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Mohamed N. Helaly; Hanan M. El-Hoseiny; Nabil I. Elsheery; Hazem M. Kalaji; Sergio de los Santos-Villalobos; Jacek Wróbel; Islam F. Hassan; Maybelle S. Gaballah; Lamyaa A. Abdelrhman; Amany M. Mira; Shamel M. Alam-Eldein
... Plant growth, development, and productivity are adversely affected under drought conditions. Previous findings indicated that 5-aminolevulinic acid (ALA) and 24-epibrassinolide (EBL) play an important role in the plant response to adverse environmental conditions. This study demonstrated the role of ALA and EBL on oxidative stress and photosynthetic capacity of drought-stressed ‘Williams’ banana g ...
... OBJECTIVES: To investigate the level of 5-aminolevulinic acid (5-ALA), a key precursor of heme, and expression of heme-peroxidase on the regulation of heme synthesis in E. coli. METHODS: A transporter gene (eamA) was knocked out, and glutamyl-tRNA reductase gene (hemA) for 5-ALA synthesis and a dye-decolorizing peroxidase gene (DyP) were overexpressed. RESULTS: Knockout of eamA caused decrease of ...
... Cytochrome P450 monooxygenases (P450s) are promising versatile oxidative biocatalysts. However, the practical use of P450s in vitro is limited by their dependence on the co-enzyme NAD(P)H and the complex electron transport system. Using H₂O₂ simplifies the catalytic cycle of P450s; however, most P450s are inactive in the presence of H₂O₂. By mimicking the molecular structure and catalytic mechanis ...
... Controlling the size of nanocrystals and inhibiting their agglomeration are of paramount importance for achieving ideal catalytic performance. Here we discovered that carbon dots (CDs) are not only able to serve as reductants but also as stabilizers of ultrasmall Co₉S₈ nanocrystals by means of their surface terminal groups. As a result, ultrasmall Co₉S₈ nanocrystals are incorporated into porous ca ...
... We developed a rapid and sensitive colorimetric biosensor based on competitive recognition between kanamycin (KAN), magnetic beads-kanamycin (MBs-KAN) and aptamer and terminal deoxynucleotidyl transferase (TdT)-mediated signal amplification strategy. In the absence of KAN, aptamers recognize MBs-KAN. TdT can amplify oligonucleotides to the 3′-OH ends of aptamers, with biotin-dUTP being embedded in ...
... It is challenging to capture glycoprotein from complicated samples with both high specificity and high recovery. Herein, we synthesized temperature-responsive poly (N-isopropylacrylamide-4-vinylbenzeneboronic acid) (PNIPAM-VPBA) microgels having enhanced affinity capability towards glycoprotein, resulting signal amplification in colorimetric analysis subsequently. Through temperature control, the ...
... Passion fruit woodiness disease is responsible for severe losses in passion fruit production around the world. The disease is caused by Cowpea aphid-borne mosaic virus (CABMV), an aphid-transmitted potyvirus. Traditional sanitary measures against the disease, such as vector elimination and cross protection, have not been successful, resulting in elimination and replanting of passion fruit plants e ...
... The control of pesticide residues in food is of increasing importance nowadays due to the over-use and misapplication of herbicides in agricultural production. However, the current colorimetric method for rapid detection of glyphosate still faces many challenges like the low sensitivity and stability. Herein, a simple and ultrasensitive liquid colorimetric chemosensor for glyphosate detection was ...
... The occurrence of contaminants such as heavy metals in an aqueous environment has become a global concern. In the present study, a bio-filter was designed to eliminate heavy metals from river wastewater contaminated with industrial effluents. Moreover, we analyzed simple tap water, bio-filtered water, and unfiltered river wastewater and measured the concentrations of different heavy metals in the ...
... Artificial peroxisome is of critical importance to supersede natural peroxisome in fabricating protocell system and disease treatment. Nevertheless, developing feasible artificial peroxisome with various stable functions remains a monumental challenge. Nanozyme with multiple enzyme-like activities can mimic natural enzymes in peroxisome, which make it a prospective candidate for artificial peroxis ...
... A self-cleaning electrochemical biosensor based on two-dimensional Cu-porphyrin (Cu-TCPP) metal-organic framework nanofilms, novel super G-quadruplex (G4), and DNA nanomotors was developed for the cyclic detection of Pb²⁺ ions. The Cu-TCPP framework with inherent peroxidase activity can create an ultra-thin nanofilm that functioned as a carrier to support the metastable G4 comprising four individu ...
... Broad bean mottle virus (BBMV) infects a wide range of hosts, resulting in significant production reductions. The lack of adequate and effective control methods involves implementing novel BBMV control strategies. Herein, we demonstrate the effect of different potassium concentrations (20, 40, and 60 mM) against BBMV in broad bean plants. Potassium could control BBMV infection in broad bean by inh ...
2,4-dichlorophenol; cathodes; electric power; liquid chromatography; mass spectrometry; oxidation; peroxidase; photocatalysis; pollutants; pollution control; solar radiation; wastewater treatment
Abstract:
... Photoelectrocatalytic (PEC) technology has been considered as one of the most efficient advanced oxidation processes for wastewater treatment, but the necessity of external electric power supply limits the portability of PEC system for on-field applications. Herein, a portable solar-powered biophotoelectrocatalytic system driven by photovoltaic (PV) cells was explored for degradation of pollutant ...
... The combination of bio- and chemo-catalysts for sequential cascades has received considerable attention in analytical fields because of the regulable catalytic efficiency and selectivity under various physiological conditions. In this paper, a versatile multienzyme cascade nanoplatform with excellent activity for biosensing is demonstrated by combining metal–organic framework (MOF)-based nanozyme ...
... Current study investigated the effects of potassium metabisulfite (E224), a common food preservative, in male rats following 28 days of oral exposure at doses: 0.7, 7.0, and 70 mg/kg b.w. Following autopsies, blood and tissues samples (liver, kidney, brain, and testes) were taken. Data were compared statistically at p < .05. Potassium metabisulfite caused significant dose‐dependent reduction in mo ...
aldehydes; blood serum; chemical species; fluorescence; glucose; humans; pH; peroxidase
Abstract:
... Numerous assays based on peroxidase activity have been developed for the detection of analytes due to the various optical peroxidase substrates. However, most substrates are sensitive to light and pH and are over-oxidized in the presence of excess H₂O₂. In this study, 2-((6-methoxynaphthalen-2-yl)methylene)-1,1-dimethylhydrazine (MNDH), a fluorescent peroxidase substrate prepared from naphthalene- ...
... Cadmium (Cd) is a hazardous metal that has a significant risk of transfer from soil to edible parts of food crops including shoots and seeds. Reduction of Cd accumulation is required to lower the risk of Cd exposure in humans and animals feeding on metal contaminated parts of such plants. Coriandrum sativum L. (coriander) exposed to Cd showed stress symptoms such as stunted growth, reduced photosy ...
... Beneficial interaction of plants with fungi is one strategy for adapting to environmental stresses like drought. Serendipita indica, an endophytic root fungus, promotes plant growth and enhances tolerance to abiotic stresses. The present study aimed to investigate the amelioration of drought stress in tomato (Solanum lycopersicum L.) upon S. indica and glucose application through morpho-physiologi ...
... Fruit freckles of ‘Namwa’ banana (Musa x paradisiaca) are a disorder observed as small brown spots or freckles on the fruit surface that become scabby during the preharvest and postharvest stages. To investigate the cause of disorder, morphology and molecular properties of isolated fungi as well as the biochemical properties of infected bananas were examined. Scanning electron microscopy revealed ...
... Peanut [Arachis hypogea (L.)] is a valuable legume crop that is widely cultivated in Northern Nigeria. The yield of this crop is affected by root rot disease caused by the fungal pathogen (Macrophomina phaseolina). This study was carried out to assess the efficacy of Zinc (Zn) (1.76, 3.59, 4.71 and 6 mg kg⁻¹) and Manure [M (2% and 4%)] in a complex interaction to manage the disease and improve yie ...
Arabidopsis; Malus domestica; apples; aquaporins; arginine; electrolyte leakage; genes; growth and development; malondialdehyde; peroxidase; plant response; salt stress; salt tolerance; secondary metabolites; stress tolerance; superoxide dismutase; transcriptomics
Abstract:
... Aquaporins are known as water channel proteins. In this study, an aquaporin gene MdPIP1;2 was cloned from Malus domestica cv. Qinguan encoding a protein of 289 amino acids that formed the typical structure of aquaporin by six transmembrane domains, two asparagine–proline–alanine motifs, aromatic/arginine filter, and Forger's position. MdPIP1;2 was highly expressed in the water-sensitive or water-r ...
Chinese cabbage; acephate; acetylcholine; acetylcholinesterase; chlorides; choline oxidase; color; colorimetry; coprecipitation; detection limit; food chemistry; glucose; peroxidase; porous media
Abstract:
... An efficient colorimetric detection platform based on multi-enzyme cascade has been developed for detection of organophosphorus. Firstly, the dual-enzyme platform was prepared and applied for sensitive glucose detection (detection limit 0.32 μM). And then three enzymes, including acetylcholinesterase, horseradish peroxidase and choline oxidase were encapsulated in cruciate flower-like zeolitic imi ...
... This work evaluates the thermal inactivation of peroxidase and polyphenol oxidase of açai-berry pulp in a continuous pasteurization using a lab-scale pasteurizer with plate heat exchangers (PHEs). Experiments were carried-out at three different açai flow rates with two different pass arrangements of the PHEs. The experimental treatments were simulated by CFD and the numerical results were compared ...
... This work presented an electrochemical biosensor for the detection of virulence outer membrane protein A (ompA) gene of Cronobacter sakazakii (C. sakazakii), which was based on the mimic peroxidase activity of boron doped quantum dots-Au nanoparticles (BQDs-AuNPs) and a signal amplification strategy of exonuclease III (Exo III)-assisted target-recycling (EATR). The electrochemical signal was come ...
... Premature leaf senescence has a profound influence on crop yield and quality. Here, a stable premature senescence mutant (GSm) was obtained from the common wheat (Triticum aestivum L.) cultivar Chang 6878 by mutagenesis with ethyl methanesulfonate. The differences between the GSm mutant and its wild-type (WT) were analyzed in terms of yield characteristics, photosynthetic fluorescence indices, and ...
... In this study, combining metabolome and transcriptome, color related attributes and phenolic compositions of Tunisian pomegranate arils from 7 Chinese regions at same developing stage were studied. The total anthocyanin (TAC), flavonoids, and percent polymeric color (PPC) were ranged at 8.93–28.41 mg/100 g arils, 37.55–69.72 mg/100 g arils, and 3.38–21.96%, respectively. In total, 51 phenolic comp ...
... Water bamboo shoots (WBS) are popular hydrophytic plant in China with a high nutritive value. However, WBS is susceptible to postharvest lignification. The firmness, lignin content, lignin composition, activity of lignin biosynthesis-related enzymes, and expression of lignin biosynthesis-related genes in WBS were determined after postharvest storage at 20 °C or 2 °C for 15 d or 63 d, respectively. ...
... Alginates are widely used polysaccharides for biomaterials engineering, which functional properties depend on guluronic and mannuronic acid as the building blocks. In this study, enzymatically crosslinked hydrogels based on sodium alginate (Na-Alg) and the exopolysaccharide (EPS) derived from Cryptococcus laurentii 70766 with glucuronic acid residues were synthesized and characterized as a new pot ...
... Canola is an important temperate oil crop that can be severely affected by drought. Understanding the physiological and molecular mechanisms involved in canola tolerance to water deficit is essential to obtain drought-tolerant productive cultivars. To investigate the role of antioxidant response and the possible involvement of calcium-dependent protein kinases (CDPKs) in canola tolerance to drough ...
... Milk proteins and the products of their hydrolysis are characterised by high levels of biological activity. The health-promoting potential of bovine milk proteins, peptides and amino acids can be attributed to their antioxidant, anti-inflammatory and immunostimulatory properties. Milk proteins, peptides and enzymes play a role in the maintenance of prooxidant/antioxidant homeostasis in the human b ...
... High temperature (HT) severely limits rice production, but effective measures can reduce the adverse effects of HT. A 2-year experiment was conducted involving five sowing dates to investigate the effects of potassium dihydrogen phosphate (KH₂PO₄), salicylic acid (SA), and the sowing date on the physiological characteristics, yield and quality of rice subjected to HT. Under ambient HT conditions ( ...
... Soil microflora disorder has been regarded as one of the critical causes for the continuous cropping obstacles that result in stunted plant growth, serious disease incidence, and low yield and quality in crops. Pinellia ternata (Thunb.) Breit. is a traditional Chinese herb with important pharmacological values in its dried tuber. However, this plant is subject to the continuous cropping obstacles ...
... This work reports on the fabrication and performance of a new on-chip array of gold thin-film electrodes arranged into five individually addressable miniaturized electrochemical cells. Each cell shows a two-electrode configuration comprising a single working electrode and a counter/pseudo-reference electrode that is compartmentalized to be shared among all the cells of the array. Using this config ...
... Wheatgrass and barley grass are freshly sprouted leaves of wheat and barley seeds and are rich sources of phytochemicals. This study was conducted to investigate the effects of drought stress on the biochemical compounds and antioxidant activities of barley grass and wheatgrass extracts. The grass was cultivated in an organic soil growing medium with different levels of drought stress (a control w ...
abortion (animals); autoantibodies; autoimmunity; body mass index; cohort studies; confidence interval; hospitals; iodine; iodine deficiency; nutritional status; odds ratio; peroxidase; raw materials; research; risk factors; thyroid hormones; China
Abstract:
... Iodine is an essential trace element for humans and the main raw material for thyroid hormone synthesis. However, the association between iodine nutritional status and adverse pregnancy outcomes in different regions remains controversial. This single-center cohort study was focused on the association between iodine nutritional status and adverse pregnancy outcomes in Beijing, China. We enrolled 72 ...
... Amyloid β-peptide (Aβ) is a key predictor for preclinical diagnosis of Alzheimer's disease (AD) and vascular diseases. In this work, we propose a gold nanoparticle (AuNPs)-Aβ-nickel (Ni)-horseradish peroxidase (HRP) based colorimetric assay for the detection of Aβ₁₋₄₀. The consecutive binding of Aβ₁₋₄₀ to AuNPs and metal ions is designed and examined for Aβ-specific aggregation of AuNPs and the ge ...
... Drought causes severe economic losses by disturbing normal plant growth and productivity in agricultural systems. Plant-associated beneficial microorganisms have been studied to alleviate drought stress by inducing tolerance to stress. In this study, we investigated plant responses to drought stress by withholding irrigation water, as well as changes in polyphenol content, including flavonoids, in ...
... Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (Eisenia fetida) in natural soil matrix. PHE exhibited a relatively high toxicity to E. fetida in natur ...
... Antagonistic interactions among different functional guilds of nematodes have been recognized for quite some time, but the underlying explanatory mechanisms are unclear. We investigated responses of tomato (Solanum lycopersicum) to two functional guilds of nematodes—plant parasite (Meloidogyne javanica) and entomopathogens (Heterorhabditis bacteriophora, Steinernema feltiae below‐ground, and S. ca ...
... Life is fueled by multi-enzymatic tandem processes that display unmatched catalytic efficiencies owing to certain features of the biological reactors such as compartmentalization, nano-confinement, and out-of-equilibrium dynamics. With an attempt to match such natural catalytic systems, herein, we present a chemoenzymatic pH clock mediated transient assembly of a vesicular nanozyme. Distinct confi ...
... The rapid development of nanozymes for ultrasensitive detection of contaminate has resulted in considerable attention. Herein, a carboxyl- and aminopropyl-functionalized copper organophyllosilicate (Cu-CAP) was synthesized by a facile, one-pot sol–gel method. The bifunctional groups endow it with superior catalytic activity than that of natural enzyme. Besides, it possesses outstanding catalytic s ...
Muhammad Mubashar Zafar; Yufang Zhang; Muhammad Awais Farooq; Arfan Ali; Hina Firdous; Muhammad Haseeb; Sajid Fiaz; Amir Shakeel; Abdul Razzaq; Maozhi Ren
... Heat tolerance is a physiologically and genetically complex trait regulated by multiple genes. To investigate the genetic basis of heat tolerance, eight parents (five lines and three testers) and their fifteen F1 hybrids were evaluated under normal and high-temperature stress conditions for two consecutive years. Data were recorded for plant height, number of bolls, boll weight, seed cotton yield, ...
... Streptomyces strains were isolated from rhizosphere soil and evaluated for in vitro plant growth and antagonistic potential against Ralstonia solanacearum. Based on their in vitro screening, seven Streptomyces were evaluated for plant growth promotion (PGP) and biocontrol efficacy by in-planta and pot culture study. In the in-planta study, Streptomyces-treated eggplant seeds showed better germinat ...
... Lack of degradability and the accumulation of polymeric wastes increase the risk for the health of the environment. Recently, recycling of polymeric waste materials becomes increasingly important as raw materials for polymer synthesis are in short supply due to the rise in price and supply chain disruptions. As an important polymer, polyurethane (PU) is widely used in modern life, therefore, PU bi ...
... This paper aims to reduce the gap between intensified innovative process designs and the application of enzyme technologies at an industrial scale that often take full advantage of microreactor systems. Soybean and potato processing waste was used as the source of peroxidase in both the free and immobilized form for the biodegradation of the Acid Violet 109 anthraquinone dye (AV109) in a PTFE (pol ...
Escherichia coli O157; chemical species; chemiluminescence; detection limit; enzyme immunoassays; food safety; nanocomposites; peroxidase; storage quality; tap water
Abstract:
... Immunoassays based on enzyme-labeled antibodies have been widely used in the food safety field. However, the production process of enzyme-labeled antibodies is complicated and the low storage stability limits their application. Herein, antibody–horseradish peroxidase (HRP) co-assembled nanocomposites (AHC NCs) with outstanding advantages such as enhanced stability, lower cost, and substrate affini ...
... An region of ecological hazard (EHR) is an area where, as a result of intensive human economic activity, degradation of components of the natural environment has taken place, leading to the deterioration of the ecological balance. EHR management is a globalised ever-increasing challenge. To eliminate the hazardous effect of these pollutants, research has been accelerated worldwide. The current stu ...
... Biomimic nanozymes coassembled by peptides or proteins and small active molecules provide an effective strategy to design attractive nanozymes. Although some promising nanozymes have been reported, rational regulation for higher catalytic activity of biomimic nanozymes remains challenging. Hence, we proposed a novel biomimic nanozyme by encapsulating the coassembly of hemin/bovine serum albumin (B ...
... Climate change and disproportionate anthropogenic interventions, such as the excess of phytopharmaceutical products and continuous soil tillage, are jeopardizing viticulture by subjecting plants to continuous abiotic stress. One of the main physiological repercussions of abiotic stress is represented by the unbalanced redox homeostasis due to the overproduction of reactive oxygen species (ROS), ul ...
... Block copolymer vesicles with diverse functionalities and intrinsic hollow structures have received considerable attention due to their broad applications in biomedical fields, including drug delivery, bioimaging, theranostics, gene therapy, etc. However, efficient preparation of block copolymer vesicles with tunable membrane thicknesses and compositions under mild conditions is still a challenge. ...