. 630-636
ISSN:
0268-9146
Subject:
DNA; DNA fingerprinting; alleles; cattle; forensic sciences; genotyping; homozygosity; loci; microsatellite repeats
Abstract:
... In this study, a proposal is presented for the allele nomenclature of 16 polymorphic short tandem repeat (STR) loci (BM1824, BM2113, ETH10, ETH225, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH3, TGLA53, BM1818, CSRM60, CSSM66, HAUT27 and ILSTS006) for bovine genotyping (Bos taurus). The nomenclature is based on sequence data of the polymorphic region(s) of the STR loci as recommended by the DNA ...
DOI:
10.1111/j.1365-2052.2009.01891.x
PubMed:
19397508

9. A rapid nuclear staining test using cationic dyes contributes to efficient STR analysis of telogen hair roots

Author:
So‐Yeon Lee; Eun‐Ju Ha; Seung‐Kyun Woo; So‐Min Lee; Kyung‐Hee Lim; Yong‐Bin Eom
Source:
Electrophoresis 2017 v.38 no.13-14 pp. 1771-1779
ISSN:
0173-0835
Subject:
DNA fingerprinting; adverse effects; alleles; cost effectiveness; electrophoresis; forensic sciences; hairs; head; methylene blue; microsatellite repeats; microscopy; nuclear genome; screening; staining; toluidine blue
Abstract:
... Telogen hairs presented in the crime scene are commonly encountered as trace evidence. However, short tandem repeat (STR) profiling of the hairs currently have low and limited use due to poor success rate. To increase the success rate of STR profiling of telogen hairs, we developed a rapid and cost‐effective method to estimate the number of nuclei in the hair roots. Five cationic dyes, Methyl gree ...
DOI:
10.1002/elps.201700024

10. A rapid screening method using DNA binding dyes to determine whether hair follicles have sufficient DNA for successful profiling

Author:
Alicia M. Haines; Adrian Linacre
Source:
Forensic science international 2016 v.262 pp. 190-195
ISSN:
0379-0738
Subject:
DNA; DNA fingerprinting; alleles; databases; females; fluorescence; forensic sciences; hair follicles; hairs; loci; males; microsatellite repeats; polymerase chain reaction; prediction; rapid methods; root tips; screening; viability
Abstract:
... We report a simple screening method to assess the viability of successful DNA profiling from single hair follicles. A total of 48 hair samples (shed and plucked) were collected from male and female donors and the root tips (0.5cm) were stained using one of three DNA binding dyes (EvaGreen™, Diamond™ Nucleic Acid Dye and RedSafe™) at 20× concentration. The hairs were subsequently viewed under a Nik ...
DOI:
10.1016/j.forsciint.2016.03.026

11. A survey of the effects of common illicit drugs on forensic DNA analysis

Author:
Amy Griffin; Julianne Henry; K. Paul Kirkbride; Ben Painter; Adrian Linacre
Source:
Forensic science international 2022 v.336 pp. 111314
ISSN:
0379-0738
Subject:
DNA; alleles; fluorescence; forensic sciences; illicit drugs; law enforcement; loci; manufacturing; microsatellite repeats; quantitative polymerase chain reaction; surveys
Abstract:
... Profiling of DNA associated with illicit drug packages and paraphernalia is a common investigative tool. In addition, research is being conducted regarding the analysis of trace DNA present within illicit drugs and on capsules. The application of trace DNA analysis to illicit drugs has the potential to identify individuals involved in their manufacture and distribution. However, the inhibitory eff ...
DOI:
10.1016/j.forsciint.2022.111314

12. Aged fingerprints for DNA profile: First report of successful typing

Author:
Carlo Giovanni Romano; Rosaria Mangiaracina; Luigi Donato; Rosalia D’Angelo; Concetta Scimone; Antonina Sidoti
Source:
Forensic science international 2019 v.302 pp. 109905
ISSN:
0379-0738
Subject:
DNA; crime; databases; forensic sciences; microsatellite repeats; powders; storage conditions
Abstract:
... Fingerprint detection and Short Tandem Repeat (STR) typing are two approaches used for identification of individuals. The main goal of forensic laboratories is the development of a standardized protocol to obtain an STR profile from latent fingerprints, by typing the DNA transferred onto touched objects. The results obtained in this field derive from studies conducted under controlled laboratory c ...
DOI:
10.1016/j.forsciint.2019.109905

13. Allele and haplotype diversity of new multiplex of 19 ChrX‐STR loci in Han population from Guanzhong region (China)

Author:
Yu‐Dang Zhang; Chun‐Mei Shen; Hao‐Tian Meng; Yu‐Xin Guo; Qian Dong; Guang Yang; Jiang‐Wei Yan; Yao‐Shun Liu; Ting Mei; Rui‐Zhe Huang; Bo‐Feng Zhu
Source:
Electrophoresis 2016 v.37 no.12 pp. 1669-1675
ISSN:
0173-0835
Subject:
alleles; electrophoresis; females; forensic sciences; genetic polymorphism; haplotypes; loci; males; microsatellite repeats; paternity; Africa; China; Europe; North America
Abstract:
... X‐chromosomal short tandem repeats (X‐STRs) have been proved to be useful for some deficiency paternity cases in recent years. Here, we studied the genetic polymorphisms of 19 X‐STR loci (DXS10148‐DXS10135‐DXS8378, DXS10159‐DXS10162‐DXS10164, DXS7132‐DXS10079‐DXS10074‐DXS10075, DXS6809‐DXS6789, DXS7424‐DXS101, DXS10103‐HPRTB‐DXS10101 and DXS7423‐DXS10134) in 252 male and 222 female individuals fro ...
DOI:
10.1002/elps.201500425

14. Allele frequencies of 23 short tandem repeat loci in the Vietnamese Kinh population

Author:
Thi Thanh Hoan Vu; Thi Thu Mai Do; Thi Hoai Nguyen; Quoc Hai Luyen
Source:
Forensic science international 2021 v.3 pp. 100210
ISSN:
2665-9107
Subject:
alleles; capillary electrophoresis; data collection; databases; forensic sciences; gene frequency; genetic polymorphism; humans; loci; microsatellite repeats; paternity; quality control
Abstract:
... The analysis of short tandem repeat (STR) loci using PCR followed by capillary electrophoresis (CE) remains the gold standard in forensic genetics and human identity testing. However, the quality of the STR allele frequency databases for Vietnamese Kinh population is not assured and the number of investigated STR loci in the researches is limited. To increase the reliability of allele frequency da ...
DOI:
10.1016/j.fsir.2021.100210

15. Alternatives to amelogenin markers for sex determination in humans and their forensic relevance

Author:
Hirak R. Dash; Neha Rawat; Surajit Das
Source:
Molecular biology reports 2020 v.47 no.3 pp. 2347-2360
ISSN:
0301-4851
Subject:
DNA; Y chromosome; forensic sciences; genes; microsatellite repeats; molecular biology; sex determination; tooth enamel
Abstract:
... Forensic DNA typing and subsequent molecular methods of sex determination in humans have been proven to be an imperious tool to criminal justice system. In current practice, most of the short tandem repeat (STR) based commercial kits contain amelogenin as the sexing marker. Amelogenin gene which contributes to the tooth enamel formation is present on both X and Y chromosome with a variation in bas ...
DOI:
10.1007/s11033-020-05268-y

16. An accurate and rapid method for species identification in plants: Melting fingerprint-high resolution melting (MFin-HRM) analysis

Author:
Kittisak Buddhachat; Phanupong Changtor; Sunatcha Ninket
Source:
Plant gene 2019 pp. -
ISSN:
2352-4073
Subject:
DNA; DNA fingerprinting; Phyllanthus amarus; adulterants; ecology; forensic sciences; genes; industry; melting; microsatellite repeats; models; pharmacology; rapid methods; species identification
Abstract:
... Species identification is required in various areas such as systematics, ecology, conservation, evolution, agriculture, forensics, pharmacology, food science, and even industry. Yet the number of taxonomists who identify based on morphological observations is limited, and some materials are incomplete and/or processed, leading to difficulty in species identification by morphological features. Thus ...
DOI:
10.1016/j.plgene.2019.100203

17. Analysis of 12 X-STR loci in the population of south Croatia

Author:
Gordan Mršić; Petar Ozretić; Josip Crnjac; Siniša Merkaš; Ivana Račić; Sara Rožić; Viktorija Sukser; Maja Popović; Marina Korolija
Source:
Molecular biology reports 2017 v.44 no.1 pp. 183-189
ISSN:
0301-4851
Subject:
DNA; X chromosome; capillary electrophoresis; computer software; females; forensic sciences; haplotypes; linkage disequilibrium; linkage groups; loci; males; microsatellite repeats; polymerase chain reaction; Croatia
Abstract:
... The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator® Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic ...
DOI:
10.1007/s11033-017-4096-1

18. Analysis of DNA profiles of ash (Fraxinus excelsior L.) to provide evidence of illegal logging

Author:
Anna Tereba; Stephen Woodward; Agata Konecka; Małgorzata Borys; Justyna Anna Nowakowska
Source:
Wood science and technology 2017 v.51 no.6 pp. 1377-1387
ISSN:
0043-7719
Subject:
DNA; DNA fingerprinting; Fraxinus excelsior; chloroplasts; forensic sciences; genetic markers; logging; microsatellite repeats; molecular biology; monitoring; probability; state forests; wood; Europe
Abstract:
... Illegal logging is a major problem in many European countries. Recent progress in molecular biology, however, has significantly improved the ability to accurately identify wood material. In this paper, the first application of microsatellite DNA markers is described in a case of illegal logging of European ash wood in Polish State Forests. The genetic fingerprints of seized ash wood samples were d ...
DOI:
10.1007/s00226-017-0942-5

19. Analysis of TPOX short tandem repeat locus with matrix-associated laser desorption/ionization time-of-flight-based restriction fragment mass polymorphism assay

Author:
Yoon Seok Cha; Suh Hee Choi; Joo-Hyoung Lee; Soo-Kyung Shin; Seung Hwan Lee; Soong Deok Lee; Soo-Ok Kim; Sun Pyo Hong
Source:
Analytical biochemistry 2011 v.412 no.1 pp. 79-84
ISSN:
0003-2697
Subject:
DNA fragmentation; capillary electrophoresis; desorption; forensic sciences; genetic disorders; genotype; genotyping; ionization; loci; matrix-assisted laser desorption-ionization mass spectrometry; microsatellite repeats; paternity; polymerase chain reaction; restriction endonucleases; sequence analysis
Abstract:
... Short tandem repeat (STR) loci are routinely analyzed by capillary electrophoresis. However, this method has several disadvantages, including long operational time, low throughput, and inaccuracy. As a result of the introduction of matrix-associated laser desorption/ionization time-of-flight (MALDI–TOF) and electrospray ionization (ESI), mass spectrometry has become an alternative method for genot ...
DOI:
10.1016/j.ab.2011.01.023

20. Animal Forensic Genetics

Author:
Adrian Linacre
Source:
Genes 2021 v.12 no.4 pp. -
ISSN:
2073-4425
Subject:
DNA; dogs; forensic sciences; humans; loci; microsatellite repeats; mitochondria; mitochondrial genome; pets; protected species; species identification; trade; wildlife
Abstract:
... Animal forensic genetics, where the focus is on non-human species, is broadly divided in two: domestic species and wildlife. When traces of a domestic species are relevant to a forensic investigation the question of species identification is less important, as the material comes from either a dog or a cat for instance, but more relevant may be the identification of the actual pet. Identification o ...
DOI:
10.3390/genes12040515