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1. H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum

Author:
Tappel, Ryan,   et al.   ; Valgepea, Kaspar; de Souza Pinto Lemgruber, Renato; Abdalla, Tanus; Binos, Steve; Takemori, Nobuaki; Takemori, Ayako; Tanaka, Yuki; Köpke, Michael; Simpson, Séan Dennis; Nielsen, Lars Keld; Marcellin, Esteban;     Show all 12 Authors
Source:
Biotechnology for biofuels 2018 v.11 no.1 pp. 55
ISSN:
1754-6834
Subject:
Clostridium autoethanogenum; acetates; biomass; carbon; carbon dioxide; carbon monoxide; energy; energy conservation; ethanol; fermentation; formates; hydrogen; metabolomics; models; oxidation; proteome; proteomics; synthesis gas
Abstract:
... BACKGROUND: The global demand for affordable carbon has never been stronger, and there is an imperative in many industrial processes to use waste streams to make products. Gas-fermenting acetogens offer a potential solution and several commercial gas fermentation plants are currently under construction. As energy limits acetogen metabolism, supply of H₂ should diminish substrate loss to CO₂ and fa ...
DOI:
10.1186/s13068-018-1052-9
PubMed:
29507607
PubMed Central:
PMC5831606

2. Development of a New Strategy for Production of Medium-Chain-Length Polyhydroxyalkanoates by Recombinant Escherichia coli via Inexpensive Non-Fatty Acid Feedstocks

Author:
Tappel, Ryan C.,   et al.   ; Wang, Qin; Zhu, Chengjun; Nomura, Christopher T.;     Show all 4 Authors
Source:
Applied and environmental microbiology 2012 v.78 no.2 pp. 519-527
ISSN:
0099-2240
Subject:
Escherichia coli; Pseudomonas putida; biosynthesis; carbon; feedstocks; gene expression; genes; glucose; glycerol; polyhydroxyalkanoates; polymerase chain reaction; transport proteins
Abstract:
... Pseudomonas putida KT2440 is capable of producing medium-chain-length polyhydroxyalkanoates (MCL-PHAs) when grown on unrelated carbon sources during nutrient limitation. Transcription levels of genes putatively involved in PHA biosynthesis were assessed by quantitative real-time PCR (qRT-PCR) in P. putida grown on glycerol as a sole carbon source. The results showed that two genes, phaG and the PP ...
DOI:
10.1128/AEM.07020-11
PubMed:
22101037
PubMed Central:
PMC3255744

3. Glycerine and levulinic acid: Renewable co-substrates for the fermentative synthesis of short-chain poly(hydroxyalkanoate) biopolymers

Author:
Tappel, Ryan C.,   et al.   ; Ashby, Richard D.; Solaiman, Daniel K.Y.; Strahan, Gary D.; Zhu, Chengjun; Nomura, Christopher T.;     Show all 6 Authors
Source:
Bioresource technology 2012 v.118 pp. 272
ISSN:
0960-8524
Subject:
Pseudomonas oleovorans; batch fermentation; biodiesel; biopolymers; composite polymers; coproducts; levulinic acid; molecular weight
Abstract:
... Glycerine (a biodiesel co-product) and levulinic acid (a pulp and paper co-product) were used as co-substrates for the fermentative synthesis of short-chain polyhydroxyalkanoate (sc-PHA) biopolymers with tunable monomer and molecular weight characteristics. Pseudomonas oleovorans NRRL B-14682 utilized glycerine alone to produce poly(3-hydroxybutyrate) (PHB). When levulinic acid was added to the me ...
Handle:
10113/54532
DOI:
10.1016/j.biortech.2012.05.092