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You searched for: Subject term protein microarrays Remove constraint Subject term: protein microarrays
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1. Generation of Arabidopsis protein chips for antibody and serum screening

Author:
Kersten, B.; Feilner, T.; Kramer, A.; Wehrmeyer, S.; Possling, A.; Witt, I.; Zanor, M.I.; Stracke, R.; Lueking, A.; Kreutzberger, J.
Source:
Plant molecular biology 2003 v.52 no.5 pp. 999-1010
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; plant proteins; proteomics; protein composition; analytical methods; complementary DNA; cDNA libraries; monoclonal antibodies; antiserum; transcription factors; immunochemistry
Abstract:
... Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several applications on protein chips have been described, but to our knowledge no studi ...
PubMed:
14558660

2. Controlled protein assembly on a switchable surface

Author:
Ying Liu; Li Mu; Baohong Liu; Song Zhang; Pengyuan Yang; Jilie Kong
Source:
Chemical communications 2004 v. no.10 pp. 1194-1195
ISSN:
1364-548X
Subject:
biocomposites; biosensors; chemical compounds; chemical reactions; protein microarrays
Abstract:
... The strategy presented in this work supplies a general method of controlling protein assembly on a switchable low-density SAM, which may open a new way to design functional biocomposite films for biosensors or protein chips. ...
DOI:
10.1039/b400776j

3. Activity-based fingerprinting and inhibitor discovery of cysteine proteases in a microarray

Author:
Mahesh Uttamchandani; Kai Liu; Resmi C. Panicker; Shao Q. Yao
Source:
Chemical communications 2007 v. no.15 pp. 1518-1520
ISSN:
1364-548X
Subject:
chemical compounds; chemical reactions; cysteine proteinases; gels; peptides; protein microarrays
Abstract:
... A panel of 20 peptide vinyl sulfone probes has been synthesized and used to generate activity-based fingerprinting profiles of cysteine proteases in both gel- and microarray-based formats; the inhibitor fingerprints of representative small molecule inhibitors targeted against 4 cysteine proteases were also obtained, in high-throughput, using the same protein microarray platform. ...
DOI:
10.1039/b702826a

4. Arabidopsis Protein Microarrays for the High-Throughput Identification of Protein-Protein Interactions

Author:
Sorina C. Popescu; Michael Snyder; Savithramma P. Dinesh-Kumar
Source:
Plant signaling & behavior 2007 v.2 no.5 pp. 416-420
ISSN:
1559-2324
Subject:
Arabidopsis; calmodulin; humans; plant proteins; protein microarrays; protein-protein interactions; yeasts
Abstract:
... Protein microarray technology has emerged as a powerful new approach for the study of thousands of proteins simultaneously. Protein microarrays have been used for a wide variety of applications for the human and yeast systems. In a recent study, we demonstrated that Arabidopsis functional protein microarrays can be generated and employed to characterize the function of plant proteins. The arrayed ...
DOI:
10.4161/psb.2.5.4416
PubMed:
19704619
PubMed Central:
PMC2634232

5. Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays

Author:
Popescu, Sorina C.; Popescu, George V.; Bachan, Shawn; Zhang, Zimei; Seay, Montrell; Gerstein, Mark; Snyder, Michael; Dinesh-Kumar, S.P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.11 pp. 4730-4735
ISSN:
0027-8424
Subject:
Arabidopsis thaliana; protein binding; proteome; open reading frames; protein-protein interactions; calmodulin; plant proteins
Abstract:
... Calmodulins (CaMs) are the most ubiquitous calcium sensors in eukaryotes. A number of CaM-binding proteins have been identified through classical methods, and many proteins have been predicted to bind CaMs based on their structural homology with known targets. However, multicellular organisms typically contain many CaM-like (CML) proteins, and a global identification of their targets and specifici ...
DOI:
10.1073/pnas.0611615104
PubMed:
17360592
PubMed Central:
PMC1838668

6. Soft landed protein voltammetry

Author:
Federico Pepi; Andreina Ricci; Alessandra Tata; Gabriele Favero; Marco Frasconi; Stefania Delle Noci; Franco Mazzei
Source:
Chemical communications 2007 v. no.33 pp. 3494-3496
ISSN:
1364-548X
Subject:
chemical compounds; chemical reactions; electrodes; electronics; protein microarrays
Abstract:
... The present work illustrates a new method: soft landed protein voltammetry (SLPV); this experimental procedure is based on the coupling of ion soft landing with a voltammetric technique and allows the electrode surface to be functionalized with biologically active molecules, thus opening up numerous new perspectives ranging from molecular electronics to protein chips. ...
DOI:
10.1039/b705668k

7. Comparison of the backbone dynamics of a natural and a consensus designed 3-TPR domain

Author:
Jarymowycz, Virginia A.; Cortajarena, Aitziber L.; Regan, Lynne; Stone, Martin J.
Source:
Journal of biomolecular NMR 2008 v.41 no.3 pp. 169-178
ISSN:
0925-2738
Subject:
acids; design; helix-turn-helix motifs; journals; protein microarrays; proteins; tandem repeat sequences; tetratricopeptide repeats
Abstract:
... The tetratricopeptide repeat (TPR) is a 34-amino acid helix-turn-helix motif that occurs in tandem arrays in numerous proteins. Here we compare the backbone dynamics of a natural 3-repeat TPR domain, from the protein UBP, with the behavior of a designed protein CTPR3, which consists of three identical consensus TPR units. Although the three tandem TPR repeats in both CTPR3 and UBP behave as a sing ...
DOI:
10.1007/s10858-008-9250-6

8. Human mammary progenitor cell fate decisions are products of interactions with combinatorial microenvironments

Author:
Mark A. LaBarge; Celeste M. Nelson; Rene Villadsen; Agla Fridriksdottir; Jason R. Ruth; Martha R. Stampfer; Ole W. Petersen; Mina J. Bissell
Source:
Integrative biology 2009 v.1 no.1 pp. 70-79
ISSN:
1757-9708
Subject:
adults; breasts; cell culture; genome; homeostasis; mammary glands; physical properties; progeny; protein microarrays; proteins; stem cells
Abstract:
... In adult tissues, multi-potent progenitor cells are some of the most primitive members of the developmental hierarchies that maintain homeostasis. That progenitors and their more mature progeny share identical genomes, suggests that fate decisions are directed by interactions with extrinsic soluble factors, ECM, and other cells, as well as physical properties of the ECM. To understand regulation o ...
DOI:
10.1039/b816472j
PubMed:
20023793
PubMed Central:
PMC2933184

9. Integrated analysis of co-expressed MAP kinase substrates in Arabidopsis thaliana

Author:
Sorina C. Popescu; George V. Popescu; Michael P. Snyder; Savithramma P. Dinesh-Kumar
Source:
Plant signaling & behavior 2009 v.4 no.6 pp. 524-527
ISSN:
1559-2324
Subject:
Arabidopsis thaliana; Nicotiana benthamiana; cell death; enzyme substrates; gene expression; genes; mitogen-activated protein kinase; phenotype; phosphorylation; protein microarrays; proteins; resistance mechanisms; signal transduction; stress response
Abstract:
... MAP kinase (MAPK) signal transduction cascades are conserved eukaryotic pathways that modulate stress responses and developmental processes. In a recent report we have identified novel Arabidopsis MAPKK/MAPK/Substrate signaling pathways using microarrays containing 2,158 unique Arabidopsis proteins. Subsequently, several WRKY and TGA targets phosphorylated by MAPKs were verified in planta. We have ...
DOI:
10.4161/psb.4.6.8576
PubMed:
19816141
PubMed Central:
PMC2688301

10. DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips

Author:
Mei Hu; Yao He; Shiping Song; Juan Yan; Hao-Ting Lu; Li-Xing Weng; Lian-Hui Wang; Chunhai Fan
Source:
Chemical communications 2010 v.46 no.33 pp. 6126-6128
ISSN:
1364-548X
Subject:
biomarkers; chemical bonding; chemical communication; chemical compounds; chemical reactions; fluorescence; fluorescent dyes; neoplasms; protein microarrays; quantum dots; streptavidin
Abstract:
... A DNA-bridged strategy is used to facilely conjugate streptavidin (STV) to luminescent semiconductor quantum dots (QDs), which leads to convenient and stable QD–DNA–biotin–STV conjugates that serve as fluorescent nanoprobes for ultrasensitive detection of cancer biomarkers with a microfluidic protein chip. ...
DOI:
10.1039/c0cc01608j

11. An integrated micro-electro-fluidic and protein arraying system for parallel analysis of cell responses to controlled microenvironments

Author:
Zhizhong Yin; Sheng-Ce Tao; Raymond Cheong; Heng Zhu; Andre Levchenko
Source:
Integrative biology 2010 v.2 no.9 pp. 416-423
ISSN:
1757-9708
Subject:
cellular microenvironment; dielectrophoresis; electrodes; endothelial cells; extracellular matrix; glass; ligands; liquids; polydimethylsiloxane; protein microarrays; transcription factor NF-kappa B; tumor necrosis factors
Abstract:
... Living cells have evolved sophisticated signaling networks allowing them to respond to a wide array of external stimuli. Microfluidic devices, facilitating the analysis of signaling networks through precise definition of the cellular microenvironment often lack the capacity of delivering multiple combinations of different signaling cues, thus limiting the throughput of the analysis. To address thi ...
DOI:
10.1039/c0ib00017e
PubMed:
20714638
PubMed Central:
PMC3902009

12. Aryl diazonium salts: a new class of coupling agents for bonding polymers, biomacromolecules and nanoparticles to surfaces

Author:
Samia Mahouche-Chergui; Sarra Gam-Derouich; Claire Mangeney; Mohamed M. Chehimi
Source:
Chemical Society reviews 2011 v.40 no.7 pp. 4143-4166
ISSN:
1460-4744
Subject:
adhesion; aromatic compounds; biosensors; diazonium compounds; industrial applications; nanoparticles; patents; polymerization; polymers; protein microarrays; salts
Abstract:
... This critical review summarizes existing knowledge on the use of diazonium salts as a new generation of surface modifiers and coupling agents for binding synthetic polymers, biomacromolecules, and nanoparticles to surfaces. Polymer grafts can be directly grown at surfaces through the so-called grafting from approaches based on several polymerization methods but can also be pre-formed in solution a ...
DOI:
10.1039/c0cs00179a

13. Cellular heterogeneity and live cell arrays

Author:
Maureen A. Walling; Jason R. E. Shepard
Source:
Chemical Society reviews 2011 v.40 no.7 pp. 4049-4076
ISSN:
1460-4744
Subject:
DNA; clones; monitoring; precision medicine; protein microarrays; systems biology
Abstract:
... In the past decade, the tendency to move from a global, one-size-fits-all treatment philosophy to personalized medicine is based, in part, on the nuanced differences and sub-classifications of disease states. Our knowledge of these varied states stems from not only the ability to diagnose, classify, and perform experiments on cell populations as a whole, but also from new technologies that allow i ...
DOI:
10.1039/c0cs00212g

14. High Density Diffusion-Free Nanowell Arrays

Author:
Takulapalli Bharath R.; Qiu Ji; Magee D. Mitchell; Kahn Peter; Brunner Al; Barker Kristi; Means Steven; Miersch Shane; Bian Xiaofang; Mendoza Alex; Festa Fernanda; Syal Karan; Park Jin G.; LaBaer Joshua; Wiktor Peter
Source:
Journal of Proteome Research 2012 v.11 no.8 pp. 4382-4391
ISSN:
1535-3907
Subject:
microarray technology; nucleic acids; protein synthesis; protein-protein interactions; proteins; proteome; proteomics; silicon
Abstract:
... Proteomics aspires to elucidate the functions of all proteins. Protein microarrays provide an important step by enabling high-throughput studies of displayed proteins. However, many functional assays of proteins include untethered intermediates or products, which could frustrate the use of planar arrays at very high densities because of diffusion to neighboring features. The nucleic acid programma ...
DOI:
10.1021/pr300467q
PubMed:
22742968
PubMed Central:
PMC3412896

15. Microfluidic Western blotting

Author:
Alex J. Hughes; Amy E. Herr
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.52 pp. 21450-21455
ISSN:
0027-8424
Subject:
Human immunodeficiency virus; Western blotting; antibodies; blue light; detection limit; gels; glass; humans; hydrophilicity; polyacrylamide; proteomics; transcription factor NF-kappa B; ultraviolet radiation
Abstract:
... Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ antibody probing. To validate the microfluidic as ...
DOI:
10.1073/pnas.1207754110
PubMed:
23223527
PubMed Central:
PMC3535594

16. Multifunctional Protein-Enabled Patterning on Arrayed Ferroelectric Materials

Author:
Hnilova M.; Liu X.; Yuca E.; Jia C.; Wilson B.; Karatas A. Y.; Gresswell C.; Ohuchi F.; Kitamura K.; Tamerler C.
Source:
ACS Applied Materials & Interfaces 2012 v.4 no.4 pp. 1865-1871
ISSN:
1944-8252
Subject:
geometry; green fluorescent protein; models; nanosilver; photochemistry
Abstract:
... This study demonstrates a biological route to programming well-defined protein-inorganic interfaces with an arrayed geometry via modular peptide tag technology. To illustrate this concept, we designed a model multifunctional fusion protein, which simultaneously displays a maltose-binding protein (MBP), a green fluorescence protein (GFPuv) and an inorganic-binding peptide (AgBP2C). The fused combin ...
DOI:
10.1021/am300177t

17. High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors

Author:
Dylan W. Domaille; Ju Hun Lee; Jennifer N. Cha
Source:
Chemical communications 2013 v.49 no.17 pp. 1759-1761
ISSN:
1364-548X
Subject:
DNA; bacteriophages; biosensors; catalytic activity; chemical communication; chemical reactions; colorimetry; fluorescent proteins; hydrazones; oligonucleotides; protein microarrays
Abstract:
... We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage–DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild co ...
DOI:
10.1039/c3cc38871a

18. Nanomaterials and biomaterials in electrochemical arrays for protein detection

Author:
James F. Rusling; Gregory W. Bishop; Nhi M. Doan; Fotios Papadimitrakopoulos
Source:
Journal of materials chemistry B 2013 v.2 no.1 pp. 12-30
ISSN:
2050-7518
Subject:
antibodies; biocompatible materials; diagnostic techniques; electrochemistry; heart diseases; immunoassays; liquids; magnetism; microfluidic technology; microparticles; nanoparticles; neoplasms; oligonucleotides; prediction; protein microarrays; proteins
Abstract:
... Nanomaterials and biomaterials are important components of new electrochemical arrays designed for sensitive detection of proteins in biological fluids. Such multiplexed protein arrays are predicted to have an important future in personalized medical diagnostics, especially for cancer and heart disease. Sandwich immunoassays for proteins benefit greatly in sensitivity from the use of nanostructure ...
DOI:
10.1039/c3tb21323d
PubMed:
24392222
PubMed Central:
PMC3878175

19. Non-specific clustering of histidine tagged green fluorescent protein mediated by surface interactions: the collective effect in the protein-adsorption behaviour

Author:
Tomasz D. Sobieściak; Piotr Zielenkiewicz
Source:
RSC advances 2013 v.3 no.26 pp. 10479-10486
ISSN:
2046-2069
Subject:
cell membranes; cost effectiveness; green fluorescent protein; histidine; metal ions; protein microarrays; topography; wettability
Abstract:
... The chemically programmed non-fouling surfaces were used to observe the surface assembly behaviour of hexahistidine tagged Green Fluorescent Protein (His-GFP) as a model protein. In this particular case, site-selective physisorption of His-GFP was achieved in the absence of metal ions. This preference does not arise from surface charge, wettability or topographic differences between regions. We fo ...
DOI:
10.1039/c3ra42154f

20. Autoantibody Signature for the Serologic Detection of Ovarian Cancer

Author:
Anderson Karen S.; Cramer Daniel W.; Sibani Sahar; Wallstrom Garrick; Wong Jessica; Park Jin; Qiu Ji; Vitonis Allison; LaBaer Joshua
Source:
Journal of Proteome Research 2015 v.14 no.1 pp. 578-586
ISSN:
1535-3907
Subject:
autoantibodies; autoantigens; biomarkers; immunoglobulin G; microarray technology; ovarian neoplasms; patients; proteome
Abstract:
... Sera from patients with ovarian cancer contain autoantibodies (AAb) to tumor-derived proteins that are potential biomarkers for early detection. To detect AAb, we probed high-density programmable protein microarrays (NAPPA) expressing 5177 candidate tumor antigens with sera from patients with serous ovarian cancer (n = 34 cases/30 controls) and measured bound IgG. Of these, 741 antigens were selec ...
DOI:
10.1021/pr500908n
PubMed:
25365139
PubMed Central:
PMC4334299

21. Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips

Author:
Manfè Valentina; Fleckner Jan; Nørby Lisby Peder; Zhang Bo; Dai Hongjie; Keller Pernille
Source:
BMC biotechnology 2015 v.15 no.1 pp. 73
ISSN:
1472-6750
Subject:
antibodies; blood serum; cytokines; detection limit; enzyme-linked immunosorbent assay; fluorescence; gold; immunoglobulin G; osteoarthritis; patient care; patients; protein content; protein microarrays; rheumatoid arthritis; synovial fluid
Abstract:
... BACKGROUND: Fluorescence-enhancing microarray on plasmonic gold film is an attractive alternative to traditional enzyme-linked immunosorbent assay (ELISA) for cytokine detection because of the increased sensitivity. The assay chemistry is similar to an ELISA sandwich assay, but owing to the gold substrate, cytokine measurements are 10 to 100 times more sensitive and can be multiplexed. Plasmonic p ...
DOI:
10.1186/s12896-015-0186-0
PubMed:
26268325
PubMed Central:
PMC4535377

22. Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

Author:
H. Yoshikawa; M. Murahashi; M. Saito; S. Jiang; M. Iga; E. Tamiya
Source:
Analytical methods 2015 v.7 no.12 pp. 5157-5161
ISSN:
1759-9679
Subject:
C-reactive protein; absorption; antibodies; antigen-antibody reactions; antigens; films (materials); humans; image analysis; immunoglobulin A; polymers; protein microarrays; spectral analysis; surface plasmon resonance
Abstract:
... We describe parallelized label-free detection of protein interactions using a hyper-spectral imaging system. Multi-array protein chips were fabricated by immobilizing antibodies on Au-capped nanopillar polymer films, which showed an optical absorption band due to the localised surface plasmon resonance (LSPR) in the absorption spectrum. Parallelized detection of multiple antigen–antibody interacti ...
DOI:
10.1039/c5ay00738k

23. Potent organo-osmium compound shifts metabolism in epithelial ovarian cancer cells

Author:
Jessica M. Hearn; Isolda Romero-Canelón; Alison F. Munro; Ying Fu; Ana M. Pizarro; Mathew J. Garnett; Ultan McDermott; Neil O. Carragher; Peter J. Sadler
Source:
Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.29 pp. E3800
ISSN:
0027-8424
Subject:
DNA; NADH dehydrogenase (ubiquinone); animal ovaries; cisplatin; cross resistance; epithelium; humans; mechanism of action; metabolism; mutation; neoplasm cells; osmium; ovarian neoplasms; reactive oxygen species
Abstract:
... The organometallic “half-sandwich” compound [Os(η ⁶- p -cymene)(4-(2-pyridylazo)- N,N -dimethylaniline)I]PF ₆ is 49× more potent than the clinical drug cisplatin in the 809 cancer cell lines that we screened and is a candidate drug for cancer therapy. We investigate the mechanism of action of compound 1 in A2780 epithelial ovarian cancer cells. Whole-transcriptome sequencing identified three misse ...
DOI:
10.1073/pnas.1500925112
PubMed:
26162681
PubMed Central:
PMC4517206

24. S-layer based biomolecular imprinting

Author:
Eva M. Ladenhauf; Dietmar Pum; Daniel S. Wastl; Jose Luis Toca-Herrera; Nam V. H. Phan; Peter A. Lieberzeit; Uwe B. Sleytr
Source:
RSC advances 2015 v.5 no.102 pp. 83558-83564
ISSN:
2046-2069
Subject:
Archaea; antibodies; bacteria; biopolymers; ferritin; films (materials); gels; moieties; molecular imprinting; periodicity; protein microarrays; quartz crystal microbalance; silicon
Abstract:
... This work describes the development of molecularly imprinted polymer (MIP) thin films by using reassembled S-layer protein arrays as templates. Crystalline bacterial cell surface layer (S-layer) proteins are among the most abundant biopolymers on earth and form the outermost cell envelope component in a broad range of bacteria and archaea. The unique feature of S-layer based imprints is the crysta ...
DOI:
10.1039/c5ra14971a

25. Detection, quantification, and profiling of PSA: current microarray technologies and future directions

Author:
Mukesh Digambar Sonawane; Satish Balasaheb Nimse; Keum-Soo Song; Taisun Kim
Source:
RSC advances 2016 v.6 no.9 pp. 7599-7609
ISSN:
2046-2069
Subject:
DNA microarrays; biomarkers; blood; death; men; monitoring; mortality; prostate gland; prostatic neoplasms; protein microarrays; therapeutics
Abstract:
... Prostate cancer (PCa) is a cancer of the prostate gland. The death rate of 13% among the men diagnosed with PCa makes it the second leading cause of cancer death. It has been reported that the monitoring of the progression of PCa and response to therapy is done by measuring the level of blood PSA. Even though PSA has been used for the diagnosis of PCa, the current scenario dictates the necessity o ...
DOI:
10.1039/c5ra20313a

26. High throughput platform to explore RNA–protein interactomes

Author:
F. X. Reymond Sutandy; Felix Shih-Hsiang Hsiao; Chien-Sheng Chen
Source:
Critical reviews in biotechnology 2016 v.36 no.1 pp. 11-19
ISSN:
1549-7801
Subject:
RNA; RNA-binding proteins; molecular biology; protein microarrays
Abstract:
... RNA binding proteins (RBPs) and RNA interaction is an emerging topic in molecular biology. Many reports showed that such interactions contribute to many cellular processes as well as disease development. Several standard in vitro and in vivo methods were developed to fulfill the needs of this RBP–RNA interaction study to explore their biological functions. However, these methods have their limitat ...
DOI:
10.3109/07388551.2014.922916

27. PAA: an R/bioconductor package for biomarker discovery with protein microarrays

Author:
Michael Turewicz; Maike Ahrens; Caroline May; Katrin Marcus; Martin Eisenacher
Source:
Bioinformatics 2016 v.32 no.10 pp. 1577-1579
ISSN:
1460-2059
Subject:
bioinformatics; biomarkers; protein microarrays; quality control; selection methods
Abstract:
... Summary: The R/Bioconductor package Protein Array Analyzer (PAA) facilitates a flexible analysis of protein microarrays for biomarker discovery (esp., ProtoArrays). It provides a complete data analysis workflow including preprocessing and quality control, uni- and multivariate feature selection as well as several different plots and results tables to outline and evaluate the analysis results. As a ...
DOI:
10.1093/bioinformatics/btw037
PubMed:
26803161
PubMed Central:
PMC4866526

28. Precise protein assembly of array structures

Author:
Guang Yang; Libin Wu; Guosong Chen; Ming Jiang
Source:
Chemical communications 2016 v.52 no.70 pp. 10595-10605
ISSN:
1364-548X
Subject:
chemical compounds; chemical reactions; electrostatic interactions; microstructure; protein microarrays; protein-protein interactions; proteins
Abstract:
... The assembly of proteins into various nano-objects with regular and periodic microstructures, i.e. protein arrays, is a fast-growing field in materials science. Due to the structural complexity of proteins, reports in this field are still quite limited. In this review, we summarize the recent developments in protein array construction by different driving forces, including electrostatic interactio ...
DOI:
10.1039/c6cc04190f

29. An ultrasensitive colorimetric strategy for protein O-GlcNAcylation detection via copper deposition-enabled nonenzymatic signal amplification

Author:
Yingshuai Liu; Jin Xie; Zeying Zhang; Zhisong Lu
Source:
RSC advances 2016 v.6 no.92 pp. 89484-89491
ISSN:
2046-2069
Subject:
Alzheimer disease; absorbance; agglutinins; catalytic activity; cell lines; colorimetry; coordination compounds; copper; copper nanoparticles; detection limit; diabetes; ferric chloride; human diseases; iron; lectins; models; nanogold; neoplasm cells; post-translational modification; prostatic neoplasms; protein microarrays; quantitative analysis; redox reactions; ultraviolet-visible spectroscopy; wheat germ
Abstract:
... O-GlcNAcylation is a very important post-translational modification of nucleocytoplasmic proteins. Aberrant O-GlcNacylation has been found in a variety of human diseases including many types of cancers, diabetes, and Alzheimer's disease. However, analysis of protein O-GlcNacylation still remains a great challenge due to its low levels and labile nature. Herein, a highly sensitive and reliable colo ...
DOI:
10.1039/c6ra17119b

30. Analysis of changes in the glycan composition of serum, cytosol and membrane glycoprotein biomarkers of colorectal cancer using a lectin-based protein microarray

Author:
Martina Zámorová; Alena Holazová; Goran Miljuš; Dragana Robajac; Miloš Šunderić; Vesna Malenković; Blagoje Đukanović; Peter Gemeiner; Jaroslav Katrlík; Olgica Nedić
Source:
Analytical methods 2017 v.9 no.18 pp. 2660-2666
ISSN:
1759-9679
Subject:
Aleuria; Maackia amurensis; Sambucus nigra; analytical methods; biomarkers; blood serum; cell membranes; colorectal neoplasms; cytosol; fluorescence; glycoproteins; glycosylation; insulin-like growth factor binding proteins; lectins; membrane glycoproteins; monitoring; neoplasm cells; patients; protein microarrays; scanners; streptavidin
Abstract:
... The altered glycosylation of proteins is a cancer-related marker and the monitoring of glycosylation status can significantly increase the informative value of protein biomarkers. Herein is presented an analytical method for the investigation of the glycan composition of colorectal cancer (CRC) protein biomarkers applied to different sample types using a lectin-based microarray platform. The sampl ...
DOI:
10.1039/c7ay00159b

31. Plasmonic nanograting enhanced fluorescence for protein microarray analysis of carcinoembryonic antigen (CEA)

Author:
Chang Liu; Fanling Meng; Baogang Wang; Lei Zhang; Xiaoqiang Cui
Source:
Analytical methods 2017 v.10 no.1 pp. 145-150
ISSN:
1759-9679
Subject:
analytical methods; antigens; biomarkers; detection limit; diagnostic techniques; fluorescence; glass; gold; nanogold; neoplasms; protein microarrays; proteomics
Abstract:
... The low sensitivity of protein microarrays has limited the wide application in diagnostics and high-throughput proteomic analysis. We present, for the first time, the exploration of protein microarray analysis on nanostructured plasmonic gold nanograting that showed a 10-fold fluorescence enhancement. The as-fabricated plasmonic protein microarray showed a limit of detection (LOD) of 0.36 ng mL⁻¹ ...
DOI:
10.1039/c7ay02232h

32. Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins

Author:
Janine Schulte-Zweckel; Tabea Schneidewind; Jose Luis Abad; Andreas Brockmeyer; Petra Janning; Gemma Triola
Source:
Chemical communications 2018 v.54 no.97 pp. 13742-13745
ISSN:
1364-548X
Subject:
apoptosis; autophagy; ceramides; chemical reactions; inflammation; mass spectrometry; protein microarrays; proteins; proteomics
Abstract:
... Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profilin ...
DOI:
10.1039/c8cc05691a

33. DNA-Corralled Nanodiscs for the Structural and Functional Characterization of Membrane Proteins and Viral Entry

Author:
Zhao Zhao; Meng Zhang; James M. Hogle; William M. Shih; Gerhard Wagner; Mahmoud L. Nasr
Source:
Journal of the American Chemical Society 2018 v.140 no.34 pp. 10639-10643
ISSN:
1520-5126
Subject:
DNA; Enterovirus C; lipids; manufacturing; membrane proteins; models; protein microarrays
Abstract:
... Here we present a modular method for manufacturing large-sized nanodiscs using DNA-origami barrels as scaffolding corrals. Large-sized nanodiscs can be produced by first decorating the inside of DNA barrels with small lipid-bilayer nanodiscs, which open up when adding extra lipid to form large nanodiscs of diameters ∼45 or ∼70 nm as prescribed by the enclosing barrel dimension. Densely packed memb ...
DOI:
10.1021/jacs.8b04638
PubMed:
30094995
PubMed Central:
PMC6206850

34. Effect of nutritional supplement on bone marrow-derived mesenchymal stem cells from aplastic anaemia

Author:
Shihua Luo; Yinghai Chen; Lifen Zhao; Xia Qi; Xiaoyan Miao; Huimin Zhou; Li Jia
Source:
British journal of nutrition 2018 v.119 no.7 pp. 748-758
ISSN:
1475-2662
Subject:
X-radiation; adipocytes; amino acids; anemia; animal models; bioactive properties; blood composition; blood serum; bone density; bone formation; bone marrow; chloramphenicol; cyclophosphamide; dietary supplements; dose response; gene expression regulation; hematopoiesis; hematopoietic stem cells; intragastric administration; mesenchymal stromal cells; nucleotides; nutritional support; protein microarrays; protein synthesis; rats; vitamins
Abstract:
... Aplastic anaemia (AA) is characterised by pancytopenia resulting from a marked reduction in haemopoietic stem cells (HSC). The regulation of haemopoiesis depends on the interaction between HSC and various cells of the bone marrow (BM) microenvironment, including BM-derived mesenchymal stromal cells (BMSC). The purpose of this study was to analyse the biological effect of nutritional supplement (NS ...
DOI:
10.1017/S0007114518000399

35. Fluorescence-based kinetic analysis of miniaturized protein microarrays

Author:
Heman Kumar Ramiya Ramesh Babu; Levi A. Gheber
Source:
Biosensors & bioelectronics 2018 v.122 pp. 290-299
ISSN:
0956-5663
Subject:
biosensors; fluorescence; kinetics; microfluidic technology; monitoring; protein microarrays
Abstract:
... Ideal monitoring devices should enjoy a combination of characteristics, e.g. high sensitivity, multiplexing, portability, short time-to-result (TTR). Typically, no device meets all of these demands since some of them are contradictory, to some extent. Herein, we present a miniaturized platform based on fluorescent detection, which is sensitive, readily allows multiplexing, and allows real-time mon ...
DOI:
10.1016/j.bios.2018.09.051

36. Genetic conservation of Cytauxzoon felis antigens and mRNA expression in the schizont life-stage

Author:
Daven B. Khana; David S. Peterson; James B. Stanton; Megan E. Schreeg; Adam J. Birkenheuer; Jaime L. Tarigo
Source:
Veterinary parasitology 2018 v.263 pp. 49-53
ISSN:
0304-4017
Subject:
Cytauxzoon felis; Protozoa; Theileria; animal tissues; antigens; cat diseases; cats; gene expression; genome; germplasm conservation; immune response; in situ hybridization; messenger RNA; morbidity; mortality; parasites; protein microarrays; sequence analysis; vaccine development; vaccines
Abstract:
... Cytauxzoonosis is a highly fatal disease of domestic cats caused by the apicomplexan protozoan Cytauxzoon felis, which is most closely related to Theileria spp. The growing prevalence, high morbidity and mortality, and treatment cost of cytauxzoonosis emphasize the need for vaccine development. Traditional approaches for vaccine development, however, have been hindered by the inability to culture ...
DOI:
10.1016/j.vetpar.2018.10.007

37. The Immunome of Colon Cancer: Functional In Silico Analysis of Antigenic Proteins Deduced from IgG Microarray Profiling

Author:
Johana A. Luna Coronell; Khulan Sergelen; Philipp Hofer; István Gyurján; Stefanie Brezina; Peter Hettegger; Gernot Leeb; Karl Mach; Andrea Gsur; Andreas Weinhäusel
Source:
Genomics, proteomics & bioinformatics 2018 v.16 no.1 pp. 73-84
ISSN:
1672-0229
Subject:
antigens; autoantibodies; bioinformatics; cell death; colorectal neoplasms; computer simulation; databases; genomics; humans; protein microarrays; proteomics
Abstract:
... Characterization of the colon cancer immunome and its autoantibody signature from differentially-reactive antigens (DIRAGs) could provide insights into aberrant cellular mechanisms or enriched networks associated with diseases. The purpose of this study was to characterize the antibody profile of plasma samples from 32 colorectal cancer (CRC) patients and 32 controls using proteins isolated from 1 ...
DOI:
10.1016/j.gpb.2017.10.002

38. Induction of nerve growth factor by phorbol 12-myristate 13-acetate is dependent upon the mitogen activated protein kinase pathway

Author:
Justin B. Davis; Valerie Calvert; Steven Roberts; Sabrina Bracero; Emanuel Petricoin; Robin Couch
Source:
Heliyon 2018 v.4 no.5 pp. e00617
ISSN:
2405-8440
Subject:
Alzheimer disease; agonists; apoptosis; cell proliferation; chemoprevention; exocytosis; gene expression; humans; mitogen-activated protein kinase; nerve growth factor; neurons; protein kinase C; protein microarrays; protein phosphorylation; secretion; signal transduction; therapeutics
Abstract:
... Several small molecules have been identified that induce glial cells to synthesize and secrete nerve growth factor (NGF), a critical neurotrophin that supports neuronal growth and survival, and as such show promise in the development of drugs for the chemoprevention of Alzheimer's disease. To map the signal transduction cascade leading to NGF synthesis and secretion, cultured human glial cells wer ...
DOI:
10.1016/j.heliyon.2018.e00617
PubMed:
29872754
PubMed Central:
PMC5986306

39. Novel protein chip for the detection of antibodies against infectious bronchitis virus

Author:
Liping Yan; Jianhua Hu; Jing Lei; Zhiyu Shi; Qian Xiao; Zhenwei Bi; Lu Yao; Yuan Li; Yuqing Chen; An Fang; Hui Li; Suquan Song; Min Liao; Jiyong Zhou
Source:
BMC veterinary research 2018 v.14 no.1 pp. 284
ISSN:
1746-6148
Subject:
diagnostic techniques; Avian orthoavulavirus 1; analytical kits; Infectious bursal disease virus; vaccination; Chicken anemia virus; cross reaction; chemiluminescence; viral nonstructural proteins; antibodies; veterinary medicine; enzyme-linked immunosorbent assay; viruses; pathogens; Escherichia coli; monitoring; infectious bronchitis; Infectious bronchitis virus; chickens; Influenza A virus; Mardivirus; protein microarrays
Abstract:
... BACKGROUND: Infectious bronchitis (IB) caused by the IB virus (IBV) can cause acute damage to chickens around the world. Therefore, rapid diagnosis and immune status determination are critical for controlling IBV outbreaks. Enzyme-linked immunosorbent assays (ELISAs) have been widely used in the detection of IBV antibodies in the early infection and continuous infection of IB because they are more ...
DOI:
10.1186/s12917-018-1586-x
PubMed:
30223836
PubMed Central:
PMC6142349

40. Peanut allergens

Author:
Chiara Palladino; Heimo Breiteneder
Source:
Molecular immunology 2018 v.100 pp. 58-70
ISSN:
0161-5890
Subject:
Arachis hypogaea; algae; allergens; antibodies; clinical trials; immunoglobulin E; immunotherapy; insects; peanuts; protein microarrays; protocols; quality of life; risk; seeds; yeasts
Abstract:
... Peanut allergens have the potential to negatively impact on the health and quality of life of millions of consumers worldwide. The seeds of the peanut plant Arachis hypogaea contain an array of allergens that are able to induce the production of specific IgE antibodies in predisposed individuals. A lot of effort has been focused on obtaining the sequences and structures of these allergens due to t ...
DOI:
10.1016/j.molimm.2018.04.005
PubMed:
29680589
PubMed Central:
PMC7060077

41. Rapid detection of four mycotoxins in corn using a microfluidics and microarray-based immunoassay system

Author:
Yiqi Chen; Xiangrui Meng; Yunzeng Zhu; Minjie Shen; Ying Lu; Jing Cheng; Youchun Xu
Source:
Talanta 2018 v.186 pp. 299-305
ISSN:
0039-9140
Subject:
T-2 toxin; aflatoxin B1; corn; detection limit; forage; human health; image analysis; immunoassays; microfluidic technology; ochratoxin A; protein microarrays; prototypes; rapid methods; zearalenone
Abstract:
... Mycotoxins threaten human health seriously because they usually exist in food, fodder and commodities. In this study, a rapid and sensitive immunoassay system for commonly encountered mycotoxins was established based on microfluidics and protein microarrays. Four mycotoxins (T-2 toxin, aflatoxin B1, ochratoxin A, and zearalenone) can be automatically detected in a custom-made microdevice within 30 ...
DOI:
10.1016/j.talanta.2018.04.064

42. Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses

Author:
Mohammed Gagaoua; Muriel Bonnet; Marie-Pierre Ellies-Oury; Leanne De Koning; Brigitte Picard
Source:
Food chemistry 2018 v.250 pp. 245-252
ISSN:
0308-8146
Subject:
Charolais; apoptosis; beef; biomarkers; cattle; cytoskeleton; energy metabolism; enzymes; heat stress; longissimus muscle; myosin heavy chains; oxidative stress; prediction; protein microarrays; tenderizing; texture
Abstract:
... The validation of biomarkers and tools for the prediction of beef texture remains a challenging task. In this study, reverse phase protein arrays (RPPA) quantified 29 protein biomarkers in the m. Longissimus thoracis of Charolais cattle sampled early post-mortem. Myosin heavy chain 1 (MHC1, slow-oxidative fibers) and Retinal dehydrogenase 1 (ALDH1A1, oxidative enzyme) discriminated between tender ...
DOI:
10.1016/j.foodchem.2018.01.070

43. Single step, direct fluorescence immunoassays based on metal enhanced fluorescence (MEF-FIA) applicable as micro plate-, array-, multiplexing- or point of care-format

Author:
G. Hawa; Linda Sonnleitner; A. Missbichler; A. Prinz; G. Bauer; C. Mauracher
Source:
Analytical biochemistry 2018 v.549 pp. 39-44
ISSN:
0003-2697
Subject:
antibodies; assays; biomarkers; biomedical research; chemical species; detection; diagnostic techniques; enzyme substrates; enzymes; equipment; fluorescence; fluorescent antibody technique; formates; laboratories; point-of-care systems; protein microarrays; proteins; sorbents; washing
Abstract:
... Although Enzyme Linked Immuno Sorbent Assay (ELISA) technology is approaching it's 45th year of existence since first described in 1971, it is still the main diagnostic tool in clinical research and routine diagnostics.However, despite its broad usage it suffers from some drawbacks, limiting its use especially in more advanced assay formats like multiplexing platforms, point of care devices or pro ...
DOI:
10.1016/j.ab.2018.03.002

44. Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray

Author:
Andrew C. Petersen; Jeannett M. Clampitt; F. Chris Minion
Source:
Veterinary microbiology 2019 pp. -
ISSN:
0378-1135
Subject:
Escherichia coli; Mycoplasma hyopneumoniae; adhesins; antibodies; antigen-antibody reactions; codons; enzyme-linked immunosorbent assay; mycoplasmosis; polymerase chain reaction; protein microarrays; serology; surface proteins; swine
Abstract:
... Pigs harbor several different species of mycoplasmas, of which Mycoplasma hyopneumoniae presents the most significant economic impact on the swine industry. While ELISAs are the predominant diagnostic assay to measure antibody responses during infection with M. hyopneumoniae, the assay itself is only a rough estimate of the total antibody response. It lends little information on pathogen-wide anti ...
DOI:
10.1016/j.vetmic.2019.02.010

45. Aspartate Residues Far from the Active Site Drive O-GlcNAc Transferase Substrate Selection

Author:
Cassandra M. Joiner; Zebulon G. Levine; Chanat Aonbangkhen; Christina M. Woo; Suzanne Walker
Source:
Journal of the American Chemical Society 2019 v.141 no.33 pp. 12974-12978
ISSN:
1520-5126
Subject:
Animalia; active sites; aspartic acid; enzyme substrates; glycosylation; models; neoplasm progression; neoplasms; protein microarrays; proteins; transferases
Abstract:
... O-GlcNAc is an abundant post-translational modification found on nuclear and cytoplasmic proteins in all metazoans. This modification regulates a wide variety of cellular processes, and elevated O-GlcNAc levels have been implicated in cancer progression. A single essential enzyme, O-GlcNAc transferase (OGT), is responsible for all nucleocytoplasmic O-GlcNAcylation. Understanding how this enzyme ch ...
DOI:
10.1021/jacs.9b06061
PubMed:
31373491
PubMed Central:
PMC6849375

46. Assessing Anticancer Potential of Blueberry Flavonoids, Quercetin, Kaempferol, and Gentisic Acid, Through Oxidative Stress and Apoptosis Parameters on HCT-116 Cells

Author:
Ebru Demirel Sezer; Latife Merve Oktay; Elif Karadadaş; Hikmet Memmedov; Nur Selvi Gunel; Eser Sözmen
Source:
Journal of medicinal food 2019 v.22 no.11 pp. 1118-1126
ISSN:
1557-7600
Subject:
2,5-dihydroxybenzoic acid; Vaccinium myrtillus; antineoplastic activity; antineoplastic agents; antioxidant activity; antioxidants; apoptosis; blueberries; cytotoxicity; diet; flow cytometry; fruit extracts; kaempferol; liquid chromatography; neoplasms; oxidants; oxidative stress; polyphenols; protein composition; protein microarrays; proteins; quercetin; tandem mass spectrometry; therapeutics
Abstract:
... In recent years, natural products gained popularity with their anti-inflammatory and antioxidant effects mediated by chemical compounds within their composition. Study results offering them as palliative therapy options in cancer or as anticancer agents with high levels of cytotoxicity brought a new approach to combine cancer treatment protocols with these products. From a different perspective, e ...
DOI:
10.1089/jmf.2019.0098

47. Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19)

Author:
Elvira Gonzalez de Mejia; Candice Mazewski; Diego Luna-Vital; Mark Berhow
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
acclimation; animal models; anthocyanins; anti-inflammatory activity; azoxymethane; body weight; carcinogenesis; colon; colorectal neoplasms; delphinidin; dextran; disease control; enzyme-linked immunosorbent assay; freeze drying; gallic acid; gene expression; histology; immune response; inflammation; lentils; mice; neoplasm cells; placebos; polyphenols; protein microarrays; sodium sulfate; spleen
Abstract:
... The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model. BL was obtained from an overnight soak of organic beluga black lentils (1:2 w/v) in water, filtered, frozen, and freeze- ...
DOI:
10.1093/cdn/nzz030.OR04-06-19

48. Development and validation of different indirect ELISAs for MERS-CoV serological testing

Author:
Anwar M. Hashem; Sawsan S. Al-amri; Tagreed L. Al-subhi; Loai A. Siddiq; Ahmed M. Hassan; Maha M. Alawi; Rowa Y. Alhabbab; Salwa I. Hindawi; Osama B. Mohammed; Nabil S. Amor; Abdulaziz N. Alagaili; Ahmed A. Mirza; Esam I. Azhar
Source:
Journal of immunological methods 2019 v.466 pp. 41-46
ISSN:
0022-1759
Subject:
Middle East respiratory syndrome coronavirus; amino acids; blood serum; enzyme-linked immunosorbent assay; equipment; fluorescent antibody technique; human resources; humans; monitoring; mortality; neutralization; neutralization tests; nucleocapsid proteins; protein microarrays; seroprevalence; Saudi Arabia
Abstract:
... Since 2012, MERS-CoV has caused up to 2220 cases and 790 deaths in 27 countries with Saudi Arabia being the most affected country with ~83.1% of the cases and ~38.8% local death rate. Current serological assays such as microneutralization (MN), plaque reduction neutralization, immunofluorescence, protein microarray or pseudoparticle neutralization assays rely on handling of live MERS-CoV in high c ...
DOI:
10.1016/j.jim.2019.01.005
PubMed:
30659836
PubMed Central:
PMC7094657

49. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

Author:
Samuel White; Meriel Moore‐Colyer; Eliane Marti; Laurent Coüetil; Duncan Hannant; Eric A. Richard; Marcos Alcocer
Source:
Journal of veterinary internal medicine 2019 v.33 no.5 pp. 2327-2335
ISSN:
0891-6640
Subject:
allergens; arthropods; asthma; bacteria; filters; fungi; horses; immunoglobulin E; mice; pollen; protein microarrays
Abstract:
... BACKGROUND: Severe asthma in horses, known as severe equine asthma (SEA), is a prevalent, performance‐limiting disease associated with increased allergen‐specific immunoglobulin E (IgE) against a range of environmental aeroallergens. OBJECTIVE: To develop a protein microarray platform to profile IgE against a range of proven and novel environmental proteins in SEA‐affected horses. ANIMALS: Six SEA ...
DOI:
10.1111/jvim.15564
PubMed:
31429513
PubMed Central:
PMC6766494

50. Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax

Author:
Seong-Kyun Lee; Jin-Hee Han; Ji-Hoon Park; Kwon-Soo Ha; Won Sun Park; Seok-Ho Hong; Sunghun Na; Yang Cheng; Eun-Taek Han
Source:
Parasites & vectors 2019 v.12 no.1 pp. 594
ISSN:
1756-3305
Subject:
Plasmodium vivax; amino acids; bioinformatics; blood serum; cell free system; cell-free protein synthesis; computer software; erythrocytes; genes; germ cells; immunization; immunoglobulin G; malaria; membrane proteins; parasites; pathogens; patients; protein microarrays; protein structure; vaccine development; wheat germ
Abstract:
... BACKGROUND: Malaria parasites form intracellular membranes that separate the parasite from the internal space of erythrocytes, and membrane proteins from the parasites are exported to the host via the membrane. In our previous study, Plasmodium vivax early transcribed membrane protein (PvETRAMP) 11.2, an intracellular membrane protein that is highly expressed in blood-stage parasites, was characte ...
DOI:
10.1186/s13071-019-3846-4
PubMed:
31856917
PubMed Central:
PMC6921578