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You searched for: Subject term promoter regions Remove constraint Subject term: promoter regions Subject term ethyl methanesulfonate Remove constraint Subject term: ethyl methanesulfonate Subject term pH Remove constraint Subject term: pH Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate

Author:
Farshad Darvishi; Jacqueline Destain; Iraj Nahvi; Philippe Thonart; Hamid Zarkesh-Esfahani
Source:
New biotechnology 2011 v.28 no.6 pp. 756-760
ISSN:
1871-6784
Subject:
Yarrowia lipolytica; bioreactors; carboxylic ester hydrolases; ethyl methanesulfonate; genes; hydrophobicity; molecular weight; mutants; mutation; oleic acid; open reading frames; pH; promoter regions; recombinant DNA; selective media; sequence analysis; temperature; value-added products; yeasts
Abstract:
... The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica t ...
DOI:
10.1016/j.nbt.2011.02.002