Subject: "recombinant DNA" / Subject: 3 selected

1. A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer

Author:: Geest, A.H.M. van der; Hall, T.C.
Source:: Plant molecular biology 1996 v.32 no.4 pp. 579-588
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; Phaseolus vulgaris; phaseolin; reporter genes; beta-glucuronidase; gene expression; endosperm; embryo (plant); transgenic plants; Nicotiana tabacum; promoter regions; histochemistry; Show all 12 Subjects
Abstract:: ... In beans, expression of the 3-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between the phas promoter and the gus-coding region yield expression in both embryo and endos ...
DOI:: 10.1007/BF00020199
PubMed:: 8980510
: http://dx.doi.org/10.1007/BF00020199

2. A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

Author:: Vijn, I.; Christiansen, H.; Lauridsen, P.; Kardailsky, I.; Quandt, H.J.; Broer, I.; Drenth, J.; Jensen, E.O.; Kammen, A. van.; Bisseling, T.
Source:: Plant molecular biology 1995 v.28 no.6 pp. 1103-1110
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; genetic transformation; nodulins; transgenic plants; Vicia hirsuta; root nodules; Rhizobium rhizogenes; gene expression; lipooligosaccharides; histochemistry; reporter genes; Pisum sativum; promoter regions; beta-glucuronidase; Show all 14 Subjects
Abstract:: ... ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in ...
DOI:: 10.1007/BF00032670
PubMed:: 7548827
: http://dx.doi.org/10.1007/BF00032670

3. A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription

Author:: Hobo, T.; Kowyama, Y.; Hattori, T.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.26 pp. 15348-15353
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; transcription factors; protoplasts; messenger RNA; promoter regions; complementary DNA; Oryza sativa; transgenic plants; embryo (plant); transcription (genetics); gene expression; binding sites; reporter genes; abscisic acid; roots; beta-glucuronidase; leaves; Show all 17 Subjects
Abstract:: ... The transcription factor VP1 regulates maturation and dormancy in plant seeds by activating genes responsive to the stress hormone abscisic acid (ABA). Although activation involves ABA-responsive elements (ABREs), VP1 itself does not specifically bind ABREs. Instead, we have identified and cloned a basic region leucine zipper (bZIP) factor, TRAB1, that interacts with both VP1 and ABREs. Transcript ...
DOI:: 10.1073/pnas.96.26.15348
PubMed:: 10611387
PubMed Central:: PMC24822
: http://dx.doi.org/10.1073/pnas.96.26.15348

4. A cis-acting element and a trans-acting factor involved in the wound-induced expression of a horseradish peroxidase gene

Author:: Kawaoka, A.; Kawamoto, T.; Sekine, M.; Yoshida, K.; Takano, M.; Shinmyo, A.
Source:: The plant journal 1994 v.6 no.1 pp. 87-97
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; transcription factors; promoter regions; structural genes; complementary DNA; amino acid sequences; gene expression; transcription (genetics); histochemistry; Nicotiana tabacum; DNA-binding proteins; Armoracia rusticana; abiotic stress; reporter genes; peroxidase; beta-glucuronidase; Show all 16 Subjects
DOI:: 10.1046/j.1365-313X.1994.6010087.x
PubMed:: 7920706
: http://dx.doi.org/10.1046/j.1365-313X.1994.6010087.x

5. A clock- and light-regulated gene that links the circadian oscillator to LHCB gene expression

Author:: Xu, Y.; Johnson, C.H.
Source:: plant cell 2001 v.13 no.6 pp. 1411-1425
ISSN:: 1040-4651
Subject:: recombinant DNA, etc ; messenger RNA; complementary DNA; open reading frames; light harvesting complex; luciferase; amino acid sequences; gene expression; Nicotiana tabacum; photoperiod; reporter genes; circadian rhythm; promoter regions; light; Show all 14 Subjects
Abstract:: ... We have identified and characterized a novel tobacco gene, called ZGT (from the Chinese phrase zhong guang tiaokong, or clock and light controlled), that is regulated by the circadian clock and light. ZGT transcripts have alternate forms that are differentially expressed in different tissues. ZGT is expressed rhythmically in light/dark cycles and in constant light. Constitutive expression of ZGT s ...
PubMed:: 11402169
PubMed Central:: PMC135572

6. Activation of basal transcription by a mutation in SIN4, a yeast global repressor, occurs through a mechanism different from activator-mediated transcriptional enhancement

Author:: Mizuno, T.; Harashima, S.
Source:: MGG 2000 v.263 no.1 pp. 48-59
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; Saccharomyces cerevisiae; gene expression; transcription factors; reporter genes; transcription (genetics); mutation; promoter regions; Show all 8 Subjects
DOI:: 10.1007/PL00008675
PubMed:: 10732673
: http://dx.doi.org/10.1007/PL00008675

7. Activation of the promoter of the Tnt1 retrotransposon in tomato after inoulation with the fungal pathogen Cladosporium fulvum

Author:: Mhiri, C.; Wit, P.J.G.M. de.; Grandbastien, M.A.
Source:: Molecular plant-microbe interactions 1999 v.12 no.7 pp. 592-603
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; Solanum lycopersicum var. lycopersicum; retrotransposons; infection; promoter regions; cell walls; fungal diseases of plants; defense mechanisms; transcription (genetics); gene expression; reporter genes; virulence; beta-glucuronidase; leaves; Passalora fulva; Show all 15 Subjects
Abstract:: ... The copia-like Tnt1 element of tobacco is one of the few active plant retrotransposons and is transcriptionally activated, in tobacco and in heterologous species, by biotic and abiotic stress factors. In order to establish more precisely the link between Tnt1 activation and plant defense responses, the expression of the Tnt1 promoter was studied in a gene-for-gene pathosystem, the interaction betw ...
DOI:: 10.1094/MPMI.1999.12.7.592
: http://dx.doi.org/10.1094/MPMI.1999.12.7.592

8. Activity of constitutive promoters in various species from the Liliaceae

Author:: Wilmink, A.; Ven, B.C.E. van de.; Dons, J.J.M.
Source:: Plant molecular biology 1995 v.28 no.5 pp. 949-955
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; Tulipa gesneriana; Lilium longiflorum; Allium ampeloprasum; Oryza sativa; Nicotiana tabacum; Zea mays; Nicotiana plumbaginifolia; transgenic plants; reporter genes; beta-glucuronidase; gene expression; genetic transformation; introns; promoter regions; transcription (genetics); Show all 16 Subjects
Abstract:: ... In this paper we first review literature on the performance of various promoters in monocotyledonous species. In general, promoters isolated from monocots show a higher activity in monocot species. Moreover, the presence of an intron between the promoter and reporter gene increases transcription levels. We used the same approach to study gene expression in Liliaceae. The activities of the CaMV 35S ...
DOI:: 10.1007/BF00042079
PubMed:: 7640366
: http://dx.doi.org/10.1007/BF00042079

9. Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element

Author:: Klein, U.; Salvador, M.L.; Bogorad, L.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.23 pp. 10819-10823
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Chlamydomonas reinhardtii; structural genes; ribulose-bisphosphate carboxylase; reporter genes; beta-glucuronidase; gene expression; promoter regions; transcription (genetics); Show all 9 Subjects
Abstract:: ... The chloroplast gene rbcL encodes the large subunit of ribulose bisphosphate carboxylase. In Chlamydomonas reinhardtii, this gene is transcribed more actively than any other protein-encoding chloroplast gene studied to date. To delineate the rbcL gene promoter, chimeric reporter genes containing fragments of the 5' region of the rbcL gene fused to the coding sequence of the bacterial uidA gene. en ...
DOI:: 10.1073/pnas.91.23.10819
PubMed:: 7971968
PubMed Central:: PMC45117
: http://dx.doi.org/10.1073/pnas.91.23.10819

10. Activity of the promoter of the Lhca3.St.1 gene, encoding the potato apoprotein 2 of the light-harvesting complex of Photosystem I, in transgenic potato and tobacco plants

Author:: Nap, J.P.; Spanje, M. van.; Dirkse, W.G.; Baarda, G.; Mlynarova, L.; Loonen, A.; Grondhuis, P.; Stiekema, W.J.
Source:: Plant molecular biology 1993 v.23 no.3 pp. 605-612
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; genetic transformation; apoproteins; structural genes; transgenic plants; light harvesting complex; Solanum tuberosum; amino acid sequences; gene expression; histochemistry; Nicotiana tabacum; reporter genes; photosystem I; promoter regions; beta-glucuronidase; Show all 15 Subjects
Abstract:: ... We have isolated cDNA and genomic clones for the potato (Solanum tuberosum) apoprotein 2 of the light harvesting complex of Photosystem I, designated Lhca3.St.1. The protein shows all characteristics of the family of chlorophyll a/b-binding proteins. Potato Lhca3.1 gene expression occurs predominantly in leaves, and is transcriptionally regulated by light. One gene copy is present per haploid geno ...
DOI:: 10.1007/BF00019307
PubMed:: 8219093
: http://dx.doi.org/10.1007/BF00019307

11. A cyanobacterial gene encoding peptidyl-prolyl cis-trans isomerase

Author:: Hassidim, M.; Schwarz, R.; Lieman-Hurwitz, J.; Marco, E.; Ronen-Tarazi, M.; Kaplan, A.
Source:: Plant physiology 1992 v.100 no.4 pp. 1982-1986
ISSN:: 0032-0889
Subject:: recombinant DNA, etc ; kanamycin; structural genes; amino acid sequences; gene expression; phosphotransferases (phosphomutases); transcription (genetics); reporter genes; promoter regions; Synechococcus; drug resistance; Show all 11 Subjects
Abstract:: ... The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not ab ...
DOI:: 10.1104/pp.100.4.1982
PubMed:: 16653227
: http://dx.doi.org/10.1104/pp.100.4.1982

12. Adenine methylation at dam sites increases transient gene expression in plant cells

Author:: Graham, M.W.; Larkin, P.J.
Source:: Transgenic research 1995 v.4 no.5 pp. 324-331
ISSN:: 0962-8819
Subject:: recombinant DNA, etc ; Triticum aestivum; Nicotiana plumbaginifolia; Trifolium subterraneum; DNA methylation; adenine; Escherichia coli; gene transfer; genetic transformation; gene expression; reporter genes; beta-glucuronidase; protoplasts; electroporation; promoter regions; Show all 15 Subjects
DOI:: 10.1007/BF01972529
PubMed:: 8589735
: http://dx.doi.org/10.1007/BF01972529

13. A Drosophila gene promoter is subject to glucose repression in yeast cells

Author:: Hickey, D.A.; Benkel, K.I.; Fong, Y.; Benkel, B.F.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.23 pp. 11109-11112
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Drosophila melanogaster; Saccharomyces cerevisiae; alpha-amylase; luciferase; reporter genes; gene expression; glucose; genetic transformation; promoter regions; Show all 10 Subjects
Abstract:: ... Previous work has shown that the alpha-amylase gene of Drosophila melanogaster is subject to repression by dietary glucose. Moreover, glucose repression of this gene is mediated by promoter elements that lie upstream of the transcriptional start site. In this study, we examined the activity of the glucose-repressible Drosophila promoter in transformed yeast cells. We show that the amylase promoter ...
DOI:: 10.1073/pnas.91.23.11109
PubMed:: 7526389
PubMed Central:: PMC45176
: http://dx.doi.org/10.1073/pnas.91.23.11109

14. A heat-inducible Adh gene as a reporter gene for a negative selection in transgenic Arabidopsis

Author:: Severin, K.; Wagner, A.; Schoffl, F.
Source:: Transgenic research 1995 v.4 no.3 pp. 163-172
ISSN:: 0962-8819
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; transgenic plants; alcohol dehydrogenase; reporter genes; heat shock proteins; Glycine max; gene expression; heat; heat stress; enzyme activity; mutants; allyl alcohol; promoter regions; Show all 14 Subjects
DOI:: 10.1007/BF01968781
: http://dx.doi.org/10.1007/BF01968781

15. A homolog of the mammalian GTPase Rab2 is present in Arabidopsis and is expressed predominantly in pollen grains and seedlings

Author:: Moore, I.; Diefenthal, T.; Zarsky, V.; Schell, J.; Palme, K.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.2 pp. 762-767
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; guanosinetriphosphatase; inflorescences; seedlings; pollen; promoter regions; structural genes; binding proteins; gene expression; introns; Arabidopsis thaliana; messenger RNA; pyrophosphatases; amino acid sequences; exons; histochemistry; transgenic plants; guanosine triphosphate; reporter genes; beta-glucuronidase; Show all 20 Subjects
Abstract:: ... Vesicle traffic between the endoplasmic reticulum and the Golgi apparatus in mammals requires the small GTP-binding protein Rab2, but Saccharomyces cerevisiae appears not to have a Rab2 homolog. Here it is shown that the higher plant, Arabidosis thaliana, contains a gene, At-RAB2, whose predicted product shares 79% identity with human Rab2 protein. Transgenic plants containing fusions between beta ...
DOI:: 10.1073/pnas.94.2.762
PubMed:: 9012859
PubMed Central:: PMC19588
: http://dx.doi.org/10.1073/pnas.94.2.762

16. A hormogonium regulating locus, hrmUA, of the cyanobacterium Nostoc punctiforme strain ATCC 29133 and its response to an extract of a symbiotic plant partner Anthoceros punctatus

Author:: Cohen, M.F.; Meeks, J.C.
Source:: Molecular plant-microbe interactions 1997 v.10 no.2 pp. 280-289
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; insertional mutagenesis; symbionts; luciferase; plant extracts; Bryopsida; promoter regions; Nostoc; gametophytes; phenotype; open reading frames; gene expression; amino acid sequences; loci; symbiosis; plant anatomy; reporter genes; Show all 17 Subjects
Abstract:: ... Transposon-generated mutant strain UCD 328 of Nostoc punctiforme strain ATCC 29133 has a phenotype of an increased sensitivity to a hormogonium-inducing factor exuded by a symbiotic plant partner, Anthoceros punctatus, and an initial increased hormogonium-dependent infection of the plant. Sequence analysis showed that the transposition site in strain UCD 328 lies within a 1,251-bp open reading fra ...
DOI:: 10.1094/MPMI.1997.10.2.280
PubMed:: 9057333
: http://dx.doi.org/10.1094/MPMI.1997.10.2.280

17. A luciferase expression system for Physarum that facilitates analysis of regulatory elements

Author:: Bailey, J.; Benard, M.; Burland, T.G.
Source:: Current genetics 1994 v.26 no.2 pp. 126-131
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Physarum polycephalum; gene transfer; luciferase; reporter genes; gene expression; genetic transformation; promoter regions; Show all 8 Subjects
Abstract:: ... We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this or ...
DOI:: 10.1007/BF00313799
PubMed:: 8001165
: http://dx.doi.org/10.1007/BF00313799

18. A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis

Author:: Bender, J.; Fink, G.R.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1998 v.95 no.10 pp. 5655-5660
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; transcription factors; messenger RNA; oxo-acid-lyases; loci; structural genes; open reading frames; regulator genes; amino acid sequences; gene expression; histochemistry; mutants; reporter genes; promoter regions; beta-glucuronidase; Show all 16 Subjects
Abstract:: ... In Arabidopsis thaliana, tryptophan pathway genes are induced in response to starvation, wounding, and pathogen attack, resulting in increased production of tryptophan and secondary metabolites important for development and defense. The Arabidopsis tryptophan pathway therefore provides an ideal system for elucidating how environmental stimuli are transduced into changes in plant gene expression. T ...
DOI:: 10.1073/pnas.95.10.5655
PubMed:: 9576939
PubMed Central:: PMC20434
: http://dx.doi.org/10.1073/pnas.95.10.5655

19. An abscisic acid-induced protein kinase, PKABA1, mediates abscisic acid-suppressed gene expression in barley aleurone layers

Author:: Gomez-Cadenas, A.; Verhey, S.D.; Holappa, L.D.; Shen, Q.; Ho, T.H.D.; Walker-Simmons, M.K.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.4 pp. 1767-1772
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Hordeum vulgare; aleurone layer; gene expression; abscisic acid; messenger RNA; alpha-amylase; cysteine proteinases; plant proteins; reporter genes; beta-glucuronidase; transgenic plants; promoter regions; histochemistry; Show all 14 Subjects
Abstract:: ... The phytohormone abscisic acid (ABA) induces genes-encoding proteins involved in desiccation tolerance and dormancy in seeds, but ABA also suppresses gibberellin (GA)-responsive genes encoding hydrolytic enzymes essential for postgermination growth. A unique serine/threonine protein kinase, PKABA1 mRNA, up-regulated by ABA in seeds, has been identified. In this report, the effect of PKABA1 on the ...
DOI:: 10.1073/pnas.96.4.1767
PubMed:: 9990099
PubMed Central:: PMC15589
: http://dx.doi.org/10.1073/pnas.96.4.1767

20. An alternative intronic promoter of the Bombyx A3 cytoplasmic actin gene exhibits a high level of transcriptional activity in mammalian cells

Author:: Fatyol, K.; Illes, K.; Szalay, A.A.
Source:: MGG 1999 v.261 no.2 pp. 337-345
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; hamsters; chickens; monkeys; beta-galactosidase; transfection; gene expression; mice; Bombyx mori; reporter genes; actin; cell lines; introns; promoter regions; Show all 14 Subjects
DOI:: 10.1007/s004380050974
PubMed:: 10102369
: http://dx.doi.org/10.1007/s004380050974

21. Analysis of the promoter activity of the Taka-amylase gene and the phosphoglycerate kinase gene in a shoyu-koji mold Aspergillus oryzae KBN616

Author:: Kimura, T.; Makino, T.; Aburatani, T.; Karita, S.; Sakka, Z.; Ohmiya, K.
Source:: Food science and technology research 2000 v.6 no.1 pp. 44-47
ISSN:: 1344-6606
Subject:: recombinant DNA, etc ; Aspergillus oryzae; genetic transformation; promoter regions; protein synthesis; phosphoglycerate kinase; glucose; nutrient availability; starch; gene expression; reporter genes; alpha-amylase; beta-glucuronidase; Show all 13 Subjects
DOI:: 10.3136/fstr.6.44
: http://dx.doi.org/10.3136/fstr.6.44

22. Analysis of Tox5 gene expression in Gibberella pulicaris strains with different trichothecene production phenotypes

Author:: Hohn, T.M.; Desjardins, A.E.; McCormick, S.P.
Source:: Applied and environmental microbiology 1993 v.59 no.8 pp. 2359
ISSN:: 0099-2240
Subject:: recombinant DNA, etc ; Gibberella pulicaris; gene expression; structural genes; ligases; messenger RNA; alleles; trichothecenes; biosynthesis; reporter genes; beta-galactosidase; genetic transformation; promoter regions; repetitive sequences; transcription (genetics); Show all 15 Subjects
Abstract:: ... The Tox5 gene encodes trichodiene synthase, the first unique enzyme in the trichothecene biosynthetic pathway. In Gibberella pulicaris R-6380, the level of Tox5 mRNA was found to increase 47-fold in early stationary phase. Sequence analysis of the Tox5 promoter regions from geographically distinct strains of G. pullicaris revealed the existence of two Tox5 alleles (Tox5-1 and Tox5-2). All G. pulic ...
Handle:: 10113/24607
PubMed:: 8368827
PubMed Central:: PMC182291

23. An ethylene-responsive enhancer element is involved in the senescence-related expression of the carnation glutathione-S-transferase (GST1) gene

Author:: Itzhaki, H.; Maxson, J.M.; Woodson, W.R.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.19 pp. 8925-8929
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; transcription factors; ethylene; structural genes; glutathione transferase; transcription (genetics); gene expression; binding sites; Dianthus caryophyllus; reporter genes; DNA-binding proteins; promoter regions; beta-glucuronidase; Show all 13 Subjects
Abstract:: ... The increased production of ethylene during carnation petal senescence regulates the transcription of the GST1 gene encoding a subunit of glutathione-S-transferase. We have investigated the molecular basis for this ethylene-responsive transcription by examining the cis elements and trans-acting factors involved in the expression of the GST1 gene. Transient expression assays following delivery of G ...
DOI:: 10.1073/pnas.91.19.8925
PubMed:: 8090746
PubMed Central:: PMC44719
: http://dx.doi.org/10.1073/pnas.91.19.8925

24. An evolutionarily conserved palindrome in the Drosophila Gld promoter directs tissue-specific expression

Author:: Gunaratne, P.; Ross, J.L.; Zhang, Q.; Organ, E.L.; Cavener, D.R.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.7 pp. 2738-2742
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Drosophila melanogaster; alcohol oxidoreductases; reporter genes; beta-galactosidase; gene expression; male genitalia; promoter regions; transcription (genetics); histochemistry; Show all 10 Subjects
Abstract:: ... A conserved palindromic sequence (Gpal) in the promoter region of the Drosophila Gld directs expression of a heterologous reporter gene in transgenic flies to the anterior spiracular glands of third instar larvae and to the ejaculatory bulb of adult males. The Gld gene is normally expressed at high levels in the anterior spiracular glands but is not expressed in the ejaculatory bulb of Drosophila ...
DOI:: 10.1073/pnas.91.7.2738
PubMed:: 8146184
PubMed Central:: PMC43445
: http://dx.doi.org/10.1073/pnas.91.7.2738

25. A novel element in the promoter of the Saccharomyces cerevisiae gene SPS19 enhances ORE-dependent up-regulation in oleic acid and is essential for de-repression

Author:: Gurvitz, A.; Hamilton, B.; Hartig, A.; Ruis, H.; Dawes, I.W.; Rottensteiner, H.
Source:: MGG 1999 v.262 no.3 pp. 481-492
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; transcription factors; peroxisomes; beta-galactosidase; Saccharomyces cerevisiae; oxidoreductases; gene expression; transcription (genetics); reporter genes; oleic acid; promoter regions; Show all 11 Subjects
DOI:: 10.1007/s004380051109
PubMed:: 10589836
: http://dx.doi.org/10.1007/s004380051109

26. A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells

Author:: Kim, K.Y.; Kwon, S.Y.; Lee, H.S.; Hur, Y.; Bang, J.W.; Kwak, S.S.
Source:: Plant molecular biology 2003 v.51 no.6 pp. 831-838
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; sweet potatoes; cell lines; ultraviolet radiation; plant damage; promoter regions; oxidative stress; hydrogen peroxide; Ipomoea batatas; gene expression; Nicotiana tabacum; gene expression regulation; introns; peroxidase; complementary DNA; tobacco; plant stress; transgenic plants; reporter genes; beta-glucuronidase; Show all 20 Subjects
Abstract:: ... A strong oxidative stress-inducible peroxidase (POD) promoter was cloned from sweetpotato (Ipomoea batatas) and characterized in transgenic tobacco plants and cultured cells in terms of environmental stress. A POD genomic clone (referred to as SWPA2) consisted of 1824 bp of sequence upstream of the translation start site, two introns (743 bp and 97 bp), and a 1073 bp coding region. SWPA2 had previ ...
PubMed:: 12777043

27. A nuclear gene, erd1, encoding a chloroplast-targeting Clp protease regulatory subunit homolog is not only induced by water stress but also developmentally up-regulated during senescence in Arabidopsis thaliana

Author:: Nakashima, K.; Kiyosue, T.; Yamaguchi-Shinozaki, K.; Shinozaki, K.
Source:: The plant journal 1997 v.12 no.4 pp. 851-861
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; chloroplast DNA; drought; protein transport; etiolation; promoter regions; water stress; gene expression; trans fatty acids; adenosinetriphosphatase; Arabidopsis thaliana; complementary DNA; protoplasts; synthetic proteins; green fluorescent protein; salinity; transgenic plants; proteinases; reporter genes; abscisic acid; beta-glucuronidase; chloroplasts; Show all 22 Subjects
Abstract:: ... A cDNA, ERD1, isolated from one-hour-dehydrated plants of Arabidopsis thaliana L. encodes a putative protein that is similar to the regulatory ATPase subunit (ClpA) of the Clp protease and contains a putative chloroplast-targeting transit-peptide at the N-terminus. A chimeric gene with the putative plastid-targeting sequence of the erd1 gene fused to the synthetic green-fluorescent protein (sGFP) ...
DOI:: 10.1046/j.1365-313X.1997.12040851.x
: http://dx.doi.org/10.1046/j.1365-313X.1997.12040851.x

28. A peroxidase gene promoter induced by phytopathogens and methyl jasmonate in transgenic plants

Author:: Curtis, M.D.; Rae, A.L.; Rusu, A.G.; Harrison, S.J.; Manners, J.M.
Source:: Molecular plant-microbe interactions 1997 v.10 no.3 pp. 326-338
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; promoter regions; Glomerella cingulata; messenger RNA; transgenic plants; Stylosanthes humilis; gene expression; histochemistry; Nicotiana tabacum; growth retardation; reporter genes; Phytophthora nicotianae var. parasitica; mechanical damage; peroxidase; beta-glucuronidase; leaves; Show all 16 Subjects
Abstract:: ... The expression of two closely related peroxidase iso-genes, Shpx6a and Shpx6b, of the legume Stylosanthes humilis was studied using isogene-specific reverse transcriptase PCR techniques. Results indicated that transcripts of both genes were rapidly induced following inoculation with the fungal pathogen Colletotrichum gloeosporioides, wounding and treatment with the defense regulator methyl jasmona ...
DOI:: 10.1094/MPMI.1997.10.3.326
PubMed:: 9100378
: http://dx.doi.org/10.1094/MPMI.1997.10.3.326

29. Appetite of an epiphyte: quantitative monitoring of bacterial sugar consumption in the phyllosphere

Author:: Leveau, J.H.J.; Lindow, S.E.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2001 v.98 no.6 pp. 3446-3453
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Pantoea agglomerans; genetically modified organisms; reporter genes; Scyphozoa; animal proteins; gene expression; fructose; sucrose; nutrient availability; fluorescence; Phaseolus vulgaris; carbohydrate metabolism; leaves; surfaces; promoter regions; green fluorescent protein; Show all 17 Subjects
DOI:: 10.1073/pnas.061629598
PubMed:: 11248098
PubMed Central:: PMC30673
: http://dx.doi.org/10.1073/pnas.061629598

30. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila

Author:: Morin, X.; Daneman, R.; Zavortink, M.; Chia, W.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2001 v.98 no.26 pp. 15050-15055
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Drosophila melanogaster; reporter genes; animal proteins; Scyphozoa; exons; transposons; loci; gene expression; fluorescence; cytochemistry; mutation; promoter regions; green fluorescent protein; Show all 14 Subjects
Abstract:: ... In Drosophila, enhancer trap strategies allow rapid access to expression patterns, molecular data, and mutations in trapped genes. However, they do not give any information at the protein level, e.g., about the protein subcellular localization. Using the green fluorescent protein (GFP) as a mobile artificial exon carried by a transposable P-element, we have developed a protein trap system. We scre ...
DOI:: 10.1073/pnas.261408198
PubMed:: 11742088
PubMed Central:: PMC64981
: http://dx.doi.org/10.1073/pnas.261408198

31. ARG1 (altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton

Author:: Sedbrook, J.C.; Chen, R.; Masson, P.H.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.3 pp. 1140-1145
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; promoter regions; roots; plant proteins; polypeptides; structural genes; hypocotyls; binding proteins; gene expression; Arabidopsis thaliana; restriction fragment length polymorphism; messenger RNA; amino acid sequences; chromosome mapping; histochemistry; gravitropism; transgenic plants; cotyledons; reporter genes; mutants; beta-glucuronidase; Show all 21 Subjects
Abstract:: ... Gravitropism allows plant organs to direct their growth at a specific angle from the gravity vector promoting upward growth for shoots and downward growth for roots. Little is known about the mechanisms underlying gravitropic signal transduction. We found that mutations in the ARG1 locus of Arabidopsis thaliana after root and hypocotyl gravitropism without affecting phototropism root growth respon ...
DOI:: 10.1073/pnas.96.3.1140
PubMed:: 9927707
PubMed Central:: PMC15364
: http://dx.doi.org/10.1073/pnas.96.3.1140

32. A Saccharomyces cerevisiae mitochondrial DNA fragment activates Reg1p-dependent glucose-repressible transcription in the nucleus

Author:: Santangelo, G.M.; Tornow, J.
Source:: Current genetics 1997 v.32 no.6 pp. 389-398
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; transcription factors; nucleocytoplasmic interaction; mitochondrial DNA; glucose; Saccharomyces cerevisiae; beta-galactosidase; gene expression; transcription (genetics); chromosome mapping; reporter genes; promoter regions; Show all 12 Subjects
DOI:: 10.1007/s002940050293
PubMed:: 9388294
: http://dx.doi.org/10.1007/s002940050293

33. Asparaginase gene expression is regulated in a complex spatial and temporal pattern in nitrogen-sink tissues

Author:: Grant, M.; Bevan, M.W.
Source:: The plant journal 1994 v.5 no.5 pp. 695-704
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; Lupinus angustifolius; Nicotiana tabacum; asparaginase; reporter genes; beta-glucuronidase; gene expression; transgenic plants; leaves; apical meristems; seed development; promoter regions; histochemistry; Show all 13 Subjects
DOI:: 10.1111/j.1365-313X.1994.00695.x
: http://dx.doi.org/10.1111/j.1365-313X.1994.00695.x

34. A system for tissue-specific copper-controllable gene expression in transgenic plants: nodule-specific antisense of asparate aminotransferase-P2

Author:: Mett, V.L.; Podivinsky, E.; Tennant, A.M.; Lochhead, L.P.; Jones, W.T.; Reynolds, P.H.S.
Source:: Transgenic research 1996 v.5 no.2 pp. 105-113
ISSN:: 0962-8819
Subject:: recombinant DNA, etc ; aspartate transaminase; antisense DNA; promoter regions; structural genes; gene expression; Lotus corniculatus; metal ions; transcription factors; copper; root nodules; enzyme activity; histochemistry; asparagine; transgenic plants; plasmid vectors; Saccharomyces cerevisiae; binding sites; DNA-binding proteins; reporter genes; beta-glucuronidase; Show all 21 Subjects
DOI:: 10.1007/BF01969428
: http://dx.doi.org/10.1007/BF01969428

35. A two-reporter gene system for the analysis of bi-directional transcription from the divergent MAL6T-MAL6S promoter in Saccharomyces cerevisiae

Author:: Bell, P.J.L.; Bissinger, P.H.; Evans, R.J.; Dawes, I.W.
Source:: Current genetics 1995 v.28 no.5 pp. 441-446
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Saccharomyces cerevisiae; reporter genes; beta-galactosidase; alpha-galactosidase; gene expression; maltose; glucose; carbohydrate metabolism; plasmid vectors; enzyme activity; promoter regions; transcription (genetics); Show all 13 Subjects
Abstract:: ... Many sets of genes in Saccharomyces cerevisiae are divergently transcribed, but at present there are no vectors generally available for the simultaneous analysis of divergent transcription from these promoters. In the present study MEL1 and lacZ were used to construct a vector capable of measuring the divergent expression initiated by the MAL6T-MAL6S bi-directional promoter. Our observations demon ...
DOI:: 10.1007/BF00310813
PubMed:: 8575017
: http://dx.doi.org/10.1007/BF00310813

36. beta-Phaseolin gene activation is a two-step process: PvALF-facilitated chromatin modification followed by abscisic acid-mediated gene activation

Author:: Li, G.; Bishop, K.J.; Chandrasekharan, M.B.; Hall, T.C.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.12 pp. 7104-7109
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; transcription factors; gene transfer; transcriptional activation; nucleosomes; transgenic plants; DNA conformation; Phaseolus vulgaris; gene expression; phaseolin; transcription (genetics); Nicotiana tabacum; reporter genes; abscisic acid; promoter regions; beta-glucuronidase; leaves; Show all 17 Subjects
Abstract:: ... We have shown previously that a rotationally and translationally positioned nucleosome is responsible for the absence of transcriptional expression from the phaseolin (phas) gene promoter in leaf tissue and that the repressive chromatin structure is disrupted on transcriptional activation during embryogenesis. To investigate how the chromatin structure is modified, we ectopically expressed PvALF, ...
DOI:: 10.1073/pnas.96.12.7104
PubMed:: 10359846
PubMed Central:: PMC22071
: http://dx.doi.org/10.1073/pnas.96.12.7104

37. Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

Author:: Christiansen, S.K.; Knudsen, S.; Giese, H.
Source:: Current genetics 1995 v.29 no.1 pp. 100-102
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; genetic transformation; gene transfer; tubulin; Blumeria graminis f. sp. hordei; gene expression; reporter genes; promoter regions; beta-glucuronidase; Show all 9 Subjects
Abstract:: ... Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces. GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus hetero ...
DOI:: 10.1007/BF00313200
PubMed:: 8595653
: http://dx.doi.org/10.1007/BF00313200

38. Biological sensor for sucrose availability: relative sensitivities of various reporter genes

Author:: Miller, W.G.; Brandl, M.T.; Quinones, B.; Lindow, S.E.
Source:: Applied and environmental microbiology 2001 v.67 no.3 pp. 1308-1317
ISSN:: 0099-2240
Subject:: recombinant DNA, etc ; Phaseolus vulgaris; leaves; surfaces; sucrose; reporter genes; Scyphozoa; animal proteins; beta-galactosidase; Pantoea agglomerans; gene expression; fluorescence; biosensors; chemical constituents of plants; promoter regions; green fluorescent protein; Show all 16 Subjects
Abstract:: ... A set of three sucrose-regulated transcriptional fusions was constructed. Fusions p61RYTIR, p61Rylac, and p61Ryice contain the scrR sucrose repressor gene and the promoterless gfp, lacZ, and inaZ reporter genes, respectively, fused to the scrY promoter from Salmonella enterica serovar Typhimurium. Cells of Erwinia herbicola containing these fusions are induced only in media amended with sucrose, f ...
DOI:: 10.1128/AEM.67.3.1308-1317.2001
PubMed:: 11229926
PubMed Central:: PMC92729
: http://dx.doi.org/10.1128/AEM.67.3.1308-1317.2001

39. Cell-cycle regulation of hydroxyproline-rich glycoprotein HRGPnt3 gene expression during the initiation of lateral root meristems

Author:: Vera, P.; Lamb, C.; Doener, P.W.
Source:: The plant journal 1994 v.6 no.5 pp. 717-727
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; genetic transformation; promoter regions; beta-glucuronidase; transgenic plants; Rhizobium rhizogenes; cell division; gene expression; histochemistry; Nicotiana tabacum; root meristems; reporter genes; glycoproteins; plant proteins; Show all 14 Subjects
DOI:: 10.1046/j.1365-313X.1994.6050717.x
: http://dx.doi.org/10.1046/j.1365-313X.1994.6050717.x

40. Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum

Author:: Thangstad, O.P.; Gilde, B.; Chadchawan, S.; Seem, M.; Husebye, H.; Bradley, D.; Bones, A.M.
Source:: Plant molecular biology 2004 v.54 no.4 pp. 597-611
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; guard cells; transgenic plants; embryo (plant); gene expression; phloem; Nicotiana tabacum; Brassica napus; thioglucosidase; reporter genes; tobacco; enzyme activity; promoter regions; beta-glucuronidase; rapeseed; Show all 16 Subjects
Abstract:: ... A prototypical characteristic of the Brassicaceae is the presence of the myrosinase-glucosinolate system. Myrosinase, the only known S-glycosidase in plants, degrades glucosinolates, thereby initiating the formation of isothiocyanates, nitriles and other reactive products with biological activities. We have used myrosinase gene promoters from Brassica napus and Arabidopsis thaliana fused to the β- ...
PubMed:: 15316292

41. Cellular program controlling the recovery of adipose tissue mass: An in vivo imaging approach

Author:: Birsoy, Kıvanç; Soukas, Alexander; Torrens, Javier; Ceccarini, Giovanni; Montez, Jason; Maffei, Margherita; Cohen, Paul; Fayzikhodjaeva, Gulnorakhon; Viale, Agnes; Socci, Nicholas D.; Friedman, Jeffrey M.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.35 pp. 12985-12990
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; adipocytes; carbohydrate metabolism; leptin; glycogen; luciferase; glycerol; triacylglycerols; weight loss; biochemical pathways; gene expression; acetyl coenzyme A; lipogenesis; adipose tissue; transgenic animals; reporter genes; mice; promoter regions; Show all 18 Subjects
Abstract:: ... The cellular program responsible for the restoration of adipose tissue mass after weight loss is largely uncharacterized. Leptin mRNA levels are highly correlated with adipose tissue mass, and leptin expression can thus be used as a surrogate for changes in the amount of adipose tissue. To further study the responses of adipocytes to changes in weight, we created a transgenic mouse expressing the ...
DOI:: 10.1073/pnas.0805621105
PubMed:: 18753616
PubMed Central:: PMC2526100
: http://dx.doi.org/10.1073/pnas.0805621105

42. Cf gene-dependent induction of a beta-1,3-glucanase promoter in tomato plants infected with Cladosporium fulvum

Author:: Ashfield, T.; Hammond-Kosack, K.E.; Harrison, K.; Jones, J.D.G.
Source:: Molecular plant-microbe interactions 1994 v.7 no.5 pp. 645-657
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; Solanum lycopersicum var. lycopersicum; transgenic plants; beta-glucanase; gene expression; histochemistry; disease resistance; reporter genes; promoter regions; beta-glucuronidase; leaves; Passalora fulva; Show all 12 Subjects
DOI:: 10.1094/MPMI-7-0645
: http://dx.doi.org/10.1094/MPMI-7-0645

43. Characterisation of the glnK-amtB operon and the involvement of AmtB in methylammonium uptake in Azorhizobium caulinodans

Author:: Michel-Reydellet, N.; Desnoues, N.; Zamaroczy, M. de.; Elmerich, C.; Kaminski, P.A.
Source:: MGG 1998 v.258 no.6 pp. 671-677
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; ion transport; cations; bacterial proteins; promoter regions; polypeptides; nitrogen fixation; phenotype; structural genes; nutrient uptake; gene expression; plasma membrane; ammonium compounds; beta-galactosidase; amino acid sequences; transcription (genetics); Rhizobiaceae; nutrient availability; operon; mutants; reporter genes; Show all 21 Subjects
DOI:: 10.1007/s004380050781
PubMed:: 9671036
: http://dx.doi.org/10.1007/s004380050781

44. Characterisation of the Mucor circinelloides regulated promoter gpd1P

Author:: Larsen, Gitte G.; Appel, Karen F.; Wolff, Anne-Mette; Nielsen, Jens; Arnau, José
Source:: Current genetics 2004 v.45 no.4 pp. 225-234
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Mucor; promoter regions; glyceraldehyde-3-phosphate dehydrogenase; mutation; reporter genes; glucose oxidase; Aspergillus niger; gene expression; Show all 9 Subjects
Abstract:: ... The promoter of the Mucor circinelloides gpd1 gene encoding glyceraldehyde-3-phosphate dehydrogenase (gpd1P) was recently cloned and used for the production of recombinant proteins, such as the Aspergillus niger glucose oxidase 1 (GOX). This represents the first example of the application of a strong and regulated promoter from this fungus for recombinant protein production. The original 741-bp gp ...
DOI:: 10.1007/s00294-003-0484-2
PubMed:: 14735314
: http://dx.doi.org/10.1007/s00294-003-0484-2

45. Characterization and properties of heteromeric plant protein complexes that interact with tef cis-acting elements in both RNA polymerase II-dependent promoters and rDNA spacer sequences

Author:: Manevski, A.; Bardet, C.; Tremousaygue, D.; Lescure, B.
Source:: MGG 1999 v.261 no.6 pp. 892-900
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; transcription factors; promoter regions; ribosomal DNA; transgenic plants; binding proteins; gene expression; transcription (genetics); root meristems; reporter genes; intergenic DNA; DNA-directed RNA polymerase; beta-glucuronidase; Show all 14 Subjects
DOI:: 10.1007/s004380051036
PubMed:: 10485279
: http://dx.doi.org/10.1007/s004380051036

46. Characterization of a salicylic acid-insensitive mutant (sai1) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tms2 gene

Author:: Shah, J.; Tsui, F.; Klessig, D.F.
Source:: Molecular plant-microbe interactions 1997 v.10 no.1 pp. 69-78
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; promoter regions; metabolism; loci; transgenic plants; Agrobacterium radiobacter; seedlings; mutation; Pseudomonas syringae pv. tomato; gene expression; salicylic acid; mutants; reporter genes; amide hydrolases; roots; genetic markers; defense mechanisms; Show all 18 Subjects
Abstract:: ... Salicylic acid (SA) plays an important signaling role in the resistance of many plants to pathogen invasion. Increases in endogenous SA levels have been associated with the hypersensitive response as well as systemic acquired resistance (SAR). SA also induces the expression of a subset of the pathogenesis-related (PR) genes. However, relatively little is known about the events occurring subsequent ...
DOI:: 10.1094/MPMI.1997.10.1.69
PubMed:: 9002272
: http://dx.doi.org/10.1094/MPMI.1997.10.1.69

47. Characterization of a 7S globulin-deficient mutant of soybean (Glycine max (L.) Merrill)

Author:: Hayashi, M.; Harada, K.; Fujiwara, T.; Kitamura, K.
Source:: MGG 1998 v.258 no.3 pp. 208-214
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; transcription factors; promoter regions; messenger RNA; Glycine max; beta-conglycinin; cotyledons; transcription (genetics); gene expression; histochemistry; mutants; reporter genes; globulins; beta-glucuronidase; Show all 14 Subjects
DOI:: 10.1007/s004380050724
PubMed:: 9645426
: http://dx.doi.org/10.1007/s004380050724

48. Characterization of a transcriptional activator controlling trichothecene toxin biosynthesis

Author:: Hohn, T.M.; Krishna, R.; Proctor, R.H.
Source:: Fungal genetics and biology 1999 v.26 no.3 pp. 224
ISSN:: 1087-1845
Subject:: recombinant DNA, etc ; Fusarium sporotrichioides; Myrothecium roridum; transcription factors; nucleotide sequences; trichothecenes; biosynthesis; gene expression; reporter genes; beta-glucuronidase; DNA-binding domains; promoter regions; transcription (genetics); site-directed mutagenesis; intergenic DNA; Show all 15 Subjects
Abstract:: ... Trichothecene biosynthetic pathway genes are localized within a gene cluster in Fusarium sporotrichioides and require the zinc-finger containing protein, TRI6, for expression. We show here that TRI6 is able to bind within the promoter regions of nine different pathway genes and that TRI6 binding is involved in pathway gene activation. TRI6 binding occurs at three distinct sites in the TRI5 promote ...
Handle:: 10113/26088
DOI:: 10.1006/fgbi.1999.1122
PubMed:: 10361036
: http://dx.doi.org/10.1006/fgbi.1999.1122

49. Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257

Author:: Gu, J.; Balatti, P.A.; Krishnan, H.B.; Pueppke, S.G.
Source:: Molecular plant-microbe interactions 1997 v.10 no.1 pp. 138-141
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; beta-galactosidase; gene expression; apigenin; reporter genes; Sinorhizobium fredii; promoter regions; Show all 7 Subjects
Abstract:: ... The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the regulatory gene, nodD1. Expression of nolW is constitutive and independent of flavonoids and nodD1. ...
DOI:: 10.1094/MPMI.1997.10.1.138
: http://dx.doi.org/10.1094/MPMI.1997.10.1.138

50. Characterization of the "promoter region" of the enolase-endoding gene enol from the anaerobic fungus Neocallimastix frontalis: sequence and promoter analysis

Author:: Fischer, M.; Durand, R.; Fevre, M.
Source:: Current genetics 1995 v.28 no.1 pp. 80-86
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; genetic transformation; Aspergillus nidulans; rumen fungi; beta-galactosidase; Penicillium roquefortii; gene expression; reporter genes; Neocallimastix; promoter regions; Saprolegnia; hydro-lyases; Show all 12 Subjects
Abstract:: ... The sequence of the Neocallimastix frontalis enolase gene promoter was determined up to 1800 nucleotides 5' to the major transcriptional start point. The base composition of the enolase upstream sequence revealed a very A+T-rich profile (13.5% G+C) leading to many putative hairpin structures. The functional organization of the N. frontalis enolase promoter was investigated by heterologous transien ...
DOI:: 10.1007/BF00311885
: http://dx.doi.org/10.1007/BF00311885

51. Characterization of the ugatA gene of Ustilago maydis, isolated by homology to the gatA gene of Aspergillus nidulans

Author:: Straffon, M.J.; Hynes, M.J.; Davis, M.A.
Source:: Current genetics 1996 v.29 no.4 pp. 360-369
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Aspergillus nidulans; promoter regions; structural genes; transaminases; alanine; gene expression; messenger RNA; nitrogen metabolism; beta-galactosidase; amino acid sequences; transcription (genetics); gamma-aminobutyric acid; Ustilago zeae; genetic transformation; gene transfer; genetic complementation; reporter genes; mutants; Show all 19 Subjects
Abstract:: ... A gene encoding a putative GABA aminotransferase (ugatA) was isolated from the basidiomycete Ustilago maydis via heterologous hybridization to the GABA aminotransferase gene (gatA) of Aspergillus nidulans. The derived amino-acid sequence of ugatA shows strong identity throughout the protein to the GABA aminotransferase enzymes from A. nidulans and Saccharomyces cerevisiae. Northern analysis in U. ...
DOI:: 10.1007/BF02208617
PubMed:: 8598057
: http://dx.doi.org/10.1007/BF02208617

52. Circadian clock- and phytochrome-regulated transcription is conferred by a 78 bp cis-acting domain of the Arabidopsis CAB2 promoter

Author:: Anderson, S.L.; Teakle, G.R.; Martino-Catt, S.J.; Kay, S.A.
Source:: The plant journal 1994 v.6 no.4 pp. 457-470
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; transgenic plants; luciferase; transcription (genetics); gene expression; Nicotiana tabacum; DNA-binding proteins; reporter genes; red light; binding sites; phytochrome; circadian rhythm; promoter regions; Show all 14 Subjects
DOI:: 10.1046/j.1365-313X.1994.6040457.x
PubMed:: 7987408
: http://dx.doi.org/10.1046/j.1365-313X.1994.6040457.x

53. Circadian clock mutants in Arabidopsis identified by luciferase imaging

Author:: Millar, A.J.; Carre, I.A.; Strayer, C.A.; Chua, N.H.; Kay, S.A.
Source:: Science 1995 v.267 no.5201 pp. 1161-1163
ISSN:: 0036-8075
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; image analysis; transgenic plants; luminescence; luciferase; mutation; gene expression; mutants; reporter genes; circadian rhythm; promoter regions; Show all 12 Subjects
Abstract:: ... The cycling bioluminescence of Arabidopsis plants carrying a firefly luciferase fusion construct was used to identify mutant individuals with aberrant cycling patterns. Both long- and short-period mutants were recovered. A semidominant short-period mutation, timing of CAB expression (toc1), was mapped to chromosome 5. The toc1 mutation shortens the period of two distinct circadian rhythms, the exp ...
DOI:: 10.1126/science.7855595
PubMed:: 7855595
: http://dx.doi.org/10.1126/science.7855595

54. cis-Regulatory elements for mesophyll-specific gene expression in the C4 plant Flaveria trinervia, the promoter of the C4 phosphoenolpyruvate carboxylase gene

Author:: Gowik, U.; Burscheidt, J.; Akyildiz, M.; Schlue, U.; Koczor, M.; Streubel, M.; Westhoff, P.
Source:: plant cell 2004 v.16 no.5 pp. 1077-1090
ISSN:: 1040-4651
Subject:: recombinant DNA, etc ; enhancer elements; phosphoenolpyruvate carboxylase; transgenic plants; gene expression; Nicotiana tabacum; reporter genes; Flaveria trinervia; mesophyll; promoter regions; beta-glucuronidase; Show all 11 Subjects
Abstract:: ... C4 photosynthesis depends on the strict compartmentalization of CO2 assimilatory enzymes. cis-regulatory mechanisms are described that ensure mesophyll-specific expression of the gene encoding the C4 isoform of phosphoenolpyruvate carboxylase (ppcA1) of the C4 dicot Flaveria trinervia. To elucidate and understand the anatomy of the C4 ppcA1 promoter, detailed promoter/reporter gene studies were pe ...
DOI:: 10.1105/tpc.019729
PubMed:: 15100398
PubMed Central:: PMC423201
: http://dx.doi.org/10.1105/tpc.019729

55. Comparison of three 3' non-coding regions in Schizosaccharomyces pombe expression vectors: efficiencies of transcription termination and mRNA 3'-end formation

Author:: Patrikakis, M.; Izant, J.G.; Atkins, D.
Source:: Current genetics 1996 v.30 no.2 pp. 151-158
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; genetic transformation; messenger RNA; structural genes; chemical reactions; plasmid vectors; Schizosaccharomyces pombe; beta-galactosidase; transcription (genetics); gene expression; reporter genes; enzyme activity; promoter regions; Show all 13 Subjects
Abstract:: ... Analysis of an established Schizosaccharomyces pombe episomal shuttle vector suggested that inefficient transcription termination was deleterious to plasmid function. We undertook a study to determine if transcription in the presence and absence of 3'-processing within a vector could affect the ability of the plasmid to transform, transcribe and translate the RNA produced. This report provides an ...
DOI:: 10.1007/s002940050114
PubMed:: 8660462
: http://dx.doi.org/10.1007/s002940050114

56. Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides)

Author:: Hu, W.J.; Kawaoka, A.; Tsai, C.J.; Lung, J.; Osakabe, K.; Ebinuma, H.; Chiang, V.L.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1998 v.95 no.9 pp. 5407-5412
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; phenylpropanoids; promoter regions; structural genes; stems; gene expression; Nicotiana tabacum; leaves; messenger RNA; complementary DNA; amino acid sequences; ligases; xylem; lignin; enzyme activity; histochemistry; biosynthesis; transgenic plants; reporter genes; Populus tremuloides; beta-glucuronidase; Show all 21 Subjects
Abstract:: ... 4-Coumarate:CoA ligases (4CLs, EC 6.2.1.12) are a group of enzymes necessary for maintaining a continuous metabolic flux for the biosynthesis of plant phenylpropanoids, such as lignin and flavonoids, that are essential to the survival of plants. So far, various biochemical and molecular studies of plant 4CLs seem to suggest that 4CL isoforms in plants are functionally indistinguishable in mediatin ...
DOI:: 10.1073/pnas.95.9.5407
PubMed:: 9560289
PubMed Central:: PMC20274
: http://dx.doi.org/10.1073/pnas.95.9.5407

57. Constitutive and carbon source-responsive promoter elements are involved in the regulated expression of the Saccharomyces cerevisiae malate synthase gene MLS1

Author:: Caspary, F.; Hartig, A.; Schuller, H.J.
Source:: MGG 1997 v.255 no.6 pp. 619-627
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; Saccharomyces cerevisiae; oxo-acid-lyases; reporter genes; beta-galactosidase; gene expression; glucose; transcription factors; mutants; promoter regions; transcription (genetics); binding sites; site-directed mutagenesis; Show all 13 Subjects
DOI:: 10.1007/s004380050536
PubMed:: 9323366
: http://dx.doi.org/10.1007/s004380050536

58. Continual green-fluorescent protein monitoring of cauliflower mosaic virus 35S promoter activity in nematode-induced feeding cells in Arabidopsis thaliana

Author:: Urwin, P.E.; Moller, S.G.; Lilley, C.J.; McPherson, M.J.; Atkinson, H.J.
Source:: Molecular plant-microbe interactions 1997 v.10 no.3 pp. 394-400
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; infection; Arabidopsis thaliana; Heterodera schachtii; Meloidogyne incognita; roots; gene expression; Cauliflower mosaic virus; animal proteins; Scyphozoa; fluorescence; transgenic plants; reporter genes; chloroplasts; giant cells; promoter regions; Aequorea victoria; Show all 17 Subjects
Abstract:: ... The responsiveness of the cauliflower mosaic virus 35S promoter in feeding sites developed by both sexes of Heterodera schachtii and female Meloidogyne incognita has been studied. The objective was to establish the value of green-fluorescent protein (GFP) as a nondestructive reporter gene system for characterizing promoter activity at nematode feeding sites in vivo. Growth units were devised that ...
DOI:: 10.1094/MPMI.1997.10.3.394
PubMed:: 9100383
: http://dx.doi.org/10.1094/MPMI.1997.10.3.394

59. Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase

Author:: McBride, K.E.; Schaaf, D.J.; Daley, M.; Stalker, D.M.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.15 pp. 7301-7305
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Nicotiana tabacum; bacteriophages; transgenic plants; structural genes; reporter genes; beta-glucuronidase; DNA-directed RNA polymerase; ribulose-bisphosphate carboxylase; amino acid sequences; peptides; gene transfer; gene expression; chloroplasts; genetic transformation; promoter regions; histochemistry; Show all 17 Subjects
Abstract:: ... Phage T7 RNA polymerase has been used extensively in Escherichia coli for high-level expression of selected genes placed under the control of the phage T7 gene 10 promoter. We have constructed an analogous system for use in plastids of higher plants. A T7 RNA polymerase chimeric gene containing a cauliflower mosaic virus 35S promoter and a tobacco ribulose-bisphosphate carboxylase/oxygenase small- ...
DOI:: 10.1073/pnas.91.15.7301
PubMed:: 8041784
PubMed Central:: PMC44387
: http://dx.doi.org/10.1073/pnas.91.15.7301

60. Convergent pathways for lipochitooligosaccharide and auxin signaling in tobacco cells

Author:: Rohrig, H.; Schmidt, J.; Walden, R.; Czaja, I.; Lubenow, H.; Wieneke, U.; Schell, J.; John, M.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1996 v.93 no.23 pp. 13389-13392
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Nicotiana tabacum; Arabidopsis thaliana; reporter genes; beta-glucuronidase; gene expression; oligosaccharides; chitin; kinetin; protoplasts; promoter regions; naphthaleneacetic acid; histochemistry; Show all 13 Subjects
Abstract:: ... Lipochitooligosaccharides (LCOs) are a novel class of plant growth regulators that activate in tobacco protoplasts the expression of AXI1, a gene implicated in auxin signaling. Transient assays with a chimeric P(AXI)-GUS expression plasmid revealed that the N-octadecenoylated monosaccharide GlcN has all structural requirements for a biological active glycolipid, whereas the inactive N-acylated Gal ...
DOI:: 10.1073/pnas.93.23.13389
PubMed:: 11038524
PubMed Central:: PMC24103
: http://dx.doi.org/10.1073/pnas.93.23.13389

61. Cutinase A of Botrytis cinerea is expressed, but not essential, during penetration of gerbera and tomato

Author:: Kan, J.A.L. van; Klooster, J.W. van't.; Wagemakers, C.A.M.; Dees, D.C.T.; Vlugt-Bergmans, C.J.B. van der.
Source:: Molecular plant-microbe interactions 1997 v.10 no.1 pp. 30-38
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; genetic transformation; promoter regions; phenotype; tubulin; Botrytis cinerea; Gerbera; fungal diseases of plants; DNA probes; flowers; gene expression; pathogenicity; histochemistry; mutants; reporter genes; esterases; beta-glucuronidase; infection; Show all 18 Subjects
Abstract:: ... The plant pathogen Botrytis cinerea can infect undamaged plant tissue directly by penetration of the cuticle. This penetration has been suggested to be enzyme-mediated, and an important role for cutinase in the infection process has been proposed. In this study the expression of the cutinase encoding gene cutA of B. cinerea was analyzed using a cutA promoter-GUS reporter gene fusion. Transformants ...
DOI:: 10.1094/MPMI.1997.10.1.30
PubMed:: 9002270
: http://dx.doi.org/10.1094/MPMI.1997.10.1.30

62. Deletion analysis of a nitrite-reductase promoter from spinach in transgenic tobacco

Author:: Neininger, A.; Back, E.; Bichler, J.; Schneiderbauer, A.; Mohr, H.
Source:: Planta 1994 v.194 no.2 pp. 186-192
ISSN:: 0032-0935
Subject:: recombinant DNA, etc ; Spinacia oleracea; Nicotiana tabacum; oxidoreductases; reporter genes; beta-glucuronidase; transgenic plants; gene expression; messenger RNA; nitrates; phytochrome; light; promoter regions; Show all 13 Subjects
Abstract:: ... Deletion analysis of the nitrite-reductase (NiR) promoter from spinach (Spinacia oleracea L.) fused to the beta-glucuronidase (GUS) reporter gene and introduced into tobacco (Nicotiana tabacum L., cv. Coker 176) indicates that basic elements required for light- and nitrate-dependent expression of the reporter are located within the promoter sequence -200/+131 relative to the transcription-initiati ...
DOI:: 10.1007/BF01101677
: http://dx.doi.org/10.1007/BF01101677

63. Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding alpha-glucosidase

Author:: Minetoki, T.; Nunokawa, Y.; Gomi, K.; Kitamoto, K.; Kumagai, C.; Tamura, G.
Source:: Current genetics 1996 v.30 no.5 pp. 432-438
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Aspergillus oryzae; genetic transformation; structural genes; alpha-glucosidase; gene expression; maltose; reporter genes; promoter regions; beta-glucuronidase; Show all 10 Subjects
Abstract:: ... The nucleotide sequence of a 1.5-kb fragment of the promoter region of the Aspergillus oryzae agd A gene encoding alpha-glucosidase was determined. A comparison with the promoter regions of other Aspergillus amylase genes indicated that there are three highly conserved sequences. designated Regions I, II and III, located at -670 nt, -596 nt and -544 nt relative to the start codon, respectively. Th ...
DOI:: 10.1007/s002940050153
PubMed:: 8929396
: http://dx.doi.org/10.1007/s002940050153

64. Developmental expression of tomato heat-shock cognate protein 80

Author:: Koning, A.J.; Rose, R.; Comai, L.
Source:: Plant physiology 1992 v.100 no.2 pp. 801-811
ISSN:: 0032-0889
Subject:: recombinant DNA, etc ; heat shock proteins; genetic transformation; messenger RNA; plant development; structural genes; transgenic plants; amino acid sequences; gene expression; histochemistry; reporter genes; introns; promoter regions; beta-glucuronidase; Solanum lycopersicum var. lycopersicum; Show all 15 Subjects
Abstract:: ... Heat-shock protein 80 (HSP80) is a major heat-shock protein induced in yeast and animals both by heat shock and by specific developmental events. In plants, a heat-shock-induced HSP80 cDNA has been described, although no information concerning developmental regulation of HSP80 genes is available. We have characterized a tomato (Lycopersicon esculentum) gene encoding a typical HSP80 protein. This g ...
DOI:: 10.1104/pp.100.2.801
PubMed:: 16653061
: http://dx.doi.org/10.1104/pp.100.2.801

65. Developmental regulation of a cytosolic ascorbate peroxidase gene from tomato plants

Author:: Gadea, J.; Conejero, V.; Ver, P.
Source:: MGG 1999 v.262 no.2 pp. 212-219
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; roots; promoter regions; stems; peroxidases; gene expression; Nicotiana tabacum; introns; leaves; complementary DNA; amino acid sequences; exons; histochemistry; Solanum lycopersicum var. lycopersicum; seed germination; gene transfer; plant tissues; transgenic plants; reporter genes; beta-glucuronidase; Show all 20 Subjects
DOI:: 10.1007/s004380051077
PubMed:: 10517316
: http://dx.doi.org/10.1007/s004380051077

66. Dicistronic binary vector system—A versatile tool for gene expression studies in cell cultures and plants

Author:: Ali, Zahid; Schumacher, Heinz Martin; Heine-Dobbernack, Elke; El-Banna, Antar; Hafeez, Fauzia Yusuf; Jacobsen, Hans-Jörg; Kiesecker, Heiko
Source:: Journal of biotechnology 2010 v.145 no.1 pp. 9-16
ISSN:: 0168-1656
Subject:: recombinant DNA, etc ; genetic transformation; salt tolerance; peas; transgenic plants; Agrobacterium radiobacter; luciferase; plasmid vectors; gene expression; antiporters; Nicotiana tabacum; sodium; reporter genes; Pisum sativum; promoter regions; beta-glucuronidase; tobacco; Show all 17 Subjects
Abstract:: ... Dicistronic binary vector constructs based on pGreenII vectors for Agrobacterium mediated gene transfer alleviate the translational expression monitoring of a target gene in plants. The functionality of the transformation vectors was proven by marker gene constructs containing a mannopine synthase promoter (p-MAS) fused to a beta-glucuronidase (gus) gene followed by an internal ribosome entry site ...
DOI:: 10.1016/j.jbiotec.2009.10.002
PubMed:: 19835918
: http://dx.doi.org/10.1016/j.jbiotec.2009.10.002

67. Different circadian oscillators control Ca2+ fluxes and Lhcb gene expression

Author:: Sai, J.; Johnson, C.H.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.20 pp. 11659-11663
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Nicotiana tabacum; reporter genes; luciferase; bioluminescence; gene expression; circadian rhythm; seedlings; callus; transgenic plants; red light; promoter regions; light harvesting complex; inorganic ions; calcium; Show all 15 Subjects
Abstract:: ... Circadian biological clocks control many biological events, but the pathways by which these events are controlled are largely unknown. Based on a model suggesting that cytosolic-free calcium levels control the expression of the Lhcb gene in plants, we tested whether the circadian oscillation of free calcium is responsible for driving the rhythm of Lhcb expression. We found that these rhythms free- ...
DOI:: 10.1073/pnas.96.20.11659
PubMed:: 10500233
PubMed Central:: PMC18090
: http://dx.doi.org/10.1073/pnas.96.20.11659

68. Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen

Author:: Eady, C.; Lindsey, K.; Twell, D.
Source:: The plant journal 1994 v.5 no.4 pp. 543-550
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; Solanum lycopersicum var. lycopersicum; Nicotiana tabacum; Arabidopsis thaliana; pollen; reporter genes; beta-glucuronidase; gene expression; transgenic plants; gametogenesis; promoter regions; Show all 11 Subjects
DOI:: 10.1046/j.1365-313X.1994.5040543.x
: http://dx.doi.org/10.1046/j.1365-313X.1994.5040543.x

69. Differential activation of two ACC oxidase gene promoters from melon during plant development and in response to pathogen attack

Author:: Lasserre, E.; Godard, F.; Bouquin, T.; Hernandez, J.A.; Pech, J.C.; Roby, D.; Balague, C.
Source:: MGG 1997 v.256 no.3 pp. 211-222
ISSN:: 0026-8925
Subject:: recombinant DNA, etc ; ethylene; Ralstonia solanacearum; 2,4-D; promoter regions; gene expression; Nicotiana tabacum; mechanical damage; Pseudomonadaceae; oxygenases; infection; genetic recombination; copper sulfate; defense mechanisms; plant development; Cucumis melo; abiotic stress; histochemistry; transgenic plants; reporter genes; abscisic acid; beta-glucuronidase; Show all 22 Subjects
DOI:: 10.1007/s004380050563
PubMed:: 9393445
: http://dx.doi.org/10.1007/s004380050563

70. Differential expression of the Sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants

Author:: Szczyglowski, K.; Potter, T.; Stoltzfus, J.; Fujimoto, S.Y.; Bruijn, F.J. de.
Source:: Plant molecular biology 1996 v.31 no.4 pp. 931-935
ISSN:: 0167-4412
Subject:: recombinant DNA, etc ; gene transfer; promoter regions; leghemoglobin; transgenic plants; gene expression; histochemistry; Nicotiana tabacum; root meristems; reporter genes; site-directed mutagenesis; Lotus corniculatus; Sesbania rostrata; beta-glucuronidase; Show all 14 Subjects
Abstract:: ... The involvement of the Sesbania rostrata glb3 gene promoter NICE (nodule-infected cell expression) element in root-enhanced expression of 5'-Srglb3-uidA-3'nos chimeric gene was investigated in transgenic Nicotiana tabacum plants. The full-length wild-type Srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. The expression pattern of nine selected Srglb3 promoter ...
DOI:: 10.1007/BF00019482
PubMed:: 8806425
: http://dx.doi.org/10.1007/BF00019482

71. Different Levels of Ovine Interferon-tau Gene Expressions Are Regulated Through the Short Promoter Region Including Ets-2 Binding Site

Author:: Matsuda-Minehata, Fuko; Katsumura, Momoko; Kijima, Sho; Christenson, Ronald K.; Imakawa, Kazuhiko
Source:: Molecular reproduction and development 2005 v.72 no.1 pp. 7
ISSN:: 1098-2795
Subject:: recombinant DNA, etc ; sheep; gene expression; interferons; promoter regions; reporter genes; chloramphenicol acetyltransferase; binding sites; transcription factors; Show all 9 Subjects
Abstract:: ... Regulation of interferon-tau (IFN tau) production, a conceptus secretory protein implicated in the process of maternal recognition of pregnancy, has not been fully elucidated. Among more than 10 ovine IFN tau (oIFN tau) gene sequences characterized, approximately 75% of oIFN tau transcripts expressed in utero is derived from oIFN tau-o10 gene and amounts of transcripts from other oIFN tau genes su ...
Handle:: 10113/43652
DOI:: 10.1002/mrd.20329
PubMed:: 15971268
: http://dx.doi.org/10.1002/mrd.20329

72. Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1

Author:: Rosenkrantz, M.; Kell, C.S.; Pennell, E.A.; Webster, M.; Devenish, L.J.
Source:: Current genetics 1994 v.25 no.3 pp. 185-195
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Saccharomyces cerevisiae; structural genes; oxo-acid-lyases; reporter genes; beta-galactosidase; gene expression; glucose; glutamic acid; promoter regions; transcription (genetics); Show all 11 Subjects
Abstract:: ... The yeast CIT1 (mitochondrial citrate synthase) gene is subject to glucose repression and is further repressed by glucose plus glutamate. Based on deletion analysis of a CIT1-lacZ gene fusion. DNA sequences between -548 and -273 are required for full expression of CIT1. The region of transcription initiation and the putative TATA element are located at -150 to -100 and -195 respectively. A restric ...
DOI:: 10.1007/BF00357161
PubMed:: 7923403
: http://dx.doi.org/10.1007/BF00357161

73. Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction

Author:: Mandon, C.A.; Diaz-Latoud, C.; Arrigo, A.P.; Blum, L.J.
Source:: Journal of biotechnology 2006 v.124 no.2 pp. 392-402
ISSN:: 0168-1656
Subject:: recombinant DNA, etc ; thiram; fungicide residues; cadmium; toxicity testing; bioassays; gene expression; reporter genes; luciferase; green fluorescent protein; bioluminescence; fluorescence; promoter regions; Drosophila melanogaster; liver neoplasms; heat shock proteins; uterine cervical neoplasms; cell lines; humans; Show all 19 Subjects
DOI:: 10.1016/j.jbiotec.2006.01.004
: http://dx.doi.org/10.1016/j.jbiotec.2006.01.004

74. DNA methylation and expression of NPT II in transgenic petunias and progeny

Author:: Ulian, E.C.; Magill, J.M.; Magill, C.W.; Smith, R.H.
Source:: Theoretical and applied genetics 1996 v.92 no.8 pp. 976-981
ISSN:: 0040-5752
Subject:: recombinant DNA, etc ; gene transfer; DNA methylation; kanamycin; Petunia hybrida; transgenic plants; gene expression; phosphotransferases (phosphomutases); inheritance (genetics); reporter genes; progeny; enzyme activity; promoter regions; drug resistance; Show all 14 Subjects
Abstract:: ... The expression and inheritance of the NPT II (neomycin phosphotransferase II) gene was studied in four transgenic petunia (Petunia hybrida Vilm.) plants and their progeny. The four transgenic plants, each of which had more than one site of insertion, were different from each other in the level of foreign gene expression. Transmission of one or more NPT II alleles to progeny as detected by DNA hybr ...
DOI:: 10.1007/BF00224037
PubMed:: 24166624
: http://dx.doi.org/10.1007/BF00224037

75. DNA transfection in the ecdysteroid-responsive GV1 cell line from the tobacco hornworm, Manduca sexta

Author:: Lan, Q.; Riddiford, L.M.
Source:: In vitro cellular & developmental biology 1997 v.33 no.8 pp. 615-621
ISSN:: 1071-2690
Subject:: recombinant DNA, etc ; heat shock proteins; Manduca sexta; beta-galactosidase; transfection; gene expression; embryo (animal); reporter genes; ecdysterone; cell lines; promoter regions; chloramphenicol acetyltransferase; Show all 12 Subjects
DOI:: 10.1007/s11626-997-0111-5
PubMed:: 9338143
: http://dx.doi.org/10.1007/s11626-997-0111-5

76. Down-regulation of the Rhizobium ntr system in the determinate nodule of Phaseolus vulgaris identifies a specific developmental zone

Author:: Patriarca, E.J.; Tate, R.; Fedorova, E.; Riccio, A.; Defez, R.; Iaccarino, M.
Source:: Molecular plant-microbe interactions 1996 v.9 no.4 pp. 243-251
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; messenger RNA; Rhizobium leguminosarum; immunohistochemistry; open reading frames; Vicia hirsuta; root nodules; beta-galactosidase; Phaseolus vulgaris; gene expression; Rhizobium etli; DNA-binding proteins; reporter genes; cell differentiation; promoter regions; Show all 15 Subjects
Abstract:: ... The Rhizobium NtrBC two-component system, although essential for the expression of genes involved in ammonium assimilation, is not required for nodule development and efficiency. Moreover, nitrogen assimilation was previously found to be absent in nitrogen-fixing bacteroids. We speculate that this switch-off in nitrogen assimilation may be mediated by the inactivation (dephosphorylation) of the Nt ...
DOI:: 10.1094/MPMI-9-0243
: http://dx.doi.org/10.1094/MPMI-9-0243

77. Ecdysone agonist inducible transcription in transgenic tobacco plants

Author:: Martinez, A.; Sparks, C.; Hart, C.A.; Thompson, J.; Jepson, I.
Source:: The plant journal 1999 v.19 no.1 pp. 97-106
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; Nicotiana tabacum; transgenic plants; DNA-binding domains; transcriptional activation; reporter genes; beta-glucuronidase; gene expression; enzyme activity; tebufenozide; protoplasts; hormone receptors; transcription (genetics); promoter regions; Show all 14 Subjects
Abstract:: ... A novel chemical-induced gene regulatory system for plants consisting of two molecular components is described. The first, or regulatory, cassette comprises a chimeric receptor composed of the hinge and ligand binding domains of the Heliothis virescens ecdysone receptor and the transactivation domain of the Herpes simplex VP16 protein fused to the DNA binding domain and transactivation of a mammal ...
DOI:: 10.1046/j.1365-313X.1999.00504.x
PubMed:: 10417731
: http://dx.doi.org/10.1046/j.1365-313X.1999.00504.x

78. Effects of Rhizobium tropici, R. etli, and R. leguminosarum bv. phaseoli on nod gene-inducing flavonoids in root exudate of Phaseolus vulgaris

Author:: Bolanos-Vasquez, M.C.; Werner, D.
Source:: Molecular plant-microbe interactions 1997 v.10 no.3 pp. 339-346
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; Rhizobium leguminosarum; root exudates; beta-galactosidase; Phaseolus vulgaris; gene expression; genistein; coumestrol; reporter genes; daidzein; Rhizobium etli; promoter regions; Show all 12 Subjects
Abstract:: ... Flavonoids play an important role as signal molecules in the early stages of the legume-Rhizobium symbiosis. The flavonoid content in root exudates of individual seedlings of Phaseolus vulgaris cv. Rab39 was determined by a sensitive method that combines absorption onto cellulose acetate filter strips with separation, identification, and quantification of individual compounds by high-performance l ...
DOI:: 10.1094/MPMI.1997.10.3.339
: http://dx.doi.org/10.1094/MPMI.1997.10.3.339

79. Enhancing leptin response by preventing SH2-containing phosphatase 2 interaction with Ob receptor

Author:: Carpenter, L.R.; Farruggella, T.J.; Symes, A.; Karow, M.L.; Yancopoulos, G.D.; Stahl, N.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1998 v.95 no.11 pp. 6061-6066
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; mice; hormones; polypeptides; hormone receptors; tyrosine; phosphorylation; phosphoprotein phosphatase; gene expression; transcription factors; luciferase; reporter genes; transcription (genetics); promoter regions; Show all 14 Subjects
Abstract:: ... Leptin is an adipocyte-derived cytokine that regulates food intake and body weight via interaction with its Ob receptor (ObR). Serum leptin levels are chronically elevated in obese humans, suggesting that obesity may be associated with leptin resistance and the inability to generate an adequate ObR response. Evidence suggests that transcriptional activation of target genes by STAT3 (signal transdu ...
DOI:: 10.1073/pnas.95.11.6061
PubMed:: 9600917
PubMed Central:: PMC27585
: http://dx.doi.org/10.1073/pnas.95.11.6061

80. ENOD12 gene expression as a molecular marker for comparing Rhizobium-dependent and -independent nodulation in alfalfa

Author:: Pichon, M.; Journet, E.P.; Billy, F. de.; Dedieu, A.; Huguet, T.; Truchet, G.; Barker, D.G.
Source:: Molecular plant-microbe interactions 1994 v.7 no.6 pp. 740-747
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; Medicago sativa nothosubsp. varia; promoter regions; nodulins; transgenic plants; Ensifer meliloti; root nodules; gene expression; histochemistry; reporter genes; nodulation; beta-glucuronidase; Show all 12 Subjects
DOI:: 10.1094/MPMI-7-0740
: http://dx.doi.org/10.1094/MPMI-7-0740

81. Environmental regulation of Campylobacter jejuni major outer membrane protein porin expression in Escherichia coli monitored by using green fluorescent protein

Author:: Dedieu, L.; Pages, J.M.; Bolla, J.M.
Source:: Applied and environmental microbiology 2002 v.68 no.9 pp. 4209-4215
ISSN:: 0099-2240
Subject:: recombinant DNA, etc ; Campylobacter jejuni; cell membranes; bacterial proteins; reporter genes; gene expression; animal proteins; fluorescence; Escherichia coli; temperature; pH; acidity; alkalinity; promoter regions; Show all 14 Subjects
Abstract:: ... Porins allow exchanges between bacteria and their environment. In the gram-negative food-borne pathogen Campylobacter jejuni two porins, major outer membrane protein (MOMP) and Omp50, have been identified. MOMP is synthesized at a very high level under laboratory culture conditions, suggesting that its promoter functions very efficiently under these conditions. In Campylobacter samples, we observe ...
DOI:: 10.1128/AEM.68.9.4209-4215.2002
PubMed:: 12200267
PubMed Central:: PMC124132
: http://dx.doi.org/10.1128/AEM.68.9.4209-4215.2002

82. Epithelial and endothelial expression of the green fluorescent protein reporter gene under the control of bovine prion protein (PrP) gene regulatory sequences in transgenic mice

Author:: Lemaire-Vielle, C.; Schulze, T.; Podevin-Dimster, V.; Follet, J.; Bailly, Y.; Blanquet-Grossard, F.; Decavel, J.P.; Heinen, E.; Cesbron, J.Y.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2000 v.97 no.10 pp. 5422-5427
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; animal proteins; Scyphozoa; promoter regions; small intestine; fluorescence; lymphocytes; gene expression; messenger RNA; alternative splicing; peripheral nerves; neurons; cattle; mice; kidneys; keratinocytes; exons; endothelium; medulla oblongata; thymus gland; transgenic animals; reporter genes; cerebellum; Show all 23 Subjects
Abstract:: ... The expression of the cellular form of the prion protein (PrPc) gene is required for prion replication and neuroinvasion in transmissible spongiform encephalopathies. The identification of the cell types expressing PrPc is necessary to understanding how the agent replicates and spreads from peripheral sites to the central nervous system. To determine the nature of the cell types expressing PrPc, a ...
DOI:: 10.1073/pnas.080081197
PubMed:: 10792029
PubMed Central:: PMC25844
: http://dx.doi.org/10.1073/pnas.080081197

83. Evaluation in tobacco of the organ specificity and strength of the rolD promoter, domain A of the 35S promoter and the 35S2 promoter

Author:: Elmayan, T.; Tepfer, M.
Source:: Transgenic research 1995 v.4 no.6 pp. 388-396
ISSN:: 0962-8819
Subject:: recombinant DNA, etc ; Nicotiana tabacum; Rhizobium rhizogenes; reporter genes; beta-glucuronidase; plasmid vectors; genetic transformation; transgenic plants; gene expression; roots; stems; leaves; promoter regions; histochemistry; Show all 14 Subjects
DOI:: 10.1007/BF01973757
PubMed:: 7581519
: http://dx.doi.org/10.1007/BF01973757

84. Evaluation of a viral thymidine kinase gene for suicide selection in transfected mosquito cells

Author:: Mazzacano, C.A.; Fallon, A.M.
Source:: Insect molecular biology 1995 v.4 no.2 pp. 125-134
ISSN:: 0962-1075
Subject:: recombinant DNA, etc ; Aedes albopictus; structural genes; antiviral agents; transfection; gene expression; thymidine kinase; reporter genes; cell lines; promoter regions; chloramphenicol acetyltransferase; genetic markers; Show all 12 Subjects
Abstract:: ... An Aedes albopictus cell line previously shown to be deficient in thymidine kinase activity was transfected with a thymidine kinase gene (tk) from Herpes simplex virus. Survival of the transfected lines in a 'TK+ selective medium' indicated that the viral gene was actively expressed at a level sufficient to rescue the TK-deficient phenotype of the parent line. Unlike the parental cells, TK+ transf ...
DOI:: 10.1111/j.1365-2583.1995.tb00017.x
PubMed:: 7551194
: http://dx.doi.org/10.1111/j.1365-2583.1995.tb00017.x

85. Expression of a flower-specific Myb protein in leaf cells using a viral vector causes ectopic activation of a target promoter

Author:: Sablowski, R.W.M.; Baulcombe, D.C.; Bevan, M.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.15 pp. 6901-6905
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Nicotiana tabacum; transgenic plants; genetic vectors; Potato virus X; Phaseolus vulgaris; phenylalanine; phenylalanine ammonia-lyase; beta-glucuronidase; reporter genes; gene expression; DNA-binding proteins; promoter regions; transcription factors; Show all 14 Subjects
Abstract:: ... The promoter of the bean PAL2 gene (encoding phenylalanine ammonia-lyase; EC 4.3.1.5) is a model for studies of tissue-restricted gene expression in plants. Petal epidermis is one of the tissues in which this promoter is activated in tobacco. Previous work suggested that a major factor establishing the pattern of PAL2 expression in tobacco petals is the tissue distribution of a protein closely rel ...
DOI:: 10.1073/pnas.92.15.6901
PubMed:: 7624340
PubMed Central:: PMC41438
: http://dx.doi.org/10.1073/pnas.92.15.6901

86. Expression of a higher-plant chloroplast psbD promoter in a cyanobacterium (Synechococcus sp. strain PCC7942) reveals a conserved cis-element, designated PGT, that differentially interacts with sequence-specific binding factors during leaf development

Author:: Christopher, D.A.; Shen, Y.; Dudley, P.; Tsinoremas, N.F.
Source:: Current genetics 1999 v.35 no.6 pp. 657-666
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Hordeum vulgare; chloroplast DNA; photosystem II; plant proteins; reporter genes; beta-galactosidase; gene expression; gene transfer; genetic transformation; Synechococcus; transcription factors; leaves; plant development; promoter regions; transcription (genetics); D2 protein; Show all 17 Subjects
DOI:: 10.1007/s002940050465
PubMed:: 10467011
: http://dx.doi.org/10.1007/s002940050465

87. Expression of a plant viral polycistronic mRNA in yeast, Saccharomyces cerevisiae, mediated by a plant virus translational transactivator

Author:: Sha, Y.; Broglio, E.P.; Cannon, J.F.; Schoelz, J.E.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8911-8915
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Cauliflower mosaic virus; Saccharomyces cerevisiae; viral proteins; structural genes; gene expression; messenger RNA; genetic transformation; plasmid vectors; chloramphenicol acetyltransferase; reporter genes; enzyme activity; promoter regions; Show all 13 Subjects
Abstract:: ... We demonstrate that the cauliflower mosaic virus (CaMV) gene TQ product can transactivate the expression of a reporter gene in bakers' yeast, Saccharomyces cerevisiae. The gene P7 coding sequence was placed under the control of the galactose-inducible promoter GAL1, which is present in the yeast shuttle vector pYES2, to create plasmid JS169. We also created a chloramphenicol acetyltransferase (CAT ...
DOI:: 10.1073/pnas.92.19.8911
PubMed:: 7568042
PubMed Central:: PMC41077
: http://dx.doi.org/10.1073/pnas.92.19.8911

88. Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box

Author:: Vincent, O.; Gancedo, J.M.
Source:: Current genetics 1995 v.27 no.4 pp. 387-389
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; structural genes; plasmid vectors; Saccharomyces cerevisiae; beta-galactosidase; gene expression; transcription (genetics); reporter genes; promoter regions; Show all 9 Subjects
Abstract:: ... We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYC1-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block beta-galactosidase expression. It i ...
DOI:: 10.1007/BF00352109
PubMed:: 7614563
: http://dx.doi.org/10.1007/BF00352109

89. Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts

Author:: Schuren, F.H.J.; Wessels, J.G.H.
Source:: Current genetics 1998 v.33 no.2 pp. 151-156
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; genetic transformation; gene transfer; messenger RNA; Schizophyllum commune; beta-galactosidase; transcription (genetics); gene expression; reporter genes; phosphotransferases (kinases); promoter regions; beta-glucuronidase; Show all 12 Subjects
DOI:: 10.1007/s002940050321
PubMed:: 9506904
: http://dx.doi.org/10.1007/s002940050321

90. Expression of the 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Gene Requires Symbiotic Nitrogen-Fixing Regulator Gene nifA2 in Mesorhizobium loti MAFF303099

Author:: Nukui, Noriyuki; Minamisawa, Kiwamu; Ayabe, Shin-Ichi; Aoki, Toshio
Source:: Applied and environmental microbiology 2006 v.72 no.7 pp. 4964-4969
ISSN:: 0099-2240
Subject:: recombinant DNA, etc ; Mesorhizobium loti; regulator genes; transcription factors; gene expression; transcription (genetics); amidine hydrolases; promoter regions; reporter genes; beta-glucuronidase; root nodules; nodulation; Lotus corniculatus var. japonicus; Show all 13 Subjects
Abstract:: ... Many soil bacteria contain 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which degrades ACC, a precursor of the phytohormone ethylene. In order to examine the regulation of the acdS gene encoding ACC deaminase in Mesorhizobium loti MAFF303099 during symbiosis with the host legume Lotus japonicus, we introduced the β-glucuronidase (GUS) gene into acdS so that GUS was expressed under contro ...
DOI:: 10.1128/AEM.02745-05
PubMed:: 16820494
PubMed Central:: PMC1489367
: http://dx.doi.org/10.1128/AEM.02745-05

91. Expression of the arylsulphatase reporter gene under the control of the nit1 promoter in Chlamydomonas reinhardtii

Author:: Ohresser, M.; Matagne, R.F.; Loppes, R.
Source:: Current genetics 1997 v.31 no.3 pp. 264-271
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; Chlamydomonas reinhardtii; nitrate reductase; reporter genes; arylsulfatase; gene expression; enzyme activity; genetic transformation; nutrient availability; promoter regions; transcription (genetics); ammonium compounds; Show all 12 Subjects
Abstract:: ... In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent or the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promote and introduced this chimeric construction (nit1/ars) into wall-less strain of C. reinhardtii. A new and sensitive method ...
DOI:: 10.1007/s002940050204
PubMed:: 9065390
: http://dx.doi.org/10.1007/s002940050204

92. Expression of the ferrioxamine receptor gene of Erwinia amylovora CFBP 1430 during pathogenesis

Author:: Dellagi, A.; Reis, D.; Vian, B.; Expert, D.
Source:: Molecular plant-microbe interactions 1999 v.12 no.5 pp. 463-466
ISSN:: 0894-0282
Subject:: recombinant DNA, etc ; Erwinia amylovora; animal proteins; Scyphozoa; receptors; promoter regions; blight; iron; gene expression; Malus domestica; active transport; pathogenesis; leaves; chromosomes; chelating agents; green fluorescent protein; mutants; reporter genes; disease course; Show all 19 Subjects
Abstract:: ... Mutants of Erwinia amylovora CFBP 1430 lacking a functional high-affinity iron transport system mediated by desferrioxamine are impaired in their ability to initiate fire blight symptoms (A. Dellagi, M.-N. Brisset, J.-P. Paulin, and D. Expert. Mol. Plant-Microbe Interact. 11:734-742, 1998). In this study, a chromosomal transcriptional lacZ fusion was used to analyze the expression in planta of the ...
DOI:: 10.1094/MPMI.1999.12.5.463
PubMed:: 10226380
: http://dx.doi.org/10.1094/MPMI.1999.12.5.463

93. Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines

Author:: Grasela, J.J.; McIntosh, A.H.; Goodman, C.L.; Wilson, L.E.; King, L.A.
Source:: In vitro cellular & developmental biology 2000 v.36 no.3 pp. 205
ISSN:: 1071-2690
Subject:: recombinant DNA, etc ; infection; Nucleopolyhedrovirus; strains; isolation; Autographa californica; reporter genes; animal proteins; Scyphozoa; viral proteins; gene expression; cell lines; Lepidoptera; fluorescence; promoter regions; Show all 15 Subjects
Abstract:: ... A recombinant AcMNPV containing the green fluorescent protein (gfp) gene under the polyhedrin promoter (polh) was used to investigate the expression of the gfp gene as well as the production of recombinant extracellular virus in 14 continuous insect cell lines, including Heliothis virescens (BCIRL-HV-AM1), Helicoverpa zea (BCIRL-HZ-AM1), Anticarsia gemmatalis (BCIRL-AG-AM1), Trichoplusia ni (TN-CL ...
Handle:: 10113/9001
DOI:: 10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
PubMed:: 10777062
: http://dx.doi.org/10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2

94. Factors affecting transient gene expression in protoplasts isolated from very slowly growing embryogenic callus cultures of wheat (Triticum aestivum L.)

Author:: Zaghmout, O.M.F.; Trolinder, N.L.
Source:: Theoretical and applied genetics 1993 v.86 no.6 pp. 721-730
ISSN:: 0040-5752
Subject:: recombinant DNA, etc ; alcohol dehydrogenase; protoplasts; genetic transformation; Tobacco mosaic virus; transgenic plants; enzyme activity; plasmid vectors; gene expression; histochemistry; Triticum aestivum; reporter genes; Cauliflower mosaic virus; Vitis vinifera; embryogenesis; promoter regions; beta-glucuronidase; callus; electroporation; Show all 19 Subjects
Abstract:: ... Protoplasts isolated from embryogenic ('Mustang' and 'Chinese Spring') and non-embryogenic ('Mit') calli of wheat (Triticum aestivum L.) genotypes transiently expressed beta-glucuronidase (GUS) activity when electroporated with a plasmid containing the GUS gene and driven by an enhanced 35S promoter and a TMV leader sequence. Conditions for the maximum expression of GUS activity were: electroporat ...
DOI:: 10.1007/BF00222662
PubMed:: 24193782
: http://dx.doi.org/10.1007/BF00222662

95. Flavonoid-responsive nodY-lacZ expression in three phylogenetically different Bradyrhizobium groups

Author:: Yokoyama, T.
Source:: Canadian journal of microbiology 2008 v.54 no.5 pp. 401-410
ISSN:: 0008-4166
Subject:: recombinant DNA, etc ; Bradyrhizobium elkanii; beta-galactosidase; gene expression; Bradyrhizobium japonicum; reporter genes; flavonoids; gene expression regulation; promoter regions; Show all 9 Subjects
DOI:: 10.1139/w08-021
PubMed:: 18449225
: http://dx.doi.org/10.1139/w08-021

96. Flavonols and fertilization in Petunia hybrida: localization and mode of action during pollen tube growth

Author:: Yistra, B.; Busscher, J.; Franken, J.; Hollman, P.C.H.; Mol, H.J.N.M.; Tunen, A.J. van.
Source:: The plant journal 1994 v.6 no.2 pp. 201-212
ISSN:: 0960-7412
Subject:: recombinant DNA, etc ; ovules; promoter regions; structural genes; Petunia; antisense DNA; transgenic plants; naringenin-chalcone synthase; flavonols; gene expression; stigma; pollen tubes; reporter genes; histochemistry; beta-glucuronidase; Show all 15 Subjects
DOI:: 10.1046/j.1365-313X.1994.6020201.x
: http://dx.doi.org/10.1046/j.1365-313X.1994.6020201.x

97. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants

Author:: Kovtun, Y.; Chiu, W.L.; Tena, G.; Sheen, J.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2000 v.97 no.6 pp. 2940-2945
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; oxidation; hydrogen peroxide; defense mechanisms; enzyme activity; phosphorylation; gene expression; reporter genes; luciferase; protoplasts; transgenic plants; Nicotiana tabacum; cold stress; heat stress; abscisic acid; promoter regions; naphthaleneacetic acid; Show all 18 Subjects
DOI:: 10.1073/pnas.97.6.2940
PubMed:: 10717008
PubMed Central:: PMC16034
: http://dx.doi.org/10.1073/pnas.97.6.2940

98. Functional analysis of upstream activating elements in the promoter of the FBP1 gene from Saccharomyces cerevisiae

Author:: Mesquita, J.F. de; Zaragoza, O.; Gancedo, J.M.
Source:: Current genetics 1998 v.33 no.6 pp. 406-411
ISSN:: 0172-8083
Subject:: recombinant DNA, etc ; transcription factors; galactose; nutrient availability; fructose-bisphosphatase; glucose; glycerol; mutation; Saccharomyces cerevisiae; beta-galactosidase; gene expression; reporter genes; ethanol; promoter regions; Show all 14 Subjects
DOI:: 10.1007/s002940050353
PubMed:: 9644203
: http://dx.doi.org/10.1007/s002940050353

99. Functional dissection of circadian clock- and phytochrome-regulated transcription of the Arabidopsis CAB2 gene

Author:: Anderson, S.L.; Kay, S.A.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.5 pp. 1500-1504
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; Arabidopsis thaliana; transcription factors; luciferase; transcription (genetics); gene expression; Nicotiana tabacum; DNA-binding proteins; reporter genes; binding sites; site-directed mutagenesis; phytochrome; circadian rhythm; promoter regions; Show all 14 Subjects
Abstract:: ... Both the circadian clock and phytochrome regulate expression of the Arabidopsis genes encoding the light-harvesting chlorophyll a/b-binding proteins (CAB genes). Phytochrome activates CAB transcription, and it has been proposed that the circadian clock negatively regulates CAB transcription. The tobacco nuclear proteins CUF-1 (CAB upstream factor 1) and CGF-1 (CAB GATA factor 1) bind the Arabidops ...
DOI:: 10.1073/pnas.92.5.1500
PubMed:: 7878008
PubMed Central:: PMC42547
: http://dx.doi.org/10.1073/pnas.92.5.1500

100. Galactosides in the rhizosphere: utilization by Sinorhizobium meliloti and development of a biosensor

Author:: Bringhurst, R.M.; Cardon, Z.G.; Gage, D.J.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2001 v.98 no.8 pp. 4540-4545
ISSN:: 0027-8424
Subject:: recombinant DNA, etc ; root tips; Trifolium repens; animal proteins; seedlings; grazing; Protozoa; promoter regions; rhizosphere; sugars; fluorescence; gene expression; seeds; Rhizobiaceae; seed germination; green fluorescent protein; Medicago sativa; root exudates; reporter genes; Medicago truncatula; biosensors; Show all 21 Subjects
Abstract:: ... Identifying the types and distributions of organic substrates that support microbial activities around plant roots is essential for a full understanding of plant-microbe interactions and rhizosphere ecology. We have constructed a strain of the soil bacterium Sinorhizobium meliloti containing a gfp gene fused to the melA promoter which is induced on exposure to galactose and galactosides. We used t ...
DOI:: 10.1073/pnas.071375898
PubMed:: 11274355
PubMed Central:: PMC31870
: http://dx.doi.org/10.1073/pnas.071375898

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1. A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer

2. A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

3. A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription

4. A cis-acting element and a trans-acting factor involved in the wound-induced expression of a horseradish peroxidase gene

5. A clock- and light-regulated gene that links the circadian oscillator to LHCB gene expression

6. Activation of basal transcription by a mutation in SIN4, a yeast global repressor, occurs through a mechanism different from activator-mediated transcriptional enhancement

7. Activation of the promoter of the Tnt1 retrotransposon in tomato after inoulation with the fungal pathogen Cladosporium fulvum

8. Activity of constitutive promoters in various species from the Liliaceae

9. Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element

10. Activity of the promoter of the Lhca3.St.1 gene, encoding the potato apoprotein 2 of the light-harvesting complex of Photosystem I, in transgenic potato and tobacco plants

11. A cyanobacterial gene encoding peptidyl-prolyl cis-trans isomerase

12. Adenine methylation at dam sites increases transient gene expression in plant cells

13. A Drosophila gene promoter is subject to glucose repression in yeast cells

14. A heat-inducible Adh gene as a reporter gene for a negative selection in transgenic Arabidopsis

15. A homolog of the mammalian GTPase Rab2 is present in Arabidopsis and is expressed predominantly in pollen grains and seedlings

16. A hormogonium regulating locus, hrmUA, of the cyanobacterium Nostoc punctiforme strain ATCC 29133 and its response to an extract of a symbiotic plant partner Anthoceros punctatus

17. A luciferase expression system for Physarum that facilitates analysis of regulatory elements

18. A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis

19. An abscisic acid-induced protein kinase, PKABA1, mediates abscisic acid-suppressed gene expression in barley aleurone layers

20. An alternative intronic promoter of the Bombyx A3 cytoplasmic actin gene exhibits a high level of transcriptional activity in mammalian cells

21. Analysis of the promoter activity of the Taka-amylase gene and the phosphoglycerate kinase gene in a shoyu-koji mold Aspergillus oryzae KBN616

22. Analysis of Tox5 gene expression in Gibberella pulicaris strains with different trichothecene production phenotypes

23. An ethylene-responsive enhancer element is involved in the senescence-related expression of the carnation glutathione-S-transferase (GST1) gene

24. An evolutionarily conserved palindrome in the Drosophila Gld promoter directs tissue-specific expression

25. A novel element in the promoter of the Saccharomyces cerevisiae gene SPS19 enhances ORE-dependent up-regulation in oleic acid and is essential for de-repression

26. A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells

27. A nuclear gene, erd1, encoding a chloroplast-targeting Clp protease regulatory subunit homolog is not only induced by water stress but also developmentally up-regulated during senescence in Arabidopsis thaliana

28. A peroxidase gene promoter induced by phytopathogens and methyl jasmonate in transgenic plants

29. Appetite of an epiphyte: quantitative monitoring of bacterial sugar consumption in the phyllosphere

30. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila

31. ARG1 (altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton

32. A Saccharomyces cerevisiae mitochondrial DNA fragment activates Reg1p-dependent glucose-repressible transcription in the nucleus

33. Asparaginase gene expression is regulated in a complex spatial and temporal pattern in nitrogen-sink tissues

34. A system for tissue-specific copper-controllable gene expression in transgenic plants: nodule-specific antisense of asparate aminotransferase-P2

35. A two-reporter gene system for the analysis of bi-directional transcription from the divergent MAL6T-MAL6S promoter in Saccharomyces cerevisiae

36. beta-Phaseolin gene activation is a two-step process: PvALF-facilitated chromatin modification followed by abscisic acid-mediated gene activation

37. Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

38. Biological sensor for sucrose availability: relative sensitivities of various reporter genes

39. Cell-cycle regulation of hydroxyproline-rich glycoprotein HRGPnt3 gene expression during the initiation of lateral root meristems

40. Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum

41. Cellular program controlling the recovery of adipose tissue mass: An in vivo imaging approach

42. Cf gene-dependent induction of a beta-1,3-glucanase promoter in tomato plants infected with Cladosporium fulvum

43. Characterisation of the glnK-amtB operon and the involvement of AmtB in methylammonium uptake in Azorhizobium caulinodans

44. Characterisation of the Mucor circinelloides regulated promoter gpd1P

45. Characterization and properties of heteromeric plant protein complexes that interact with tef cis-acting elements in both RNA polymerase II-dependent promoters and rDNA spacer sequences

46. Characterization of a salicylic acid-insensitive mutant (sai1) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tms2 gene

47. Characterization of a 7S globulin-deficient mutant of soybean (Glycine max (L.) Merrill)

48. Characterization of a transcriptional activator controlling trichothecene toxin biosynthesis

49. Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257

50. Characterization of the "promoter region" of the enolase-endoding gene enol from the anaerobic fungus Neocallimastix frontalis: sequence and promoter analysis

51. Characterization of the ugatA gene of Ustilago maydis, isolated by homology to the gatA gene of Aspergillus nidulans

52. Circadian clock- and phytochrome-regulated transcription is conferred by a 78 bp cis-acting domain of the Arabidopsis CAB2 promoter

53. Circadian clock mutants in Arabidopsis identified by luciferase imaging

54. cis-Regulatory elements for mesophyll-specific gene expression in the C4 plant Flaveria trinervia, the promoter of the C4 phosphoenolpyruvate carboxylase gene

55. Comparison of three 3' non-coding regions in Schizosaccharomyces pombe expression vectors: efficiencies of transcription termination and mRNA 3'-end formation

56. Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides)

57. Constitutive and carbon source-responsive promoter elements are involved in the regulated expression of the Saccharomyces cerevisiae malate synthase gene MLS1

58. Continual green-fluorescent protein monitoring of cauliflower mosaic virus 35S promoter activity in nematode-induced feeding cells in Arabidopsis thaliana

59. Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase

60. Convergent pathways for lipochitooligosaccharide and auxin signaling in tobacco cells

61. Cutinase A of Botrytis cinerea is expressed, but not essential, during penetration of gerbera and tomato

62. Deletion analysis of a nitrite-reductase promoter from spinach in transgenic tobacco

63. Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding alpha-glucosidase

64. Developmental expression of tomato heat-shock cognate protein 80

65. Developmental regulation of a cytosolic ascorbate peroxidase gene from tomato plants

66. Dicistronic binary vector system—A versatile tool for gene expression studies in cell cultures and plants

67. Different circadian oscillators control Ca2+ fluxes and Lhcb gene expression

68. Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen

69. Differential activation of two ACC oxidase gene promoters from melon during plant development and in response to pathogen attack

70. Differential expression of the Sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants