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You searched for: Author "Saisuk, W." Remove constraint Author: "Saisuk, W." Subject nucleic acid hybridization Remove constraint Subject: nucleic acid hybridization Subject RNA Remove constraint Subject: RNA
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1. Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses

Author:
Saisuk, W.,   et al.   ; Srisawat, C.; Yoksan, S.; Dharakul, T.;     Show all 4 Authors
Source:
Analytical chemistry 2019 v.91 no.5 pp. 3286-3293
ISSN:
1520-6882
Subject:
RNA; dengue; detection limit; nucleic acid hybridization; reverse transcription; temperature; viruses
Abstract:
... Biological RNA generally comprises secondary structure motifs which cause a problem for target RNA detection by isothermal amplification methods. The complexity of the secondary structures makes RNA targets inaccessible for probe hybridization, resulting in decreased sensitivity and selectivity. This is particularly important because the hybridization step of the isothermal amplification method re ...
DOI:
10.1021/acs.analchem.8b03736