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- Inda, María Eugenia, et al. Show all 4 Authors
- Journal of molecular biology 2019 v.431 no.5 pp. 1016-1024
- biotechnology; dimerization; engineering; histidine kinase; hydrogen bonding; temperature
- ... To address the mechanism of thermosensing and its implications for molecular engineering, we previously deconstructed the functional components of the bacterial thermosensor DesK, a histidine kinase with a five-span transmembrane domain that detects temperature changes. The system was first simplified by building a sensor that consists of a single chimerical transmembrane segment that retained ful ...
- Inda, María Eugenia, et al. Show all 6 Authors
- Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.9 pp. 3579-3584
- Bacillus subtilis; active sites; bacteria; cytoplasm; hydrophilicity; phospholipids; temperature; unsaturated fatty acids
- ... The thermosensor DesK is a multipass transmembrane histidine-kinase that allows the bacterium Bacillus subtilis to adjust the levels of unsaturated fatty acids required to optimize membrane lipid fluidity. The cytoplasmic catalytic domain of DesK behaves like a kinase at low temperature and like a phosphatase at high temperature. Temperature sensing involves a built-in instability caused by a grou ...
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