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- Buckley, Jim, et al. Show all 5 Authors
- Journal of microbiological methods 2012 v.88 no.1 pp. 172-174
- Mycobacterium avium subsp. paratuberculosis; cattle; genotyping; melting; polymerase chain reaction; sheep
- ... In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity. ...
- Buckley, Jim, et al. Show all 7 Authors
- Applied and environmental microbiology 2009 v.75 no.24 pp. 7870-7872
- Mycobacterium avium subsp. paratuberculosis; paratuberculosis; rifampicin; screening; viability
- ... A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10⁴ to 10⁸ CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis. ...