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1. A Facile N-Mercaptoethoxyglycinamide (MEGA) Linker Approach to Peptide Thioesterification and Cyclization

Author:
Shelton, Patrick M. M.; Weller, Caroline E.; Chatterjee, Champak
Source:
Journal of the American Chemical Society 2017 v.139 no.11 pp. 3946-3949
ISSN:
1520-5126
Subject:
trypsin inhibitors,   etc   ; amino acids; cyclic peptides; pH;     Show all 4 Subjects
Abstract:
... The C-terminal electrophilic activation of peptides by α-thioesterification requires strongly acidic conditions or complex chemical manipulations, which ultimately limit functional group compatibility and broad utility. Herein, we report a readily accessible N-mercaptoethoxyglycinamide (MEGA) solid-phase linker for the facile synthesis of latent peptide α-thioesters. Incubating peptide-MEGA sequen ...
DOI:
10.1021/jacs.6b13271
PubMed:
28230996
PubMed Central:
PMC5731643

2. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis

Author:
Baia-da-Silva, Djane Clarys; Alvarez, Luis Carlos Salazar; Lizcano, Omaira Vera; Costa, Fabio Trindade Maranhão; Lopes, Stefanie Costa Pinto; Orfanó, Alessandra Silva; Pascoal, Denner Oliveira; Nacif-Pimenta, Rafael; Rodriguez, Iria Cabral; Guerra, Maria das Graças Vale Barbosa; Lacerda, Marcus Vinicius Guimarães; Secundino, Nagila Francinete Costa; Monteiro, Wuelton Marcelo; Pimenta, Paulo Filemon Paolucci
Source:
Parasites & vectors 2018 v.11 no.1 pp. 148
ISSN:
1756-3305
Subject:
trypsin inhibitors,   etc   ; Anopheles aquasalis; Plasmodium vivax; blood meal; chitinase; digestion; digestive enzymes; enzyme activity; erythrocytes; ingestion; insect vectors; malaria; parasites; pathogens; peritrophic membrane; proteolysis; Amazonia;     Show all 17 Subjects
Abstract:
... BACKGROUND: Plasmodium vivax is predominant in the Amazon region, and enhanced knowledge of its development inside a natural vector, Anopheles aquasalis, is critical for future strategies aimed at blocking parasite development. The peritrophic matrix (PM), a chitinous layer produced by the mosquito midgut in response to blood ingestion, is a protective barrier against pathogens. Plasmodium can onl ...
DOI:
10.1186/s13071-018-2752-5
PubMed:
29510729
PubMed Central:
PMC5840820

3. Heterochiral Knottin Protein: Folding and Solution Structure

Author:
Mong, Surin K.; Cochran, Frank V.; Yu, Hongtao; Graziano, Zachary; Lin, Yu-Shan; Cochran, Jennifer R.; Pentelute, Bradley L.
Source:
Biochemistry 2017 v.56 no.43 pp. 5720-5725
ISSN:
1520-4995
Subject:
trypsin inhibitors,   etc   ; Ecballium elaterium; Gibbs free energy; beta-alanine; molecular dynamics; nuclear magnetic resonance spectroscopy; optical isomerism; proteolysis; scaffolding proteins;     Show all 9 Subjects
Abstract:
... Homochirality is a general feature of biological macromolecules, and Nature includes few examples of heterochiral proteins. Herein, we report on the design, chemical synthesis, and structural characterization of heterochiral proteins possessing loops of amino acids of chirality opposite to that of the rest of a protein scaffold. Using the protein Ecballium elaterium trypsin inhibitor II, we discov ...
DOI:
10.1021/acs.biochem.7b00722
PubMed:
28952732
PubMed Central:
PMC5818992

4. Spatial Heat Maps from Fast Information Matching of Fast and Slow Degrees of Freedom: Application to Molecular Dynamics Simulations

Author:
Kovacs, Julio A.; Wriggers, Willy
Source:
The Journal of physical chemistry 2016 v.120 no.33 pp. 8473-8484
ISSN:
1520-5207
Subject:
trypsin inhibitors,   etc   ; cattle; chemical structure; computer simulation; heat; molecular dynamics; prediction; time series analysis;     Show all 8 Subjects
Abstract:
... We introduce a fast information matching (FIM) method for transforming time domain data into spatial images through handshaking between fast and slow degrees of freedom. The analytics takes advantage of the detailed time series available from biomolecular computer simulations, and it yields spatial heat maps that can be visualized on 3D molecular structures or in the form of interaction networks. ...
DOI:
10.1021/acs.jpcb.6b02136
PubMed:
27169521
PubMed Central:
PMC5545105

5. Evaluation of procoagulant tissue factor expression in canine hemangiosarcoma cell lines

Author:
Witter, Lauren E.; Gruber, Eirka J.; Lean, Fabian Z. H.; Stokol, Tracy
Source:
American journal of veterinary research 2017 v.78 no.1 pp. 69-79
ISSN:
0002-9645
Subject:
trypsin inhibitors,   etc   ; antigens; biomarkers; brain; coagulation; corn; dogs; endothelial cells; flow cytometry; frogs; hemangiosarcoma; humans; messenger RNA; metastasis; phosphatidylserines; quantitative polymerase chain reaction; thrombin;     Show all 17 Subjects
Abstract:
... OBJECTIVE To evaluate expression of procoagulant tissue factor (TF) by canine hemangiosarcoma cells in vitro. SAMPLES 4 canine hemangiosarcoma cell lines (SB-HSA [mouse-passaged cutaneous tumor], Emma [primary metastatic brain tumor], and Frog and Dal-1 [primary splenic tumors]) and 1 nonneoplastic canine endothelial cell line (CnAoEC). PROCEDURES TF mRNA and TF antigen expression were evaluated b ...
DOI:
10.2460/ajvr.78.1.69
PubMed:
28029283
PubMed Central:
PMC5299388

6. Herbivore associated elicitor-induced defences are highly specific among closely related Nicotiana species

Author:
Xu, Shuqing; Zhou, Wenwu; Pottinger, Sarah; Baldwin, Ian T
Source:
BMC plant biology 2015 v.15 no.1 pp. 2
ISSN:
1471-2229
Subject:
trypsin inhibitors,   etc   ; Manduca sexta; Nicotiana; Spodoptera littoralis; divergent evolution; elicitors; herbivores; induced resistance; insects; oviposition;     Show all 10 Subjects
Abstract:
... BACKGROUND: Herbivore-induced defence responses are often specific - different herbivores induce different defence responses in plants - and their specificity is largely mediated by chemical cues (herbivore-associated elicitors: HAEs) in insect oral or oviposition secretions. However, the specificity and the mechanisms of HAE-induced defence have not been investigated in the context of the evoluti ...
DOI:
10.1186/s12870-014-0406-0
PubMed:
25592329
PubMed Central:
PMC4304619

7. Optimization of low-abundance protein extraction and abundant protein removal from defatted soybean meal

Author:
Liu, Ming-mei; Qi, Bin; Liu, Zheng-xu; Zhan, Jin-shun; Zhan, Kang; Zhao, Guo-qi
Source:
Journal of Zhejiang University 2017 v.18 no.10 pp. 878-885
ISSN:
1673-1581
Subject:
trypsin inhibitors,   etc   ; acetone; agglutinins; animals; beta-amylase; beta-conglycinin; glycinin; isopropyl alcohol; matrix-assisted laser desorption-ionization mass spectrometry; oleosin; phospholipase D; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; soy protein; soybean meal; soybeans; ultrasonics;     Show all 17 Subjects
Abstract:
... The aim of this study was to optimize the conditions for the extraction of low-abundance proteins (LAPs) and the removal of abundant proteins (APs; β-conglycinin and glycinin) from soybean meal. Single factor and orthogonal experiments were designed to determine the effects of four factors (isopropanol concentration, total extraction time, ultrasonic power, and ultrasonic time) on protein concentr ...
DOI:
10.1631/jzus.B1600293
PubMed:
28990378
PubMed Central:
PMC5633772

8. Effect of dehulling, germination and cooking on nutrients, anti-nutrients, fatty acid composition and antioxidant properties in lentil (Lens culinaris)

Author:
Pal, R. S.; Bhartiya, Anuradha; Yadav, Pradhuman; Kant, Lakshmi; Mishra, K. K.; Aditya, J. P.; Pattanayak, A.
Source:
Journal of food science and technology 2017 v.54 no.4 pp. 909-920
ISSN:
0022-1155
Subject:
trypsin inhibitors,   etc   ; Lens culinaris; antinutritional factors; antioxidant activity; antioxidants; catechin; cooking; fatty acid composition; flour; functional foods; gallic acid; germination; hulling; human health; lentils; linoleic acid; linolenic acid; metabolites; nutrients; phytic acid; phytopharmaceuticals; protein content; quercetin; seeds; sugars; tannins;     Show all 26 Subjects
Abstract:
... The changes in chemical composition, antioxidant activity and fatty acid composition of lentil flour after dehulling, germination and cooking of seeds were investigated. Dehulling showed no significant effect on protein content, however, protein content decreased in most of the varieties after germination and cooking. Total soluble sugars (TSS) content increased significantly after dehulling (2.0– ...
DOI:
10.1007/s13197-016-2351-4
PubMed:
28303042
PubMed Central:
PMC5336446

9. Glycopolymer induction of mouse sperm acrosomal exocytosis shows highly cooperative self-antagonism

Author:
Rodolis, Maria T.; Huang, He; Sampson, Nicole S.
Source:
Biochemical and biophysical research communications 2016 v.474 pp. 435-440
ISSN:
0006-291X
Subject:
trypsin inhibitors,   etc   ; acrosome; clinical trials; cryopreservation; dose response; exocytosis; flow cytometry; fluorescence microscopy; fucose; inhibitory concentration 50; lectins; mannose; mice; polymers; propidium; quantitative analysis; screening; soybeans; viability;     Show all 19 Subjects
Abstract:
... Identifying inducers of sperm acrosomal exocytosis (AE) to understand sperm functionality is important for both mechanistic and clinical studies in mammalian fertilization. Epifluorescence microscopy methods, while reproducible, are laborious and incompatible for high throughput screening. Flow cytometry methods are ideal for quantitative measurements on large numbers of samples, yet typically rel ...
DOI:
10.1016/j.bbrc.2016.05.003
PubMed:
27150629
PubMed Central:
PMC4886651

10. Correlation as a Determinant of Configurational Entropy in Supramolecular and Protein Systems

Author:
Fenley, Andrew T.; Killian, Benjamin J.; Hnizdo, Vladimir; Fedorowicz, Adam; Sharp, Dan S.; Gilson, Michael K.
Source:
The Journal of physical chemistry 2014 v.118 no.24 pp. 6447-6455
ISSN:
1520-5207
Subject:
trypsin inhibitors,   etc   ; biochemical compounds; cattle; entropy; models; molecular dynamics; nuclear magnetic resonance spectroscopy;     Show all 7 Subjects
Abstract:
... For biomolecules in solution, changes in configurational entropy are thought to contribute substantially to the free energies of processes like binding and conformational change. In principle, the configurational entropy can be strongly affected by pairwise and higher-order correlations among conformational degrees of freedom. However, the literature offers mixed perspectives regarding the contrib ...
DOI:
10.1021/jp411588b
PubMed:
24702693
PubMed Central:
PMC4067153

11. Cytoprotective effects of urinary trypsin inhibitor on astrocytes injured by sustained compression

Author:
Zhang, Shuang; Yu, Rongguo; Zhang, Yingrui; Chen, Kai
Source:
Molecular biology reports 2014 v.41 no.3 pp. 1311-1316
ISSN:
0301-4851
Subject:
trypsin inhibitors,   etc   ; astrocytes; brain; cell membranes; lactate dehydrogenase; models; protective effect; pups; rats;     Show all 9 Subjects
Abstract:
... Decreased cell membrane integrity is a primary pathological change observed in traumatic brain injury (TBI) that activates a number of complex intercellular and intracellular pathological events, leading to further neural injury. In this paper, we assessed the effects of urinary trypsin inhibitor (UTI) on astrocyte membrane integrity by determining the percentage of lactate dehydrogenase (LDH) rel ...
DOI:
10.1007/s11033-013-2976-6
PubMed:
24385305
PubMed Central:
PMC3933746

12. Entropy–enthalpy transduction caused by conformational shifts can obscure the forces driving protein–ligand binding

Author:
Fenley, Andrew T.; Muddana, Hari S.; Gilson, Michael K.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.49 pp. 20006-20011
ISSN:
0027-8424
Subject:
trypsin inhibitors,   etc   ; calorimetry; cattle; enthalpy; molecular dynamics; variance;     Show all 6 Subjects
Abstract:
... Molecular dynamics simulations of unprecedented duration now can provide new insights into biomolecular mechanisms. Analysis of a 1-ms molecular dynamics simulation of the small protein bovine pancreatic trypsin inhibitor reveals that its main conformations have different thermodynamic profiles and that perturbation of a single geometric variable, such as a torsion angle or interresidue distance, ...
DOI:
10.1073/pnas.1213180109
PubMed:
23150595
PubMed Central:
PMC3523842

13. The boundary structure in the analysis of reversibly interacting systems by sedimentation velocity

Author:
Zhao, Huaying; Balbo, Andrea; Brown, Patrick H.; Schuck, Peter
Source:
Methods 2011 v.54 no.1 pp. 16-30
ISSN:
1046-2023
Subject:
trypsin inhibitors,   etc   ; chemical reactions; computer software; mathematical models; motivation; soybeans;     Show all 6 Subjects
Abstract:
... Sedimentation velocity (SV) experiments of heterogeneous interacting systems exhibit characteristic boundary structures that can usually be very easily recognized and quantified. For slowly interacting systems, the boundaries represent concentrations of macromolecular species sedimenting at different rates, and they can be interpreted directly with population models based solely on the mass action ...
DOI:
10.1016/j.ymeth.2011.01.010
PubMed:
21315155
PubMed Central:
PMC3090504

14. Compensating Effects of Urea and Trimethylamine-N-Oxide on the Heteroassociation of α-Chymotrypsin and Soybean Trypsin Inhibitor

Author:
Wu, Di; Minton, Allen P.
Source:
The Journal of physical chemistry 2013 v.117 no.13 pp. 3554-3559
ISSN:
1520-5207
Subject:
trypsin inhibitors,   etc   ; chymotrypsin; fluorescence; light scattering; pH; physical chemistry; solutes; soybeans; temperature; thermodynamics; urea;     Show all 11 Subjects
Abstract:
... An assay for the determination of the equilibrium constant for heteroassociation of α-chymotrypsin and soybean trypsin inhibitor via fluorescence depolarization is described. Results obtained at neutral pH in saline buffer were consistent with prior determinations via sedimentation equilibrium and static light scattering. The dependence of the association equilibrium constant upon the concentratio ...
DOI:
10.1021/jp4006923
PubMed:
23472887
PubMed Central:
PMC4185429

15. Fatty acid-amino acid conjugates are essential for systemic activation of salicylic acid-induced protein kinase and accumulation of jasmonic acid in Nicotiana attenuata

Author:
Hettenhausen, Christian; Heinrich, Maria; Baldwin, Ian T; Wu, Jianqiang
Source:
BMC plant biology 2014 v.14 no.1 pp. 326
ISSN:
1471-2229
Subject:
trypsin inhibitors,   etc   ; Manduca sexta; Nicotiana attenuata; elicitors; herbivores; jasmonic acid; leaves; metabolites; mitogen-activated protein kinase; tobacco;     Show all 10 Subjects
Abstract:
... BACKGROUND: Herbivory induces the activation of mitogen-activated protein kinases (MAPKs), the accumulation of jasmonates and defensive metabolites in damaged leaves and in distal undamaged leaves. Previous studies mainly focused on individual responses and a limited number of systemic leaves, and more research is needed for a better understanding of how different plant parts respond to herbivory. ...
DOI:
10.1186/s12870-014-0326-z
PubMed:
25430398
PubMed Central:
PMC4263023

16. Efficient Separations of Intact Proteins Using Slip-Flow with Nano-Liquid Chromatography–Mass Spectrometry

Author:
Wu, Zhen; Wei, Bingchuan; Zhang, Ximo; Wirth, Mary J.
Source:
Analytical chemistry 2014 v.86 no.3 pp. 1592-1598
ISSN:
1520-6882
Subject:
trypsin inhibitors,   etc   ; ambient temperature; carbonate dehydratase; mass spectrometry; proteomics; reversed-phase liquid chromatography; ribonucleases; silica; statistical analysis; superoxide dismutase; ubiquitin;     Show all 11 Subjects
Abstract:
... A capillary with a pulled tip, densely packed with silica particles of 0.47 μm in diameter, is shown to provide higher peak capacity and sensitivity in the separation of intact proteins by reversed-phase liquid chromatography–mass spectrometry (LC–MS). For a C18 bonded phase, slip flow gave a 10-fold flow enhancement to allow for stable nanospray with a 4-cm column length. Model proteins were stud ...
DOI:
10.1021/ac403233d
PubMed:
24383398
PubMed Central:
PMC3982985

17. Sprouting characteristics and associated changes in nutritional composition of cowpea (Vigna unguiculata)

Author:
Devi, Chingakham Basanti; Kushwaha, Archana; Kumar, Anil
Source:
Journal of food science and technology 2015 v.52 no.10 pp. 6821-6827
ISSN:
0022-1155
Subject:
trypsin inhibitors,   etc   ; Vigna unguiculata; ascorbic acid; bioavailability; cowpeas; cultivars; dietary fiber; dietary minerals; digestibility; digestible protein; energy; human nutrition; nutrient content; nutrients; phytic acid; soaking; sprouting; temperature;     Show all 18 Subjects
Abstract:
... Cowpea (Vigna unguiculata), is an important arid legume with a good source of energy, protein, vitamins, minerals and dietary fibre. Sprouting of legumes enhances the bioavailability and digestibility of nutrients and therefore plays an important role in human nutrition. Improved varieties of grain cowpea viz. Pant Lobia-1 (PL-1) and Pant Lobia-2 (PL-2) and Pant Lobia-3 (PL-3) were examined for sp ...
DOI:
10.1007/s13197-015-1832-1
PubMed:
26396436
PubMed Central:
PMC4573095

18. BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells

Author:
Dia, Vermont P.; Krishnan, Hari B.
Source:
Scientific Reports 2016 v.6 pp. 1-12
ISSN:
2045-2322
Subject:
trypsin inhibitors,   etc   ; Momordica charantia; anticarcinogenic activity; apoptosis; carcinogenesis; caspase-3; cell cycle; colon; colorectal neoplasms; cyclin-dependent kinase; cytotoxicity; enzyme inhibition; humans; neoplasm cells; peptides; perennials; proteins; soybeans; trypsin;     Show all 19 Subjects
Abstract:
... Momordica charantia is a perennial plant with reported health benefits. BG-4, a novel peptide from Momordica charantia, was isolated, purified and characterized. The trypsin inhibitory activity of BG-4 is 8.6 times higher than purified soybean trypsin inhibitor. The high trypsin inhibitory activity of BG-4 may be responsible for its capability to cause cytotoxicity to HCT-116 and HT-29 human colon ...
Handle:
10113/63140
DOI:
10.1038/srep33532
PubMed:
27628414
PubMed Central:
PMC5024301

19. Stem Nematode Counteracts Plant Resistance of Aphids in Alfalfa, Medicago Sativa

Author:
Ramirez, Ricardo A.; Spears, Lori R.
Source:
Journal of chemical ecology 2014 v.40 no.10 pp. 1099-1109
ISSN:
0098-0331
Subject:
trypsin inhibitors,   etc   ; Acyrthosiphon pisum; Ditylenchus dipsaci; Medicago sativa; alfalfa; cross resistance; greenhouse experimentation; herbivores; insect communities; insects; nematode infections; nutrient availability; peroxidases; plant pathogens; proteins; saponins; signal transduction;     Show all 17 Subjects
Abstract:
... Plants are exploited by a diverse community of insect herbivores and phytopathogens that interact indirectly through plant-mediated interactions. Generally, plants are thought to respond to insects and pathogens through different defensive signaling pathways. As plants are selected for resistance to one phytophagous organism type (insect vs. pathogen) in managed systems, it is not clear how this s ...
DOI:
10.1007/s10886-014-0504-3
PubMed:
25261892
PubMed Central:
PMC4244557

20. Conservation of sequence and function in fertilization of the cortical granule serine protease in echinoderms

Author:
Oulhen, Nathalie; Xu, Dongdong; Wessel, Gary M.
Source:
Biochemical and biophysical research communications 2014 v.450 pp. 1135-1141
ISSN:
0006-291X
Subject:
trypsin inhibitors,   etc   ; Asteroidea; Echinoidea; conserved sequences; cortical granules; eggs; enzyme activity; germplasm conservation; polyspermy; serine proteinases; soybeans; spermatozoa; surface proteins; transcriptome;     Show all 14 Subjects
Abstract:
... Conservation of the cortical granule serine protease during fertilization in echinoderms was tested both functionally in sea stars, and computationally throughout the echinoderm phylum. We find that the inhibitor of serine protease (soybean trypsin inhibitor) effectively blocks proper transition of the sea star fertilization envelope into a protective sperm repellent, whereas inhibitors of the oth ...
DOI:
10.1016/j.bbrc.2014.05.098
PubMed:
24878526
PubMed Central:
PMC4133295

21. Susceptibility of sweetpotato (Ipomoea batatas) peel proteins to digestive enzymes

Author:
Maloney, Katherine P.; Truong, Van-Den; Allen, Jonathan C.
Source:
Food science & nutrition 2014 v.2 no.4 pp. 351-360
ISSN:
2048-7177
Subject:
trypsin inhibitors,   etc   ; Ipomoea batatas; amylases; antioxidants; chymotrypsin; digestion; enzyme inhibition; orange peels; oranges; pepsin; potato protein; protein sweeteners; sporamin; sweet potatoes; trypsin;     Show all 15 Subjects
Abstract:
... Sweet potato proteins have been shown to possess antioxidant and antidiabetic properties in vivo. The ability of a protein to exhibit systemic effects is somewhat unusual as proteins are typically susceptible to digestive enzymes. This study was undertaken to better understand how digestive enzymes affect sweet potato proteins. Two fractions of industrially processed sweet potato peel, containing ...
Handle:
10113/60301
DOI:
10.1002/fsn3.110
PubMed:
25473492
PubMed Central:
PMC4221833
CHORUS:
10.1002/fsn3.110
Chorus Open Access:
10.1002/fsn3.110

22. Decoy Plasminogen Receptor Containing a Selective Kunitz-Inhibitory Domain

Author:
Kumar, Yogesh; Vadivel, Kanagasabai; Schmidt, Amy E.; Ogueli, Godwin I.; Ponnuraj, Sathya M.; Rannulu, Nalaka; Loo, Joseph A.; Bajaj, Madhu S.; Bajaj, S. Paul
Source:
Biochemistry 2014 v.53 no.3 pp. 505-517
ISSN:
1520-4995
Subject:
trypsin inhibitors,   etc   ; active sites; blood; cattle; fibrinolysis; lysine; mice; models; mutants; plasmin; plasminogen; surface plasmon resonance; t-plasminogen activator; thrombin; valine;     Show all 15 Subjects
Abstract:
... Kunitz domain 1 (KD1) of tissue factor pathway inhibitor-2 in which P2′ residue Leu17 (bovine pancreatic trypsin inhibitor numbering) is mutated to Arg selectively inhibits the active site of plasmin with ∼5-fold improved affinity. Thrombin cleavage (24 h extended incubation at a 1:50 enzyme-to-substrate ratio) of the KD1 mutant (Leu17Arg) yielded a smaller molecule containing the intact Kunitz do ...
DOI:
10.1021/bi401584b
PubMed:
24383758
PubMed Central:
PMC3985851

23. A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa

Author:
Glorieux, Sarah; Favoreel, Herman W; Steukers, Lennert; Vandekerckhove, Annelies P; Nauwynck, Hans J
Source:
Veterinary research 2011 v.42 no.1 pp. 58
ISSN:
1297-9716
Subject:
trypsin inhibitors,   etc   ; Suid herpesvirus 1; basement membrane; metalloproteinases; nose; respiratory mucosa; serine proteinases; soybeans; swine;     Show all 9 Subjects
Abstract:
... Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloprotea ...
DOI:
10.1186/1297-9716-42-58
PubMed:
21492440
PubMed Central:
PMC3089791

24. Protease inhibitors as possible pitfalls in proteomic analyses of complex biological samples

Author:
Clifton, James; Huang, Feilei; Rucevic, Marijana; Cao, Lulu; Hixson, Douglas; Josic, Djuro
Source:
Journal of proteomics 2011 v.74 no.7 pp. 935-941
ISSN:
1874-3919
Subject:
trypsin inhibitors,   etc   ; blood proteins; boiling; electrophoresis; fractionation; gels; mass spectrometry; proteolysis; proteomics; spectrometers; trypsin;     Show all 11 Subjects
Abstract:
... Sample preparation, especially protein and peptide fractionation prior to identification by mass spectrometry (MS), is typically applied to reduce sample complexity. The second key element in this process is proteolytic digestion, which is performed most often with trypsin. Optimization of this step is an important factor in order to achieve both speed and better performance of proteomic analysis, ...
DOI:
10.1016/j.jprot.2011.02.010
PubMed:
21333769
PubMed Central:
PMC3107918

25. A cysteine proteinase in the penetration glands of the cercariae of Cotylurus cornutus (Trematoda, Strigeidae)

Author:
Moczoń, Tadeusz
Source:
Parasitology research 2011 v.108 no.3 pp. 639-643
ISSN:
0932-0113
Subject:
trypsin inhibitors,   etc   ; Cotylurus; cathepsin B; cercariae; enzyme activity; gelatin; metal ions; pH; soybeans;     Show all 9 Subjects
Abstract:
... A cysteine proteinase from the penetration glands of Cotylurus cornutus cercariae was examined with histochemical and biochemical methods. The enzyme hydrolyzed gelatin, azocoll, azocasein, azoalbumin, N-blocked-L-arginine-4-methoxy-2-naphthylamide, and N-blocked-p-nitroanilide, but did not degrade elastin. The metal ion complexane ethylenediamine tetraacetate and the thiol-reducing compound dithi ...
DOI:
10.1007/s00436-010-2108-3
PubMed:
20981445
PubMed Central:
PMC3040816

26. Production of pure protein antibodies and development of immunoassays to detect Ara h 3 levels in peanut varieties

Author:
Hurlburt, Barry K.; Schmitt, David; Isleib, Thomas G.; Cheng, Hsiaopo; Garvey, Cathryn; Koenig, Robbin L.; Maleki, Soheila J.
Source:
International journal of food science & technology 2011 v.460 pp. 1477
ISSN:
0950-5423
Subject:
trypsin inhibitors,   etc   ; Arachis hypogaea; allergenicity; antibodies; cultivars; epitopes; food allergies; foods; immunoassays; peanuts; protein folding; proteins;     Show all 12 Subjects
Abstract:
... Peanuts are one of the most allergenic foods and are widespread in western food products. Therefore, there has been intense research into the allergic nature of the proteins involved. Ara h 3 is one of three immunodominant allergenic proteins. It is a 60-kDa protein, which forms following cleavage of the preprotein and association of the resultant 40- and 20-kDa subunits. The large subunit has bee ...
Handle:
10113/55608
DOI:
10.1111/j.1365-2621.2011.02645.x

27. Kaulath, a new fungal fermented food from horse gram

Author:
Dwivedi, Minakshee; Vasantha, K . Y.; Sreerama, Y. N.; Haware, Devendra J.; Singh, R. P.; Sattur, A .P.
Source:
Journal of food science and technology 2015 v.52 no.12 pp. 8371-8376
ISSN:
0022-1155
Subject:
trypsin inhibitors,   etc   ; Macrotyloma uniflorum; Penicillium; alpha-glucosidase; antinutritional factors; body weight; calcium; diet; fermented foods; free radical scavengers; functional foods; fungi; histopathology; kidneys; lipid content; liver; mortality; phytic acid; potassium; rats; sodium; solid state fermentation; toxicity; triacylglycerol lipase;     Show all 24 Subjects
Abstract:
... Horse gram (Macrotyloma uniflorum) is used in the traditional method for treatmentof several health complications. It is also known that fermentation of such substrates yields a number of compounds that enhance the overall activities against several disease states. Solid state fermentation of horse gram using Penicillium camemberti showed an inhibition of pancreatic lipase and alpha glucosidase ac ...
DOI:
10.1007/s13197-015-1887-z
PubMed:
26604417
PubMed Central:
PMC4648863

28. Effect of processing methods on compositional evaluation of underutilized legume, Parkia roxburghii G. Don (yongchak) seeds

Author:
Sathya, Arumugam; Siddhuraju, Perumal
Source:
Journal of food science and technology 2015 v.52 no.10 pp. 6157-6169
ISSN:
0022-1155
Subject:
trypsin inhibitors,   etc   ; Parkia timoriana; amino acid composition; antinutritional factors; autoclaving; chymotrypsin; digestibility; fatty acids; fermentation; food processing; food security; lectins; legumes; lipid content; minerals; phytic acid; proanthocyanidins; protein content; saponins; seeds; soaking; sodium bicarbonate; starch; sugars; tannins; trees;     Show all 26 Subjects
Abstract:
... The present study has been undertaken to analyze the effect of various processing methods like (i) soaking followed by autoclaving with (a) ash, (b) sodium bicarbonate, (c) sugar and (d) water; (ii) dry heating and (iii) fermentation on nutritional and antinutritional components of under-utilized tree legume Parkia roxburghii. The applied methods were found to enhance the protein (15–36 %) and lip ...
DOI:
10.1007/s13197-015-1732-4
PubMed:
26396363
PubMed Central:
PMC4573156

29. Changes in nutrient and antinutrient composition of Vigna racemosa flour in open and controlled fermentation

Author:
Difo, V. H.; Onyike, E.; Ameh, D. A.; Njoku, G. C.; Ndidi, U. S.
Source:
Journal of food science and technology 2015 v.52 no.9 pp. 6043-6048
ISSN:
0022-1155
Subject:
trypsin inhibitors,   etc   ; Aspergillus niger; Vigna; alkaloids; antinutritional factors; ash content; calcium; crude fiber; fermentation; flour; hydrogen cyanide; iron; lectins; lipids; magnesium; microorganisms; oxalates; phytic acid; protein content; proximate composition; raffinose; sodium; stachyose; water content; zinc;     Show all 25 Subjects
Abstract:
... This study was conducted to investigate the effect of open and controlled fermentation on the proximate composition, mineral elements, antinutritional factors and flatulence-causing oligosaccharides in Vigna racemosa. The open fermentation was carried out using the microorganisms present in the atmosphere while the controlled fermentation was carried out using Aspergillus niger as a starter. The p ...
DOI:
10.1007/s13197-014-1637-7
PubMed:
26345026
PubMed Central:
PMC4554638

30. Impact of dehulling and germination on nutrients, antinutrients, and antioxidant properties in horsegram

Author:
Pal, R. S.; Bhartiya, Anuradha; ArunKumar, R.; Kant, Lakshmi; Aditya, J. P.; Bisht, J. K.
Source:
Journal of food science and technology 2016 v.53 no.1 pp. 337-347
ISSN:
0022-1155
Subject:
trypsin inhibitors,   etc   ; 2,2-diphenyl-1-picrylhydrazyl; Macrotyloma uniflorum; absorption; antinutritional factors; antioxidant activity; antioxidants; bulk density; calcium; copper; flavonoids; flour; functional properties; germination; hulling; iron; lipids; minerals; nutrients; nutritive value; oils; oxalic acid; phytic acid; phytopharmaceuticals; polyphenols; principal component analysis; protein content; sugars; zinc;     Show all 29 Subjects
Abstract:
... The changes in chemical composition, antioxidant activity and minerals content of horse gram seed after dehulling and germination of 12 advance lines were investigated. Dehulled samples had a higher protein content compared with the raw and germinated. Total soluble sugars (TSS) content increased significantly (p ≤ 0.05) after dehulling (29.31 %) and germination (98.73 %) whereas, the total lipids ...
DOI:
10.1007/s13197-015-2037-3
PubMed:
26787953
PubMed Central:
PMC4711462

31. Seed Transmission Rates of Bean pod mottle virus and Soybean mosaic virus in Soybean May Be Affected by Mixed Infection or Expression of the Kunitz Trypsin Inhibitor

Author:
Nam, Moon; Bai, Hanhong; Hammond, John; Domier, Leslie L.; Youn, Young-Nam; Lee, Bong-Choon; Lim, Hyoun-Sub
Source:
Research in Plant Disease 2013 v.19 no.2 pp. 114
ISSN:
2233-9191
Subject:
trypsin inhibitors,   etc   ; Bean pod mottle virus; Soybean mosaic virus; digestion; disease transmission; gene expression; genes; insects; mixed infection; mutation; plant viruses; seed-borne diseases; trypsin;     Show all 13 Subjects
Abstract:
... To facilitate their spread, plant viruses have developed several methods for dispersal including insect and seed transmission. While insect transmission requires virus stability against insect digestion, seed-transmitted viruses have to overcome barriers to entry into embryos. Bean pod mottle virus (BPMV) is transmitted through seed at levels typically below 0.1%, but co-infection with Soybean mos ...
Handle:
10113/58414
DOI:
10.5423/RPD.2013.19.2.114

32. In vivo biosynthesis of an Ala-scan library based on the cyclic peptide SFTI-1

Author:
Austin, Jeffrey; Kimura, Richard H.; Woo, Youn-Hi; Camarero, Julio A.
Source:
Amino acids 2010 v.38 no.5 pp. 1313-1322
ISSN:
0939-4451
Subject:
trypsin inhibitors,   etc   ; Escherichia coli; Helianthus annuus; biosynthesis; directed evolution; disulfide bonds; mutants; peptide libraries; polypeptides; screening;     Show all 10 Subjects
Abstract:
... We present the in vivo biosynthesis of wild-type sunflower trypsin inhibitor 1 (SFTI-1) inside E. coli cells using an intramolecular native chemical ligation in combination with a modified protein splicing unit. SFTI-1 is a small backbone cyclized polypeptide with a single disulfide bridge. A small library containing multiple Ala mutants was also biosynthesized and its activity was assayed using a ...
DOI:
10.1007/s00726-009-0338-4
PubMed:
19685144
PubMed Central:
PMC2936557

33. Nicotiana attenuata LECTIN RECEPTOR KINASE1 Suppresses the Insect-Mediated Inhibition of Induced Defense Responses during Manduca sexta Herbivory

Author:
Gilardoni, Paola A.; Hettenhausen, Christian; Baldwin, Ian T.; Bonaventure, Gustavo
Source:
The plant cell 2011 v.23 no.9 pp. 3512-3532
ISSN:
1040-4651
Subject:
trypsin inhibitors,   etc   ; Manduca sexta; Nicotiana; RNA interference; genes; herbivores; jasmonic acid; larvae; nicotine; receptors; salicylic acid; secretion;     Show all 12 Subjects
Abstract:
... Nicotiana attenuata has the capacity to respond specifically to herbivory by its natural herbivore, Manduca sexta, through the perception of elicitors in larval oral secretions. We demonstrate that Lectin receptor kinase 1 (LecRK1) functions during M. sexta herbivory to suppress the insect-mediated inhibition of jasmonic acid (JA)–induced defense responses. Gene function analysis performed by redu ...
DOI:
10.1105/tpc.111.088229
PubMed:
21926334
PubMed Central:
PMC3203443

34. Structure of a serine protease poised to resynthesize a peptide bond

Author:
Zakharova, Elena; Horvath, Martin P.; Goldenberg, David P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.27 pp. 11034-11039
ISSN:
0027-8424
Subject:
trypsin inhibitors,   etc   ; active sites; cattle; crystal structure; enzyme substrates; hydrogen bonding; hydrolysis; trypsin;     Show all 8 Subjects
Abstract:
... The serine proteases are among the most thoroughly studied enzymes, and numerous crystal structures representing the enzyme-substrate complex and intermediates in the hydrolysis reactions have been reported. Some aspects of the catalytic mechanism remain controversial, however, especially the role of conformational changes in the reaction. We describe here a high-resolution (1.46 Å) crystal struct ...
DOI:
10.1073/pnas.0902463106
PubMed:
19549826
PubMed Central:
PMC2708782

35. NaJAZh Regulates a Subset of Defense Responses against Herbivores and Spontaneous Leaf Necrosis in Nicotiana attenuata Plants

Author:
Oh, Youngjoo; Baldwin, Ian T.; Gális, Ivan
Source:
Plant physiology 2012 v.159 no.2 pp. 769-788
ISSN:
0032-0889
Subject:
trypsin inhibitors,   etc   ; Nicotiana; RNA interference; genes; herbivores; hydrogen peroxide; jasmonic acid; leaves; necrosis; nicotine; plant development; planting; proteins; roots; salicylic acid; tobacco; volatile organic compounds;     Show all 17 Subjects
Abstract:
... The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most JAZ genes showed strong expression in the elicited leaves, but NaJAZg was mainly ...
DOI:
10.1104/pp.112.193771
PubMed:
22496510
PubMed Central:
PMC3375940

36. Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation

Author:
Foley, J.H.; Butenas, S.; Mann, K.G.; Brummel-Ziedins, K.E.
Source:
Analytical biochemistry 2012 v.422 no.1 pp. 46-51
ISSN:
0003-2697
Subject:
trypsin inhibitors,   etc   ; blood; blood coagulation; coagulation; corn; degradation; dynamics; fibrin; fibrinolysis; males; mechanical properties; platelet aggregation; quality control; t-plasminogen activator; viscoelasticity;     Show all 15 Subjects
Abstract:
... Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from ...
DOI:
10.1016/j.ab.2011.12.036
PubMed:
22266209
PubMed Central:
PMC3778664

37. Alpha-1 antitrypsin reduces ovariectomy-induced bone loss in mice

Author:
Cao, Jay J.; Gregoire, Brian R.; Sun, Li; Song, Sihong
Source:
Annals of the New York Academy of Sciences 2012 v.1240 pp. 1
ISSN:
0077-8923
Subject:
trypsin inhibitors,   etc   ; acid phosphatase; anti-inflammatory activity; blood serum; bone marrow; bone resorption; calcitonin; cytokines; estrogens; leptin; mice; osteocalcin; osteoclasts; ovariectomy; phosphates; receptors; tibia; tissue weight; uterus;     Show all 19 Subjects
Abstract:
... Proinflammatory cytokines are primary mediators of bone loss in estrogen deficiency. This study determined whether alpha-1 antitrypsin (AAT), a multifunctional protein with proteinase inhibitor and anti-inflammatory activities, mitigates bone loss induced by estrogen deficiency. Mice were either sham-operated or ovariectomized and injected with either AAT or phosphate buffered saline (PBS). Ovarie ...
Handle:
10113/56919
DOI:
10.111/j.1749-6632.2011.06370.x

38. Variation in Antiherbivore Defense Responses in Synthetic Nicotiana Allopolyploids Correlates with Changes in Uniparental Patterns of Gene Expression

Author:
Anssour, Samir; Baldwin, Ian T.
Source:
Plant physiology 2010 v.153 no.4 pp. 1907-1918
ISSN:
0032-0889
Subject:
trypsin inhibitors,   etc   ; Manduca sexta; Nicotiana; adaptive radiation; allopolyploidy; autopolyploidy; gene expression; gene expression regulation; jasmonic acid; methyl jasmonate; salicylic acid;     Show all 11 Subjects
Abstract:
... We examined the expression of Nicotiana attenuata (Na) and Nicotiana obtusifolia (No) herbivore-induced genes in synthetic autopolyploids (NaT and NoT) and five independent allopolyploid Nicotiana x obtusiata (Nxo) lines to understand how the expression of genes regulating complex polygenetic defense traits is altered in the early stages of allopolyploid hybridization. In Na, applying Manduca sext ...
DOI:
10.1104/pp.110.156786
PubMed:
20525855
PubMed Central:
PMC2923876

39. Crystal structure of an extensively simplified variant of bovine pancreatic trypsin inhibitor in which over one-third of the residues are alanines

Author:
Islam, Mohammad Monirul; Sohya, Shihori; Noguchi, Keiichi; Yohda, Masafumi; Kuroda, Yutaka
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.40 pp. 15334-15339
ISSN:
0027-8424
Subject:
trypsin inhibitors,   etc   ; alanine; cattle; crystal structure; disulfide bonds; hydrophobicity; proteins; surface area; trypsin;     Show all 9 Subjects
Abstract:
... We report the high-resolution crystal structures of an extensively simplified variant of bovine pancreatic trypsin inhibitor containing 20 alanines (BPTI-20st) and a reference single-disulfide-bonded variant (BPTI-[5,55]st) at, respectively, 1.39 and 1.09 Å resolutions. The sequence was simplified based on the results of an alanine scanning experiment, as reported previously. The effects of the mu ...
DOI:
10.1073/pnas.0802699105
PubMed:
18829434
PubMed Central:
PMC2563086

40. Effects of Clostridium perfringens Beta-Toxin on the Rabbit Small Intestine and Colon

Author:
Vidal, Jorge E.; McClane, Bruce A.; Saputo, Juliann; Parker, Jaquelyn; Uzal, Francisco A.
Source:
Infection and immunity 2008 v.76 no.10 pp. 4396-4404
ISSN:
0019-9567
Subject:
trypsin inhibitors,   etc   ; Clostridium perfringens; colon; duodenum; humans; ileum; jejunum; livestock; models; necrotic enteritis; rabbits; trypsin;     Show all 12 Subjects
Abstract:
... Clostridium perfringens type B and type C isolates, which produce beta-toxin (CPB), cause fatal diseases originating in the intestines of humans or livestock. Our previous studies demonstrated that CPB is necessary for type C isolate CN3685 to cause bloody necrotic enteritis in a rabbit ileal loop model and also showed that purified CPB, in the presence of trypsin inhibitor (TI), can reproduce typ ...
DOI:
10.1128/IAI.00547-08
PubMed:
18625730
PubMed Central:
PMC2546809

41. Dissimilar Properties of Two Recombinant Lactobacillus acidophilus Strains Displaying Salmonella FliC with Different Anchoring Motifs

Author:
Kajikawa, Akinobu; Nordone, Shila K.; Zhang, Lin; Stoeker, Laura L.; LaVoy, Alora S.; Klaenhammer, Todd R.; Dean, Gregg A.
Source:
Applied and environmental microbiology 2011 v.77 no.18 pp. 6587-6596
ISSN:
0099-2240
Subject:
trypsin inhibitors,   etc   ; Lactobacillus acidophilus; Salmonella; bicarbonates; binding proteins; buffers; cell walls; dendritic cells; digestive juices; enzymes; flagellin; humans; immunization; juices; mucus; recombinant vaccines; soybeans; surface antigens; viability;     Show all 19 Subjects
Abstract:
... Display of heterologous antigens on the cell surface is considered a useful technique for vaccine delivery by recombinant lactobacilli. In this study, two recombinant Lactobacillus acidophilus derivatives displaying Salmonella flagellin (FliC) were constructed using different anchor motifs. In one instance, the FliC protein was fused to the C-terminal region of a cell envelope proteinase (PrtP) an ...
DOI:
10.1128/AEM.05153-11
PubMed:
21784918
PubMed Central:
PMC3187123

42. Ectopic Expression of AtJMT in Nicotiana attenuata: Creating a Metabolic Sink Has Tissue-Specific Consequences for the Jasmonate Metabolic Network and Silences Downstream Gene Expression

Author:
Stitz, Michael; Gase, Klaus; Baldwin, Ian T.; Gaquerel, Emmanuel
Source:
Plant physiology 2011 v.157 no.1 pp. 341-354
ISSN:
0032-0889
Subject:
trypsin inhibitors,   etc   ; Arabidopsis thaliana; Manduca sexta; Nicotiana; biosynthesis; gene expression; gene silencing; genes; jasmonic acid; methyl jasmonate; petioles; secretion; transgenic plants;     Show all 13 Subjects
Abstract:
... To create a metabolic sink in the jasmonic acid (JA) pathway, we generated transgenic Nicotiana attenuata lines ectopically expressing Arabidopsis (Arabidopsis thaliana) jasmonic acid O-methyltransferase (35S-jmt) and additionally silenced in other lines the N. attenuata methyl jasmonate esterase (35S-jmt/ir-mje) to reduce the deesterification of methyl jasmonate (MeJA). Basal jasmonate levels did ...
DOI:
10.1104/pp.111.178582
PubMed:
21753114
PubMed Central:
PMC3165883

43. Two mitogen-activated protein kinase kinases, MKK1 and MEK2, are involved in wounding- and specialist lepidopteran herbivore Manduca sexta-induced responses in Nicotiana attenuata

Author:
Heinrich, Maria; Baldwin, Ian T.; Wu, Jianqiang
Source:
Journal of experimental botany 2011 v.62 no.12 pp. 4355-4365
ISSN:
0022-0957
Subject:
trypsin inhibitors,   etc   ; Manduca sexta; Nicotiana; ethylene; gene silencing; genes; herbivores; jasmonic acid; mitogen-activated protein kinase; phosphorylation; proteins; secondary metabolites; secretion; wild plants;     Show all 14 Subjects
Abstract:
... In a wild tobacco plant, Nicotiana attenuata, two mitogen-activated protein kinases (MAPKs), salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), play central roles in modulating herbivory-induced phytohormone and anti-herbivore secondary metabolites. However, the identities of their upstream MAPK kinases (MAPKKs) were elusive. Ectopic overexpression studies in N. ...
DOI:
10.1093/jxb/err162
PubMed:
21610019
PubMed Central:
PMC3153688

44. BAK1 regulates the accumulation of jasmonic acid and the levels of trypsin proteinase inhibitors in Nicotiana attenuata's responses to herbivory

Author:
Yang, Da-Hai; Hettenhausen, Christian; Baldwin, Ian T.; Wu, Jianqiang
Source:
Journal of experimental botany 2011 v.62 no.2 pp. 641-652
ISSN:
0022-0957
Subject:
trypsin inhibitors,   etc   ; Manduca sexta; Nicotiana; enzyme activity; flagellin; gene silencing; herbivores; jars; jasmonic acid; larvae; methyl jasmonate; mitogen-activated protein kinase; pathogens; plant damage; receptors; secondary metabolites; secretion;     Show all 17 Subjects
Abstract:
... BAK1 is a co-receptor of brassinosteroid (BR) receptor BRI1, and plays a well-characterized role in BR signalling. BAK1 also physically interacts with the flagellin receptor FLS2 and regulates pathogen resistance. The role of BAK1 in mediating Nicotiana attenuata's resistance responses to its specialist herbivore, Manduca sexta, was examined here. A virus-induced gene-silencing system was used to ...
DOI:
10.1093/jxb/erq298
PubMed:
20937731
PubMed Central:
PMC3003809

45. Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey

Author:
Hsu, Chen-Tran; Liao, De-Chih; Wu, Fu-Hui; Liu, Nien-Tze; Shen, Shu-Chen; Chou, Shu-Jen; Tung, Shu-Yun; Yang, Chang-Hsien; Chan, Ming-Tsair; Lin, Choun-Sea
Source:
BMC Plant biology 2011 v.11
ISSN:
1471-2229
Subject:
trypsin inhibitors,   etc   ; Arabidopsis thaliana; Oncidium; bacterial artificial chromosomes; cDNA libraries; clones; complementary DNA; flowers; gene expression; leaves; microarray technology; molecular biology; promoter regions; proteins; reporter genes;     Show all 15 Subjects
Abstract:
... Background Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However, model plants, such as Arabidopsis thaliana do not contain all plant genes, and agronomic and horticulturally important genera and species must be individually studied. Results Several molecular biology tools were used to isolate flower-specific gene promoters from Oncid ...
DOI:
10.1186/1471-2229-11-60
PubMed:
21473751
PubMed Central:
PMC3079641

46. Identification of genes required for Cf-dependent hypersensitive cell death by combined proteomic and RNA interfering analyses

Author:
Xu, Qiu-Fang; Cheng, Wei-Shun; Li, Shuang-Sheng; Li, Wen; Zhang, Zhi-Xin; Xu, You-Ping; Zhou, Xue-Ping; Cai, Xin-Zhong
Source:
Journal of experimental botany 2012 v.63 no.7 pp. 2421-2435
ISSN:
0022-0957
Subject:
trypsin inhibitors,   etc   ; Fulvia fulva; G-proteins; Nicotiana benthamiana; RNA; RNA interference; Xanthomonas oryzae; aspartate-ammonia ligase; cell death; disease resistance; genes; mitogen-activated protein kinase; pathogens; plant diseases and disorders; tomatoes;     Show all 15 Subjects
Abstract:
... Identification of hypersensitive cell death (HCD) regulators is essential to dissect the molecular mechanisms underlying plant disease resistance. In this study, combined proteomic and RNA interfering (RNAi) analyses were employed to identify genes required for the HCD conferred by the tomato resistance gene Cf-4 and the Cladosporium fulvum avirulence gene Avr4. Forty-nine proteins differentially ...
DOI:
10.1093/jxb/err397
PubMed:
22275387
PubMed Central:
PMC3346213

47. Silencing NaTPI Expression Increases Nectar Germin, Nectarins, and Hydrogen Peroxide Levels and Inhibits Nectar Removal from Plants in Nature

Author:
Bezzi, Siham; Kessler, Danny; Diezel, Celia; Muck, Alexander; Anssour, Samir; Baldwin, Ian T.
Source:
Plant physiology 2010 v.152 no.4 pp. 2232-2242
ISSN:
0032-0889
Subject:
trypsin inhibitors,   etc   ; Nicotiana; anthers; attractants; benzaldehyde; chemistry; emissions; field experimentation; glucose oxidase; hydrogen peroxide; indigenous species; insects; nectar; nicotine; plants; pollinators; proteins; proteomics; sugars;     Show all 19 Subjects
Abstract:
... Native flower visitors removed less nectar from trypsin proteinase inhibitor (TPI)-silenced Nicotiana attenuata plants (ir-pi) than from wild-type plants in four field seasons of releases, even when the nectar repellant, nicotine, was also silenced. Analysis of floral chemistry revealed no differences in the emission of the floral attractants benzylacetone and benzaldehyde or in the concentrations ...
DOI:
10.1104/pp.109.151753
PubMed:
20190094
PubMed Central:
PMC2850041

48. Discovery and Characterization of Proteins Associated with Aflatoxin-Resistance: Evaluating Their Potential as Breeding Markers

Author:
Brown, Robert L.; Chen, Zhi-Yuan; Warburton, Marilyn; Luo, Meng; Menkir, Abebe; Fakhoury, Ahmad; Bhatnagar, Deepak
Source:
Toxins 2010 v.2 no.4 pp. 919
Subject:
trypsin inhibitors,   etc   ; Zea mays; corn; plant proteins; seeds; disease resistance; fungal diseases of plants; aflatoxins; plant breeding; proteome; proteomics; pathogenesis-related proteins;     Show all 12 Subjects
Abstract:
... Host resistance has become a viable approach to eliminating aflatoxin contamination of maize since the discovery of several maize lines with natural resistance. However, to derive commercial benefit from this resistance and develop lines that can aid growers, markers need to be identified to facilitate the transfer of resistance into commercially useful genetic backgrounds without transfer of unwa ...
Handle:
10113/43609
DOI:
10.3390/toxins2040919
PubMed:
22069617
PubMed Central:
PMC3153200

49. Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells

Author:
Sherwood, Mark W.; Prior, Ian A.; Voronina, Svetlana G.; Barrow, Stephanie L.; Woodsmith, Jonathan D.; Gerasimenko, Oleg V.; Petersen, Ole H.; Tepikin, Alexei V.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.13 pp. 5674-5679
ISSN:
0027-8424
Subject:
trypsin inhibitors,   etc   ; acinar cells; calcium; cholecystokinin; dextran; ethylene glycol tetraacetic acid; fluorescein; fluorescence; models; pH; pancreatitis; tracer techniques; trypsinogen; vacuoles;     Show all 14 Subjects
Abstract:
... The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute pancreatitis: supramaximal stimulati ...
DOI:
10.1073/pnas.0700951104
PubMed:
17363470
PubMed Central:
PMC1838486

50. Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice

Author:
Du, Bo; Zhang, Weilin; Liu, Bingfang; Hu, Jing; Wei, Zhe; Shi, Zhenying; He, Ruifeng; Zhu, Lili; Chen, Rongzhi; Han, Bin; He, Guangcun
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.52 pp. 22163-22168
ISSN:
0027-8424
Subject:
trypsin inhibitors,   etc   ; Oryza sativa; rice; Nilaparvata lugens; insect pests; pest resistance; genes; plant proteins; gene expression; messenger RNA; vascular bundles; salicylic acid; signal transduction; callose; phloem; antibiosis;     Show all 16 Subjects
Abstract:
... Planthoppers are highly destructive pests in crop production worldwide. Brown planthopper (BPH) causes the most serious damage of the rice crop globally among all rice pests. Growing resistant varieties is the most effective and environment-friendly strategy for protecting the crop from BPH. More than 19 BPH-resistance genes have been reported and used to various extents in rice breeding and produ ...
DOI:
10.1073/pnas.0912139106
PubMed:
20018701
PubMed Central:
PMC2793316