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1. Purification of a trypsin inhibitor from Psoralea corylifolia seeds and its influence on developmental physiology of Bactrocera cucurbitae

Author:
Samiksha,; Singh, Drishtant; Kesavan, Anup Kumar; Sohal, Satwinder Kaur
Source:
International journal of biological macromolecules 2019 v.139 pp. 1141-1150
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Cullen corylifolium; Zeugodacus cucurbitae; affinity chromatography; artificial diets; biological control agents; catalase; chymotrypsin; elastase; fruit flies; gene expression regulation; genes; high performance liquid chromatography; ion exchange; larvae; larval development; melons; molecular weight; mortality; nutrition assessment; pH; pests; polyacrylamide gel electrophoresis; pupae; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; seeds; superoxide dismutase; temperature; trypsin;     Show all 30 Subjects
Abstract:
... A trypsin inhibitor was purified from the seeds of Psoralea corylifolia by ion-exchange and affinity chromatography. The purified fractions were subjected to RP- HPLC which resolved into a single peak. SDS-PAGE analysis gave an apparent molecular weight of 18 kDa. P. corylifolia trypsin inhibitor (PCTI) was found to be a competitive inhibitor and was active over a broad temperature (10–100 °C) and ...
DOI:
10.1016/j.ijbiomac.2019.08.063

2. Anionic trypsin from the spleen of albacore tuna (Thunnus alalunga): Purification, biochemical properties and its application for proteolytic degradation of fish muscle

Author:
Poonsin, Tanchanok; Simpson, Benjamin K.; Benjakul, Soottawat; Visessanguan, Wonnop; Yoshida, Asami; Osatomi, Kyoshi; Klomklao, Sappasith
Source:
International journal of biological macromolecules 2019 v.133 pp. 971-979
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Thunnus alalunga; agarose; arginine; chromatography; dietary protein; hydrolysis; molecular weight; muscle protein; muscles; pH; polyacrylamide gel electrophoresis; proteolysis; soybeans; spleen; temperature; trypsin; tuna;     Show all 18 Subjects
Abstract:
... Anionic trypsin from albacore tuna spleen was purified by chromatographic separations on Q-Sepharose, Superdex 75 and Arginine Sepharose 4B. The trypsin migrated as single bands in both SDS-PAGE and native-PAGE. The molecular weight of purified trypsin was estimated to be 30 kDa using SDS-PAGE. The enzyme exhibited maximal activity at pH 9.0 and 55 °C for hydrolysis of Boc-Val-Pro-Arg-MCA. pH and ...
DOI:
10.1016/j.ijbiomac.2019.04.122

3. A ratiometric fluorescence sensor for ultra-sensitive detection of trypsin inhibitor in soybean flour using gold nanocluster@carbon nitride quantum dots

Author:
Hu, Xuetao; Shi, Jiyong; Shi, Yongqiang; Li, Wenting; Arslan, Muhammad; Zhang, Wen; Huang, Xiaowei; Li, Zhihua; Xu, Yiwei; Li, Yanxiao; Zou, Xiaobo
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.15 pp. 3341-3351
ISSN:
1618-2642
Subject:
trypsin inhibitors,   etc   ; bovine serum albumin; carbon nitride; detection limit; fluorescence; gold; heat treatment; hydrolysis; nanocomposites; nanogold; quantum dots; sensors (equipment); soy flour; trypsin;     Show all 14 Subjects
Abstract:
... Gold nanocluster@carbon nitride quantum dot nanocomposites protected by bovine serum albumin (BSA-AuNC@CNQDs) were designed as a ratiometric fluorescence nanosensor for ultra-sensitive detection of trypsin inhibitor (TI). CNQDs were prepared via thermal treatment of carbon nitride powder. BSA-CNQDs acted as templates to synthesize BSA-AuNC@CNQDs with dual-emission peaks at 450 and 650 nm. Trypsin ...
DOI:
10.1007/s00216-019-01806-1

4. A strategy for screening trypsin inhibitors from traditional Chinese medicine based on a monolithic capillary immobilized enzyme reactor coupled with offline liquid chromatography and mass spectrometry

Author:
Lin, Hang; Zhang, Changfa; Lin, Yuanjing; Chang, Yiqun; Crommen, Jacques; Wang, Qiqin; Jiang, Zhengjin; Guo, Jialiang
Source:
Journal of separation science 2019 v.42 no.11 pp. 1980-1989
ISSN:
1615-9306
Subject:
trypsin inhibitors,   etc   ; Fourier transform infrared spectroscopy; Oriental traditional medicine; Scutellaria baicalensis; baicalin; batch systems; binding capacity; chemical bonding; computer simulation; enzyme inhibition; epoxides; immobilized enzymes; liquid chromatography; polymers; scanning electron microscopy; screening; solid phase extraction; tandem mass spectrometry; trypsin;     Show all 19 Subjects
Abstract:
... A novel strategy was successfully developed for screening trypsin inhibitors in traditional Chinese medicines based on monolithic capillary immobilized enzyme reactors combined with liquid chromatography‐tandem mass spectrometry. Organic polymer based monolithic enzyme reactors were firstly prepared by covalently bonding trypsin to a poly(glycidyl methacrylate‐co‐poly (ethylene glycol) diacrylate) ...
DOI:
10.1002/jssc.201900169

5. Macromolecular properties and partial amino acid sequence of a Kunitz-type protease inhibitor from okra (Abelmoschus esculentus) seeds

Author:
Datta, Debparna; Pohlentz, Gottfried; Mondal, Saradamoni; Divya, M Bala; Guruprasad, Lalitha; Mormann, Michael; Swamy, Musti J
Source:
Journal of biosciences 2019 v.44 no.2 pp. 35
ISSN:
0250-5991
Subject:
trypsin inhibitors,   etc   ; Abelmoschus esculentus; Theobroma bicolor; ammonium sulfate; anion exchange chromatography; barley; circular dichroism spectroscopy; disulfide bonds; fluorometry; mass spectrometry; models; okra; polyacrylamide gel electrophoresis; reversed-phase high performance liquid chromatography; seeds; sequence analysis; soybeans; trypsin;     Show all 18 Subjects
Abstract:
... A Kunitz-type protease inhibitor (OPI, okra protease inhibitor) has been purified from okra (Abelmoschus esculentus) seeds by a combination of ammonium sulfate precipitation, anion-exchange chromatography and reverse-phase high-performance liquid chromatography. The protein shows an apparent mass of 21 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing condition. OPI e ...
DOI:
10.1007/s12038-019-9859-5

6. Inhibitory Effects of Plant Trypsin Inhibitors <i>Msti-94</i> and <i>Msti-16</i> on <i>Therioaphis trifolii</i> (Monell) (Homoptera: Aphididae) in Alfalfa

Author:
Zhao, Hailong; Ullah, Hidayat; McNeill, Mark Richard; Du, Guilin; Hao, Kun; Tu, Xiongbing; Zhang, Zehua
Source:
Insects 2019 v.10 no.6
ISSN:
2075-4450
Subject:
trypsin inhibitors,   etc   ; Therioaphis trifolii f. maculata; alfalfa; amino acids; aminopeptidases; artificial diets; bacteria; chymotrypsin; crop yield; gene expression regulation; genes; genetic vectors; isoelectric point; messenger RNA; pests; phenotype; physiological response; quantitative polymerase chain reaction; recombinant proteins; reproduction; reverse transcriptase polymerase chain reaction; stomach; survival rate; transcriptome; trypsin;     Show all 25 Subjects
Abstract:
... The spotted alfalfa aphid (Therioaphis trifolii (Monell)) is a known destructive pest that can significantly reduce alfalfa yields. Two differentially up-regulated alfalfa trypsin inhibitors ‘Msti-94’ and ‘Msti-16’ in transcriptome were verified in terms of their mRNA levels using RT-qPCR. The prokaryotic expression vector was constructed and its biological functions, inclu ...
DOI:
10.3390/insects10060154

7. Purification and characterization of Bowman-Birk and Kunitz isoinhibitors from the seeds of Rhynchosia sublobata (Schumach.) Meikle, a wild relative of pigeonpea

Author:
Mohanraj, Soundappan S.; Gujjarlapudi, Mariyamma; Lokya, Vadthya; Mallikarjuna, Nalini; Dutta-Gupta, Aparna; Padmasree, Kollipara
Source:
Phytochemistry 2019 v.159 pp. 159-171
ISSN:
0031-9422
Subject:
trypsin inhibitors,   etc   ; Achaea janata; Helicoverpa armigera; Rhynchosia; Western blotting; acetates; antibodies; chromatography; chymotrypsin; circular dichroism spectroscopy; denaturation; inhibitory concentration 50; insecticidal properties; matrix-assisted laser desorption-ionization mass spectrometry; pigeon peas; seeds; solubility; spectral analysis; trypsin; two-dimensional gel electrophoresis; wild relatives;     Show all 21 Subjects
Abstract:
... Rhynchosia sublobata, a wild relative of pigeonpea, possesses defensive proteinase/protease inhibitors (PIs). Characterization of trypsin specific PIs (RsPI) separated from seeds by column chromatography using 2-D gel electrophoresis and Edman degradation method identified R. sublobata possessed both Bowman-Birk isoinhibitors (RsBBI) and Kunitz isoinhibitors (RsKI). A quick method was developed to ...
DOI:
10.1016/j.phytochem.2018.12.018

8. The protease-based compensatory mechanism to minimize the effect of dietary Soybean Trypsin Inhibitor in Litopenaeus vannamei

Author:
Rojo-Arreola, Liliana; Choquet, Cyril; Cordova-Murueta, Julio; García-Carreño, Fernando
Source:
Aquaculture 2019 v.500 pp. 18-23
ISSN:
0044-8486
Subject:
trypsin inhibitors,   etc   ; Litopenaeus vannamei; chymotrypsin; digestion; enzyme activity; feed formulation; genes; ingestion; ingredients; messenger RNA; metalloproteinases; midgut; phenotypic plasticity; proteolysis; shrimp; soybean meal; soybeans; transcription (genetics); trypsin;     Show all 19 Subjects
Abstract:
... Soybean meal is a recurrent ingredient in shrimp feed formulations; although economically convenient, the downside is the antinutritional molecules often present in its active forms. Recently, a midgut gland proteolytic activity adjustment to compensate the effect of increasing concentrations of soybean trypsin inhibitor (SBTI) delivered in shrimp feed was found in Litopenaeus vannamei; which prom ...
DOI:
10.1016/j.aquaculture.2018.10.002

9. Discovery and Characterization of Cyclic and Acyclic Trypsin Inhibitors from Momordica dioica

Author:
Du, Junqiao; Chan, Lai Yue; Poth, Aaron G.; Craik, David J.
Source:
Journal of natural products 2019 v.82 no.2 pp. 293-300
ISSN:
1520-6025
Subject:
trypsin inhibitors,   etc   ; Momordica cochinchinensis; Momordica dioica; Momordica subangulata; blood serum; carboxylic acids; drug design; epitopes; fruits; genes; humans; insects; neoplasms; peptides; pyrrolidones; seeds; sequence diversity; therapeutics; toxicity; trypsin;     Show all 20 Subjects
Abstract:
... Momordica trypsin inhibitors (TIs) such as those isolated from the seeds of the gấc fruit, Momordica cochinchinensis (MCoTI-I and MCoTI-II), are widely used as scaffolds for drug design studies. To more effectively exploit these molecules in the development of therapeutics, there is a need for wider discovery of the natural sequence diversity among TIs from other species in the Momordica subfami ...
DOI:
10.1021/acs.jnatprod.8b00716

10. Potential Anticoagulant Activity of Trypsin Inhibitor Purified from an Isolated Marine Bacterium Oceanimonas Sp. BPMS22 and its Kinetics

Author:
Harish, B. S.; Uppuluri, Kiran Babu
Source:
Marine biotechnology 2018 v.20 no.6 pp. 780-791
ISSN:
1436-2228
Subject:
trypsin inhibitors,   etc   ; Oceanimonas; affinity chromatography; anion exchange; anticoagulant activity; aquatic bacteria; bacterial growth; blood sampling; enzyme activity; homeostasis; humans; marine sediments; molecular weight; pH; salt concentration; salt tolerance; temperature; trypsin;     Show all 18 Subjects
Abstract:
... Protease inhibitors control major biological protease activities to maintain physiological homeostasis. Marine bacteria isolated from oligotrophic conditions could be taxonomically distinct, metabolically unique, and offers a wide variety of biochemicals. In the present investigation, marine sediments were screened for the potential bacteria that can produce trypsin inhibitors. A moderate halotole ...
DOI:
10.1007/s10126-018-9848-y

11. Viscera of Labeo rohita: A Potential Source of Trypsin for Industrial Application

Author:
Khangembam, Bronson Kumar; Chakrabarti, Rina
Source:
Journal of aquatic food product technology 2018 v.27 no.10 pp. 1078-1092
ISSN:
1547-0636
Subject:
trypsin inhibitors,   etc   ; Labeo rohita; affinity chromatography; aluminum; ammonium sulfate; animal organs; cadmium; calcium chloride; carp; catalytic activity; cobalt; enzyme activity; industrial applications; ion exchange; ions; lithium; magnesium; mercury; molecular weight; pH; potassium; soybeans; temperature; trypsin; zinc;     Show all 25 Subjects
Abstract:
... The serine protease trypsin was isolated and purified from the digestive system of carp Labeo rohita rohu by ammonium sulphate precipitation, ion exchange, and affinity chromatography. The purified enzyme showed high activity between pH 7.0 and 9.0. The activity was maximum at 40°C. Incubation of the purified enzyme with CaCl₂ (2 mM) stabilized the enzyme activity for 8 h. The enzyme showed stabil ...
DOI:
10.1080/10498850.2018.1534300

12. Ultrasensitive detection of trypsin activity and inhibitor screening based on the electron transfer between phosphorescence copper nanocluster and cytochrome c

Author:
Zhang, Shiyu; Chen, Can; Qin, Xiaofei; Zhang, Qianchen; Liu, Jinhua; Zhu, Jixin; Gao, Yongqian; Li, Lin; Huang, Wei
Source:
Talanta 2018 v.189 pp. 92-99
ISSN:
0039-9140
Subject:
trypsin inhibitors,   etc   ; arginine; biomarkers; copper; copper nanoparticles; drugs; electron transfer; fluorescence; fluorescent dyes; heme; hydrolysis; hydrophobic bonding; lysine; phosphorescence; screening; trypsin; urine;     Show all 17 Subjects
Abstract:
... Trypsin, as one of important proteases, is specific for catalyzing the hydrolysis of peptide and ester bonds containing lysine and arginine residues at the C-terminus. The level of trypsin in biological fluids can serve as a reliable and specific diagnostic biomarker for pancreatic function and its pathological changes. Herein, we demonstrate the application of phosphorescent Cu NCs for trypsin de ...
DOI:
10.1016/j.talanta.2018.06.026

13. Structural and functional analysis of miraculin-like protein from Vitis vinifera Proteins and proteomics

Author:
Ohkura, So-ichiro; Hori, Misaho; Saitoh, Kazuki; Okuzawa, Takumi; Okamoto, Ikuko; Furukawa, Nayuta; Shimizu-Ibuka, Akiko
Source:
Biochimica et biophysica acta 2018 v.1866 no.11 pp. 1125-1130
ISSN:
1570-9639
Subject:
trypsin inhibitors,   etc   ; Vitis vinifera; crystal structure; gel chromatography; grapes; proteins; sensory evaluation; sourness; soybeans; sweetness; trypsin;     Show all 11 Subjects
Abstract:
... The so-called miraculin-like proteins (MLPs) are homologous to miraculin, a homodimeric protein with taste-modifying activity that converts sourness into sweetness. The identity between MLPs and miraculin generally ranges from 30% to 55%, and both MLPs and miraculin are categorized into the Kunitz-type soybean trypsin inhibitor (STI) family. MLP from grape (Vitis vinifera; vvMLP) exhibits signific ...
DOI:
10.1016/j.bbapap.2018.08.009

14. A fluorometric sensing method for sensitive detection of trypsin and its inhibitor based on gold nanoclusters and gold nanoparticles

Author:
Wang, Mengke; Su, Dandan; Wang, Guannan; Su, Xingguang
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.26 pp. 6891-6900
ISSN:
1618-2642
Subject:
trypsin inhibitors,   etc   ; electrostatic interactions; energy transfer; fluorescence; fluorometry; humans; models; nanogold; soybeans; trypsin; ultraviolet-visible spectroscopy; urine;     Show all 12 Subjects
Abstract:
... In this work, a facile, label-free, and sensitive fluorometric strategy for detection of trypsin and its inhibitor was established on the basis of the fluorescence resonance energy transfer (FRET) between mercaptoundecanoic acid functionalized gold nanoclusters (AuNCs) and gold nanoparticles (AuNPs) via protamine as a bridge. Protamine can trigger the aggregation of AuNPs and link AuNCs with aggre ...
DOI:
10.1007/s00216-018-1292-3

15. Biochemical characterization a digestive trypsin in the midgut of large cabbage white butterfly, Pieris brassicae L. (Lepidoptera: Pieridae)

Author:
Sharifloo, A.; Zibaee, A.; Jalali Sendi, J.; Talebi Jahroumi, K.
Source:
Bulletin of entomological research 2018 v.108 no.4 pp. 501-509
ISSN:
1475-2670
Subject:
trypsin inhibitors,   etc   ; Pieris brassicae; cellulose; chromatography; insects; larvae; leaves; midgut; molecular weight; nutrition assessment; pH; pests; soybeans; temperature; transgenic plants; trypsin;     Show all 16 Subjects
Abstract:
... A comprehensive study on digestive trypsin was undertaken in the larval midgut of Pieris brassicae L. Results of enzymatic compartmentalization showed a significantly higher activity of crude trypsin in the anterior larval midgut rather than posterior-midgut. Using Diethylaminoethyl cellulose fast flow column chromatography a purified trypsin was obtained by specific activity of 21 U mg⁻¹ protein, ...
DOI:
10.1017/S0007485317001067
PubMed:
29110744

16. Supplementation of broiler diets with high levels of microbial protease and phytase enables partial replacement of commercial soybean meal with raw, full‐fat soybean

Author:
Erdaw, M. M.; Perez‐Maldonado, R. A.; Iji, P. A.
Source:
Journal of animal physiology and animal nutrition 2018 v.102 no.3 pp. 755-768
ISSN:
0931-2439
Subject:
trypsin inhibitors,   etc   ; birds; body weight changes; carboxylic ester hydrolases; digestibility; experimental diets; feed conversion; feed intake; ileum; pancreas; phytases; proteolysis; soybean meal; soybeans; thighs; trypsin; villi;     Show all 17 Subjects
Abstract:
... A 3 × 3 + 1 factorial, involving three levels of protease (0, 15,000 or 30,000 PROT/kg) and three levels of phytase (1,000, 2,000 or 3,000 FYT/kg), was used to evaluate the effect of replacing commercial soybean meal (SBM) with raw, full‐fat soybean (RFSB) at 75 g/kg of diet for broilers. A control diet was used for comparison. Each treatment was replicated six times, with nine birds per replicate ...
DOI:
10.1111/jpn.12876

17. Solar UV‐B radiation and ethylene play a key role in modulating effective defenses against Anticarsia gemmatalis larvae in field‐grown soybean

Author:
Dillon, Francisco M.; Tejedor, M. Daniela; Ilina, Natalia; Chludil, Hugo D.; Mithöfer, Axel; Pagano, Eduardo A.; Zavala, Jorge A.
Source:
Plant, cell and environment 2018 v.41 no.2 pp. 383-394
ISSN:
0140-7791
Subject:
trypsin inhibitors,   etc   ; Anticarsia gemmatalis; correlation; cultivars; ethylene; flavonols; gene expression; genistin; jasmonic acid; larvae; leaves; messenger RNA; pathogenesis; phytophagous insects; salicylic acid; soybeans; trypsin; ultraviolet radiation;     Show all 18 Subjects
Abstract:
... Solar UV‐B radiation has been reported to enhance plant defenses against herbivore insects in many species. However, the mechanism and traits involved in the UV‐B mediated increment of plant resistance are unknown in crops species, such as soybean. Here, we studied defense‐related responses in undamaged and Anticarsia gemmatalis larvae‐damaged leaves of two soybean cultivars grown under attenuated ...
DOI:
10.1111/pce.13104

18. Two trypsin isoforms from albacore tuna (Thunnus alalunga) liver: Purification and physicochemical and biochemical characterization

Author:
Klomklao, Sappasith; Benjakul, Soottawat
Source:
International journal of biological macromolecules 2018 v.107 pp. 1864-1870
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Thunnus alalunga; calcium; gel chromatography; liver; molecular weight; pH; polyacrylamide gel electrophoresis; sodium chloride; soybeans; temperature; trypsin; tuna;     Show all 13 Subjects
Abstract:
... Two trypsins (A and B) from the liver of albacore tuna (Thunnus alalunga) were purified to homogeneity using a series of column chromatographies including Sephacryl S-200, Sephadex G-50 and Diethylaminoethyl-cellulose. Purity was increased to 80.35- and 101.23-fold with approximately 3.1 and 19.2% yield for trypsins A and B, respectively. The molecular weights of trypsins A and B were estimated to ...
DOI:
10.1016/j.ijbiomac.2017.10.059

19. Protective effects of soybean protein and egg white protein on the antibacterial activity of nisin in the presence of trypsin

Author:
Pan, Dan; Zhang, Dong; Hao, Limin; Lin, Songyang; Kang, Qiaozhen; Liu, Xin; Lu, Laizheng; Lu, Jike
Source:
Food chemistry 2018 v.239 pp. 196-200
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; Listeria monocytogenes; Staphylococcus aureus; antibacterial properties; cooked foods; egg albumen; food pathogens; meat processing; meat products; nisin; plate count; protective effect; soy protein; total volatile basic nitrogen; trypsin;     Show all 15 Subjects
Abstract:
... The using of nisin to prevent foodborne pathogens (Staphylococcus aureus and Listeria monocytogenes) from contamination has received broad attentions during meat processing. However, the application of nisin has been limited because its antibacterial activity may be inhibited by trypsin. In this study, the protective effects of soybean protein and egg white protein on antibacterial activity of nis ...
DOI:
10.1016/j.foodchem.2017.06.091

20. Inga vera trypsin inhibitor interferes in the proteolytic activity and nutritional physiology of Ephestia kuehniella larvae

Author:
Bezerra, Cézar da Silva; Oliveira, Carolina Turatti; Macedo, Maria Lígia Rodrigues
Source:
Entomologia experimentalis et applicata 2017 v.165 no.2-3 pp. 109-119
ISSN:
0013-8703
Subject:
trypsin inhibitors,   etc   ; Ephestia kuehniella; Inga vera; bioassays; chymotrypsin; digestion; frass; hydrolysis; ingestion; insect physiology; insects; larvae; midgut; pest control; proteolysis; seeds; trypsin;     Show all 17 Subjects
Abstract:
... Plant peptidase inhibitors provide plants with a defense strategy to inhibit insect digestive enzymes and have been studied as an alternative strategy for pest control as they interfere in normal insect physiology. We evaluated the effects of ingestion of the trypsin inhibitor from Inga vera Willd. (Fabaceae) seeds on the nutritional and digestive physiology of Ephestia kuehniella (Zeller) (Lepido ...
DOI:
10.1111/eea.12605

21. Performance of mechanical co-extraction of Jatropha curcas L. kernels with rapeseed, maize or soybean with regard to oil recovery, press capacity and product quality

Author:
Romuli, Sebastian; Karaj, Shkelqim; Latif, Sajid; Müller, Joachim
Source:
Industrial crops and products 2017 v.104 pp. 81-90
ISSN:
0926-6690
Subject:
trypsin inhibitors,   etc   ; Jatropha curcas; acid value; additives; antinutritional factors; ash content; carbon; corn; corn oil; crude protein; energy requirements; free fatty acids; lipid content; lysine; mixing; nitrogen; pepsin; phytic acid; potassium hydroxide; pressing; product quality; protein sources; rapeseed; response surface methodology; seeds; soybean oil; soybeans; specific energy; temperature; texture; trypsin;     Show all 31 Subjects
Abstract:
... Jatropha curcas L. shelled seeds (kernels) have been gaining attention as protein source due to their high protein content. However, due to the soft texture, screw pressing the kernels resulted in a low performance, which was indicated by high residual oil content in the press cake. In order to overcome the low oil recovery from the extraction process, J. curcas kernels blended with rapeseed, maiz ...
DOI:
10.1016/j.indcrop.2017.03.035

22. Microgramma vacciniifolia (Polypodiaceae) fronds contain a multifunctional lectin with immunomodulatory properties on human cells

Author:
de Siqueira Patriota, Leydianne Leite; Procópio, Thamara Figueiredo; de Santana Brito, Jéssica; Sebag, Virginie; de Oliveira, Ana Patrícia Silva; de Araújo Soares, Ana Karine; Moreira, Leyllane Rafael; de Albuquerque Lima, Thâmarah; Soares, Tatiana; da Silva, Túlio Diego; Paiva, Patrícia Maria Guedes; de Lorena, Virgínia Maria Barros; de Melo, Cristiane Moutinho Lagos; de Albuquerque, Lidiane Pereira; Napoleão, Thiago Henrique
Source:
International journal of biological macromolecules 2017 v.103 pp. 36-46
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Microgramma; RNA-binding proteins; T-lymphocytes; cytotoxicity; fronds; glycoproteins; heat stability; hemagglutination; humans; immunomodulators; interferon-gamma; interleukin-10; interleukin-6; lectins; nitric oxide; pH; sequence homology; trypsin; tumor necrosis factor-alpha;     Show all 20 Subjects
Abstract:
... In this study, we report the purification and characterization of a multifunctional lectin (MvFL) from Microgramma vacciniifolia fronds as well as its immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). MvFL (pI 4.51; 54kDa) is a glycoprotein able to inhibit trypsin activity and that has sequence similarities (32% coverage) with a plant RNA-binding protein. Hemagglutin ...
DOI:
10.1016/j.ijbiomac.2017.05.037

23. Peptidase compensation in the digestive system of whiteleg shrimp Penaeus vannamei against dietary Kunitz‐type soybean trypsin inhibitor

Author:
Maytorena‐Verdugo, C.I.; Córdova‐Murueta, J.H.; García‐Carreño, F.L.
Source:
Aquaculture nutrition 2017 v.23 no.5 pp. 1095-1103
ISSN:
1353-5773
Subject:
trypsin inhibitors,   etc   ; Litopenaeus vannamei; bioassays; chymotrypsin; enzyme activity; feces; proteolysis; serine; shrimp; soybean meal; soybeans; trypsin;     Show all 12 Subjects
Abstract:
... The aim of this study was to assess the regulatory process of digestive peptidases of crustaceans in the presence of soybean trypsin inhibitor (SBTI). This naturally occurring inhibitor in soybean meal was used to inhibit the activity of digestive serine peptidases of the whiteleg shrimp Litopenaeus vannamei. In vitro, SBTI inhibited the total proteolytic activity and chymotrypsin activity by 65%. ...
DOI:
10.1111/anu.12477

24. Application and bioactive properties of CaTI, a trypsin inhibitor from Capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells

Author:
Silva, Marciele S; Ribeiro, Suzanna FF; Taveira, Gabriel B; Rodrigues, Rosana; Fernandes, Katia VS; Carvalho, André O; Vasconcelos, Ilka Maria; Mello, Erica Oliveira; Gomes, Valdirene M
Source:
Journal of the science of food and agriculture 2017 v.97 no.11 pp. 3790-3801
ISSN:
0022-5142
Subject:
trypsin inhibitors,   etc   ; Capsicum annuum; Fusarium oxysporum; Glomerella cingulata; antifungal properties; antimicrobial peptides; enzyme activity; fluorescein; hot peppers; hyphae; isothiocyanates; mechanism of action; nitric oxide; oxidative stress; plant pathogenic fungi; reactive oxygen species; seeds; trypsin;     Show all 18 Subjects
Abstract:
... BACKGROUND: During the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. Recent results from our laboratory have shown the purification of a new trypsin inhibitor, named CaTI, from chilli pepper (Capsicum annuum L.) seeds. This study aims to evaluate the antifungal activity and mechanism of action of CaTI on phytopathogenic fungi ...
DOI:
10.1002/jsfa.8243

25. Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study

Author:
Cheng, Mengxia; Chen, Zilin
Source:
Journal of separation science 2017 v.40 no.15 pp. 3168-3174
ISSN:
1615-9306
Subject:
trypsin inhibitors,   etc   ; Oriental traditional medicine; baicalin; capillary electrophoresis; enzyme kinetics; immobilized enzymes; molecular models; screening; trypsin;     Show all 9 Subjects
Abstract:
... A trypsin immobilized enzyme microreactor was successfully prepared in capillary for studying enzyme kinetics of trypsin and online screening of trypsin inhibitors from traditional Chinese medicine through capillary electrophoresis. Trypsin was immobilized on the inner wall at the inlet of the capillary treated with polydopamine. The rest of the capillary was used as a separation channel. The para ...
DOI:
10.1002/jssc.201700419

26. A case of neofunctionalization of a Putranjiva roxburghii PNP protein to trypsin inhibitor by disruption of PNP-UDP domain through an insert containing inhibitory site

Author:
Verma, Preeti; Patel, Girijesh K.; Kar, Bibekananda; Sharma, Ashwani K.
Source:
Plant science 2017 v.260 pp. 19-30
ISSN:
0168-9452
Subject:
trypsin inhibitors,   etc   ; Putranjiva roxburghii; active sites; divergent evolution; enzyme activity; guanosine; plant proteins; sequence analysis; storage proteins; trypsin;     Show all 10 Subjects
Abstract:
... The attainment of new function by a protein is achieved through convergent/divergent evolution. In present work, the sequence analysis of a 34kDa protein from Putranjiva roxburghii, earlier reported as a potent trypsin inhibitor, showed resemblance to some of the wound inducible and vegetative storage proteins. A detailed sequence analysis revealed that these proteins belong to PNP-UDP family. In ...
DOI:
10.1016/j.plantsci.2017.03.013

27. A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activity against Aedes aegypti

Author:
Dias, Lucas P.; Oliveira, Jose T.A.; Rocha-Bezerra, Lady C.B.; Sousa, Daniele O.B.; Costa, Helen P.S.; Araujo, Nadine M.S.; Carvalho, Ana F.U.; Tabosa, Pedro M.S.; Monteiro-Moreira, Ana C.O.; Lobo, Marina D.P.; Moreno, Frederico B.M.B.; Rocha, Bruno A.M.; Lopes, José L.S.; Beltramini, Leila M.; Vasconcelos, Ilka M.
Source:
Process biochemistry 2017 v.57 pp. 228-238
ISSN:
1359-5113
Subject:
trypsin inhibitors,   etc   ; Aedes aegypti; Cassia; acute toxicity; amino acid sequences; cattle; chromatography; dithiothreitol; glycoproteins; inhibitory concentration 50; insecticidal properties; instars; larvae; lethal concentration 50; mass spectrometry; midgut; mortality; pH; polypeptides; seeds; temperature; trypsin;     Show all 22 Subjects
Abstract:
... A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI was purified by DEAE-Cellulose and trypsin-Sepharose 4B chromatography, with a 15.5-fold purification and 2.4% yield. ClTI is composed of a 19,484Da polypeptide chain as revealed by mass spectrometry, it is not a glycoprotein, its amino acid s ...
DOI:
10.1016/j.procbio.2017.03.015

28. Purification and antiparasitic activity of a few legume serine proteinase inhibitors: Effect on erythrocyte invasion, schizont rupture and proteolytic processing of the Plasmodium falciparum AMA1 protein

Author:
Bhattacharyya, Arindam; Chitnis, Chetan E.; Babu, C.R.
Source:
Process biochemistry 2017 v.57 pp. 207-218
ISSN:
1359-5113
Subject:
trypsin inhibitors,   etc   ; Archidendron; Derris trifoliata; Plasmodium falciparum; additive effect; adverse effects; antigens; antimalarials; antiparasitic properties; chymotrypsin; erythrocytes; high performance liquid chromatography; inhibitory concentration 50; merozoites; parasites; proteins; proteolysis; soybeans; synergism; trypsin;     Show all 20 Subjects
Abstract:
... Legume proteinase inhibitors (PI/s) abrogate proteolytic activity of proteins and cause adverse effects on growth. In this study, two legume PIs – Archidendron ellipticum Trypsin Inhibitor (AeTI) and Derris trifoliata Trypsin Chymotrypsin Inhibitor (DtTCI) were purified and used along with standard, Soybean Trypsin Inhibitor (STI), either singly or in combination, as antiplasmodial agents against ...
DOI:
10.1016/j.procbio.2017.03.011

29. Litopenaeus vannamei digestive metallo peptidases compensate for anti-nutritional SBTI in feed

Author:
Maytorena-Verdugo, Claudia I.; Córdova-Murueta, Julio H.; García-Carreño, Fernando L.
Source:
Aquaculture 2017 v.473 pp. 508-512
ISSN:
0044-8486
Subject:
trypsin inhibitors,   etc   ; Litopenaeus vannamei; chymotrypsin; homeostasis; ingestion; phenotype; proteolysis; shrimp; soybeans; trypsin;     Show all 10 Subjects
Abstract:
... The aim of this work was to study mechanisms of homeostasis of the digestive system of the whiteleg shrimp Litopenaeus vannamei when fed anti-nutritional soybean trypsin inhibitor (SBTI). In a previous work, we tested if the shrimp could respond against SBTI in feed with the increase in the digestive proteolytic activity. This time we tested the time of response against the inhibitor and also how ...
DOI:
10.1016/j.aquaculture.2017.02.023

30. Aggregation of trypsin and trypsin inhibitor by Al cation

Author:
Chanphai, P.; Kreplak, L.; Tajmir-Riahi, H.A.
Source:
Journal of photochemistry and photobiology 2017 v.169 pp. 7-12
ISSN:
1011-1344
Subject:
trypsin inhibitors,   etc   ; Fourier transform infrared spectroscopy; aluminum; atomic force microscopy; cations; hydrogen bonding; neurodegenerative diseases; proteins; t-test; thermodynamics; trypsin; van der Waals forces;     Show all 12 Subjects
Abstract:
... Al cation may trigger protein structural changes such as aggregation and fibrillation, causing neurodegenerative diseases. We report the effect of Al cation on the solution structures of trypsin (try) and trypsin inhibitor (tryi), using thermodynamic analysis, UV–Visible, Fourier transform infrared (FTIR) spectroscopic methods and atomic force microscopy (AFM). Thermodynamic parameters showed Al-p ...
DOI:
10.1016/j.jphotobiol.2017.02.018

31. Major trypsin like‐serine proteinases from albacore tuna (Thunnus alalunga) spleen: Biochemical characterization and the effect of extraction media

Author:
Poonsin, Tanchanok; Sripokar, Pakteera; Benjakul, Soottawat; Simpson, Benjamin K.; Visessanguan, Wonnop; Klomklao, Sappasith
Source:
Journal of food biochemistry 2017 v.41 no.2
ISSN:
0145-8884
Subject:
trypsin inhibitors,   etc   ; Thunnus alalunga; buffers; detergents; electrophoresis; fluorides; leather; molecular weight; pH; pollution; processing waste; proteolysis; silk; sodium chloride; sodium phosphate; soybeans; spleen; temperature; trypsin; tuna;     Show all 20 Subjects
Abstract:
... This investigation aimed to characterize the proteinases and to study the effect of extraction media on proteinases recovery from albacore tuna spleen. Optimal activity of splenic extract was at pH 9.5 and 55°C. The enzyme was stable in a wide pH range of 6.0–10.0 but unstable at the temperatures greater than 50°C for 30–120 min. The proteolytic activity was strongly inhibited by soybean trypsin i ...
DOI:
10.1111/jfbc.12323

32. Peanut Seed Cultivars with Contrasting Resistance to Aspergillus parasiticus Colonization Display Differential Temporal Response of Protease Inhibitors

Author:
Müller, Virginia; Bonacci, Gustavo; Batthyany, Carlos; Amé, María V.; Carrari, Fernando; Gieco, Jorge; Asis, Ramón
Source:
Phytopathology 2017 v.107 no.4 pp. 474-482
ISSN:
0031-949X
Subject:
trypsin inhibitors,   etc   ; Aspergillus parasiticus; aflatoxins; antifungal properties; chromatography; cultivars; fungi; genes; peanuts; seeds; spectroscopy; trypsin;     Show all 12 Subjects
Abstract:
... Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant protease inhibitors (PPIs) produced in peanut seeds of the resistant PI 337394 and the susceptible Forman ...
DOI:
10.1094/PHYTO-09-16-0346-R

33. Physicochemical characterization of jicaro seeds (Crescentia alata H.B.K.): A novel protein and oleaginous seed

Author:
Corrales, C.V.; Fliedel, G.; Perez, A.M.; Servent, A.; Prades, A.; Dornier, M.; Lomonte, B.; Vaillant, F.
Source:
Subtropical plant science 2017 v.56 pp. 84-92
ISSN:
0889-1575
Subject:
trypsin inhibitors,   etc   ; polyphenols; polyacrylamide gel electrophoresis; drought; trees; digestibility; oleic acid; trypsin; sugars; molecular weight; dietary fiber; phytic acid; albumins; proteomics; Crescentia alata; fruits; soybeans; essential amino acids; cotyledons; Central America;     Show all 20 Subjects
Abstract:
... Jicaro (Crescentia alata) is a tropical tree native to Mesoamerica well adapted to severe drought conditions. The seeds of the fruits were analyzed for protein, fatty acid, dietary fiber, phytate, polyphenol, tannin, tocopherol, sugar, mineral, amino acid and trypsin inhibitor contents. The jicaro cotyledons contained 43.6±1.15g protein/100g and 38.0±0.20g fat/100g (d.w.), which is comparable to m ...
DOI:
10.1016/j.jfca.2016.11.007

34. Trypsin and trypsin inhibitor bind milk beta-lactoglobulin: Protein–protein interactions and morphology

Author:
Chanphai, P.; Tajmir-Riahi, H.A.
Source:
International journal of biological macromolecules 2017 v.96 pp. 754-758
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; aqueous solutions; beta-lactoglobulin; binding capacity; hydrogen bonding; milk; pH; protein-protein interactions; spectroscopy; thermodynamics; transmission electron microscopy; trypsin; van der Waals forces;     Show all 13 Subjects
Abstract:
... Conjugation of trypsin and trypsin inhibitor with milk beta-lactoglobulin (b-LG) was studied in aqueous solution at physiological pH. Multiple spectroscopic methods and transmission electron microscopy (TEM) were used to characterize protein–protein interactions and protein morphology. Thermodynamic analysis ΔH (−24 to −11kJMol−1), ΔS (−30 to −5JMol−1K−1) and ΔG (−12 to −10kJMol−1) showed that pro ...
DOI:
10.1016/j.ijbiomac.2016.12.075

35. Characterization of Endogenous Protease and the Changes in Proteolytic Activity of Acetes vulgaris and Macrobrachium lanchesteri During Kapi Production

Author:
Pongsetkul, Jaksuma; Benjakul, Soottawat; Sumpavapol, Punnanee; Osako, Kazufumi; Faithong, Nandhsha
Source:
Journal of food biochemistry 2017 v.41 no.1
ISSN:
0145-8884
Subject:
trypsin inhibitors,   etc   ; Acetes; Macrobrachium; actin; bacteria; chymotrypsin; condiments; fermentation; fluorides; hydrolysis; myosin heavy chains; oligopeptides; pH; palatability; proteolysis; raw materials; salting; shrimp; sodium chloride; soybeans; trypsin;     Show all 21 Subjects
Abstract:
... Characteristics of endogenous proteases of shrimp, Acetes vulgaris (AP) and Macrobrachium lanchesteri (MP) as well as the changes in proteolytic activity during Kapi production were investigated. Maximal activity of AP and MP was found at pH 7, 60C and pH 8, 60C, respectively. Activity of both proteases decreased with increasing NaCl concentration (0–30%). Both extracts were strongly inhibited by ...
DOI:
10.1111/jfbc.12311

36. Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses

Author:
Acevedo, Flor E.; Peiffer, Michelle; Tan, Ching-Wen; Stanley, Bruce A.; Stanley, Anne; Wang, Jie; Jones, Asher G.; Hoover, Kelli; Rosa, Cristina; Luthe, Dawn; Felton, Gary
Source:
Molecular plant-microbe interactions 2017 v.30 no.2 pp. 127-137
ISSN:
0894-0282
Subject:
trypsin inhibitors,   etc   ; Klebsiella; Pantoea ananatis; Raoultella; Spodoptera frugiperda; bacteria; catechol oxidase; corn; gene expression regulation; genes; herbivores; host plants; insect larvae; insects; intestinal microorganisms; mechanical damage; peroxidase; proteins; saliva; tomatoes; trypsin;     Show all 21 Subjects
Abstract:
... Mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid–mediated defense responses in plants. This study investigated the effects of bacteria from oral secretions of the fall armyworm Spodoptera frugiperda on herbivore-induced defenses in tomato and maize plants. Using culture-dependent methods, we identified s ...
DOI:
10.1094/MPMI-11-16-0240-R

37. Psc-AFP from Psoralea corylifolia L. overexpressed in Pichia pastoris increases antimicrobial activity and enhances disease resistance of transgenic tobacco

Author:
Luo, Xiu-Mei; Xie, Cheng-Jian; Wang, De; Wei, Yun-Min; Cai, Jie; Cheng, Shan-Shan; Yang, Xing -Yong; Sui, An -Ping
Source:
Applied microbiology and biotechnology 2017 v.101 no.3 pp. 1073-1084
ISSN:
0175-7598
Subject:
trypsin inhibitors,   etc   ; Alternaria alternata; Alternaria brassicae; Aspergillus niger; Cullen corylifolium; Pichia pastoris; Ralstonia solanacearum; Thanatephorus cucumeris; amino acids; antimicrobial properties; antimicrobial proteins; bioassays; complementary DNA; conidia; disease resistance; gene overexpression; genes; germination; molecular cloning; mycelium; plant growth; rapid amplification of cDNA ends; seeds; signal peptide; tobacco; transgenic plants; trypsin; yeasts;     Show all 28 Subjects
Abstract:
... Psc-AFP, isolated from the seeds of Psoralea corylifolia L., is an antimicrobial protein with trypsin inhibitor activity. Its encoding gene was cloned by 3′- rapid amplification of cDNA ends (RACE) combined with Y-shaped adaptor-dependent extension (YADE) method. The gene Psc-AFP encodes a protein of 203 amino acids with a deduced signal peptide of 24 residues. The growth inhibition effect exerted ...
DOI:
10.1007/s00253-016-7768-1

38. Antioxidative Activitiy of Protein Hydrolysate from the Muscle of Common Kilka (Clupeonella cultriventris caspia) Prepared Using the Purified Trypsin from Common Kilka Intestine

Author:
Zamani, Abbas; Madani, Rasool; Rezaei, Masoud; Benjakul, Soottawat
Source:
Journal of aquatic food product technology 2017 v.26 no.1 pp. 2-16
ISSN:
1547-0636
Subject:
trypsin inhibitors,   etc   ; 2,2-diphenyl-1-picrylhydrazyl; Clupeonella cultriventris; ammonium sulfate; antioxidant activity; chromatography; fish protein hydrolysate; hydrolysates; hydrolysis; intestines; molecular weight; muscles; pH; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; soybeans; trypsin;     Show all 17 Subjects
Abstract:
... Trypsin from the intestine of common kilka (Clupeonella cultriventris caspia) was purified using ammonium sulfate precipitation (30–50% saturation), Sephadex G-75, and DEAE-cellulose chromatography with the purity of 30-fold and the yield of 12%. The molecular weight of trypsin was estimated to be 23.2 kDa based on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The trypsin ...
DOI:
10.1080/10498850.2015.1073822

39. Continuous method to determine the trypsin inhibitor activity in soybean flour

Author:
Coscueta, Ezequiel R.; Pintado, Manuela E.; Picó, Guillermo A.; Knobel, Gastón; Boschetti, Carlos E.; Malpiedi, Luciana Pellegrini; Nerli, Bibiana B.
Source:
Food chemistry 2017 v.214 pp. 156-161
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; acetic acid; analytical methods; centrifugation; filtration; nutritive value; soy flour; trypsin; turbidity;     Show all 9 Subjects
Abstract:
... The determination of trypsin inhibitor (TI) activity is of importance to evaluate the nutritional value of soybean flours. An analytical method, which involves a continuous spectrophotometric rate determination for trypsin activity against the substrate N-benzoyl-DL-arginine p-nitroanilide, is proposed as an alternative to the standard discontinuous assay. Stopping the reaction with acetic acid an ...
DOI:
10.1016/j.foodchem.2016.07.056

40. Elucidation of different cold-adapted Atlantic cod (Gadus morhua) trypsin X isoenzymes Proteins and proteomics

Author:
Stefansson, Bjarki; Sandholt, Gunnar B.; Gudmundsdottir, Ágústa
Source:
Biochimica et biophysica acta 2017 v.1865 no.1 pp. 11-19
ISSN:
1570-9639
Subject:
trypsin inhibitors,   etc   ; Gadus morhua; amino acids; anion exchange chromatography; catalytic activity; complementary DNA; isozymes; mass spectrometry; pH; proteins; trypsin;     Show all 11 Subjects
Abstract:
... Trypsins from Atlantic cod (Gadus morhua), consisting of several isoenzymes, are highly active cold-adapted serine proteases. These trypsins are isolated for biomedical use in an eco-friendly manner from underutilized seafood by-products. Our group has explored the biochemical properties of trypsins and their high potential in biomedicine. For broader utilization of cod trypsins, further character ...
DOI:
10.1016/j.bbapap.2016.10.005

41. Natural structural diversity within a conserved cyclic peptide scaffold

Author:
Elliott, Alysha G.; Franke, Bastian; Armstrong, David A.; Craik, David J.; Mylne, Joshua S.; Rosengren, K. Johan
Source:
Amino acids 2017 v.49 no.1 pp. 103-116
ISSN:
0939-4451
Subject:
trypsin inhibitors,   etc   ; Helianthus annuus; amino acids; disulfide bonds; drugs; nuclear magnetic resonance spectroscopy; peptides; pharmaceutical industry; proteins; seeds; trypsin;     Show all 11 Subjects
Abstract:
... We recently isolated and described the evolutionary origin of a diverse class of small single-disulfide bonded peptides derived from Preproalbumin with SFTI-1 (PawS1) proteins in the seeds of flowering plants (Asteraceae). The founding member of the PawS derived peptide (PDP) family is the potent trypsin inhibitor SFTI-1 (sunflower trypsin inhibitor-1) from Helianthus annuus, the common sunflower. ...
DOI:
10.1007/s00726-016-2333-x

42. Trypsin inhibitor from Leucaena leucocephala seeds delays and disrupts the development of Aedes aegypti, a multiple‐disease vector

Author:
Almeida Filho, Luiz CP; de Souza, Terezinha M; Tabosa, Pedro MS; Soares, Nayana G; Rocha‐Bezerra, Lady CB; Vasconcelos, Ilka M; Carvalho, Ana FU
Source:
Pest management science 2017 v.73 no.1 pp. 181-187
ISSN:
1526-498X
Subject:
trypsin inhibitors,   etc   ; Aedes aegypti; Leucaena leucocephala; Yellow fever virus; acute toxicity; agarose; chromatography; death; dengue; digestion; disease vectors; eggs; enzyme activity; hatching; insects; larvae; larval development; midgut; molecular weight; mortality; pest management; polyacrylamide gel electrophoresis; proteins; proteolysis; public health; seeds; sodium dodecyl sulfate; trichloroacetic acid precipitation; trypsin;     Show all 29 Subjects
Abstract:
... BACKGROUND: Nowadays, the Aedes aegypti mosquito represents a serious public health issue in view of the large outbreaks of the arboviral diseases zika, dengue, chikungunya and yellow fever. This holometabolous insect has midgut digestive enzymes that are trypsin‐ and chymotrypsin‐like proteins. Protease inhibitors are able to bind to proteolytic enzymes and promote a blockage in digestion and nut ...
DOI:
10.1002/ps.4284

43. An ultrasensitive fluorescent nanosensor for trypsin based on upconversion nanoparticles

Author:
Wu, Mingmin; Wang, Xiaoyong; Wang, Kun; Guo, Zijian
Source:
Talanta 2017
ISSN:
0039-9140
Subject:
trypsin inhibitors,   etc   ; energy transfer; fluorescence; nanogold; nanoparticles; toxicity; trypsin;     Show all 7 Subjects
Abstract:
... Trypsin and its inhibitors are relevant to many physiological processes and diseases. In this study, a nanosensor capable of detecting trypsin and its inhibitors was designed based on the fluorescence resonance energy transfer (FRET) between upconversion nanoparticle (UCNP) and gold nanoparticle (AuNP). UCNP and AuNP were linked by a trypsin-sensitive peptide DDDDARC, forming the non-fluorescent U ...
DOI:
10.1016/j.talanta.2017.07.013

44. Identification of peptides in the terminal ileum of broiler chickens fed diets based on maize and soybean meal using proteomics

Author:
Cowieson, A. J.; Klausen, M.; Pontoppidan, K.; Faruk, M. Umar; Roos, F. F.; Giessing, A. M. B.
Source:
Animal production science 2017 v.57 no.8 pp. 1738-1750
ISSN:
1836-0939
Subject:
trypsin inhibitors,   etc   ; agglutinins; albumins; alkaline phosphatase; amino acid sequences; aminopeptidases; animal models; broiler chickens; cages; corn; databases; digesta; digestibility; digestion; energy; feed conversion; feed intake; freeze drying; globulins; glycinin; ileum; nitrogen; peptides; prolamins; protein secretion; proteomics; soy protein; soybean meal; soybeans; trypsin; weight gain;     Show all 31 Subjects
Abstract:
... A total of 160 Ross PM3 birds were used in a two treatment feeding study in order to explore the usefulness of proteomics to identify the origin of peptides in ileal digesta. Two diets were fed, one conventional maize/soy-based diet acted as a reference whereas a second diet, formulated to be nutritionally equivalent to the reference diet in protein and energy provision, contained 20% raw soy meal ...
DOI:
10.1071/AN16213

45. Identification and characterisation of serine protease inhibitors from Araucaria angustifolia seeds

Author:
Alves, Flávio Lopes; Sallai, Roberto C.; Salu, Bruno R.; Miranda, Antonio; Oliva, Maria Luiza V.
Source:
Natural product research 2016 v.30 no.23 pp. 2712-2715
ISSN:
1478-6427
Subject:
trypsin inhibitors,   etc   ; Araucaria angustifolia; acetone; affinity chromatography; alpha-amylase; chymotrypsin; coagulation; high performance liquid chromatography; humans; molecular weight; plasma kallikrein; plasmin; polyacrylamide gel electrophoresis; proteins; seeds; spectrometers; starch; swine; trypsin;     Show all 19 Subjects
Abstract:
... Araucaria angustifolia seeds are characterised by a relatively high content of starch and protein. This study aimed to verify the presence of α-amylase inhibitors in the seeds and to characterise a trypsin inhibitor found in the embryo tissues. Inhibitor purification was carried out by the saline extraction of proteins, acetone precipitation and affinity chromatography. Two protein bands of molecu ...
DOI:
10.1080/14786419.2015.1136887

46. Label–free, turn–on fluorescent sensor for trypsin activity assay and inhibitor screening

Author:
Zhang, Lufeng; Qin, Haiyan; Cui, Wanwan; Zhou, Yang; Du, Jianxiu
Source:
Talanta 2016 v.161 pp. 535-540
ISSN:
0039-9140
Subject:
trypsin inhibitors,   etc   ; cytochrome c; detection limit; diagnostic techniques; drugs; fluorescence; inhibitory concentration 50; oxidation; pancreas; screening; soybeans; statistical analysis; thiamin; trypsin;     Show all 14 Subjects
Abstract:
... The development of new detection methods for proteases activity assay is important in clinical diagnostics and drug development. In this work, a simple, label–free, and turn–on fluorescent sensor was fabricated for trypsin, a protease produced in the pancreas. Cytochrome c, a natural substance of trypsin, could be selectively cleaved by trypsin into heme–peptide fragment. The produced heme–peptide ...
DOI:
10.1016/j.talanta.2016.09.011

47. Bovine pancreatic trypsin inhibitor immobilized onto sepharose as a new strategy to purify a thermostable alkaline peptidase from cobia (Rachycentron canadum) processing waste B Analytical technologies in the biomedical and life sciences

Author:
França, Renata Cristina da Penha; Assis, Caio Rodrigo Dias; Santos, Juliana Ferreira; Torquato, Ricardo José Soares; Tanaka, Aparecida Sadae; Hirata, Izaura Yoshico; Assis, Diego Magno; Juliano, Maria Aparecida; Cavalli, Ronaldo Olivera; Carvalho Jr, Luiz Bezerra de; Bezerra, Ranilson Souza
Source:
Journal of chromatography 2016 v.1033-1034 pp. 210-217
ISSN:
1570-0232
Subject:
trypsin inhibitors,   etc   ; Rachycentron canadum; activation energy; agarose; amino acid sequences; cattle; chromatography; cold; fish; hydrolysis; mass spectrometry; molecular weight; pH; polyacrylamide gel electrophoresis; processing waste; sodium dodecyl sulfate; temperature; thermal stability; trypsin;     Show all 19 Subjects
Abstract:
... A thermostable alkaline peptidase was purified from the processing waste of cobia (Rachycentron canadum) using bovine pancreatic trypsin inhibitor (BPTI) immobilized onto Sepharose. The purified enzyme had an apparent molecular mass of 24kDa by both sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry. Its optimal temperature and pH were 50°C and 8.5, respecti ...
DOI:
10.1016/j.jchromb.2016.08.028

48. Autolysis and Characterization of Sarcoplasmic and Myofibril Associated Proteinases of Oxeye Scad (Selar boops) Muscle

Author:
Wongwichian, Chantira; Chaijan, Manat; Panpipat, Worawan; Klomklao, Sappasith; Benjakul, Soottawat
Source:
Journal of aquatic food product technology 2016 v.25 no.7 pp. 1132-1143
ISSN:
1547-0636
Subject:
trypsin inhibitors,   etc   ; Selar; autolysis; beta-mercaptoethanol; calcium chloride; dithiothreitol; fluorides; gel strength; guidelines; muscles; pH; proteolysis; sodium chloride; soybeans; surimi; temperature; trypsin;     Show all 17 Subjects
Abstract:
... The autolytic profile of oxeye scad mince was characterized. Mince showed higher proteolytic activity than washed mince. The highest autolysis was observed at 65 and 60°C for mince and washed mince, respectively. Both mince and washed mince showed the optimum pH for autolysis at pH 9.0, and their activities decreased with increasing NaCl concentration (0–3.5%). Autolysis of washed mince was marked ...
DOI:
10.1080/10498850.2015.1032462

49. Purification and characterization of a novel heterodimer protease inhibitor from Streptomyces spp. VL J2 with potential biopesticidal activity against H. armigera

Author:
Marathe, Kiran; Kasar, Sainath; Chaudhari, Ambalal; Maheshwari, Vijay
Source:
Process biochemistry 2016 v.51 no.10 pp. 1650-1663
ISSN:
1359-5113
Subject:
trypsin inhibitors,   etc   ; Streptomyces; active sites; arginine; chymotrypsin; isoelectric point; larvae; molecular weight; nutrition assessment; pH; pesticidal properties; polyacrylamide gel electrophoresis; pupae; staining; stoichiometry; transgenes; transgenic plants; trypsin;     Show all 18 Subjects
Abstract:
... A protease inhibitor (PI) was isolated and purified from halo-alkaliphilic Streptomyces spp. VL J2. SDS-PAGE of purified PI revealed it to be a heterodimer of two unidentical subunits of 27.5 and 11.08kDa, which corroborates well with intact molecular mass of 38.5kDa obtained by GPC and MALDI −TOF. Inhibitory activity was confirmed by activity staining and reverse zymogram studies and the inhibito ...
DOI:
10.1016/j.procbio.2016.08.010

50. BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells

Author:
Dia, Vermont P.; Krishnan, Hari B.
Source:
Scientific Reports 2016 v.6 pp. 1-12
ISSN:
2045-2322
Subject:
trypsin inhibitors,   etc   ; Momordica charantia; anticarcinogenic activity; apoptosis; carcinogenesis; caspase-3; cell cycle; colon; colorectal neoplasms; cyclin-dependent kinase; cytotoxicity; enzyme inhibition; humans; neoplasm cells; peptides; perennials; proteins; soybeans; trypsin;     Show all 19 Subjects
Abstract:
... Momordica charantia is a perennial plant with reported health benefits. BG-4, a novel peptide from Momordica charantia, was isolated, purified and characterized. The trypsin inhibitory activity of BG-4 is 8.6 times higher than purified soybean trypsin inhibitor. The high trypsin inhibitory activity of BG-4 may be responsible for its capability to cause cytotoxicity to HCT-116 and HT-29 human colon ...
Handle:
10113/63140
DOI:
10.1038/srep33532
PubMed:
27628414
PubMed Central:
PMC5024301