You searched for:
Subject term
protein microarrays
Remove constraint Subject term: protein microarrays
Text Availability
Citation in PubAg
Remove constraint Text Availability: Citation in PubAg
PubAg
Main content area
Limit your search
- Citation in PubAg[remove]78
Search
78 Search Results
« Previous |
1 - 50 of 78
|
Next »
Search Results
- Author:
- Jian Yan; Changwen Zhao; Yuhong Ma; Wantai Yang
- Source:
- Biomacromolecules 2022 v.23 no.6 pp. 2614-2623
- ISSN:
- 1526-4602
- Subject:
- chemical bonding; composite polymers; condensation reactions; detection limit; glass; hydrolysis; immunoassays; immunoglobulin G; microstructure; olefin; protein microarrays; roughness; silane; silica; surface area; transmittance
- Abstract:
- ... Compared to traditional two-dimensional (2D) biochips, three-dimensional (3D) biochips exhibit the advantages of higher probe density and detection sensitivity due to their designable surface microstructure as well as enlarged surface area. In the study, we proposed an approach to prepare a 3D protein chip by deposition of a monolayer of functionalized hollow silica nanoparticles (HSNs) on an acti ...
- DOI:
- 10.1021/acs.biomac.2c00354
- https://doi.org/10.1021/acs.biomac.2c00354
- Author:
- Tzong-Shiann Ho; Pin-Xian Du; Wen-Yu Su; Harvey M. Santos; Ya-Lan Lin; Yi-Yu Chou; Batuhan Birol Keskin; Chi Ho Pau; Guan-Da Syu
- Source:
- Biosensors & bioelectronics 2022 v.204 pp. 114067
- ISSN:
- 0956-5663
- Subject:
- Severe acute respiratory syndrome coronavirus 2; antibodies; biosensors; drug development; humoral immunity; neutralization; neutralization tests; people; protein microarrays; vaccination; vaccines
- Abstract:
- ... SARS-CoV-2 is quickly evolving from wild-type to many variants and spreading around the globe. Since many people have been vaccinated with various types of vaccines, it is crucial to develop a high throughput platform for measuring the antibody responses and surrogate neutralizing activities against multiple SARS-CoV-2 variants. To meet this need, the present study developed a SARS-CoV-2 variant ( ...
- DOI:
- 10.1016/j.bios.2022.114067
- https://doi.org/10.1016/j.bios.2022.114067
- Author:
- Angus Shiue; Jenn-Han Chen; Cheng-Yen Hsiao; Shu-Mei Chang; Kuo-Yuan Hwa; Graham Leggett
- Source:
- Journal of immunological methods 2022 v.502 pp. 113218
- ISSN:
- 0022-1759
- Subject:
- antigens; fluorescence; fluorescent dyes; glass; protein microarrays; scanners; silicon; vertebrate viruses; viruses
- Abstract:
- ... Protein microarray chips are composed of three components, these are pre-treatment substrates, surface chemical modification, and immobilizing protein on substrate surfaces. In this study, self-assembly monolayers are used for surface chemical modification. Using this method, silanization on a glass and silicon chip is achieved, forming the terminal group substrates. Modification of the substrate ...
- DOI:
- 10.1016/j.jim.2022.113218
- https://doi.org/10.1016/j.jim.2022.113218
- Author:
- Jorge Pulido; Marga García-Durán; Ricardo Fernández-Antonio; Carmen Galán; Lissette López; Carmen Vela; Ángel Venteo; Paloma Rueda; Luis A. Rivas
- Source:
- Journal of veterinary diagnostic investigation 2022 v.34 no.2 pp. 190-198
- ISSN:
- 1943-4936
- Subject:
- COVID-19 infection; blood serum; mink; nucleoproteins; protein microarrays; public health
- Abstract:
- ... During the COVID-19 pandemic, infection of farmed mink has become not only an economic issue but also a widespread public health concern. International agencies have advised the use of strict molecular and serosurveillance methods for monitoring the SARS-CoV2 status on mink farms. We developed 2 ELISAs and a duplex protein microarray immunoassay (MI), all in a double-recognition format (DR), to de ...
- DOI:
- 10.1177/10406387211057859
- https://doi.org/10.1177/10406387211057859
- Author:
- Jian Yan; Changwen Zhao; Yuhong Ma; Wantai Yang
- Source:
- Journal of materials chemistry B 2022 v.10 no.2 pp. 293-301
- ISSN:
- 2050-7518
- Subject:
- anhydrides; composite polymers; detection limit; epoxides; ethylenediamines; fluorescence; immunoassays; immunoglobulin G; microparticles; olefin; polymerization; protein microarrays; surface roughness; texture; transmittance
- Abstract:
- ... Fabrication of three-dimensional (3D) surface structures for the high density immobilization of biomolecules is an effective way to prepare highly sensitive biochips. In this work, a strategy to attach polymeric microspheres on a cyclic olefin copolymer (COC) substrate for the preparation of a 3D protein chip was developed. The COC surface was firstly functionalized by the photograft technique wit ...
- DOI:
- 10.1039/d1tb02238e
- https://doi.org/10.1039/d1tb02238e
- Author:
- Yun-Sook Lim; Han N. Mai; Lap P. Nguyen; Sang Min Kang; Dongseob Tark; Soon B. Hwang
- Source:
- journal of microbiology 2021 v.59 no.1 pp. 101-109
- ISSN:
- 1225-8873
- Subject:
- Hepatitis C virus; adenosylhomocysteinase; humans; microbiology; mutants; precipitin tests; protein microarrays; protein synthesis; ribosomes
- Abstract:
- ... Hepatitis C virus (HCV) life cycle is highly dependent on cellular proteins for viral propagation. In order to identify the cellular factors involved in HCV propagation, we previously performed a protein microarray assay using the HCV nonstructural 5A (NS5A) protein as a probe. Of ∼9,000 human cellular proteins immobilized in a microarray, adenosylhomocysteinase like 1 (AHCYL1) was among 90 protei ...
- DOI:
- 10.1007/s12275-021-0470-8
- https://doi.org/10.1007/s12275-021-0470-8
- Author:
- Wen-Yu Su; Pin-Xian Du; Harvey M. Santos; Tzong-Shiann Ho; Batuhan Birol Keskin; Chi Ho Pau; An-Ming Yang; Yi-Yu Chou; Hsi-Chang Shih; Guan-Da Syu
- Source:
- Analytical chemistry 2022 v.94 no.17 pp. 6529-6539
- ISSN:
- 1520-6882
- Subject:
- COVID-19 infection; analytical chemistry; blood serum; death; disease progression; dose response; drugs; humoral immunity; protein microarrays; viral nonstructural proteins
- Abstract:
- ... The disease progression of COVID-19 varies from mild to severe, even death. However, the link between COVID-19 severities and humoral immune specificities is not clear. Here, we developed a multiplexed spike variant protein microarray (SVPM) and utilized it for quantifying neutralizing activity, drug screening, and profiling humoral immunity. First, we demonstrated the competition between antispik ...
- DOI:
- 10.1021/acs.analchem.1c05567
- https://doi.org/10.1021/acs.analchem.1c05567
- Author:
- Pin-Xian Du; Yi-Yu Chou; Harvey M. Santos; Batuhan Birol Keskin; Miao-Hsi Hsieh; Tzong-Shiann Ho; Jiu-Yao Wang; Yi-Ling Lin; Guan-Da Syu
- Source:
- Analytical chemistry 2021 v.93 no.21 pp. 7690-7698
- ISSN:
- 1520-6882
- Subject:
- COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; analytical chemistry; biological development; biomarkers; blood serum; cross reaction; drugs; humans; influenza; nucleocapsid proteins; protein microarrays; surfactant proteins
- Abstract:
- ... Coronavirus is an enveloped RNA virus that causes mild to severe respiratory diseases in humans, including HKU1-CoV, 229E-CoV, NL63-CoV, OC43-CoV, SARS-CoV, MERS-CoV, and SARS-CoV-2. Due to the outbreak of SARS-CoV-2, it is important to identify the patients and investigate their immune responses. Protein microarray is one of the best platforms to profile the antibodies in the blood because of its ...
- DOI:
- 10.1021/acs.analchem.1c00614
- https://doi.org/10.1021/acs.analchem.1c00614
- Author:
- Joëlle De Meutter; Erik Goormaghtigh
- Source:
- Analytical chemistry 2021 v.93 no.8 pp. 3733-3741
- ISSN:
- 1520-6882
- Subject:
- Fourier transform infrared spectroscopy; analytical chemistry; least squares; prediction; protein microarrays; protein secondary structure; support vector machines
- Abstract:
- ... The paper introduces a new method designed for high-throughput protein structure determination. It is based on spotting proteins as microarrays at a density of ca. 2000–4000 samples per cm² and recording Fourier transform infrared (FTIR) spectra by FTIR imaging. It also introduces a new protein library, called cSP92, which contains 92 well-characterized proteins. It has been designed to cover as w ...
- DOI:
- 10.1021/acs.analchem.0c03677
- https://doi.org/10.1021/acs.analchem.0c03677
10. Multiomics Profiling of Alzheimer’s Disease Serum for the Identification of Autoantibody Biomarkers
- Author:
- Pablo San Segundo-Acosta; Ana Montero-Calle; August Jernbom-Falk; Miren Alonso-Navarro; Elisa Pin; Eni Andersson; Cecilia Hellström; MariCruz Sánchez-Martínez; Alberto Rábano; Guillermo Solís-Fernández; Alberto Peláez-García; Javier Martínez-Useros; María Jesús Fernández-Aceñero; Anna Månberg; Peter Nilsson; Rodrigo Barderas
- Source:
- Journal of proteome research 2021 v.20 no.11 pp. 5115-5130
- ISSN:
- 1535-3907
- Subject:
- Western blotting; autoantibodies; autoantigens; biomarkers; blood serum; databases; disease diagnosis; immunohistochemistry; luminescence; mass spectrometry; multiomics; precipitin tests; prefrontal cortex; protein microarrays; proteome; research
- Abstract:
- ... New biomarkers of Alzheimer’s disease (AD) with a diagnostic value in preclinical and prodromal stages are urgently needed. AD-related serum autoantibodies are potential candidate biomarkers. Here, we aimed at identifying AD-related serum autoantibodies using protein microarrays and mass spectrometry-based methods. To this end, an untargeted complementary screening using high-density (42,100 antig ...
- DOI:
- 10.1021/acs.jproteome.1c00630
- https://doi.org/10.1021/acs.jproteome.1c00630
- Author:
- Joëlle De Meutter; Erik Goormaghtigh
- Source:
- Analytical chemistry 2021 v.93 no.40 pp. 13441-13449
- ISSN:
- 1520-6882
- Subject:
- Fourier transform infrared spectroscopy; analytical chemistry; denaturation; protein microarrays
- Abstract:
- ... The loss of native structure is common in proteins. Among others, aggregation is one structural modification of particular importance as it is a major concern for the efficiency and safety of biotherapeutic proteins. Yet, recognizing the structural features associated with intermolecular bridging in a high-throughput manner remains a challenge. We combined here the use of protein microarrays spott ...
- DOI:
- 10.1021/acs.analchem.1c01416
- https://doi.org/10.1021/acs.analchem.1c01416
- Author:
- Chandraprakash Yamini; Govindasamy Sharmila; Chandrasekaran Muthukumaran; Kumar Pavithran; Narasimhan Manojkumar
- Source:
- Molecular biology reports 2022 v.49 no.1 pp. 629-646
- ISSN:
- 0301-4851
- Subject:
- heat; heat tolerance; mass spectrometry; molecular biology; protein microarrays; proteomics; thermal stability
- Abstract:
- ... INTRODUCTION: Thermotolerant microbes are a group of microorganisms that survive in elevated temperatures. The thermotolerant microbes, which are found in geothermal heat zones, grow at temperatures of or above 45°C. The proteins present in such microbes are optimally active at these elevated temperatures. Hence, therefore, serves as an advantage in various biotechnological applications. In the la ...
- DOI:
- 10.1007/s11033-021-06805-z
- https://doi.org/10.1007/s11033-021-06805-z
- Author:
- Elisa Baldelli; Emna El Gazzah; John Conor Moran; Kimberley A. Hodge; Zarko Manojlovic; Rania Bassiouni; John D. Carpten; Vienna Ludovini; Sara Baglivo; Lucio Crinò; Fortunato Bianconi; Ting Dong; Jeremy Loffredo; Emanuel F. Petricoin; Mariaelena Pierobon
- Source:
- Genes 2021 v.12 no.9 pp. -
- ISSN:
- 2073-4425
- Subject:
- adenocarcinoma; alleles; cell cycle; lung neoplasms; lungs; mutants; neoplasm cells; protein microarrays; therapeutics
- Abstract:
- ... KRAS mutations are one of the most common oncogenic drivers in non-small cell lung cancer (NSCLC) and in lung adenocarcinomas in particular. Development of therapeutics targeting KRAS has been incredibly challenging, prompting indirect inhibition of downstream targets such as MEK and ERK. Such inhibitors, unfortunately, come with limited clinical efficacy, and therefore the demand for developing n ...
- DOI:
- 10.3390/genes12091402
- https://doi.org/10.3390/genes12091402
14. Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort
- Author:
- Muneerah Smith; Houari B. Abdesselem; Michelle Mullins; Ti-Myen Tan; Andrew J. M. Nel; Maryam A. Y. Al-Nesf; Ilham Bensmail; Nour K. Majbour; Nishant N. Vaikath; Adviti Naik; Khalid Ouararhni; Vidya Mohamed-Ali; Mohammed Al-Maadheed; Darien T. Schell; Seanantha S. Baros-Steyl; Nur D. Anuar; Nur H. Ismail; Priscilla E. Morris; Raja N. R. Mamat; Nurul S. M. Rosli; Arif Anwar; Kavithambigai Ellan; Rozainanee M. Zain; Wendy A. Burgers; Elizabeth S. Mayne; Omar M. A. El-Agnaf; Jonathan M. Blackburn
- Source:
- Viruses 2021 v.13 no.5 pp. -
- ISSN:
- 1999-4915
- Subject:
- COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; disease severity; epitopes; humoral immunity; immunologic techniques; lifestyle; nationalities and ethnic groups; nucleocapsid proteins; protein microarrays; seroprevalence; vaccines
- Abstract:
- ... The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microa ...
- DOI:
- 10.3390/v13050786
- https://doi.org/10.3390/v13050786
- Author:
- Eirini S. Soilemetzidou; Erwin de Bruin; Kathrin Eschke; Walid Azab; Nikolaus Osterrieder; Gábor Á. Czirják; Bayarbaatar Buuveibaatar; Petra Kaczensky; Marion Koopmans; Chris Walzer; Alex D. Greenwood
- Source:
- Veterinary microbiology 2020 v.242 pp. 108605
- ISSN:
- 0378-1135
- Subject:
- Anseriformes; Equus hemionus hemionus; Equus przewalskii; Influenza A virus; antibodies; asses; birds; blood serum; ecosystems; epizootic diseases; equine influenza; hemagglutinins; horses; hosts; mules; protein microarrays; sympatry; Mongolia
- Abstract:
- ... The majority of influenza A virus strains are hosted in nature by avian species in the orders of Anseriformes and Charadriformes. A minority of strains have been able to cross species boundaries and establish themselves in novel non-avian hosts. Influenza viruses of horses, donkeys, and mules represent such successful events of avian to mammal influenza virus adaptation. Mongolia has over 3 millio ...
- DOI:
- 10.1016/j.vetmic.2020.108605
- https://doi.org/10.1016/j.vetmic.2020.108605
16. Comprehensive Analyses of the Immunoglobulin Proteome for the Classification of Glomerular Diseases
- Author:
- Jianhua Liu; Yang Li; Jiayu Dai; Baoxu Lin; Chunying Xiao; Xinpeng Zhang; Lin Luo; Tingting Wang; Xiaoying Li; Yao Yu; Shixiao Chen; Lina Wu; Yong Liu; Xiaobo Yu; Xiaosong Qin
- Source:
- Journal of proteome research 2020 v.19 no.4 pp. 1502-1512
- ISSN:
- 1535-3907
- Subject:
- biopsy; blood serum; human diseases; immunoglobulins; kidney diseases; mathematical models; microarray technology; normal distribution; patients; proteome; volunteers
- Abstract:
- ... Glomerular diseases, which are currently diagnosed using an invasive renal biopsy, encompass numerous disease subtypes that often display similar clinical manifestations even though they have different therapeutic regimes. Therefore, a noninvasive assay is needed to classify and guide the treatment of glomerular diseases. Here, we develop and apply a high-throughput and quantitative microarray pla ...
- DOI:
- 10.1021/acs.jproteome.9b00748
- https://doi.org/10.1021/acs.jproteome.9b00748
- Author:
- Yuan Li; Jianhua Hu; Jing Lei; Wentao Fan; Zhenwei Bi; Suquan Song; Liping Yan
- Source:
- Archives of virology 2021 v.166 no.4 pp. 1113-1124
- ISSN:
- 0304-8608
- Subject:
- Avian leukosis virus; Avian orthoavulavirus 1; Chicken anemia virus; Infectious bronchitis virus; Infectious bursal disease virus; Influenza A virus; antibodies; blood serum; chickens; cross reaction; enzyme-linked immunosorbent assay; hemagglutination; phosphoproteins; poultry industry; protein microarrays; rapid methods; vaccination; viral nonstructural proteins; virology
- Abstract:
- ... Avian influenza virus (AIV), Newcastle disease virus (NDV), and avian infectious bronchitis virus (IBV) inflict immense damage on the global poultry industry annually. Serological diagnostic methods are fundamental for the effective control and prevention of outbreaks caused by these viruses. In this study, a novel triplex protein microarray assay was developed and validated for the rapid and simu ...
- DOI:
- 10.1007/s00705-021-04962-x
- https://doi.org/10.1007/s00705-021-04962-x
- Author:
- Peng Chen; Zehua Zeng; Hongwu Du
- Source:
- Biochemical and biophysical research communications 2020 v.530 no.1 pp. 4-9
- ISSN:
- 0006-291X
- Subject:
- COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; humans; pharmacology; politics; protein microarrays; research; therapeutics; viruses; China
- Abstract:
- ... COVID-19 has become one of the worst epidemic in the world, currently already more than four million people have been infected, which probably co-exist with human beings, and has a significant impact on the global economy and political order. In the process of fighting against the epidemic in China, the clinical value of a variety of herbal medicines has been recognized and written into the clinic ...
- DOI:
- 10.1016/j.bbrc.2020.05.217
- https://doi.org/10.1016/j.bbrc.2020.05.217
- Author:
- Chenfang Sun; Manikkedath V. Vinayak; Shanshan Cheng; Wenping Hu
- Source:
- Analytical chemistry 2021 v.93 no.32 pp. 11305-11311
- ISSN:
- 1520-6882
- Subject:
- analytical chemistry; antigens; biocompatibility; biomarkers; condensation reactions; cross-linking reagents; gelation; glutaraldehyde; liver neoplasms; polymers; protein microarrays
- Abstract:
- ... In recent years, organic field-effect transistors (OFETs) have shown great potential for advanced protein biochips due to their inherent biocompatibility and high-throughput detectability. However, the development of OFET-based protein biochips is still at an early stage. On the one hand, single-biomarker determination is not sufficient for the diagnosis of cancer; thus, simultaneous monitoring of ...
- DOI:
- 10.1021/acs.analchem.1c02601
- https://doi.org/10.1021/acs.analchem.1c02601
- Author:
- Feng Feng; Sila Toksoz Ataca; Mingxuan Ran; Yumei Wang; Michael Breen; Thomas B. Kepler
- Source:
- Journal of proteome research 2020 v.19 no.7 pp. 2664-2675
- ISSN:
- 1535-3907
- Subject:
- antibody specificity; biomarkers; blood serum; data collection; humans; immune response; information processing; microbial detection; models; monoclonal antibodies; protein binding; protein microarrays; proteins; proteome
- Abstract:
- ... Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we describe and demonstrate a new data processing procedure, gain-scan, in which data were ...
- DOI:
- 10.1021/acs.jproteome.9b00892
- https://doi.org/10.1021/acs.jproteome.9b00892
- Author:
- Qinwen Xu; Sihong Liu; Kokouvi Kassegne; Bo Yang; Jiachen Lu; Yifan Sun; Wenli Zhong; Miaosa Zhang; Yaobao Liu; Guoding Zhu; Jun Cao; Yang Cheng
- Source:
- Parasites & vectors 2021 v.14 no.1 pp. 583
- ISSN:
- 1756-3305
- Subject:
- DNA; Escherichia coli; Plasmodium falciparum; Plasmodium ovale; T-lymphocytes; Western blotting; antibodies; blood serum; cross reaction; enzyme-linked immunosorbent assay; erythrocytes; genes; genetic variation; immunogenicity; lymphocyte proliferation; malaria; merozoites; polymerase chain reaction; protein microarrays; splenocytes; surface proteins; vaccine development; vaccines; Africa; China
- Abstract:
- ... BACKGROUND: Merozoite surface protein 1 (MSP1) plays an essential role in erythrocyte invasion by malaria parasites. The C-terminal 19-kDa region of MSP1 has long been considered one of the major candidate antigens for a malaria blood-stage vaccine against Plasmodium falciparum. However, there is limited information on the C-terminal 19-kDa region of Plasmodium ovale MSP1 (PoMSP1₁₉). This study ai ...
- DOI:
- 10.1186/s13071-021-05086-6
- https://doi.org/10.1186/s13071-021-05086-6
- Author:
- Jianlin Li; Tingting Cai; Wei Li; Weiwei Li; Laicui Song; Qianjin Li; Guangping Lv; Jialong Sun; Saisai Jiao; Siwei Wang; Yu Jin; Tiesong Zheng
- Source:
- Journal of agricultural and food chemistry 2020 v.69 no.1 pp. 528-536
- ISSN:
- 1520-5118
- Subject:
- epoxides; food chemistry; ochratoxin A; protein microarrays; silicon
- Abstract:
- ... A new protein microarray method for multiplex mycotoxin detection in parallel has been established on a stable TiO₂-modified porous silicon (PSi) surface. A typical competitive immunoassay microarray protocol has been developed for simultaneous detection of multiplex mycotoxins including aflatoxin B₁ (AFB₁), ochratoxin A (OTA), and fumonisin B₁ (FB₁) on the TiO₂-PSi surface. The epoxy groups were ...
- DOI:
- 10.1021/acs.jafc.0c06859
- https://doi.org/10.1021/acs.jafc.0c06859
- Author:
- Wenchao Li; Bowen Pan; Yang Shi; Meiying Wang; Tianjiao Han; Qing Wang; Guifang Duan; Hongzheng Fu
- Source:
- Chemical communications 2021 v.57 no.51 pp. 6308-6311
- ISSN:
- 1364-548X
- Subject:
- andrographolide; apoptosis; biological assessment; cardiotoxicity; cell lines; cell movement; enzyme activity; models; protein microarrays; surface plasmon resonance; xenotransplantation
- Abstract:
- ... Here, we describe the identification of PARP1/2 as direct binding proteins of andrographolide (Andro) using protein microarray, surface plasmon resonance (SPR), and enzyme activity assays. We then evaluated the proliferation inhibition, apoptosis, and cell migration effects of Andro on the MDA-MB-436 cell line in vitro. The final biological evaluation confirmed that Andro was a highly effective si ...
- DOI:
- 10.1039/d1cc02272e
- https://doi.org/10.1039/d1cc02272e
- Author:
- Eliane Marti; Ella N. Novotny; Iva Cvitas; Anja Ziegler; A. Douglas Wilson; Sigurbjörg Torsteinsdottir; Antonia Fettelschoss‐Gabriel; Sigridur Jonsdottir
- Source:
- Veterinary dermatology 2021 v.32 no.6 pp. 579
- ISSN:
- 0959-4493
- Subject:
- Culicoides; T-lymphocytes; allergens; alum; dermatitis; eosinophils; immune response; immunization; immunoglobulins; interleukin-13; interleukin-5; lymph; pathogenesis; protein microarrays; saliva; sweet itch; veterinary medicine; Northern European region
- Abstract:
- ... Culicoides hypersensitivity (CH) is a strongly pruritic dermatitis of horses, characterised histologically by mixed perivascular to diffuse cellular infiltrates of mononuclear cells and eosinophils, and caused by helper T cell (Th)2, immunoglobulin (Ig)E‐mediated reactions to antigens present in the saliva of biting Culicoides midges. As treatment of CH is still not satisfactory, a better knowledg ...
- DOI:
- 10.1111/vde.13042
- https://doi.org/10.1111/vde.13042
- Author:
- Guang Song; Emily M. Lee; Jianbo Pan; Miao Xu; Hee-Sool Rho; Yichen Cheng; Nadia Whitt; Shu Yang; Jennifer Kouznetsova; Carleen Klumpp-Thomas; Samuel G. Michael; Cedric Moore; Ki-Jun Yoon; Kimberly M. Christian; Anton Simeonov; Wenwei Huang; Menghang Xia; Ruili Huang; Madhu Lal-Nag; Hengli Tang; Wei Zheng; Jiang Qian; Hongjun Song; Guo-li Ming; Heng Zhu
- Source:
- Genomics, proteomics & bioinformatics 2021 v.19 no.1 pp. 108-122
- ISSN:
- 1672-0229
- Subject:
- Dengue virus; Zika virus; data collection; drugs; genomics; humans; multiomics; pathogenesis; proteasome endopeptidase complex; protein microarrays; protein-protein interactions; proteomics; virus replication
- Abstract:
- ... The Zika virus (ZIKV) and dengue virus (DENV) flaviviruses exhibit similar replicative processes but have distinct clinical outcomes. A systematic understanding of virus–host protein–protein interaction networks can reveal cellular pathways critical to viral replication and disease pathogenesis. Here we employed three independent systems biology approaches toward this goal. First, protein array an ...
- DOI:
- 10.1016/j.gpb.2020.06.016
- https://doi.org/10.1016/j.gpb.2020.06.016
- Author:
- Christian P. Moritz; Oda Stoevesandt; Yannick Tholance; Jean-Philippe Camdessanché; Jean-Christophe Antoine
- Source:
- New biotechnology 2021 v.60 pp. 168-172
- ISSN:
- 1871-6784
- Subject:
- antigens; autoantibodies; bacteriophages; biotechnology; blood serum; mass spectrometry; patients; peripheral nervous system diseases; precipitin tests; protein microarrays; risk
- Abstract:
- ... Autoimmune diseases are frequently associated with autoantibodies. Recently, large sets of autoantibody-targeted antigens (“autoantigen-omes”) of patient and control sera have been revealed, enabling autoantigen-omic approaches. However, statistical standards for defining such autoantigen-omes are lacking. The z-score indicates how many standard deviations an antigen reactivity of a given sample i ...
- DOI:
- 10.1016/j.nbt.2020.08.007
- https://doi.org/10.1016/j.nbt.2020.08.007
- Author:
- Muriel Bonnet; Julien Soulat; Joanna Bons; Stéphanie Léger; Leanne De Koning; Christine Carapito; Brigitte Picard
- Source:
- Food chemistry 2020 v.317 pp. 126376
- ISSN:
- 0308-8146
- Subject:
- beef; biomarkers; cattle; longissimus muscle; marbling; mass spectrometry; monitoring; protein microarrays; proteins; proteomics
- Abstract:
- ... We and others have identified biomarker candidates of tenderness or marbling, two major attributes of bovine meat-eating qualities for consumers’ satisfaction. In this study, Reverse Phase Protein Arrays (RPPA) and targeted mass spectrometry assays using Parallel Reaction Monitoring (PRM) were developed to test whether 10 proteins pass the sequential qualification and verification steps of the cha ...
- DOI:
- 10.1016/j.foodchem.2020.126376
- https://doi.org/10.1016/j.foodchem.2020.126376
- Author:
- Joëlle De Meutter; Erik Goormaghtigh
- Source:
- Analytical chemistry 2020 v.93 no.3 pp. 1561-1568
- ISSN:
- 1520-6882
- Subject:
- Fourier transform infrared spectroscopy; algorithms; analytical chemistry; energy; geometry; hydrogen bonding; prediction; protein microarrays; protein secondary structure
- Abstract:
- ... Obtaining protein secondary structure content from high-resolution structures requires definitions and thresholds for the various parameters involved, typically hydrogen bond energy or length/angle and backbone φ/ψ angles. Several definitions are currently used and can have a profound impact on secondary structure content. Fourier transform infrared (FTIR) spectroscopy has its own sensitivity to m ...
- DOI:
- 10.1021/acs.analchem.0c03943
- https://doi.org/10.1021/acs.analchem.0c03943
- Author:
- Dragana Robajac; Martina Križáková; Romana Masnikosa; Goran Miljuš; Miloš Šunderić; Olgica Nedić; Jaroslav Katrlík
- Source:
- International journal of biological macromolecules 2020 v.144 pp. 932-937
- ISSN:
- 0141-8130
- Subject:
- biomarkers; cell growth; colon; colorectal neoplasms; diagnostic techniques; glycosylation; mannose; membrane proteins; patients; protein microarrays; receptors; screening; sialic acid; solubilization; somatomedins
- Abstract:
- ... Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation. Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones. Usually, only small quantities of isolated receptors are available thus there is a need of suitable assay to study rece ...
- DOI:
- 10.1016/j.ijbiomac.2019.09.170
- https://doi.org/10.1016/j.ijbiomac.2019.09.170
- Author:
- Yue Wu; Xudan Wu; Jinxiu Hou; Xiongnan Chen; Xiaobo Huang; Bin Zhou
- Source:
- BMC veterinary research 2020 v.16 no.1 pp. 57
- ISSN:
- 1746-6148
- Subject:
- Ungulate protoparvovirus 1; antibodies; antibody detection; antigens; blood serum; detection; dyes; enzyme-linked immunosorbent assay; financial economics; glass; magnetism; mixed infection; neutralization; nickel; pathogens; protein microarrays; recombinant proteins; reproductive disorders; sampling; swine; swine diseases; veterinary medicine
- Abstract:
- ... BACKGROUND: PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), ...
- DOI:
- 10.1186/s12917-020-02280-z
- https://doi.org/10.1186/s12917-020-02280-z
- Author:
- Kennedy Mwai; Nelson Kibinge; James Tuju; Gathoni Kamuyu; Rinter Kimathi; James Mburu; Emily Chepsat; Lydia Nyamako; Timothy Chege; Irene Nkumama; Samson Kinyanjui; Eustasius Musenge; Faith Osier
- Source:
- Computational and Structural Biotechnology Journal 2021 v.19 pp. 2518-2525
- ISSN:
- 2001-0370
- Subject:
- Internet; biotechnology; detection limit; experimental design; humans; infectious diseases; protein microarrays; proteome; vaccine development
- Abstract:
- ... Protein microarrays are versatile tools for high throughput study of the human proteome, but systematic and non-systematic sources of bias constrain optimal interpretation and the ultimate utility of the data. Published guidelines to limit technical variability whilst maintaining important biological variation favour DNA-based microarrays that often differ fundamentally in their experimental desig ...
- DOI:
- 10.1016/j.csbj.2021.04.044
- https://doi.org/10.1016/j.csbj.2021.04.044
- Author:
- Masahiro Watanabe; Takao Toyomura; Mayuko Tomiyama; Hidenori Wake; Keyue Liu; Kiyoshi Teshigawara; Hideo Takahashi; Masahiro Nishibori; Shuji Mori
- Source:
- Molecular biology reports 2020 v.47 no.9 pp. 7153-7159
- ISSN:
- 0301-4851
- Subject:
- Toll-like receptor 4; advanced glycation end products; apoptosis; gene expression; inflammation; lipopolysaccharides; molecular biology; nucleoproteins; protein microarrays
- Abstract:
- ... Previously, we found that advanced glycation endproducts (AGEs) directly interact with tumor necrosis factor (TNF)-like weak inducer of apoptosis, a cytokine that controls inflammation, and that this interaction inhibited its action. This finding raised the novel possibility that AGEs alter the function of other cytokines through direct interaction. To investigate this possibility, we performed co ...
- DOI:
- 10.1007/s11033-020-05783-y
- https://doi.org/10.1007/s11033-020-05783-y
- Author:
- Surender Khurana; Megan Hahn; Laura Klenow; Hana Golding
- Source:
- Viruses 2020 v.12 no.10 pp. -
- ISSN:
- 1999-4915
- Subject:
- Human immunodeficiency virus 1; Orthomyxoviridae; clinical trials; evolution; hemagglutinins; humans; influenza; influenza vaccines; messenger RNA; monoclonal antibodies; neutralization; pituitary gland; protein microarrays; therapeutics
- Abstract:
- ... Broadly neutralizing monoclonal antibodies (bNAbs) against conserved domains in the influenza hemagglutinin are in clinical trials. Several next generation influenza vaccines designed to elicit such bNAbs are also in clinical development. One of the common features of the isolated bNAbs is the use of restricted IgVH repertoire. More than 80% of stem-targeting bNAbs express IgVH1-69, which may indi ...
- DOI:
- 10.3390/v12101140
- https://doi.org/10.3390/v12101140
- Author:
- Florian Klemm; Roeltje R. Maas; Robert L. Bowman; Mara Kornete; Klara Soukup; Sina Nassiri; Jean-Philippe Brouland; Christine A. Iacobuzio-Donahue; Cameron Brennan; Viviane Tabar; Philip H. Gutin; Roy T. Daniel; Monika E. Hegi; Johanna A. Joyce
- Source:
- Cell 2020 v.181 no.7 pp. 1643-1660.e17
- ISSN:
- 0092-8674
- Subject:
- T-lymphocytes; brain; brain neoplasms; flow cytometry; glioma; immunity; macrophages; metastasis; neuroglia; neutrophils; protein microarrays; sequence analysis; transcription (genetics)
- Abstract:
- ... Brain malignancies encompass a range of primary and metastatic cancers, including low-grade and high-grade gliomas and brain metastases (BrMs) originating from diverse extracranial tumors. Our understanding of the brain tumor microenvironment (TME) remains limited, and it is unknown whether it is sculpted differentially by primary versus metastatic disease. We therefore comprehensively analyzed th ...
- DOI:
- 10.1016/j.cell.2020.05.007
- https://doi.org/10.1016/j.cell.2020.05.007
- Author:
- Qian Xiao; Zhenwei Bi; Lu Yao; Jing Lei; Yan Yan; Jiyong Zhou; Liping Yan
- Source:
- Avian pathology 2019 v.48 no.6 pp. 528-536
- ISSN:
- 1465-3338
- Subject:
- Influenza A virus; antibody detection; avian influenza; blood serum; chickens; ducks; enzyme-linked immunosorbent assay; epidemiological studies; hemagglutination; hemagglutinins; monoclonal antibodies; nucleoproteins; protein microarrays; rapid methods; serodiagnosis; viral antigens; zoonoses
- Abstract:
- ... Avian influenza virus (AIV) can cause serious zoonotic disease, thereby threatening the poultry industry and human health. An efficient and rapid detection approach is crucial to prevent and control the spread of avian influenza. In this study, a novel protein microarray was developed. Haemagglutinin proteins of H5 and H7 subtypes and nucleoprotein (NP) were purified and spotted onto the initiator ...
- DOI:
- 10.1080/03079457.2019.1634791
- https://doi.org/10.1080/03079457.2019.1634791
- Author:
- Keun Na; Minjoo Kim; Chae-Yeon Kim; Jong-Sun Lim; Jin-Young Cho; Heon Shin; Hyo Jin Lee; Byeong Jun Kang; Dai Hoon Han; Hoguen Kim; Ja-Hyun Baik; Magdalena Swiatek-de Lange; Johann Karl; Young-Ki Paik
- Source:
- Journal of proteome research 2020 v.19 no.12 pp. 4867-4883
- ISSN:
- 1535-3907
- Subject:
- apoptosis; carboxylesterase; cell cycle; cell growth; cell lines; enzyme activity; flow cytometry; fluorescence; gel electrophoresis; gene ontology; glycosylation; hepatoma; humans; liver; mass spectrometry; neoplasm cells; protein microarrays; proteome; quantitative polymerase chain reaction; research; serine; site-directed mutagenesis; transcriptome; transfection
- Abstract:
- ... We previously reported that human carboxylesterase 1 (CES1), a serine esterase containing a unique N-linked glycosyl group at Asn79 (N79 CES1), is a candidate serological marker of hepatocellular carcinoma (HCC). CES1 is normally present at low-to-undetectable levels in normal human plasma, HCC tumors, and major liver cancer cell lines. To investigate the potential mechanism underlying the suppres ...
- DOI:
- 10.1021/acs.jproteome.0c00787
- https://doi.org/10.1021/acs.jproteome.0c00787
- Author:
- Akiko Eto; Masanori Fujita; Yasumasa Nishiyama; Tomoya Saito; Douglas M. Molina; Shigeru Morikawa; Masayuki Saijo; Yasuhiko Shinmura; Yasuhiro Kanatani
- Source:
- Vaccine 2019 v.37 no.44 pp. 6588-6593
- ISSN:
- 0264-410X
- Subject:
- Vaccinia virus; adults; antibodies; antibody formation; bioterrorism; blood serum; immunodominant epitopes; immunogenicity; neutralization; protein microarrays; smallpox; vaccination; vaccines; virion
- Abstract:
- ... Concerns about bioterrorism and outbreaks of zoonotic orthopoxvirus require safe and efficacious smallpox vaccines. We previously reported the clinical efficacy and safety profiles of LC16m8, a live, attenuated, cell culture-derived, smallpox vaccine, examined in over 3000 healthy Japanese adults with various vaccination histories. In this study, serum of approximately 200 subjects pre and post LC ...
- DOI:
- 10.1016/j.vaccine.2019.09.006
- https://doi.org/10.1016/j.vaccine.2019.09.006
- Author:
- Kathryn F.A. Clancy; Sebastien Dery; Veronique Laforte; Prasad Shetty; David Juncker; Dan V. Nicolau
- Source:
- Biosensors & bioelectronics 2019 v.130 pp. 397-407
- ISSN:
- 0956-5663
- Subject:
- additives; biosensors; glass; hydrophilicity; hydrophobicity; immunoglobulin G; protein microarrays; silane; solutes
- Abstract:
- ... The uniformity of the protein patterns, their shape, and the contrast between the fluorescence signal of the pattern and the background, critically modulate the quantitative accuracy of the microarray-derived data. While significant research focused of the identification of the factors that impact the protein microarray patterns, these studies usually have focused on the optimization of one set of ...
- DOI:
- 10.1016/j.bios.2018.09.027
- https://doi.org/10.1016/j.bios.2018.09.027
- Author:
- Fang Yu; Hui Li; Wei Sun; Danke Xu; Fuchu He
- Source:
- RSC advances 2019 v.9 no.17 pp. 9762-9768
- ISSN:
- 2046-2069
- Subject:
- beta-lactoglobulin; bovine serum albumin; casein; dissociation; fluorescence; lactalbumin; lactoferrin; oligonucleotides; protein microarrays; scanners; screening; single-stranded DNA; systematic evolution of ligands by exponential enrichment
- Abstract:
- ... We report a novel method for the efficient screening of aptamers from a complex ssDNA library based on a microarray chip, which was named Microarray-SELEX. In this method, the target protein (lactoferrin) and negative proteins (α-lactalbumin, β-lactoglobulin, bovine serum albumin, and casein) were each dotted and immobilized on a slide to form a protein microarray. Moreover, the library was added ...
- DOI:
- 10.1039/c8ra09232j
- https://doi.org/10.1039/c8ra09232j
- Author:
- Ahmed Faik; Michael Held
- Source:
- Plant science 2019 v.286 pp. 49-56
- ISSN:
- 0168-9452
- Subject:
- automation; biosynthesis; cell walls; complementary DNA; desorption; glycosyltransferases; ionization; lipids; liquids; mass spectrometry; membrane proteins; oligosaccharides; polysaccharides; protein microarrays; protein-protein interactions; transcription (genetics); translation (genetics)
- Abstract:
- ... Progress in the functional biochemical analysis of plant glycosyltransferases (GTs) has been slow because plant GTs are generally membrane proteins, operate as part of larger, multimeric complexes, and utilize a vast complexity of substrate acceptors. Therefore, the field would benefit from development of adequate high throughput expression as well as product detection and characterization techniq ...
- DOI:
- 10.1016/j.plantsci.2019.04.025
- https://doi.org/10.1016/j.plantsci.2019.04.025
- Author:
- Shuxian Sun; Xiuping Zhang; Jiayi Ma; Cheng Ni; Xiao Ying; Ji Wang; Lingru Li; Wenlin Yuan; Xujun Heng; Jing Xia
- Source:
- Journal of traditional Chinese medical sciences 2019 v.6 no.1 pp. 67-74
- ISSN:
- 2095-7548
- Subject:
- Oriental traditional medicine; blood proteins; blood serum; cold; decline; energy metabolism; gene expression regulation; human population; protein composition; protein microarrays; protein synthesis; quality of life; transcription (genetics)
- Abstract:
- ... Based on the theory of traditional Chinese medicine (TCM) and pre-epidemiological investigation, Professor Qi Wang classified the entire human population into nine constitutions and put forward the theory of “Nine-Constitution Medicine.” Among these constitutions, the main feature of the yang-deficiency constitution (YADC) is intolerance of the cold, which has been proven to reduce quality of life ...
- DOI:
- 10.1016/j.jtcms.2019.02.002
- https://doi.org/10.1016/j.jtcms.2019.02.002
- Author:
- Yan Wang; Mingming Dong; Mingliang Ye
- Source:
- Electrophoresis 2019 v.40 no.16-17 pp. 2125-2128
- ISSN:
- 0173-0835
- Subject:
- aldehydes; biosensors; chemical bonding; drugs; electrophoresis; hydrazides; hydrazones; oxidation; protein microarrays; proteinases; recombinant proteins; screening; serine; synthetic peptides; tyrosine
- Abstract:
- ... Many biotechniques including protein microarray, drug screening, biosensors rely on the immobilization of recombinant proteins on the solid supports. It is well known that random orientation of the immobilized proteins could impair their biologic functions. Thus, it is very important to develop new site‐specific immobilization approach. In this study, we presented a chemoenzymatic approach for sit ...
- DOI:
- 10.1002/elps.201800528
- https://doi.org/10.1002/elps.201800528
- Author:
- Raúl Manzano-Román; Manuel Fuentes
- Source:
- Journal of proteomics 2019 v.198 pp. 27-35
- ISSN:
- 1874-3919
- Subject:
- cell physiology; molecular biology; nucleic acids; peptides; protein microarrays; protein-protein interactions; proteins; proteome; proteomics; therapeutics
- Abstract:
- ... Understanding the dynamic of the proteome is a critical challenge because it requires high sensitive methodologies in high-throughput formats in order to decipher its modifications and complexity. While molecular biology provides relevant information about cell physiology that may be reflected in post-translational changes, High-Throughput (HT) experimental proteomic techniques are essential to pr ...
- DOI:
- 10.1016/j.jprot.2018.12.007
- https://doi.org/10.1016/j.jprot.2018.12.007
- Author:
- Yue Wu; Xudan Wu; Jing Chen; Jingfei Hu; Xiaobo Huang; Bin Zhou
- Source:
- BMC veterinary research 2020 v.16 no.1 pp. 162
- ISSN:
- 1746-6148
- Subject:
- Betaarterivirus suid 1; Bluetongue virus; Classical swine fever virus; Infectious bronchitis virus; Japanese encephalitis virus; Ungulate protoparvovirus 1; Western blotting; antibodies; blood serum; cross reaction; detection; enzyme-linked immunosorbent assay; glass; mixed infection; pathogens; plasmids; protein microarrays; proteins; research; ruminants; sampling; swine; swine diseases; veterinary medicine; viruses; China
- Abstract:
- ... BACKGROUND: At present, pig industry in China is faced with the complex situation of mixed infection caused by multiple pathogens. It is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies. Biochip array technology has made it possible to screen thousands of samples simultaneously; it has been twice named as one of the top 10 scien ...
- DOI:
- 10.1186/s12917-020-02375-7
- https://doi.org/10.1186/s12917-020-02375-7
- Author:
- Andrew C. Petersen; Jeannett M. Clampitt; F. Chris Minion
- Source:
- Veterinary microbiology 2019 pp. -
- ISSN:
- 0378-1135
- Subject:
- Escherichia coli; Mycoplasma hyopneumoniae; adhesins; antibodies; antigen-antibody reactions; codons; enzyme-linked immunosorbent assay; mycoplasmosis; polymerase chain reaction; protein microarrays; serology; surface proteins; swine
- Abstract:
- ... Pigs harbor several different species of mycoplasmas, of which Mycoplasma hyopneumoniae presents the most significant economic impact on the swine industry. While ELISAs are the predominant diagnostic assay to measure antibody responses during infection with M. hyopneumoniae, the assay itself is only a rough estimate of the total antibody response. It lends little information on pathogen-wide anti ...
- DOI:
- 10.1016/j.vetmic.2019.02.010
- https://doi.org/10.1016/j.vetmic.2019.02.010
- Author:
- Ebru Demirel Sezer; Latife Merve Oktay; Elif Karadadaş; Hikmet Memmedov; Nur Selvi Gunel; Eser Sözmen
- Source:
- Journal of medicinal food 2019 v.22 no.11 pp. 1118-1126
- ISSN:
- 1557-7600
- Subject:
- 2,5-dihydroxybenzoic acid; Vaccinium myrtillus; antineoplastic activity; antineoplastic agents; antioxidant activity; antioxidants; apoptosis; blueberries; cytotoxicity; diet; flow cytometry; fruit extracts; kaempferol; liquid chromatography; neoplasms; oxidants; oxidative stress; polyphenols; protein composition; protein microarrays; proteins; quercetin; tandem mass spectrometry; therapeutics
- Abstract:
- ... In recent years, natural products gained popularity with their anti-inflammatory and antioxidant effects mediated by chemical compounds within their composition. Study results offering them as palliative therapy options in cancer or as anticancer agents with high levels of cytotoxicity brought a new approach to combine cancer treatment protocols with these products. From a different perspective, e ...
- DOI:
- 10.1089/jmf.2019.0098
- https://doi.org/10.1089/jmf.2019.0098
47. Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins
- Author:
- Janine Schulte-Zweckel; Tabea Schneidewind; Jose Luis Abad; Andreas Brockmeyer; Petra Janning; Gemma Triola
- Source:
- Chemical communications 2018 v.54 no.97 pp. 13742-13745
- ISSN:
- 1364-548X
- Subject:
- apoptosis; autophagy; ceramides; chemical reactions; inflammation; mass spectrometry; protein microarrays; proteins; proteomics
- Abstract:
- ... Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profilin ...
- DOI:
- 10.1039/c8cc05691a
- https://doi.org/10.1039/c8cc05691a
48. Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19)
- Author:
- Elvira Gonzalez de Mejia; Candice Mazewski; Diego Luna-Vital; Mark Berhow
- Source:
- Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
- ISSN:
- 2475-2991
- Subject:
- acclimation; animal models; anthocyanins; anti-inflammatory activity; azoxymethane; body weight; carcinogenesis; colon; colorectal neoplasms; delphinidin; dextran; disease control; enzyme-linked immunosorbent assay; freeze drying; gallic acid; gene expression; histology; immune response; inflammation; lentils; mice; neoplasm cells; placebos; polyphenols; protein microarrays; sodium sulfate; spleen
- Abstract:
- ... The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model. BL was obtained from an overnight soak of organic beluga black lentils (1:2 w/v) in water, filtered, frozen, and freeze- ...
- DOI:
- 10.1093/cdn/nzz030.OR04-06-19
- https://doi.org/10.1093/cdn/nzz030.OR04-06-19
49. Effect of nutritional supplement on bone marrow-derived mesenchymal stem cells from aplastic anaemia
- Author:
- Shihua Luo; Yinghai Chen; Lifen Zhao; Xia Qi; Xiaoyan Miao; Huimin Zhou; Li Jia
- Source:
- British journal of nutrition 2018 v.119 no.7 pp. 748-758
- ISSN:
- 1475-2662
- Subject:
- X-radiation; adipocytes; amino acids; anemia; animal models; bioactive properties; blood composition; blood serum; bone density; bone formation; bone marrow; chloramphenicol; cyclophosphamide; dietary supplements; dose response; gene expression regulation; hematopoiesis; hematopoietic stem cells; intragastric administration; mesenchymal stromal cells; nucleotides; nutritional support; protein microarrays; protein synthesis; rats; vitamins
- Abstract:
- ... Aplastic anaemia (AA) is characterised by pancytopenia resulting from a marked reduction in haemopoietic stem cells (HSC). The regulation of haemopoiesis depends on the interaction between HSC and various cells of the bone marrow (BM) microenvironment, including BM-derived mesenchymal stromal cells (BMSC). The purpose of this study was to analyse the biological effect of nutritional supplement (NS ...
- DOI:
- 10.1017/S0007114518000399
- https://doi.org/10.1017/S0007114518000399
- Author:
- S. Dobroiu; F.C.M.J.M. van Delft; J. Aveyard-Hanson; Prasad Shetty; D.V. Nicolau
- Source:
- Biosensors & bioelectronics 2019 v.123 pp. 251-259
- ISSN:
- 0956-5663
- Subject:
- biosensors; diagnostic techniques; fluorescence; fluorescent dyes; kinesin; molecular biology; platinum; protein microarrays; screening; silica; silicon
- Abstract:
- ... Continuous improvements of the fluorescence-based sensitivity and specificity, required for high throughput screening, diagnostics, and molecular biology studies, are usually addressed by better readout systems, or better reporting elements. However, while Fluorescence Interference Contrast (FLIC), which modulates the fluorescence by materials-based parameters, has been used for decades to measure ...
- DOI:
- 10.1016/j.bios.2018.09.009
- https://doi.org/10.1016/j.bios.2018.09.009