serineC-palmitoyltransferase, etc ; biosynthesis; pyridoxal phosphate; sphingolipids; spectroscopy; enzymology; Show all 6 Subjects
... Serine palmitoyltransferase, which is one of the α-oxamine synthase family enzymes, catalyzes the condensation reaction of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine, the first and rate-determining step of the sphingolipid biosynthesis. As with other α-oxamine synthase family enzymes, the catalytic reaction is composed of multiple elementary steps, and the mechanism to control the ...
serineC-palmitoyltransferase, etc ; biosynthesis; catalytic activity; chemical communication; chemical reactions; moieties; phosphates; pyridoxal; pyridoxal phosphate; pyridoxine; serine; sphingolipids; substrate specificity; Show all 13 Subjects
... The pyridoxal 5′-phosphate (PLP)-dependent enzyme serine palmitoyltransferase (SPT) is required for de novo sphingolipid biosynthesis. A previous study revealed a novel and unexpected interaction between the hydroxyl group of the l-serine substrate and the 5′-phosphate group of PLP. By using pyridoxal (PL), the dephosphorylated analogue of vitamin B₆, we show here that this interaction is importan ...
Wadsworth, John M.; Clarke, David J.; McMahon, Stephen
A.; Lowther, Jonathan P.; Beattie, Ashley E.; Langridge-Smith, Pat R. R.; Broughton, Howard B.; Dunn, Teresa M.; Naismith, James H.; Campopiano, Dominic J.
serineC-palmitoyltransferase, etc ; X-ray diffraction; active sites; aldehydes; biosynthesis; cell membranes; enzyme inhibitors; fungi; lysine; mass spectrometry; pyridoxal phosphate; serine; sphingolipids; Show all 13 Subjects
... Sphingolipids (SLs) are essential components of cellular membranes formed from the condensation of l-serine and a long-chain acyl thioester. This first step is catalyzed by the pyridoxal-5′-phosphate (PLP)-dependent enzyme serine palmitoyltransferase (SPT) which is a promising therapeutic target. The fungal natural product myriocin is a potent inhibitor of SPT and is widely used to block SL biosyn ...