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You searched for: Subject term quantitative polymerase chain reaction Remove constraint Subject term: quantitative polymerase chain reaction
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1. Co-occurrence of genes for antibiotic resistance and arsenic biotransformation in paddy soils

Author:
Huiling Cui; Dong Zhu; Longjun Ding; Yifei Wang; Jianqiang Su; Guilan Duan; Yongguan Zhu
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 701-711
ISSN:
1001-0742
Subject:
Clostridium; antibiotic resistance; biotransformation; food safety; manure amendments; manure spreading; methylation; multiple drug resistance; paddies; paddy soils; pollution; quantitative polymerase chain reaction; risk; China
Abstract:
... Paddy soils are potential hotspots of combined contamination with arsenic (As) and antibiotics, which may induce co-selection of antibiotic resistance genes (ARGs) and As biotransformation genes (ABGs), resulting in dissemination of antimicrobial resistance and modification in As biogeochemical cycling. So far, little information is available for these co-selection processes and specific patterns ...
DOI:
10.1016/j.jes.2022.02.027

2. Exploring the abundance and influencing factors of antimicrobial resistance genes in manure plasmidome from swine farms

Author:
Zunfang Tu; Junrui Shui; Jinxin Liu; Hongmei Tuo; Haoyu Zhang; Cong Lin; Jingyi Feng; Yuxuan Feng; Wen Su; Anyun Zhang
Source:
Journal of environmental sciences (China) 2023 v.124 pp. 462-471
ISSN:
1001-0742
Subject:
antibiotic resistance; farms; florfenicol; herds; pig manure; plasmids; potassium; public health; quantitative polymerase chain reaction; swine; swine production; total phosphorus; China
Abstract:
... Plasmids play a critical role in the dissemination of antimicrobial resistance genes (ARGs), however, a systematical understanding of ARGs originated from plasmids in swine production is currently lacking. Herein, quantitative polymerase chain reaction was applied to determine the prevalence of ten ARGs and the class1 integron gene intI1 of plasmid source in swine manure from 44 farms in Sichuan, ...
DOI:
10.1016/j.jes.2021.11.030

3. Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae

Author:
Chao Shen; Yimei Cai; Jialing Li; Chengyong He; Zhenghong Zuo
Source:
Journal of environmental sciences (China) 2023 v.124 pp. 76-88
ISSN:
1001-0742
Subject:
Danio rerio; anilinopyrimidine fungicides; aryl hydrocarbon receptors; developmental toxicity; fruit growing; fungi; genes; light intensity; mepanipyrim; opsin; phototransduction; quantitative polymerase chain reaction; transcriptomics; wavelengths; China
Abstract:
... Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked ...
DOI:
10.1016/j.jes.2021.11.008

4. NirS-type denitrifying bacteria in aerobic water layers of two drinking water reservoirs: Insights into the abundance, community diversity and co-existence model

Author:
Haihan Zhang; Yinjie Shi; Tinglin Huang; Rongrong Zong; Zhenfang Zhao; Ben Ma; Nan Li; Shangye Yang; Mengqiao Liu
Source:
Journal of environmental sciences (China) 2023 v.124 pp. 215-226
ISSN:
1001-0742
Subject:
Azoarcus; Cupriavidus necator; Dechloromonas; Paracoccus denitrificans; Pseudomonas stutzeri; Staphylococcus; Thauera; bacterial communities; chlorophyll; community structure; denitrification; genes; keystone species; models; nitrogen; pH; quantitative polymerase chain reaction; turbidity; water quality; water reservoirs; water temperature; China
Abstract:
... The nirS-type denitrifying bacterial community is the main drivers of the nitrogen loss process in drinking water reservoir ecosystems. The temporal patterns in nirS gene abundance and nirS-type denitrifying bacterial community harbored in aerobic water layers of drinking water reservoirs have not been studied well. In this study, quantitative polymerase chain reaction (qPCR) and Illumina Miseq se ...
DOI:
10.1016/j.jes.2021.10.013

5. Responses of potential ammonia oxidation and ammonia oxidizers community to arsenic stress in seven types of soil

Author:
Yanan Wang; Xibai Zeng; Yang Zhang; Nan Zhang; Liyang Xu; Cuixia Wu
Source:
Journal of environmental sciences (China) 2023 v.127 pp. 15-29
ISSN:
1001-0742
Subject:
Nitrosospira; ammonia; arsenic; bioavailability; community structure; genes; humans; nitrogen; oxidation; phylogeny; quantitative polymerase chain reaction; restriction fragment length polymorphism; toxicity; China
Abstract:
... Soil arsenic contamination is of great concern because of its toxicity to human, crops, and soil microorganisms. However, the impacts of arsenic on soil ammonia oxidizers communities remain unclear. Seven types of soil spiked with 0 or 100 mg arsenic per kg soil were incubated for 180 days and sampled at days 1, 15, 30, 90 and 180. The changes in the community composition and abundance of ammonia ...
DOI:
10.1016/j.jes.2022.02.038

6. (+)-Cyanidan-3-ol inhibits epidermoid squamous cell carcinoma growth via inhibiting AKT/mTOR signaling through modulating CIP2A-PP2A axis

Author:
Jitender Monga; Sharad Kumar Suthar; Deepak Rohila; Alex Joseph; Chetan Singh Chauhan; Manu Sharma
Source:
Phytomedicine 2022 v.101 pp. 154116
ISSN:
0944-7113
Subject:
agar; apoptosis; cell cycle checkpoints; dephosphorylation; drug resistance; experimental design; flow cytometry; fluorescent antibody technique; histology; humans; models; molecular biology; papilloma; phytotherapy; quantitative polymerase chain reaction; skin (animal); skin neoplasms; squamous cell carcinoma; toxicity; xenotransplantation
Abstract:
... Background: Despite recent advances in the treatment of squamous cell skin cancer (SCSC), the disease persists, and treatment resistance develops. Thus, identifying new targets and developing new therapeutic approaches showing low vulnerability to drug resistance is highly needed. Purpose: This study aimed to reveal a novel targeted phytotherapeutic strategy for SCSC treatment alone or in combinat ...
DOI:
10.1016/j.phymed.2022.154116

7. (±)-Equol interferes with the secretion of gonadotropin-releasing hormone and the expression of neuromodulators in murine neurons

Author:
Jingyuan Xiong; Minghui Wang; Ye Tian; Aru Ling; Shufang Shan; Li Zhao; Guo Cheng
Source:
Toxicological and environmental chemistry 2022 v.104 no.1 pp. 129-140
ISSN:
1029-0486
Subject:
RING finger domains; Western blotting; cell viability; daidzein; environmental science; enzyme-linked immunosorbent assay; estrogens; females; gonadotropin-releasing hormone; intestines; menopause; metabolites; mice; neurotransmitters; quantitative polymerase chain reaction; secretion; toxicology
Abstract:
... S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the ef ...
DOI:
10.1080/02772248.2022.2055031

8. 17β-Trenbolone binds to androgen receptor, decreases number of primordial germ cells, modulates expression of genes related to sexual differentiation, and affects sexual differentiation in zebrafish (Danio rerio)

Author:
Hua Tian; Rui Liu; Suqiu Zhang; Shuhui Wei; Wei Wang; Shaoguo Ru
Source:
Science of the total environment 2022 v.806 pp. 150959
ISSN:
0048-9697
Subject:
Danio rerio; androgen receptors; energy; environment; enzyme-linked immunosorbent assay; fish; fluorescence; genetically modified organisms; hybridization; quantitative polymerase chain reaction; sex ratio; sexual development; testes; vitellogenin
Abstract:
... Exposure to 17β-trenbolone caused a skewed sex ratio in fish. However, the molecular initiating event and key molecular event(s) remain unknown. In this study, zebrafish were exposed to 17β-trenbolone at nominal concentrations of 2 ng/L, 20 ng/L, 200 ng/L, and 2000 ng/L from fertilization to 60 days post fertilization (dpf). First, the sex ratio at 60 dpf was calculated to evaluate adverse outcome ...
DOI:
10.1016/j.scitotenv.2021.150959

9. 2-tert-Butyl-1,4-benzoquinone, a food additive oxidant, reduces virulence factors of Chromobacterium violaceum

Author:
Kai-Zhong Xu; Xiao-Juan Tan; Zeng-Yan Chang; Jun-Jian Li; Ai-Qun Jia
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.163 pp. 113569
ISSN:
0023-6438
Subject:
Caenorhabditis elegans; Chromobacterium violaceum; Salvia hispanica; animal models; biofilm; chitinase; ciprofloxacin; food additives; food industry; hemolysins; hydroquinone; multiple drug resistance; oxidants; quantitative polymerase chain reaction; survival rate; violacein; virulence
Abstract:
... Multi-drug resistant bacteria have been paying increasing attentions. Quorum sensing (QS) and QS-associated biofilms are showing the vital reasons for antibiotics resistant. In this study, 2-tert-butyl-1,4-benzoquinone (TBQ), the oxidant of tert-butyl hydroquinone (TBHQ), can potentially be the QS inhibitor (QSI) of Chromobacterium violaceum ATCC12472. After treated with 50 μg/mL of TBQ, the viola ...
DOI:
10.1016/j.lwt.2022.113569

10. In vitro transmission of Chlamydia using naturally infected koala (Phascolarctos cinereus) semen

Author:
Lyndal Hulse; Kenneth W. Beagley; Rebecca Larkin; Stephen D. Johnston
Source:
Reproduction, fertility, and development 2022 v.34 no.3 pp. 378-384
ISSN:
1031-3613
Subject:
Chlamydia; Chlamydophila pecorum; Phascolarctos cinereus; cell lines; immune response; quantitative polymerase chain reaction; reproduction; semen
Abstract:
... Transmission of Chlamydia pecorum infection has generally been assumed to be via the urogenital route and in an attempt to confirm this we investigated an in vitro method of Chlamydia infection using naturally infected koala semen to inoculate a cell line and attempt to estimate C. pecorum infectious load. A total of 57% of 122 koala semen samples had low C. pecorum copy number or no burden, while ...
DOI:
10.1071/RD21233

11. A 12-bp indel in the 3’UTR of porcine CISH gene associated with Landrace piglet diarrhea score

Author:
Buyue Niu; Zhihua Chen; Diwen Yao; Mingxing Kou; Xiaowen Gao; Yuan Sun; Xiuqin Yang; Xibiao Wang; Shengwei Di; Jiancheng Cai; Dongchun Guo
Source:
Research in veterinary science 2022 v.146 pp. 53-59
ISSN:
0034-5288
Subject:
colon; computer simulation; cytokines; diarrhea; gene expression; genes; genotype; humans; ileum; landraces; luciferase; lungs; mutation; phenotype; piglets; plasmids; quantitative polymerase chain reaction; research; statistical analysis; veterinary medicine
Abstract:
... Variations in Cytokine inducible SH2-containing protein (CISH) gene influence human susceptibility to common infectious diseases, but little is known about CISH in swine. The objectives of this study were to 1) determine porcine CISH (pCISH) mRNA expression level in different tissues of piglets, 2) predict putative functional genetic variations within pCISH, 3) investigate the association between ...
DOI:
10.1016/j.rvsc.2022.03.012

12. A 15-year monitoring of Rickettsiales (Anaplasma phagocytophilum and Rickettsia spp.) in questing ticks in the city of Hanover, Germany

Author:
Antje Glass; Andrea Springer; Christina Strube
Source:
Ticks and tick-borne diseases 2022 v.13 no.5 pp. 101975
ISSN:
1877-959X
Subject:
Anaplasma phagocytophilum; Ixodes ricinus; Rickettsia; adults; climate change; females; pathogens; quantitative polymerase chain reaction; ticks; Germany
Abstract:
... Rickettsiales (Anaplasma phagocytophilum and Rickettsia spp.) are regarded as potentially emerging tick-borne pathogens and may change in abundance in response to global climate change. However, continuous monitoring on their prevalence in questing ticks is only available for the northern German city of Hanover. In the presented follow-up of this long-term study, 2100 questing ticks of the Ixodes ...
DOI:
10.1016/j.ttbdis.2022.101975

13. A Kazal-type serine protease inhibitor mediates innate immunity in wild silkworm Actias selene Hübner

Author:
Na Wang; Fengjiao Xie; Ya Liu; Xiaoli Jiang; Guoqing Wei; Baojian Zhu; Lei Wang; Cen Qian
Source:
Journal of biosciences 2022 v.47 no.2 pp. 34
ISSN:
0973-7138
Subject:
Actias; Baculoviridae; Beauveria bassiana; Bombyx mandarina; Escherichia coli; Micrococcus luteus; enzyme activity; fat body; genes; hemolymph; immunostimulants; innate immunity; insects; monophenol monooxygenase; prophenoloxidase; proteinase inhibitors; quantitative polymerase chain reaction; serine proteinases; silk
Abstract:
... The prophenoloxidase (PPO) activation system is an important innate immune defense mechanism in arthropods. Actias selene is a rare and important wild silk insect that can spin high-quality cocoon silk, but, other than its morphology, its molecular mechanism is rarely reported. Here, we report the purification and characterization of a novel KSPI gene from A. selene (AsKSPI), which can negatively ...
DOI:
10.1007/s12038-022-00272-9

14. A Long-Amplicon Viability-qPCR Test for Quantifying Living Pathogens that Cause Bacterial Spot in Tomato Seed

Author:
Hehe Wang; Rieanna Wagnon; Daniela Moreno; Sujan Timilsina; Jeffery Jones; Gary Vallad; William W. Turechek
Source:
Plant disease 2022 v.106 no.5 pp. 1474-1485
ISSN:
0191-2917
Subject:
DNA; disease-free seeds; inoculum; propidium; quantitative polymerase chain reaction; seed extracts; tomatoes
Abstract:
... Bacterial spot is one of the most serious diseases of tomato. It is caused by four species of Xanthomonas: X. euvesicatoria, X. gardneri, X. perforans, and X. vesicatoria. Contaminated or infected seed can be a major source of inoculum for this disease. The use of certified pathogen-free seed is one of the primary management practices to reduce the inoculum load in commercial production. Current s ...
DOI:
10.1094/PDIS-11-21-2509-RE

15. A Longitudinal Study of the Microbial Basis of Nitrous Oxide Emissions Within a Long-Term Agricultural Experiment

Author:
Gevan D. Behnke; Nakian Kim; Chance W. Riggins; María C. Zabaloy; Sandra L. Rodriguez-Zas; María B. Villamil
Source:
Frontiers in agronomy 2022 v.4 pp. -
ISSN:
2673-3218
Subject:
Glycine max; Secale cereale; Vicia villosa; Zea mays; agronomy; ammonium; chiseling; corn; cover crops; crop rotation; denitrification; longitudinal studies; nitrates; nitrification; nitrogen cycle; nitrogen fixation; nitrous oxide; no-tillage; quantitative polymerase chain reaction; rye; soil pH; soybeans
Abstract:
... Much of the global nitrous oxide emissions are derived from agricultural management driving microbial N transformations. Crop rotation, no-till, and cover cropping are feasible conservation agronomic strategies used to prevent N losses to the environment, though their effect on soil microbial N cycling at the field scale remains relatively unknown. Our goal was to determine the effect of crop rota ...
DOI:
10.3389/fagro.2022.833338

16. A Longitudinal Survey of Antibiotic-Resistant Enterobacterales in the Irish Environment, 2019–2020

Author:
Brigid Hooban; Kelly Fitzhenry; Louise O'Connor; Georgios Miliotis; Aoife Joyce; Alexandra Chueiri; Maeve Louise Farrell; Niall DeLappe; Alma Tuohy; Martin Cormican; Dearbháile Morris
Source:
Science of the total environment 2022 v.828 pp. 154488
ISSN:
0048-9697
Subject:
Escherichia coli; Klebsiella pneumoniae; aminoglycosides; antibiotic resistance; aquatic environment; brackish water; hospitals; longitudinal studies; quantitative polymerase chain reaction; resistance genes; sewage; wastewater; wastewater treatment; Ireland
Abstract:
... The natural environment represents a complex reservoir of antibiotic-resistant bacteria as a consequence of different wastewater discharges including anthropogenic and agricultural. Therefore, the aim of this study was to examine sewage and waters across Ireland for the presence of antibiotic-resistant Enterobacterales. Samples were collected from the West, East and South of Ireland. Two periods o ...
DOI:
10.1016/j.scitotenv.2022.154488

17. A Metabolic Network Mediating the Cycling of Succinate, a Product of ROS Detoxification into α-Ketoglutarate, an Antioxidant

Author:
Félix Legendre; Alex MacLean; Sujeenthar Tharmalingam; Vasu D. Appanna
Source:
Antioxidants 2022 v.11 no.3 pp. -
ISSN:
2076-3921
Subject:
Pseudomonas; antioxidants; bacteria; biochemical pathways; byproducts; electrophoresis; glutamate decarboxylase; metabolites; oxidoreductases; quantitative polymerase chain reaction; soil microorganisms; succinic acid; sulfur
Abstract:
... Sulfur is an essential element for life. However, the soil microbe Pseudomonas (P.) fluorescens can survive in a low sulfur environment. When cultured in a sulfur-deficient medium, the bacterium reprograms its metabolic pathways to produce α-ketoglutarate (KG) and regenerate this keto-acid from succinate, a by-product of ROS detoxification. Succinate semialdehyde dehydrogenase (SSADH) and KG decar ...
DOI:
10.3390/antiox11030560

18. A Molecular and Epidemiological Description of a Severe Porcine Reproductive and Respiratory Syndrome Outbreak in a Commercial Swine Production System in Russia

Author:
Karyn A. Havas; Dennis N. Makau; Sergei Shapovalov; Ekaterina Tolkova; Kim VanderWaal; Tymofii Tkachyk; Gordon D. Spronk; Brad Heron; Scott A. Dee; Andres Perez
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
Aujeszky disease; RNA-directed DNA polymerase; Russia; biosecurity; genetic distance; porcine reproductive and respiratory syndrome; quantitative polymerase chain reaction; swine; swine production; viruses
Abstract:
... Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating disease of swine in many parts of the world. Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 is endemic in Europe, and prevalence of the subtypes differ spatially. In this study, we investigated a severe PRRS outbreak reported in 30 farms located in eastern Russia that belong to a large swine pro ...
DOI:
10.3390/v14020375

19. A New Real-Time PCR Assay for Detecting Fungi in Genus Ceratocystis

Author:
Karthikeyan Dharmaraj; Alice M. Merrall; Julie A. Pattemore; Joanne Mackie; Brett J. R. Alexander; Merje Toome-Heller
Source:
Plant disease 2022 v.106 no.2 pp. 661-668
ISSN:
0191-2917
Subject:
Ceratocystis; DNA; biosecurity; figs; genes; plant germplasm; quantitative polymerase chain reaction
Abstract:
... The genus Ceratocystis contains several significant plant pathogens, causing wilt and canker disease on a wide range of plant species. There are >40 known species of Ceratocystis, some of which are becoming increasingly important in agricultural or natural ecosystems. The diagnostic procedures for most Ceratocystis species rely on time-consuming and labor-intensive culturing approaches. To provide ...
DOI:
10.1094/PDIS-08-21-1639-RE

20. A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells

Author:
Danping Zhang; Xiaoxiao Chen; Dan Zheng
Source:
Applied biochemistry and biotechnology 2022 v.194 no.5 pp. 2061-2076
ISSN:
0273-2289
Subject:
Western blotting; apoptosis; biotechnology; cell viability; cytotoxicity; diabetic nephropathy; fibrosis; flow cytometry; genes; glucose; humans; inflammation; ligands; microRNA; precipitin tests; quantitative polymerase chain reaction
Abstract:
... Long non-coding RNAs (lncRNAs) play crucial roles in the development of diabetic nephropathy (DN). Here, we explored the activity and mechanism of MIR503 host gene (MIR503HG) in high glucose (HG)-evoked cytotoxicity in HK-2 cells. MIR503HG, microRNA (miR)-497-5p, and C–C motif chemokine ligand 19 (CCL19) were quantified by quantitative real-time PCR (qRT-PCR) and western blot. The direct relations ...
DOI:
10.1007/s12010-021-03776-6