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- Olson, DanielG., et al. Show all 8 Authors
- Biotechnology for biofuels 2017 v.10 no.1 pp. 276
- Clostridium thermocellum; NAD (coenzyme); Thermoanaerobacterium saccharolyticum; biofuels; biomass; ethanol; genes; glyceraldehyde-3-phosphate dehydrogenase; industrial applications; metabolic engineering; metabolites; metabolomics; mutation
- ... BACKGROUND: Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer produced by strains developed thus far is an obstacle to industrial application. RESULTS: Here, we analyzed changes in the relative concentration of intracellular metabolites in response to gradual addition of ethanol to growing cu ...
- Olson, DanielG., et al. Show all 10 Authors
- Biotechnology for biofuels 2017 v.10 no.1 pp. 282
- Thermoanaerobacterium saccharolyticum; acetates; alcohol dehydrogenase; anaerobes; cellulose; enzyme activity; ethanol; ethanol production; genes; glucose; hemicellulose; hydrogen; thermophilic bacteria
- ... BACKGROUND: With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compoun ...