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1. Mutagenesis of N-terminal residues confer thermostability on a Penicillium janthinellum MA21601 xylanase

Author:
Fan, Guangsen,   et al.   ; Xiong, Ke; Hou, Jie; Jiang, Yuefeng; Li, Xiuting; Teng, Chao; Li, Qin; Yang, Ran; Zhang, Chengnan;     Show all 9 Authors
Source:
BMC biotechnology 2019 v.19 no.1 pp. 51
ISSN:
1472-6750
Subject:
Penicillium janthinellum; disulfide bonds; feed industry; feeds; half life; hydrophobicity; mutagenesis; mutants; pH; thermal stability; xylanases
Abstract:
... BACKGROUND: A mesophilic xylanase PjxA from Penicillium janthinellum MA21601 has high specific activity under acidic condition and holds great potential for applications in the animal feed industry. To enhance the thermostability of xylanase PjxA, two mutation strategies in the N-terminal region were examined and then integrated into the xylanase to further improvement. The recombinant xylanase PT ...
DOI:
10.1186/s12896-019-0541-7

2. Screening, purification and characterization of lipase from Burkholderia pyrrocinia B1213

Author:
Fan, Guangsen,   et al.   ; Li, Jinlong; Shen, Weijia; Li, Xiuting;     Show all 4 Authors
Source:
3 Biotech 2018 v.8 no.9 pp. 387
ISSN:
2190-572X
Subject:
Burkholderia pyrrocinia; acetone; aluminum; carboxylic ester hydrolases; chromatography; enzyme activity; filtration; genes; hexane; iron; isopropyl alcohol; magnesium; manganese; moieties; pH; ribosomal RNA; screening; soil; temperature
Abstract:
... A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia. Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35 ...
DOI:
10.1007/s13205-018-1414-9

3. Systematic Characterization of the Metabolism of Acetoin and Its Derivative Ligustrazine in Bacillus subtilis under Micro-Oxygen Conditions

Author:
Fan, Guangsen,   et al.   ; Xu, Youqiang; Jiang, Yuefeng; Li, Xiuting; Sun, Baoguo; Teng, Chao; Yang, Ran; Xiong, Ke; Wang, Wenhua;     Show all 9 Authors
Source:
Journal of agricultural and food chemistry 2018 v.66 no.12 pp. 3179-3187
ISSN:
1520-5118
Subject:
Bacillus subtilis; Vigna angularis; acetoin; brewing; culture media; diammonium phosphate; fermentation; flavor; gene overexpression; genes; glucose; mutation; pH
Abstract:
... Bacillus subtilis is an important microorganism for brewing of Chinese Baijiu, which contributes to the formation of flavor chemicals including acetoin and its derivative ligustrazine. The first stage of Baijiu brewing process is under micro-oxygen conditions; however, there are few studies about B. subtilis metabolism under these conditions. Effects of various factors on acetoin and ligustrazine ...
DOI:
10.1021/acs.jafc.8b00113

4. Characterization of a highly thermostable glycoside hydrolase family 10 xylanase from Malbranchea cinnamomea

Author:
Fan, Guangsen,   et al.   ; Yang, Shaoqing; Yan, Qiaojuan; Guo, Yu; Li, Yanxiao; Jiang, Zhengqiang;     Show all 6 Authors
Source:
International journal of biological macromolecules 2014 v.70 pp. 482-489
ISSN:
0141-8130
Subject:
thermal stability; xylooligosaccharides; xylan; open reading frames; xylanases; polyacrylamide gel electrophoresis; Aureobasidium pullulans; polymerization; amino acid sequences; thermophilic fungi; pH; temperature; molecular weight; Malbranchea; glycosides; introns; half life
Abstract:
... A thermostable xylanase (McXyn10) from the thermophilic fungus Malbranchea cinnamomea strain S168 was purified and biochemically characterized. The enzyme was purified to homogeneity with a molecular mass of 43.5kDa on SDS–PAGE. The optimal pH and temperature of the purified enzyme were pH 6.5 and 80°C, respectively. The enzyme showed a broad range of pH stability (pH 4.0–10.5), and was stable up ...
DOI:
10.1016/j.ijbiomac.2014.07.025

5. Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermostable xylanase

Author:
Fan, Guangsen,   et al.   ; Teng, Chao; Yan, Qiaojuan; Jiang, Zhengqiang; Shi, Bo;     Show all 5 Authors
Source:
Bioresource technology 2010 v.101 no.19 pp. 7679-7682
ISSN:
0960-8524
Subject:
Paecilomyces; corn cobs; enzymatic hydrolysis; hydrolysis; industrial applications; pH; steam; syrups; temperature; xylan; xylanases; xylooligosaccharides
Abstract:
... The production of xylooligosaccharides (XOs) from corncobs was studied using a two-stage process based on a steam explosion pretreatment followed by enzymatic hydrolysis. Corncobs with different chip sizes were subjected to steam explosion under different experimental conditions of temperature and time, namely 188-204°C for 2.5-7.5min. The results indicate that corncobs were optimally steam explod ...
DOI:
10.1016/j.biortech.2010.05.004
PubMed:
20554201

6. High-level expression of a xylanase gene from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris

Author:
Fan, Guangsen,   et al.   ; Katrolia, Priti; Jia, Huiyong; Yang, Shaoqing; Yan, Qiaojuan; Jiang, Zhengqiang;     Show all 6 Authors
Source:
Biotechnology letters 2012 v.34 no.11 pp. 2043-2048
ISSN:
0141-5492
Subject:
xylooligosaccharides; Komagataella pastoris; fermenters; xylan; xylanases; Paecilomyces; pH; genes; thermophilic fungi
Abstract:
... A xylanase gene from Paecilomyces thermophila was functionally expressed in Pichia pastoris. The recombinant xylanase (xynA) was predominantly extracellular; in a 5 l fermentor culture, the total extracellular protein was 8.1 g l−1 with an activity of 52,940 U ml−1. The enzyme was purified to homogeneity with a recovery of 48 %. The recombinant xynA was optimally active at 75 °C, as measured over ...
DOI:
10.1007/s10529-012-0995-3
PubMed:
22782269

7. High-level expression of a hyperthermostable Thermotoga maritima xylanase in Pichia pastoris by codon optimization

Author:
Fan, Guangsen,   et al.   ; Jia, Huiyong; Yan, Qiaojuan; Liu, Yuchun; Yan, Ye; Jiang, Zhengqiang;     Show all 6 Authors
Source:
Journal of molecular catalysis 2012 v.78 pp. 72-77
ISSN:
1381-1177
Subject:
sequence analysis; Komagataella pastoris; fermenters; xylanases; nucleotides; genes; nucleotide sequences; pH; Thermotoga maritima; catalytic activity; culture media
Abstract:
... The second xylanase gene (xynB) from the hyperthermophilic Thermotoga maritima was optimized according to the codon usage of Pichia pastoris and expressed in P. pastoris. The optimized gene (xynBop) shared 77.8% of nucleotide sequence identity with that of native gene. A total of 232 nucleotides were changed and the G+C ratio was simultaneously increased from 42.7% to 43.1%. The recombinant xylana ...
DOI:
10.1016/j.molcatb.2012.02.009