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1. A novel Babesia sp. associated with clinical signs of babesiosis in domestic cats in South Africa

Author:
Oosthuizen, Marinda C.,   et al.   ; Bosman, Anna-Mari; Penzhorn, Barend L.; Brayton, Kelly A.; Schoeman, Tanya;     Show all 5 Authors
Source:
Parasites & vectors 2019 v.12 no.1 pp. 138
ISSN:
1756-3305
Subject:
Babesia microti; Chrysocyon brachyurus; DNA; Procyon lotor; animal pathogens; babesiosis; blood; blood sampling; cats; coasts; dogs; feral animals; microscopy; molecular cloning; new species; nucleic acid hybridization; phylogeny; ribosomal RNA; sequence analysis; signs and symptoms (animals and humans); ticks; type collections; Japan; Mozambique; South Africa; United States
Abstract:
... BACKGROUND: Feline babesiosis, sporadically reported from various countries, is of major clinical significance in South Africa, particularly in certain coastal areas. Babesia felis, B. leo, B. lengau and B. microti have been reported from domestic cats in South Africa. Blood specimens from domestic cats (n = 18) showing clinical signs consistent with feline babesiosis and confirmed to harbour Babe ...
DOI:
10.1186/s13071-019-3395-x

2. Development and validation of a multiplex, real-time PCR assay for Babesia rossi and Babesia vogeli

Author:
Oosthuizen, Marinda C.,   et al.   ; Troskie, Milana; de Villiers, Lourens; Leisewitz, Andrew; Quan, Melvyn;     Show all 5 Authors
Source:
Ticks and tick-borne diseases 2019 v.10 no.2 pp. 421-432
ISSN:
1877-959X
Subject:
Anaplasma platys; Babesia canis subsp. rossi; Babesia canis subsp. vogeli; Babesia gibsoni; Ehrlichia canis; Protozoa; babesiosis; cross reaction; dogs; erythrocytes; genes; hemoparasites; infectious diseases; parasitemia; parasitism; quantitative polymerase chain reaction; ribosomal RNA; ticks; Southern Africa
Abstract:
... Canine babesiosis is caused by tick-transmitted intraerythrocytic protozoan parasites occurring worldwide. In southern Africa, babesiosis is caused by Babesia rossi and B. vogeli and is one of the most common and important infectious diseases affecting dogs. There is no reliable, rapid and sensitive method for the detection of these parasites, especially when parasitaemia is low. The aim of this s ...
DOI:
10.1016/j.ttbdis.2018.12.004

3. Molecular detection and phylogenetic analysis of Anaplasma marginale and Anaplasma centrale amongst transhumant cattle in north-eastern Uganda

Author:
Oosthuizen, Marinda C.,   et al.   ; Byaruhanga, Charles; Collins, Nicola E.; Knobel, Darryn L.; Khumalo, Zamantungwa T.H.; Chaisi, Mamohale E.;     Show all 6 Authors
Source:
Ticks and tick-borne diseases 2018 v.9 no.3 pp. 580-588
ISSN:
1877-959X
Subject:
Anaplasma centrale; Anaplasma marginale; Bayesian theory; DNA; amino acid sequences; blood; bovine anaplasmosis; cattle; confidence interval; epidemiological studies; genes; genetic markers; genetic variation; grazing; heat shock proteins; herds; nucleotide sequences; pastoralism; phylogeny; quantitative polymerase chain reaction; regression analysis; ribosomal RNA; sequence alignment; sequence diversity; ticks; wildlife; Uganda
Abstract:
... There is little molecular data from Anaplasma marginale and Anaplasma centrale isolates from cattle in Uganda. Between November 2013 and January 2014, blood was collected from 240 cattle in 20 randomly-selected herds in two districts of the Karamoja Region in north-eastern Uganda. A duplex quantitative real-time polymerase chain reaction (qPCR) assay was used to detect and determine the prevalence ...
DOI:
10.1016/j.ttbdis.2018.01.012

4. Occurrence of tick-borne haemoparasites in cattle in the Mungwi District, Northern Province, Zambia

Author:
Oosthuizen, Marinda C.,   et al.   ; Tembo, Stephen; Collins, Nicola E.; Sibeko-Matjila, Kgomotso P.; Troskie, Milana; Vorster, Ilse; Byaruhanga, Charles;     Show all 7 Authors
Source:
Ticks and tick-borne diseases 2018 v.9 no.3 pp. 707-717
ISSN:
1877-959X
Subject:
Amblyomma variegatum; Anaplasma marginale; Babesia bovis; DNA; Ehrlichia ruminantium; Rhipicephalus appendiculatus; Rhipicephalus decoloratus; Theileria mutans; anaplasmosis; babesiosis; blood; blood sampling; breeds; cattle; disease occurrence; disease outbreaks; disease vectors; genes; grazing; herds; hybridization; mortality; nucleic acid hybridization; odds ratio; pathogens; ribosomal RNA; rivers; theileriosis; ticks; winter; Zambia
Abstract:
... Little is known about the occurrence of haemoparasites in cattle in communal grazing areas of Mungwi District of Northern Province, Zambia. Clinical signs and post mortem lesions are pathognomonic of mixed tick-borne infections especially babesiosis, anaplasmosis and East Coast fever. The main objective of this study was to screen selected communal herds of cattle for tick-borne haemoparasites, an ...
DOI:
10.1016/j.ttbdis.2018.02.004

5. Occurrence of Hepatozoon canis (Adeleorina: Hepatozoidae) and Anaplasma spp. (Rickettsiales: Anaplasmataceae) in black-backed jackals (Canis mesomelas) in South Africa

Author:
Oosthuizen, Marinda C.,   et al.   ; Penzhorn, Barend L.; Netherlands, Edward C.; Cook, Courtney A.; Smit, Nico J.; Vorster, Ilse; Harrison-White, Robert F.;     Show all 7 Authors
Source:
Parasites & vectors 2018 v.11 no.1 pp. 158
ISSN:
1756-3305
Subject:
Anaplasma phagocytophilum; Babesia canis subsp. rossi; Canis mesomelas; Ehrlichia; Hepatozoon canis; bacteria; blood; blood sampling; dogs; genes; genotype; hybridization; nucleic acid hybridization; nucleotide sequences; polymerase chain reaction; ribosomal DNA; ribosomal RNA; risk; sequence analysis; ticks; virulence; South Africa
Abstract:
... BACKGROUND: Domestic dogs are not native to sub-Saharan Africa, which may account for their susceptibility to Babesia rossi, of which endemic black-backed jackals (Canis mesomelas) are natural reservoirs. There is virtually no information on the occurrence of potentially pathogenic haemogregarines (e.g. Hepatozoon canis) or even rickettsial bacteria (e.g. Ehrlichia spp. and Anaplasma spp.) in indi ...
DOI:
10.1186/s13071-018-2714-y
PubMed:
29554933
PubMed Central:
PMC5859442

6. Piroplasms in brown hyaenas (Parahyaena brunnea) and spotted hyaenas (Crocuta crocuta) in Namibia and South Africa are closely related to Babesia lengau

Author:
Oosthuizen, Marinda C.,   et al.   ; Burroughs, Richard E.J.; Penzhorn, Barend L.; Wiesel, Ingrid; Barker, Nancy; Vorster, Ilse;     Show all 6 Authors
Source:
Parasitology research 2017 v.116 no.2 pp. 685-692
ISSN:
0932-0113
Subject:
Acinonyx jubatus; Anaplasma; Babesia; Crocuta crocuta; DNA; Ehrlichia; Hyaena; Theileria; blood; databases; genes; genotype; humans; hybridization; monophyly; nucleic acid hybridization; parasites; polymerase chain reaction; ribosomal RNA; sequence homology; wildlife; Namibia; South Africa; Western United States
Abstract:
... The objective of our study was identification and molecular characterization of piroplasms and rickettsias occurring in brown (Parahyaena brunnea) and spotted hyaenas (Crocuta crocuta) from various localities in Namibia and South Africa. Whole blood (n = 59) and skin (n = 3) specimens from brown (n = 15) and spotted hyaenas (n = 47) were screened for the presence of Babesia, Theileria, Ehrlichia a ...
DOI:
10.1007/s00436-016-5334-5

7. Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Author:
Oosthuizen, Marinda C.,   et al.   ; Chaisi, Mamohale E.; Collins, Nicola E.;     Show all 3 Authors
Source:
Veterinary parasitology 2014 v.204 no.3-4 pp. 87-95
ISSN:
0304-4017
Subject:
DNA; Syncerus caffer; Theileria buffeli; blood sampling; buffaloes; cattle; cattle industry; genes; genotype; internal transcribed spacers; loci; phylogeny; polymerase chain reaction; ribosomal RNA; sequence diversity; yaks; China; India; South Africa
Abstract:
... Theileria buffeli/orientalis is a group of benign and mildly pathogenic species of cattle and buffalo in various parts of the world. In a previous study, we identified T. buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe–iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterise the 18S rR ...
DOI:
10.1016/j.vetpar.2014.06.001

8. Molecular investigation of tick-borne haemoparasite infections among transhumant zebu cattle in Karamoja Region, Uganda

Author:
Oosthuizen, Marinda C.,   et al.   ; Byaruhanga, Charles; Collins, Nicola E.; Knobel, Darryn; Chaisi, Mamohale E.; Vorster, Ilse; Steyn, Helena C.;     Show all 7 Authors
Source:
Veterinary Parasitology: Regional Studies and Reports 2016 v.3-4 pp. 27-35
ISSN:
2405-9390
Subject:
Anaplasma marginale; Babesia bigemina; Ehrlichia ruminantium; Theileria mutans; Theileria parva; blood; cattle; cattle production; confidence interval; genes; hemoparasites; herds; mixed infection; molecular cloning; nucleic acid hybridization; nucleotide sequences; nucleotides; oligonucleotide probes; pathogens; phylogeny; quantitative polymerase chain reaction; ribosomal RNA; sequence diversity; tick-borne diseases; virulence; zebu; Uganda
Abstract:
... Tick-borne diseases (TBDs) are a major constraint to cattle production in pastoral areas in Africa. Although information on tick-borne infections is important to prioritise control approaches, it is limited for transhumant zebu cattle in Karamoja, Uganda. We conducted a study to determine the occurrence and level of tick-borne infections among cattle in Karamoja Region. A total of 240 cattle were ...
DOI:
10.1016/j.vprsr.2016.06.004

9. Sequence variation identified in the 18S rRNA gene of Theileria mutans and Theileria velifera from the African buffalo (Syncerus caffer)

Author:
Oosthuizen, Marinda C.,   et al.   ; Chaisi, Mamohale E.; Collins, Nicola E.; Potgieter, Fred T.;     Show all 4 Authors
Source:
Veterinary parasitology 2013 v.191 no.1-2 pp. 132-137
ISSN:
0304-4017
Subject:
Babesia; Syncerus caffer; Theileria mutans; Theileria parva; buffaloes; cattle; diagnostic techniques; disease reservoirs; gene targeting; genes; hybridization; mixed infection; nucleotide sequences; polymerase chain reaction; ribosomal RNA; South Africa
Abstract:
... The African buffalo (Syncerus caffer) is a natural reservoir host for both pathogenic and non-pathogenic Theileria species. These often occur naturally as mixed infections in buffalo. Although the benign and mildly pathogenic forms do not have any significant economic importance, their presence could complicate the interpretation of diagnostic test results aimed at the specific diagnosis of the pa ...
DOI:
10.1016/j.vetpar.2012.08.005
PubMed:
22940566

10. Babesia lengau associated with cerebral and haemolytic babesiosis in two domestic cats

Author:
Oosthuizen, Marinda C,   et al.   ; Bosman, Anna-Mari; Venter, Estelle H; Steyl, Johan CA; Gous, Tertius A; Penzhorn, Barend L;     Show all 6 Authors
Source:
Parasites & vectors 2013 v.6 no.1 pp. 128
ISSN:
1756-3305
Subject:
Babesia; babesiosis; cats; chronic diseases; hemolytic anemia; humans; models; monophyly; nucleotide sequences; parasites; polymerase chain reaction; ribosomal DNA; ribosomal RNA; sequence homology; temperature; wildlife; South Africa; United States
Abstract:
... BACKGROUND: Although reported sporadically from various countries, feline babesiosis appears to be a significant clinical entity only in South Africa, where Babesia felis is usually incriminated as the causative agent. Babesia lengau, recently described from asymptomatic cheetahs, has now possibly been incriminated as the causative agent in two severe clinical cases in domestic cats. FINDINGS: Bot ...
DOI:
10.1186/1756-3305-6-128
PubMed:
23634743
PubMed Central:
PMC3652746

11. In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates

Author:
Oosthuizen, Marinda C.,   et al.   ; Zweygarth, Erich; Cabezas-Cruz, Alejandro; Josemans, Antoinette I.; Matjila, Paul T.; Lis, Katarzyna; Broniszewska, Marzena; Schöl, Heidrun; Ferrolho, Joana; Grubhoffer, Libor; Passos, Lygia M.F.;     Show all 11 Authors
Source:
Ticks and tick-borne diseases 2014 v.5 no.4 pp. 423-431
ISSN:
1877-959X
Subject:
DNA; Ehrlichia canis; adhesins; amino acid sequences; bacteria; cell culture; dog diseases; dogs; ehrlichiosis; genes; genetic databases; genetic variation; glycine (amino acid); glycosylation; isoelectric point; nucleotide sequences; pathogens; phylogeny; polymerase chain reaction; probability analysis; ribosomal RNA; ticks
Abstract:
... Ehrlichia canis, the etiologic agent of canine ehrlichiosis, is an obligate intracytoplasmic Gram-negative tick-borne bacterium belonging to the Anaplasmataceae family. E. canis is distributed worldwide and can cause serious and fatal infections in dogs. Among strains of E. canis, the 16S rRNA gene DNA sequences are highly conserved. Using this gene to genetically differentiate isolates is therefo ...
DOI:
10.1016/j.ttbdis.2014.01.011

12. Identification of Theileria parva and Theileria sp. (buffalo) 18S rRNA gene sequence variants in the African Buffalo (Syncerus caffer) in southern Africa

Author:
Oosthuizen, Marinda C.,   et al.   ; Chaisi, Mamohale E.; Sibeko, Kgomotso P.; Collins, Nicola E.; Potgieter, Fred T.;     Show all 5 Authors
Source:
Veterinary parasitology 2011 v.182 no.2-4 pp. 150-162
ISSN:
0304-4017
Subject:
DNA; DNA primers; Syncerus caffer; Theileria parva; blood sampling; buffaloes; cattle; cattle diseases; disease reservoirs; genes; genotype; hybridization; mixed infection; national parks; nucleotide sequences; parasites; phylogeny; polymerase chain reaction; ranching; ribosomal RNA; theileriosis; South Africa
Abstract:
... Theileria parva is the causative agent of Corridor disease in cattle in South Africa. The African buffalo (Syncerus caffer) is the reservoir host, and, as these animals are important for eco-tourism in South Africa, it is compulsory to test and certify them disease free prior to translocation. A T. parva-specific real-time polymerase chain reaction (PCR) test based on the small subunit ribosomal R ...
DOI:
10.1016/j.vetpar.2011.05.041
PubMed:
21700394

13. Occurrence of Theileria and Babesia species in water buffalo (Bubalus babalis, Linnaeus, 1758) in the Hubei province, South China

Author:
Oosthuizen, Marinda C.,   et al.   ; He, Lan; Feng, Hui-Hui; Zhang, Wen-Jie; Zhang, Qing-Li; Fang, Rui; Wang, Li-Xia; Tu, Pan; Zhou, Yan-Qin; Zhao, Jun-Long;     Show all 10 Authors
Source:
Veterinary parasitology 2012 v.186 no.3-4 pp. 490-496
ISSN:
0304-4017
Subject:
Babesia bigemina; Babesia bovis; Bubalus; Theileria buffeli; buffaloes; nucleotide sequences; phylogeny; ribosomal RNA; sequence analysis; China
Abstract:
... The presence and prevalence of tick-borne haemoparasites in water buffalo from the Hubei province, south China was investigated using the reverse line blot (RLB) hybridization assay and phylogenetic analysis of the parasite 18S rRNA gene. Theileria buffeli (19.1%) was the most frequently found species in all of the locations, followed by Babesia orientalis (8.9%), Babesia bovis (1.0%) and Babesia ...
DOI:
10.1016/j.vetpar.2011.11.021
PubMed:
22154255

14. Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

Author:
Oosthuizen, Marinda C.,   et al.   ; Allsopp, Basil A.; Troskie, Milana; Collins, Nicola E.; Penzhorn, Barend L.;     Show all 5 Authors
Source:
Veterinary parasitology 2009 v.163 no.1-2 pp. 39-46
ISSN:
0304-4017
Subject:
wildlife diseases; babesiosis; Babesia; theileriosis; Theileria; new species; emerging diseases; ribosomal DNA; wild animals; Giraffa camelopardalis; antelopes; disease detection; acute course; nucleotide sequences; pathogen identification; parasitemia; molecular epidemiology; sequence analysis; ribosomal RNA; reverse transcriptase polymerase chain reaction; phylogeny; South Africa
Abstract:
... Blood specimens were received from five cases in which young adult giraffe, from different geographic origins in South Africa, showed sudden onset of disease and subsequently died. Additional specimens from two translocated giraffe, as well as one specimen from a roan antelope, were also included in the study. Blood slides from some of these animals showed the presence of piroplasms. DNA was extra ...
DOI:
10.1016/j.vetpar.2009.03.045
PubMed:
19410370

15. Molecular phylogenetic studies on Theileria spp. isolates (China) based on small subunit ribosomal RNA gene sequences

Author:
Oosthuizen, Marinda C.,   et al.   ; Liu, Qin; Zhou, Yanqin Q.; He, Guosheng S.; Zhou, Danna N.; Zhao, Junlong L.;     Show all 6 Authors
Source:
Tropical animal health and production 2010 v.42 no.1 pp. 109-114
ISSN:
0049-4747
Subject:
Theileria sergenti; buffaloes; cattle; genes; goats; nucleotide sequences; parasites; phylogeny; polymerase chain reaction; ribosomal RNA; sequence analysis; sheep; China
Abstract:
... Six Theileria spp. from cattle, buffalo and black goat were identified in the Hubei province of China. In order to study the taxonomic status of these parasites, phylogenetic analysis of 18S rRNA genes were carried out. The 18S rRNA genes from each isolate were amplified by the polymerase chain reaction and the approximate 1.75 kb products were cloned and sequenced. Phylogenetic analysis of these ...
DOI:
10.1007/s11250-009-9392-x
PubMed:
19557541

16. Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Author:
Oosthuizen, Marinda C.,   et al.   ; Bhoora, Raksha; Franssen, Linda; Guthrie, Alan J.; Zweygarth, Erich; Penzhorn, Barend L.; Jongejan, Frans; Collins, Nicola E.;     Show all 8 Authors
Source:
Veterinary parasitology 2009 v.159 no.2 pp. 112-120
ISSN:
0304-4017
Subject:
zebras; horses; horse diseases; babesiosis; Theileria equi; Babesia caballi; theileriosis; nucleotide sequences; genes; ribosomal RNA; assays; epidemiological studies; genotype; phylogeny; eukaryotic cells; diagnostic techniques; South Africa
Abstract:
... A molecular epidemiological survey of the protozoal parasites that cause equine piroplasmosis was conducted using samples collected from horses and zebra from different geographical locations in South Africa. A total of 488 samples were tested for the presence of Theileria equi and/or Babesia caballi using the reverse line blot hybridization assay. Ten percent of the samples hybridized to the Thei ...
DOI:
10.1016/j.vetpar.2008.10.004
PubMed:
19019541

17. Development and evaluation of a real-time polymerase chain reaction test for the detection of Theileria parva infections in Cape buffalo (Syncerus caffer) and cattle

Author:
Oosthuizen, Marinda C.,   et al.   ; Sibeko, Kgomotso P.; Collins, Nicola E.; Geysen, Dirk; Rambritch, Natasha E.; Latif, Abdalla A.; Groeneveld, Hennie T.; Potgieter, Frederick T.; Coetzer, Jacobus A.W.;     Show all 9 Authors
Source:
Veterinary parasitology 2008 v.155 no.1-2 pp. 37-48
ISSN:
0304-4017
Subject:
Syncerus caffer; wild animals; wildlife diseases; cattle; cattle diseases; Theileria parva; animal pathogens; theileriosis; disease reservoirs; hosts; polymerase chain reaction; disease detection; disease diagnosis; diagnostic techniques; ribosomal RNA; South Africa
Abstract:
... Corridor disease, caused by the tick-borne protozoan parasite Theileria parva, is a controlled disease in South Africa. The Cape buffalo is the reservoir host and uninfected buffalo have become sought-after by the game industry in South Africa, particularly for introduction into Corridor disease-free areas. A real-time polymerase chain reaction (PCR) test for detection of T. parva DNA in buffalo a ...
DOI:
10.1016/j.vetpar.2008.03.033
PubMed:
18514421