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You searched for: Subject term protein microarrays Remove constraint Subject term: protein microarrays
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1. A chemoenzymatic approach enables the site‐specific conjugation of recombinant proteins

Author:
Yan Wang; Mingming Dong; Mingliang Ye
Source:
Electrophoresis 2019 v.40 no.16-17 pp. 2125-2128
ISSN:
0173-0835
Subject:
aldehydes; biosensors; chemical bonding; drugs; electrophoresis; hydrazides; hydrazones; oxidation; protein microarrays; proteinases; recombinant proteins; screening; serine; synthetic peptides; tyrosine
Abstract:
... Many biotechniques including protein microarray, drug screening, biosensors rely on the immobilization of recombinant proteins on the solid supports. It is well known that random orientation of the immobilized proteins could impair their biologic functions. Thus, it is very important to develop new site‐specific immobilization approach. In this study, we presented a chemoenzymatic approach for sit ...
DOI:
10.1002/elps.201800528

2. A decade of Nucleic Acid Programmable Protein Arrays (NAPPA) availability: News, actors, progress, prospects and access

Author:
Raúl Manzano-Román; Manuel Fuentes
Source:
Journal of proteomics 2019 v.198 pp. 27-35
ISSN:
1874-3919
Subject:
cell physiology; molecular biology; nucleic acids; peptides; protein microarrays; protein-protein interactions; proteins; proteome; proteomics; therapeutics
Abstract:
... Understanding the dynamic of the proteome is a critical challenge because it requires high sensitive methodologies in high-throughput formats in order to decipher its modifications and complexity. While molecular biology provides relevant information about cell physiology that may be reflected in post-translational changes, High-Throughput (HT) experimental proteomic techniques are essential to pr ...
DOI:
10.1016/j.jprot.2018.12.007

3. A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

Author:
Yue Wu; Xudan Wu; Jing Chen; Jingfei Hu; Xiaobo Huang; Bin Zhou
Source:
BMC veterinary research 2020 v.16 no.1 pp. 162
ISSN:
1746-6148
Subject:
Betaarterivirus suid 1; Bluetongue virus; Classical swine fever virus; Infectious bronchitis virus; Japanese encephalitis virus; Ungulate protoparvovirus 1; Western blotting; antibodies; blood serum; cross reaction; detection; enzyme-linked immunosorbent assay; glass; mixed infection; pathogens; plasmids; protein microarrays; proteins; research; ruminants; sampling; swine; swine diseases; veterinary medicine; viruses; China
Abstract:
... BACKGROUND: At present, pig industry in China is faced with the complex situation of mixed infection caused by multiple pathogens. It is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies. Biochip array technology has made it possible to screen thousands of samples simultaneously; it has been twice named as one of the top 10 scien ...
DOI:
10.1186/s12917-020-02375-7

4. Activity-based fingerprinting and inhibitor discovery of cysteine proteases in a microarray

Author:
Mahesh Uttamchandani; Kai Liu; Resmi C. Panicker; Shao Q. Yao
Source:
Chemical communications 2007 v. no.15 pp. 1518-1520
ISSN:
1364-548X
Subject:
chemical compounds; chemical reactions; cysteine proteinases; gels; peptides; protein microarrays
Abstract:
... A panel of 20 peptide vinyl sulfone probes has been synthesized and used to generate activity-based fingerprinting profiles of cysteine proteases in both gel- and microarray-based formats; the inhibitor fingerprints of representative small molecule inhibitors targeted against 4 cysteine proteases were also obtained, in high-throughput, using the same protein microarray platform. ...
DOI:
10.1039/b702826a

5. Adenosylhomocysteinase like 1 interacts with nonstructural 5A and regulates hepatitis C virus propagation

Author:
Yun-Sook Lim; Han N. Mai; Lap P. Nguyen; Sang Min Kang; Dongseob Tark; Soon B. Hwang
Source:
journal of microbiology 2021 v.59 no.1 pp. 101-109
ISSN:
1225-8873
Subject:
Hepatitis C virus; adenosylhomocysteinase; humans; microbiology; mutants; precipitin tests; protein microarrays; protein synthesis; ribosomes
Abstract:
... Hepatitis C virus (HCV) life cycle is highly dependent on cellular proteins for viral propagation. In order to identify the cellular factors involved in HCV propagation, we previously performed a protein microarray assay using the HCV nonstructural 5A (NS5A) protein as a probe. Of ∼9,000 human cellular proteins immobilized in a microarray, adenosylhomocysteinase like 1 (AHCYL1) was among 90 protei ...
DOI:
10.1007/s12275-021-0470-8

6. Advanced glycation end products (AGEs) synergistically potentiated the proinflammatory action of lipopolysaccharide (LPS) and high mobility group box-1 (HMGB1) through their direct interactions

Author:
Masahiro Watanabe; Takao Toyomura; Mayuko Tomiyama; Hidenori Wake; Keyue Liu; Kiyoshi Teshigawara; Hideo Takahashi; Masahiro Nishibori; Shuji Mori
Source:
Molecular biology reports 2020 v.47 no.9 pp. 7153-7159
ISSN:
0301-4851
Subject:
Toll-like receptor 4; advanced glycation end products; apoptosis; gene expression; inflammation; lipopolysaccharides; molecular biology; nucleoproteins; protein microarrays
Abstract:
... Previously, we found that advanced glycation endproducts (AGEs) directly interact with tumor necrosis factor (TNF)-like weak inducer of apoptosis, a cytokine that controls inflammation, and that this interaction inhibited its action. This finding raised the novel possibility that AGEs alter the function of other cytokines through direct interaction. To investigate this possibility, we performed co ...
DOI:
10.1007/s11033-020-05783-y

7. Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort

Author:
Muneerah Smith; Houari B. Abdesselem; Michelle Mullins; Ti-Myen Tan; Andrew J. M. Nel; Maryam A. Y. Al-Nesf; Ilham Bensmail; Nour K. Majbour; Nishant N. Vaikath; Adviti Naik; Khalid Ouararhni; Vidya Mohamed-Ali; Mohammed Al-Maadheed; Darien T. Schell; Seanantha S. Baros-Steyl; Nur D. Anuar; Nur H. Ismail; Priscilla E. Morris; Raja N. R. Mamat; Nurul S. M. Rosli; Arif Anwar; Kavithambigai Ellan; Rozainanee M. Zain; Wendy A. Burgers; Elizabeth S. Mayne; Omar M. A. El-Agnaf; Jonathan M. Blackburn
Source:
Viruses 2021 v.13 no.5 pp. -
ISSN:
1999-4915
Subject:
COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; disease severity; epitopes; humoral immunity; immunologic techniques; lifestyle; nationalities and ethnic groups; nucleocapsid proteins; protein microarrays; seroprevalence; vaccines
Abstract:
... The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microa ...
DOI:
10.3390/v13050786

8. An Integrated Systems Biology Approach Identifies the Proteasome as A Critical Host Machinery for ZIKV and DENV Replication

Author:
Guang Song; Emily M. Lee; Jianbo Pan; Miao Xu; Hee-Sool Rho; Yichen Cheng; Nadia Whitt; Shu Yang; Jennifer Kouznetsova; Carleen Klumpp-Thomas; Samuel G. Michael; Cedric Moore; Ki-Jun Yoon; Kimberly M. Christian; Anton Simeonov; Wenwei Huang; Menghang Xia; Ruili Huang; Madhu Lal-Nag; Hengli Tang; Wei Zheng; Jiang Qian; Hongjun Song; Guo-li Ming; Heng Zhu
Source:
Genomics, proteomics & bioinformatics 2021 v.19 no.1 pp. 108-122
ISSN:
1672-0229
Subject:
Dengue virus; Zika virus; data collection; drugs; genomics; humans; multiomics; pathogenesis; proteasome endopeptidase complex; protein microarrays; protein-protein interactions; proteomics; virus replication
Abstract:
... The Zika virus (ZIKV) and dengue virus (DENV) flaviviruses exhibit similar replicative processes but have distinct clinical outcomes. A systematic understanding of virus–host protein–protein interaction networks can reveal cellular pathways critical to viral replication and disease pathogenesis. Here we employed three independent systems biology approaches toward this goal. First, protein array an ...
DOI:
10.1016/j.gpb.2020.06.016

9. An integrated micro-electro-fluidic and protein arraying system for parallel analysis of cell responses to controlled microenvironments

Author:
Zhizhong Yin; Sheng-Ce Tao; Raymond Cheong; Heng Zhu; Andre Levchenko
Source:
Integrative biology 2010 v.2 no.9 pp. 416-423
ISSN:
1757-9708
Subject:
cellular microenvironment; dielectrophoresis; electrodes; endothelial cells; extracellular matrix; glass; ligands; liquids; polydimethylsiloxane; protein microarrays; transcription factor NF-kappa B; tumor necrosis factors
Abstract:
... Living cells have evolved sophisticated signaling networks allowing them to respond to a wide array of external stimuli. Microfluidic devices, facilitating the analysis of signaling networks through precise definition of the cellular microenvironment often lack the capacity of delivering multiple combinations of different signaling cues, thus limiting the throughput of the analysis. To address thi ...
DOI:
10.1039/c0ib00017e
PubMed:
20714638
PubMed Central:
PMC3902009

10. An ultrasensitive colorimetric strategy for protein O-GlcNAcylation detection via copper deposition-enabled nonenzymatic signal amplification

Author:
Yingshuai Liu; Jin Xie; Zeying Zhang; Zhisong Lu
Source:
RSC advances 2016 v.6 no.92 pp. 89484-89491
ISSN:
2046-2069
Subject:
Alzheimer disease; absorbance; agglutinins; catalytic activity; cell lines; colorimetry; coordination compounds; copper; copper nanoparticles; detection limit; diabetes; ferric chloride; human diseases; iron; lectins; models; nanogold; neoplasm cells; post-translational modification; prostatic neoplasms; protein microarrays; quantitative analysis; redox reactions; ultraviolet-visible spectroscopy; wheat germ
Abstract:
... O-GlcNAcylation is a very important post-translational modification of nucleocytoplasmic proteins. Aberrant O-GlcNacylation has been found in a variety of human diseases including many types of cancers, diabetes, and Alzheimer's disease. However, analysis of protein O-GlcNacylation still remains a great challenge due to its low levels and labile nature. Herein, a highly sensitive and reliable colo ...
DOI:
10.1039/c6ra17119b

11. Analysis of changes in the glycan composition of serum, cytosol and membrane glycoprotein biomarkers of colorectal cancer using a lectin-based protein microarray

Author:
Martina Zámorová; Alena Holazová; Goran Miljuš; Dragana Robajac; Miloš Šunderić; Vesna Malenković; Blagoje Đukanović; Peter Gemeiner; Jaroslav Katrlík; Olgica Nedić
Source:
Analytical methods 2017 v.9 no.18 pp. 2660-2666
ISSN:
1759-9679
Subject:
Aleuria; Maackia amurensis; Sambucus nigra; analytical methods; biomarkers; blood serum; cell membranes; colorectal neoplasms; cytosol; fluorescence; glycoproteins; glycosylation; insulin-like growth factor binding proteins; lectins; membrane glycoproteins; monitoring; neoplasm cells; patients; protein microarrays; scanners; streptavidin
Abstract:
... The altered glycosylation of proteins is a cancer-related marker and the monitoring of glycosylation status can significantly increase the informative value of protein biomarkers. Herein is presented an analytical method for the investigation of the glycan composition of colorectal cancer (CRC) protein biomarkers applied to different sample types using a lectin-based microarray platform. The sampl ...
DOI:
10.1039/c7ay00159b

12. Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray

Author:
Andrew C. Petersen; Jeannett M. Clampitt; F. Chris Minion
Source:
Veterinary microbiology 2019 pp. -
ISSN:
0378-1135
Subject:
Escherichia coli; Mycoplasma hyopneumoniae; adhesins; antibodies; antigen-antibody reactions; codons; enzyme-linked immunosorbent assay; mycoplasmosis; polymerase chain reaction; protein microarrays; serology; surface proteins; swine
Abstract:
... Pigs harbor several different species of mycoplasmas, of which Mycoplasma hyopneumoniae presents the most significant economic impact on the swine industry. While ELISAs are the predominant diagnostic assay to measure antibody responses during infection with M. hyopneumoniae, the assay itself is only a rough estimate of the total antibody response. It lends little information on pathogen-wide anti ...
DOI:
10.1016/j.vetmic.2019.02.010

13. Antibody Profiling in COVID-19 Patients with Different Severities by Using Spike Variant Protein Microarrays

Author:
Wen-Yu Su; Pin-Xian Du; Harvey M. Santos; Tzong-Shiann Ho; Batuhan Birol Keskin; Chi Ho Pau; An-Ming Yang; Yi-Yu Chou; Hsi-Chang Shih; Guan-Da Syu
Source:
Analytical chemistry 2022 v.94 no.17 pp. 6529-6539
ISSN:
1520-6882
Subject:
COVID-19 infection; analytical chemistry; blood serum; death; disease progression; dose response; drugs; humoral immunity; protein microarrays; viral nonstructural proteins
Abstract:
... The disease progression of COVID-19 varies from mild to severe, even death. However, the link between COVID-19 severities and humoral immune specificities is not clear. Here, we developed a multiplexed spike variant protein microarray (SVPM) and utilized it for quantifying neutralizing activity, drug screening, and profiling humoral immunity. First, we demonstrated the competition between antispik ...
DOI:
10.1021/acs.analchem.1c05567

14. Arabidopsis Protein Microarrays for the High-Throughput Identification of Protein-Protein Interactions

Author:
Sorina C. Popescu; Michael Snyder; Savithramma P. Dinesh-Kumar
Source:
Plant signaling & behavior 2007 v.2 no.5 pp. 416-420
ISSN:
1559-2324
Subject:
Arabidopsis; calmodulin; humans; plant proteins; protein microarrays; protein-protein interactions; yeasts
Abstract:
... Protein microarray technology has emerged as a powerful new approach for the study of thousands of proteins simultaneously. Protein microarrays have been used for a wide variety of applications for the human and yeast systems. In a recent study, we demonstrated that Arabidopsis functional protein microarrays can be generated and employed to characterize the function of plant proteins. The arrayed ...
DOI:
10.4161/psb.2.5.4416
PubMed:
19704619
PubMed Central:
PMC2634232

15. Aryl diazonium salts: a new class of coupling agents for bonding polymers, biomacromolecules and nanoparticles to surfaces

Author:
Samia Mahouche-Chergui; Sarra Gam-Derouich; Claire Mangeney; Mohamed M. Chehimi
Source:
Chemical Society reviews 2011 v.40 no.7 pp. 4143-4166
ISSN:
1460-4744
Subject:
adhesion; aromatic compounds; biosensors; diazonium compounds; industrial applications; nanoparticles; patents; polymerization; polymers; protein microarrays; salts
Abstract:
... This critical review summarizes existing knowledge on the use of diazonium salts as a new generation of surface modifiers and coupling agents for binding synthetic polymers, biomacromolecules, and nanoparticles to surfaces. Polymer grafts can be directly grown at surfaces through the so-called grafting from approaches based on several polymerization methods but can also be pre-formed in solution a ...
DOI:
10.1039/c0cs00179a

16. Aspartate Residues Far from the Active Site Drive O-GlcNAc Transferase Substrate Selection

Author:
Cassandra M. Joiner; Zebulon G. Levine; Chanat Aonbangkhen; Christina M. Woo; Suzanne Walker
Source:
Journal of the American Chemical Society 2019 v.141 no.33 pp. 12974-12978
ISSN:
1520-5126
Subject:
Animalia; active sites; aspartic acid; enzyme substrates; glycosylation; models; neoplasm progression; neoplasms; protein microarrays; proteins; transferases
Abstract:
... O-GlcNAc is an abundant post-translational modification found on nuclear and cytoplasmic proteins in all metazoans. This modification regulates a wide variety of cellular processes, and elevated O-GlcNAc levels have been implicated in cancer progression. A single essential enzyme, O-GlcNAc transferase (OGT), is responsible for all nucleocytoplasmic O-GlcNAcylation. Understanding how this enzyme ch ...
DOI:
10.1021/jacs.9b06061
PubMed:
31373491
PubMed Central:
PMC6849375

17. Assessing Anticancer Potential of Blueberry Flavonoids, Quercetin, Kaempferol, and Gentisic Acid, Through Oxidative Stress and Apoptosis Parameters on HCT-116 Cells

Author:
Ebru Demirel Sezer; Latife Merve Oktay; Elif Karadadaş; Hikmet Memmedov; Nur Selvi Gunel; Eser Sözmen
Source:
Journal of medicinal food 2019 v.22 no.11 pp. 1118-1126
ISSN:
1557-7600
Subject:
2,5-dihydroxybenzoic acid; Vaccinium myrtillus; antineoplastic activity; antineoplastic agents; antioxidant activity; antioxidants; apoptosis; blueberries; cytotoxicity; diet; flow cytometry; fruit extracts; kaempferol; liquid chromatography; neoplasms; oxidants; oxidative stress; polyphenols; protein composition; protein microarrays; proteins; quercetin; tandem mass spectrometry; therapeutics
Abstract:
... In recent years, natural products gained popularity with their anti-inflammatory and antioxidant effects mediated by chemical compounds within their composition. Study results offering them as palliative therapy options in cancer or as anticancer agents with high levels of cytotoxicity brought a new approach to combine cancer treatment protocols with these products. From a different perspective, e ...
DOI:
10.1089/jmf.2019.0098

18. Autoantibody Signature for the Serologic Detection of Ovarian Cancer

Author:
Anderson Karen S.; Cramer Daniel W.; Sibani Sahar; Wallstrom Garrick; Wong Jessica; Park Jin; Qiu Ji; Vitonis Allison; LaBaer Joshua
Source:
Journal of Proteome Research 2015 v.14 no.1 pp. 578-586
ISSN:
1535-3907
Subject:
autoantibodies; autoantigens; biomarkers; immunoglobulin G; microarray technology; ovarian neoplasms; patients; proteome
Abstract:
... Sera from patients with ovarian cancer contain autoantibodies (AAb) to tumor-derived proteins that are potential biomarkers for early detection. To detect AAb, we probed high-density programmable protein microarrays (NAPPA) expressing 5177 candidate tumor antigens with sera from patients with serous ovarian cancer (n = 34 cases/30 controls) and measured bound IgG. Of these, 741 antigens were selec ...
DOI:
10.1021/pr500908n
PubMed:
25365139
PubMed Central:
PMC4334299

19. Autoreactivity of Broadly Neutralizing Influenza Human Antibodies to Human Tissues and Human Proteins

Author:
Surender Khurana; Megan Hahn; Laura Klenow; Hana Golding
Source:
Viruses 2020 v.12 no.10 pp. -
ISSN:
1999-4915
Subject:
Human immunodeficiency virus 1; Orthomyxoviridae; clinical trials; evolution; hemagglutinins; humans; influenza; influenza vaccines; messenger RNA; monoclonal antibodies; neutralization; pituitary gland; protein microarrays; therapeutics
Abstract:
... Broadly neutralizing monoclonal antibodies (bNAbs) against conserved domains in the influenza hemagglutinin are in clinical trials. Several next generation influenza vaccines designed to elicit such bNAbs are also in clinical development. One of the common features of the isolated bNAbs is the use of restricted IgVH repertoire. More than 80% of stem-targeting bNAbs express IgVH1-69, which may indi ...
DOI:
10.3390/v12101140

20. Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins

Author:
Janine Schulte-Zweckel; Tabea Schneidewind; Jose Luis Abad; Andreas Brockmeyer; Petra Janning; Gemma Triola
Source:
Chemical communications 2018 v.54 no.97 pp. 13742-13745
ISSN:
1364-548X
Subject:
apoptosis; autophagy; ceramides; chemical reactions; inflammation; mass spectrometry; protein microarrays; proteins; proteomics
Abstract:
... Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profilin ...
DOI:
10.1039/c8cc05691a

21. Bearing the brunt: Mongolian khulan (Equus hemionus hemionus) are exposed to multiple influenza A strains

Author:
Eirini S. Soilemetzidou; Erwin de Bruin; Kathrin Eschke; Walid Azab; Nikolaus Osterrieder; Gábor Á. Czirják; Bayarbaatar Buuveibaatar; Petra Kaczensky; Marion Koopmans; Chris Walzer; Alex D. Greenwood
Source:
Veterinary microbiology 2020 v.242 pp. 108605
ISSN:
0378-1135
Subject:
Anseriformes; Equus hemionus hemionus; Equus przewalskii; Influenza A virus; antibodies; asses; birds; blood serum; ecosystems; epizootic diseases; equine influenza; hemagglutinins; horses; hosts; mules; protein microarrays; sympatry; Mongolia
Abstract:
... The majority of influenza A virus strains are hosted in nature by avian species in the orders of Anseriformes and Charadriformes. A minority of strains have been able to cross species boundaries and establish themselves in novel non-avian hosts. Influenza viruses of horses, donkeys, and mules represent such successful events of avian to mammal influenza virus adaptation. Mongolia has over 3 millio ...
DOI:
10.1016/j.vetmic.2020.108605

22. Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19)

Author:
Elvira Gonzalez de Mejia; Candice Mazewski; Diego Luna-Vital; Mark Berhow
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
acclimation; animal models; anthocyanins; anti-inflammatory activity; azoxymethane; body weight; carcinogenesis; colon; colorectal neoplasms; delphinidin; dextran; disease control; enzyme-linked immunosorbent assay; freeze drying; gallic acid; gene expression; histology; immune response; inflammation; lentils; mice; neoplasm cells; placebos; polyphenols; protein microarrays; sodium sulfate; spleen
Abstract:
... The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model. BL was obtained from an overnight soak of organic beluga black lentils (1:2 w/v) in water, filtered, frozen, and freeze- ...
DOI:
10.1093/cdn/nzz030.OR04-06-19

23. Cellular heterogeneity and live cell arrays

Author:
Maureen A. Walling; Jason R. E. Shepard
Source:
Chemical Society reviews 2011 v.40 no.7 pp. 4049-4076
ISSN:
1460-4744
Subject:
DNA; clones; monitoring; precision medicine; protein microarrays; systems biology
Abstract:
... In the past decade, the tendency to move from a global, one-size-fits-all treatment philosophy to personalized medicine is based, in part, on the nuanced differences and sub-classifications of disease states. Our knowledge of these varied states stems from not only the ability to diagnose, classify, and perform experiments on cell populations as a whole, but also from new technologies that allow i ...
DOI:
10.1039/c0cs00212g

24. Comparison of the backbone dynamics of a natural and a consensus designed 3-TPR domain

Author:
Jarymowycz, Virginia A.; Cortajarena, Aitziber L.; Regan, Lynne; Stone, Martin J.
Source:
Journal of biomolecular NMR 2008 v.41 no.3 pp. 169-178
ISSN:
0925-2738
Subject:
acids; design; helix-turn-helix motifs; journals; protein microarrays; proteins; tandem repeat sequences; tetratricopeptide repeats
Abstract:
... The tetratricopeptide repeat (TPR) is a 34-amino acid helix-turn-helix motif that occurs in tandem arrays in numerous proteins. Here we compare the backbone dynamics of a natural 3-repeat TPR domain, from the protein UBP, with the behavior of a designed protein CTPR3, which consists of three identical consensus TPR units. Although the three tandem TPR repeats in both CTPR3 and UBP behave as a sing ...
DOI:
10.1007/s10858-008-9250-6

25. Comprehensive Analyses of the Immunoglobulin Proteome for the Classification of Glomerular Diseases

Author:
Jianhua Liu; Yang Li; Jiayu Dai; Baoxu Lin; Chunying Xiao; Xinpeng Zhang; Lin Luo; Tingting Wang; Xiaoying Li; Yao Yu; Shixiao Chen; Lina Wu; Yong Liu; Xiaobo Yu; Xiaosong Qin
Source:
Journal of proteome research 2020 v.19 no.4 pp. 1502-1512
ISSN:
1535-3907
Subject:
biopsy; blood serum; human diseases; immunoglobulins; kidney diseases; mathematical models; microarray technology; normal distribution; patients; proteome; volunteers
Abstract:
... Glomerular diseases, which are currently diagnosed using an invasive renal biopsy, encompass numerous disease subtypes that often display similar clinical manifestations even though they have different therapeutic regimes. Therefore, a noninvasive assay is needed to classify and guide the treatment of glomerular diseases. Here, we develop and apply a high-throughput and quantitative microarray pla ...
DOI:
10.1021/acs.jproteome.9b00748

26. Controlled protein assembly on a switchable surface

Author:
Ying Liu; Li Mu; Baohong Liu; Song Zhang; Pengyuan Yang; Jilie Kong
Source:
Chemical communications 2004 v. no.10 pp. 1194-1195
ISSN:
1364-548X
Subject:
biocomposites; biosensors; chemical compounds; chemical reactions; protein microarrays
Abstract:
... The strategy presented in this work supplies a general method of controlling protein assembly on a switchable low-density SAM, which may open a new way to design functional biocomposite films for biosensors or protein chips. ...
DOI:
10.1039/b400776j

27. Covalently Attaching Hollow Silica Nanoparticles on a COC Surface for the Fabrication of a Three-Dimensional Protein Microarray

Author:
Jian Yan; Changwen Zhao; Yuhong Ma; Wantai Yang
Source:
Biomacromolecules 2022 v.23 no.6 pp. 2614-2623
ISSN:
1526-4602
Subject:
chemical bonding; composite polymers; condensation reactions; detection limit; glass; hydrolysis; immunoassays; immunoglobulin G; microstructure; olefin; protein microarrays; roughness; silane; silica; surface area; transmittance
Abstract:
... Compared to traditional two-dimensional (2D) biochips, three-dimensional (3D) biochips exhibit the advantages of higher probe density and detection sensitivity due to their designable surface microstructure as well as enlarged surface area. In the study, we proposed an approach to prepare a 3D protein chip by deposition of a monolayer of functionalized hollow silica nanoparticles (HSNs) on an acti ...
DOI:
10.1021/acs.biomac.2c00354

28. Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips

Author:
Manfè Valentina; Fleckner Jan; Nørby Lisby Peder; Zhang Bo; Dai Hongjie; Keller Pernille
Source:
BMC biotechnology 2015 v.15 no.1 pp. 73
ISSN:
1472-6750
Subject:
antibodies; blood serum; cytokines; detection limit; enzyme-linked immunosorbent assay; fluorescence; gold; immunoglobulin G; osteoarthritis; patient care; patients; protein content; protein microarrays; rheumatoid arthritis; synovial fluid
Abstract:
... BACKGROUND: Fluorescence-enhancing microarray on plasmonic gold film is an attractive alternative to traditional enzyme-linked immunosorbent assay (ELISA) for cytokine detection because of the increased sensitivity. The assay chemistry is similar to an ELISA sandwich assay, but owing to the gold substrate, cytokine measurements are 10 to 100 times more sensitive and can be multiplexed. Plasmonic p ...
DOI:
10.1186/s12896-015-0186-0
PubMed:
26268325
PubMed Central:
PMC4535377

29. DNA-Corralled Nanodiscs for the Structural and Functional Characterization of Membrane Proteins and Viral Entry

Author:
Zhao Zhao; Meng Zhang; James M. Hogle; William M. Shih; Gerhard Wagner; Mahmoud L. Nasr
Source:
Journal of the American Chemical Society 2018 v.140 no.34 pp. 10639-10643
ISSN:
1520-5126
Subject:
DNA; Enterovirus C; lipids; manufacturing; membrane proteins; models; protein microarrays
Abstract:
... Here we present a modular method for manufacturing large-sized nanodiscs using DNA-origami barrels as scaffolding corrals. Large-sized nanodiscs can be produced by first decorating the inside of DNA barrels with small lipid-bilayer nanodiscs, which open up when adding extra lipid to form large nanodiscs of diameters ∼45 or ∼70 nm as prescribed by the enclosing barrel dimension. Densely packed memb ...
DOI:
10.1021/jacs.8b04638
PubMed:
30094995
PubMed Central:
PMC6206850

30. DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips

Author:
Mei Hu; Yao He; Shiping Song; Juan Yan; Hao-Ting Lu; Li-Xing Weng; Lian-Hui Wang; Chunhai Fan
Source:
Chemical communications 2010 v.46 no.33 pp. 6126-6128
ISSN:
1364-548X
Subject:
biomarkers; chemical bonding; chemical communication; chemical compounds; chemical reactions; fluorescence; fluorescent dyes; neoplasms; protein microarrays; quantum dots; streptavidin
Abstract:
... A DNA-bridged strategy is used to facilely conjugate streptavidin (STV) to luminescent semiconductor quantum dots (QDs), which leads to convenient and stable QD–DNA–biotin–STV conjugates that serve as fluorescent nanoprobes for ultrasensitive detection of cancer biomarkers with a microfluidic protein chip. ...
DOI:
10.1039/c0cc01608j

31. Detection, quantification, and profiling of PSA: current microarray technologies and future directions

Author:
Mukesh Digambar Sonawane; Satish Balasaheb Nimse; Keum-Soo Song; Taisun Kim
Source:
RSC advances 2016 v.6 no.9 pp. 7599-7609
ISSN:
2046-2069
Subject:
DNA microarrays; biomarkers; blood; death; men; monitoring; mortality; prostate gland; prostatic neoplasms; protein microarrays; therapeutics
Abstract:
... Prostate cancer (PCa) is a cancer of the prostate gland. The death rate of 13% among the men diagnosed with PCa makes it the second leading cause of cancer death. It has been reported that the monitoring of the progression of PCa and response to therapy is done by measuring the level of blood PSA. Even though PSA has been used for the diagnosis of PCa, the current scenario dictates the necessity o ...
DOI:
10.1039/c5ra20313a

32. Development and Application of Human Coronavirus Protein Microarray for Specificity Analysis

Author:
Pin-Xian Du; Yi-Yu Chou; Harvey M. Santos; Batuhan Birol Keskin; Miao-Hsi Hsieh; Tzong-Shiann Ho; Jiu-Yao Wang; Yi-Ling Lin; Guan-Da Syu
Source:
Analytical chemistry 2021 v.93 no.21 pp. 7690-7698
ISSN:
1520-6882
Subject:
COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; analytical chemistry; biological development; biomarkers; blood serum; cross reaction; drugs; humans; influenza; nucleocapsid proteins; protein microarrays; surfactant proteins
Abstract:
... Coronavirus is an enveloped RNA virus that causes mild to severe respiratory diseases in humans, including HKU1-CoV, 229E-CoV, NL63-CoV, OC43-CoV, SARS-CoV, MERS-CoV, and SARS-CoV-2. Due to the outbreak of SARS-CoV-2, it is important to identify the patients and investigate their immune responses. Protein microarray is one of the best platforms to profile the antibodies in the blood because of its ...
DOI:
10.1021/acs.analchem.1c00614

33. Development and application of a novel triplex protein microarray method for rapid detection of antibodies against avian influenza virus, Newcastle disease virus, and avian infectious bronchitis virus

Author:
Yuan Li; Jianhua Hu; Jing Lei; Wentao Fan; Zhenwei Bi; Suquan Song; Liping Yan
Source:
Archives of virology 2021 v.166 no.4 pp. 1113-1124
ISSN:
0304-8608
Subject:
Avian leukosis virus; Avian orthoavulavirus 1; Chicken anemia virus; Infectious bronchitis virus; Infectious bursal disease virus; Influenza A virus; antibodies; blood serum; chickens; cross reaction; enzyme-linked immunosorbent assay; hemagglutination; phosphoproteins; poultry industry; protein microarrays; rapid methods; vaccination; viral nonstructural proteins; virology
Abstract:
... Avian influenza virus (AIV), Newcastle disease virus (NDV), and avian infectious bronchitis virus (IBV) inflict immense damage on the global poultry industry annually. Serological diagnostic methods are fundamental for the effective control and prevention of outbreaks caused by these viruses. In this study, a novel triplex protein microarray assay was developed and validated for the rapid and simu ...
DOI:
10.1007/s00705-021-04962-x

34. Development and validation of different indirect ELISAs for MERS-CoV serological testing

Author:
Anwar M. Hashem; Sawsan S. Al-amri; Tagreed L. Al-subhi; Loai A. Siddiq; Ahmed M. Hassan; Maha M. Alawi; Rowa Y. Alhabbab; Salwa I. Hindawi; Osama B. Mohammed; Nabil S. Amor; Abdulaziz N. Alagaili; Ahmed A. Mirza; Esam I. Azhar
Source:
Journal of immunological methods 2019 v.466 pp. 41-46
ISSN:
0022-1759
Subject:
Middle East respiratory syndrome coronavirus; amino acids; blood serum; enzyme-linked immunosorbent assay; equipment; fluorescent antibody technique; human resources; humans; monitoring; mortality; neutralization; neutralization tests; nucleocapsid proteins; protein microarrays; seroprevalence; Saudi Arabia
Abstract:
... Since 2012, MERS-CoV has caused up to 2220 cases and 790 deaths in 27 countries with Saudi Arabia being the most affected country with ~83.1% of the cases and ~38.8% local death rate. Current serological assays such as microneutralization (MN), plaque reduction neutralization, immunofluorescence, protein microarray or pseudoparticle neutralization assays rely on handling of live MERS-CoV in high c ...
DOI:
10.1016/j.jim.2019.01.005
PubMed:
30659836
PubMed Central:
PMC7094657

35. Development of SARS-CoV-2 variant protein microarray for profiling humoral immunity in vaccinated subjects

Author:
Tzong-Shiann Ho; Pin-Xian Du; Wen-Yu Su; Harvey M. Santos; Ya-Lan Lin; Yi-Yu Chou; Batuhan Birol Keskin; Chi Ho Pau; Guan-Da Syu
Source:
Biosensors & bioelectronics 2022 v.204 pp. 114067
ISSN:
0956-5663
Subject:
Severe acute respiratory syndrome coronavirus 2; antibodies; biosensors; drug development; humoral immunity; neutralization; neutralization tests; people; protein microarrays; vaccination; vaccines
Abstract:
... SARS-CoV-2 is quickly evolving from wild-type to many variants and spreading around the globe. Since many people have been vaccinated with various types of vaccines, it is crucial to develop a high throughput platform for measuring the antibody responses and surrogate neutralizing activities against multiple SARS-CoV-2 variants. To meet this need, the present study developed a SARS-CoV-2 variant ( ...
DOI:
10.1016/j.bios.2022.114067

36. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

Author:
Samuel White; Meriel Moore‐Colyer; Eliane Marti; Laurent Coüetil; Duncan Hannant; Eric A. Richard; Marcos Alcocer
Source:
Journal of veterinary internal medicine 2019 v.33 no.5 pp. 2327-2335
ISSN:
0891-6640
Subject:
allergens; arthropods; asthma; bacteria; filters; fungi; horses; immunoglobulin E; mice; pollen; protein microarrays
Abstract:
... BACKGROUND: Severe asthma in horses, known as severe equine asthma (SEA), is a prevalent, performance‐limiting disease associated with increased allergen‐specific immunoglobulin E (IgE) against a range of environmental aeroallergens. OBJECTIVE: To develop a protein microarray platform to profile IgE against a range of proven and novel environmental proteins in SEA‐affected horses. ANIMALS: Six SEA ...
DOI:
10.1111/jvim.15564
PubMed:
31429513
PubMed Central:
PMC6766494

37. Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays

Author:
Popescu, Sorina C.; Popescu, George V.; Bachan, Shawn; Zhang, Zimei; Seay, Montrell; Gerstein, Mark; Snyder, Michael; Dinesh-Kumar, S.P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.11 pp. 4730-4735
ISSN:
0027-8424
Subject:
Arabidopsis thaliana; protein binding; proteome; open reading frames; protein-protein interactions; calmodulin; plant proteins
Abstract:
... Calmodulins (CaMs) are the most ubiquitous calcium sensors in eukaryotes. A number of CaM-binding proteins have been identified through classical methods, and many proteins have been predicted to bind CaMs based on their structural homology with known targets. However, multicellular organisms typically contain many CaM-like (CML) proteins, and a global identification of their targets and specifici ...
DOI:
10.1073/pnas.0611615104
PubMed:
17360592
PubMed Central:
PMC1838668

38. Effect of nutritional supplement on bone marrow-derived mesenchymal stem cells from aplastic anaemia

Author:
Shihua Luo; Yinghai Chen; Lifen Zhao; Xia Qi; Xiaoyan Miao; Huimin Zhou; Li Jia
Source:
British journal of nutrition 2018 v.119 no.7 pp. 748-758
ISSN:
1475-2662
Subject:
X-radiation; adipocytes; amino acids; anemia; animal models; bioactive properties; blood composition; blood serum; bone density; bone formation; bone marrow; chloramphenicol; cyclophosphamide; dietary supplements; dose response; gene expression regulation; hematopoiesis; hematopoietic stem cells; intragastric administration; mesenchymal stromal cells; nucleotides; nutritional support; protein microarrays; protein synthesis; rats; vitamins
Abstract:
... Aplastic anaemia (AA) is characterised by pancytopenia resulting from a marked reduction in haemopoietic stem cells (HSC). The regulation of haemopoiesis depends on the interaction between HSC and various cells of the bone marrow (BM) microenvironment, including BM-derived mesenchymal stromal cells (BMSC). The purpose of this study was to analyse the biological effect of nutritional supplement (NS ...
DOI:
10.1017/S0007114518000399

39. Establishment and validation of a drug-target microarray for SARS-CoV-2

Author:
Peng Chen; Zehua Zeng; Hongwu Du
Source:
Biochemical and biophysical research communications 2020 v.530 no.1 pp. 4-9
ISSN:
0006-291X
Subject:
COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; humans; pharmacology; politics; protein microarrays; research; therapeutics; viruses; China
Abstract:
... COVID-19 has become one of the worst epidemic in the world, currently already more than four million people have been infected, which probably co-exist with human beings, and has a significant impact on the global economy and political order. In the process of fighting against the epidemic in China, the clinical value of a variety of herbal medicines has been recognized and written into the clinic ...
DOI:
10.1016/j.bbrc.2020.05.217

40. Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax

Author:
Seong-Kyun Lee; Jin-Hee Han; Ji-Hoon Park; Kwon-Soo Ha; Won Sun Park; Seok-Ho Hong; Sunghun Na; Yang Cheng; Eun-Taek Han
Source:
Parasites & vectors 2019 v.12 no.1 pp. 594
ISSN:
1756-3305
Subject:
Plasmodium vivax; amino acids; bioinformatics; blood serum; cell free system; cell-free protein synthesis; computer software; erythrocytes; genes; germ cells; immunization; immunoglobulin G; malaria; membrane proteins; parasites; pathogens; patients; protein microarrays; protein structure; vaccine development; wheat germ
Abstract:
... BACKGROUND: Malaria parasites form intracellular membranes that separate the parasite from the internal space of erythrocytes, and membrane proteins from the parasites are exported to the host via the membrane. In our previous study, Plasmodium vivax early transcribed membrane protein (PvETRAMP) 11.2, an intracellular membrane protein that is highly expressed in blood-stage parasites, was characte ...
DOI:
10.1186/s13071-019-3846-4
PubMed:
31856917
PubMed Central:
PMC6921578

41. FTIR Imaging of Protein Microarrays for High Throughput Secondary Structure Determination

Author:
Joëlle De Meutter; Erik Goormaghtigh
Source:
Analytical chemistry 2021 v.93 no.8 pp. 3733-3741
ISSN:
1520-6882
Subject:
Fourier transform infrared spectroscopy; analytical chemistry; least squares; prediction; protein microarrays; protein secondary structure; support vector machines
Abstract:
... The paper introduces a new method designed for high-throughput protein structure determination. It is based on spotting proteins as microarrays at a density of ca. 2000–4000 samples per cm² and recording Fourier transform infrared (FTIR) spectra by FTIR imaging. It also introduces a new protein library, called cSP92, which contains 92 well-characterized proteins. It has been designed to cover as w ...
DOI:
10.1021/acs.analchem.0c03677

42. Facile Functionalization Strategy for Ultrasensitive Organic Protein Biochips in Multi-Biomarker Determination

Author:
Chenfang Sun; Manikkedath V. Vinayak; Shanshan Cheng; Wenping Hu
Source:
Analytical chemistry 2021 v.93 no.32 pp. 11305-11311
ISSN:
1520-6882
Subject:
analytical chemistry; antigens; biocompatibility; biomarkers; condensation reactions; cross-linking reagents; gelation; glutaraldehyde; liver neoplasms; polymers; protein microarrays
Abstract:
... In recent years, organic field-effect transistors (OFETs) have shown great potential for advanced protein biochips due to their inherent biocompatibility and high-throughput detectability. However, the development of OFET-based protein biochips is still at an early stage. On the one hand, single-biomarker determination is not sufficient for the diagnosis of cancer; thus, simultaneous monitoring of ...
DOI:
10.1021/acs.analchem.1c02601

43. Fluorescence Interference Contrast-enabled structures improve the microarrays performance

Author:
S. Dobroiu; F.C.M.J.M. van Delft; J. Aveyard-Hanson; Prasad Shetty; D.V. Nicolau
Source:
Biosensors & bioelectronics 2019 v.123 pp. 251-259
ISSN:
0956-5663
Subject:
biosensors; diagnostic techniques; fluorescence; fluorescent dyes; kinesin; molecular biology; platinum; protein microarrays; screening; silica; silicon
Abstract:
... Continuous improvements of the fluorescence-based sensitivity and specificity, required for high throughput screening, diagnostics, and molecular biology studies, are usually addressed by better readout systems, or better reporting elements. However, while Fluorescence Interference Contrast (FLIC), which modulates the fluorescence by materials-based parameters, has been used for decades to measure ...
DOI:
10.1016/j.bios.2018.09.009

44. Fluorescence-based kinetic analysis of miniaturized protein microarrays

Author:
Heman Kumar Ramiya Ramesh Babu; Levi A. Gheber
Source:
Biosensors & bioelectronics 2018 v.122 pp. 290-299
ISSN:
0956-5663
Subject:
biosensors; fluorescence; kinetics; microfluidic technology; monitoring; protein microarrays
Abstract:
... Ideal monitoring devices should enjoy a combination of characteristics, e.g. high sensitivity, multiplexing, portability, short time-to-result (TTR). Typically, no device meets all of these demands since some of them are contradictory, to some extent. Herein, we present a miniaturized platform based on fluorescent detection, which is sensitive, readily allows multiplexing, and allows real-time mon ...
DOI:
10.1016/j.bios.2018.09.051

45. Gain-Scanning for Protein Microarray Assays

Author:
Feng Feng; Sila Toksoz Ataca; Mingxuan Ran; Yumei Wang; Michael Breen; Thomas B. Kepler
Source:
Journal of proteome research 2020 v.19 no.7 pp. 2664-2675
ISSN:
1535-3907
Subject:
antibody specificity; biomarkers; blood serum; data collection; humans; immune response; information processing; microbial detection; models; monoclonal antibodies; protein binding; protein microarrays; proteins; proteome
Abstract:
... Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we describe and demonstrate a new data processing procedure, gain-scan, in which data were ...
DOI:
10.1021/acs.jproteome.9b00892

46. Generation of Arabidopsis protein chips for antibody and serum screening

Author:
Kersten, B.; Feilner, T.; Kramer, A.; Wehrmeyer, S.; Possling, A.; Witt, I.; Zanor, M.I.; Stracke, R.; Lueking, A.; Kreutzberger, J.
Source:
Plant molecular biology 2003 v.52 no.5 pp. 999-1010
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; plant proteins; proteomics; protein composition; analytical methods; complementary DNA; cDNA libraries; monoclonal antibodies; antiserum; transcription factors; immunochemistry
Abstract:
... Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several applications on protein chips have been described, but to our knowledge no studi ...
PubMed:
14558660

47. Genetic conservation of Cytauxzoon felis antigens and mRNA expression in the schizont life-stage

Author:
Daven B. Khana; David S. Peterson; James B. Stanton; Megan E. Schreeg; Adam J. Birkenheuer; Jaime L. Tarigo
Source:
Veterinary parasitology 2018 v.263 pp. 49-53
ISSN:
0304-4017
Subject:
Cytauxzoon felis; Protozoa; Theileria; animal tissues; antigens; cat diseases; cats; gene expression; genome; germplasm conservation; immune response; in situ hybridization; messenger RNA; morbidity; mortality; parasites; protein microarrays; sequence analysis; vaccine development; vaccines
Abstract:
... Cytauxzoonosis is a highly fatal disease of domestic cats caused by the apicomplexan protozoan Cytauxzoon felis, which is most closely related to Theileria spp. The growing prevalence, high morbidity and mortality, and treatment cost of cytauxzoonosis emphasize the need for vaccine development. Traditional approaches for vaccine development, however, have been hindered by the inability to culture ...
DOI:
10.1016/j.vetpar.2018.10.007

48. Genetic diversity and immunogenicity of the merozoite surface protein 1 C-terminal 19-kDa fragment of Plasmodium ovale imported from Africa into China

Author:
Qinwen Xu; Sihong Liu; Kokouvi Kassegne; Bo Yang; Jiachen Lu; Yifan Sun; Wenli Zhong; Miaosa Zhang; Yaobao Liu; Guoding Zhu; Jun Cao; Yang Cheng
Source:
Parasites & vectors 2021 v.14 no.1 pp. 583
ISSN:
1756-3305
Subject:
DNA; Escherichia coli; Plasmodium falciparum; Plasmodium ovale; T-lymphocytes; Western blotting; antibodies; blood serum; cross reaction; enzyme-linked immunosorbent assay; erythrocytes; genes; genetic variation; immunogenicity; lymphocyte proliferation; malaria; merozoites; polymerase chain reaction; protein microarrays; splenocytes; surface proteins; vaccine development; vaccines; Africa; China
Abstract:
... BACKGROUND: Merozoite surface protein 1 (MSP1) plays an essential role in erythrocyte invasion by malaria parasites. The C-terminal 19-kDa region of MSP1 has long been considered one of the major candidate antigens for a malaria blood-stage vaccine against Plasmodium falciparum. However, there is limited information on the C-terminal 19-kDa region of Plasmodium ovale MSP1 (PoMSP1₁₉). This study ai ...
DOI:
10.1186/s13071-021-05086-6

49. High Density Diffusion-Free Nanowell Arrays

Author:
Takulapalli Bharath R.; Qiu Ji; Magee D. Mitchell; Kahn Peter; Brunner Al; Barker Kristi; Means Steven; Miersch Shane; Bian Xiaofang; Mendoza Alex; Festa Fernanda; Syal Karan; Park Jin G.; LaBaer Joshua; Wiktor Peter
Source:
Journal of Proteome Research 2012 v.11 no.8 pp. 4382-4391
ISSN:
1535-3907
Subject:
microarray technology; nucleic acids; protein synthesis; protein-protein interactions; proteins; proteome; proteomics; silicon
Abstract:
... Proteomics aspires to elucidate the functions of all proteins. Protein microarrays provide an important step by enabling high-throughput studies of displayed proteins. However, many functional assays of proteins include untethered intermediates or products, which could frustrate the use of planar arrays at very high densities because of diffusion to neighboring features. The nucleic acid programma ...
DOI:
10.1021/pr300467q
PubMed:
22742968
PubMed Central:
PMC3412896

50. High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors

Author:
Dylan W. Domaille; Ju Hun Lee; Jennifer N. Cha
Source:
Chemical communications 2013 v.49 no.17 pp. 1759-1761
ISSN:
1364-548X
Subject:
DNA; bacteriophages; biosensors; catalytic activity; chemical communication; chemical reactions; colorimetry; fluorescent proteins; hydrazones; oligonucleotides; protein microarrays
Abstract:
... We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage–DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild co ...
DOI:
10.1039/c3cc38871a