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1. A Combination of Phenotypic and Genotypic Characterization Strengthens Pyrenophora tritici-repentis Race Identification

Author:
Andrie, R.M.; Pandelova, I.; Ciuffetti, L.M.
Source:
Phytopathology 2007 v.97 no.6 pp. 694-701
ISSN:
0031-949X
Subject:
races; phenotype; Pyrenophora tritici-repentis; fungal diseases of plants; wheat; pathogen identification; pathogenicity; Triticum aestivum; plant pathogenic fungi; genetic variation; pathotypes
Abstract:
... Pyrenophora tritici-repentis, causal agent of tan spot of wheat, produces multiple host-selective toxins (HSTs), including Ptr ToxA, Ptr ToxB, and Ptr ToxC. The specific complement of HSTs produced by a particular isolate determines its host cultivar specificity. Each unique specificity profile, represented by the differential induction of necrosis or chlorosis on a standard set of wheat different ...
DOI:
10.1094/PHYTO-97-6-0694
PubMed:
18943600

2. A minute virus of canines (MVC: canine bocavirus) isolated from an elderly dog with severe gastroenteritis, and phylogenetic analysis of MVC strains

Author:
Ohshima, T.; Kawakami, K.; Abe, T.; Mochizuki, M.
Source:
Veterinary microbiology 2010 v.145 no.3-4 pp. 334-338
ISSN:
0378-1135
Subject:
gastroenteritis; elderly; strains; Canine minute virus; serodiagnosis; viral diseases of animals and humans; phenotype; dogs; polymerase chain reaction; phylogeny; feces; pathogen identification; pathogenicity; genotype; case studies
Abstract:
... Two of the three adult dogs kept in a family developed severe gastroenteritis. From the feces of one of the affected dogs a minute virus of canines (MVC) was detected by PCR and virus isolation. That this virus had recently infected the dogs was indicated by high anti-MVC antibody titers of their sera. No other virus commonly associated with canine gastrointestinal disease was implicated. As no pr ...
DOI:
10.1016/j.vetmic.2010.03.033
PubMed:
20427134
PubMed Central:
PMC7117362

3. A New Disease, Bacterial Leaf Spot of Rape, Caused by Atypical Pseudomonas viridiflava in South Korea

Author:
Myung, I.S.; Lee, Y.K.; Lee, S.W.; Kim, W.G.; Shim, H.S.; Ra, D.S.
Source:
Plant disease 2010 v.94 no.9 pp. 1164
ISSN:
0191-2917
Subject:
plant pathogenic bacteria; sequence analysis; phenotype; DNA primers; Pseudomonas viridiflava; polymerase chain reaction; bacterial diseases of plants; leaf spot; disease outbreaks; pathogen identification; pathogenicity; nucleotide sequences; genetic variation; Brassica napus var. napus; microbial genetics; rapeseed; disease diagnosis; South Korea
Abstract:
... In March 2007, a bacterial leaf spot of rape (Brassica napus var. oleifera) was observed in fields near Seogwipo City, Jeju Province, South Korea. Symptoms on leaves included white and corky-brown spots and sometimes water-soaked spots on the lower leaf surface. Seven bacterial isolates (BC2495–BC2497 and BC2506–BC2509) were recovered on trypticase soy agar (TSA) from leaf spot lesions surface ste ...
DOI:
10.1094/PDIS-94-9-1164C

4. Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China

Author:
Wang, Dong; Zhang, Limei; Zhou, Xuezhang; He, Yulong; Yong, Changfu; Shen, Mingliang; Szenci, Otto; Han, Bo
Source:
Journal of dairy science 2016 v.99 no.12 pp. 9560-9569
ISSN:
0022-0302
Subject:
Staphylococcus aureus; amoxicillin; animal health; antibiotic resistance; azithromycin; bovine mastitis; clindamycin; cows; dominant genes; farms; genetic variation; genotype; genotyping; herds; microbial detection; milk; pathogen identification; pathogens; phenotype; public health; random amplified polymorphic DNA technique; statistical analysis; sulfamethoxazole; tetracycline; virulence; China
Abstract:
... Staphylococcus aureusis the leading pathogen involved inbovine mastitis, but knowledgeabout antimicrobial resistance, virulence factors, and genotypes of Staphylococcus aureus resulting in bovine mastitis in Ningxia, China, is limited. Therefore, antimicrobial susceptibility, virulence gene, and randomly amplified polymorphic DNA (RAPD) analyses of Staph. aureus were carried out. A total of 327 mi ...
DOI:
10.3168/jds.2016-11625

5. Association of Myroides odoratimimus in immunocompromized piglets with post weaning multisystemic wasting syndrome

Author:
Choudhary, M.; Choudhary, B.K.; Bera, B.C.; Chaudhari, S.P.; Giri, D.K.; Ghosh, R.C.; Barbuddhe, S.B.
Source:
Journal of applied microbiology 2019 v.127 no.6 pp. 1635-1645
ISSN:
1364-5072
Subject:
Myroides; Porcine circovirus-2; aminoglycosides; animal pathogens; antibiotic resistance; carbapenems; cephalosporins; disease severity; fluoroquinolones; health hazards; immunocompromised population; immunosuppression; matrix-assisted laser desorption-ionization mass spectrometry; minimum inhibitory concentration; mortality; multiple drug resistance; pathogen identification; phenotype; phylogeny; piglets; pneumonia; public health; secondary infection; wasting syndrome; weaning
Abstract:
... AIM: To study the association of opportunistic infection due to Myroides odoratimimus in piglets immunocompromised by porcine circovirus type 2 (PCV2) infection. METHODS AND RESULTS: The clinical samples (n = 101) were analysed bacteriologically. The isolates were identified by their phenotypes and MALDI TOF‐MS analysis as Myroides species. The phylogram constructed based on nucleotide sequences o ...
DOI:
10.1111/jam.14448

6. Association of Streptococcus pluranimalium with valvular endocarditis and septicaemia in adult broiler parents

Author:
Hedegaard, L.; Christensen, H.; Chadfield, M.S.; Christensen, J.P.; Bisgaard, M.
Source:
Avian pathology 2009 v.38 no.2 pp. 155-160
ISSN:
0307-9457
Subject:
broiler chickens; disease diagnosis; ribosomal RNA; phenotype; septicemia; poultry diseases; new host records; disease outbreaks; pathogen identification; pathogenicity; mortality; nucleotide sequences; Streptococcus pluranimalium; endocarditis; heart valves; microbial genetics; bacterial infections; disease incidence
Abstract:
... The genus Streptococcus consists of more than 60 species, but only Streptococcus equi subspecies zooepidemicus, Streptococcus gallolyticus ssp. gallolyticus, Streptococcus gallinaceus, Streptococcus dysgalactiae, Streptococcus mutans and Streptococcus suis have been isolated from poultry. During investigations of the aetiology of increased mortality in broiler parent stock at the end of production ...
DOI:
10.1080/03079450902737763
PubMed:
19322715

7. Biological and molecular studies of a cypovirus from the black fly Simulium ubiquitum (Diptera: Simuliidae)

Author:
Green, T.B.; White, S.; Rao, S.; Mertens, P.P.C.; Adler, P.H.; Becnel, J.J.
Source:
Journal of invertebrate pathology 2007 v.95 no.1 pp. 26
ISSN:
0022-2011
Subject:
Simulium; insect viruses; Cypovirus; pathogen identification; viral morphology; genome; double-stranded RNA; phenotype; electrophoresis; molecular systematics; nucleotide sequences; sequence homology; taxonomy
Abstract:
... A cypovirus from the black fly Simulium ubiquitum (SuCPV) was isolated and examined using biological and molecular techniques. SuCPV produces small (typically 0.25 μm), polyhedral shaped inclusion bodies (polyhedra), in which the virus particles become multiply embedded. SuCPV is the third cypovirus isolated from Diptera, but the first from Simuliidae that has been characterized using molecular an ...
Handle:
10113/239
DOI:
10.1016/j.jip.2006.10.006
PubMed:
17224161

8. Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis

Author:
Rajić Savić, Nataša; Katić, Vera; Velebit, Branko
Source:
Acta veterinaria 2014 v.64 no.1 pp. 115-123
ISSN:
1820-7448
Subject:
agar; antibiotic resistance; benzylpenicillin; blood; bovine mastitis; coagulase positive staphylococci; genes; hemolysis; methicillin; milk; minimum inhibitory concentration; oxacillin; pathogen identification; pathogens; phenotype; polymerase chain reaction; virulence
Abstract:
... Coagulase-positive staphylococci are the most common pathogen causing subclinical mastitis in cows. Their main characteristic is a high virulence which leads to chronic infection. A total of 213 isolates of coagulase-positive staphylococci were tested. The majority of isolates (58%) formed a gold pigment, then light gold (28%), white gold (8%), golden gray, creamy white and white (2%). The majorit ...
DOI:
10.2478/acve-2014-0012

9. Characterization and spatial distribution of Aphanomyces in sugarbeet fields

Author:
Dyer, A.T.; Szabo, L.J.; Windels, C.E.
Source:
Journal of sugar beet research 2004 v.41 no.1-2 pp. 1
ISSN:
0899-1502
Subject:
Beta vulgaris; sugar beet; Aphanomyces; root rot; spatial distribution; spatial variation; fields; phenotype; random amplified polymorphic DNA technique; strains; strain differences; pathogenicity; host range; host plants; Aphanomyces cochlioides; pathogen identification; disease diagnosis; fungal anatomy; Minnesota; North Dakota; Texas
Abstract:
... To study phenotypes and spatial distribution of Aphanomyces in sugarbeet fields with histories of root rot, 80 soil cores were collected 1-m apart along a transect in one field each in Minnesota (MN), North Dakota (ND), and Texas (TX). Aphanomyces was isolated from 52, 84, and 49% of the soil cores collected in MN, ND, and TX, respectively. Randomly amplified polymorphic DNA (RAPD) analysis was co ...
Handle:
10113/11550

10. Characterization of Bordetella bronchiseptica strains using phenotypic and genotypic markers

Author:
Friedman, L.E.; Messina, M.T.; Santoferrara, L.; Santillan, M.A.; Mangano, A.; Franco, M.A.
Source:
Veterinary microbiology 2006 v.117 no.2-4 pp. 313-320
ISSN:
0378-1135
Subject:
restriction fragment length polymorphism; host range; phenotype; humans; hosts; strain differences; animal pathogenic bacteria; pathogen identification; genetic variation; Bordetella bronchiseptica; microbial genetics; promoter regions; genetic markers; bacterial toxins
Abstract:
... Thirty-five strains of Bordetella bronchiseptica, recovered primarily from pigs, rabbits, dogs, cats and humans, were characterized by phenotypic and genotypic markers. Biochemical typing only showed variation in the ability to reduce nitrate to nitrite. OMP profiles from virulent strains showed variations in the region of 85-95 kDa, which lead us to describe five OMP-types α, β, γ, δ and epsilon. ...
DOI:
10.1016/j.vetmic.2006.06.005

11. Characterization of Cylindrocarpon Species Associated with Black Foot Disease of Grapevine in Spain

Author:
Alaniz, S.; León, M.; Vicent, A.; García-Jiménez, J.; Abad-Campos, P.; Armengol, J.
Source:
Plant disease 2007 v.91 no.9 pp. 1187-1193
ISSN:
0191-2917
Subject:
Neonectria; grapes; phenotype; sporulation; conidia; fungal diseases of plants; phylogeny; new geographic records; pathogen identification; pathogenicity; genes; nucleotide sequences; microbial growth; Vitis vinifera; microbial genetics; genetic markers; Spain
Abstract:
... In this work, 82 Cylindrocarpon isolates associated with black foot disease of grapevines in Spain were studied by means of phenotypical characterization, DNA analyses, and pathogenicity tests. Partial sequences of the beta-tubulin (BT) gene, BT1, were amplified using primers BT1a and BT1b. A unique and conserved 52-bp insertion in the BT1 sequence, which is a specific marker for C. macrodidymum, ...
DOI:
10.1094/PDIS-91-9-1187

12. Characterization of Pathogenic and Resistant Genome Repertoire Reveals Two Clonal Lines in Salmonella enterica subsp. enterica Serovar Paratyphi B (+)-Tartrate Positive

Author:
Huehn, Stephan; Helmuth, Reiner; Bunge, Cornelia; Guerra, Beatriz; Junker, Ernst; Davies, Rob H.; Wattiau, Pierre; Pelt, Wilfrid van; Malorny, Burkhard
Source:
Foodborne pathogens & disease 2009 v.6 no.4 pp. 431-443
ISSN:
1535-3141
Subject:
bacterial antigens; phenotype; clones; virulence; host range; fimbriae; evolution; serotypes; strain differences; animal pathogenic bacteria; pathogen identification; Salmonella Paratyphi B; microbial genetics; poultry; antibiotic resistance; food pathogens; genetic variation; Germany; Netherlands; Belgium; United Kingdom
Abstract:
... A total of 36 contemporary human, animal, and environmental (+)-tartrate-fermenting (dT+) Salmonella enterica serovar Paratyphi B isolates, formerly called Salmonella serovar Java, and five related monophasic S. enterica serovar 4,5,12:b:- isolates from Belgium, Germany, the Netherlands, and the United Kingdom were investigated for clonality and antimicrobial resistance profiles, as well as their ...
DOI:
10.1089/fpd.2008.0221
PubMed:
19292689

13. Characterization of pathogenic Escherichia coli isolated from humans in Austria: Phenotypes, toxin gene types and epidemiology

Author:
Wagner, M.; Allerberger, F.; Manafi, M.; Lindner, G.; Friedrich, A.W.; Sonntag, A.K.; Foissy, H.
Source:
Zoonoses and public health 2004 v.51 no.6 pp. 288-292
ISSN:
0931-1793
Subject:
human diseases; phenotype; toxigenic strains; virulence; disease transmission; disease outbreaks; gene amplification; pathogen identification; pulsed-field gel electrophoresis; food pathogens; foodborne illness; microbial genetics; bacterial infections; Austria
DOI:
10.1111/j.1439-0450.2004.00776.x
PubMed:
15458492

14. Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France

Author:
Kérouanton, A.; Hennekinne, J.A.; Letertre, C.; Petit, L.; Chesneau, O.; Brisabois, A.; Buyser, M.L. de
Source:
International journal of food microbiology 2007 v.115 no.3 pp. 369-375
ISSN:
0168-1605
Subject:
enterotoxins; bacterial contamination; coagulase negative staphylococci; phenotype; antibiotic resistance; Staphylococcus aureus subsp. aureus; multiple drug resistance; biotypes; pathogen identification; food pathogens; pandemic; staphylococcal intoxication; pulsed-field gel electrophoresis; France
Abstract:
... Enterotoxins produced by Staphylococcus aureus are responsible for staphylococcal food-poisoning outbreaks (SFPO). In France, SFPO are the second cause of food-borne diseases after Salmonella. However, very little is known about the strains involved. The objective of this study was to characterize the staphylococcal strains related to these SFPO through phenotypic and genotypic analyses. A total o ...
DOI:
10.1016/j.ijfoodmicro.2006.10.050

15. Characterization of streptococci and enterococci associated with septicaemia in broiler parents with a high prevalence of endocarditis

Author:
Chadfield, M.S.; Christensen, J.P.; Christensen, H.; Bisgaard, M.
Source:
Avian pathology 2004 v.33 no.6 pp. 610-617
ISSN:
0307-9457
Subject:
broiler breeders; Streptococcus gallinaceus; ribotypes; heart valves; strains; phenotype; spleen; bacteremia; bacterial infections; Enterococcus faecalis; Ross (chicken breed); pathogen identification; disease outbreaks; microbial genetics; mortality; liver; disease prevalence; poultry diseases; nucleotide sequences; endocarditis
DOI:
10.1080/03079450400013089
PubMed:
15763731

16. Characterization, Virulence, Epidemiology, and Management of Anthracnose in Celery

Author:
Rodriguez-Salamanca, Lina M.; Quesada-Ocampo, Lina M.; Naegele, Rachel P.; Hausbeck, Mary K.
Source:
Plant disease 2015 v.99 no.12 pp. 1832-1840
ISSN:
0191-2917
Subject:
Apium graveolens; Colletotrichum acutatum; anthracnose; azoxystrobin; blueberries; celery; chlorothalonil; conidia; crop damage; dew; disease incidence; environmental factors; field experimentation; genotype; greenhouse experimentation; growing season; growth chambers; leaf curling; leaf wetness; mancozeb; microsatellite repeats; pathogen identification; pathogens; petioles; phenotype; pyraclostrobin; temperature; tomatoes; virulence; Michigan
Abstract:
... Leaf curling and petiole twisting of celery (Apium graveolens) were observed in several commercial fields in five Michigan counties in 2010 through 2012, causing significant crop damage and loss. Prior to this time, the pathogen Colletotrichum acutatum species complex had not been previously associated with celery in Michigan. In this study, the pathogen’s genotype and phenotype were characterized ...
DOI:
10.1094/PDIS-09-14-0994-RE

17. Coagulase-negative staphylococci isolated from bovine extramammary sites and intramammary infections in a single dairy herd

Author:
Taponen, Suvi; Bjorkroth, Johanna; Pyorala, Satu
Source:
Journal of dairy research 2008 v.75 no.4 pp. 422-429
ISSN:
0022-0299
Subject:
dairy cows; perineum; restriction fragment length polymorphism; diagnostic techniques; DNA libraries; ribosomal RNA; coagulase negative staphylococci; food handling; hands; phenotype; animal pathogenic bacteria; lactation; disease transmission; pathogen identification; teats; pulsed-field gel electrophoresis; bovine mastitis; animal handling; udders; disease diagnosis
Abstract:
... Isolates of various species of coagulase-negative staphylococci (CNS) from extramammary swab samples were compared with isolates of bovine mastitis CNS species. Swab samples were taken from perineum skin and udder skin, teat apices and teat canals of lactating dairy cows of the research dairy herd of the University of Helsinki in 1999 and 2002. In addition, hands of herd staff and liners of teat c ...
DOI:
10.1017/S0022029908003312
PubMed:
18700996

18. Comparison of a commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequence-based genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis

Author:
Capurro, A.; Artursson, K.; Waller, K. Persson; Bengtsson, B.; Ericsson-Unnerstad, H.; Aspán, A.
Source:
Veterinary microbiology 2009 v.134 no.3-4 pp. 327-333
ISSN:
0378-1135
Subject:
ribosomal RNA; validity; genes; drug resistance; interspecific variation; phenotype; genotype; disease detection; cattle; pathogen identification; pathogenicity; molecular epidemiology; microbial genetics; accuracy; disease diagnosis; coagulase negative staphylococci; nucleotide sequences; anti-infective agents; bovine mastitis
Abstract:
... In order to evaluate the usefulness of some phenotypic and genotypic methods for species identification of coagulase-negative staphylococci (CNS), isolates were obtained from bovine cases of clinical and sub-clinical mastitis from different geographical areas in Sweden. By using the Staph-Zym[trade mark sign] test, antimicrobial susceptibility testing, and sequencing of part of the CNS tuf gene an ...
DOI:
10.1016/j.vetmic.2008.08.028
PubMed:
18930604

19. Diversity and Biogeography of Sooty Blotch and Flyspeck Fungi on Apple in the Eastern and Midwestern United States

Author:
Díaz Arias, Maria M.; Batzer, Jean C.; Harrington, Thomas C.; Wong, Amy Wang; Bost, Steven C.; Cooley, Daniel R.; Ellis, Michael A.; Hartman, John R.; Rosenberger, David A.; Sundin, George W.; Sutton, Turner B.; Travis, James W.; Wheeler, Michael J.; Yoder, Keith S.; Gleason, Mark L.
Source:
Phytopathology 2010 v.100 no.4 pp. 345-355
ISSN:
0031-949X
Subject:
Malus domestica; apples; plant pathogenic fungi; fungal diseases of plants; signs and symptoms (plants); pathogen identification; disease diagnosis; disease incidence; geographical distribution; phenotype; internal transcribed spacers; genetic markers; ribosomal DNA; pathogenicity; fungicides; United States
Abstract:
... Sooty blotch and flyspeck (SBFS) fungi on apple fruit were sampled from nine orchards in four midwestern U.S. states during 2000 and 30 orchards in 10 eastern U.S. states during 2005 in order to estimate taxonomic diversity and discern patterns of geographic distribution. Forty apple fruit per orchard were arbitrarily sampled and colonies of each mycelial phenotype were counted on each apple. Repr ...
DOI:
10.1094/PHYTO-100-4-0345
PubMed:
20205538

20. Diversity of free-living amoebae in soils and their associated human opportunistic bacteria

Author:
Denet, Elodie; Coupat-Goutaland, Bénédicte; Nazaret, Sylvie; Pélandakis, Michel; Favre-Bonté, Sabine
Source:
Parasitology research 2017 v.116 no.11 pp. 3151-3162
ISSN:
0932-0113
Subject:
Acanthamoeba; Actinobacteria; Bacteroidetes; Burkholderia cepacia; Dictyostelium discoideum; Escherichia coli; Firmicutes; Naegleria; Pseudomonas aeruginosa; Stenotrophomonas maltophilia; agricultural soils; antibiotic resistance; bacteria; gardens; horticultural soils; humans; mining; models; pathogen identification; pathogens; phagocytosis; phenotype; polymerase chain reaction; ribosomal DNA; secondary infection; sequence analysis; virulence; Alps region; Burkina Faso; France; Vietnam
Abstract:
... Free-living amoebae (FLA) are ubiquitous protozoa found worldwide in the environment. They feed by phagocytosis on various microorganisms. However, some bacteria, i.e., amoebae-resistant bacteria (ARB) or bacterial endocytobionts, can resist phagocytosis and even multiply inside FLA. This study investigated the diversity of culturable FLA in various soils from agricultural and mining sites and the ...
DOI:
10.1007/s00436-017-5632-6

21. DNA barcoding of fungi causing infections in humans and animals

Author:
Irinyi, Laszlo; Lackner, Michaela; de Hoog, G. Sybren; Meyer, Wieland
Source:
Fungal biology 2016 v.120 no.2 pp. 125-136
ISSN:
1878-6146
Subject:
DNA barcoding; animal pathogenic fungi; barcoding; databases; diagnostic techniques; humans; internal transcribed spacers; mycology; pathogen identification; phenotype; ribosomal DNA; species identification
Abstract:
... Correct species identification is becoming increasingly important in clinical diagnostics. Till now, many mycological laboratories rely on conventional phenotypic identification. But this is slow and strongly operator-dependent. Therefore, to improve the quality of pathogen identification, rapid, reliable, and objective identification methods are essential. One of the most encouraging approaches i ...
DOI:
10.1016/j.funbio.2015.04.007

22. Drippy Pod of White Lupine: A New Bacterial Disease Caused by a Pathovar of Brenneria quercina

Author:
Lu, Shi-En; Gross, Dennis C.
Source:
Plant disease 2010 v.94 no.12 pp. 1431-1440
ISSN:
0191-2917
Subject:
plant pathogenic bacteria; sequence analysis; disease diagnosis; fatty acids; phenotype; signs and symptoms (plants); ribosomal DNA; host plants; new taxa; strain differences; bacterial diseases of plants; pathogen identification; pathogenicity; nucleotide sequences; legumes; pathotypes; Lupinus albus; microbial genetics; Brenneria quercina
Abstract:
... Drippy pod is a unique bacterial disease of Mediterranean white lupine (Lupinus albus) that first appeared in commercial fields in Eastern Washington State in the mid-1980s. The disease is most noticeable in the field as water-soaked lesions on lupine pods that produce an abundance of whitish-colored ooze with a sticky and foamy consistency. As the disease progresses, yellowing of lupine plants oc ...
DOI:
10.1094/PDIS-05-10-0365

23. Employment of 16S rDNA gene sequencing techniques to identify phenotypically difficult-to-identify culturable eubacteria from foods and waters

Author:
Xi, J.; Heaney, J.C.N.; Marshal, S.A.; Millar, B.C.; McDowell, D.A.; McMahon, A.; Blair, I.S.; Rooney, P.J.; Moore, J.E.
Source:
International journal of food science & technology 2005 v.40 no.2 pp. 229-233
ISSN:
0950-5423
Subject:
Eubacteria; food contamination; bacterial contamination; sequence analysis; nucleotide sequences; pathogen identification; water pollution; ribosomal DNA; genes; phenotype; polymerase chain reaction; reliability; public health; human diseases; disease diagnosis; Northern Ireland
DOI:
10.1111/j.1365-2621.2004.00936.x

24. Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

Author:
Vera Lizarazo, Y.A.; Rodríguez Ferri, E.F.; Gutiérrez Martín, C.B.
Source:
Research in veterinary science 2008 v.85 no.3 pp. 453-456
ISSN:
0034-5288
Subject:
disease detection; disease diagnosis; bacterial pneumonia; phenotype; swine diseases; validity; pathogen identification; pneumonic pasteurellosis; pathotypes; strains; accuracy; Pasteurella multocida; swine
Abstract:
... An exhaustive biochemical characterisation of 60 porcine Pasteurella multocida clinical isolates recovered from lesions indicative of pneumonia, previously confirmed by PCR and all belonging to the capsular serogroup A, was performed by means of four commercial systems. The API 20NE correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the API 20E method ...
DOI:
10.1016/j.rvsc.2008.02.002
PubMed:
18343468

25. Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees

Author:
Alippi, A.M.; Lopez, A.C.; Reynaldi, F.J.; Grasso, D.H.; Aguilar, O.M.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 290-303
ISSN:
0378-1135
Subject:
epidemiological studies; tetracycline; phenotype; antibiotic resistance; Paenibacillus larvae; minimum inhibitory concentration; geographical variation; honey bees; plasmids; American foul brood; pathogen identification; genes; molecular epidemiology; reference standards; genetic variation; oxytetracycline; virulence; bacterial infections; defense mechanisms
Abstract:
... Paenibacillus larvae is the causal agent of American Foulbrood (AFB) disease, the most virulent bacterial disease of honeybee (Apis mellifera L.) brood. Oxytetracycline is the main antibiotic used for prevention and control of AFB. Using the polymerase chain reaction, isolates were screened for the presence of the tetracycline resistance tet(K) and tet(L) determinants. Four isolates (5%), which co ...
DOI:
10.1016/j.vetmic.2007.05.018

26. First case of Edwardsiella ictaluri infection in China farmed yellow catfish Pelteobagrus fulvidraco

Author:
Ye, Shigen; Li, Hua; Qiao, Guo; Li, Zhongshi
Source:
Aquaculture 2009 v.292 no.1-2 pp. 6-10
ISSN:
0044-8486
Subject:
fish farms; ribosomal RNA; Bagridae; freshwater fish; farmed fish; Edwardsiella ictaluri; phenotype; freshwater aquaculture; bacteremia; new geographic records; Gram-negative bacteria; sequence analysis; disease detection; animal pathogenic bacteria; disease outbreaks; pathogen identification; pathogenicity; mortality; fish culture; bacterial enteritis; fish diseases; catfish; signs and symptoms (animals and humans); China
Abstract:
... The first epizootic of yellow catfish Pelteobagrus fulvidraco (10g-250g) caused by Edwardsiella ictaluri, is described. Bacteria were isolated from yellow catfish, P. fulvidraco, kept in a pond in China. During the outbreak, the cumulative mortality was up to 50%. Acute disease was characterized grossly by cutaneous haemorrhage, ulceration and enteritis, while chronic disease a soft, fluctuant red ...
DOI:
10.1016/j.aquaculture.2009.03.036

27. First Report of Anthracnose of Camellia sasanqua Caused by Colletotrichum aenigma in China

Author:
Chen, X.; Wang, T.; Guo, H.; Zhu, P. K.; Xu, L.
Source:
Plant disease 2019 v.103 no.6 pp. 1423
ISSN:
0191-2917
Subject:
Camellia sasanqua; Colletotrichum; DNA; Glomerella cingulata; anthracnose; appressoria; chitin synthase; color; conidia; defoliation; disease incidence; flowers; foliar diseases; fungi; glyceraldehyde-3-phosphate dehydrogenase; grapes; greenhouses; landscaping; leaf spot; leaves; mycelium; ornamental plants; pathogen identification; pathogenicity; phenotype; relative humidity; strawberries; streptomycin; summer; tissues; tree diseases; trees; China
Abstract:
... Camellia sasanqua belongs to the family Theaceae and possesses oval-shaped leaves and plum-shaped flowers. This ornamental plant has been widely used in landscaping owing to its graceful form and bright-colored flowers and leaves. In the summer of 2015 and 2016, a serious leaf spot disease was detected on trees of C. sasanqua (78% of 50 randomly selected trees) planted on the campus of East China ...
DOI:
10.1094/PDIS-11-18-1971-PDN

28. First Report of Rosa multiflora cryptic virus in Rosa multiflora in the Eastern United States

Author:
Martin, R.R.; Tzanetakis, I.E.
Source:
Plant disease 2008 v.92 no.12 pp. 1706
ISSN:
0191-2917
Subject:
Rosa multiflora; ornamental woody plants; nursery crops; Partitiviridae; plant diseases and disorders; new geographic records; pathogen identification; double-stranded RNA; disease diagnosis; signs and symptoms (plants); polymerase chain reaction; nucleotide sequences; amino acid sequences; phenotype; mixed infection; Arkansas
DOI:
10.1094/PDIS-92-12-1706B

29. First Report of Soybean dwarf virus in Soybean in Northern Illinois

Author:
Thekkeveetil, T.; Hobbs, H.A.; Wang, Y.; Kridelbaugh, D.; Donnelly, J.; Hartman, G.L.; Domier, L.L.
Source:
Plant disease 2007 v.91 no.12 pp. 1686
ISSN:
0191-2917
Subject:
Glycine max; soybeans; Soybean dwarf virus; plant diseases and disorders; new geographic records; disease surveillance; pathogen identification; host plants; host range; Trifolium; signs and symptoms (plants); reverse transcriptase polymerase chain reaction; enzyme-linked immunosorbent assay; disease incidence; phenotype; microbial genetics; amino acid sequences; nucleotide sequences; Illinois
DOI:
10.1094/PDIS-91-12-1686B

30. First Report of Xanthomonas citri pv. citri-A* Causing Citrus Canker on Lime in Cambodia

Author:
Bui Thi Ngoc, L.; Vernière, C.; Pruvost, O.; So, T.; Johnson, G.I.
Source:
Plant disease 2008 v.92 no.11 pp. 1588
ISSN:
0191-2917
Subject:
plant pathogenic bacteria; limes; amplified fragment length polymorphism; phenotype; selective media; disease severity; cankers (plants); pathotypes; new geographic records; Xanthomonas citri; host range; Citrus aurantiifolia; strain differences; bacterial diseases of plants; pathogen identification; pathogenicity; disease diagnosis; nucleotide sequences; Cambodia
Abstract:
... Asiatic citrus canker caused by Xanthomonas citri pv. citri (X. citri pv. citri-A) is detrimental to citrus production in tropical and subtropical areas. The bacterium can cause severe infection on many citrus species, initially causing water-soaked leaf lesions that become erumpent and necrotic, often with a chlorotic halo. Severe infection causes premature fruit drop and twig dieback. X. citri p ...
DOI:
10.1094/PDIS-92-11-1588A

31. First Report on the Presence of Phoma Blackleg Pathogenicity Group 1 (Leptosphaeria biglobosa) on Brassica napus (Canola/Rapeseed) in Iran

Author:
Fernando, W.G.D.; Ghanbarnia, K.; Salati, M.
Source:
Plant disease 2007 v.91 no.4 pp. 465
ISSN:
0191-2917
Subject:
disease diagnosis; phenotype; relative humidity; fungal diseases of plants; strain differences; disease outbreaks; pathogen identification; plant pathogenic fungi; new crops; Leptosphaeria; Brassica napus; virulence; new geographic records; disease incidence; Iran
DOI:
10.1094/PDIS-91-4-0465A

32. Further data on the production of beauvericin, enniatins and fusaproliferin and toxicity to Artemia salina by Fusarium species of Gibberella fujikuroi species complex

Author:
Moretti, A.; Mule, G.; Ritieni, A.; Logrieco, A.
Source:
International journal of food microbiology 2007 v.118 no.2 pp. 158-163
ISSN:
0168-1605
Subject:
rice; phenotype; biomarkers; Oryza sativa; Fusarium fujikuroi; Artemia salina; bioassays; pathogen identification; toxicity testing; plant pathogenic fungi; fumonisins; animal models; shrimp; toxicity; pathotypes; food safety; metabolites
Abstract:
... The knowledge of toxigenic profiles of fungal plant pathogens is of extreme importance for evaluating the potential toxicity of infected plant products. Ninety-six fungal isolates belonging to 28 species in the Gibberella fujikuroi complex were studied for the production of beauvericin, enniatins and fusaproliferin in rice cultures. Toxin production ranged from 5 to 3000 μg/g for beauvericin, 2 to ...
DOI:
10.1016/j.ijfoodmicro.2007.07.004

33. Genetic diversity and antimicrobial susceptibility profiles among mastitis-causing Staphylococcus aureus isolated from bovine milk samples

Author:
Anderson, K.L.; Lyman, R.L.; Bodeis-Jones, S.M.; White, D.G.
Source:
American journal of veterinary research 2006 v.67 no.7 pp. 1185-1191
ISSN:
0002-9645
Subject:
dairy cows; Staphylococcus aureus; phenotype; antibiotic resistance; strain differences; animal pathogenic bacteria; pathogen identification; milk; genotype; genetic variation; bovine mastitis; disease diagnosis; North Carolina; Virginia
DOI:
10.2460/ajvr.67.7.1185
PubMed:
16817741

34. Genetic variation of single nucleotide polymorphisms identified at the mating type locus correlates with form-specific disease phenotype in the barley net blotch fungus Pyrenophora teres.

Author:
Lu, Shunwen; Edwards, Michael C.; Friesen, Timothy L.
Source:
European journal of plant pathology 2013 v.135 pp. 49
ISSN:
0929-1873
Subject:
DNA primers; Pyrenophora teres; barley; fungal diseases of plants; fungi; genetic variation; loci; nucleotide sequences; pathogen identification; phenotype; phenotypic variation; phylogeny; polymerase chain reaction; single nucleotide polymorphism; strains; virulence; Australia; California
Abstract:
... Mating-type (MAT) locus-specific single nucleotide polymorphisms (SNPs) have been shown to be sufficient for conventional PCR-based differentiation of Pyrenophora teres f. teres (Ptt) and P. teres f. maculata (Ptm), the cause of the net and spot form, respectively, of barley net blotch (Lu et al. 2010, Phytopathology, 100:1298-1306). Here, we report the cloning and characterization of the MAT locu ...
Handle:
10113/58156
DOI:
10.1007/s10658-012-0064-8

35. Genotyping and phylogenetic analysis of bovine viral diarrhea virus isolates from BVDV infected alpacas in North America

Author:
Kim, Sung G.; Anderson, Renee R.; Yu, Jin Z.; Zylich, Nancy C.; Kinde, Hailu; Carman, Suzanne; Bedenice, Daniela; Dubovi, Edward J.
Source:
Veterinary microbiology 2009 v.136 no.3-4 pp. 209-216
ISSN:
0378-1135
Subject:
genome; phylogeny; alpacas; strains; phenotype; bovine viral diarrhea; pathotypes; epidemiological studies; disease detection; pathogen identification; chronic diseases; molecular epidemiology; disease surveillance; alternative livestock; disease prevalence; reverse transcriptase polymerase chain reaction; longitudinal studies; Bovine viral diarrhea virus 1; nucleotide sequences; North America
Abstract:
... Over a three-year period, 2004-2007, greater than 12,000 alpacas in the United States were screened by real-time RT-PCR to identify alpacas persistently infected (PI) with bovine viral diarrhea virus (BVDV). A total of 46 BVD viruses were isolated from PI alpacas or diagnostic samples from alpacas. Forty-three US alpaca BVDV isolates and 3 Canadian isolates were analyzed by comparison of nucleotid ...
DOI:
10.1016/j.vetmic.2008.10.029
PubMed:
19059738

36. High diversity of bacterial pathogens and antibiotic resistance in salmonid fish farm pond water as determined by molecular identification employing 16S rDNA PCR, gene sequencing and total antibiotic susceptibility techniques

Author:
Moore, John E.; Huang, Junhua; Yu, Pengbo; Ma, Chaofeng; Moore, Peter JA; Millar, Beverley C.; Goldsmith, Colin E.; Xu, Jiru
Source:
Ecotoxicology and environmental safety 2014 v.108 pp. 281-286
ISSN:
0147-6513
Subject:
Acinetobacter; Aeromonas; Chryseobacterium; Erwinia; Flavobacterium; Pseudomonas; Rheinheimera; Salmonidae; antibiotic resistance; ciprofloxacin; disease outbreaks; fish; fish diseases; fish farms; pathogen identification; pathogens; phenotype; plate count; polymerase chain reaction; ribosomal DNA; sequence analysis; tetracycline; Northern Ireland
Abstract:
... The aim of this study was to examine the microbiological and related parameters (antibiotic resistance and pathogen identification) of water at two salmonid fish farms in Northern Ireland. Total Bacterial Counts at the Movanagher Fish Farm was 1730 colony forming units (cfu)/ml water (log10 3.24cfu/ml) and 3260cfu/ml (log10 3.51cfu/ml) at the Bushmills Salmon Station. Examination of resulting orga ...
DOI:
10.1016/j.ecoenv.2014.05.022

37. Host Specialization not Detected Among Isolates of the EC-1 Lineage of Phytophthora infestans Attacking Wild and Cultivated Potatoes in Peru

Author:
Garry, G.; Salas, A.; Forbes, G.A.; Perez, W.; Santa Cruz, M.; Nelson, R.J.
Source:
European journal of plant pathology 2005 v.113 no.1 pp. 71-81
ISSN:
0929-1873
Subject:
mitochondrial DNA; Solanum tuberosum; fungicide resistance; potatoes; metalaxyl; phenotype; haplotypes; allozymes; wild relatives; virulence; restriction fragment length polymorphism; host range; cultivars; strain differences; Phytophthora infestans; pathogen identification; host specificity; microbial genetics; genetic variation; Peru
Abstract:
... To determine whether populations of Phytophthora infestans attacking wild and cultivated potatoes in the highlands of Peru are specialized on their hosts of origin, we characterized isolates using several neutral markers, metalaxyl resistance and for aggressiveness in a detached leaf assay. One hundred and fifty-three isolates were collected from the northern and central highlands of Peru from dif ...

38. Identification, mycotoxin risk and pathogenicity of Fusarium species associated with fig endosepsis in Apulia, Italy

Author:
Moretti, A.; Ferracane, L.; Somma, S.; Ricci, V.; Mule, G.; Susca, A.; Ritieni, A.; Logrieco, A.F.
Source:
Food additives & contaminants 2010 v.27 no.5 pp. 718-728
Subject:
Ficus carica; plant pathogenic fungi; mass spectrometry; figs; fumonisin B2; capillary gas chromatography; phenotype; postharvest diseases; fungal diseases of plants; secondary metabolites; genotype; virulence; Fusarium solani; sequence analysis; fumonisin B1; quantitative analysis; Fusarium proliferatum; pathogen identification; in vitro studies; molds (fungi); food contamination; food pathogens; fungal spores; Italy
Abstract:
... In a survey carried out on 87 rotted fig fruits samples collected in the Apulia region of Italy, the authors isolated 126 Fusarium strains identified as F. ramigenum (69 strains), F. solani (49), F. proliferatum (five) and three not identified. Investigation on the fertility of the strains belonging to F. proliferatum and F. ramigenum revealed that only strains of F. proliferatum were fertile. The ...
DOI:
10.1080/19440040903573040
PubMed:
20352549

39. Molecular Epidemiology of Microorganisms Isolated from Food Workers and Enteral Feeding of Public Hospitals

Author:
Borges, Liana J.; Campos, Maria Raquel H.; Cardoso, Juliana L.; André, Maria Cláudia D.P.B.; Serafini, Álvaro B.
Source:
Journal of food science 2010 v.75 no.7 pp. M449
ISSN:
0022-1147
Subject:
liquid diet; Staphylococcus aureus; food service workers; bacterial contamination; Escherichia coli; phenotype; food handling; hospitals; food contamination; animal pathogenic bacteria; disease transmission; pathogen identification; food pathogens; pulsed-field gel electrophoresis; strains; enteral feeding; Brazil
Abstract:
... This study aimed to compare strains of Staphylococcus aureus and E. coli isolated from food workers and enteral diet samples obtained from 2 public hospitals (H1/H2) in Goiania, Goias, Brazil, by the means of antibiogram and pulsed field gel electrophoresis (PFGE). In the H1, strains of S. aureus were present in 2 enteral diet samples and in 13 food worker swabs. Strains of E. coli were found in a ...
DOI:
10.1111/j.1750-3841.2010.01751.x
PubMed:
21535555

40. Multiplex polymerase chain reaction for identification of Escherichia coli, Escherichia albertii and Escherichia fergusonii

Author:
Lindsey, Rebecca L.; Garcia-Toledo, L.; Fasulo, D.; Gladney, L.M.; Strockbine, N.
Source:
Journal of microbiological methods 2017
ISSN:
0167-7012
Subject:
Escherichia albertii; Escherichia coli; Escherichia fergusonii; Shigella; bacteria; biosynthesis; computer software; cysteine; pathogen identification; phenotype; polymerase chain reaction; regulator genes; transactivators
Abstract:
... Escherichia coli, Escherichia albertii, and Escherichia fergusonii are closely related bacteria that can cause illness in humans, such as bacteremia, urinary tract infections and diarrhea. Current identification strategies for these three species vary in complexity and typically rely on the use of multiple phenotypic and genetic tests. To facilitate their rapid identification, we developed a multi ...
DOI:
10.1016/j.mimet.2017.06.005
PubMed:
28599915
PubMed Central:
PMC5603207

41. Multisystemic Abscesses in African Green Monkeys (Chlorocebus aethiops) with Invasive Klebsiella pneumoniae--Identification of the Hypermucoviscosity Phenotype

Author:
Twenhafel, N.A.; Whitehouse, C.A.; Stevens, E.L.; Hottel, H.E.; Foster, C.D.; Gamble, S.; Abbott, S.; Janda, J.M.; Kreiselmeier, N.; Steele, K.E.
Source:
Veterinary pathology 2008 v.45 no.2 pp. 226-231
ISSN:
0300-9858
Subject:
Cercopithecus aethiops; monkeys; Klebsiella pneumoniae; bacterial infections; histopathology; abscess; pathogenesis; immunohistochemistry; pathogen identification; phenotype; serotypes
Abstract:
... Invasive Klebsiella pneumoniae is an emerging disease of humans characterized by abscesses in the liver or other sites involving bacteria with the unique hypermucoviscosity phenotype. Over several months, 7 African green monkeys in our research colony developed abscess formation in multiple locations and succumbed to disease. K. pneumoniae was identified by bacterial culture in 6 monkeys and immun ...
DOI:
10.1354/vp.45-2-226
PubMed:
18424839

42. Occurrence and Characterization of a Phytophthora sp. Pathogenic to Asparagus (Asparagus officinalis) in Michigan

Author:
Saude, C.; Hurtado-Gonzales, O.P.; Lamour, K.H.; Hausbeck, M.K.
Source:
Phytopathology 2008 v.98 no.10 pp. 1075-1083
ISSN:
0031-949X
Subject:
metalaxyl; Asparagus officinalis; amplified fragment length polymorphism; phenotype; plant rots; pathogen identification; pathogenicity; plant pathogenic fungi; genotype; genetic variation; Phytophthora megasperma; Michigan
Abstract:
... A homothallic Phytophthora sp. was recovered from asparagus (Asparagus officinalis) spears, storage roots, crowns, and stems in northwest and central Michigan in 2004 and 2005. Isolates (n = 131) produced ovoid, nonpapillate, noncaducous sporangia 45 μm long x 26 μm wide and amphigynous oospores of 25 to 30 μm diameter. Mycelial growth was optimum at 25°C with no growth at 5 and 30°C. All isolates ...
DOI:
10.1094/PHYTO-98-10-1075
PubMed:
18943453

43. Occurrence of Race 3 of Phytophthora nicotianae in North Carolina, the Causal Agent of Black Shank of Tobacco

Author:
Gallup, C.A.; Shew, H.D.
Source:
Plant disease 2010 v.94 no.5 pp. 557-562
ISSN:
0191-2917
Subject:
Nicotiana tabacum; tobacco; drug crops; Phytophthora nicotianae; plant pathogenic fungi; fungal diseases of plants; new geographic records; pathotypes; cultivars; disease resistance; genetic resistance; genes; virulence; pathogen identification; host plants; phenotype; seedling diseases; North Carolina
Abstract:
... Black shank, caused by the oomycete Phytophthora nicotianae, causes significant annual yield losses in tobacco. Race 3 of P. nicotianae is reported here for the first time from North Carolina. It was identified from a North Carolina tobacco field with a history of tobacco varieties with Phl gene resistance and numerous field sites with no known deployment of varieties with the Phl gene. Race 3 was ...
DOI:
10.1094/PDIS-94-5-0557

44. PAMDB, A Multilocus Sequence Typing and Analysis Database and Website for Plant-Associated Microbes

Author:
Almeida, Nalvo F.; Yan, Shuangchun; Cai, Rongman; Clarke, Christopher R.; Morris, Cindy E.; Schaad, Norman W.; Schuenzel, Erin L.; Lacy, George H.; Sun, Xiaoan; Jones, Jeffrey B.; Castillo, Jose A.; Bull, Carolee T.; Leman, Scotland; Guttman, David S.; Setubal, João C.; Vinatzer, Boris A.
Source:
Phytopathology 2010 v.100 no.3 pp. 208
ISSN:
0031-949X
Subject:
plant pathogenic bacteria; bacteria; phenotype; pathogen identification; nucleotide sequences; microbial genetics; genetic variation; databases; information dissemination; evolution; population genetics; microbial ecology; epidemiology; Internet; phylogeny; signs and symptoms (plants); Acidovorax avenae subsp. citrulli; Pseudomonas syringae; Ralstonia solanacearum; Xanthomonas
Abstract:
... Although there are adequate DNA sequence differences among plant-associated and plant-pathogenic bacteria to facilitate molecular approaches for their identification, identification at a taxonomic level that is predictive of their phenotype is a challenge. The problem is the absence of a taxonomy that describes genetic variation at a biologically relevant resolution and of a database containing re ...
Handle:
10113/41307
DOI:
10.1094/PHYTO-100-3-0208
PubMed:
20128693

45. Performance of API Staph ID 32 and Staph-Zym for identification of coagulase-negative staphylococci isolated from bovine milk samples

Author:
Sampimon, O.C.; Zadoks, R.N.; De Vliegher, S.; Supré, K.; Haesebrouck, F.; Barkema, H.W.; Sol, J.; Lam, T.J.G.M.
Source:
Veterinary microbiology 2009 v.136 no.3-4 pp. 300-305
ISSN:
0378-1135
Subject:
dairy cows; cattle diseases; sequence analysis; disease detection; sampling; coagulase negative staphylococci; phenotype; validity; pathogen identification; food pathogens; milk; analytical kits; molecular epidemiology; screening; accuracy; bacterial infections; prediction; disease diagnosis
Abstract:
... In this study, the accuracy of two phenotypic tests, API Staph ID 32 and Staph-Zym, was determined for identification of coagulase-negative staphylococci (CNS) from bovine milk samples in comparison with identification based on DNA-sequencing. A total of 172 CNS isolated from bovine milk were classified into 17 species. The most frequently isolated species based on rpoB sequencing were Staphylococ ...
DOI:
10.1016/j.vetmic.2008.11.004
PubMed:
19110383

46. Pheno-genotypic characterisation of Escherichia coli O157:H7 isolates from domestic and wild ruminants

Author:
Sánchez, S.; Martínez, R.; Rey, J.; García, A.; Blanco, J.; Blanco, M.; Blanco, J.E.; Mora, A.; Herrera-León, S.; Echeita, A.; Alonso, J.M.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 445-449
ISSN:
0378-1135
Subject:
Shiga-like toxins; pulsed-field gel electrophoresis; phenotype; feces; genotype; wild animals; cattle; polymerase chain reaction; strain differences; animal pathogenic bacteria; pathogen identification; microbial genetics; Escherichia infections; disease reservoirs; toxigenic strains; sheep; disease transmission; Escherichia coli O157; goat milk; deer; Spain
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) O157:H7 represents a major public health concern worldwide, with ruminants recognised as their main natural reservoir. The aim of this work was to determine the phenotypic features and genetic relationships of 46 E. coli O157:H7 isolates obtained from sheep, cattle and deer faeces and from unpasteurised goat milk in Spain over a period of 11 years. Cha ...
DOI:
10.1016/j.vetmic.2009.10.009
PubMed:
19914011

47. Phenotypic and Etiological Differences Between Psyllid Yellows and Zebra Chip Diseases of Potato

Author:
Sengoda, Venkatesan G.; Munyaneza, Joseph E.; Crosslin, James M.; Buchman, Jeremy L.; Pappu, Hanu R.
Source:
American journal of potato research 2010 v.87 no.1 pp. 41
ISSN:
1099-209X
Subject:
Solanum tuberosum; potatoes; Candidatus Liberibacter; bacterial diseases of plants; plant diseases and disorders; Psyllidae; insect pests; insect vectors; plant pests; phenotype; signs and symptoms (plants); etiology; aerial parts; tubers; disease course; polymerase chain reaction; pathogen identification; disease detection; leaves; mortality; Bactericera cockerelli
Abstract:
... Both potato psyllid yellows and zebra chip (ZC) potato diseases are associated with the potato psyllid, Bactericera cockerelli (Sulc). Aboveground plant symptoms of both diseases are similar but there is a difference in symptoms in potato tubers. ZC has recently been associated with a new species of the bacterium liberibacter, ‘Candidatus Liberibacter solanacearum’, also known as ‘Ca. Liberibacter ...
Handle:
10113/41624
DOI:
10.1007/s12230-009-9115-x

48. Phenotypic and Genetic Analysis of Epiphytic Pseudomonas syringae Populations from Sweet Cherry in Michigan

Author:
Renick, L.J.; Cogal, A.G.; Sundin, G.W.
Source:
Plant disease 2008 v.92 no.3 pp. 372-378
ISSN:
0191-2917
Subject:
plant pathogenic bacteria; phenotype; cankers (plants); biological resistance; copper; polymerase chain reaction; strain differences; frost injury; bacterial diseases of plants; pathogen identification; pathogenicity; microbial genetics; Prunus avium; ice nucleation; epiphytes; flowers; Pseudomonas syringae pv. syringae; Pseudomonas syringae pv. morsprunorum; genetic variation; cherries; Michigan
Abstract:
... A severe outbreak of bacterial canker occurred on sweet cherry in Michigan in 2002. Blossom infection and subsequent canker formation was observed following a prolonged freeze event during bloom. Epiphytic blossom isolates of Pseudomonas syringae were recovered from 39 orchards from the three major cherry-growing areas (southwest [SW], west-central [WC], and northwest [NW]) of Michigan in 2003 and ...
DOI:
10.1094/PDIS-92-3-0372

49. Phenotypic and Genetic Characterization of Pasteurella Multocida and Related Isolates from Rabbits in Switzerland

Author:
Stahel, Anina B. J.; Hoop, Richard K.; Kuhnert, Peter; Korczak, Bożena M.
ISSN:
1040-6387
Subject:
rabbits; disease diagnosis; ribosomal RNA; phenotype; polymerase chain reaction; rabbit diseases; phylogeny; Pasteurella multocida subsp. multocida; pathogen identification; nucleotide sequences; pathotypes; strains; DNA-directed RNA polymerase; Pasteurella canis; Pasteurella multocida subsp. septica; pasteurellosis; Switzerland
Abstract:
... Several bacteria belonging to the family Pasteurellaceae are potential pathogens in rabbits. In particular, Pasteurella multocida is considered to be important, and outbreaks caused by this species result in considerable economic losses in rabbitries. However, Pasteurellaceae spp. isolated from rabbits are poorly characterized, and thus, proper identification of P. multocida isolates from these an ...
DOI:
10.1177/104063870902100605
PubMed:
19901279

50. Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia

Author:
Bankole, A.A.; Saegerman, C.; Berkvens, D.; Fretin, D.; Geerts, S.; Ieven, G.; Walravens, K.
Source:
The Veterinary record 2010 v.166 no.24 pp. 753-756
ISSN:
0042-4900
Subject:
phenotype; blood serum; loci; cattle; orchitis; strain differences; tandem repeat sequences; abortion (animals); enzyme-linked immunosorbent assay; pathogen identification; genotype; bovine brucellosis; Brucella melitensis biovar Abortus; disease diagnosis; Gambia
DOI:
10.1136/vr.b4862

51. Phenotypic and genotypic characteristics of methicillin/oxacillin-resistant Staphylococcus intermedius isolated from clinical specimens during routine veterinary microbiological examinations

Author:
El Zubeir, I.E.M.; Kanbar, T.; Alber, J.; Lammler, C.; Akineden, O.; Weiss, R.; Zschock, M.
Source:
Veterinary microbiology 2007 v.121 no.1-2 pp. 170-176
ISSN:
0378-1135
Subject:
Staphylococcus aureus; Staphylococcus intermedius; phenotype; antibiotic resistance; oxacillin; strain differences; pathogen identification; genotype; microbial detection; clinical examination; veterinary clinics; methicillin; Germany
Abstract:
... Methicillin/oxacillin resistance of 10 S. intermedius strains was investigated by conventional and molecular methods. The strains tested had been isolated in Germany during routine veterinary microbiological examinations of specimens from a small animal clinic between May and September 2005. Epidemiological relationships of the strains were studied by macrorestriction analysis of their chromosomal ...
DOI:
10.1016/j.vetmic.2006.11.014

52. Phenotypic and genotypic characterization of antimicrobial resistance in faecal enterococci from wild boars (Sus scrofa)

Author:
Poeta, P.; Costa, D.; Igrejas, G.; Rodrigues, J.; Torres, C.
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 368-374
ISSN:
0378-1135
Subject:
enteropathogens; strains; drug resistance; phenotype; swine diseases; feces; risk assessment; genotype; bacterial infections; defense mechanisms; epidemiological studies; Enterococcus; wildlife diseases; Sus scrofa; pathogen identification; wild boars; microbial genetics; disease reservoirs; disease prevalence; disease transmission; digestive system diseases; anti-infective agents; Portugal
Abstract:
... The objective was to study the prevalence of antimicrobial resistance and the mechanisms implicated in faecal enterococci of wild boars in Portugal. One hundred and thirty-four enterococci (67 E. faecium, 54 E. hirae, 2 E. faecalis, 2 E. durans and 9 Enterococcus spp.) were recovered from 67 wild boars (two isolates/sample), and were further analysed. High percentages of resistance were detected f ...
DOI:
10.1016/j.vetmic.2007.06.003

53. Phenotypic and genotypic characterization of Paenibacillus larvae isolates

Author:
Antunez, K.; Piccini, C.; Castro-Sowinski, S.; Rosado, A.S.; Seldin, L.; Zunino, P.
Source:
Veterinary microbiology 2007 v.124 no.1-2 pp. 178-183
ISSN:
0378-1135
Subject:
American foul brood; bacterial proteins; DNA fingerprinting; strains; gel electrophoresis; phenotype; genotype; bacterial infections; epidemiological studies; geographical distribution; Apis mellifera; polymerase chain reaction; honey bees; pathogen identification; molecular epidemiology; antibiotic resistance; Paenibacillus larvae; disease prevalence; genetic variation; Argentina; Uruguay
Abstract:
... Paenibacillus larvae is the causative agent of American Foulbrood (AFB), a severe disease of honeybees (Apis melifera). The aim of this work was to develop a strategy for the subtyping and the epidemiological analysis of P. larvae. Phenotypic characterisation, susceptibility to several antibiotics, electrophoresis of whole bacterial proteins, rep-PCR, ribotyping and DGGE were assessed using a coll ...
DOI:
10.1016/j.vetmic.2007.04.012

54. Phenotypic and genotypic characterization of Prototheca zopfii in a dog with enteric signs

Author:
Ribeiro, Marcio Garcia; Farias, Marconi Rodrigues de; Roesler, Uwe; Roth, Katja; Rodigheri, Sabrina Mari; Ostrowsky, Marco Antonio; Salerno, Tatiana; Siqueira, Amanda Keller; Fernandes, Marta Catarina
Source:
Research in veterinary science 2009 v.87 no.3 pp. 479-481
ISSN:
0034-5288
Subject:
drug therapy; phenotype; dog diseases; dogs; bioassays; pathogen identification; chronic diseases; rectum; scanning electron microscopy; Prototheca; enteritis; genotype; case studies; cell biology; signs and symptoms (animals and humans); disease diagnosis; Brazil
Abstract:
... This is a case report of enteric protothecosis caused by Prototheca zopfii in an eight-year-old male mixed breed dog with a history of chronic bloody diarrhea, loss of appetite and weight loss. Algae were isolated from rectal scrapings in defibrinated sheep blood agar and dextrose Sabouraud agar. Cytological evaluation showed the presence of globular and cylindrical organisms with a defined capsul ...
DOI:
10.1016/j.rvsc.2009.04.015
PubMed:
19520405

55. Phenotypic and genotypic characterization of Staphylococcus aureus isolated from raw camel milk samples

Author:
Shuiep, E.S.; Kanbar, T.; Eissa, N.; Alber, J.; Lammler, C.; Zschock, M.; El Zubeir, I.E.M.; Weiss, R.
Source:
Research in veterinary science 2009 v.86 no.2 pp. 211-215
ISSN:
0034-5288
Subject:
Staphylococcus aureus; raw milk; camel milk; animal diseases; phenotype; dairy animals; risk assessment; dairy farming; pathotypes; bacterial infections; epidemiological studies; enterotoxins; strain differences; pathogen identification; pathogenicity; molecular epidemiology; food safety; disease surveillance; camels; disease prevalence; food pathogens; foodborne illness; Sudan
Abstract:
... In the present study 320 milk samples collected from 160 apparently healthy camels of three different locations in Sudan were investigated for the presence of Staphylococcus aureus resulting in the isolation of this bacterial pathogen from 28 milk samples from 24 camels. Twenty-five S. aureus were identified phenotypically and by PCR mediated amplification of species-specific genes or gene segment ...
DOI:
10.1016/j.rvsc.2008.07.011
PubMed:
18778841

56. Phenotypic and molecular characterization of Brachyspira spp. isolated from laying hens in different housing systems

Author:
Jansson, D.S.; Fellström, C.; Råsbäck, T.; Vågsholm, I.; Gunnarsson, A.; Ingermaa, F.; Johansson, K.-E.
Source:
Veterinary microbiology 2008 v.130 no.3-4 pp. 348-362
ISSN:
0378-1135
Subject:
epidemiological studies; Brachyspira; ribosomal RNA; phenotype; laying hens; avirulent strains; disease prevalence; poultry housing; polymerase chain reaction; poultry diseases; pathogen identification; molecular epidemiology; digestive system diseases; genetic variation; pathotypes; bacterial infections
Abstract:
... Several species of intestinal spirochaetes, Brachyspira (B.) alvinipulli, B. intermedia and B. pilosicoli, may cause reduced egg production and faecal staining of eggshells in chickens. The aim of this study was to characterize potentially pathogenic and presumably non-pathogenic Brachyspira spp. from commercial laying hens. Selective culture, phenotyping, PCR and 16S rRNA gene sequencing were use ...
DOI:
10.1016/j.vetmic.2008.02.010
PubMed:
18375077

57. Phenotypic and Molecular Typing of Tuberculous and Nontuberculous Mycobacterium Species from Slaughtered Pigs in Egypt

Author:
Mohamed, Amr M.; El-Ella, Ghada A. Abou; Nasr, Essam A.
ISSN:
1040-6387
Subject:
interspecific variation; phenotype; meat inspection; polymerase chain reaction; strain differences; animal pathogenic bacteria; Mycobacterium bovis; Rhodococcus equi; pathogen identification; nucleotide sequences; disease incidence; genetic variation; Mycobacterium tuberculosis; microbial genetics; swine; Egypt
Abstract:
... A total of 745 slaughtered pigs were examined during routine meat inspection for suspected tuberculous lesions. Specimens from suspected lesions were collected for conventional mycobacteriologic examinations. Suspected mycobacterial colonies were subjected to molecular typing based on the Mycobacterium species-specific intergenic spacer (IGS) target. The study resulted in detection of suspected le ...
DOI:
10.1177/104063870902100107
PubMed:
19139500

58. Phenotypic characterization of mastitic Prototheca spp. isolates

Author:
Marques, Sara; Silva, Eliane; Carvalheira, Júlio; Thompson, Gertrude
Source:
Research in veterinary science 2010 v.89 no.1 pp. 5-9
ISSN:
0034-5288
Subject:
phenotype; dairy cattle; pathogen identification; biochemistry; milk; Prototheca; computer software; bovine mastitis; disease diagnosis
Abstract:
... Bovine mastitis associated with Prototheca is considered a rare pathology, but is increasing in prevalence all over the world and therefore becoming more relevant to the dairy industry. The biochemical characterization of 47 Prototheca isolates retrieved from mastitic milk was performed in this study using API 20C Aux and two BBL Crystal Kits, followed by an analysis with InforBio software. The us ...
DOI:
10.1016/j.rvsc.2009.12.017
PubMed:
20096906

59. Phylogenetic analysis of SRLV sequences from an arthritic sheep outbreak demonstrates the introduction of CAEV-like viruses among Spanish sheep

Author:
Glaria, I.; Reina, R.; Crespo, H.; de Andrés, X.; Ramírez, H.; Biescas, E.; Pérez, M.M.; Badiola, J.; Luján, L.; Amorena, B.; de Andrés, D.
Source:
Veterinary microbiology 2009 v.138 no.1-2 pp. 156-162
ISSN:
0378-1135
Subject:
genome; arthritis; phylogeny; sheep diseases; strains; phenotype; viral diseases of animals and humans; Caprine arthritis encephalitis virus; pathotypes; epidemiological studies; sequence analysis; amino acid sequences; disease outbreaks; pathogen identification; molecular epidemiology; microbial genetics; sheep; emerging diseases; genomics; nucleotide sequences; genetic variation; Spain
Abstract:
... Small ruminant lentiviruses (SRLVs) cause different clinical forms of disease in sheep and goats. So far in Spain, Maedi visna virus-like (MVV-like) sequences have been found in both species, and the arthritic SRLV disease has never been found in sheep until a recent outbreak. Knowing that arthritis is common in goats, it was of interest to determine if the genetic type of the virus involved in th ...
DOI:
10.1016/j.vetmic.2009.03.002
PubMed:
19339126

60. Phytophthora Database: A Forensic Database Supporting the Identification and Monitoring of Phytophthora

Author:
Park, Jongsun; Park, Bongsoo; Veeraraghavan, Narayanan; Jung, Kyongyong; Lee, Yong-Hwan; Rossman, Amy; Farr, David; Cline, Erica; Grünwald, Niklaus J.; Luster, Douglas G.; Schrandt, Julia; Martin, Frank
Source:
Plant disease 2008 v.92 no.6 pp. 966
ISSN:
0191-2917
Subject:
Phytophthora; plant pathogenic fungi; disease surveillance; pathogen identification; databases; plant pathology; epidemiology; microbial ecology; genotype; phenotype; strains; pathotypes; risk assessment; world wide web; phylogeny; nucleotide sequences
Abstract:
... Phytophthora spp. represent a serious threat to agricultural and ecological systems. Many novel Phytophthora spp. have been reported in recent years, which is indicative of our limited understanding of the ecology and diversity of Phytophthora spp. in nature. Systematic cataloging of genotypic and phenotypic information on isolates of previously described species serves as a baseline for identific ...
Datasets:
  Phytophthora Database
Handle:
10113/21199
DOI:
10.1094/PDIS-92-6-0966

61. Preliminary molecular identification of drug resistant cyathostomes in Italy

Author:
Lia, R. P.; Traversa, D.; Iorio, R.; Otranto, D.; Klei, T. R.; Ricci, V.; Giangaspero, A.
Source:
Veterinary research communications 2008 v.32 pp. 211-213
ISSN:
0165-7380
Subject:
benzimidazole; animal parasites and pests; ivermectin; phenotype; pyrantel; nematode infections; strain differences; multiple drug resistance; fenbendazole; moxidectin; pathogen identification; fecal egg count; nucleic acid hybridization; horses; Cyathostominae; Italy
DOI:
10.1007/s11259-008-9156-5

62. Production of a d-glycero-d-manno-heptosyltransferase mutant of Mannheimia haemolytica displaying a veterinary pathogen specific conserved LPS structure; development and functionality of antibodies to this LPS structure

Author:
Logan, S.M.; Chen, W.; Aubry, A.; Gidney, M.A.J.; Lacelle, S.; St. Michael, F.; Kuolee, R.; Higgins, M.; Neufeld, S.; Cox, A.D.
Source:
Veterinary microbiology 2006 v.116 no.1-3 pp. 175-186
ISSN:
0378-1135
Subject:
pathogen identification; mutants; Mannheimia haemolytica; lipopolysaccharides; antibody formation; gene expression regulation; monoclonal antibodies; mutagenesis; phenotype; gene transfer; in vitro studies
Abstract:
... Previous structural studies of the lipopolysaccharides from the veterinary pathogens Mannheimia haemolytica (Mh), Actinobacillus pleuropneumoniae (Ap) and Pasteurella multocida (Pm) had identified a conserved inner core oligosaccharide structure that was present in all strains investigated. In order to examine the potential of this inner core structure as a vaccine, a mutagenesis strategy was adop ...
DOI:
10.1016/j.vetmic.2006.04.024

63. Pulsed-field gel electrophoresis patterns and antimicrobial susceptibility phenotypes for coagulase-positive staphylococcal isolates from pustules and carriage sites in dogs with superficial bacterial folliculitis

Author:
Pinchbeck, L.R.; Cole, L.K.; Hillier, A.; Kowalski, J.J.; Rajala-Schultz, P.J.; Bannerman, T.L.; York, S.
Source:
American journal of veterinary research 2007 v.68 no.5 pp. 535-542
ISSN:
0002-9645
Subject:
Staphylococcus; phenotype; antibiotic resistance; catalase; dog diseases; antibiotics; dogs; multiple drug resistance; strain differences; animal pathogenic bacteria; pathogen identification; hair follicles; pulsed-field gel electrophoresis; genotype; bacterial infections
DOI:
10.2460/ajvr.68.5.535
PubMed:
17472455

64. Routine Phenotypic Identification of Bacterial Species of the Family Pasteurellaceae Isolated from Animals

Author:
Dousse, Florence; Thomann, Andreas; Brodard, Isabelle; Korczak, Bozena M.; Schlatter, Yvonne; Kuhnert, Peter; Miserez, Raymond; Frey, Joachim
ISSN:
1040-6387
Subject:
NAD (coenzyme); taxonomy; phenotype; Actinobacillus; Mannheimia; respiratory tract diseases; Haemophilus; phylogeny; wildlife diseases; pathogen identification; rapid methods; validity; molecular genetics; genes; hemolysis; molecular epidemiology; bacterial infections; disease diagnosis
Abstract:
... Pasteurellaceae are bacteria with an important role as primary or opportunistic, mainly respiratory, pathogens in domestic and wild animals. Some species of Pasteurellaceae cause severe diseases with high economic losses in commercial animal husbandry and are of great diagnostic concern. Because of new data on the phylogeny of Pasteurellaceae, their taxonomy has recently been revised profoundly, t ...
DOI:
10.1177/104063870802000602
PubMed:
18987220

65. Sources of Resistance to Stem Rust Race Ug99 in Spring Wheat Germplasm

Author:
Rouse, M.N.; Wanyera, R.; Njau, P.; Jin, Y.
Source:
Plant disease 2011 v.95 no.6 pp. 762
ISSN:
0191-2917
Subject:
Puccinia graminis; Triticum aestivum; breeding; breeding lines; cultivars; disease resistance; evolutionary adaptation; genes; germplasm; pathogen identification; pathogens; phenotype; plant breeders; plant nurseries; races; screening; seasons; seedlings; spring wheat; stem rust; virulence; Iran; Kenya; Minnesota; Uganda; Yemen
Abstract:
... Wheat stem rust (Puccinia graminis f. sp. tritici) race TTKSK (Ug99), with virulence to the majority of the world's wheat (Triticum aestivum) cultivars, has spread from Uganda throughout eastern Africa, Yemen, and Iran. The identification and spread of variants of race TTKSK with virulence to additional stem rust resistance genes has reminded breeders and pathologists of the danger of deploying ma ...
Handle:
10113/59195
DOI:
10.1094/PDIS-12-10-0940

66. Soybean Sudden Death Syndrome Species Diversity Within North and South America Revealed by Multilocus Genotyping

Author:
O'Donnell, Kerry; Sink, Stacy; Scandiani, María Mercedes; Luque, Alicia; Colletto, Analía; Biasoli, Marisa; Lenzi, Lisandro; Salas, Graciela; González, Victoria; Ploper, Leonardo Daniel; Formento, Norma; Pioli, Rosanna N.; Aoki, Takayuki; Yang, X.B.; Sarver, Brice A.J.
Source:
Phytopathology 2010 v.100 no.1 pp. 58
ISSN:
0031-949X
Subject:
Glycine max; soybeans; field crops; Fusarium solani; Fusarium solani f. sp. phaseoli; plant pathogenic fungi; fungal diseases of plants; pathogen identification; disease diagnosis; pathogenicity; phenotype; microbial genetics; phylogeny; diagnostic techniques; nucleotide sequences; sequence analysis; accuracy; genotype; genetic polymorphism; ribosomal DNA; loci; flow cytometry
Abstract:
... Sudden death syndrome (SDS) of soybean has become a serious constraint to the production of this crop in North and South America. Phenotypic and multilocus molecular phylogenetic analyses, as well as pathogenicity experiments, have demonstrated that four morphologically and phylogenetically distinct fusaria can induce soybean SDS. Published molecular diagnostic assays for the detection and identif ...
Handle:
10113/38775
DOI:
10.1094/PHYTO-100-1-0058
PubMed:
19968550

67. Species identification of coagulase-negative staphylococci: Genotyping is superior to phenotyping

Author:
Zadoks, Ruth N.; Watts, Jeffrey L.
Source:
Veterinary microbiology 2009 v.134 no.1-2 pp. 20-28
ISSN:
0378-1135
Subject:
dairy cows; sequence analysis; heifers; interspecific variation; coagulase negative staphylococci; phenotype; host-pathogen relationships; accuracy; validity; pathogen identification; pathogenicity; genotype; molecular epidemiology; genetic variation; bovine mastitis; disease diagnosis
Abstract:
... Coagulase-negative staphylococci (CNS) are isolated commonly from bovine milk and skin. Their impact on udder health and milk quality is debated. It has been suggested that sources and consequences of infection may differ between CNS species. Species-specific knowledge of the impact and epidemiology of CNS intramammary infections is necessary to evaluate whether species-specific infection control ...
DOI:
10.1016/j.vetmic.2008.09.012

68. Spread of levan-positive populations of Pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot, in central Italy

Author:
Marchi, G.; Viti, C.; Giovannetti, L.; Surico, G.
Source:
European journal of plant pathology 2005 v.112 no.2 pp. 101-112
ISSN:
0929-1873
Subject:
Pseudomonas syringae pv. savastanoi; plant pathogenic bacteria; bacterial diseases of plants; Olea europaea; olives; levan; pathogen identification; phylogeny; disease transmission; orchards; phenotype; genetic variation; pathogenicity; Italy

69. Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance

Author:
Dubska, Lenka; Pilatova, Katerina; Dolejska, Monika; Bortlicek, Zbynek; Frostova, Tereza; Literak, Ivan; Valik, Dalibor
Source:
Anaerobe 2011 v.17 no.6 pp. 444-447
ISSN:
1075-9964
Subject:
Escherichia coli; antibiotic resistance; antibiotics; bacterial proteins; desorption; diagnostic techniques; mass spectrometry; microbiology; pathogen identification; phenotype; rapid methods
Abstract:
... Based on experiments with 10 defined strains of Escherichia coli, we present a new method for bacterial phenotyping using SELDI-TOF mass spectrometry. Changes in bacterial protein profiles in the context of the time of cultivation and the antibiotic environment were minimal. Proteom subprofiling may further distinguish between strains with specific susceptibility to antimicrobials. Mass spec-based ...
DOI:
10.1016/j.anaerobe.2011.05.008

70. Susceptibility of Fraser Fir to Phytophthora capsici

Author:
Quesada-Ocampo, L.M.; Fulbright, D.W.; Hausbeck, M.K.
Source:
Plant disease 2009 v.93 no.2 pp. 135-141
ISSN:
0191-2917
Subject:
Abies fraseri; Christmas trees; forest plantations; forest trees; Phytophthora capsici; plant pathogenic fungi; fungal diseases of plants; seedlings; disease resistance; plant damage; root systems; tree diseases; tree trunk; signs and symptoms (plants); mortality; pathogen identification; phenotype; soil-borne diseases; soil fungi; Michigan
Abstract:
... Phytophthora cinnamomi, P. drechsleri, P. citricola, and P. cactorum limit Fraser fir production, whereas P. capsici affects Solanaceous, Cucurbitaceous, and Fabaceous crops. Some vegetable growers in Michigan plant conifers for the Christmas tree market in fields infested with P. capsici. To determine the susceptibility of Fraser fir to P. capsici, stems (no wound or 1- or 3-mm-diameter wound) or ...
DOI:
10.1094/PDIS-93-2-0135

71. The quest for the perfect test: Phenotypic versus genotypic identification of coagulase-negative staphylococci associated with bovine mastitis

Author:
Ruegg, Pamela L.
Source:
Veterinary microbiology 2009 v.134 no.1-2 pp. 15-19
ISSN:
0378-1135
Subject:
dairy cows; heifers; disease detection; milk analysis; coagulase negative staphylococci; phenotype; accuracy; validity; disease control; pathogen identification; genotype; molecular epidemiology; analytical kits; bovine mastitis; disease diagnosis
Abstract:
... Coagulase-negative staphylococci (CNS) are a frequent cause of bovine intramammary infection and the objective of this paper is to discuss the utility of phenotypic identification systems used for species identification of CNS relative to use of genotypic identification. Mastitis control programs have been developed for major mastitis pathogens but few are specifically targeted for control of CNS. ...
DOI:
10.1016/j.vetmic.2008.09.013
PubMed:
19022591

72. Thiolated AuNP probes and multiplex PCR for molecular detection of Staphylococcus epidermidis

Author:
Osmani Bojd, Mahsa; Kamaladini, Hossein; Haddadi, Fatemeh; Vaseghi, Akbar
Source:
Molecular and cellular probes 2017 v.34 pp. 30-36
ISSN:
0890-8508
Subject:
Acinetobacter baumannii; DNA; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; absorption; coagulase negative staphylococci; color; colorimetry; essential genes; nanogold; pathogen identification; phenotype; polymerase chain reaction
Abstract:
... The emergence of nanotechnology in biology helps to apply the gold nanoparticle probes for fast and accurate identification of pathogens compared to the time-consuming and non-precise phenotypic methods. In this study, two molecular methods have been established for the accurate identification of staphylococcus epidermidis from other coagulase-negative staphylococci. Multiplex PCR was performed us ...
DOI:
10.1016/j.mcp.2017.04.006

73. Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle

Author:
Evans, Nicholas J.; Brown, Jennifer M.; Demirkan, Ibrahim; Murray, Richard D.; Vink, W. Daan; Blowey, Roger W.; Hart, C. Anthony; Carter, Stuart D.
Source:
Veterinary microbiology 2008 v.130 no.1-2 pp. 141-150
ISSN:
0378-1135
Subject:
cattle diseases; sequence analysis; ribosomal RNA; phenotype; enzyme activity; phylogeny; dairy cattle; pathogen identification; molecular epidemiology; genes; Treponema medium; dermatitis; Treponema denticola; bacterial infections; Treponema phagedenis; feet; United Kingdom
Abstract:
... Bovine digital dermatitis (BDD) is a severe infectious cause of lameness which has spread through dairy cattle populations worldwide, causing serious welfare and agricultural problems. Spirochetes are the main organisms implicated and have previously proven difficult to isolate. This study aimed to isolate and characterise the range of spirochetes associated with BDD in the UK. Twenty-three spiroc ...
DOI:
10.1016/j.vetmic.2007.12.019
PubMed:
18243592

74. Variation of Lipopolysaccharide among the Three Major Agrobacterium Species and the Effect of Environmental Stress on the Lipopolysaccharide Profile

Author:
Arafat, H.H.; Tanaka, K.; Sawada, H.; Suzuki, K.
Source:
Plant pathology journal 2009 v.8 no.1 pp. 1-8
ISSN:
1812-5425
Subject:
interspecific variation; acidity; lipopolysaccharides; phenotype; molecular weight; Agrobacterium radiobacter; strain differences; ambient temperature; Rhizobium rhizogenes; heat stress; alkalinity; cold stress; pathogen identification; biochemical pathways; phenotypic variation; Rhizobium vitis; salt stress
Abstract:
... Lipopplysaccharide (LPS) is a variable component among the bacterial species as wall as strains of a single species and this characteristic is helpful for discrimination between strains. However, we have only limited information about LPS variation and influence by environment in Agrobacterium strains. In this study, we analyzed variation of lipopolysaccharide (LPS) among 34 Agrobacterium strains; ...
DOI:
10.3923/ppj.2009.1.8

75. Xanthomonas species causing bacterial spot of tomato in the Russian federation

Author:
Kornev, K.P.; Matveeva, E.V.; Pekhtereva, E.Sh.; Polityko, V.A.; Ignatov, A.N.; Punina, N.V.; Schaad, N.W.
Source:
Acta horticulturae 2009 no.808 pp. 243
ISSN:
0567-7572
Subject:
Solanum lycopersicum var. lycopersicum; tomatoes; bacterial wilt; Xanthomonas; Xanthomonas vesicatoria; Xanthomonas gardneri; Xanthomonas campestris pv. vesicatoria; plant pathogenic bacteria; pathogenicity; signs and symptoms (plants); leaf spotting; disease detection; pathogen identification; polymerase chain reaction; gene amplification; DNA fingerprinting; nucleotide sequences; pathotypes; genotype; phenotype; amylose; pectins; biodegradation; strain differences; Russia
Abstract:
... Bacterial spot of tomato, caused by Xanthomonas euvesicatoria (Group A), X. vesicatoria (Group B), X. perforans (group C) and X. gardneri (Group D) (Jones et al., 2004), formerly known as X. campestris pv. vesicatoria, has become very important in the Russian Federation. Leaf spots and wilt symptoms were observed in 2006 in tomato fields located in southern European part of Russia. Field symptoms ...
Handle:
10113/31982