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1. Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs

Author:
Wu, Jiaqiang,   et al.   ; Yu, Jiang; Zhang, Yuyu; Guo, Lihui; Cong, Xiaoyan; Du, Yijun; Li, Jun; Sun, Wenbo; Shi, Jianli; Peng, Jun; Yin, Feifei; Wang, Dapeng; Zhao, Pengwei; Wang, Jinbao;     Show all 14 Authors
Source:
Veterinary microbiology 2012 v.158 no.3-4 pp. 316-321
ISSN:
0378-1135
Subject:
Haemophilus parasuis; Porcine reproductive and respiratory syndrome virus; gene targeting; heart; histopathology; lungs; polymerase chain reaction; serotypes; swine
Abstract:
... This study was aimed at determining the effect of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) on Haemophilus parasuis (HPS) in co-infection. A quantitative real-time PCR targeting infB gene, which is conserved among different HPS serotypes, was developed to improve the accuracy and speed of the detection of HPS. A total of 32 four-week-old conventional pigs wer ...
DOI:
10.1016/j.vetmic.2012.03.001
PubMed:
22460022

2. Construction and immunogenicity of DNA vaccines encoding fusion protein of murine complement C3d-p28 and GP5 gene of porcine reproductive and respiratory syndrome virus

Author:
Wu, Jiaqiang,   et al.   ; Zhang, Deqing; Xia, Qingxiang; Liu, Dong; Wang, Xiaolong; Niu, Zhongxiang;     Show all 6 Authors
Source:
Vaccine 2011 v.29 no.4 pp. 629-635
ISSN:
0264-410X
Subject:
DNA; Porcine reproductive and respiratory syndrome virus; adjuvants; antigens; cell-mediated immunity; complement; enzyme-linked immunosorbent assay; genes; immune response; industry; interferon-gamma; interleukin-4; mice; neutralizing antibodies; recombinant vaccines; swine
Abstract:
... Porcine reproductive and respiratory syndrome virus (PRRSV) has recently caused catastrophic losses in swine industry worldwide. The commercial vaccines only provide a limited protection against PRRSV infection. At present, DNA vaccine is the focus on the new vaccines. The gene fragment (p28) coding for the molecular adjuvants complement protein C3d (mC3d) from BALB/c mouse was cloned and expresse ...
DOI:
10.1016/j.vaccine.2010.11.046

3. Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) with interferon α/γ in immediate and long-lasting protection against HP-PRRSV challenge

Author:
Wu, Jiaqiang,   et al.   ; Du, Yijun; Qi, Jing; Lu, Yu; Yoo, Dongwan; Liu, Xing; Zhang, Xiumei; Li, Jun; Sun, Wenbo; Cong, Xiaoyan; Shi, Jianli; Wang, Jinbao;     Show all 12 Authors
Source:
Virus genes 2012 v.45 no.3 pp. 474-487
ISSN:
0920-8569
Subject:
Porcine reproductive and respiratory syndrome virus; cell proliferation; immune response; interferon-gamma; interleukin-4; pork industry; recombinant vaccines; swine; vaccination; viremia
Abstract:
... Porcine reproductive and respiratory syndrome virus (PRRSV) has become one of the most economically important diseases to the global pork industry. Current vaccination strategies only provide a limited protective efficacy. In this study, a DNA vaccine, pVAX1©-α-γ-GP35, co-expressing GP3 and GP5 of PRRSV with interferon α/γ was constructed, and its immediate and long-lasting protection against high ...
DOI:
10.1007/s11262-012-0790-1
PubMed:
22843314

4. Identification of putative virulence-associated genes among Haemophilus parasuis strains and the virulence difference of different serovars

Author:
Wu, Jiaqiang,   et al.   ; Yu, Jiang; Zhang, Yuyu; Du, Yijun; Peng, Jun; Chen, Lei; Sun, Wenbo; Cong, Xiaoyan; Xu, Shaojian; Shi, Jianli; Li, Jun; Huang, Baohua; Zhu, Xiwang; Wang, Jinbao;     Show all 14 Authors
Source:
Microbial pathogenesis 2014 v.77 pp. 17-23
ISSN:
0882-4010
Subject:
Haemophilus parasuis; Southern blotting; blood; females; genes; heart; mice; nasal cavity; pericardium; polymerase chain reaction; serotypes; suppression subtractive hybridization; swine; virulence; virulent strains
Abstract:
... This study was aimed at determining virulence-associated genes among Haemophilus parasuis (H. parasuis) strains, and supplying for the Kielstein–Rapp–Gabrielson serotyping scheme. The subtractive fragments, obtained through suppression subtractive hybridization and reverse Southern blot hybridization, were found to encode genes representative of 7 different functions. PCR was used to investigate t ...
DOI:
10.1016/j.micpath.2014.10.001

5. Immunopotentiation of four natural adjuvants co-administered with a highly pathogenic porcine reproductive and respiratory syndrome virus glycoprotein 5 subunit

Author:
Wu, Jiaqiang,   et al.   ; Peng, Jun; Yuan, Yanmei; Shen, Si; Niu, Zhongxiang; Du, Yijun; Li, Jun; Yu, Jiang; Wang, Tao; Wang, Jinbao;     Show all 10 Authors
Source:
Virus genes 2016 v.52 no.2 pp. 261-269
ISSN:
0920-8569
Subject:
Astragalus; Porcine reproductive and respiratory syndrome virus; T-lymphocytes; adjuvants; glycoproteins; humoral immunity; immune response; immunoglobulin G; lymphocyte proliferation; neutralizing antibodies; porcine reproductive and respiratory syndrome; propolis; subunit vaccines; swine; viremia
Abstract:
... To improve the efficacy of porcine reproductive and respiratory syndrome virus (PRRSV) subunit vaccine, the immunological enhancement effects of four natural adjuvants were compared, including Astragalus extract, Astragalus/Bacillus production, propolis, and Freund’s adjuvant. The results showed that titers of IgG and neutralizing antibody against highly pathogenic PRRSV (HP-PRRSV) glycoprotein 5 ...
DOI:
10.1007/s11262-016-1299-9

6. Phylogenetic analysis of 32 porcine circovirus type 2 isolates from Shandong, China

Author:
Wu, Jiaqiang,   et al.   ; Shi, Jianli; Xu, Shaojian; Fu, Fang; Cong, Xiaoyan; Yuan, Xiaoyuan; Peng, Zhe; Sun, Wenbo; Du, Yijun; Li, Jun; Huang, Baohua; Wang, Jinbao;     Show all 12 Authors
Source:
Archives of virology 2015 v.160 no.2 pp. 465-468
ISSN:
0304-8608
Subject:
Porcine circovirus-2; amino acid sequences; databases; genotype; glycosylation; nucleotide sequences; phylogeny; postweaning multisystemic wasting syndrome; swine; vaccines; viruses; China
Abstract:
... Porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome (PMWS), can be divided into distinct genotypes. Thirty-two PCV2 isolates obtained made from pigs in Shandong Province between 2005 and 2013. Complete genome sequences were obtained in three replicates for each virus isolate, and the sequences were submitted to the NCBI database. The ORF1-e ...
DOI:
10.1007/s00705-014-2300-3

7. Porcine reproductive and respiratory syndrome virus NADC30‐like strain accelerates Streptococcus suis serotype 2 infection in vivo and in vitro

Author:
Wu, Jiaqiang,   et al.   ; Li, Jianda; Wang, Jinbao; Liu, Yueyue; Yang, Jie; Guo, Lihui; Ren, Sufang; Chen, Zhi; Liu, Zhaoshan; Zhang, Yuyu; Qiu, Wenbin; Li, Yubao; Zhang, Shujin; Yu, Jiang;     Show all 14 Authors
Source:
Transboundary and emerging diseases 2019 v.66 no.2 pp. 729-742
ISSN:
1865-1674
Subject:
Porcine reproductive and respiratory syndrome virus; Streptococcus suis; bacterial infections; blood; cytokines; emerging diseases; macrophages; mixed infection; mortality; myocarditis; pandemic; pathogenicity; peritonitis; pneumonia; porcine reproductive and respiratory syndrome; serotypes; swine; China
Abstract:
... Porcine reproductive and respiratory syndrome (PRRS), an economically significant pandemic disease, commonly results in increased impact of bacterial infections, including those by Streptococcus suis (S. suis). In recent years, PRRS virus (PRRSV) NADC30‐like strain has emerged in different regions of China, and coinfected with S. suis and PRRSV has also gradually increased in clinical performance. ...
DOI:
10.1111/tbed.13072

8. Potentiation of Taishan Pinus massoniana pollen polysaccharide on the immune response and protection elicited by a highly pathogenic porcine reproductive and respiratory syndrome virus glycoprotein 5 subunit in pigs

Author:
Wu, Jiaqiang,   et al.   ; Peng, Jun; Yuan, Yanmei; Du, Yijun; Li, Baoquan; Li, Jun; Yu, Jiang; Hu, Liping; Shen, Si; Wang, Jinbao; Zhu, Ruiliang;     Show all 11 Authors
Source:
Molecular and cellular probes 2016 v.30 no.2 pp. 83-92
ISSN:
0890-8508
Subject:
Pinus massoniana; Porcine reproductive and respiratory syndrome virus; adjuvants; cytotoxic T-lymphocytes; enzyme-linked immunosorbent assay; glycoproteins; humoral immunity; immune response; inoculum; lymphocyte proliferation; neutralizing antibodies; pollen; polysaccharides; pork industry; subunit vaccines; swine; viremia
Abstract:
... Porcine reproductive and respiratory syndrome virus (PRRSV) heavily affects the global pork industry. Current available vaccine strategies have inherent drawbacks. In this work, the immune enhancement from Taishan Pinus massoniana pollen polysaccharide (TPPPS) and Freund's adjuvant on the efficacy of a PRRSV subunit vaccine were examined. Titers of specific anti-highly pathogenic PRRSV (HP-PRRSV) ...
DOI:
10.1016/j.mcp.2016.01.008

9. The BALB/c mouse infection model for improving the Haemophilus parasuis serotyping scheme

Author:
Wu, Jiaqiang,   et al.   ; Yu, Jiang; Zhang, Yuyu; Du, Yijun; Li, Jun; Huang, Baohua; Sun, Wenbo; Cong, Xiaoyan; Peng, Jun; Ren, Sufang; Gou, Lihui; Shi, Jianli; Chen, Zhi; Chen, Lei; Li, Jianda; Yang, Jie; Wang, Jinbao;     Show all 17 Authors
Source:
Research in veterinary science 2016 v.109 pp. 166-168
ISSN:
0034-5288
Subject:
Haemophilus parasuis; mice; models; serotypes; statistical analysis; swine; virulence
Abstract:
... The use of BALB/c mouse as an alternative model to study Haemophilus parasuis (HPS) infections was evaluated, supplying the serotyping scheme by comparing the pathogenicity of different serovar HPS in pigs and mice challenge using statistical analysis. Results showed that the pathogenicity of different serovar HPS in mouse was consistent with in pigs, proving that this model is a viable alternativ ...
DOI:
10.1016/j.rvsc.2016.10.011

10. The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-κB and p38 MAPK pathways in porcine alveolar macrophages

Author:
Wu, Jiaqiang,   et al.   ; Yu, Jiang; Liu, Yanyan; Zhang, Yuyu; Zhu, Xiwang; Ren, Sufang; Guo, Lihui; Liu, Xing; Sun, Wenbo; Chen, Zhi; Cong, Xiaoyan; Chen, Lei; Shi, Jianli; Du, Yijun; Li, Jun; Wang, Jinbao;     Show all 16 Authors
Source:
Microbial pathogenesis 2017 v.109 pp. 319-324
ISSN:
0882-4010
Subject:
Porcine reproductive and respiratory syndrome virus; dose response; financial economics; immunosuppression; interleukin-10; macrophages; mitogen-activated protein kinase; nucleocapsid; nucleocapsid proteins; porcine reproductive and respiratory syndrome; signal transduction; swine; transcription factor NF-kappa B; viruses
Abstract:
... Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease, has been constantly causing huge economic losses all over the world. PRRS virus (PRRSV) infection results in immunosuppression and IL-10 up-regulation. The relationship between them is still in dispute. Previous studies demonstrated the protein of PRRSV nucleocapsid (N) protein is able to up-regulate IL-10, yet the ...
DOI:
10.1016/j.micpath.2017.04.036