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1. The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase

Author:
Bollella, Paolo; Hibino, Yuya; Kano, Kenji; Gorton, Lo; Antiochia, Riccarda
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.14 pp. 3253-3264
ISSN:
1618-2642
Subject:
calcium chloride,   etc   ; absorbance; active sites; aspartic acid; calcium; carbon electrodes; catalytic activity; cations; chelation; circular dichroism spectroscopy; cytochrome c; electron transfer; enzyme activity; fructose; glutamic acid; heme; magnesium chloride; oxidation; pH; potassium; potassium chloride; sodium; sodium chloride;     Show all 23 Subjects
Abstract:
... We report on the influence of pH and monovalent/divalent cations on the catalytic current response, internal electron transfer (IET), and structure of fructose dehydrogenase (FDH) by using amperometry, spectrophotometry, and circular dichroism (CD). Amperometric measurements were performed on graphite electrodes, onto which FDH was adsorbed and the effect on the response current to fructose was in ...
DOI:
10.1007/s00216-018-0991-0
PubMed:
29564502
PubMed Central:
PMC5937911

2. Quality of Cucumbers Commercially Fermented in Calcium Chloride Brine without Sodium Salts

Author:
McMurtrie, Erin K.; Johanningsmeier, Suzanne D.
Source:
Journal of food quality 2018 v.2018
ISSN:
1745-4557
Subject:
calcium chloride,   etc   ; brining; calcium; color; cucumbers; firmness; lactic fermentation; mesocarp; pH; photooxidation; potassium sorbate; product quality; seasonal variation; sodium; sodium chloride; starter cultures; storage time; tanks; texture; wastewater;     Show all 20 Subjects
Abstract:
... Commercial cucumber fermentation produces large volumes of salty wastewater. This study evaluated the quality of fermented cucumbers produced commercially using an alternative calcium chloride (CaCl₂) brining process. Fermentation conducted in calcium brines (0.1 M CaCl₂, 6 mM potassium sorbate, equilibrated) with a starter culture was compared to standard industrial fermentation. Production varia ...
Handle:
10113/6240209
DOI:
10.1155/2018/8051435
Chorus Open Access:
10.1155/2018/8051435

3. Probing the Surface Charge on the Basal Planes of Kaolinite Particles with High-Resolution Atomic Force Microscopy

Author:
Kumar, N.; Andersson, M. P.; van den Ende, D.; Mugele, F.; Siretanu, I.
Source:
Langmuir 2017 v.33 no.50 pp. 14226-14237
ISSN:
1520-5827
Subject:
calcium chloride,   etc   ; adsorption; atomic force microscopy; calcium; density functional theory; gibbsite; image analysis; ions; kaolinite; nanoparticles; neutralization; pH; salt concentration; silica; sodium chloride;     Show all 15 Subjects
Abstract:
... High-resolution atomic force microscopy is used to map the surface charge on the basal planes of kaolinite nanoparticles in an ambient solution of variable pH and NaCl or CaCl₂ concentration. Using DLVO theory with charge regulation, we determine from the measured force–distance curves the surface charge distribution on both the silica-like and the gibbsite-like basal plane of the kaolinite partic ...
DOI:
10.1021/acs.langmuir.7b03153
PubMed:
29140711
PubMed Central:
PMC5738629

4. Soil pretreatment and fast cell lysis for direct polymerase chain reaction from forest soils for terminal restriction fragment length polymorphism analysis of fungal communities

Author:
Cheng, Fei; Hou, Lin; Woeste, Keith; Shang, Zhengchun; Peng, Xiaobang; Zhao, Peng; Zhang, Shuoxin
Source:
Brazilian journal of microbiology 2016 v.47 no.4 pp. 817-827
ISSN:
1517-8382
Subject:
calcium chloride,   etc   ; DNA; calcium; community structure; flocculation; forest soils; fungal communities; fungi; glass; humic substances; internal transcribed spacers; octoxynol; pH; polymerase chain reaction; polyvinylpyrrolidone; restriction fragment length polymorphism; skim milk; sodium chloride; soil biota; soil pollution; soil sampling; transcription (genetics);     Show all 22 Subjects
Abstract:
... Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, ...
DOI:
10.1016/j.bjm.2016.06.007
PubMed:
27528083
PubMed Central:
PMC5052337

5. Effect of Lipid Headgroup Charge and pH on the Stability and Membrane Insertion Potential of Calcium Condensed Gene Complexes

Author:
Alhakamy, Nabil A.; Elandaloussi, Ibrahim; Ghazvini, Saba; Berkland, Cory J.; Dhar, Prajnaparamita
Source:
Langmuir 2015 v.31 no.14 pp. 4232-4245
ISSN:
1520-5827
Subject:
calcium chloride,   etc   ; acidity; adsorption; calcium; cell membranes; chemical composition; endocytosis; endosomes; genes; hydrodynamics; hydrophilicity; models; pH; peptides; phospholipids; small interfering RNA; surfactants; transfection; zwitterions;     Show all 19 Subjects
Abstract:
... Noncovalently condensed complexes of genetic material, cell penetrating peptides (CPPs), and calcium chloride present a nonviral route to improve transfection efficiency of nucleic acids (e.g., pDNA and siRNA). However, the exact mechanisms of membrane insertion and delivery of macromolecule complexes to intracellular locations as well as their stability in the intracellular environment are not un ...
DOI:
10.1021/la504970n
PubMed:
25768428
PubMed Central:
PMC5704962

6. Casein maps: Effect of ethanol, pH, temperature, and CaCl2 on the particle size of reconstituted casein micelles

Author:
Ye, Ran; Harte, Federico
Source:
Journal of dairy science 2013 v.96 no.2 pp. 799-805
ISSN:
0022-0302
Subject:
calcium chloride,   etc   ; calcium; casein; dissociation; ethanol; light scattering; micelles; pH; particle size; temperature;     Show all 10 Subjects
Abstract:
... Although conditions favoring casein micelle aggregation are well known, factors promoting the dissociation of the casein micelle are not fully understood. It was our objective to investigate the ethanol-induced dissociation of micellar casein as affected by temperature and a wide range of pH, along with the concentrations of calcium and casein. Two different concentrations of casein micelles were ...
DOI:
10.3168/jds.2012-5838
PubMed:
23200467
PubMed Central:
PMC4559271

7. Ionic and pH effects on the osmotic properties and structure of polyelectrolyte gels

Author:
Horkay, Ferenc; Basser, Peter J.
Source:
Journal of polymer science 2008 v.46 no.24 pp. 2803-2810
ISSN:
0887-6266
Subject:
calcium chloride,   etc   ; neutralization; polyacrylic acid; ions; calcium; osmotic pressure; acrylic acid; gels; pH; salt concentration; sodium chloride;     Show all 11 Subjects
Abstract:
... We investigate the effects of salt concentration and pH on neutralized poly(acrylic acid) (PAA) gels in near physiological salt solutions. Either adding calcium ions or decreasing the pH is found to induce reversible volume transitions but the nature of these transitions seems to be different. For example, the osmotic pressure exhibits a simple power law dependence on the concentration as the tran ...
DOI:
10.1002/polb.21590
PubMed:
20016687
PubMed Central:
PMC2782841