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... We describe a novel plant transformation technique, termed "agrolistic," that combines the advantages of the Agrobacterium transformation system with the high efficiency of biolistic DNA delivery. Agrolistic transformation allows integration of the gene of interest without undesired vector sequence. The virulence genes virD1 and rirD2 from Agrobacterium tumefaciens that are required in bacteria fo ...
... We investigated the fine-scale population structure of the "Candidatus Accumulibacter" lineage in enhanced biological phosphorus removal (EBPR) systems using the polyphosphate kinase 1 gene (ppk1) as a genetic marker. We retrieved fragments of "Candidatus Accumulibacter" 16S rRNA and ppk1 genes from one laboratory-scale and several full-scale EBPR systems. Phylogenies reconstructed using 16S rRNA ...
... The order Rickettsiales (Alphaproteobacteria) is a well-known group containing obligate endocellular prokaryotes. The order encompasses three families (Rickettsiaceae, Anaplasmataceae, and Holosporaceae) and a fourth, family-level cluster, which includes only one candidate species, "Candidatus Midichloria mitochondrii," as well as several unnamed bacterial symbionts. The broad host range exhibited ...
... Pointed, rod-shaped bacteria colonizing the cuticular surface of the hindgut of the terrestrial isopod crustacean Porcellio scaber (Crustacea: Isopoda) were investigated by comparative 16S rRNA gene sequence analysis and electron microscopy. The results of phylogenetic analysis, and the absence of a cell wall, affiliated these bacteria with the class Mollicutes, within which they represent a novel ...
... Here we investigated the bacterial endosymbionts of weevils of the genus CURCULIO: From all four species of Curculio weevils examined, a novel group of bacterial gene sequences were consistently identified. Molecular phylogenetic analyses demonstrated that the sequences formed a distinct clade in the Gammaproteobacteria, which was not related to previously known groups of weevil endosymbionts such ...
... Both Helicobacter pylori and "Candidatus Helicobacter heilmannii" infections are associated with peptic ulcers, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphomas. However, good animal models of H. pylori clinical diseases are rare. In this study, we aimed to establish an animal model of "Candidatus Helicobacter heilmannii" gastric MALT lymphoma. We used a ureas ...
... Zoothamnium niveum is a giant, colonial marine ciliate from sulfide-rich habitats obligatorily covered with chemoautotrophic, sulfide-oxidizing bacteria which appear as coccoid rods and rods with a series of intermediate shapes. Comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization showed that the ectosymbiont of Z. niveum belongs to only one pleomorphic phylotype. The ...
... A phytoplasma was detected in naturally diseased 'Chardonnay' grapevines exhibiting symptoms of Australian grapevine yellows disease. The use of PCR designed to amplify phytoplasma DNA resulted in detection of phytoplasma DNA in all of the diseased plants examined; no phytoplasma DNA was detected in healthy seedling grapevines. The collective restriction fragment length polymorphism (RFLP) pattern ...
microalgae; population dynamics; sexual reproduction; viruses; infection; Phycodnaviridae; disease resistance; diploidy; haploidy
Abstract:
... The coccolithophore Emiliania huxleyi is one of the most successful eukaryotes in modern oceans. The two phases in its haplodiploid life cycle exhibit radically different phenotypes. The diploid calcified phase forms extensive blooms, which profoundly impact global biogeochemical equilibria. By contrast, the ecological role of the noncalcified haploid phase has been completely overlooked. Giant ph ...
... Failure to recognize that many standard control rats and mice used in biomedical research are sedentary, obese, glucose intolerant, and on a trajectory to premature death may confound data interpretation and outcomes of human studies. Fundamental aspects of cellular physiology, vulnerability to oxidative stress, inflammation, and associated diseases are among the many biological processes affected ...
Dehalococcoides; bacteria; biphenyl; chlorine; dechlorination; estuaries; gas chromatography; polychlorinated biphenyls; Hudson River; New York
Abstract:
... "Dehalococcoides" sp. strain CBDB1 in pure culture dechlorinates a wide range of PCB congeners with three to eight chlorine substituents. Congener-specific high-resolution gas chromatography revealed that CBDB1 extensively dechlorinated both Aroclor 1248 and Aroclor 1260 after four months of incubation. For example, 16 congeners comprising 67.3% of the total PCBs in Aroclor 1260 were decreased by ...
... More than 5,000 measurements from 1,943 plant species were used to explore the scaling relationships among the foliar surface area and the dry, water, and nitrogen/phosphorus mass of mature individual leaves. Although they differed statistically, the exponents for the relationships among these variables were numerically similar among six species groups (ferns, graminoids, forbs, shrubs, trees, and ...
... Symbiotic bacteria residing in the hindgut chambers of scarab beetle larvae may be useful in paratransgenic approaches to reduce larval root-feeding activities on agricultural crops. We compared the bacterial community profiles associated with the hindgut walls of individual Dermolepida albohirtum third-instar larvae over 2 years and those associated with their plant root food source among differe ...
intestinal microorganisms; ribosomal RNA; hindgut; symbionts; Protozoa; genes; nucleotide sequences; molecular systematics; Reticulitermes santonensis; new species
Abstract:
... Lignocellulose digestion by wood-feeding termites depends on the mutualistic interaction of unusual, flagellate protists located in their hindgut. Most of the flagellates harbor numerous prokaryotic endosymbionts of so-far-unknown identity and function. Using a full-cycle molecular approach, we show here that the endosymbionts of the larger gut flagellates of Reticulitermes santonensis belong to t ...
Drosophila; bacteria; biodiversity; drought; extinction; fungi; global warming; heat; mammals; microclimate; models; photosynthesis; prediction; shrublands; solar radiation; sympatric speciation; viruses; Israel
Abstract:
... Climatic change and stress is a major driving force of evolution. The effects of climate change on living organisms have been shown primarily on regional and global scales. Here I propose the "Evolution Canyon" (EC) microscale model as a potential life monitor of global warming in Israel and the rest of the world. The EC model reveals evolution in action at a microscale involving biodiversity dive ...
athletic performance; attitudes and opinions; beverages; business enterprises; child nutrition; children; food consumption; food industry; food marketing; food recalls; purchasing; questionnaires; sports; teams
Abstract:
... BACKGROUND: Children's exposure to unhealthy food marketing influences their food knowledge, preferences and consumption. Sport sponsorship by food companies is widespread and industry investment in this marketing is increasing. This study aimed to assess children's awareness of sport sponsors and their brand-related attitudes and purchasing intentions in response to this marketing. METHODS: Sport ...
... We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus p ...
... BACKGROUND: Screen-viewing has been associated with increased body mass, increased risk of metabolic syndrome and lower psychological well-being among children and adolescents. There is a shortage of information about the nature of contemporary screen-viewing amongst children especially given the rapid advances in screen-viewing equipment technology and their widespread availability. Anecdotal evi ...
rivers; forest trees; wood; geomorphology; streams; riparian areas; ecosystems; case studies; New Zealand; Italy; California
Abstract:
... Although the geomorphic and ecological importance of large wood in streams and rivers is well recognized, most studies consider only dead wood in channels. However, we have observed that living parts of trees are often found within active channels and that this "livewood" shares functions with both instream dead wood and live riparian trees, while also providing some functions unique to living woo ...
... A specific marking and detection technique is a fundamental requirement for the safer use of genetically modified (GM) organisms. Here we propose a simple and effective method for directly marking functional transgenes in GM organisms. For that purpose, we introduced nucleotide substitutions (NS), based on the degeneracy of codons as markers (NS markers), into the bphC (2,3-dihydroxybiphenyl dioxy ...
... This article reviews recent research which shows that vanadium, nickel, silicon, fluorine, and tin are essential in animal nutrition. Since extrapolation of these data indicates that these elements may also be essential for man, the author states that inclusion of reasonable amounts of these trace elements in refined foods and food analogues may be desirable. ...
... Molecular cloning of the "old" but still unclassified Euonymus europaeus agglutinin (EEA) demonstrated that the lectin is a homodimeric protein composed of 152 residue subunits. Analysis of the deduced sequence indicated that EEA is synthesized without a signal peptide and undergoes no posttranslational processing apart from the removal of a six-residue N-terminal peptide. Glycan array screening c ...
alternative medicine; consciousness; focus groups; interviews; musculoskeletal system; patients; placebos
Abstract:
... BACKGROUND: Complementary therapists spend considerable time with their patients, especially in the first consultation. The communication between patients and their therapists is important for raising consciousness and activation of the patient’s self-healing power. Thus, the aims in this study were to delineate what complementary therapists regard as essential in patient consultations, their view ...
public lands; minorities (people); African Americans; forest recreation; recreation areas; Hispanic Americans; United States
Abstract:
... Recent data from the US Forest Service's onsite National Visitor Use Monitoring Survey (National Visitor Use Monitoring Survey, 2004. Unpublished demographic results for 2002-2003. Data on file with Donald English, Program Manager, Visitor Use Monitoring Project, Washington, DC) shows that visits made by African Americans account for very low percentages of visits to national forests across the co ...
... Elongated particles of simple RNA viruses of plants are composed of an RNA molecule coated with numerous identical capsid protein subunits to form a regular helical structure, of which tobacco mosaic virus is the archetype. Filamentous particles of the closterovirus beet yellows virus (BYV) reportedly contain approximately 4000 identical 22-kDa (p22) capsid protein subunits. The BYV genome encodes ...
... When we reach toward objects, we easily avoid potential obstacles located in the workspace. Previous studies suggest that obstacle avoidance relies on mechanisms in the dorsal visual stream in the posterior parietal cortex. One fundamental question that remains unanswered is where the visual inputs to these dorsal-stream mechanisms are coming from. Here, we provide compelling evidence that these m ...
adults; community programs; exercise; health promotion; men; psychosocial factors; questionnaires; regression analysis; social factors; social support; surveys; t-test; walking; women
Abstract:
... BACKGROUND: Conducting process evaluations of health promoting interventions, and measuring the effectiveness of specific intervention components, may help in the understanding of program failure or success. The purposes of the present study were to examine adults' exposure to and involvement in specific components of a three year long pseudo-experimental community-based physical activity interven ...
body mass index; children; eating habits; fathers; logit analysis; mothers; obesity; peers; physical activity; snacks; social environment; social networks; sports; surveys
Abstract:
... BACKGROUND: The hypothesis underlying this work is that the social network of a child might have an impact on the alimentary behaviors, in particular for what concerns snack consumption patterns. METHODS: 1215 Italian children 6-10 ys old were interviewed using a CATI facility in January 2010. 608 "snackers" and 607 "no-snackers" were identified. Information regarding family composition, child and ...
research projects; public opinion; farmers' attitudes; Nebraska
Abstract:
... Constraints to farmer participation in agricultural research require public institutions to compensate farmers for their involvement in research projects. Because of potential funding limitations in public institutions, it is important to identify approaches to compensate farmers who do not exclusively rely upon monetary remuneration. A novel barter compensation approach, called work-a-day compens ...
... Genetic data has become an essential part of ecological studies, because the analyses of diversity within and among natural populations may grant access to previously overlooked ecological and evolutionary causalities, especially among cryptic species. Here, we present an example of how phylogenetic analysis of molecular data obtained within a DNA barcoding study, in combination with morphological ...
anthropogenic activities; biodiversity; burning; farming systems; fire regime; foraging; habitats; image analysis; landscapes; lightning; lizards; remote sensing; resource management; space and time; women; Australia
Abstract:
... Aboriginal burning in Australia has long been assumed to be a "resource management" strategy, but no quantitative tests of this hypothesis have ever been conducted. We combine ethnographic observations of contemporary Aboriginal hunting and burning with satellite image analysis of anthropogenic and natural landscape structure to demonstrate the processes through which Aboriginal burning shapes ari ...
National Health and Nutrition Examination Survey; adults; body mass index; cardiovascular diseases; energy balance; exercise test; income; longitudinal studies; nationalities and ethnic groups; noninsulin-dependent diabetes mellitus; obesity; physical activity; risk factors; United States
Abstract:
... Low cardiovascular fitness is an independent risk factor for type 2 diabetes and cardiovascular disease in adults. The "fit but fat" concept suggests that cardiovascular fitness attenuates risk of metabolic and cardiovascular disease independent of body mass index (BMI), even among the obese. However, the proportion of U.S. adults considered both fit and obese is unknown. Thus, the purposes of thi ...
... Stem cells, their niches, and their relationship to cancer are under intense investigation. Because tumors and metastases acquire self-renewing capacity, mechanisms for their establishment may involve cell-cell interactions similar to those between stem cells and stem cell niches. On the basis of our studies in Caenorhabditis elegans, we introduce the concept of a "latent niche" as a differentiate ...
... BACKGROUND: Over the last two years, considerable advances have been made in common bean (Phaseolus vulgaris L.) genomics, especially with the completion of the genome sequence and the availability of RNAseq data. However, as common bean is recalcitrant to stable genetic transformation, much work remains to be done for the development of functional genomics tools adapted to large-scale studies. RE ...
... The enterococcal plasmid pKQ10 has been reported to carry a poorly characterized tetracycline resistance determinant designated tet(U). However, in a series of studies intended to further characterize this determinant, we have been unable to substantiate the claim that tet(U) confers resistance to tetracyclines. In line with these results, bioinformatic analysis provides compelling evidence that " ...
agricultural exhibitions; beef cattle; biosecurity; data analysis; income; poultry; public health; risk; swine; swine influenza; zoonoses
Abstract:
... Agricultural fairs provide one of the last frontiers, and largest stages, for showcasing livestock agriculture to the public. However, public funding, attendance revenue, animal biosecurity, and public health concerns are all aspects worthy of conversation and increased research attention given the interaction between livestock animals and the general public in fair and festival settings. A promin ...
alternative medicine; analysis of covariance; clinical trials; heart rate; inventories; mental health; pain; quality of life; questionnaires; women; yoga; New Zealand
Abstract:
... BACKGROUND: The neurobiology of persistent pain shares common underlying psychobiology with that of traumatic stress. Modern treatments for traumatic stress often involve bottom-up sensorimotor retraining/exposure therapies, where breath, movement, balance and mindfulness, are used to target underlying psychobiology. Vigorous exercise, in particular Bikram yoga, combines many of these sensorimotor ...
... BACKGROUND AND AIMS: Vascular epiphytes have to acquire nutrients from atmospheric wash out, stem-flow, canopy soils and trapped litter. Physiological studies on the adaptations to nutrient acquisition and plant utilization of nutrients have focused on phosphorus and nitrogen; potassium, as a third highly abundant nutrient element, has received minor attention. In the present study, potassium upta ...
random amplified polymorphic DNA technique; cultivars; Linum perenne; perennials; morphs; anthers; forbs; plant characteristics; stigma; color; indigenous species; plant reproduction; length; plant morphology; Linum lewisii; introduced plants; North America
Abstract:
... This article summarizes information on the taxonomy of 'Appar', a perennial blue flax cultivar (Linum perenne L. [Linaceae]), and characteristics that distinguish it from native Lewis flax (Linum lewisii Pursh [Linaceae]). 'Appar' apparently originated as a European flax that escaped from garden cultivation. Randomly amplified polymorphic DNA (RAPD) analysis places 'Appar' with other collections o ...
cultivars; Rubus; early development; agronomic traits; crop yield; firmness; pedigree
Abstract:
... Black Butte, a cross between ORUS 830-4 x ORUS 728-3, is an early, firm, attractive and extremely large-fruited trailing blackberry for the fresh or pick-your-own markets. The fruit are cylindrical/long conic, with drupelets of consistent size and shape, which give the fruit a very uniform appearance. The fruit are firmer than Marion and as firm as Waldo and Kotata. The fruit have excellent textur ...
... Blackberry cv. Thornless, selected from the cross Kotata × NZ 8610L-163 (E90 × N-71), is a thornless, high-yielding cultivar with good disease resistance and large, uniformly shaped and firm fruits with good processing quality and excellent adaptation to machine harvesting. ...
... Blackberry cv. Black Pearl, selected from the cross of ORUS 1117-11 × ORUS 1122-1, is a thornless cultivar with good plant disease resistance and large fruits with excellent processing quality and adaptation to machine harvesting. ...
... Bacteria-like endosymbionts of females of the plant-parasitic nematodes Globodera rostochiensis and Heterodera goettingiana and juveniles of Heterodera glycines were first observed during transmission electron microscopy (TEM) studies conducted in the 1970s. These organisms were characterized as being rod-shaped, ranging in size from 0.3 to 0.5 μm in diameter and 1.8 to 3 μm in length and containi ...
... Potato purple top wilt (PPT) is a devastating disease that occurs in various regions of North America and Mexico. At least three distinct phytoplasma strains belonging to three different phytoplasma groups (16SrI, 16SrII and 16SrVI) have been associated with this disease. A new disease with symptoms similar to PPT was recently observed in Texas and Nebraska, USA. Two distinct phytoplasma strain cl ...
Hibiscus rosa-sinensis; Phytoplasma; phytoplasmal diseases; witches' broom; new taxa; ribosomal DNA; nucleotide sequences; molecular systematics; restriction fragment length polymorphism; Brazil
Abstract:
... Hibiscus rosa-sinensis is a valuable ornamental species widely planted in Brazil. Many plants are affected by witches' broom disease, which is characterized by excessive axillary branching, abnormally small leaves, and deformed flowers, symptoms that are characteristic of diseases attributed to phytoplasmas. A phytoplasma was detected in diseased Hibiscus by amplification of rRNA operon sequences ...
... Chinook, a new primocane fruiting red raspberry (Rubus idaeus) from the US Department of Agriculture-Agricultural Research Service breeding programme in Oregon, was released in cooperation with the Oregon State Agricultural Experimental Station, the Washington State University Agricultural Research Center and the Idaho Agricultural Experiment Station. This high-yielding, early-ripening primocane c ...