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- Penalver, J., et al. Show all 4 Authors
- Applied and environmental microbiology 2000 v.66 no.5 pp. 2071-2078
- polymerase chain reaction; plant diseases and disorders
- ... A novel method, which involves a nested PCR in a single closed tube, was developed for the sensitive detection of Erwinia amylovora in plant material. The external and internal primer pairs used had different annealing temperatures and directed the amplification of a specific DNA fragment from plasmid pEA29. The procedure involved two consecutive PCRs, the first of which was performed at a higher ...
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